Publications

effects of bcg and corynebacterium parvum on immune reactivity in melanoma patients.	sixteen patients with disseminated melanoma were immunised with either bcg (8 cases) or c. parvum (8 cases) on three occasions at 21 day intervals. blood for assay was taken immediately before the first immunisation and weekly for eight weeks thereafter. total white count tended to increase but little change was seen in lymphocyte and monocyte counts. serum igg increased after bcg but not with c. parvum, serum iga and igm did not alter. the 'e' rosette % did show some increase mainly after c. pa ...	1977	608536
effects of corynebacterium parvum vaccine on drug metabolism in the mouse.	mice of the cba strain were given a single intraperitoneal injection of c. parvum vaccine at 50 mg/m2. there followed a progressive increase in liver weight and in the duration of hexobarbitone sleeping time, accompanied by a marked decrease in liver microsomal protein and in cytochrome p-450/mg microsomal protein. that is to say, content of cytochrome p-450 was reduced out of proportion to the apparent loss of endoplasmic reticulum. these changes were paralleled by decreases in microsomal o-dem ...	1977	608533
histological changes of intravascular coagulation in mice resulting from intravenous injection of c. parvum.	a histological study was made of liver and other tissues of mice at intervals after injection of c. parvum. as early as 20 min after injection occasional thrombi surrounded by polymorphs were seen in the alveolar capillaries. a general thrombosis rapidly developed in the hepatic vessels with consequent hepatic necrosis. thrombi also developed in splenic vessels. this intravascular coagulation was prolonged, lasting at least 7 days. there was evidence that fresh thrombi were being formed througho ...	1977	608532
comparison of bcg and c. parvum preparations for adjuvant contact therapy of a rat sarcoma.		1977	608529
the local effect of intravesical corynebacterium parvum in the dog. a preliminary study.	high doses of c. parvum introduced in the bladder of the dog provoke changes in the submucosa. the significance and importance of these modifications should be investigated.	1977	608526
effects of bcg and c. parvum treatment on human melanoma xenografts.	cba mice rendered immunodeficient by thymectomy, potentially lethal gamma-irradiation and reconstitution with bone marrow cells were used to grow a wide variety of human tumours as subcutaneous implants. samples of human melanoma obtained at surgery were successfully passaged by transplantation and produced rapidly growing tumours, some of which metastasized to lung, lymph nodes and the para-aortic node; this system was used as a model for the study of immunotherapy of melanoma. preliminary resu ...	1977	608525
the effect of pretreatment with corynebacterium parvum on the growth of a chemical induced transplanted murine tumor.	unspecific immunostimulation with or without specific tumor immunotherapy may well contribute to the control of minimal residual cancer. corynebacterium parvum (c.p.) has been shown to raise the level of immunocompetence in tumor bearing animals. experiments are reported, which aim at an evaluation of optimal conditions for an immunostimulation with c.p. prior to the transplantation of balb/c meth a ascitis. using different dosages, routes of injection and schedules of vaccination it was found, ...	1977	608519
experimental evaluation of nonspecific immunostimulation and chemotherapy in the intracranial tumor-bearing fisher rat.	one hundred sixty rats bearing intracranial tumors were divided into groups of 10 to evaluate c-i interval +, effect of chemotherapy alone (bcnu), chemotherapy and c. parvum, and c. parvum alone in the rat tumor model. all therapy was administered intraperitoneally: bcnu -5.2 mg/kg (40% of ld10) on the 9th and 16th post tumor implant day, and c. parvum-1.4 mg regardless of the body weight and the 6th or 23rd day and 6th and 23rd post tumor implant day. an additional group received bcnu on the 9t ...	1977	608518
increased sensitivity of c. parvum treated mice to ionizing whole body irradiation.	we have investigated whether c. parvum (cp)-induced stimulation of hematopoiesis affects the survival of mice exposed to the whole body irradiation (wbi). c3hf/bu mice treated with cp exhibit an increased hematopoietic colony forming activity in their spleens and blood, but not in their bone marrow, as determined by the exogenous spleen colony assay. also, cp-treated c3hf/bu as well as cba mice show an increase in their endogenous colonies. this increased hematopoietic activity caused by cp trea ...	1977	608517
