Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [a quantitative analysis of conidia production in aspergillus nidulans]. | 1974 | 4463418 | |
| regulation of the pentose phosphate pathway in the fungus aspergillus nidulans. | 1972 | 4403947 | |
| relationship between energy substrate utilization and specific growth rate in aspergillus nidulans. | the maintenance coefficient of glucose-limited aspergillus nidulans chemostat cultures at 30 c was 0.018 g per g (dry weight) per hr for glucose and 0.55 mmoles per g (dry weight) per hr for oxygen. these values can only be approximate because melanin was produced by the mold at low growth rates and because it is unlikely that this polymer contributed to the maintenance energy requirement although it contributed to the dry weight. biomass (defined here as dry weight minus melanin) was used to ca ... | 1971 | 4399338 |
| attempts to detect an alternative vital role for the reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase structural gene in aspergillus nidulans. | a possible alternate vital role of the structural gene for reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase in aspergillus nidulans was tested for and found to be absent in a significant number of haploid and diploid strains. | 1971 | 4397637 |
| characterization of the reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase of aspergillus nidulans. | the reduced nicotinamide adenine dinucleotide phosphate (nadph)-nitrate oxidoreductase (ec 1.6.6.2) from aspergillus nidulans was purified over 200-fold by use of salt fractionation, gel filtration, and ion-exchange chromatography. the purified enzyme was specific for nadph and catalyzed reduction of nitrate, cytochrome c from isolated mitochondria of aspergillus, and mammalian cytochrome c. an s(0.725) (20, w) of 7.8 was derived with sucrose density gradient centrifugation, and a stokes radius ... | 1971 | 4396143 |
| control of gene action in aspergillus nidulans. | 1970 | 4395097 | |
| the stoicheiometry of electron transfer by bacterial and plant ferredoxins. | 1. the number of electrons carried by ferredoxins from spinach, the blue-green alga anacystis nidulans, the anaerobic bacterium clostridium welchii and the photosynthetic bacterium chromatium was determined. 2. ferredoxins were reduced by illuminated chloroplasts, and the stoicheiometry of the reoxidation in the dark of the ferredoxins by nadp and benzyl viologen was measured. 3. spinach and a. nidulans ferredoxins were found to be one-electron carriers, and cl. welchii and chromatium ferredoxin ... | 1968 | 4387387 |
| genetic and biochemical studies of nitrate reduction in aspergillus nidulans. | 1. in aspergillus nidulans nitrate and nitrite induce nitrate reductase, nitrite reductase and hydroxylamine reductase, and ammonium represses the three enzymes. 2. nitrate reductase can donate electrons to a wide variety of acceptors in addition to nitrate. these artificial acceptors include benzyl viologen, 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride, cytochrome c and potassium ferricyanide. similarly nitrite reductase and hydroxylamine reductase (which are possibly a singl ... | 1967 | 4382427 |
| mutants of aspergillus nidulans impaired in penicillin biosynthesis. | 1974 | 4375170 | |
| a new method for the preparation of uniformly 14c-labelled compounds by using anacystis nidulans. | 1. growth conditions were devised which enabled anacystis nidulans to be grown on high specific radioactivity (14)co(2) (at over 98% isotopic abundance of (14)c) in a closed system with greater than 85% substrate utilization. 2. the dna and rna content of a. nidulans was estimated by various methods and compared with that of chlorella pyrenoidosa. 3. a procedure was developed for the quantitative extraction and separation of the dna and rna from a. nidulans. 4. the fractionation and analysis of ... | 1974 | 4374187 |
| genetic control of the sensitivity of aspergillus nidulans to mutagenic factors. ii. the sensitivity of uvs mutants to different mutagenic factors. | 1974 | 4367713 | |
| the regulation of nad l-glutamate dehydrogenase in aspergillus nidulans. | 1974 | 4366241 | |
| [ultrastructure of the nucleus-associated body ("centriolar plaque", "mtoc", "kce") during interphase in the conidia of aspergillus nidulans]. | 1974 | 4365430 | |
