Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| anaerobic pyruvate metabolism of tritrichomonas foetus and trichomonas vaginalis hydrogenosomes. | hydrogenosomes isolated from tritrichomonas foetus and trichomonas vaginalis fermented pyruvate to acetate, malate, h2, and co2 in an anaerobic process dependent on adp, pi, mg2+, and succinate. the extent to which pyruvate was carboxylated to malate by malate dehydrogenase (decarboxylating) rather than decarboxylated to acetate by pyruvate/ferredoxin oxidoreductase was dependent on pco2. the processes observed showed carbon and redox balances. the presence of an nadh/ferredoxin oxidoreductase a ... | 1986 | 3090435 |
| polyamine biosynthesis in trichomonads. | trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum grown in modified diamond's medium all had high concentrations of putrescine and lower concentrations of spermidine and spermine. ornithine decarboxylase (odc; ec 4.1.1.17) was detectable in all three species although at significantly different levels. trichomonas vaginalis had the highest activity (typically around 1.85 nmol min-1 (mg protein)-1), trichomitus batrachorum the lowest (0.11 nmol min-1 (mg protein)-1). the tri ... | 1986 | 3090433 |
| microflora in pregnancy. | 1986 | 3090295 | |
| microbiology of vaginal discharge in general practice. | three groups of women were examined by culture for gardnerella vaginalis and candida. group i consisted of 427 women, who complained spontaneously of vaginal discharge, group ii of 311 women who did not complain of vaginal discharge until questioned prior to gynaecological examination, and group iii of 100 women who denied vaginal discharge. groups i and ii also had cultures made for trichomonas vaginalis and neisseria gonorrhoica. in group i with spontaneous complaints the one-year prevalence r ... | 1986 | 3088694 |
| sexually transmissible infectious agents in sexually active and virginal asymptomatic adolescent girls. | sixty-eight sexually active and 52 virginal adolescent girls were evaluated for six sexually transmissible infectious agents: gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis, trichomonas vaginalis, and neisseria gonorrhoeae. there were significant differences between sexually active and virginal girls with respect to the prevalence of isolation of u urealyticum (75% v 33%, p less than .005), m hominis (27% v 10%, p less than .05), and c trachomatis (19% v ... | 1986 | 3083395 |
| prevalence of nonspecific vaginitis and other cervicovaginal infections during the third trimester of pregnancy. | nonspecific vaginitis (nsv) is a clinical syndrome characterized by a decreased concentration of normal acidophilic döderlein flora in the vagina and increased concentration of nonacidophilic microorganisms, such as gardnerella vaginalis, anaerobic bacteria, and mycoplasmas. since several nsv-associated organisms are associated with complications of pregnancy, we studied the prevalence of nsv among 104 consecutive women during the third trimester of pregnancy. overall, 14 women (14%) had nsv by ... | 1986 | 3082020 |
| [sexually transmitted infections in patients with condylomata acuminata]. | 42 patients (26 males, 16 females) with genital and perianal warts were submitted to serological testing for hbv markers, anti hsv antibodies and syphilis. specimens were also collected for microscopic and cultural examination for neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans and other pathogenic bacteria from urethra in men and from urethra, vagina and cervix in women. women had also cytologic examination from cervix with papanicolau method. we have found ... | 1986 | 3081835 |
| antiparasitic activity of nine pyrazole derivatives against trichomonas vaginalis, entamoeba invadens and plasmodium berghei. | nine nitropyrazole derivatives were prepared and tested against trichomonas vaginalis in vitro and in vivo, entamoeba invadens in vitro and plasmodium berghei in vivo. three of the compounds, 4-4-nitropyrazole, 1-methyl-4-nitropyrazole and 4,4'-dinitro-1,1'-methylenedipyrazole, have an activity similar to that of metronidazole (used as the reference compound) against t. vaginalis and e. invadens after 48 hours of incubation. all the compounds tested were inactive against p. berghei. | 1988 | 3074738 |
| localization of acetylated alpha-tubulin in tritrichomonas foetus and trichomonas vaginalis. | we used monoclonal antibodies specific for acetylated and nonacetylated alpha-tubulin to detect and to localize microtubules containing acetylated alpha-tubulin (stable microtubules) in the pathogenic protozoa tritrichomonas foetus and trichomonas vaginalis. sds-page analysis showed that tubulin is a major protein of both parasites, being enriched in cytoskeletal preparations of whole cells extracted with triton x-100. the monoclonal antibodies, which recognize all isoforms of alpha-tubulin (b-5 ... | 1988 | 3066505 |
| [comparative study of the susceptibility of candida albicans and trichomonas vaginalis, on some products used as larvicides, trichomonacides and/or bactericides]. | 1988 | 3066257 | |
| alternating phenotypic expression of two classes of trichomonas vaginalis surface markers. | the antigenic heterogeneity of trichomonas vaginalis is due in part to the membrane disposition of immunogens (repertoire a) among some but not all isolates and among subpopulations of trichomonads of certain isolates. heterogeneous t. vaginalis isolates undergo phenotypic variation for the a repertoire of immunogens. the presence of immunogens on (a+ phenotype) or the absence of them from (a- phenotype) trichomonal surfaces clearly influences the virulence traits of the pathogenic human trichom ... | 1988 | 3055209 |
| treatment failure in trichomonas vaginalis vaginitis. | 1988 | 3049301 | |
| analysis by immunofluorescence of alpha 1-antitrypsin levels on the surfaces of trichomonas vaginalis strains differing in virulence. | 1988 | 3045793 | |
| new rapid latex agglutination test for diagnosing trichomonas vaginalis infection. | a newly developed latex agglutination test for trichomonas vaginalis infection was compared for sensitivity, specificity, efficiency, and positive and negative predictive values with microscopy, culture, and an enzyme linked immunosorbent assay (elisa) in the diagnosis of 395 women attending a genitourinary medicine clinic. t vaginalis infection was diagnosed in 42 (11%) women. the sensitivities of both the latex agglutination test and the elisa were 95% compared with 74% for microscopy and 76% ... | 1988 | 3045161 |