chemo-immunotherapy of mouse tumours using cyclophosphamide and c. parvum.	treatment of a chemically induced mouse solid fibrosarcoma using either non-specific (c. parvum 350 mu i.v.), or specific active (s.c. c. parvum mixed with 5.10(5) irradiated tumour cells) immunotherapy, 4 days after a single dose of cyclophosphamide (200 mg/kg) was synergistically more effective than either c. parvum or drug treatment alone. a contributory factor may be that cyclophosphamide pretreatment has been shown to potentiate the specific antitumour immunity that arises from c. parvum in ...	1977	608516
effects of c. parvum on radiation response of murine tumors.	the effects of intravenous c. parvum (cp) on the efficacy of fractionated gamma-irradiation in treating a fairly immunogenic fibrosarcoma (fsa) and a weakly immunogenic mammary carcinoma (mdah-mca-4) were studied in c3hf/bu mice. tumors were 8 mm in diameter at the start of irradiation. the fsa was exposed to 500 and the mdah-mca-4 to 750 rads daily for 3.6 or 10 days. the administration of 0.25 mg cp varied from 4 days before to 14 days after the start of irradiation. cp greatly augmented radio ...	1977	608515
in vivo transfer of antitumor activity by peritoneal exudate cells from mice treated with c. parvum.	we have investigated whether peritoneal exudate cells (pec) from c. parvum (cp) treated (c3hf/bu mice could transfer in vivo the resistance against a syngeneic fibrosarcoma (fsa). inhibition of tumour development and prolongation of survival of recipients were observed when cp-activated pec were admixed with fsa cells before their intraperitoneal (ip) or subcutaneous (sc) injections into normal mice. the antitumour activity increased with the increase of the ratio of effector to target cells. he ...	1977	608514
a comparison of the antitumour activities of glucans and c. parvum.	after i.v. injection of glucans (yeast cell walls, lentinan and pseudonigeran) into cba mice, the peritoneal macrophages became cytostatic to r1 leukemia cells in vitro, and the mice showed resistance to lung nodule formation by i.v. injected t3 fibrosarcoma cells. the glucans were less active than c. parvum. none of the glucans reproducibly acted as an adjuvant when mixed with irradiated tumour cells; nor did they inhibit the growth of a subcutaneous tumour when injected i.v. or directly into t ...	1977	608513
effects of surgery and c. parvum on tumour metastases in mice.	surgical operations depress immune responses. the aim of this study was to observe the effects of operation (amputation) on the growth of the lewis lung tumour and its metastases and to see if c. parvum counteracted them. anaesthesia alone, or with amputation, did not affect the growth of the primary tumour but c. parvum depressed it. anaesthesia did not affect the number of pulmonary metastases but amputation caused significant increase. c. parvum inhibited metastases and completely counteracte ...	1977	608511
effects and mode of action of corynebacterium parvum on murine tumour metastases.	the effects of corynebacterium parvum (c. parvum) on lewis lung tumour metastases have been studied. i.v. and i.p., but not s.c. c. parvum significantly reduced pulmonary metastases. when combined with excision of the primary tumour, c. parvum given not more than 2 days before caused slight reduction of metastases, but when given earlier, it caused significant reduction and some mice were cured. metastases were increased by silica or cortisone acetate but were unaffected by trypan blue. the anti ...	1977	608510
in vitro anti-tumour properties of peritoneal exudate cells of conventional germ-free and stimulated mice.	adherent cells of peritoneal exudates were obtained from conventional, or from germ-free mice or from mice having received in intraperitoneal infection of a variety of phlogogenic substances such as corynebacterium parvum (c. parvum) (mérieux) 500 microgram, thioglycolate (difco) 3 ml, bayol (esso) 0.50 ml + 0.50 ml culture medium, glycogene 1.2 ml. the cytotoxic properties adherent cells were studied in vitro by the chromium release technique (crt) and their cytostatic properties by the inhibit ...	1977	608509
effect of corynebacterium parvum on the immune response of the guinea pig to ovalbumin.		1977	608508
