Publications

accumulation and loss of lindane in the muscles, liver and brain of some freshwater fish after intoxication with gamacarbatox suspension.	experiments were conducted under laboratory conditions on young specimens of carassius carassius l., cyprinus carpio l. and on adult specimens of leucaspius delineatus haeckel. the fish were subjected to a 30-day exposure to gamacarbatox suspension in a dose of 0.5 mg 1-1, containing 10% of lindane as active substance. directly after the experiment on day 30, and then 40 and 50, lindane content was assessed in the muscles, liver and brain using the method of gas chromatography with an electron c ...	2002	12533964
nasda aquatic animal experiment facilities for space shuttle and iss.	national space development agency of japan (nasda) has developed aquatic animal experiment facilities for nasa space shuttle use. vestibular function experiment unit (vfeu) was firstly designed and developed for physiological research using carp in spacelab-j (sl-j, sts-47) mission. it was modified as aquatic animal experiment unit (aaeu) to accommodate small aquatic animals, such as medaka and newt, for second international microgravity laboratory (iml-2, sts-65) mission. then, vfeu was improve ...	2002	12530375
[genetic efficacy of low doses of ionizing radiation in chronically-irradiated small mammals].	earlier we have established the genetic effects of low dose chronic irradiation in bank vole (somatic and germ cells, embryos), in pond carp (fertilized eggs, embryos, fry) and in laboratory mice (somatic and germ cells) in the range of doses from near-background to 10 cgy. these low dose effects observed in mammals and fish are not expected from extrapolation of high dose experiments. for understanding reasons this discrepancy the comparative analysis of genetic efficiency of low dose chronic i ...	2002	12530144
isolation and characterisation of tilapia beta-actin promoter and comparison of its activity with carp beta-actin promoter.	the regulatory sequence including proximal promoter, untranslated exon 1 and intron 1 of the beta-actin gene from tilapia (oreochromis niloticus) has been isolated and spliced to a beta-galactosidase reporter gene to test its activity. comparisons of promoter activity have been carried out with three different constructs: (1) 1.6 kb tilapia beta-actin regulatory sequence, (2) 1.5 kb carp beta-actin regulatory sequence, and (3) 4.7 kb carp beta-actin regulatory sequence. although the 1.6 kb tilap ...	2003	12527420
fish tissue quality in the lower mississippi river and health risks from fish consumption.	between 1990 and 1994, samples of three shellfish species (i.e. blue crab, callinectes sapidus;crayfish, procambarus acutis; and river shrimp, macrobrachium ohionii) and 16 fish species and were collected at six sites along the lower mississippi river by the louisiana department of environmental quality, office of water resources in coordination with the us environmental protection agency. the fish species included: bigmouth buffalo (ictiobus cyanellus); blue catfish (ictalurus furcatus); carp ( ...	2003	12526903
cardiac ankyrin repeat protein (carp) expression in human and murine atherosclerotic lesions: activin induces carp in smooth muscle cells.	cardiac ankyrin repeat protein (carp) is a transcription factor-related protein that has been studied most extensively in the heart. in the present study, we investigated the expression and the potential function of carp in human and murine atherosclerosis.	2003	12524226
toxicity of cypermethrin in labeo rohita fingerlings: biochemical, enzymatic and haematological consequences.	the effect of exposure to sub-lethal concentrations of cypermethrin, a synthetic pyrethroid pesticide, on biochemical parameters of muscle, blood and enzyme activities in brain, liver and kidney of the indian major carp, labeo rohita was studied. the sub-lethal exposure studies were done for up to 45 days at 1/10 and 1/50 of 96 h lc(50) of cypermethrin. the 96 h lc(50) was found to be 0.139 ppm. rna levels decreased while dna levels were elevated. acid phosphatase was unchanged while alkaline ph ...	2003	12524023
[estimation of the allergenicity of specialized canned baby food made from fish, vegetables, and grains].	the influence of consumption of rations containing canned baby foods from mirror carp and black sea mullet on severity of system anaphylactic reaction to model food allergen in rats was studied and the level of antibodies to proteins of black sea mullet, mirror carp, alaska pollack, trout and big head was determined in serum of infants, suffering from food allergy through immune-enzyme analysis.	2002	12522951
relationship between the changes in cellular volume of fish spermatozoa and their cryoresistance.	we have investigated the hypothesis that the spermatozoa of marine fish are more resistant than freshwater species to the dynamic changes in osmotic pressure that occur during the process of cryopreservation. we show that while the spermatozoa of marine fish can be successfully activated across a wide range of osmotic pressures (0-2000 mosmol/l), those of the freshwater species only survive activation within a more restricted range (0-300 mosm/l). after freeze-thawing, up to 30 percent of motile ...	2015	12522505
[studies of the projection for carpometacarpal joint of the thumb that considered patient burden].	the carpometacarpal joint of the thumb is the most important in manual exercise function. robert method is used for the projection for carp metacarpal joint of the thumb broadly. but, the physique is difficult when there is a pain to a person of advanced age and an upper drumstick in robert method. so, we devised the photography method that reduced patient burden. comparison examined robert method and tokai method. detect ability is equal in both method. patient burden is reduced in tokai method ...	2002	12522347
effects of phase iii cardiac rehabilitation programs on health-related quality of life in elderly patients with coronary artery disease: juntendo cardiac rehabilitation program (j-carp).	the purpose of this prospective randomized controlled trial was to assess the impact of phase iii comprehensive cardiac rehabilitation (cr) on health-related quality of life (hrqol) in elderly patients with coronary artery disease (cad). thirty-eight elderly males (mean age, 70 years) with cad were stratified as the intervention group (n=20) and the control group (n=18). in the intervention group, patients participated in cr for 6 months, whereas in the control group, they received standard care ...	2003	12520156
structure, activity, and distribution of fish osteocalcin.	osteocalcin (bone gla protein) is an extracellular matrix protein synthesized by osteoblasts that is a marker of bone. osteocalcin probably originated in the ancestors of teleostei or bony fish and of the tetrapoda or amphibians, reptiles, birds, and mammals. we have characterized the cyprinus carpio (carp) osteocalcin for mineral binding to hydroxyapatite, amino acid sequence, and extent of secondary structure. hydroxyapatite binding is enhanced in the presence of calcium. the alpha-helical con ...	2003	12517753
a contribution to the investigation by light and electron microscope of langerhans islets of the carp (cyprinus carpio l.).		1968	12512099