| [pathogen spectrum of non-gonorrhea urethritis]. | by cultural investigations in 333 males with non-gonorrhoical urethritis (ngu) and 158 control probands it was demonstrated that chlamydia trachomatis is the main germ of this disease also in the gdr with a frequency of 40% of the cases. a chlamydia-negative ngu was caused in 15% by ureaplasma urealyticum in a number of greater than or equal to 10(4) germs/ml urine and in 5% by trichomonas vaginalis, respectively. candida albicans occurred significant more frequently in the patient group (21%) t ... | 1988 | 3043969 |
| nitroimidazole and oxygen derived radicals detected by electron spin resonance in hydrogenosomal and cytosolic fractions from trichomonas vaginalis. | hydrogenosome-enriched fractions from trichomonas vaginalis reduce a number of nitroimidazole derivatives to their respective electron spin resonance-detectable nitro-anion radicals. in the presence of of oxygen and 5,5-dimethyl-1-pyrroline-n-oxide (dmpo) a superoxide spin trapped adduct of dmpo was formed; the rate-determining step was the prior formation of the nitro-anion radical. oxygen-derived radicals were detected with cytosolic fractions from a metronidazole-resistant isolate (cdc-85) wh ... | 1987 | 3041214 |
| phosphomonoesterases of leishmania mexicana mexicana and other flagellates. | amastigotes and log-phase promastigotes of leishmania mexicana mexicana contained distinct acid phosphatase, 3'-nucleotidase and 5'-nucleotidase activities, distinguishable by their response to ph and inhibitors. both tartrate-sensitive and tartrate-resistant acid phosphatase were present in the two forms, amastigotes possessed less tartrate-resistant acid phosphatase than promastigotes. a tartrate-sensitive acid phosphatase was secreted into the medium in large amounts during the growth in vitr ... | 1987 | 3037369 |
| reduction of niridazole by metronidazole resistant and susceptible strains of trichomonas vaginalis. | the inhibitory effect of niridazole on hydrogen production by metronidazole-resistant (cdc-85) and susceptible (c1-nih) trichomonas vaginalis strains was investigated. the results show that niridazole is more effective than metronidazole in inhibiting hydrogen production by the resistant isolate. in cdc-85 aerobic inhibition requires a 4-fold increase in metronidazole concentration compared with that required anaerobically, but the corresponding factor for niridazole is only 1.5-fold. reduction ... | 1987 | 3029651 |
| purine salvage enzymes in trichomonas vaginalis. | 1986 | 3020926 | |
| glycerol, a metabolic end product of trichomonas vaginalis and tritrichomonas foetus. | glycerol was demonstrated as an end product of anaerobic glucose metabolism in trichomonas vaginalis and tritrichomonas foetus, produced in addition to acetate, h2, co2, and lactate or succinate. in t. vaginalis strain c-1, glycerol amounted to 16% of the fermentation products and was formed at an average rate of 38 nmol min-1 (mg protein)-1. corresponding figures for t. foetus strain kv1 were 7% and 4.8 nmol min-1 (mg protein)-1. the amounts of glycerol detected compensated almost exactly for t ... | 1986 | 3016535 |
| [antiviral agents. 28. aliphatic substituted chlordihexylamino-1,3,5-triazine]. | the nucleophilic substitution of one chlorine atom in 2,4-dichloro-6-(dihexylamino)-1,3,5-triazine (1) by primary (2a-b) or secondary (2c) amines leads to the aliphatically substituted chloro-dihexylamino-1,3,5-triazines (3a-c) structures of type 3 may be characterized by the spectroscopic data. in the mass spectra, the complete degradation of the alkyl groupings is striking and manifested in a series of consecutive peaks with differences of 14 between each other. antiviral activity is exhibited ... | 1986 | 3013214 |
| prevalence of genital pathogens among female prostitutes in new york city and in rotterdam. | the authors studied the prevalence of genital microorganisms among 300 female prostitutes in brothels in new york city and 60 female prostitutes attending a sexually transmitted diseases clinic in rotterdam, the netherlands. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum in the two cities were 9.3% and 8.3%, 25.3% and 16.6%, 57.3% and 74.9%, and 73% and 79%, respectively. trichomonas vaginalis was detected in 3.6% of new york pr ... | 1986 | 3012805 |
| screening to reduce transmission of sexually transmitted diseases in semen used for artificial insemination. | the practice of artificial insemination by donor semen is increasing in the united states. many sexually transmitted organisms are found in semen, but screening procedures for the detection of these agents in donor semen have not been standardized. sexually transmitted organisms have been transmitted during artificial insemination by donor, and such transmission can cause local, disseminated, or fatal disease in the recipient woman and may harm the fetus or newborn. therefore, screening of both ... | 1986 | 3010110 |
| [vulvovaginitis. etiopathogenesis and microbiological diagnosis]. | 1986 | 3008032 | |
| respiration of trichomonas vaginalis. components detected by electron paramagnetic resonance spectroscopy. | 1986 | 3007142 | |
| energy-dispersive x-ray microanalysis of membrane-associated inclusions in hydrogenosomes isolated from trichomonas vaginalis. | energy-dispersive x-ray microprobe analysis of electron-dense inclusions in hydrogenosomes isolated from the aerotolerant anaerobic protozoal human parasite trichomonas vaginalis, bushby strain, indicated the presence of high levels of mg, p and ca. this suggested that divalent cation (e.g. ca2+ or mg2+) accumulation by hydrogenosomes may be important in the regulation of intracellular ion concentrations. | 1985 | 3005473 |
| inhibition of hydrogen production in drug-resistant and susceptible trichomonas vaginalis strains by a range of nitroimidazole derivatives. | 1986 | 3000386 | |
| the generation of metronidazole radicals in hydrogenosomes isolated from trichomonas vaginalis. | the nitro radical-anion of the anti-trichomonal drug metronidazole has been detected by electron spin resonance spectrometry under anaerobic conditions in suspensions of intact hydrogenosomes isolated from the parasitic protozoon trichomonas vaginalis. metronidazole reduction was driven by pyruvate, but progressive damage to the radical generating system was observed. quenching of signals due to metronidazole radicals by chromium oxalate suggests that the radicals generated within the organelle ... | 1985 | 2999299 |