| conidiophore and spore development in aspergillus nidulans. | 1972 | 4347343 | |
| genetic and allied effects of certain esters of inorganic acids in aspergillus nidulans. | 1971 | 4336682 | |
| molybdate metabolism in aspergillus nidulans. ii. mutations affecting phosphatase activity or galactose utilization. | 1970 | 4319916 | |
| adenosine triphosphatase of aspergillus nidulans: activation by calcium ion & inhibition of this effect by magnesium ion. | 1974 | 4279888 | |
| physiological and biochemical studies of nuclear and extranuclear oligomycin-resistant mutants of aspergillus nidulans. | 1974 | 4278766 | |
| onychomycosis in elderly chiropody patients. | 1974 | 4277596 | |
| regulation of the pentose phosphate pathway in the fungus aspergillus nidulans. the effect of growth with nitrate. | 1974 | 4274591 | |
| a study of the kinetics of hyphal extension and branch initiation of fungal mycelia. | 1974 | 4274556 | |
| formation of hybrid anthranilate synthetase in vitro from components of aspergillus and neurospora. | 1973 | 4269431 | |
| spontaneous mutations in fungi. | 1972 | 4265097 | |
| [mycotoxins produced by aspergillus. their action on the chick embryo]. | 1969 | 4259296 | |
| [on the frequency of aflatoxin and aspergillus in foods]. | 1969 | 4259173 | |
| chemical induction of mitotic recombination in aspergillus nidulans. | 1971 | 4254990 | |
| the pinkish red pigment produced by an adenineless mutant of aspergillus nidulans. | 1969 | 4245435 | |
| induction of inter- and intragenic mitotic recombination by fluorodeoxyuridine and fluorouracil in aspergillus nidulans. | 1967 | 4226822 | |
| fungal growth rate and the formation of ethylene in soil. | 1974 | 4215868 | |
| rapid screening for the isolation of mutants of aspergillus nidulans with increased penicillin yields. | 1974 | 4215785 | |
| the genetics of penicillium chrysogenum. | 1973 | 4212418 | |
| mutational analysis of dark endogenous metabolism in the blue-green bacterium anacystis nidulans. | we describe a mutant (strain 704) of the obligate photoautotroph anacystis nidulans which behaves like the wild type under continuous illumination but which in the dark rapidly loses viability, respires little, and incorporates label into ribonucleic acid and protein at rates considerably less than observed with the darkened wild type. extracts of this mutant strain show no detectable 6-phosphogluconate dehydrogenase (ec 1.1.1.44) activity. spontaneous revertants of mutant 704 were selected as s ... | 1974 | 4212183 |
| [the fibrinolytic and thrombolytic activity of the mold fungi of the genus aspergillus]. | 1973 | 4205355 | |
| precursor of 5s ribosomal ribonucleic acid in the blue-green alga anacystis nidulans. | the maturation of 5s ribosomal ribonucleic acid (rrna) in the obligately photoautotrophic unicellular blue-green alga anacystis nidulans has been studied by using polyacrylamide gel electrophoresis and t1 ribonuclease oligonucleotide analysis. a. nidulans mature 5s rrna (m5) is of approximately the same molecular weight as the 5s rrna of escherichia coli, and is derived by cleavage of a precursor (p5) containing a few (three to six) additional nucleotides. some of these additional nucleotides oc ... | 1974 | 4204437 |
| effect of osmotic concentration and ph on sclerotia and cleistothecia production in alkaline and fertile soil aspergilli. | 1973 | 4201565 | |
| the neutral and alkaline proteases of aspergillus nidulans. | 1973 | 4201327 | |
| the isolation of tyrosinase from aspergillus nidulans, its kinetic and molecular properties and some consideration of its activity in vivo. | 1973 | 4198643 | |
| mitosis in aspergillus nidulans. | 1969 | 4188529 | |
| karyokinesis of the somatic nucleus of aspergillus nidulans. ii. nuclear events during hyphal differentiation. | 1968 | 4180480 | |
| coordinate regulation of enzyme inducibility and developmental competence in aspergillus nidulans. | 1974 | 4156015 | |
| the role of malic enzyme in aspergillus nidulans. | 1974 | 4153143 | |
| properties of the assimilatory nitrate reductase from aspergillus nidulans. | 1974 | 4153024 | |
| the effect of the carbon source on ammonium regulation in aspergillus nidulans. | 1974 | 4150856 | |
| the effects of carbon source on glutamate dehydrogenase activities in aspergillus nidulans. | 1974 | 4150766 | |
