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dough rheology and wet milling of hard waxy wheat flours.to realize the full potential of waxy wheat (triticum aestivum l.), the wet milling properties of waxy wheat flours including their dough-mixing properties were investigated. flours of six waxy hard wheats, one normal hard wheat ('karl 92'), and one partial waxy hard wheat ('trego') were fractionated by the dough-washing (martin) process, and the yields and recoveries of starch and gluten were compared. when waxy and normal wheat starches each were blended with a wheat gluten to give a mixture c ...200919594158
regulation of resistance and susceptibility in wheat-powdery mildew pathosystem with exogenous cytokinins.dose-response relationship between resistance of wheat seedlings (triticum aestivum, cultivar zarya) to erysiphe graminis f. sp. tritici marchal. (syn. blumeria graminis), a causal organism of wheat powdery mildew and exogenous zeatin has been investigated. two-week-old seedlings were inoculated with the pathogen. zeatin or zeatinriboside were added to the nutrient solution immediately after inoculation. the dose-response curve of cytokinin in the most cases was multiphasic, with peaks of increa ...200919592133
[studies on quantitative trait loci related to activity of lactate dehydrogenase in common carp (cyprinus carpio)].the reciprocal intergeneric hybrids between common wheat and roegneria kamoji were successfully obtained by means of embryo culture. morphology, chromosome pairing behavior at meiosis, fertility, and resistance to scab of the hybrid f1 and bc1 were studied. the results showed that the morphology of the reciprocal intergeneric hybrids f1 between r. kamoji and t. aestivum cv. chinese spring were intermediate type between the two parental species. the chromosome configuration at metaphase i (mi) of ...200919586894
molecular cloning and characterization of an up-regulated udp-glucosyltransferase gene induced by don from triticum aestivum l. cv. wangshuibai.fusarium head blight, also called scab, is a serious disease of small grain cereals and maize. scab can not only cause yield loss, more seriously is that it can also deteriorate seed quality by contaminating the infected grains with trichothecenes toxins harmful to human and animal health. deoxynivalenol (don) is one of the most important toxin members. it was proposed that don acted first as a virulence factor during fungal pathogenesis and then accumulated in grain to levels posing a threat to ...201019585272
cytological changes in turkish durum and bread wheat genotypes in response to salt stress.effects of salt stress on root growth, mitotic index, nuclear volume, vacuolization, nucleolar distortion and starch content were investigated in turkish bread wheat ( triticum aestivum l. cvs. yildiz - salt sensitive, dagdas - salt tolerant) and durum wheat ( triticum durum l. cvs. c1252 - salt sensitive, meramsalt tolerant) genotypes which were treated with 150 mm naci over a 6-day period. salt treatment of wheat seedlings resulted in a decrease in root elongation and cell division in all geno ...200919584031
variation in dehydration tolerance, aba sensitivity and related gene expression patterns in d-genome progenitor and synthetic hexaploid wheat lines.the wild wheat aegilops tauschii coss. has extensive natural variation available for breeding of common wheat. drought stress tolerance is closely related to abscisic acid (aba) sensitivity. in this study, 17 synthetic hexaploid wheat lines, produced by crossing the tetraploid wheat cultivar langdon with 17 accessions of ae. tauschii, were used for comparative analysis of natural variation in drought tolerance and aba sensitivity. ae. tauschii showed wide natural variation, with weak association ...200919582226
heterosis in some crosses of bread wheat under irrigation and drought conditions.five bread wheat varieties with different characteristics were crossed in a half-diallel model in 2005/2006 season. the five parents and their 10 f1 hybrids were evaluated under normal and water stress conditions during 2006/2007 season, to estimate heterosis of some wheat crosses for drought tolerance and selecting the crosses which could be useful in breeding programs for drought tolerance. the results indicated that, the best crosses over their mid parents at water stress condition for days t ...200919579996
gene effects and combining ability in some bread wheat genotypes to yellow rust disease.ten wheat lines were studied to determine gene effects and combining ability in some bread wheat genotypes to yellow rust disease. ten parental lines and f1 were evaluated in a randomized complete block design with three replications in agricultural and natural resources research center, mashhad, iran. two races (134e134a+ and 4e0a+) were used for this study. latent period (lp) and infection type (it) were measured in the field and greenhouse. results showed significant differences between races ...200919579935
genetic transformation of nepalese spring wheat (triticum aestivum l.) cultivars with ipt gene under the regulation of a senescence enhanced promoter from maize.two nepalese spring wheat cultivars were transformed with an ipt gene from agrobacterium tumefaciens under the control of the senescence inducible promoter psee1 from maize using biolistic method. the resulting transgenic lines, one from pasang lahmu and seven from annapurna-1, were studied for the expression of the transgene and the phenotype characters like chlorophyll content, chlorophyll a/b ratio, ps ii quantum yield and other parameters of agronomic importance. analysis of transgene expres ...200919579929
members of the dof transcription factor family in triticum aestivum are associated with light-mediated gene regulation.dna binding with one finger (dof) protein is a plant-specific transcription factor implicated in the regulation of many important plant-specific processes, including photosynthesis and carbohydrate metabolism. this study has identified 31 dof genes (tadof) in bread wheat through extensive analysis of current nucleotide databases. phylogenetic analysis suggests that the tadof family can be divided into four clades. expression analysis of the tadof family across all major organs using quantitative ...200919578911
benzoxazinoid biosynthesis, a model for evolution of secondary metabolic pathways in plants.benzoxazinoids are secondary metabolites that are effective in defence and allelopathy. they are synthesised in two subfamilies of the poaceae and sporadically found in single species of the dicots. the biosynthesis is fully elucidated in maize; here the genes encoding the enzymes of the pathway are in physical proximity. this "biosynthetic cluster" might facilitate coordinated gene regulation. data from zea mays, triticum aestivum and hordeum lechleri suggest that the pathway is of monophyletic ...200919577780
genomics in cereals: from genome-wide conserved orthologous set (cos) sequences to candidate genes for trait dissection.recent updates in comparative genomics among cereals have provided the opportunity to identify conserved orthologous set (cos) dna sequences for cross-genome map-based cloning of candidate genes underpinning quantitative traits. new tools are described that are applicable to any cereal genome of interest, namely, alignment criterion for orthologous couples identification, as well as the intron spanning marker software to automatically select intron-spanning primer pairs. in order to test the sof ...019575250