water quality, biofilm production and growth of fringe-lipped carp (labeo fimbriatus) in tanks provided with two solid substrates.	the effects of two substrates, sugarcane bagasse (t(1)) and paddy straw (t(2)) on water quality and growth of labeo fimbriatus were studied in mud-bottomed, manured cement tanks, in triplicate; a set of three tanks without substrate served as control (t(3)). addition of manure and substrate brought about a decrease in dissolved oxygen level, but it stabilized after 15 days, when the tanks were stocked with 30 fish each, fed at 3% body weight daily and reared for 90 days. total ammonia content in ...	2003	12507865
cyclin a2 is differentially expressed during oocyte maturation between gynogenetic silver crucian carp and gonochoristic color crucian carp.	silver crucian carp (carassius auratus gibelio) is a unique gynogenetic fish. because of its specific genetic background and reproduction mode, it is an intriguing model system for understanding regulatory mechanism of oocyte maturation division. it keeps its chromosomal integrity by inhibiting the first meiotic division (no extrusion of the first pole body). the spindle behavior during oocyte maturation is significantly different from that in gonochoristic fish. the chromosomes are first arrang ...	2003	12506399
megalocornea-mental retardation syndrome: an additional case report.	we report here on a japanese male infant with megalocornea-mental retardation (mmr) syndrome. he had megalocornea (corneal diameter: 13 mm) without glaucoma, developmental retardation, hypotonia, frontal bossing, high-arched palate, carp-like mouth, micrognathia, and delayed myelination. he seems to be included in verloes type of the mmr syndrome.	1997	12503196
classification and characterization of lactic acid bacteria isolated from the intestines of common carp and freshwater prawns.	fifty-four isolates of lactic acid bacteria were obtained from the intestines of the common carp (cyprinus carpio) and freshwater prawns (macrobrachium rosenbergii) in nakorn-pathom province, thailand. all isolates were gram-positive and catalase-negative cocci that did not produce gas from glucose and formed dl or l(+) lactic acid only. most isolates were able to grow in broth at ph 9.6, in 6.5% nacl (w/v) and 40% (w/v) bile. these isolates were divided into six groups (a-f) by sugar fermentati ...	1999	12501375
carbonic anhydrase inhibitors.	at least 14 different carbonic anhydrase (ca, ec 4.2.1.1) isoforms were isolated in higher vertebrates, where these zinc enzymes play crucial physiological roles. some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), others are membrane-bound (ca iv, ca ix, ca xii, and ca xiv), ca v is mitochondrial and ca vi is secreted in saliva. three acatalytic forms are also known, which are denominated ca related proteins (carp), carp viii, carp x, and carp xi. several important physiological ...	2003	12500287
haematology and some blood chemical parameters of young carp till the age of three years.	haematological and biochemical analyses of blood were performed in carp (cyprinus carpio l.) kept in small ponds. caught and anaesthetised carp were clinically examined and blood samples were taken at regular intervals during the three years. in the first year of examinations, the haemoglobin and haematocrit values of carp fry significantly increased (p < 0.01) from june to september. the intensive growth of carp in the summer period in the second year was accompanied by adequate erythropoiesis. ...	2002	12498150
ligula intestinalis--a tapeworm contraceptive.	human contraceptives are 'big business', but might the real breakthrough come out from the pharmaceutical industry but from a tapeworm? ligula intestinalis can induce infertility in infected fish of the carp family - both males and females. if the mechanism for this can be discovered, this humble flatworm could drastically change contraceptive practices with one pill for all.	2002	12486303
a comparison of trace element concentrations in cultured and wild carp (cyprinus carpio) of lake kasumigaura, japan.	the concentrations of 13 elements were determined in the muscle, liver, intestine, kidney, and gonads of cultured and wild carp caught at two sites in lake kasumigaura, japan, between september 1994 and september 1995. despite having a reputation for being heavily polluted, the carp were not heavily burdened with metals. our results suggest that despite their dietary differences, the wild and cultured fish were accumulating and distributing metals in the same manner and that aquaculture practice ...	2002	12485577
the combined use of chemical and biochemical markers to assess water quality in two low-stream rivers (ne spain).	carps (cyprinus carpio) and red swamp crayfish (procambarus clarkii) were sampled from two lowstream mediterranean rivers (anoia and cardener) receiving extensive urban and industrial waste water discharges. tissue residues of selected pollutants (organochlorinated compounds, polycyclic aromatic hydrocarbons (pahs)) were determined in conjunction with different biochemical responses (cytochrome p450, phase ii enzymes) with the aim of investigating whether resident organisms were responsive to ch ...	2002	12483808
chromium induced alterations in some biochemical profiles of the indian major carp, labeo rohita (hamilton).		2003	12478433
molecular and functional characterization of carp tnf: a link between tnf polymorphism and trypanotolerance?	two carp tumor necrosis factor alpha (tnfalpha) genes have been cloned and sequenced. both tnf1 and tnf2 sequences have several polymorphisms in the 3' utr and tnf2 has a polymorphism in the coding sequence. lipopolysaccharide and the protozoan blood flagellate trypanoplasma borreli induced expression of tnfalpha in carp head kidney phagocytes when added in vitro. differential expression was observed, with tnf2 being higher expressed than tnf1. we used the tnfalpha-specific inhibitor pentoxifyll ...	2003	12477499
suppression by zinc of transient off responses of carp amacrine cells to red light is mediated by gaba(a) receptors.	modulation by zn(2+) of on and off responses of transient amacrine cells driven by red- and green-sensitive cones was investigated in isolated, superfused carp retina, using intracellular recording techniques. zn(2+) selectively abolished the off response to red flash of the transient amacrine cells. this zn(2+) effect was mimicked by gaba application and was blocked by bicuculline, indicating the involvement of gaba(a) receptors. such differential modulation was observable neither in bipolar ce ...	2002	12468048
purification of two types of gonadotropin receptors from carp ovarian follicles: overlapping recognition by two different ligands.	teleostean gonadotropin receptors were solubilized from the plasma membrane preparation of carp ovarian follicles by lithium diiodosalicylate and triton x-100. solubilization resulted fourfold increase in gth binding activity as compared to the crude plasma membrane preparation. an addition of 25% glycerol and protease inhibitors to the solubilized receptor retained more than 90% original activity at -20 degrees c for 30 days. gel filtration through sephadex g 100 significantly increased the spe ...	2002	12460599
a new method for fish leucocyte counting and partial differentiation by flow cytometry.	a simple and rapid method for analysis of fish blood cells is presented. carp (cyprinus carpio) blood was diluted 200 times with hanks' solution containing 1 microg/ml of dioc6(3) which is a fluorescent, lipophilic dye. after staining for 10 min, the blood cells were measured by a flow cytometer (facs). several blood cell populations were identified by different fl-1 (green fluorescence), fsc (forward scatter), and ssc (side scatter) properties. fl-1 v. ssc or fsc v. ssc dot-plot of stained bloo ...	2002	12458744