| the genetic control of molybdoflavoproteins in aspergillus nidulans. ii. use of nadh dehydrogenase activity associated with xanthine dehydrogenase to investigate substrate and product inductions. | 1973 | 4149466 | |
| nad and nadp l-glutamate dehydrogenase activity and ammonium regulation in aspergillus nidulans. | 1973 | 4148302 | |
| ammonium regulation in aspergillus nidulans. | l-glutamate uptake, thiourea uptake, and methylammonium uptake and the intracellular ammonium concentration were measured in wild-type and mutant cells of aspergillus nidulans held in various concentrations of ammonium and urea. the levels of l-glutamate uptake, thiourea uptake, nitrate reductase, and hypoxanthine dehydrogenase activity are determined by the extracellular ammonium concentration. the level of methylammonium uptake is determined by the intracellular ammonium concentration. the upt ... | 1973 | 4145865 |
| the multimeric nature of nadph-nitrate reductase from aspergillus nidulans. | 1973 | 4144577 | |
| the role of molybdenum in formation of the nadph-nitrate reductase by aspergillus nidulans. | 1973 | 4144009 | |
| repression of enzymes of nitrogen catabolism by methylammonium and caesium chloride in strains of aspergillus nidulans insensitive to ammonium repression. | 1974 | 4138442 | |
| localization of free sulphydryl groups by ferricyanide reduction in developing conidiophores of aspergillus nidulans. | 1974 | 4134662 | |
| cytochemical detection of hydrolases in fungus cells. 3. aryl sulfatase. | 1974 | 4132629 | |
| osteomyelitis and pneumonia in a boy with chronic granulomatous disease of childhood caused by a mutant strain of aspergillus nidulans. | 1974 | 4131011 | |
| the effects of griseofulvin on mitosis in aspergillus nidulans. | 1972 | 4113458 | |
| ultrastructure of dormant and germinating conidia of aspergillus nidulans. | 1972 | 4110693 | |
| the use of amides as nitrogen sources by aspergillus nidulans. | 1970 | 4102613 | |
| the genetic analysis of regulation of amidase synthesis in aspergillus nidulans. ii. mutants resistant to fluoroacetamide. | 1970 | 4097637 | |
| the mitochondrial genome of the fission yeast schizosaccharomyces pombe. the cytochrome b gene has an intron closely related to the first two introns in the saccharomyces cerevisiae cox1 gene. | the dna sequence of the cob region of the schizosaccharomyces pombe mitochondrial dna has been determined. the cytochrome b structural gene is interrupted by an intron of 2526 base-pairs, which has an open reading frame of 2421 base-pairs in phase with the upstream exon. the position of the intron differs from those found in the cob genes of saccharomyces cerevisiae, aspergillus nidulans or neurospora crassa. the sch. pombe cob intron has the potential of assuming an rna secondary structure almo ... | 1985 | 4046021 |
| mutagenicity of three agricultural soils. | a chemical and biological testing protocol was employed to evaluate the mutagenic potential of the organic compounds extracted from three agricultural soils. the analytical procedures used included bioassays with salmonella typhimurium and aspergillus nidulans for the detection of point mutations and a gas chromatography/mass spectrometry/computer system to identify major organic constituents. the extracts of all three soils exhibited mutagenic response in the bioassays. at a dose level of 1000 ... | 1985 | 3983630 |
| the sub-cellular localisation and regulatory properties of pyruvate carboxylase from rhizopus arrhizus. | cell-free extracts of rhizopus arrhizus contain exclusively cytosolic pyruvate carboxylase and nad-glutamate dehydrogenase, a single mitochondrial isoenzyme of nadp-isocitrate dehydrogenase, and both mitochondrial and cytosolic isoenzymes of nadp-malate dehydrogenase (decarboxylating). other enzymes examined have sub-cellular localisations similar to those characteristic of mammalian liver. purified preparations of r. arrhizus pyruvate carboxylase are subject to partial regulatory inhibition by ... | 1985 | 3971971 |
| transformation of aspergillus nidulans. | 1985 | 3939985 | |