comparison of bloat potential between a variety of soft-red versus a variety of hard-red winter wheat forage.some aspects of wheat pasture bloat have been researched extensively, but few studies have evaluated the effect of wheat type or variety on bloat. eight gelbvieh x angus ruminally cannulated heifers (515 +/- 49 kg of bw) and 48 angus heifers (238 +/- 12 kg of bw) grazed 1-ha pastures of hard-red or soft-red winter wheat (triticum aestivum l.) to evaluate the effect of wheat variety on bloat potential. in exp. 1, cattle grazed from november 11 to 22 and from november 26 to december 7, 2006, in a ...200919574572
low-energy x-ray fluorescence microscopy opening new opportunities for bio-related research.biological systems are unique matter with very complex morphology and highly heterogeneous chemical composition dominated by light elements. discriminating qualitatively at the sub-micrometer level the lateral distribution of constituent elements, and correlating it to the sub-cellular biological structure, continues to be a challenge. the low-energy x-ray fluorescence microspectroscopy, recently implemented in twinmic scanning transmission mode, has opened up new opportunities for mapping the d ...200919570794
[effects of shading on wheat grain starch quality and redistribution of pre-anthesis stored nonstructural carbohydrates].this paper studied the effects of shading in the period from jointing to maturity on the grain yield, starch content, and starch paste traits of two winter wheat (triticum aestivum l.) cultivars, shading-tolerant yangmai 158 and shading-intolerant yangmai 11, and analyzed the relationships of the redistribution of total soluble sugars stored before anthesis in vegetative organs with the grain yield, starch content, and starch paste parameters of the cultivars. the results showed that shading dec ...200919565759
phosphorylation at s384 regulates the activity of the taalmt1 malate transporter that underlies aluminum resistance in wheat.in this study we examined the role of protein phosphorylation/dephosphorylation in the transport properties of the wheat (triticum aestivum) root malate efflux transporter underlying al resistance, taalmt1. pre-incubation of xenopus laevis oocytes expressing taalmt1 with protein kinase inhibitors (k252a and staurosporine) strongly inhibited both basal and al(3+)-enhanced taalmt1-mediated inward currents (malate efflux). pre-incubation with phosphatase inhibitors (okadaic acid and cyclosporine a) ...200919563436
isolation and heterologous transformation analysis of a pollen-specific promoter from wheat (triticum aestivum l.).the promoter of a pollen-specific gene tapsg719 was isolated from wheat (triticum aestivum l.) by inverse-pcr (ipcr). sequence analysis revealed that the promoter contains two cis-acting elements (agaaa and gtga) known to confer anther/pollen-specific gene expression which suggests that the promoter of tapsg719 gene is a pollen-specific one. to ascertain the regulatory function of tapsg719 promoter, two deleted fragments (-1,776 to -1 bp and -1,019 to -1 bp) were fused to the beta-glucuronidase ...201019562512
phytotoxicity tests of solid wastes and contaminated soils in the czech republic.the purpose of this study was to compare the suitability of different phytotoxicity testing procedures for the evaluation of toxicity associated with both soil contamination and solid wastes, both of which can be of environmental risk to plants. ten different representative types of contaminated soils and solid waste samples were chosen from the czech republic.201019557447
wheat-alien metaphase i pairing of individual wheat genomes and d genome chromosomes in interspecific hybrids between triticum aestivum l. and aegilops geniculata roth.homoeologous metaphase i (mi) pairing of triticum aestivum x aegilops geniculata hybrids (2n = 5x = 35, abdu(g)m(g)) has been examined by an in situ hybridization procedure permitting simultaneous discrimination of a, b, d and wild genomes. the seven d genome chromosomes (and their arms, except for 6d and 7d) plus some additional wheat chromosomes were also identified. wheat-wild mi associations represented more than 60% of total, with an average ratio of 5:1:12 for those involving the a, b and ...200919557382
eco-toxic effects of sulfadiazine sodium, sulfamonomethoxine sodium and enrofloxacin on wheat, chinese cabbage and tomato.investigation of the toxic effects of three veterinary drugs [sulfadiazine sodium (sds), sulfamonomethoxine sodium (smms), and enrofloxacin (eflx)] on seed germination, root elongation and shoot elongation of wheat (triticum aestivum l.), chinese cabbage (brassica campestris l.) and tomato (cyphomandra betacea) was carried out. significant linear relationships between the root and shoot elongation and the concentration of veterinary drugs addition were observed. the effects of the three veterina ...200919554446
identification of genomic regions determining the phenological development leading to floral transition in wheat (triticum aestivum l.).autumn-seeded winter cereals acquire tolerance to freezing temperatures and become vernalized by exposure to low temperature (lt). the level of accumulated lt tolerance depends on the cold acclimation rate and factors controlling timing of floral transition at the shoot apical meristem. in this study, genomic loci controlling the floral transition time were mapped in a winter wheat (t. aestivum l.) doubled haploid (dh) mapping population segregating for lt tolerance and rate of phenological deve ...019553371
transcriptional profiling in response to terminal drought stress reveals differential responses along the wheat genome.water stress during grain filling has a marked effect on grain yield, leading to a reduced endosperm cell number and thus sink capacity to accumulate dry matter. the bread wheat cultivar chinese spring (cs), a chinese spring terminal deletion line (cs_5al-10) and the durum wheat cultivar creso were subjected to transcriptional profiling after exposure to mild and severe drought stress at the grain filling stage to find evidences of differential stress responses associated to different wheat geno ...200919552804
[freezing tolerance of winter wheat plants depends on adaptation of photosynthesis and respiration in different time intervals].this study is devoted to co2 gas exchange (true photosynthesis at light saturation (p), dark respiration (r), and p/r ratio) in vegetating and cold-hardened winter wheat (triticum aestivum l.) plants (cultivar mironovskaya 808) in relation to their freezing tolerance. under natural cultivation conditions, freezing tolerance of plants depended on adaptive changes in the shape of p and r curves in the temperature range from 20 to -2 degrees c. these changes, induced by cold hardening and treatment ...201619551968
effects of dried distillers' grains with solubles (wheat-based) in feedlot cattle diets on feces and manure composition.the use of dried distillers' grains with solubles (ddgs) in feedlot cattle (bos taurus) diets is increasing as the bio-ethanol industry expands. this study investigated how wheat (triticum aestivum l.) ddgs-based diets impact feedlot cattle nutrient and volatile fatty acid (vfa) excretion. feedlot heifers were fed ddgs at 0 (control) 20, 40, 60% or 60% + ca (1% limestone) of dietary dry matter. feces and manure were sampled monthly over a 133-d finishing period. total nitrogen (tn) (feces only), ...200919549948
the dawgpaws pipeline for the annotation of genes and transposable elements in plant genomes.abstract:200919545381
uptake and acropetal translocation of polycyclic aromatic hydrocarbons by wheat (triticum aestivum l.) grown in field-contaminated soil.uptake and acropetal translocation of 14 priority polycyclic aromatic hydrocarbons (pahs) by wheat (triticum aestivum l.) grown in 15 field-contaminated soils were investigated in a growth chamber. pah concentrations in roots correlated positively with the corresponding concentrations in soils and negatively with the contents of soil organic carbon (p < 0.01). no clear linear relationship was found between log rcf (root concentration factor, microg g(-1) root/microg g(-1) soil on dry weight basi ...200919544854
integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat.a rye-wheat centric chromosome translocation 1rs.1bl has been widely used in wheat breeding programs around the world. increased yield of translocation lines was probably a consequence of increased root biomass. in an effort to map loci-controlling root characteristics, homoeologous recombinants of 1rs with 1bs were used to generate a consensus genetic map comprised of 20 phenotypic and molecular markers, with an average spacing of 2.5 cm. physically, all recombination events were located in the ...200919544051
cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (triticum aestivum).nitrogen (n) remobilization in wheat (triticum aestivum) plants is crucial because it determines the grain protein concentration and the baking quality of flour. in order to evaluate the influence of cytokinins on n remobilization during n starvation, we analyzed various n remobilization parameters in wheat plants that were watered with 6-benzylaminopurine (bap) either with or without kno(3). besides, the effects of bap on protein synthesis were evaluated, and the size and ultrastructure of chlo ...200919540618
cytogenetic and molecular identification of three triticum aestivum-leymus racemosus translocation addition lines.chromosome 2c from aegilops cylindrica has the ability to induce chromosome breakage in common wheat (tritivum aestivum). in the bc(1)f(3) generation of the t. aestivum cv. chinese spring and a hybrid between t. aestivum-leymus racemosus lr.7 addition line and t. aestivum-ae. cylindrica 2c addition line, three disomic translocation addition lines (2n = 44) were selected by mitotic chromosome c-banding and genomic in situ hybridization. we further characterized these t. aestivum-l. racemosus tran ...200919539248
effects of overexpression of high molecular weight glutenin subunit 1dy10 on wheat tortilla properties.wheat (triticum aestivum l.) flour properties necessary for optimal tortilla production have not been identified. transgenic wheats (triticum aestivum l.) overexpressing high molecular weight glutenin subunit (hmw-gs) 1dy10 were used to make tortilla and their quality was evaluated. the level of hmw-gs 1dy10 in flours derived from transgenic wheats was 2.5-5.8-fold greater than in controls. polymeric proteins in the transgenic samples had a molecular weight distribution shifted toward larger pol ...200919537784
[cytological and ssr analysis of irradiation-induced progenies of trititrigia substitution line sn0095].pollens of trititrigia substitution line sn0095 were radiated with 60co-gamma ray and cytological characters of the progenies m1 and m2 were studied. the results showed that variations with different frequencies and chromosome numbers (2n=41, 2n=40 and 2n=39) were observed in both m1 and m2. abnormal phenomena, such as univalents, multivalents, chromosome fragments, laggard chromosomes, chromosome bridges and micronucleus, appeared in high frequencies during the meiosis. it suggested that irradi ...200919537191
assessment of adaptive evolution between wheat and rice as deduced from full-length common wheat cdna sequence data and expression patterns.wheat is an allopolyploid plant that harbors a huge, complex genome. therefore, accumulation of expressed sequence tags (ests) for wheat is becoming particularly important for functional genomics and molecular breeding. we prepared a comprehensive collection of ests from the various tissues that develop during the wheat life cycle and from tissues subjected to stress. we also examined their expression profiles in silico. as full-length cdnas are indispensable to certify the collected ests and an ...019534823
plasmolysis and cell wall deposition in wheat root hairs under osmotic stress.we analysed cell wall formation in rapidly growing root hairs of triticum aestivum under reduced turgor pressure by application of iso- and hypertonic mannitol solutions. our experimental series revealed an osmotic value of wheat root hairs of 150 mosm. in higher concentrations (200-650 mosm), exocytosis of wall material and its deposition, as well as callose synthesis, still occurred, but the elongation of root hairs was stopped. even after strong plasmolysis when the protoplast retreated from ...201019533299
characterization of plastidial starch phosphorylase in triticum aestivum l. endosperm.starch phosphorylase (pho) catalyses the reversible transfer of glucosyl units from glucose1-phosphate to the non-reducing end of an alpha-1,4-linked glucan chain. two major isoforms of pho exist in the plastid (pho1) and cytosol (pho2). in this paper it is proposed that pho1 may play an important role in recycling glucosyl units from malto-oligosaccharides back into starch synthesis in the developing wheat endosperm. pho activity was observed in highly purified amyloplast extracts prepared from ...200919524321
cloning, identification, expression analysis and phylogenetic relevance of two nadp-dependent malic enzyme genes from hexaploid wheat.the nadp-dependent malic enzyme (nadp-me; ec1.1.1.40) found in many metabolic pathways catalyzes the oxidative decarboxylation of l-malate, producing pyruvate, co(2) and nadph. the nadp-mes have been well studied in c4 plants but not well in c3 plants. in this study, we identified the nadp-me isoforms from hexaploid wheat (triticum aestivum l). two different nadp-me transcripts were first identified in this c3 plant. the first is named tanadp-me1 [ncbi: eu170134] and encodes a putative plastidic ...200919523599
reactive oxygen species production in wheat roots is not linked with changes in h fluxes during acidic and aluminium stresses.aluminium stress induces peroxidation of lipids in the plasma membrane, the effect akin to that caused by reactive oxygen species (ros). ros have recently been proposed as regulators of redox-dependent ion transport across the plasma membrane during biotic and abiotic stresses, thus contributing to the plant defence system. the aim of this study was to discover whether ros production is linked to redox-dependent h(+) transport system located at the plasma membranes of two near-isogenic lines of ...200619521479
mapping of quantitative trait loci for grain iron and zinc concentration in diploid a genome wheat.micronutrients, especially iron (fe) and zinc (zn), are deficient in the diets of people in underdeveloped countries. biofortification of food crops is the best approach for alleviating the micronutrient deficiencies. identification of germplasm with high grain fe and zn and understanding the genetic basis of their accumulation are the prerequisites for manipulation of these micronutrients. some wild relatives of wheat were found to have higher grain fe and zn concentrations compared with the cu ...201319520762
the membrane topology of almt1, an aluminum-activated malate transport protein in wheat (triticum aestivum).the wheat almt1 gene encodes an aluminum (al)-activated malate transport protein which confers al-resistance. we investigated the membrane topology of this plasma-membrane localized protein with immunocytochemical techniques. several green fluorescent protein (gfp)-fused and histidine (his)-tagged chimeras of almt1 were prepared based on a computer-predicted secondary structure and transiently expressed in cultured mammalian cells. antibodies raised to polypeptide epitopes of almt1 were used in ...200719517008