| monoclonal antibodies for the diagnosis of sexually transmitted diseases. | monoclonal antibodies are already being used for the diagnosis of human sexually transmitted diseases. these antibodies can be used to detect a wide range of microorganisms, including bacteria, parasites, and viruses. for both culture and direct tests, monoclonal antibodies showed patterns of specificity and reproducibility that exceeded those available with conventionally prepared antisera. the direct tests for these organisms required less than an hour to perform, representing a major advancem ... | 1985 | 2994946 |
| epidemiology of cytomegaloviral infection in a heterogeneous population of pregnant women. | cervical cultures for cytomegalovirus (cmv) and samples of blood for antibody to cmv were obtained from 1,129 pregnant women: 57% of the women had antibody to cmv, and 14% of seropositive women shed virus. logistic regression analysis showed that seropositivity correlated with lower socioeconomic status, birth outside north america, multigravidity, older age, history of abnormal cervical cytology, infection with trichomonas vaginalis, a first pregnancy at less than or equal to 15 years of age, a ... | 1985 | 2993430 |
| isolation and differentiation of herpes simplex virus and trichomonas vaginalis in cell culture. | during the period january 1982 to january 1985, 2,234 specimens were cultured for isolation of herpes simplex virus (hsv). hsv was isolated from 23% of these, trichomonas vaginalis was isolated from 1.6%, and 75.3% were negative. in 0.2% of these, hsv and t. vaginalis were isolated from the same specimen. cytopathic effects produced by hsv were identified by their sensitivity to arabinosylthymine, whereas those produced by t. vaginalis were identified by their lack of sensitivity to arabinosylth ... | 1985 | 2993349 |
| metronidazole radical anion generation in vivo in trichomonas vaginalis: oxygen quenching is enhanced in a drug-resistant strain. | the nitro radical-anion of metronidazole has been detected in vivo in the sexually transmitted human parasite, trichomonas vaginalis, under anaerobic conditions by electron spin resonance spectrometry. exposure of organisms to oxygen decreased the intensity of the radical signal in both metronidazole-sensitive atcc strain 30001 and in the metronidazole-resistant strain 85. the sensitive strain still gave radical signals at partial pressures of oxygen (greater than 6 kpa) sufficient to remove all ... | 1985 | 2985740 |
| a linear double-stranded rna in trichomonas vaginalis. | a "double-stranded" rna was identified in the anaerobic, parasitic protozoan trichomonas vaginalis. electron microscopic evidence indicated linear double-stranded structure 1.5 micron in length, with no apparent hairpins or loops. boiling in 30% dimethyl sulfoxide denatured it into single strands of 1.5 micron and shorter fragments. it consists of 23.4% g, 23.4% c, 23.0% a, and 30.3% u and melts at a transition temperature of 81.7 degrees c in 75 mm nacl and 7.5 mm sodium citrate, ph 7.0, with 7 ... | 1985 | 2982874 |
| the association of sexually transmitted diseases with cervical intraepithelial neoplasia: a case-control study. | thirty-three women with histologically confirmed cervical intraepithelial neoplasia (grades i to iii, with one case of microinvasive carcinoma) and 54 women without evidence of the disease were prospectively studied to determine the relationship of genital infection to cervical neoplasia. demographic and sexual data for patients and control subjects were collected, with standardized clinical and colposcopic evaluation by means of predefined diagnostic categories. cultures from the cervix were ex ... | 1985 | 2982264 |
| evolutionary conservation of laminin-binding proteins. | 1. the virulence of pathogens and metastatic capacity of cancer cells seems to correlate with the ability to adhere to cells and/or to basement membrane components. a key feature of this mechanism is the expression of specific receptors for the basement membrane protein laminin. three different receptors have been already described in cells phylogenetically very distant, such as human white blood cells, trichomonas vaginalis and staphylococcus aureus, all recognizing laminin with the same range ... | 1988 | 2977953 |
| presence of laminin-binding proteins in trichomonads and their role in adhesion. | adhesion is regarded as an important feature in the pathogenesis of various microorganisms. ability to recognize extracellular matrix proteins, such as laminin or fibronectin, has been correlated with invasiveness. we report that laminin enhances the adhesion of the parasitic protozoa trichomonas vaginalis and tritrichomonas foetus to a polystyrene substrate and to the surface of epithelial cells (madin-darby canine kidney cell line) in vitro. the enhancement was higher for t. vaginalis than for ... | 1988 | 2973059 |
| [current therapeutic aspects of trichomonas vaginalis infections in gynecology]. | 1987 | 2972473 | |
| trichomonas infections in men. | trichomonas vaginalis, a common pathogen in the female genital tract, produces a characteristic clinical picture in women. less well recognized are the manifestations of trichomonas infestations of the male genital tract, which include urethritis and chronic prostatitis. multiple-glass urinalysis and selective use of trichomonas cultures may improve recognition of this organism in the family practice setting. | 1989 | 2916396 |
| [synthesis and antiparasitic activity of arylproprenones in the nitro-5-imidazole series]. | the synthesis of new arylpropenones derived from 1-methyl-5-nitro imidazole is described. the investigation of some antiprotozoal properties has shown that compounds with the groups 4-hydroxyphenyl, 4-chlorophenyl or thiophenyl have trichomonacidal activity similar to that of metronidazole. | 1988 | 2904887 |
| sexually transmitted diseases in women with urethral syndrome. | frequency of micturition and dysuria were prominent symptoms in 135 (57%) of 237 women with urethral syndrome. ureaplasma urealyticum, mycoplasma hominis and chlamydia trachomatis were the principal organisms associated with the urethral syndrome (38.41%, 28.14% and 11.11%, respectively). escherichia coli was cultured from four patients and herepes genitalis and neisseria gonorrhoea were isolated from five patients. infections with more than one organism were frequent. thirty-one of 135 patients ... | 1988 | 2903082 |