| dna metabolism during infection of anacystis nidulans by cyanophage as-1. vii. uv-induced alterations of the as-1/a. nidulans lytic cycle. | in order to interfere specifically with either the host of phage dna metabolism and separate the effects of new phage dna synthesis from the effects of host cell breakdown and pil-dna formation, uv irradiation of either the host, a. nidulans, or intact phage as-1, prior to infection was utilized. several conclusions were reached. first, a photoreactivation system was present in uv-irradiated a. nidulans. second, the complete burst size of as-1 was severely affected by uv irradiation of cells and ... | 1985 | 3938514 |
| dna metabolism during infection of anacystis nidulans by cyanophage as-1. vi. effect of hydroxyurea and nalidixic acid on the development of cyanophage as-1. | the use of the dna inhibitors hydroxyurea (hu) and nalidixic acid (nal) to elucidate patterns of dna metabolism in as-1 infected a. nidulans have led to several conclusions. first, hu and nal at concentrations of 500 and 100 micrograms/ml, respectively, inhibited dna synthesis in synchronized a. nidulans. protein and chlorophyll synthesis remained essentially unchanged as did turbidity increases. second, the complete burst size of the cyanophage as-1 was severely affected by hu and nal treatment ... | 1985 | 3938513 |
| mycotoxins producing fungi and mycoflora of air-dust from taif, saudi arabia. | using the dilution plate method, 70 species and 31 genera were collected from 20 dust samples on glucose (28 genera and 64 species) and cellulose czapek's agar (22 genera and 46 species) at 28 degrees c. the most common fungi were aspergillus niger, a. flavus, a. flavus var. columnaris, phoma glomerata, fusarium oxysporum, penicillium chrysogenum and mucor racemosus; and a. nidulans, phoma humicola, drechslera spicifera and stachybotrys chartarum on the two media, respectively. toxicity test sho ... | 1985 | 3935928 |
| variation of a 470 000 daltons antigen complex and catalase antigen in clinical isolates of aspergillus fumigatus. | antigens in ruptured mycelium of 18 aspergillus strains including 14 clinical isolates of a. fumigatus were studied by immunoelectrophoresis. one antigenic component of molecular weight 470 000 previously characterized by hydrophobic interaction chromatography and gel filtration and a second component with catalase activity were detected in all a. fumigatus isolates but in varying quantities. the 470 000 antigen complex cross-reacted with antigens in a. flavus and a. nidulans but not in a. niger ... | 1985 | 3934773 |
| organization of pigment proteins in the photosystem ii complex of the cyanobacterium anacystis nidulans r2. | two chlorophyll-protein complexes associated with photosystem ii (psii) of the cyanobacterium anacystis nidulans r2 have been detected. the larger of the two complexes, cpvi-1, contained a 71-kda and a 42-kda protein. the 71-kda protein was determined to be the anchor protein of the phycobilisomes (the light-harvesting complex of a. nidulans psii), since it was recognized by an antibody raised against a similar protein from another cyanobacterium. the second complex, cpvi-4, contained a previous ... | 1985 | 3931080 |
| inhibition of nitrate utilization by amino acids in intact anacystis nidulans cells. | in an attempt to establish the nature of the ammonium-assimilation products which mediate the inhibition by ammonium of nitrate uptake in cyanobacteria, the effect of different amino acids on nitrate utilization by intact anacystis nidulans cells has been assayed. to exclude an indirect inhibition of nitrate uptake through the ammonium which the amino acids might release, the cells were pretreated with l-methionine-d,l-sulfoximine (msx), a potent inactivator of glutamine synthetase. under these ... | 1985 | 3929744 |
| microinjected photoreactivating enzymes from anacystis and saccharomyces monomerize dimers in chromatin of human cells. | photoreactivating enzymes (pre) from the yeast saccharomyces cerevisiae and the cyanobacterium anacystis nidulans have been injected into the cytoplasm of repair-proficient human fibroblasts in culture. after administration of photoreactivation light, pre-injected cells displayed a significantly lower level of uv-induced unscheduled dna synthesis (uds) than non-injected cells. this indicates that monomerization of the uv-induced pyrimidine dimers in the mammalian chromatin had occurred as a resu ... | 1985 | 3923332 |