meiosis drives extraordinary genome plasticity in the haploid fungal plant pathogen mycosphaerella graminicola.meiosis in the haploid plant-pathogenic fungus mycosphaerella graminicola results in eight ascospores due to a mitotic division following the two meiotic divisions. the transient diploid phase allows for recombination among homologous chromosomes. however, some chromosomes of m. graminicola lack homologs and do not pair during meiosis. because these chromosomes are not present universally in the genome of the organism they can be considered to be dispensable. to analyze the meiotic transmission ...200919516898
molecular cloning and characterization of a novel glyoxalase i gene tagly i in wheat (triticum aestivum l.).methylglyoxal is a kind of poisonous metabolite that can react with rna, dna and protein, which generally results in a number of side advert effects to cell. glyoxalase i is a member of glyoxalase system that can detoxify methylglyoxal. an est encoding a glyoxalase i was isolated from a ssh (suppression subtractive hybridization)-cdna library of wheat spike inoculated by fusarium graminearum. the corresponding full length gene, named tagly i, was cloned, sequenced and characterized. its genomic ...201019513813
characterization of the wheat endosperm transfer cell-specific protein tapr60.the tapr60 gene from bread wheat encodes a small cysteine-rich protein with a hydrophobic signal peptide, predicted to direct the tapr60 protein to a secretory pathway. it was demonstrated by heterologous expression of recombinant tapr60 protein that the signal peptide is recognized and cleaved in yeast cells. the full-length gene including promoter sequence of a tapr60 orthologue was cloned from a bac library of triticum durum. a transcriptional promoter-gus fusion was stably transformed into w ...200919513805
a quantitative genetic study for elucidating the contribution of glutamine synthetase, glutamate dehydrogenase and other nitrogen-related physiological traits to the agronomic performance of common wheat.to better understand the genetic variability for nitrogen use efficiency in winter wheat is a necessity in the frame of the present economic and ecological context. the objective of this work was to investigate the role of the enzymes glutamine synthetase (gs) and glutamate dehydrogenase (gdh), and other nitrogen (n)-related physiological traits in the control of agronomic performance in wheat. a quantitative genetics approach was developed using the arche x récital population of doubled haploid ...200919513687
hydrogen peroxide scavenging regulates germination ability during wheat (triticum aestivum l.) seed maturation.hydrogen peroxide (h(2)o(2)) promotes seed germination of cereal plants and ascorbic acid which acts as antioxidant suppresses the germination of wheat seeds, but the role of h(2)o(2) scavenging on germination during seed maturation has not been demonstrated. we investigated relationship of germination, ascorbate, h(2)o(2) scavenging enzymes and sensitivity to ascorbic acid (asa) maturing seeds of two typical wheat (triticum aestivum l.) cultivars, cvs. shirogane-komugi and norin61. shirogane-ko ...200819513213
[triton x-100 induces heritable changes of morphological characters in triticum aestivum l].the effect of the nonionic detergent polyethylene glycol octylphenyl ester (triton x-100, tx-100) on the spring common wheat cultivar alem was studied under laboratory and field conditions. treatment of seeds and vegetating plants with 0.1 or 0.01% tx-100 (aqueous solution) changed the spike morphology in all plants of the first posttreatment generation. the changes were inherited by the second generation without additional treatment with tx-100. square-headed dense spikes with doubled spikelets ...200919507710
novel micrornas uncovered by deep sequencing of small rna transcriptomes in bread wheat (triticum aestivum l.) and brachypodium distachyon (l.) beauv.the small rna transcriptomes of bread wheat and its emerging model brachypodium distachyon were obtained by using deep sequencing technology. small rna compositions were analyzed in these two species. in addition to 70 conserved micrornas (mirnas) from 25 families, 23 novel wheat mirnas were identified. for brachypodium, 12 putative mirnas were predicted from a limited number of expressed sequence tags, of which one was a potential novel mirna. also, 94 conserved mirnas from 28 families were ide ...200919499258
evidence for variation in the optimal translation initiation complex: plant eif4b, eif4f, and eif(iso)4f differentially promote translation of mrnas.eukaryotic initiation factor (eif) 4b is known to interact with multiple initiation factors, mrna, rrna, and poly(a) binding protein (pabp). to gain a better understanding of the function of eif4b, the two isoforms from arabidopsis (arabidopsis thaliana) were expressed and analyzed using biophysical and biochemical methods. plant eif4b was found by ultracentrifugation and light scattering analysis to most likely be a monomer with an extended structure. an extended structure would facilitate the ...200919493973
effects of molybdenum on expression of cold-responsive genes in abscisic acid (aba)-dependent and aba-independent pathways in winter wheat under low-temperature stress.molybdenum (mo) is an essential trace element for higher plants. it has been shown that application of mo enhances the cold resistance of winter wheat. in order to improve our understanding of the molecular mechanisms of cold resistance arising from application of mo in winter wheat, investigations were made regarding the transcription of cold-responsive (cor) genes in abscisic acid (aba)-dependent and aba-independent pathways in winter wheat regulated by mo application under low-temperature str ...200919491090
application of capillary electrophoresis to determine the technological properties of wheat flours by a glutenin index.capillary electrophoresis was used to characterize glutenin proteins from ancient varieties of southern italy common wheat and to determine the technological properties of wheat flours based on a glutenin index. three zones were identified in the electropherograms, indicated as a, b, and c according to electroelution order. the three zones corresponded to the low molecular weight glutenin subunits and to the y- and x-type high molecular weight subunits, respectively. the ratio b/c was correlated ...201619490316
class d and b(sister) mads-box genes are associated with ectopic ovule formation in the pistil-like stamens of alloplasmic wheat (triticum aestivum l.).homeotic transformation of stamens into pistil-like structures (pistillody) has been reported in cytoplasmic substitution (alloplasmic) lines of bread wheat (triticum aestivum l.) that have the cytoplasm of a related wild species, aegilops crassa. an ectopic ovule differentiates in the pistil-like stamen in the alloplasmic wheat. the seedstick (stk)-like class d mads-box gene, wheat stk (wstk), was expressed in the primordia of ectopic ovules in the pistil-like stamens as well as in the true pis ...200919488678
molecular characterisation of the low-molecular weight glutenin subunit genes of tall wheatgrass and functional properties of one clone ee34.wild tall wheatgrass (lophopyrum elongatum l., 2x = 14) is an important resource for improving bread wheat (titicum aestivum l.), including hmw-gs and lmw-gs relevant to end-use quality of the wheat flour. a set of 14 distinct sequences were amplified from the genomic dna of the tall wheatgrass, using degenerate primers targeted at glu-3, the locus containing the genes encoding the low-molecular weight glutenin subunits (lmw-gs). three sequences contained an internal stop codon and were classifi ...201019484339
rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection.a marker-assisted background selection (mabs)-based gene introgression approach in wheat (triticum aestivum l.) was optimized, where 97% or more of a recurrent parent genome (rpg) can be recovered in just two backcross (bc) generations. a four-step mabs method was developed based on 'plabsim' computer simulations and wheat genome structure information. during empirical optimization of this method, double recombinants around the target gene were selected in a step-wise fashion during the two bc c ...200919484121
rapid development of pcr-based genome-specific repetitive dna junction markers in wheat.in hexaploid wheat (triticum aestivum l.) (aabbdd, c=17 000 mb), repeat dna accounts for approximately 90% of the genome, of which transposable elements (tes) constitute 60%-80%. despite the dynamic evolution of tes, our previous study indicated that the majority of tes are conserved and collinear between the homologous wheat genomes, based on identical insertion patterns. in this study, we exploited the unique and abundant te insertion junction regions identified from diploid aegilops tauschii ...200919483776
a sublethal dose of thiamethoxam causes a reduction in xylem feeding by the bird cherry-oat aphid (rhopalosiphum padi), which is associated with dehydration and reduced performance.the active ingestion of xylem sap by aphids is hypothesised to be an important mechanism for rehydration. when starved bird cherry-oat aphids (rhopalosiphum padi) were allowed to feed on wheat (triticum aestivum) treated with a sublethal dose of the xylem-mobile neonicotinoid thiamethoxam, analysis of feeding behaviours using the electrical penetration graph revealed a reduction in xylem feeding that was reversed on removal of the toxin. to test the importance of xylem-feeding behaviour as a reh ...200919482292
overexpression of tastrg gene improves salt and drought tolerance in rice.high salt and drought are the main factors affecting agricultural production. thus, cloning stress-tolerance-related genes and identifying their functions are essential to enhancing crop tolerance to stresses. in this study, a salt-induced unknown wheat (triticum aestivum l.) gene was identified and cloned according to microarray analysis of salt-tolerant wheat mutant rh8706-49 under salt stress. the gene was named triticum aestivum salt tolerance-related gene (tastrg) and submitted to genbank ( ...200919481835
different levels of humoral immunoreactivity to different wheat cultivars gliadin are present in patients with celiac disease and in patients with multiple myeloma.immunity to food antigens (gliadin, cow's milk proteins) is in the centre of the attention of modern medicine focused on the prevention of diseases, prevention which is based on the use of appropriate restriction diet. detection of the enhanced levels of the immune reactions to antigen(s) present in food is from this point of view of great importance because there are reports that some of health disturbances, like celiac disease (cd) and some premalignant conditions, like monoclonal gammopathy o ...200919480718
discovery and mapping of single feature polymorphisms in wheat using affymetrix arrays.wheat (triticum aestivum l.) is a staple food crop worldwide. the wheat genome has not yet been sequenced due to its huge genome size (approximately 17,000 mb) and high levels of repetitive sequences; the whole genome sequence may not be expected in the near future. available linkage maps have low marker density due to limitation in available markers; therefore new technologies that detect genome-wide polymorphisms are still needed to discover a large number of new markers for construction of hi ...200919480702
prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat.prometryne is one of the herbicides widely used for controlling weed/grass in agricultural practice. however, whether it has an adverse effect on crops is unknown. in this study, we investigated prometryne-induced oxidative stress in wheat (triticum aestivum). wheat plants were grown in soils with prometryne at 0-24 mgkg(-1) soil. the growth of wheat treated with prometryne was inhibited. chlorophyll content significantly decreased even at the low level of prometryne (4 mgkg(-1) soil). accumulat ...200919473703
molecular identification of a new powdery mildew resistance gene pm41 on chromosome 3bl derived from wild emmer (triticum turgidum var. dicoccoides).powdery mildew caused by blumeria graminis f. sp. tritici is an important wheat disease in china and other parts of the world. wild emmer (triticum turgidum var. dicoccoides) is the immediate progenitor of cultivated tetraploid and hexaploid wheats and thus an important resource for wheat improvement. wild emmer accession iw2 collected from mount hermon, israel, is highly resistant to powdery mildew at the seedling and adult plant stages. genetic analysis using an f(2) segregating population and ...200919471905
markers to a common bunt resistance gene derived from 'blizzard' wheat (triticum aestivum l.) and mapped to chromosome arm 1bs.common bunt, caused by tilletia caries (dc.) tul. & c. tul. and t. laevis j.g kuhn, is an economically important disease of wheat (triticum aestivum l.) worldwide. the resistance in the winter wheat cultivar 'blizzard' is effective against known races of common bunt in western canada. the incorporation of resistance from blizzard into field-ready cultivars may be accelerated through the use of molecular markers. using the maize pollen method, a doubled haploid population of 147 lines was develop ...200919471904
unlocking wheat genetic resources for the molecular identification of previously undescribed functional alleles at the pm3 resistance locus.the continuous improvement of crop plants is essential for agriculture in the coming decades and relies on the use of genetic variability through breeding. however, domestication and modern breeding have reduced diversity in the crop germplasm. global gene banks conserve diversity, but these resources remain underexplored owing to a lack of efficient strategies to isolate important alleles. here we describe a large-scale allele-mining project at the molecular level. we first selected a set of 1, ...200919470492
physical-chemical analysis of non-polyphenol oxidase (non-ppo) darkening in yellow alkaline noodles.darkening in yellow alkaline noodles (yan) was measured over 24 h in a high polyphenol oxidase (ppo) bread wheat ( triticum aestivum l. cv. tasman) and a very low ppo durum wheat ( triticum durum cv. kamilaroi). over 24 h non-ppo darkening occurred across a range of ph 3.5-10.5, and in tasman this was overlaid by darkening from ppo activity. the rate of darkening in yan was separated into two main time periods, 0-4 and 4-24 h. the first 4 h of darkening was further divided into two stages using ...200919469560
oligosaccharin and aba synergistically affect the acquisition of freezing tolerance in winter wheat.in this paper, we continue our studies of the previously discovered [o.a. zabotina, d.a. ayupova, o.n. larskaya, o.n. nikolaeva, g.i. petrovicheva, a.i. zabotin, physiologically active oligosaccharides, accumulating in the roots of winter wheat during adaptation to low temperature, russian journal of plant physiology 45 (1998) 262] oligosaccharin (physiologically active oligosaccharide) gxag, which stimulates the acquisition of freezing tolerance in winter varieties of triticum aestivum l. the t ...200919467881
transgenic approach to improve wheat (triticum aestivum l.) nutritional quality.an amaranth (amaranthus hypochondriacus) albumin gene, encoding the 35-kda ama1 protein of the seed, with a high content of essential amino acids, was used in the biolistic transformation of bread wheat (triticum aestivum l.) variety cadenza. the transformation cassette carried the ama1 gene under the control of a powerful wheat endosperm-specific promoter (1bx17 hmw-gs). southern-blot analysis of t(1) lines confirmed the integration of the foreign gene, while rt-pcr and western-blot analyses of ...200919466426