potentiation of anamnestic humoral responses by a single intravenous injection of corynebacterium parvum (c. parvum) in adult rabbits.		1977	608507
cell mediated immunity to corynebacterium parvum and its role in tumor control.	the injection of c. parvum provokes a transient cell-mediated immune response to its own bacterial antigens. during this period a subcutaneous challenge dose results in the appearance of a characteristic delayed type hypersensitivity (dth) skin reaction. if a suspension of syngeneic tumor cells is included in this challenge, they are destroyed in immune but not control animals. similarly, solid tumors of limited size can be made to regress under these conditions. adoptive transfer studies reveal ...	1977	608505
monocytosis-inducing activity (mia) of serum in corynebacterium parvum treated mice.	intravenous injection of 548 microgram of c. parvum (lot 0407, mérieux laboratories, france) into c57bl mice produced rapidly appearing monocytopenia which was followed by marked and prolonged monocytosis after the third day. the serum of these animals, collected during the monocytopenic but not the monocytosis phase, showed monocytosis inducing activity (mia) as was demonstrated by the intravenous injection of the serum into normal test mice. serum from normal untreated mice or from mice given ...	1977	608504
antibody responses, antitumour antibodies and ig class and subclass levels in c. parvum treated mice.	this paper summarises the results of extensive studies performed in our laboratory during the past three years aimed at establishing the effect of c. parvum administration on humoral immunity in normal mice and mice previously challenged with viable mc fibrosarcoma cells. this treatment was found to (a) increase the response to thymus dependent and independent antigens, (b) result in the production of circulating immunoglobulins which bound to tumour cells and syngeneic tissues in vitro, and (c) ...	1977	608503
further studies on the enhancement of the antibody response in vitro by c. parvum.	we have shown previously that spleen cells from mice pre-treated with c. parvum give enhanced antibody response when cultured in the presence of thymus-dependent antigens. in order to establish if c. parvum has a similar effect when added in vitro, spleen cells from untreated mice were cultured with c. parvum in marbrook chambers with sheep erythrocytes (srbc) as antigen. at concentrations of 35-140 microgram/ml/20 x 10(6) spleen cells c. parvum enhanced, but at higher concentrations suppressed ...	1977	608502
distribution of 3h-thymidine-labelled c. parvum in mice.	little is known of the localisation of injected corynebacterium parvum. we therefore developed a method of radiolabelling this vaccine and determined its distribution in the mouse. live c. parvum (wellcome, strain cn 6134) was grown in the presence of 3h-thymidine and subsequently killed by formalin. a high activity, 1-6 x 10(5) cpm/0.1 ml of a 7 mg dry weight/ml concentrated suspension, was obtained. its biological properties (hepatosplenomegaly and antitumour effects) were similar to those of ...	1977	608501
distribution of technetium-99m labeled corynebacterium parvum in normal and tumor bearing mice.		1977	608499
studies using labelled c. parvum preparations in mice.	the in vivo distribution of 125i and fluorescein labelled c. parvum, after local and systemic injection mice has been studied. after i.v. injection large amounts were found in the liver, spleen and lungs, with less in the lymph nodes. the distribution after s. c. injection was more restricted; the bulk of inoculum was retained at the injection site and draining lymph node, and only small amounts were found in the liver and spleen. c. parvum was found in bone marrow, but not peritoneal cells afte ...	1977	608498
comparative studies on the cell wall composition of some anaerobic coryneforms of varying lympho-reticular stimulatory activity.	anaerobic diphtheroids possessing lympho-reticular stimulatory properties may differ considerably in their peptidoglycan composition. spleen weight-increasing activity of strains directly parallels their antitumour properties. p. granulosum strains, inactive in assays for lympho-reticular stimulation, appear to have a higher cell wall alanine content than most of the p. acnes and p. avidum strains tested. two p. acnes strains, however, had equivalently high alanine ratios and were stimulatory. t ...	1977	608497