| dependence of nitrate utilization upon active co2 fixation in anacystis nidulans: a regulatory aspect of the interaction between photosynthetic carbon and nitrogen metabolism. | specific inhibition of photosynthetic co2 fixation in anacystis nidulans cells by d,l-glyceraldehyde resulted in the simultaneous inhibition of nitrate utilization, indicating a dependence of the latter process upon the provision of co2-fixation products. this dependence was lost in cells treated with l-methionine-d,l-sulfoximine or azaserine, effective inhibitors of ammonium assimilation. in these cells, nitrate uptake could proceed at rates similar to those in control cells even if co2 fixatio ... | 1985 | 3919645 |
| identification of the acia gene controlled by the amda regulatory gene in aspergillus nidulans. | the amda gene is one of a number of transacting regulatory genes controlling expression of the amds gene in a. nidulans. a polypeptide of approximately 42,000 molecular weight has been found to be synthesized constitutively in amda mutant strains and to be acetate inducible. a lambda clone containing a gene, called acia, coding for this polypeptide has been isolated using differential screening with cdna probes. two acetate inducible rna species have been identified by probing northern blots wit ... | 1985 | 3916805 |
| gene amplification in aspergillus nidulans by transformation with vectors containing the amds gene. | conidial protoplasts of an a. nidulans amds deletion strain (mh1277) have been transformed to the amds+ phenotype with a plasmid carrying the wild type gene (p3sr2). optimalisation of transformation and plating conditions now has resulted in frequencies of 300-400 transformants per microgram of dna. analysis of dna from amds+ transformants of mh1277 showed that transformation had occurred by integration of vector dna sequences into the genome. in virtually all these transformants multiple copies ... | 1985 | 3916728 |
| cloning and characterization of the three enzyme structural genes qutb, qutc and qute from the quinic acid utilization gene cluster in aspergillus nidulans. | heterologous dna probes from the quinic acid gene cluster (qa) in neurospora crassa (schweizer 1981) have been used to isolate the corresponding gene cluster (qut) from aspergillus nidulans cloned in a phage lambda vector. n. crassa probes for each of the three enzyme structural genes in the cluster have been used to identify the corresponding genes within the a. nidulans cloned dna. the three genes are in the same relative sequence [dehydrogenase (1), qa-3 = qutb; dehydratase (3), qa-4 = qutc; ... | 1985 | 3916726 |
| the product of the regulatory gene of the proline catabolism gene cluster of aspergillus nidulans is a positive-acting protein. | eight new deletion mutations in the prn gene cluster involved in l-proline catabolism in aspergillus nidulans have been characterised and mapped. three of these are located within prna, the regulatory gene mediating proline induction, and confirm the positive nature of the action of the prna product. in addition, four prna- alleles which are phenotypically suppressible by aminoglycoside antibiotics have been identified. of these four phenotypically suppressible prna- mutations, two have been tes ... | 1985 | 3916725 |
| molecular cloning of the 3-phosphoglycerate kinase (pgk) gene from aspergillus nidulans. | the aspergillus nidulans 3-phosphoglycerate kinase gene (pgk) has been isolated from a phage lambda genomic library, using the equivalent yeast gene as a hybridization probe. the location of the pgk gene within the cloned dna has been physically mapped. the dna sequence of a small region of the putative pgk has been determined and found to code for amino acids corresponding to the n-terminal end of the pgk protein. in contrast to the yeast pgk gene the aspergillus gene contains a 57 base pair in ... | 1985 | 3916724 |
| the aspergillus nidulans mitochondrial genome. | a brief description is provided of the overall organisation of the aspergillus nidulans mitochondrial genome, as revealed by dna sequence analysis. | 1985 | 3916717 |
| the purification of urease from aspergillus nidulans. | a purification procedure is described for aspergillus urease, the most important step being affinity chromatography on hydroxyurea sepharose. the enzyme exists as a single active species of mr 240,000. the pure enzyme has an activity of 670 mumol urea hydrolysed/min, has a km of 10(-3) m, an optimum ph of 8.5 and a sub-unit mr of 40,000. | 1985 | 3912228 |