leaching and utilization of nitrogen during a spring wheat catch crop succession.an experiment covering a 2-yr spring wheat (triticum aestivum l.) catch crop succession was conducted in lysimeters to account for the losses of n due to leaching. we sought to relate these losses to the n uptake of the main crop and to integrate the estimated n loss and uptake into a balance. the non-winter hardy catch crops [yellow mustard (sinapis alba l.), phacelia (phacelia tanacetifolia benth), and sunflower (helianthus annuus l.)] as well as bare soil fallow were studied at low and high n ...201319465716
algerian pearl millet ( pennisetum glaucum l.) contains xip but not taxi and tlxi type xylanase inhibitors.an xip (xylanase inhibiting protein) type xylanase inhibitor was purified from algerian pearl millet ( pennisetum glaucum l.) grains and characterized for the first time. cation exchange and affinity chromatography with immobilized trichoderma longibrachiatum glycoside hydrolase (gh) family 11 xylanase resulted in electrophoretically pure protein with a molecular mass of 27-29 kda and a pi value of 6.7. the experimentally determined n-terminal amino acid sequence of the purified xip protein is 8 ...200919459708
study on programmed cell death and dynamic changes of starch accumulation in pericarp cells of triticum aestivum l.features of programmed cell death (pcd) and dynamic changes of starch accumulation in developing pericarp cells of wheat (triticum aestivum l.) were observed and analyzed by periodic acid-schiff/toluidine blue o double staining, fluorescence staining, terminal deoxynucleotidyl transferase-mediated fluorescein deoxyuridine triphosphate nick-end labeling (tunel) and transmission electron microscopy. the results showed that cellular organelles were orderly disintegrated. tunel-positive nuclei were ...200919455280
dissimilar molecular defense responses are elicited in triticum aestivum after infestation by different diuraphis noxia biotypes.for sustainable solutions to the problem of insect infestation, the study of molecular plant-insect interactions is integral to resistance breeding strategies. this also holds true in the case of wheat (triticum aestivum), where the russian wheat aphid (diuraphis noxia, kurdjumov, rwa) is responsible for significant crop losses in most major wheat producing countries around the world. our study is focused on gaining a greater understanding of the resistance mechanisms activated by the rwa resist ...200919453509
defense strategy of old and modern spring wheat varieties during soil drying.different defense mechanisms of three spring wheat (triticum aestivum l.) varieties were studied by withholding watering in well-watered pots to gradually increase water deficit of plants grown in containers. the strategies of plant adaptation were divided into three phases according to the severity of drought: first, a positive defense phase that started from commencement of non-hydraulic root-sourced signals (nhrs) and ended at onset of hydraulic root-sourced signals (hrs)--the plant responded ...200919453498
differentiation of molecular genotypes and virulence phenotypes of puccinia triticina from common wheat in north america.wheat leaf rust caused by puccinia triticina is widely distributed in the wheat growing regions of the united states and canada, and is subject to selection for virulence phenotype by leaf rust resistance genes in wheat cultivars. the objective of this study was to determine the number of genetically differentiated groups of p. triticina that are currently present in north america. in total, 148 isolates of p. triticina from the 1980s to 2005 were collected from wheat-growing regions of the unit ...200919453235
virulence analysis of hessian fly populations from texas, oklahoma, and kansas.in recent years, the number of wheat, triticum aestivum l., fields heavily infested by hessian fly, mayetiola destructor (say), has increased in the great plains of the united states. historically, resistance genes in wheat have been the most efficient means of controlling this insect pest. to determine which resistance genes are still effective in this area, virulence of six hessian fly populations from texas, oklahoma, and kansas was determined, using the resistance genes h3, h4, h5, h6, h7h8, ...200919449660
development of triticum aestivum-leymus racemosus translocation lines using gametocidal chromosomes.specific chromosomes of certain aegilops species introduced into wheat genome background may often facilitate chromosome breakage and refusion, and finally result in a variety of chromosome restructuring. such a phenomenon is commonly called gametocidal effect of the chromosomes. the chromosome 2c of ae. cylindrica is one of such chromosomes. in the present study, scab resistant wheat-l. racemosus addition lines involving chromosomes lr.2 and lr.7 were crossed to wheat-ae. cylindrica disomic add ...200319448992
qtl-specific microarray gene expression analysis of wheat resistance to fusarium head blight in sumai-3 and two susceptible nils.fusarium head blight, predominantly caused by fusarium graminearum (schwabe) in north america, is a destructive disease that poses a serious threat to wheat (triticum aestivum l.) production around the world. cdna microarrays consisting of wheat ests derived from a wheat - f. graminearum interaction suppressive subtractive hybridization library were used to investigate qtl-specific differential gene expression between the resistant chinese cultivar sumai-3 and two susceptible near isogenic lines ...200919448721
trifldb: a database of clustered full-length coding sequences from triticeae with applications to comparative grass genomics.the triticeae full-length cds database (trifldb) contains available information regarding full-length coding sequences (cdss) of the triticeae crops wheat (triticum aestivum) and barley (hordeum vulgare) and includes functional annotations and comparative genomics features. trifldb provides a search interface using keywords for gene function and related gene ontology terms and a similarity search for dna and deduced translated amino acid sequences to access annotations of triticeae full-length c ...200919448038
induction and transmission of wheat-haynaldia villosa chromosomal translocations.in order to develop more wheat-haynaldia villosa translocations involving different chromosomes and chromosome segments of h. villosa, t. durum-h. villosa amphiploid was irradiated with 60co gamma-rays at doses of 800, 1,200, and 1,600 rad. pollen collected from the spikes 1, 2, and 3 days after irradiation were transferred to emasculated spikes of the common wheat cv. 'chinese spring'. genomic in situ hybridization was used to identify wheat-h. villosa chromosome translocations in the m1 genera ...200919447380
ecotoxicological effects of paracetamol on seed germination and seedling development of wheat (triticum aestivum l.).in order to assess ecological risk of pharmaceutical compounds entering into agricultural ecosystems, toxic effects of paracetamol with therapeutic action on wheat (triticum aestivum l.) were investigated as an example, using early growing and developmental indexes of wheat, including seed germination, shoot height and root length, peroxidase, superoxide dismutase, chlorophyll, and soluble protein in the seedlings. the results showed that wheat shoot and root elongation decreased significantly ( ...200919446955