regulation of growth hormone release in common carp pituitary cells by pituitary adenylate cyclase-activating polypeptide: signal transduction involves camp- and calcium-dependent mechanisms.	pituitary adenylate cyclase-activating polypeptide (pacap) is a member of the glucagon/secretin peptide family and its molecular structure is highly conserved among vertebrates. in this study, the role of pacap in regulating growth hormone (gh) secretion in fish was examined in vitro using common carp pituitary cells under column perifusion. a dose-dependent increase in gh release was observed after exposing pituitary cells to increasing doses of ovine pacap38 (opacap38) and pacap27 (opacap27), ...	2002	12457043
phenotypic characteristics and pathogenicity of aeromonas genomospecies isolated from common carp (cyprinus carpio l.).	to evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile aeromonas strains.	2002	12452960
different effects of low ca2+ on signal transmission from rods and cones to bipolar cells in carp retina.	modulation of signal transmission from rods, red-sensitive (r-) and green-sensitive (g-) cones to bipolar cells by lowering extracellular ca(2+) was studied in the isolated superfused carp retina using intracellular recording techniques. low ca(2+) (nominally ca(2+)-free) potentiated light responses of rod dominant on bipolar cells (rod-on-bcs). on the other hand, responses of cone dominant on bipolar cells (cone-on-bcs) driven by g-cones were dramatically decreased whereas those driven by r-con ...	2002	12443989
endocrine effects of sublethal exposure to persistent organic pollutants (pops) on silver carp (hypophthalmichthys molitrix).	silver carp (hypophthalmichthys molitrix) samples were collected from five selected sites that represent diverse levels of downgraded persistent organic pollutants (pops) contamination in ya-er lake in october 1999. hepatic ethoxyresorufin-o-deethylase (erod) and udp glucuronosyltransferase (udpgt) activities, hepatosomatic index (hsi), hepatic retinoids, serum thyroid hormones were measured. it was found that hepatic retinol and serum free 3,5,3'- tetraiodothyronine (ft3) significantly increase ...	2002	12442791
reproductive medicine in koi (cyprinus carpio).	each year more veterinarians are seeing ornamental fish patients and our knowledge of how to manage the pet fish patient is increasing at a rapid pace. a large percentage of these patients are nishikigoi (cyprinus carpio) which are taxonomically an ornamental carp. in this country and in many parts of the world we refer to these fish as koi. reproductive medicine problems are well documented in koi and a thorough knowledge of this subject will aid the fish practitioner in diagnosis and treatment ...	2002	12442718
the role of desaturases in cold-induced lipid restructuring.	all organisms respond to environmental challenge by adaptive responses, although, in many cases, the underlying molecular mechanisms are not understood. in the case of membranes, the physical structure of membrane phospholipids is conserved in the face of cold, rigidifying conditions by the elevated proportions of unsaturated fatty acids. we have observed a clear positional specificity in this substitution and head group preferences in carp liver membranes. we have also demonstrated changes in t ...	2002	12440978
structural dependencies of h3jnc' scalar coupling in protein h-bond chains.	the h-bond ((h3)j(nc')) and peptide bond ((1)j(nc')) scalar couplings establish connectivity of the electronic structure in the h-bond chains of proteins. the correlated changes of (h3)j(nc') and (1)j(nc') couplings extend over several peptide groups in the chains. consequently, the electronic structure of the h-bond chains can affect (h3)j(nc') in a manner that is independent of the local h-bond geometry. by taking this into account, and by using a more complete set of h-bond geometry parameter ...	2002	12440921
electrospray ionisation mass spectrometric analysis of lipid restructuring in the carp (cyprinus carpio l.) during cold acclimation.	cold acclimation of carp from 30 degrees c to 10 degrees c causes a restructuring of liver microsomal phospholipids characterised by increased proportions of monounsaturated fatty acid in phosphatidylcholine (pc) and phosphatidylethanolamine (pe). here, we have used electrospray ionisation mass spectrometry (esi-ms) to determine the patterns of alteration to individual molecular species compositions of pc, pe and phosphatidylinositol (pi) in response to gradually decreasing temperature. the resu ...	2002	12432020
stress adaptation, cortisol and pubertal development in the male common carp, cyprinus carpio.	this paper reviews a series of recent studies on the effect of adaptation to chronic stress on pubertal development in the common carp. in pre-pubertal male common carp adaptation to temperature stress caused a retardation of testicular development. stress-induced delay of the first wave of spermatogenesis could be prevented by treatment with a cortisol antagonist, indicating that the stress effect is mediated by cortisol. chronically elevated cortisol levels affected all parts of the brain-pitu ...	2002	12431803
comparisons of pbde composition and concentration in fish collected from the detroit river, mi and des plaines river, il.	polybrominated diphenyl ethers (pbdes) were identified in fish collected from the detroit river, mi and des plaines rivers, il. in the detroit river fish, carp and large mouth bass, the congener patterns were dominated by the 2,2',4,4'-tetrabromo (bde-47) congener, however, in des plaines river carp the dominant isomers were the heptabromo congeners bde-181 and bde-183 and lesser amounts of another heptabromo congener, bde-190, and two hexabromo congeners, bde-154 and bde-153. three possible sou ...	2002	12431009
nitric oxide controls the light adaptive chromatic difference in receptive field size of h1 horizontal cell network in carp retina.	in carp retina, the receptive field size of the h1-type horizontal cell (hc) network is known to be chromatically selective, as electrophysiological signals are generated by short-wavelength (sw) light stimuli, which spread much less than those for long-wavelength (lw). we have shown previously that the signalling mechanism underlying this chromatic difference operates only in the light-adapted retina and that it involves cgmp as an intermediary messenger. in the present study, the possible role ...	2002	12428137
utilization of fermented silkworm pupae silage in feed for carps.	fermented silkworm pupae (swp) silage or untreated fresh swp pastes were incorporated in carp feed formulations replacing fishmeal. the feed formulations were isonitrogenous (30.2-30.9% protein) and isocaloric (me = 2905-2935 kcal/kg). feeding under a polyculture system consisting of 30% each of catla (catla catla), mrigal (cirrhinus mrigala) and rohu (labeo rohita) with 10% silver carps (hypophthalmychthys molitrix) was carried out in ponds to evaluate the nutritive quality of swp silage. survi ...	2003	12421005
potential of amaranthus seeds in supplementary feed and its impact on growth in some carps.	amaranthus seeds were used at three different levels (20%, 35%, 50%) in fish diets under a semi-intensive fish culture system and their impact on the growth of common carp, cyprinus carpio, and rohu, labeo rohita, was studied. growth in terms of body weight gain was maximum in fish fed on diets containing 20% amaranthus seeds, that replaced rice bran and groundnut oil cake in the feed. overall, the fish fed on diets containing amaranthus seeds at different levels showed better growth than the co ...	2003	12421004