the complications of intravenous corynebacterium parvum infusion.	100 intravenous infusions of corynebacterium parvum were given to thirty-six patients at a dose of 5 mg/m2. fever and rigors occurred in all patients but these acute side effects were acceptable to all but two patients. seventeen patients suffered a delayed fall in blood pressure, which was marked in eight of them but was generally well tolerated. one patient died 18 hr after infusion from extensive myocardial infarction. herpes labialis complicated the first infusion in nine instances, which ma ...	1977	606444
[immunological problems in cancer].		1977	601267
combination chemo-immunotherapy with adriamycin in experimental tumor systems.		1977	598392
effect of corynebacterium parvum on subcutaneous and skeletal methylcholanthrene sarcoma implants.		1977	598063
effect of corynebacterium parvum on peripheral blood platelets.	the level of peripheral blood platelets was determined after i.v. injection of corynebacterium parvum in normal c57bl mice and in those bearing the lewis lung carcinoma. twenty minutes after injection of a formalin-killed active strain (cn6134, (cn6134, which inhibited tumour metastases) or a killed inactive strain (cn 5888, which did not inhibit metastases) the number of circulating blood platelets was reduced by 50%. the level of platelets returned to control values by 8 h after the active, an ...	1977	597475
c. parvum immuntherapy of transplanted rat tumours.	c.parvum (wellcome cn 6134) has been tested for tumour suppression against a range of syngeneically transplanted rat tumours, both carcinogen-induced and of spontaneous origin. subcutaneous growth was not prevented by distant subcutaneous or intravenous injection of the preparation, although growth rates were sometimes depressed or accelerated. in contrast, c. parvum injected in admixture with tumour cells consistently suppressed their growth and with highly immunogenic tumours induced systemic ...	1977	591131
susceptibility to erythromycin of anaerobes of the genera bacteroides, fusobacterium, sphaerophorus, veillonella, clostridium, corynebacterium, peptococcus, peptostreptococcus.	the minimal inhibitory concentrations (mic) of erythromycin were determined by broth dilution tests for 313 anaerobic strains, most of which were clinical isolates. all the gram-positive anaerobes tested (84 peptococcaceae, including 21 peptostreptococcus anaerobius and 15 peptococcus variabilis; 65 corynebacterium acnes and 29 clostridium strains, including 13 c. perfringens) were sensitive (mic values 0.012 through 3.12 microgram erythromycin/ml); so were 111 cultures of gram-negative anaerobe ...	1977	580038
active experimental inhibition of cancer by corynebacterium parvum.	it has been demonstrated that 5 microgram corynebacterium parvum (mérieux, lyon) applied i.v. once weekly in mice of the strain c 3 h x swiss albino f 1 after three pregnancies effectively inhibits tumour growth. at this time those mice are 100% at risk. the appearance of tumours in the treated group is significantly delayed.	1977	579449
use of litmus milk agar for presumptive identification of cutaneous propionibacteria.	presumptive identification of cutaneous propionibacteria can be achieved with litmus milk agar as medium.	1977	577927
[antitumor effect of corynebacterium parvum].	the protective effect produced by pre-treatment with c. parvum is studied in two syngeneic tumours: mammary carcinoma in c3h mice and lymphosarcoma in xvii mice. the best anti-tumour effect is obtained when both c. parvum and tumour cells are injected by the same route, which, however varies for the two tumours. the study of the recovery of c. parvum protective effect after its suppression by a sub-letal dose of x-irradiation shows that, in the c3h mice, it is parallel to the restoration of the ...	1977	576780
[anerobic corynebacteria propionibacterium as immunostimulators and antioncogenic factors].		1977	564299
corynebacterium parvum as an immunotherapeutic agent in an ovarian cancer model.	corynebacterium parvum, an anaerobic diptheroid, has been demonstrated to be therapeutic in several tumor models by stimulating immunologic defenses. formalin-killed c. parvum was investigated in the present study as an immunotherapeutic agent in the treatment of murine ovarian cancer, a model that closely simulates the activity of clinical disease. c. parvum successfully prolonged survival in murine ovarian cancer and its effectiveness improved with increasing dosage. the efficacy of c. parvum ...	1977	560124