a host-selective toxin of pyrenophora tritici-repentis, ptr toxa, induces photosystem changes and reactive oxygen species accumulation in sensitive wheat.ptr toxa (toxa) is a proteinaceous necrotizing host-selective toxin produced by pyrenophora tritici-repentis, a fungal pathogen of wheat (triticum aestivum). in this study, we have found that treatment of toxa-sensitive wheat leaves with toxa leads to a light-dependent accumulation of reactive oxygen species (ros) that correlates with the onset of necrosis. furthermore, the accumulation of ros and necrosis could be inhibited by the antioxidant n-acetyl cysteine, providing further evidence that r ...200919445591
effects of heavy-ion beams on chromosomes of common wheat, triticum aestivum.to investigate the nature of plant chromosomes irradiated by heavy-ion beams, the effects of nitrogen (n) and neon (ne) ion beams on hexaploid wheat chromosomes were compared with those of x-ray. chromosome aberrations, such as short, ring and dicentric chromosomes appeared in high frequency. the average numbers of chromosome breaks at ld-50 by irradiation with x-ray, n and ne ion beams were 32, 20 and 20, respectively. these values may be underestimated because chromosome rearrangement without ...200919442674
neuroprotective effects of triticum aestivum l. against beta-amyloid-induced cell death and memory impairments.beta-amyloid (a beta) is a key component of senile plaques, neuropathological hallmarks of alzheimer's disease (ad) and has been reported to induce cell death via oxidative stress. this study investigated the protective effects of triticum aestivum l. (tal) on a beta-induced apoptosis in sh-sy5y cells and cognitive dysfunctions in sprague-dawley (sd) rats. cells treated with a beta exhibited decreased viability and apoptotic features, such as dna fragmentation, alterations in mitochondria and an ...201019441012
meta-qtl analysis of the genetic control of ear emergence in elite european winter wheat germplasm.variation in ear emergence time is critical for the adaptation of wheat (triticum aestivum l.) to specific environments. the aim of this study was to identify genes controlling ear emergence time in elite european winter wheat germplasm. four doubled haploid populations derived from the crosses: avalon x cadenza, savannah x rialto, spark x rialto, and charger x badger were selected which represent diversity in european winter wheat breeding programmes. ear emergence time was recorded as the time ...200919430758
molecular markers based identification of diversity for drought tolerance in bread wheat varieties and synthetic hexaploids.the complexity of the wheat genome has delayed the development and application of molecular markers to this species and wheat now lies behind barley, maize and rice in marker development. however, improvements in marker detection systems and in the techniques used to identify markers linked to useful traits has allowed considerable advances to be made in recent years. to evaluate the genetic diversity 53 genotypes of richard's selection, were studied at national agriculture reseach center (narc) ...200919430030
comparative expression of cbf genes in the triticeae under different acclimation induction temperatures.in plants, the c-repeat binding factors (cbfs) are believed to regulate low-temperature (lt) tolerance. however, most functional studies of cbfs have focused on characterizing expression after an lt shock and have not quantified differences associated with variable temperature induction or the rate of response to lt treatment. in the triticeae, rye (secale cereale l.) is one of the most lt-tolerant species, and is an excellent model to study and compare cbf lt induction and expression profiles. ...019421778
dissection of barley chromosome 3h in common wheat and a comparison of 3h physical and genetic maps.we used the gametocidal system to dissect a barley chromosome 3h added to common wheat. the gametocidal system induced chromosomal structural changes in the 3h addition line of common wheat, and we cytologically screened for rearranged chromosomes involving the 3h chromosome by in situ hybridization (fish/gish). we established 50 common wheat lines carrying single rearranged (or dissected) 3h chromosomes of independent origin. the dissected 3h chromosomes were either deletions or translocations ...200919420798
the c-glycosylation of flavonoids in cereals.flavonoids normally accumulate in plants as o-glycosylated derivatives, but several species, including major cereal crops, predominantly synthesize flavone-c-glycosides, which are stable to hydrolysis and are biologically active both in planta and as dietary components. an enzyme (oscgt) catalyzing the udp-glucose-dependent c-glucosylation of 2-hydroxyflavanone precursors of flavonoids has been identified and cloned from rice (oryza sativa ssp. indica), with a similar protein characterized in wh ...019411659
identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (triticum turgidum var. dicoccoides).powdery mildew, caused by blumeria graminis f. sp. tritici, is one of the most important wheat diseases worldwide in areas with cool or maritime climates. wild emmer (triticum turgidum var. dicoccoides) is an important potential donor of disease resistances and other traits for common wheat improvement. a powdery mildew resistance gene was transferred from wild emmer accession g-303-1m to susceptible common wheat by crossing and backcrossing, resulting in inbred line p63 (yanda1817/g-303-1 m//3* ...200919407985
scarlet-rz1, an ems-generated hexaploid wheat with tolerance to the soilborne necrotrophic pathogens rhizoctonia solani ag-8 and r. oryzae.the necrotrophic root pathogens rhizoctonia solani ag-8 and r. oryzae cause rhizoctonia root rot and damping-off, yield-limiting diseases that pose barriers to the adoption of conservation tillage in wheat production systems. existing control practices are only partially effective, and natural genetic resistance to rhizoctonia has not been identified in wheat or its close relatives. we report the first genetic resistance/tolerance to r. solani ag-8 and r. oryzae in wheat (triticum aestivum l. em ...200919407984
identification of novel qtls for seedling and adult plant leaf rust resistance in a wheat doubled haploid population.pyramiding of genes that confer partial resistance is a method for developing wheat (triticum aestivum l.) cultivars with durable resistance to leaf rust caused by puccinia triticina. in this research, a doubled haploid population derived from the cross between the synthetic hexaploid wheat (shw) (xaegilotriticum spp.) line ta4152-60 and the north dakota breeding line nd495 was used for identifying genes conferring partial resistance to leaf rust in both the adult plant and seedling stages. five ...200919396420
delayed wheat flag leaf senescence due to removal of spikelets is associated with increased activities of leaf antioxidant enzymes, reduced glutathione/oxidized glutathione ratio and oxidative damage to mitochondrial proteins.removal of reproductive 'sink' i.e. spikelets from wheat at anthesis delays the rate of flag leaf senescence. in this work, the antioxidant defense was studied in the flag leaf of triticum aestivum cv. kalyansona plants showing normal (s + plants) and delayed senescence via removal of spikelets (s- plants). this was done by measurement of metabolites and activities of enzymes such as superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase, monodehydroascorbate reductase, dehydr ...200919394842
survey of stink bug (hemiptera: pentatomidae) egg parasitoids in wheat, soybean, and vegetable crops in southeast virginia.stink bugs (hemiptera: pentatomidae) cause significant damage to many different crops and horticultural commodities in virginia. however, little is known about the species diversity or impact of stink bug egg parasitoids in the state. a survey was conducted in 2005 and 2006 (may through september) in wheat (triticum aestivum l.), soybean (glycine max l.), and several vegetable crops by collecting natural egg masses of various stink bug species and by monitoring sentinel egg masses. a total of 57 ...200919389285