gonadosomatic index and testis morphology of common carp (cyprinus carpio) in rivers contaminated with estrogenic chemicals.	to study the effect of estrogenic chemicals on fish, the gonadosomatic index (gsi = [testis weight/body weight] x 100) and testis histology of mature common carp (cyprinus carpio) from 2 contaminated sites (ishizu and wada rivers, osaka) and a control site were examined between june 1998 and march 2001. the concentration of nonylphenol, bisphenol a and 17beta-estradiol in the ishizu river was 3-4 times higher than in the wada river. in the pre-breeding and breeding seasons, there were no signifi ...	2002	12419869
[effects of pituitary adenylate cyclase activating polypeptide (pacap) on intracellular camp and ca(2+) levels in common carp (cyprinus carpio) pituitary cells in vitro].	in order to study signal transduction in pacap-stimulated gh release, effects of ovine pacaps (opacaps) on camp synthesis and intracellular free ca(2+)([ca(2+)](i)) levels in primarily cultured common carp (cyprinus carpio) pituitary cells were investigated using radioimmunoassay (ria) for camp and [ca(2+)](i) indo-1/am loading techniques. the main results obtained could be briefly summarized as follows: parallel with their gh-releasing effects, opacap-38 (0.01 nmol/l to 1 micromol/l) and opacap ...	2002	12417926
myosin heavy chain expression in cranial, pectoral fin, and tail muscle regions of zebrafish embryos.	to investigate whether different myosin heavy chain (mhc) isoforms may constitute myofibrils in the trunk and tail musculature and if their respective expression may be regulated by spadetail (spt) and no tail (brachyury), we identified and characterized mrna expression patterns of an embryonic- and tail muscle-specific mhc gene (named myhz2) during zebrafish development in wild type, spt, and ntl mutant embryos. the identified myhz2 mhc gene encodes a polypeptide containing 1,935 amino acids. d ...	2002	12412043
specific binding of the endocytosis tracer horseradish peroxidase to intestinal fatty acid-binding protein (i-fabp) in apical membranes of carp enterocytes.	in a previous study we had demonstrated that a 15-kda protein present in carp intestinal brush-border membrane vesicles (bbmv) was able to bind the endocytosis tracer horseradish peroxidase (hrp) with high specificity. here we show that this protein corresponds to a peripheral membrane protein, identified by partial amino acid sequence analysis as the intestinal fatty acid-binding protein (i-fabp), a member of the small cytosolic fatty acid binding protein family (fabps). the presence of i-fabp ...	2002	12410603
measurement of the activity of multixenobiotic resistance mechanism in the common carp cyprinus carpio.	the multixenobiotic resistance (mxr) mechanism, mediated by activity of the transmembrane p-glycoprotein, represents a basic biological defence system in aquatic organisms. here we investigate the mxr transport activity in an aquatic vertebrate, the common carp (cyprinus carpio). we measured the accumulation rate of a model mxr substrate, the fluorescent dye rhodamine b (rb), in gills, lateral muscles, liver and bile. results obtained using this method showed a significant increase of rb accumul ...	2015	12408600
granulocyte responses to experimental injection of live and formalin-killed bacteria in carp (cyprinus carpio).	the objective of the study was to investigate the dynamics of changes in number of granulocytes in bacterial infections of carp (cyprinus carpio). carp were inoculated with non-pathogenic or pathogenic bacteria and changes in type i (neutrophils) and type ii granulocyte (basophils/eosinophils) counts in kidney, circulating blood and peritoneal cavity were assessed. after the injection of non-pathogenic bacteria (escherichia coli), the number of type i and ii cells in blood increased after 6-12h, ...	2002	12406659
gluconeogenic enzyme gene expression is decreased by dietary carbohydrates in common carp (cyprinus carpio) and gilthead seabream (sparus aurata).	our objective is to understand the low metabolic utilization of dietary carbohydrates in fish. we compared the regulation of gluconeogenic enzymes at a molecular level in two fish species, the common carp (cyprinus carpio) and gilthead seabream (sparus aurata), known to be relatively tolerant to dietary carbohydrates. after cloning of partial cdna sequences for three key gluconeogenic enzymes (glucose-6-phosphatase (g6pase), fructose biphosphatase (fbpase) and phosphoenolpyruvate carboxykinase ( ...	2002	12401217
characterisation of a monoclonal antibody to carp il-1beta and the development of a sensitive capture elisa.	a carp il-1beta gene was identified from a subtraction hybridisation technology based cdna library from activated carp leucocytes. this gene was cloned into pqe vector carrying 6xhis tag and the protein was expressed. recombinant il-1beta was used to produce hybridomas specific for carp il-1beta. monoclonal antibodies were purified by affinity column and a sandwich elisa for il-1beta was developed with a detection limit of 10 ng of the recombinant protein. using the capture elisa, the presence o ...	2002	12400859
high vitamin a content in some small indigenous fish species in bangladesh: perspectives for food-based strategies to reduce vitamin a deficiency.	recognising the importance of fish in the bangladeshi diet, the objective of the present study was to screen commonly consumed fish species for vitamin a content to evaluate the potential of fish as a vitamin a source in food-based strategies to combat vitamin a deficiency. samples of 26 commonly consumed fish species and one crustacean were collected in kishoreganj and mymensingh, bangladesh. to obtain edible parts, the fish were cleaned by bangladeshi women according to traditional practices. ...	2002	12396468
[patterns of intraspecific variability in carp fishes of the dace subfamily: ecological factors and pathways of phenotypic transformation].	the inverstigation of geographical and ecological variations of wide-spread species of daces allows to reveal regular patterns of phenotypic transformations with limited number of their possible ways. the set of characters involved and the directions of their changes are specific for both examined taxa and putative factors of subspeciation. two basic groups of evolutionary and ecological situations leading to the intensive intraspecific divergence are considered: living or spawning in brackish w ...	2010	12395751
glandular kallikreins in the teleost cyprinus carpio: tissue distribution, possible involvement in prolactin processing, and effect of 17 beta-estradiol in vivo.	we examined glandular kallikrein (gk), a putative prolactin processing protease, in the teleost cyprinus carpio. when employing an anti-centropristis striata gk antibody proteins of 39 kda in muscle, 52 kda in gill, 52 kda in kidney, and two proteins of 46 and 72 kda in pituitary gland were detected. immunoreactive kallikreins were recognized in intermuscle cell tissue, epithelial gill cells, apical region of tubular cells, and prolactin producing lactotrophs in pituitary gland, suggesting a osm ...	2002	12392686
seasonal changes in plasma steroid levels in indian major carp labeo rohita: influence of homologous pituitary extract on steroid production and development of oocyte maturational competence.	circannual variations in plasma levels of testosterone (t), 17beta-estradiol (e(2)), and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (dhp) as well as seasonal fluctuations in ovarian steroid synthetic potential were observed in indian major carp, labeo rohita. a study was also conducted to examine the mechanism of the development of gonadotropin-induced maturational competence in oocytes of this fish. the present study recorded the lowest values of plasma e(2) and t in l. rohita during the perio ...	2002	12392685