[in vitro sensitivity of corynebacterium acnes (propionibacterium acnes) to meclocycline, clindamycin, erythromycin and neomycin].		1979	554810
role of mononuclear phagocytes in decreased hepatic drug metabolism following administration of corynebacterium parvum.		1979	551956
activation of pleural macrophages by intrapleural application of corynebacterium parvum.	a single ipl injection of 0.25 mg cp into cba mice led to accumulation of macrophages in the pleural cavity, but it did not influence res as an injection given iv ipl cp caused a three-to-five-fold increase in the number of nucleated cells in the pleural cavity which persisted at least 14 days. of these cells 86% were macrophages as shown by their esterase activity. less than 30% of cells from the pleural cavity of normal mice were esterase positive. macrophages from the pleural cavity of cp-tre ...	1979	547729
protection of mice against endotoxin-induced liver damage by anti-inflammatory drugs.	mice were injected with corynebacterium parvum, which induces multiple granulomas in liver and renders animals hyper-reactive to the lethal effect of bacterial lipopolysaccharide (lps). such animals when challenged with lps developed also extensive liver parenchymal cell damage as estimated by elevated blood asparate transaminase levels and a hypoglycaemia. treatment with indomethacin, hydrocortisone, dexamethasone, promethazine, metiazinic acid and (+)-catechin ameliorated the liver damage. hyd ...	1979	546179
comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline.	the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ...	1979	543353
prolonged effect of corynebacterium parvum stimulation on granulopoiesis.	an intravenous injection of 548 microgram of killed corynebacterium parvum (c. parvum) into c57bl mice led to a significant increase in the number of both committed stem cells (gm-cfc) and proliferating cells of bone marrow granulocytic series. this increase appeared 24 h after the injection of the bacterium, persisted in varying levels until the 4th week and was accompanied by a marked and prolonged granulocytosis. the proportion of gm-cfc in dna synthesis was found to be increased 1, 2, and 5 ...	1979	542797
growth of cutaneous propionibacteria on synthetic medium; growth yields and exoenzyme production.		1979	541303
effects of intravenous injection of two different strains of corynebacterium parvum in the mouse.	a strain of c. parvum, cn6134, known to have antitumour activity, caused thrombosis in the sites where the organism is phagocytosed. it bound to macrophages in vitro and activated the alternate pathway of complement. a strain of c. parvum, cn5888, which fails to show antitumour activity, did not show thrombosis. it did not bind to macrophages or activate guinea-pig complement. it did, however, cause marrow infarction which seems to result from a diminished clearance of the organism from the circ ...	1979	540104
biotyping of propionibacterium acnes isolated from normal human facial skin.	biochemical and serological characteristics of 128 strains of propionibacterium acnes isolated from the facial skin of healthy japanese volunteers were compared with the three standard strains of the american type culture collection, atcc 6919, 11827, and 11828. accordingly, the isolated strains of p. acnes were classified into five biotypes (b1 to b5) on the basis of fermentation tests of ribose, erythritol, and sorbitol. two serotypes were distinguished by the agglutination test. p. acnes belo ...	1979	539817
action of cancer chemotherapy-radiotherapy on spleen hypertrophy response to corynebacterium parvum adjuvant therapy.		1979	536279
active, specific immunotherapy of murine leukemia. ii. adjuvant effect of corynebacterium parvum.	we have previously shown that irradiated lstra cells (lx) were immunogenic and could prolong survival of mice bearing lstra tumors. this study demonstrated that addition of corynebacterium parvum to the lx dramatically improved the strength of tumor immunity. in pretreatment-challenge experiments, c. parvum augmented the immunogenicity of 10(6) lx given intradermally, with an optimum dose of 0.14-1.4 microgram c. parvum per mouse. in therapy experiments (intraperitoneal vaccine treatment after t ...	1979	528079
the facilitation of tumour growth in the lung by cyclophosphamide in artificial and spontaneous metastases models.		1979	527634