| trichomonas vaginalis: characterization of its glutamate dehydrogenase. | an nadp-linked glutamate dehydrogenase (ec 1.4.1.4) was found in the soluble fraction of trichomonas vaginalis. its molecular weight was about 230,000 (gel filtration). the enzyme, partially purified by diafiltration and hydroxyapatite column chromatography, was heat stable (1 hr at 57 c). it catalyzed both the amination of alpha-ketoglutarate (mean km 0.6 mm) and the deamination of glutamate (mean km 1.2 mm) the optimum ph of the amination reaction was 6.7, and that of the deamination reaction ... | 1988 | 2901980 |
| unloaded polyisobutylcyanoacrylate nanoparticles: efficiency against bloodstream trypanosomes. | the potential use of polyisobutylcyanoacrylate nanoparticles in antiparasitic chemotherapy is described. the nanoparticles on their own proved to have trypanocidal activity against trypanosoma brucei brucei in-vitro but not against trichomonas vaginalis or entamoeba histolytica. the trypanocidal activity was partly confirmed in-vivo with t. brucei-infected mice. | 1987 | 2888859 |
| action of anticytoskeletal compounds on in vitro cytopathic effect, phagocytosis, and adhesiveness of trichomonas vaginalis. | the cytopathic effects of trichomonas vaginalis treated with inhibitory concentrations of anticytoskeletal compounds (mebendazole, griseofulvin, colchicine, taxol, and cytochalasin b) were studied in mouse clid fibroblast cultures. the evaluation, at different times, of cell numbers and morphological alterations showed that cytopathic effect was considerably reduced when protozoa were pretreated with mebendazole and griseofulvin, whereas colchicine, taxol, and cytochalasin b had less effect. fur ... | 1987 | 2888725 |
| [synthesis and antiparasitic activity of new nitro-5-imidazole derivatives]. | syntheses are given for derivatives of the type: 2-amino-1-[(1-methyl-5-nitro-2-imidazolyl) methyleneimino] imidazole, of new hydrazones of 1-methyl-5-nitroimidazole-2-carboxaldehyde and also for semicarbazones and a hydrazone of 1-methyl-5-nitro-2-(4-formyl-styryl)imidazole. | 1987 | 2888689 |
| [synthesis of new diheterocyclic derivatives of imidazole with antiparasitic activity]. | a description is given of potential cyclic imidazoles such as 3,3-dichloro-2-methylenimino-acrylonitrile derived from nitro or non-nitro heterocycles, as well as the hydrazone, semicarbazone and arylpropenone derivatives of the 4-chloro-2-(5-nitro-2-thienyl) (v), 5-imidazole carboxaldehyde (iv) and the 5-bromothienyl analogue. the antiparasitic activity was tested in vitro against trichomonas vaginalis and entameba histolytica. some of the nitro containing compounds showed trichomonacidal activi ... | 1987 | 2886364 |
| use of monoclonal antibodies to identify, characterize, and purify a 96,000-dalton surface antigen of pathogenic entamoeba histolytica. | we identified and partially characterized a surface antigen of entamoeba histolytica by using seven monoclonal antibodies obtained after injecting mice with a pathogenic strain of amoeba. an intrinsically radiolabeled 96,000-dalton antigen was immunoprecipitated by five of the seven monoclonal antibodies; this antigen was present in three strains of e. histolytica. the antigen was situated on the external surface of e. histolytica, as demonstrated by agglutination and immunofluorescence staining ... | 1987 | 2885384 |
| relationship of vaginal ph and papanicolaou smear results to vaginal flora and pregnancy outcome. | prematurity is a major cause of perinatal morbidity. studies have implicated components of the vaginal flora in the etiology of some cases of preterm birth. current scoring systems do not include factors which directly reflect the vaginal flora. since papanicolaou smears and the vaginal ph may be affected by the vaginal flora and are easy tests to perform, we studied their relationship to vaginal flora and pregnancy outcome. among 231 patients, those with a vaginal ph greater than or equal to 4. ... | 1987 | 2883041 |
| [pathogen-host relations in sexually transmitted diseases]. | the host-parasite relationship between the major causative agents of sexually transmitted diseases, e.g. neisseria gonorrhoeae, treponema pallidum and trichomonas vaginalis, and their target cells in the urogenital tract can generally be studied in suitable cell and organ cultures. experience with n. gonorrhoeae predominantly gained from fallopian-tube organ cultures shows that there are several prerequisites for adherence as the first step of pathogenesis. they range from the type of bacterial ... | 1986 | 2878907 |
| the possible role of trichomonas vaginalis as a "vector" for the spread of other pathogens. | 1986 | 2878896 | |
| modulation of amino acid and 2-oxo acid pools in trichomonas vaginalis by aspartate aminotransferase inhibitors. | the amino acid pool sizes of trichomonas vaginalis are reported. alanine, glutamic acid, proline and leucine account for 72% of the measured amino acids. growth of t. vaginalis was unaffected by gostatin, an irreversible inhibitor of aspartate aminotransferase, when the enzyme activity within the cell had been completely inhibited and a specific elevation of the aspartate pool had occurred. in media lacking aspartate and glutamate, the amino acid substrates of the aspartate aminotransferase reac ... | 1986 | 2877395 |
| aspartate:2-oxoglutarate aminotransferase from trichomonas vaginalis: comparison with pig heart cytoplasmic enzyme. | the aspartate:2-oxoglutarate aminotransferase from the protozoon trichomonas vaginalis exists as a mixture of sub-forms of identical mr and amino acid composition, and of similar catalytic properties. the amino acid composition closely resembles that of aspartate aminotransferase from prokaryotic and vertebrate sources. some molecular and catalytic properties of the t. vaginalis aspartate aminotransferase are compared with those of the cytoplasmic pig heart enzyme. a major difference is in the a ... | 1986 | 2876843 |