cloning and sequencing of wee1 from the ovary of the gynogenetic japanese silver crucian carp (ginbuna) [carassius auratus langsdorfi].	wee1 is one of the genes which regulates the cell cycle. cdna encoding a wee1-homolog has been isolated from japanese silver crucian carp (ginbuna) by using polymerase chain reaction (pcr) techniques. the cdna obtained from the ovary tissue encoded a protein of 526 amino acids and the deduced amino acid sequence showed high homology (69.9%) with xenopus laevis wee1 in the protein kinase domain. a phylogenetic analysis among wee1 amino acid sequences from various organisms showed that the crucian ...	2002	12391727
cloning and sequences of beta-actin genes from rhodeus notatus and the silver carp hypophthalmichthys molitrix (cyprinidae) and the phylogeny of cyprinid fishes inferred from beta-actin genes.	complete beta-actin genes from rhodeus notatus and hypophthalmichthys molitrix (silver carp) were amplified by polymerase chain reaction and cloned into pcrii vector for further characterization. the beta-actin genes of these two fishes were 1718 and 1755 bp in sequence length, respectively, and both of them contained 5 exons and 4 introns. the exon and intron boundaries were identified with the gt/ag rule. the phylogenetic implications of beta-actin gene in the family cyprinidae were shortly di ...	2002	12391726
in vitro assessment of potential mechanism-specific effects of polybrominated diphenyl ethers.	this study examined the ability of environmentally relevant concentrations of 10 different polybrominated diphenyl ethers (pbdes) to induce aryl hydrocarbon receptor (ahr)- and estrogen receptor (er)-mediated gene expression in vitro. it also examined the ability of pbdes to displace steroid hormones from serum proteins in vitro. at concentrations ranging up to 880 ng/ ml, none of the pbdes significantly displaced tritiated 17beta-estradiol (e2) or testosterone from hormone-stripped carp serum. ...	2002	12389923
differential expression of cold- and diet-specific genes encoding two carp liver delta 9-acyl-coa desaturase isoforms.	carp respond to cold by the upregulated expression of delta9-acyl-coa desaturase. here we report the cloning and characterization of cds2, a second delta9-acyl coa-desaturase expressed in carp liver. both cds1 and cds2 complemented the ole1 mutation in saccharomyces cerevisiae, permitting the synthesis of delta9-monounsaturates, confirming their identity as delta9-desaturases. we demonstrate that under a standard feeding regime it is the cds2, and not cds1, transcript that is transiently upregul ...	2003	12388470
changes of carp fof1-atpase in association with temperature acclimation.	previously we have shown, using two-dimensional electrophoresis, that mitochondrial atp synthase (f(o)f(1)-atpase) beta-subunit is the 55-kda protein increased in cold-acclimated carp cyprinus carpio (kikuchi k, itoi s, and watabe s. fisheries sci 65: 629-636, 1999). to clarify the coordinate expression in various subunits of carp f(o)f(1)-atpase with temperature acclimation, we examined the differences in mrna levels of mitochondrial proteins encoded by both nuclear and mitochondrial genes in f ...	2003	12388464
alpha(1)-adrenergic activation of the cardiac ankyrin repeat protein gene in cardiac myocytes.	cardiac ankyrin repeat protein (carp) is a nuclear transcription cofactor that is activated by multiple signaling pathways in hypertrophic cardiac myocytes. since carp has been reported to be a transcriptional co-repressor, its activation during hypertrophy might contribute to the deregulation of gene expression leading to heart failure. here, we found that alpha(1)-adrenergic signaling activates carp mrna expression in rat cardiac myocytes. to examine how alpha(1)-adrenergic signaling activates ...	2002	12384280
antioxidant response modulated by copper in healthy or parasitized carp (cyprinus carpio l.) by ptychobothrium sp. (cestoda).	an increased antioxidant response (catalase, glutathione s-transferase (gst) and glutathione reductase (grd) activities in liver and gst activity in head kidney) was observed in carp parasitized by ptychobothrium sp. compared to healthy fish. in case of a copper contamination of these fish, the decrease in enzymatic activities observed was less pronounced in parasitized than in healthy carp.	2002	12383935
primary structure of myosin heavy chain from fast skeletal muscle of chum salmon oncorhynchus keta.	the nucleotide sequence of the cdna encoding myosin heavy chain of chum salmon oncorhynchus keta fast skeletal muscle was determined. the sequence consists of 5,994 bp, including 5,814 bp of translated region deducing an amino acid sequence of 1,937 residues. the deduced sequence showed 79% homology to that of rabbit fast skeletal myosin and 84-87% homology to those of fast skeletal myosins from walleye pollack, white croaker and carp. the putative binding-sites for atp, actin and regulatory lig ...	2002	12381388
relationships between persistent organic chemicals residues and biochemical constituents in fish from a protected area: the french national nature reserve of camargue.	the reserve of biosphere of camargue [french national nature reserve of camargue (nnrc)] is a protected area frequently exposed to natural and anthropogenic environmental alterations. to evaluate potential contamination of fish with lipophilic chemicals-organochlorines (ocs) and polycyclic aromatic hydrocarbons (pahs)-a biological monitoring survey was carried out. metabolic reserve levels were evaluated to select appropriate biological indicators able to be significant biomarkers. in addition, ...	2002	12379424
in vitro antiestrogenic effects of aryl methyl sulfone metabolites of polychlorinated biphenyls and 2,2-bis(4-chlorophenyl)-1,1-dichloroethene on 17beta-estradiol-induced gene expression in several bioassay systems.	methylsulfonyl (meso(2)) metabolites of polychlorinated biphenyls (pcbs) and 2,2-bis(4-chlorophenyl)-1,1-dichloroethene (4,4'-dde), itself a metabolite of the insecticide 4,4'-ddt, are emerging as a major class of contaminants in the tissues of wildlife and humans. we investigated the antiestrogenic capacity and potencies of 3'- and 4'-meso(2)-2,2',4,5,5'-pentachlorobiphenyl (cb101) and -2,2',4,5'-tetrachlorobiphenyl (cb49), which are among the most environmentally persistent meso(2)-pcbs, and 3 ...	2002	12377985
influence of the polyphenolic tannic acid on the toxicity of the insecticide deltametihrin to fish. a comparative study examining both biochemical and cytopathological parameters.	humics and pesticides are present in aquatic environment and the toxicological consequences of their chemical interaction is well studied. however, data concerning the mechanism of the biochemical action of humic-pesticide combinations are scarce, especially in vertebrates. thus we have chosen to study the in vivo effects of the plant polyphenolic tannic acid and the pyrethroid insecticide deltamethrin [decis] alone or in combination on hepatic xenobiotic-metabolizing enzyme activities and the a ...	2002	12371615