low crop plant population densities promote pollen-mediated gene flow in spring wheat (triticum aestivum l.).transgenic wheat is currently being field tested with the intent of eventual commercialization. the development of wheat genotypes with novel traits has raised concerns regarding the presence of volunteer wheat populations and the role they may play in facilitating transgene movement. here, we report the results of a field experiment that investigated the potential of spring wheat plant population density and crop height to minimize gene flow from a herbicide-resistant (hr) volunteer population ...200919387859
water movement into dormant and non-dormant wheat (triticum aestivum l.) grains.the movement of water into harvest-ripe grains of dormant and non-dormant genotypes of wheat (triticum aestivum l.) was investigated using magnetic resonance micro-imaging (mrmi). images of virtual sections, both longitudinal and transverse, throughout the grain were collected at intervals after the start of imbibition and used to reconstruct a picture of water location within the different grain tissues and changes over time. the observations were supplemented by the weighing measurements of wa ...200919386615
differential protection of ethylenediurea (edu) against ambient ozone for five cultivars of tropical wheat.the antiozonant edu (ethylenediurea) was used to assess the impact of ambient o(3) under field conditions on five cultivars of tropical wheat (triticum aestivum l.). edu solution (0 ppm and 400 ppm) was applied as soil drench (100 ml plant(-1)) 10 days after germination (dag) at an interval of 12 days. edu-treated plants showed significant increments in stomatal conductance, photosynthetic rate, variable fluorescence, total chlorophyll, ascorbic acid, proline and protein contents and protective ...201319386404
the gamma-gliadin multigene family in common wheat (triticum aestivum) and its closely related species.the unique properties of wheat flour primarily depend on gluten, which is the most important source of protein for human being. gamma-gliadins have been considered to be the most ancient of the wheat gluten family. the complex family structure of gamma-gliadins complicates the determination of their function. moreover, gamma-gliadins contain several sets of celiac disease epitopes. however, no systematic research has been conducted yet.200919383144
[a study of the storage proteins in the introgression lines of common wheat (triticum aestivum l. x t. timopheevii zhuk.) resistant to brown leaf rust].storage proteins, prolamins, were studied in ten introgression lines of common wheat bred with involvement of triticum timopheevii zhuk. and five commercial hexaploid wheat cultivars. the lines are resistant to leaf rust. a comparative analysis of the storage proteins in the triticum aestivum l. introgression lines and the parental forms allowed us to (1) detect the active genes of prolamins on the chromosomes homeologous groups 1 and 6 in the introgression lines of t. aestivum and t. timopheevi ...200919382688
[marker characters of variation induced in triticum aestivum l. by nicotinic acid].a search for markers of epigenetic variability in spring common wheat form genotroph-1, obtained after the treatment of seeds and growing plants of spring common wheat cultivar kazakhstanskaya-126 with nicotinic acid of innate origin (0.1-0.01% water solutions), and in a winter wheat line gostianum-88 along with a common wheat cultivar alem obtained on the basis of genotroph-1 was conducted under laboratory conditions. the possibility of using isoenzymes as markers for studying variation induced ...200919382687
arsenate toxicity for wheat and lettuce in six chinese soils with different properties.to assess soil arsenic (as, in the form of arsenate) toxicity to plants, 6-d root elongation tests on wheat (triticum aestivum l.) and lettuce (lactuca sativa) were conducted in six chinese soils freshly spiked with as. plants were treated with 7 or 10 levels of as to establish concentration-effect curves. median effective concentration and 10% effective concentration values were derived with regression analysis to measure as toxic potencies, and no-observed-effect concentration was determined b ...200919379017
molecular cytogenetic and agronomic characterization of advanced generations of wheat x triticale hybrids resistant to diuraphis noxia (mordvilko): application of gish and microsatellite markers.the pi 386148 triticale from russia is among the highest resistant line to the russian wheat aphid (rwa) (diuraphis noxia (mordvilko)). this triticale line was used as the male parent in crosses with lamar wheat (triticum aestivum l.). the f1 plants were backcrossed to lamar wheat. the progenies were tested for rwa biotype 1 reaction for at least eight backcross and selfing generations. five lines from these selections were identified for their resistance to the rwa and their seeds were increase ...200919370091
assignment of aegilops variabilis eig chromosomes and translocations carrying resistance to nematodes in wheat.the allotetraploid species aegilops variabilis eig (2n = 28, uusvsv) belongs to the tribe triticeae and is closely related to wheat. one accession, ae. variabilis no. 1, was found to be resistant to the cereal cyst nematode (ccn) and the root-knot nematode (rkn). as the genetic variability for resistance to those two pests is limited within wheat, this accession was crossed to bread wheat. previous work enabled the development of two addition lines and two translocation lines carrying resistance ...200919370089
evolution of new disease specificity at a simple resistance locus in a crop-weed complex: reconstitution of the lr21 gene in wheat.the wheat leaf-rust resistance gene lr21 was first identified in an iranian accession of goatgrass, aegilops tauschii coss., the d-genome donor of hexaploid bread wheat, and was introgressed into modern wheat cultivars by breeding. to elucidate the origin of the gene, we analyzed sequences of lr21 and lr21 alleles from 24 wheat cultivars and 25 accessions of ae. tauschii collected along the caspian sea in iran and azerbaijan. three basic nonfunctional lr21 haplotypes, h1, h2, and h3, were identi ...200919364806
mitochondrial and nuclear localization of a novel pea thioredoxin: identification of its mitochondrial target proteins.plants contain several genes encoding thioredoxins (trxs), small proteins involved in the regulation of the activity of many enzymes through dithiol-disulfide exchange. in addition to chloroplastic and cytoplasmic trx systems, plant mitochondria contain a reduced nicotinamide adenine dinucleotide phosphate-dependent trx reductase and a specific trx o, and to date, there have been no reports of a gene encoding a plant nuclear trx. we report here the presence in pea (pisum sativum) mitochondria an ...200919363090
simultaneous selection of major and minor genes: use of qtl to increase selection efficiency of coleoptile length of wheat (triticum aestivum l.).plant breeders simultaneously select for qualitative traits controlled by one or a small number of major genes, as well as for polygenic traits controlled by multiple genes that may be detected as quantitative trait loci (qtl). in this study, we applied computer simulation to investigate simultaneous selection for alleles at both major and minor gene (as qtl) loci in breeding populations of two wheat parental lines, hm14bs and sunstate. loci targeted for selection included six major genes affect ...200919360392
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