| inhibition of trichomonas vaginalis replication by the microtubule stabilizer taxol. | taxol, a plant alkaloid stabilizer of microtubules, inhibits in vitro the replication of the human pathogenic flagellate trichomonas vaginalis in a dose-dependent fashion. micromolar concentrations of the drug induce massive assembly of microtubules, resistant to antimicrotubule agents, and block the mitosis of the protozoa at an early stage preceding the formation of the spindle fibers and the depolymerization of the axostylar microtubules. some hypotheses involving the inactivation of the micr ... | 1986 | 2874220 |
| [in vitro effect of nonoxynol-9 on herpes virus 1 and 2 and on other sexually transmitted micro-organisms]. | 1988 | 2854228 | |
| frequency of cervico-vaginal infections (trichomonas vaginalis; chlamydia trachomatis -chl-; herpes simplex virus -hsv-; human papilloma virus -hpv-) in cervical intraepithelial neoplasia. | the authors have carried out a cyto-hystologic study on 533 cervical intraepithelial neoplasias (cin) devoted to identifying the association frequency between cervical dysplastic lesions and cervico-vaginal infections caused by trichomonas vaginalis, chlamydia trachomatis, herpes simplex virus and human papilloma virus. hpv lesions have revealed the pathology found more frequently in cin lesions (33.2%) compared to 0.19% in the normal population, while the other infections have not shown signifi ... | 1988 | 2839342 |
| itraconazole: increased activity by chlorhexidine. | chlorhexidine increases the activity of itraconazole against candida isolates; itraconazole-chlorhexidine combinations show synergistic activity in culture media. the activity of itraconazole is discussed. | 1988 | 2839324 |
| hydrogenosomal atp:amp phosphotransferase of trichomonas vaginalis. | 1. atp:amp phosphotransferase (adenylate kinase) is present in trichomonas vaginalis, primarily with hydrogenosomal localization. 2. adenylate kinase has been purified from hydrogenosome-enriched fractions by solubilization with triton x-100 and kcl followed by affinity chromatography and gel filtration. 3. the enzyme has a mr = 28,000, a broad ph optimum of ph 7-9, requirement for mg2+ and specificity for adenine and deoxyadenine nucleotides. 4. the activity is competetively inhibited by p1,p5- ... | 1987 | 2827949 |
| characterization and purification of extracellular proteases of trichomonas vaginalis. | extracellular protease activity was detected in serum-free culture filtrates of trichomonas vaginalis. the activity was demonstrated by hydrolysis of hide powder azure and possessed the characteristics of cysteine type proteases: inhibition by n-ethyl maleimide, cu2+, antipain, n-tosyl-l-phenylalanine chloromethyl ketone, n-tosyl-l-lysine chloromethyl ketone, leupeptin, chymostatin, and iodoacetamide, and enhancement by cysteine, edta, and dithiothreitol. the activity was optimal at acid ph and ... | 1989 | 2819601 |
| chlamydia trachomatis cervical infection and oral contraceptive use among adolescent girls. | this study examines the relationship between oral contraceptive use and chlamydia trachomatis cervical infection in women less than 19 years of age. the clinical and epidemiologic data of 73 (19.4% prevalence rate) girls with chlamydial infection were analyzed, with special attention given to contraceptive use. the findings were compared with data from 303 girls who were chlamydia negative to assess the relationship between oral contraceptive use and c. trachomatis infection. use of an oral cont ... | 1989 | 2808080 |
| sexually transmitted pathogens in pregnant women in a rural south african community. | one hundred and ninety three consecutive pregnant women attending peripheral antenatal clinics attached to ngwelezana hospital, empangeni, kwa-zulu, were examined for evidence of sexually transmitted pathogens. the following incidences were found: trichomonas vaginalis 49.2% (95), candida spp 38.3% (74), chlamydia trachomatis 11.4% (22), gardnerella vaginalis 6.2% (12), neisseria gonorrhoeae 5.7% (11), positive syphilis serology results 11.9% (23), hepatitis b surface antigen 4.1% (eight). no wo ... | 1989 | 2807289 |
| failure of mebendazole to cure trichomonal vaginitis resistant to metronidazole: case reports. | after a recent report showing the in vitro susceptibility to mebendazole of a strain of trichomonas vaginalis that was resistant to metronidazole, we present two cases of metronidazole resistant infection, both of which failed to respond to oral mebendazole. | 1989 | 2807288 |
| [the microbial flora of the vagina in trichomoniasis]. | the vaginal flora was investigated in women with trichomoniasis by means of a culture method. trichomonas, yeast-like fungi and various types of bacteria were isolated. the applied microbiological technique allowed isolation not only of aerobic and cicrophilic bacteria but only of anaerobic bacteria. anaerobic bacteria were nonspore-forming and were isolated very frequently in the vagina. | 1989 | 2802080 |
| identification of virus-like particles in eimeria stiedae. | when nucleic acid samples purified from sporozoites of eimeria stiedae were analyzed by agarose gel electrophoresis, an ethidium-stainable band with an apparent electrophoretic mobility of 6.5 kb was consistently observed. the band was readily degradable upon rnase treatment, and its susceptibility towards ribonuclease a on a decreasing ionic strength was suggestive of double-stranded rna (dsrna). electron microscopy revealed spherical, probably icosahedral, virus-like particles (vlp) with a dia ... | 1989 | 2797059 |
| trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells. | the role of cysteine proteinases in adherence of trichomonas vaginalis nyh 286 to hela and human vaginal epithelial cells was evaluated. only pretreatment of trichomonads, but not epithelial cells, with n-alpha-p-tosyl-l-lysine chloromethyl ketone (tlck), an inhibitor of trichomonad cysteine proteinases, greatly diminished the ability of t. vaginalis to recognize and bind to epithelial cells. leupeptin and l-1-tosylamide-2-phenylethyl chloromethyl ketone, other cysteine proteinase inhibitors, al ... | 1989 | 2789190 |
| isolation of a cell-detaching factor of trichomonas vaginalis. | the pathogenetic role of soluble products of trichomonas vaginalis growth in culture is controversial. to evaluate this role, t. vaginalis was grown in broth and cell culture and the cell-free filtrate was applied to fresh cell culture monolayers. when adjusted to ph 6.5, filtrates obtained from 22-h culture growth totally disrupted mccoy, hep-2, human foreskin fibroblast, and chinese hamster ovary cell monolayers within 6 h. these detached cells remained greater than 90% viable. this cell-detac ... | 1989 | 2788661 |