differential regulation of the two metallothionein genes in common carp.	the expression of two metallothionein (mt) genes was followed in carp (cyprimus carpio) in vivo during exposure to as and cu. changes in the levels of mt-1 and mt-2 mrna in the liver and kidney were detected by semi-quantitative rt-pcr. the inducibility of the two mt isoforms was tissue- and metal-specific. regardless of whether as or cu was applied, the liver was more responsive than the kidney. copper influenced the expression of both isoforms: the mt-1 and mt-2 mrna levels increased in both t ...	2002	12371614
endocrine disruptors in sewage treatment plants, receiving river waters, and sediments: integration of chemical analysis and biological effects on feral carp.	occurrence of alkylphenol ethoxylates or their metabolites (alkylphenols and carboxylated derivatives), as well as natural and synthetic steroids in sewage treatment plant (stp) effluents and in their receiving waters, has been related to biological effects, measured as alterations of plasma vitellogenin (vtg) concentration in natural fish populations. water composites of stp influents, effluents, sludge, river water, sediment, and feral carps (cyprinus carpio) were analyzed over a seven-month p ...	2002	12371491
efficacy of water hyacinth compost in nursery ponds for larval rearing of indian major carp, labeo rohita.	the use of water hyacinth compost as manure in nursery ponds for larval rearing of indian major carp, labeo rohita was studied. better performance with regard to growth and survivability of the larvae was recorded in the ponds treated with water hyacinth compost (group a) than in either the ponds treated with inorganic fertilizers (group b) or the ponds where no treatment was applied (group c). the average percentages of survival in groups a, b and c were 14.3, 11.2 and 5.0, respectively. thus a ...	2002	12365499
evaluation of the tissue reactions in the skin and body wall of koi (cyprinus carpio) to five suture materials.	five different suture materials (silk, monofilament nylon, polyglyconate, polyglactin 910, and chromic gut) were placed in the skin and body wall of 10 doitsu (scaleless) koi (cyprinus carpio). after seven days the sutures were retrieved from five of the fish in 5 mm and 6 mm punch biopsies, and after 14 days they were retrieved in the same way from the other five. the tissue reactions were evaluated by gross visual inspection and by histological examination. the total inflammatory reaction was ...	2002	12356236
isolation, expression and characterization of carp retinol-binding protein.	vitamin a alcohol and its precursors carotenoids are introduced in the organism with the diet, transported to the liver and from there as retinol to target tissues by a specific carrier, the retinol-binding protein (rbp). rbp, isolated and characterized in many vertebrates, shows very high homology among the species investigated; however, very little is known in fish. in the present work rbp cdna isolated from a carp liver library was transcribed and translated in vitro and the corresponding pro ...	2002	12354658
copper speciation in the gill microenvironment of carp (cyprinus carpio) at various levels of ph.	the fish gill microenvironment of cyprinus carpio under stress of copper exposure was investigated. ph and other parameters including free copper activity, alkalinity, and inorganic and organic carbons in the surrounding water (inspired water) and in the gill microenvironment (expired water) were measured or calculated at various levels of ph and varying total copper concentrations. the chemical equilibrium calculation (from mineqa2) and complexation modeling (mucus-copper) were coupled to calcu ...	2002	12297083
republic act no. 6657, 10 june 1988.	this act institutes the comprehensive agrarian reform program (carp). carp takes as its declared aim "the establishment of owner cultivatorship of economic-size farms as the basis of philippine agriculture" (section 2). it prohibits the retention by landowners of more than five hectares of land and, for each child of the landowner over 15 years old, an additional three hectares, providing he or she is personally cultivating that land (section 6). that limit of three hectares is also the upper ...	1988	12289467
marker enzyme assessment in the liver of cyprinus carpio (l.) exposed to 2,4-d and azinphosmethyl.	the potential utility of antioxidant enzymes and lipid peroxidation as indicators of exposure to 2,4-d and azinphosmethyl together with the toxic effects of these compounds in freshwater fish cyprinus carpio were evaluated. biochemical parameters were recorded spectrophotometrically in fish liver, which were exposed to a single dose of 2,4-d and azinphosmehtyl (1/3 lc(50)), and their mixture at 1:1 ratio for 24, 48, 72, and 96 h. the most sensitive parameter was glutathione s-transferase (gst) a ...	2002	12242687
zn(2+) modulates light responses of color-opponent bipolar and amacrine cells in the carp retina.	the effects of zn(2+) on color-opponent bipolar cells (bcs) and amacrine cells (acs) were studied in the isolated superfused carp retina using intracellular recording techniques. bath-applied zn(2+) (25 micro m) depolarized r(+)g(-)-type bcs and suppressed both depolarizing responses of these cells to red (680 nm) flashes and hyperpolarizing ones to green (500 nm) flashes. following zn(2+) application, g(+)r(-)-type bcs were hyperpolarized, which was accompanied by a potentiation of their depola ...	2002	12242098
suppression in function of phagocytic cells in common carp cyprinus carpio l. injected with estradiol, progesterone or 11-ketotestosterone.	the modulation of phagocytic cells by beta-estradiol, 11-ketotestosterone, progesterone and cortisol was analysed in common carp (cyprinus carpio l.). carp were injected intraperitoneally (1 and 5 microg/kg fish) with each steroid and the activity of kidney macrophages was recorded 1 and 3 days post-injection. bovine serum albumin-injected fish served as controls. phagocytosis, superoxide anion and nitric oxide production were suppressed in all sex steroid treatments, and the suppression was in ...	2002	12223196
the carp muscle-specific sub-isoenzymes of creatine kinase form distinct dimers at different temperatures.	we have previously cloned three muscle-specific sub-isoforms of creatine kinase (ck, ec 2.7.3.2) from the common carp ( cyprinus carpio ), designated m1-ck, m2-ck, and m3-ck. the enzyme has a key role in maintaining the energy homoeostasis of cells with fluctuating energy requirements. in the present paper, we report that all three m-cks in the red and white muscle of different temperature-acclimatized carp were ubiquitously distributed in the cytosol and along membranes. in addition, the expres ...	2002	12213085
distribution of gfap immunoreactive structures in the rhombencephalon of the sterlet (acipenser ruthenus) and its evolutionary implication.	previous studies have revealed that although the brains of cypriniform teleosts (iberian barb, barbus comiza; carp, cyprinus carpio; goldfish, carassius auratus) are rich in glial fibrillary acidic protein (gfap), they have, however, areas devoid of gfap immunoreactivity. the largest ones of these are in the rhombencephalon, e.g., the zones of the sensory and motor neurons in the vagal lobe. our studies in amniotes suggested that the gfap immunonegative areas could be characteristic of the more ...	2002	12210122
a web-accessible complete transcriptome of normal human and dmd muscle.	we present an assessment of the complete transcriptome of human skeletal muscle in duchenne muscular dystrophy patient muscle and non-dystrophic controls (36 rnas analyzed from ten duchenne dystrophy and eight controls; approximately 65,000 gene/expressed sequence tag/probe sets queried on u95 five-genechip series and musclechip). the use of the multiple chip types allowed us to compare results from different probe sets for the same gene: we found excellent concordance between different probe se ...	2002	12206807