| nucleotide sequence encoding the biosynthetic dehydroquinase function of the penta-functional arom locus of aspergillus nidulans. | the nucleotide sequence of a 1.9 kb hindiii fragment of dna derived from the arom locus of a.nidulans and encoding the biosynthetic dehydroquinase activity has been determined. the sequences encoding the biosynthetic and catabolic dehydroquinase enzymes of a.nidulans show no detectable homology, strongly suggesting convergent evolutionary pathways. the messenger rna specified by the arom locus was detected as a 5.3 kb rna species. | 1985 | 3906567 |
| mitochondria and nuclei move by different mechanisms in aspergillus nidulans. | we have examined the effects of the antimicrotubule agent benomyl and several mutations on nuclear and mitochondrial movement in germlings of the filamentous fungus aspergillus nidulans. while, as previously reported, benomyl inhibited nuclear division and movement, it did not inhibit mitochondrial movement. to test the effects of benomyl more rigorously, we germinated two benomyl super-sensitive, beta-tubulin mutants at a benomyl concentration 50-100 times greater than that required to inhibit ... | 1985 | 3905827 |
| isolation and characterization of cold-sensitive mutations at the bena, beta-tubulin, locus of aspergillus nidulans. | we have isolated large numbers of conditionally lethal beta-tubulin mutations to provide raw material for analyzing the structure and function of beta tubulin and of microtubules. we have isolated such mutations as intragenic suppressors of bena33, a heat-sensitive (hs-) beta-tubulin mutation of aspergillus nidulans. among over 2,600 revertants isolated, 126 were cold-sensitive (cs-). in 41 of 78 cs- revertants analyzed, cold sensitivity and reversion from hs- to hs+ were due to mutations linked ... | 1985 | 3903435 |
| transformation of aspergillus niger using the argb gene of aspergillus nidulans. | a mutant of aspergillus niger defective in ornithine transcarbamylase function was transformed with plasmids carrying a functional copy of the argb gene of aspergillus nidulans after treatment of spheroplasts in the presence of polyethylene glycol and calcium ions. the plasmid pdg3 gave stable transformants at a frequency of 4 per microgram of input dna. southern blot analysis of dna from transformants showed that pdg3 dna had integrated into the a. niger chromosomes at a variety of locations. t ... | 1985 | 3902571 |
| identification and functional analysis of beta-tubulin genes by site specific integrative transformation in aspergillus nidulans. | we have cloned two different beta-tubulin sequences from the filamentous fungus aspergillus nidulans. each was used in the construction of transforming plasmids that carry the pyr4 gene of neurospora crassa. we used these plasmids to transform a pyrg-strain of aspergillus to uridine prototrophy. both plasmids were shown to integrate site specifically into the homologous chromosomal sequences. we then used transformant strains in genetic crosses to demonstrate that one of the cloned beta-tubulin ... | 1985 | 3897247 |
| involvement of a particular species of beta-tubulin (beta 3) in conidial development in aspergillus nidulans. | strains of aspergillus containing the bena22 mutation are resistant to benomyl for vegetative growth but do not produce conidia. to test whether conidiation involved an additional benomyl-sensitive tubulin (i.e., was mediated by a tubulin other than the tubulins coded for by the bena locus), a collection of mutants was produced that formed conidia in the presence of benomyl, i.e., were conidiation-resistant (cr-) mutants. we analyzed the tubulins of these cr- mutants using two-dimensional gel el ... | 1985 | 3897246 |
| a conductimetric method for assaying asparaginase activity in aspergillus nidulans. | aspergillus nidulans asparaginase activity may be assayed conductimetrically. the method is based on the increase of conductivity which is due to the production of ammonia and/or aspartate in a reaction mixture containing a. nidulans cell-free extract and asparagine or aspartate hydroxamate. this conductivity is linear with time and enzyme concentration and it follows michaelis kinetics. conductimetric activity was not detectable in mutants lacking asparaginase activity. | 1985 | 3896790 |