chemical and biological properties of a purified lymphoreticular-stimulating fraction of corynebacterium parvum (propionibacterium acnes type i).	a method is described in which washed whole cells of corynebacterium parvum were chemically and enzymatically extracted to remove cytoplasm and cell-wall lipids. the resultant insoluble cell-wall residue possessed lympho-reticular stimulating properties as measured by their ability to increase spleen weight and protect against tumour-cell challenge. analysis of the final product by chromatography and infrared spectroscopy has shown it to consist of carbohydrate and peptidoglycan, both of which a ...	1979	513108
alterations induced in macrophage and granulocyte-macrophage colony-forming cells by a single injection of mice with corynebacterium parvum.		1979	513059
macrophage precursors in ovarian carcinoma.		1979	510998
[adriamycin-combination chemotherapy with or without immune stimulation by corynebacterium parvum in metastasizing carcinoma of the breast (author's transl)].	in a randomised, multicentre study the effect of changing the combined cytostatic treatment with vac (vincristin, adriamycin, cyclophosphamide) to fmc (fluorouracil, methotrexate, cyclophosphamide) was tested, as well as the effect of active non-specific immune stimulation with corynebacterium parvum, and compared with a control group. in 59 of 120 patients (49%) in whom the results could be analysed there was a measurable significant regression of tumour size to less than 50% of its initial val ...	1979	510206
host treatments affecting artificial pulmonary metastases: interpretation of loss of radioactively labelled cells from lungs.	the effect has been examined of various host treatments (c. parvum injection, immunization, thoracic irradiation, cyclophosphamide injection, and anticoagulation) on both lung colony formation and clearance of radioactive cells from the lungs after i.v. injection of tumour cells. two tumour-host models have been used: the non-immunogenic kht tumour in c3h/km mice, and the immunogenic emt6 tumour in balb/c/ka mice. even for the at most weakly immunogenic kht tumour, the number of artificial pulmo ...	1979	508577
lung cancer patients treated with corynebacterium parvum. phase i toxicity study.		1979	503003
effects of whole body irradiation in mice treated with c. parvum.		1979	499277
surgery plus corynebacterium parvum immunotherapy for lewis lung carcinoma in mice.		1979	499275
comparative antitumor effects of corynebacterium parvum, bordetella pertussis, bacillus calmette-guérin, and levamisole alone or in combination with cyclophosphamide in the cad2 murine mammary adenocarcinoma system.	the antitumor efficacy of various immune stimulants [corynebacterium parvum, bordetella pertussis, and bacillus calmette-guérin (bcg)] and levamisole alone or in conjunction with cyclophosphamide (cy) was studied in the cad2 mammary adenocarcinoma system using schedules developed previously with c. parvum and cy. weekly systemic treatment with c. parvum, b. pertussis, or bcg was effective in controlling tumor growth and had equivalent antitumor effects, but weekly treatment (or a single treatmen ...	1979	498111
pharmacokinetics of phenytoin and liver cytochrome p-450 levels in mice after single doses of corynebacterium parvum [proceedings].		1979	497584
anaerobic bacteria in late infections following orthopedic surgery.	twelve patients with late anaerobic infection following hip or knee arthroplasty or tibia osteosynthesis are presented. peptococci (peptococcus asaccharolyticus, p. magnus, p. prevotii) and peptostreptococci (peptostreptococcus micros, p. anaerobius, p. parvulus) were present in nine of the cases. anaerobic gram-positive or -negative rods were found in three cases (propionibacterium acnes, actinomyces israelii, and bacteroides fragilis). antibodies against the isolated bacteria were detected in ...	1979	492071
chemoimmunotherapy for meningeal melanocytoma of the thoracic spinal cord. report of a case.		1979	490860
immunotherapy of a murine ovarian carcinoma with corynebacterium parvum and specific heteroantiserum. i. activation of peritoneal cells to mediate antibody-dependent cytotoxicity.	immunotherapy with a combination of corynebacterium parvum and specific heteroantiserum is significantly more effective than treatment with either single agent in prolonging the survival of mice that have recevied an i.p. injection of syngeneic murine ovarian carcinoma (mot) cells. invitro, a combination of c. parvum-activated peritoneal cells and specific heteroantiserum has proven significantly more effective than either single component in destroying 51cr-labeled mot cells in the absence of c ...	1979	489967