cross-linking of zp2 and zp3 by transglutaminase is required for the formation of the outer layer of fertilization envelope of carp egg.	a transglutaminase (tgase) cdna was cloned from carp ovary. it was highly homologous to zebrafish tgase. immunoblot and enzymatical assay showed that tgase was present on the chorion and in the cytoplasm of carp eggs. addition of tgase inhibitor, cadaverine or ethylene diaminetetracetic acid (edta) to the cortical reaction medium impaired the formation of the outer layer of fertilization envelope (fe(o)), the adhesive structure of carp egg. fibroin-like substance (fls), cystatin, cathepsin-like ...	2002	12203834
immunisation of indian major carps against aeromonas hydrophila by intraperitoneal injection.	the indian major carps catla catla, labeo rohita and cirrhinus mrigala were immunised against the potent bacterial fish pathogen, aeromonas hydrophila by the intraperitoneal route, in field conditions. two different polyvalent antigen preparations namely, whole cell and extracellular products (ecp) were used. immunisation schedule consisting of a single booster dose given 28 days after priming resulted in a good agglutinating antibody response in the carps. kinetics of the response were similar ...	2002	12201649
seasonal variation of plasmatic and hepatic vitellogenin and erod activity in carp, cyprinus carpio, in relation to sewage treatment plants.	concern about the health of aquatic fauna living in waters containing biologically active levels of estrogenic compounds is particularly focused on the effects on their reproductive success. to that end, carp, cyprinus carpio, a feral fish living in warm waters of southern europe (ne spain), were selected for signs of estrogenicity. the study area covered two tributaries (the anoia and the cardener) of the llobregat river both known to be polluted by estrogenic compounds. the estrogenicity in th ...	2002	12200088
first record of neoergasilus japonicus (poecilostomatoida: ergasilidae), a parasitic copepod new to the laurentian great lakes.	the parasitic copepod neoergasilus japonicus, native to eastern asia, was first collected from 4 species of fish (fathead minnow, pimephales promelas; largemouth bass, micropterus salmoides; pumpkinseed sunfish, lepomis gibbosus; and yellow perch, perca flavescens) in july 1994 in saginaw bay, lake huron, michigan. further sampling in the bay in 2001 revealed infections on 7 additional species (bluegill, lepomis macrochirus; carp, cyprinus carpio; channel catfish, ictalurus punctatus; goldfish, ...	2002	12197110
effects of a parasite-induced nephritis on osmoregulation in the common carp cyprinus carpio.	carp cyprinus carpio infected with the haemoflagellate trypanoplasma borreli undergo progressive nephritis associated with a destruction of approx. 40% of the nephric tubules. in an attempt to analyse the effect of the nephritis on the osmoregulation of affected carp, the clinical chemical properties of plasma and urine samples were analysed. parasitised carp excreted greater amounts of electrolytes in their urine than uninfected carp which excreted highly diluted urine with an osmolality of abo ...	2002	12180703
a novel functional class i lineage in zebrafish (danio rerio), carp (cyprinus carpio), and large barbus (barbus intermedius) showing an unusual conservation of the peptide binding domains.	species from all major jawed vertebrate taxa possess linked polymorphic class i and ii genes located in an mhc. the bony fish are exceptional with class i and ii genes located on different linkage groups. zebrafish (danio rerio), common carp (cyprinus carpio), and barbus (barbus intermedius) represent highly divergent cyprinid genera. the genera danio and cyprinus diverged 50 million years ago, while cyprinus and barbus separated 30 million years ago. in this study, we report the first complete ...	2002	12165519
morphology and life cycle of apatemon hypseleotris species novum from australia including metacercariae viability and excystment.	experimental infection of pigeon squabs and rats with encysted metacercariae from the western carp gudgeon (hypseleotris klunzingeri) showed them to be infected with a new strigeid trematode, apatemon hypseleotris. growth and development of a. hypseleotris in pigeons were significantly higher than in rats. eggs appeared in pigeon faeces within 7-14 days; miracidia hatched within 15-21 days and in the snail lymnaea tomentosa released within 21 days. cercariae experimentally encysted in the leeche ...	2002	12161969
bioavailability and toxicity of dietborne copper and zinc to fish.	to date, most researchers have used dietborne metal concentrations rather than daily doses to define metal exposure and this has resulted in contradictory data within and between fish species. it has also resulted in the impression that high concentrations of dietborne cu and zn (e.g. > 900 mg kg(-1) dry diet) are relatively non-toxic to fish. we re-analyzed existing data using rations and dietborne metal concentrations and used daily dose, species and life stage to define the toxicity of dietbo ...	2002	12161165
glycosylated molecular variants of c-reactive proteins from the major carp catla catla in fresh and polluted aquatic environments.	elevated level of pollutant specific glycosylated molecular variants of c-reactive protein have been purified to electrophoretic homogeneity from the sera of major carp, catla catla confined in freshwater (crp(n)) and water polluted with nonlethal doses of cadmium (crp(cd)), mercury (crp(hg)), phenol (crp(ph)) and hexachlorocyclohexane (crp(hex)). these crps differ amongst themselves in electrophoretic mobility, and in their carbohydrate content ranging from 20-50%. crps interact with pneumococc ...	2001	12151716
the hatching of common carp (cyprinus carpio l.) embryos in response to exposure to different concentrations of cryoprotectant at low temperatures.	the hatching performance of embryos of the common carp (cyprinus carpio l.) was examined after 1, 7, 14, 21, or 28 days of storage at -8, -6, -4, -2, 0, 2, or 4 degrees c with different concentrations of methanol (0.5-7.0 m in 0.5 m steps) or varying concentrations of methanol in 0.1 m sucrose or trehalose. preserved embryos failed to hatch after storage at -8 and -6 degrees c, regardless of the duration of storage or the concentrations tested. likewise, there was no hatching out above 5.0 m con ...	2002	12151266
duckweed (lemna polyrhiza) leaf meal as a source of feedstuff in formulated diets for rohu (labeo rohita ham.) fingerlings after fermentation with a fish intestinal bacterium.	eight isonitrogenous (35% crude protein approximately) and isocaloric (4.2 kcal g(-1) approximately) diets were formulated including raw and fermented duckweed (lemna polyrhiza) leaf meal at 10%, 20%, 30% and 40% levels. a particular bacterial strain (bacillus sp.) isolated from carp (cyprinus carpio) intestine and having extracellular amylolytic, cellulolytic, proteolytic and lipolytic activities was used for leaf meal fermentation for 15 days at 37 degrees c. the fibre content of leaf meal red ...	2002	12146637