| characterization of a secreted cytoactive factor from trichomonas vaginalis. | trichomonas vaginalis, grown in dulbecco's modified eagle's medium with or without serum, produced a factor (tvf) which altered the morphology of certain mammalian cells in vitro. tvf had a mr of approximately 250 kda by gel filtration, approximately 50 kda by sds-page, and was heat (56 degrees c, 30 min) and ph (greater than 6 or less than 8) labile. co-incubation of tvf with adherent target cells caused a marked rounding and clumping of bhk-21 or cho-k1 cells, but had no effect on rk-13 or weh ... | 1989 | 2788372 |
| trichomonas vaginalis and tritrichomonas foetus secrete neuraminidase into the culture medium. | supernatants taken from axenic cultures of trichomonas vaginalis and tritrichomonas foetus contain a neuraminidase activity, the detection of which is augmented when the trichomonad culture media are supplemented with 30% supernatant of confluent epithelial cultures. the enzyme was active against human erythrocytes, which became highly reactive to peanut agglutinin lectin. the specificity of the enzyme was checked by using a substrate specific to neuraminidase: 2'-(4-methylumbelliferyl)-alpha-d- ... | 1989 | 2788244 |
| evaluation of six media for the growth of trichomonas vaginalis from vaginal secretions. | many media have been formulated for the growth of trichomonas vaginalis, but the relative sensitivities of these media have not been determined. we evaluated the ability of six media, including all five media commercially available in the united states, to grow trichomonas vaginalis from vaginal secretions. in a first experiment, we evaluated the ability of five media to grow t. vaginalis from vaginal secretions of 375 women and determined the optimal days on which to read culture tubes, by inoc ... | 1989 | 2787804 |
| survival of trichomonas vaginalis in human semen. | although exposure of trichomonas vaginalis to human semen is of short duration, any effect that this fluid may have on the urogenital protozoon could affect its transmission, especially if only few trichomonads are present. small numbers of parasites (about 2500/ml semen) incubated in semen from different donors at 37 degrees c, were found to survive or grow for up to 12 hours in all samples and for up to 24 hours in most. survival and growth of t vaginalis in semen most resembled that found in ... | 1989 | 2787774 |
| secretory hydrolases of trichomonas vaginalis. | supernatants of three trichomonas vaginalis strains obtained from culture or after contact and cultivation with wish cells were assayed to characterize the nature of the factor/s responsible for the cytotoxic activity on wish cell monolayers. this factor, mainly secreted from the live, exponentially grown parasite, with a molecular weight higher than 10,000 daltons and heat-unstable, does not appear to be a protease. in its place, we demonstrated that the presence of some hydrolases (particularl ... | 1989 | 2787467 |
| comparison of diamond's medium modified and kupferberg medium for detection of trichomonas vaginalis. | diamond's medium modified, the commercially available version of the klaas modification of diamond's medium, was compared to kupferberg medium and to direct wet-mount examination for detection of trichomonads in symptomatic patients. diamond's medium was found to be superior to both kupferberg medium (p less than 10(-4) and wet mount (p less than 10(-6]. | 1989 | 2787332 |
| [observation of the processes of phagocytosis and digestion of staphylococcus aureus by trichomonas vaginalis using a transmission electron microscope]. | trichomonas vaginalis was incubated with bacteria for 30 min. after centrifugation of staphylococcus aureus, incubation of t. vaginalis was continued for 40 min., 120 min. and 6 hrs. it was found that bacteria have been intensively phagocytated through whole surface of protozoan. phagosome with bacteria at different stages of degradation, up to defecation of undigested residues were observed. it was observed that some bacteria, apparently not susceptible to digestion, were immersed directly in c ... | 1989 | 2786283 |
| relatedness of structures of a major immunogen in trichomonas vaginalis isolates. | solubilization of live trichomonas vaginalis organisms with detergent caused the release of cysteine proteinases in the detergent extract which were inhibitable with n-alpha-p-tosyl-l-lysine chloromethyl ketone. the detergent extracts of all isolates tested possessed similar cysteine proteinase activities. these parasite proteinases rapidly degraded a prominent immunogen whose surface disposition undergoes phenotypic variation in some isolates. the relatedness of the forms of this immunogen amon ... | 1989 | 2785964 |
| scanning electron microscopy of trichomonas vaginalis. | by scanning electron microscopy (sem), t. vaginalis surface appeared pleated with crater-like depressions. all the flagella appeared to start off in a single bundle from a shallow depression with a prominent rim. the side carrying the undulating membrane is extensively ruffled having a mulberry-like appearance to which flocculent material was frequently adherent. t. vaginalis nutrition appeared to proceed through pseudopodia-like extensions and phagocytosis by a constant deep invagination at the ... | 1989 | 2785150 |
| clinical manifestations of vaginal trichomoniasis. | trichomonas vaginalis was detected by culture or wet-mount examination in 118 (15%) of 779 randomly selected women attending a sexually transmitted disease clinic. vaginal trichomoniasis was significantly associated with symptoms of yellow discharge, abnormal vaginal odor, and vulvar itching and with signs of colpitis macularis ("strawberry cervix"), purulent vaginal discharge, and vaginal and vulvar erythema. a logistic regression model was used to adjust for coinfections, as well as for demogr ... | 1989 | 2783346 |
| differences in biologic maturation, sexual behavior, and sexually transmitted disease between adolescents with and without cervical intraepithelial neoplasia. | because several cofactors may influence the development of cervical intraepithelial neoplasia (cin) in young women, we compared differences in behaviors (sexual activity, contraception, and cigarette use), sexually transmitted disease (std) infection rates (chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and herpes simplex virus), and cervical maturation (age of menarche and percentage of cervical ectopy) in groups of sexually active female adolescents with and without cin. ... | 1989 | 2769512 |