| mycetoma caused by aspergillus nidulans in india. | the first case of mycetoma caused by aspergillus nidulans has been described from india in a young farmer of jaisalmer situated in the thar desert of western rajasthan, india. the diagnosis was confirmed by histopathological and mycological studies. | 1985 | 3894683 |
| regulation of proteinase activity in aspergillus nidulans. | 1985 | 3894105 | |
| transformation of aspergillus niger by the amds gene of aspergillus nidulans. | aspergillus niger grows poorly on acetamide as a nitrogen or carbon source and lacks sequences detectably homologous to the amds gene encoding the acetamidase of aspergillus nidulans. we have taken advantage of these observations to develop a transformation system for a. niger using the amds gene as a dominant heterologous marker for selecting transformants on the basis of acetamide utilization. transformants varied in their ability to grow on amide media and the number of integrated copies of t ... | 1985 | 3894007 |
| on the genotoxicity of the pesticides endodan and kilacar in 6 different test systems. | two pesticides, the fungicide endodan (ethylene thiuram monosulphide) and the insecticide-acaricide kilacar (bis(parachlorophenyl)cyclopropyl methanol), produced or used in the neighbouring countries of bulgaria and greece were investigated in a coordinated research programme for their genotoxic effects in a variety of test systems. this included the ames test, aspergillus nidulans for mitotic segregation, in vitro human lymphocyte cell cultures for sce and chromosomal aberrations, in vivo bone ... | 1985 | 3892282 |
| spontaneous ir duplications generated at mitosis in aspergillus nidulans: further evidence of a preferential site of transposed attachment. | a radiation-induced translocation, t(iir----iiil), has been shown to be nonreciprocal and to have most of iir, including its terminus, attached uninverted to the terminus of iiil.--progeny with the iir segment in duplicate, obtained from crosses of t(iir----iiil) to strains with a standard genome, were unstable at mitosis; like earlier duplication strains, they suffered deletions from either duplicate segment. frequent mitotic crossing over occurred between the duplicate iir segments so that, fo ... | 1985 | 3891510 |
| cloning an aspergillus nidulans developmental gene by transformation. | we have developed a transformation system for aspergillus nidulans giving a frequency of transformation high enough to screen a gene bank from which we were able to isolate and clone the a. nidulans developmental gene brla by visual selection. the vector contains the selective marker argb+, and with it a frequency of transformation of 500 stable transformants/micrograms plasmid dna can regularly be achieved. the evidence suggests that transformation is by integration but spontaneous excision of ... | 1985 | 3891328 |
| sexual and asexual reproduction of aspergillus nidulans in vivo. | 1985 | 3887151 | |
| paramorphogenic and genotoxic activity of triton x-100 and sodium dodecyl sulphate in aspergillus nidulans. | the genotoxic activities of triton x-100 and sodium dodecyl sulphate in aspergillus nidulans were assessed in order to evaluate their relative merits as paramorphogenic agents. triton x-100 was found to be ideally suited to this purpose due to its efficient paramorphogenic effect and lack of genotoxicity. sodium dodecyl sulphate was considered unsuitable since it reduced viability and was inconsistent in its paramorphogenic action. | 1985 | 3885021 |
| the sterols of growth and stationary phases of aspergillus nidulans cultures. | the accumulation of 4-desmethyl and 4,4-dimethyl sterols, as well as the triterpenoid beta-amyrin, was analysed during both exponential and stationary phases of aspergillus nidulans growth. throughout growth, the amount of 4-desmethyl sterol was proportional to the cellular dry weight, while the dimethyl sterols and beta-amyrin stopped accumulating after day 2. the sterols were found primarily as the free alcohol and not as fatty acid esters, the glycosides, or acyl glycosides. the amount of bet ... | 1985 | 3884732 |
| mutagenicity of trichloroethylene, trichloroethanol and chloral hydrate in aspergillus nidulans. | a trichloroethylene (tce) sample, free of epoxides, has been assayed for its ability to induce gene mutations (methionine suppressors) and mitotic segregation in the mould aspergillus nidulans. no increase in the spontaneous frequency of methionine suppressors was observed when conidia of a haploid strain were plated on selective medium and exposed to tce vapours. a weak but statistically significant increase in methionine suppressors was detected, however, when conidia of cultures grown and con ... | 1985 | 3883153 |