recombinant fish parvalbumins: candidates for diagnosis and treatment of fish allergy.	fish and fish products represent one of the most important causes of ige-mediated food hypersensitivity. in sensitized individuals contact with and consumption of fish can lead to severe health problems, ranging from urticaria and dermatitis to angiedema, diarrhoea, asthma and, at worst, systemic anaphylactic reactions and death. parvalbumin, a small calcium-binding protein present in the muscles of vertebrates, was identified as the major fish allergen. we describe the isolation and characteriz ...	2002	12144564
inhibition of erythrocyte delta-aminolevulinic acid dehydratase (alad) activity in fish from waters affected by lead smelters.	we assessed the effects on fish of lead (pb) released to streams by smelters located in trail, bc (canada), e. helena, mt, herculaneum, mo, and glover, mo. fish were collected by electrofishing from sites located downstream of smelters and from reference sites. blood from each fish was analyzed for delta-aminolevulinic acid dehydratase (alad) activity and hemoglobin (hb), and samples of blood, liver, or carcass were analyzed for pb, zinc (zn), or both. fish collected downstream of all four smelt ...	2002	12139078
sex steroids and their involvement in the cortisol-induced inhibition of pubertal development in male common carp, cyprinus carpio l.	the onset and regulation of puberty is determined by functional development of the brain-pituitary-gonad (bpg) axis. sex steroids produced in the gonads play an important role in the onset of puberty. stress interferes with reproduction and the functioning of the bpg axis, and cortisol has frequently been indicated as a major factor mediating the suppressive effect of stress on reproduction. prolonged elevated cortisol levels, implicated in stress adaptation, inhibited pubertal development in ma ...	2002	12135883
modulation of gene expression in transgenic mouse hearts overexpressing calsequestrin.	calsequestrin (csq) is the major ca2+ binding protein of the cardiac sarcoplasmic reticulum (sr). transgenic mice overexpressing csq at the age of 7 weeks exhibit concentric cardiac hypertrophy, and by 13 weeks the condition progresses to dilated cardiomyopathy. the present study used a differential display analysis to identify genes whose expressions are modulated in the csq-overexpressing mouse hearts to provide information on the mechanism of transition from concentric cardiac hypertrophy to ...	2002	12127059
screen for stage-specific expression genes between tail bud stage and heartbeat beginning stage in embryogenesis of gynogenetic silver crucian carp.	a systemic study was initiated to identify stage-specific expression genes in fish embryogenesis by using suppression subtractive hybridization (ssh) technique. in this study, we presented a preliminary result on screen for stage-specific expression genes between tail bud stage (tbs) and heartbeat beginning stage (hbs) in gynogenetic silver crucian carp (carassius auratus gibelio). two ssh plasmid libraries specific for tbs embryos and hbs embryos were constructed, and stage-specific expression ...	2002	12118939
dopamine and nitric oxide control both flickering and steady-light-induced cone contraction and horizontal cell spinule formation in the teleost (carp) retina: serial interaction of dopamine and nitric oxide.	adaptation to ambient light, which is an important characteristic of the vertebrate visual system, involves cellular and subcellular (synaptic) plasticity of the retina. the present study investigated dopamine (da) and nitric oxide (no) as possible neurochemical modulators controlling cone photomechanical movements (pmms) and horizontal cell (hc) spinules in relation to steady and flickering light adaptation in the carp retina. haloperidol (hal; a nonspecific da receptor blocker) or cptio (a no ...	2002	12115683
fibroin-like substance is a major component of the outer layer of fertilization envelope via which carp egg adheres to the substratum.	seven cdna encoding silkworm fibroin homologues were cloned from a carp ovarian cdna library. the encoded proteins are denoted as carp ovarian fibroin-like substances (fls). fls contain a repetitive domain consisting of tandem repeats of dipeptide of gly-x, where x may be any amino acid. each fls has its own unique repeating sequence, such as gqgagqgs, gqgmgqgm, grgqgeghgs, and gfgfgqgs, indicating a family of fls genes exists in carp. fls is exclusively expressed in oocytes and is stored in cor ...	2002	12112605
thermal plasticity of skeletal muscle phenotype in ectothermic vertebrates and its significance for locomotory behaviour.	seasonal cooling can modify the thermal preferenda of ectothermic vertebrates and elicit a variety of physiological responses ranging from winter dormancy to an acclimation response that partially compensates for the effects of low temperature on activity. partial compensation of activity levels is particularly common in aquatic species for which seasonal temperature changes provide a stable cue for initiating the response. thermal plasticity of locomotory performance has evolved independently o ...	2002	12110664
temperature plasticity of contractile proteins in fish muscle.	three myosin heavy chain isoforms with different actin-activated mg(2+)-atpase activities were found in the fast skeletal muscle from carp (cyprinus carpio) acclimated to 10 and 30 degrees c. the composition of three types of myosin heavy chain was dependent on acclimation temperature, demonstrating the presence of temperature-specific myosin isoforms in carp. subsequently, the temperature-dependence of the sliding velocity of fluorescent f-actin in myosins isolated from 10 degrees c- and 30 deg ...	2002	12110657
association of a new c-cbl related protein with the very first stages of apoptosis induction.	this study investigates the involvement of the c-cbl proto-oncogene during the first stages of the apoptotic process. we have already shown that a c-cbl aptotosis-related protein of 90 kda (carp 90) is detected very rapidly in the cytoplasm as well as in the nucleus of murine thymocytes after hydrocortisone (hc) treatment. we report here that this protein appeared as well after in vivo treatment of mice by gamma-irradiation or injection of anti-cd3 monoclonal antibody, two potent thymic apoptosi ...	2002	12102152
a proposed serotyping system for flavobacterium psychrophilum.	to develop a common serological system for rapid and routine identification of the fish pathogen flavobacterium psychrophilum.	2002	12100595
gene structure of the carp fish ribosomal protein l41: seasonally regulated expression.	the seasonal acclimatization of the carp fish demands physiological compensatory responses. the process involves profound nucleolar adjustments and remarkable changes in rrna synthesis, which affect ribosomal biogenesis. we have documented that protein kinase ck2, whose activity is related to ribosomal protein l41 and the regulation of rrna synthesis, was expressed in notably higher amounts in summer-acclimatized carp compared to the cold-season adapted fish. thus, we approached the study of the ...	2002	12099677
carp oil or oleic acid, but not linoleic acid or linolenic acid, inhibits tumor growth and metastasis in lewis lung carcinoma-bearing mice.	i examined the effects of carp oil, oleic acid, linoleic acid and linolenic acid on tumor growth and metastasis to the liver in mice implanted intrasplenically with highly metastatic lewis lung carcinoma (llc) tumors. carp oil (0.1 or 0.2 ml per mouse) significantly reduced tumor growth and metastasis to the liver. carp oil at 100 or 1000 mg/l inhibited the dna synthesis in llc cells, the capillary-like tube formation of human dermal microvascular endothelial cells (hmvec) at 1000 mg/l and the a ...	2002	12097695