| diagnostic procedures in vaginitis. | ninety-seven vaginitis patients underwent a standardized direct method of examination. bacterial vaginosis was found in 38.1%, candida albicans in 24.7% and trichomonas vaginalis in 13.4%. low secretional ph values were never met in bacterial vaginosis and t. vaginalis cases. a fishy odor, present in bacterial vaginosis, was also found in almost half of the t. vaginalis cases. endocervical bacteriological cultures are probably insufficient with respect to the detection of c. albicans and gardner ... | 1989 | 2759324 |
| infection with trichomonas vaginalis in a black population. | trichomonas vaginalis infection (tvi) was found by examination of pap smears in 25% of 3,005 unselected urban black women being screened for cancer of the cervix and vagina. the incidence was 22% in the group under 29 years of age; 69% in those between 30 and 59; and 9% in those over 60. women who had had a hysterectomy had 16% lower incidence of tvi than did the controls. class 1 reports (atypia, metaplasia, hyperplasia) were obtained in 10% of the entire group. cytologic changes were present i ... | 1989 | 2746693 |
| association of chlamydia trachomatis and mycoplasma hominis with intrauterine growth retardation and preterm delivery. the john hopkins study of cervicitis and adverse pregnancy outcome. | the authors conducted a prospective study of risk factors for intrauterine growth retardation (birth weight less than a standard (race- and sex-adjusted) 10th percentile for gestational age) and preterm birth (birth prior to 37 weeks gestation) in a high-risk, inner-city, obstetric population, with special interest in pathogens colonizing the maternal genital tract. a total of 801 women within 22 to 30 weeks of gestation were enrolled and interviewed. subjects were cultured for gardnerella vagin ... | 1989 | 2729260 |
| evaluation of proposed cytomorphologic criteria for the diagnosis of chlamydia trachomatis in papanicolaou smears. | to evaluate the proposed cytomorphologic criteria for the cervical cytologic diagnosis of chlamydia trachomatis infection, a study was made of 171 endocervical smears. all cytomorphologic elements that could be ascribed to chlamydia trachomatis infection were correlated with the diagnostic confirmation of this microorganism by monoclonal antibody (mab) staining. the presence of chlamydia trachomatis was detected in 21 samples (12.28%) by mab staining. comparing the cytomorphologic results with t ... | 1989 | 2728785 |
| new derivatives of 5-nitroimidazole: synthesis and antiparasitic activity. | the synthesis and the antiparasitic evaluation of twelve new 5-nitroimidaole derivatives has been carried out. the most effective compounds were the less hydrophilic pyridinium and imidazolium salts (iv), (v) and (x), and above all the tetrahydropyridine derivatives (xii) and (xiii). | 1989 | 2701965 |
| bacterial vaginosis. | bacterial vaginosis is a common, non-inflammatory infection of the vagina. it is characterised by the presence of a thin, homogenous, greyish-white discharge. the differential diagnosis includes infection with trichomonas vaginalis and candida albicans. a diagnosis of bacterial vaginosis may be made by the detection of three of the following: characteristic discharge, ph of 5 or greater, clue cells and a positive koh amine test. culture of vaginal discharge is not necessary to effect a diagnosis ... | 1989 | 2694618 |
| [use of native microscopic examination of the vaginal flora in the diagnosis of trichomoniasis]. | microscopic diagnosis of vaginal discharge established so far by means of microbial vaginal pictures (mvp) has proved to be insufficient. phase contrast microscopy has an irreplaceable value in diagnosing vaginal infections transmitted by sexual intercourse. it yields quick and exact diagnosis of the disease, thus providing the possibility to administer treatment addressly and immediately. in equivocal findings and relapses vaginal cultivation is indispensable. in a series of 600 examined women ... | 1989 | 2691022 |
| [retrospective study of mycotic and other colpitis pathogens]. | we had 170 female patients with presenting symptoms of vaginal itching, dysuria, dyspareunia, burning and vaginal discharge. we performed 200 times vaginal microbial exploration on the said 170 patients, with the repetition of the procedure on 30 out of these 200 patients for the second time. we had a positive result for vulvovaginal candidosis on 60 occasions. these positive results for yeast infection can be related to the incidence of other microbial infections such as mycoplasma-, ureaplasma ... | 1989 | 2688322 |
| trichomonas vaginalis. | trichomonas vaginalis is one of the most common sexually transmitted diseases. the epidemiology, diagnosis, treatment, and concerns about transmission are discussed. | 1989 | 2687745 |
| vaginitis/vaginosis. | vaginitis/vaginosis is an extremely common medical problem. most cases are caused by yeast (predominantly candida albicans), the protozoan trichomonas vaginalis, or a specific mixture of bacteria (bacterial vaginosis). the prevalence of each of these varies with the patient population. the clinical signs used in differentiating among these and the associated bacterial flora are shown in table 3. because vaginitis/vaginosis cannot be adequately diagnosed solely on the basis of symptoms or physica ... | 1989 | 2676321 |
| trichomonas vaginalis. | although not life-threatening, trichomoniasis is a major health problem, especially in women. it is transmitted sexually and has a very high incidence worldwide. we argue that trichomonas vaginalis is not a commensal. attempts should be made to identify this pathogen in all suspicious cases. although culturing remains the most sensitive method to detect t vaginalis, newer methodologies such as monoclonal antibody fluorescent and direct staining make it possible to diagnose an infection with high ... | 1989 | 2668827 |
| carbohydrate, energy and hydrogenosomal metabolism of tritrichomonas foetus and trichomonas vaginalis. | 1989 | 2657037 | |