| 13c nmr studies of carbon metabolism in the hyphal fungus aspergillus nidulans. | natural-abundance high-resolution 13c nmr spectra (linewidth, 10 hz) of the hyphal fungus aspergillus nidulans have been obtained after growth on glycolytic or gluconeogenic carbon sources. various polyols, some tricarboxylic acid-cycle intermediates and amino acids, and some phospholipids and fatty acyl compounds are present. the polyols found are mannitol, arabitol, erythritol, and glycerol. the nature of the carbon source has a pronounced effect on the pool sizes of the various polyols. all a ... | 1985 | 3881752 |
| a case of guttural pouch mycosis in a horse. | a case of guttural pouch mycosis in an 11-year-old horse is described. the fungus isolated was identified as emericella nidulans. housing under bad hygienic conditions without ventilation for three years might have been a predisposing factor. | 1986 | 3725585 |
| genotoxicity of hexachlorobenzene and other chlorinated benzenes. | hexachlorobenzene (hcb) and other chlorinated benzenes have had only limited mutagenicity evaluation but tests have shown a general lack of evidence for mutagenicity. bacterial reverse mutation studies have been typically negative and only a few studies on cultured mammalian cells have been published. recent reports indicate that hcb does not induce sister chromatid exchange or dna-strand breakage in vitro. a single report of hcb-induced reverse mutation in yeast is suspect due to the failure to ... | 1986 | 3596730 |
| the nature of l8 and l8s8 forms of ribulose bisphosphate carboxylase/oxygenase from chromatium vinosum. | l8 and l8s8 forms of ribulose bisphosphate carboxylase/oxygenase (rubisco) have been prepared from chromatium vinosum by the extremely mild method of centrifugal fractionation. only the l8s8 form is detectable in crude extracts of this organism. both forms show immunological identify in double diffusion studies using antibody to l subunits of the l8s8 form. l subunits from both l8 and l8s8 enzymes are identical by the criteria of peptides observed after limited proteolysis and n-terminal sequenc ... | 1987 | 3579306 |
| use of alpha- and beta-tubulin mutants for the study of spontaneous and induced chromosomal mis-distribution in aspergillus nidulans. | the effect of two different mutations, one involving an alpha-tubulin (tuba) and the other a beta-tubulin (bena33) gene, on somatic segregation has been investigated in diploid strains of a. nidulans. both mutations, particularly bena33, increase the level of spontaneous chromosomal mis-distribution (cmd) phenomena, without affecting the frequency of crossing-over. the employment of homozygous strains for each of the two mutations in sensitivity tests toward various chemicals, allowed the clear ... | 1987 | 3574324 |
| biosynthesis of thiamin. different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms. | the nitrogen atom of glycine was incorporated into the thiazole moiety of thiamin in the aerobic microorganisms bacillus subtilis, pseudomonas putida, saccharomyces cerevisiae, mucor racemosus, neurospora crassa, and emericella nidulans. it was not incorporated in the case of the facultative anaerobic microorganisms escherichia coli and enterobacter aerogenes, which, however, did incorporate the nitrogen atom of tyrosine. these results show that aerobic microorganisms and facultative anaerobic m ... | 1987 | 3566774 |
| mms sensitivity of all amino acid-requiring mutants in aspergillus and its suppression by mutations in a single gene. | all available amino acid-requiring mutants of aspergillus nidulans were found to be hypersensitive to mms (methyl methanesulfonate) to various degrees. on mms media, secondary mutations could be selected which suppress this mms sensitivity but do not affect the requirement. many such mutations were analyzed and found to be alleles of one gene, smsa (= suppressor of mms sensitivity), which mapped distal on the right arm of chromosome v. this gene is more likely to be involved in general regulatio ... | 1987 | 3556318 |