| differentiation of gardnerella vaginalis, candida albicans, and trichomonas vaginalis infections of the vagina. | this study evaluated the positive predictive values of factors associated with gardnerella vaginalis, candida albicans, and trichomonas vaginalis for diagnosing vaginitis in a community-based population. one hundred ninety-six women with and without vaginal complaints were evaluated for historical factors, physical examination findings, and office laboratory results that were potentially associated with each of the three vaginal organisms. extensive microbiological tests were performed to detect ... | 1989 | 2656903 |
| the emergency department diagnosis of trichomonas vaginitis. | trichomonas vaginalis is a common cause of vaginitis in emergency department patient populations. while the diagnosis of this condition is usually on the basis of wet-mount microscope slide preparations, other diagnostic technology exists. we studied the accuracy of the traditional wet-mount technique as compared with detection using direct immunofluorescence and culture techniques in ed patients with the presenting complaint of vaginal discharge. of 157 patients evaluated, the wet-mount techniq ... | 1989 | 2655509 |
| [epidemiologic aspects of trichomoniasis]. | 1989 | 2655345 | |
| epidemiology of sexually transmitted diseases in rape victims. | rape is a violent crime that is increasing rapidly in incidence. victims can include male and female adults and children. these victims may be at risk for the acquisition of many sexually transmitted conditions. the risk will vary with the sex and socioeconomic status of the assailant as well as with the nature of the sexual contact. infections with neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, herpes simplex virus, human papillomavirus, and treponema pallidum have all bee ... | 1989 | 2649960 |
| evaluation and management of vaginitis. an update for primary care practitioners. | there are three major causes of vaginitis symptoms that primary care practitioners should be able to diagnose and treat expertly. recent investigations have shown that bacterial vaginosis, the most common cause of vaginitis symptoms in patients not seen in sexually transmitted disease clinics, has a multifactorial etiology: gardnerella vaginalis is universally present but is not, per se, the etiologic agent. diagnosis and treatment are based, therefore, on evidence of a disturbed bacterial ecolo ... | 1989 | 2645843 |
| glycogen medium, antitrichomonal drug activity in vaginal liquids. | the amount of glycogen in vaginal liquids decreases with trichomonas vaginalis and this is connected with t. vaginalis activity in specimens. a glycogen-hydrolysed casein medium ("glycogen medium") added to vaginal liquid is a valid maintenance medium for t. vaginalis and therefore enables a direct test for antitrichomonas drugs to be performed. | 1989 | 2637795 |
| study of candida and trichomonas vaginalis in leucorrhoea. | of 150 clinically suspected cases of leucorrhoea, 44 cases (29.33%) showed growth of candida and 30 cases (20%) of trichomonas, by wet preparation. among the candida species the most frequent isolate was c albicans (56.8%), followed by c tropicalis (20.4%), c krusei (11.4%), c stellatoidea (9.1%) and c parapsilosis (2.3%). the higher incidence was found among the pregnant women than among non-pregnant women. among pregnant women multipara in third trimester showed higher incidence of candida and ... | 1989 | 2621360 |
| prevalence of common infective agents of vaginitis. | a total of 100 women with vaginitis and 50 age matched healthy women were studied for common infective agents of vaginitis. the vaginal secretions collected from these women were examined for trichomonas vaginalis, g. vaginalis, and candida species. the prevalence of infective agents in the patients was t. vaginalis 11 per cent. candida species 30 per cent and gardnerella vaginalis 31 per cent. in contrast, in the control group the prevalence was candida species 14 per cent and g. vaginalis 22 p ... | 1989 | 2614050 |
| trichomonas vaginalis infection in a lesbian. | 1989 | 2613220 | |
| [synthesis of 2-aryl benzothiazoles and research on its antiparasitic activity]. | several 2-aryl benzothiazoles (with phenyl, naphthyl, thienyl and some oxygenated heterocycles) were synthesized. the anti-parasitic properties of these compounds were evaluated, in vitro and in vivo, against one nematode nippostrongylus brasiliensis and, in vitro against two protozoaires entamoeba histolytica and trichomonas vaginalis. only two compounds exhibited, in vitro a low nematicidal activity. | 1989 | 2610470 |
| [the diagnostic value of a culture study in detecting patients with trichomoniasis]. | the advantages of culture method for the diagnosis of trichomoniasis over microscopy of stained and native preparations are demonstrated. new liquid nutrient media for trichomonas cultivation reveal vaginal trichomonas in 99.48% of cases in medium, in 100% of cases in medium, and in 97.78% of cases. these media are universal and may be used at bacteriologic laboratories of treatment-and-prophylaxis institutions engaged in culture diagnosis of trichomoniasis. | 1989 | 2609750 |
| comparative in vitro sensitivity to metronidazole of trichomonas vaginalis with isolates and clones. | isolates of trichomonas vaginalis from a group of thai women in khon kaen, thailand were studies for their in vitro sensitivity to metronidazole. forty-five isolates of t. vaginalis and further 226 laboratory clones of these isolates were examined for drug sensitivity under anaerobic conditions using the cplm (cysteine-peptone-liver infusion maltose) medium. the in vitro sensitivity of t. vaginalis to metronidazole, recorded as mlc values revealed highly variable sensitivity profiles greater tha ... | 1989 | 2609217 |
| endocervical chlamydial infection in women attending a sexually transmitted disease clinic in singapore. | two hundred women attending the sexually transmitted disease (std) clinic at middle road hospital were investigated. chlamydia trachomatis was isolated from 32% of women who were contacts of men with nongonococcal urethritis, 15% of contacts of gonococcal urethritis, 27% of contacts of unspecified std, and 13% of women without any history of std in their sex partners. overall, chlamydia trachomatis was isolated from 17% of 200 women, neisseria gonorrhoeae from 13% of 199 women, candida albicans ... | 1989 | 2609175 |