the reversal of surgically induced reticuloendothelial depression.	the function of the reticuloendothelial (re) system may be depressed by surgical operation. as this system is an important defence mechanism against tumour growth and metastasis, it would be an advantage if this depression could be prevented by the administration of a suitable agent. the agents studied in this case were levamisole, corynebacterium parvum (c. parvum) and glucan. as a measure of re function a carbon clearance test was performed in rats, 1 hr after partial colectomy, animals having ...	1979	487654
effects of repeated corynebacterium parvum and bcg therapy on immune parameters: a weekly study of melanoma patients ii. changes in serum immunoglobulins and lymphoid cell subpopulations.	two groups, each of eight patients, with metastatic melanoma were immunized with either c. parvum or bcg at 3-weekly intervals. the serum igg, iga, igm levels, e-rosettes, ea-rosetts, sig-bearing cells, 'null cells' and lymphocyte blastogenesis at four pha concentrations were assayed, before immunization and weekly thereafter for 8 weeks. the pre-immunization e-rosette values were significantly reduced, with a significant increase in 'null cell' values and ea-rosette percentages, when compared w ...	1979	487647
fc receptor-bearing and phagocytic cells in syngeneic tumours of c. parvum- and carrageenan-treated mice.		1979	486317
monocytes and macrophages in malignant melanoma iv. effects of c. parvum on monocyte function.	assays for the capacity of peripheral-blood monocytes (a) to mature in vitro into macrophages, (b) to reduce nitro-blue tetrazolium (nbt) and (c) to lyse antibody-coated human group a red cells, were applied to a group of 82 patients with histologically proven malignant melanoma. in patients with micrometastatic disease there was an enhancement of red-cell lysis and nbt reduction, suggesting that their monocytes are in some way "activated", whereas nbt reduction was suppressed in those with over ...	1979	486312
activation of human monocytes by mediators from lymphocytes stimulated with corynebacterium parvum.	human monocytes activated in vitro by lymphokine-containing supernatants of autologous or allogeneic lymphocytes stimulated in vitro by corynebacterium parvum (cp) expressed increased ability to suppress dna-synthesis in a human tumour cell line. monocyte activation was not dependent on in vitro differentiation of monocytes, enhanced cytostatic ability being observed at all stages of in vitro differentiation. the lymphokine-induced cytostatic ability was not affected by intensive washing and try ...	1979	484237
non-specific and specific active immunotherapy in a b16 murine melanoma system.	conflicts amongst reports concerning the efficacy of both nonspecific and specific attempts at immunotherapy may be ascribed to different animal models utilizing tumors of different immunogenicity. we have selected the b16 mouse melanoma model as the example of a spontaneously occurring neoplasm that is histocompatible with the host and does have tumor-associated antigens. attempts to alter tumor growth or survival with nonspecific active immunotherapy as well as with specific active immunothera ...	1979	480953
dual effect of normal and stimulated macrophages and their conditioned media on target cell proliferation.		1979	480375
comparison of the in-vitro antitumor activity of peritoneal cells from mice injected with corynebacterium parvum and corynebacterium liquefaciens.		1979	478988
propionibacterium acnes pneumonia in a patient with lymphoma.	a 57 year-old male with lymphocytic lymphoma developed symptoms of cough, fever and progressive dyspnea accompanied by a diffuse lower lung infiltrate. open lung biopsy revealed multiple micro-abscesses surrounding small bronchioles which yielded a pure growth of propionibacterium acnes.	1979	478654
[effect of corynebacterium parvum on serum lysozyme (muramidase) levels (author's transl)].	an i.v. injection of 548 microgram of killed corynebacterium parvum into c57b1 mice leads to significant changes in serum lysozyme (muramidase) levels. after an initial fall at 24 h, the activity of the enzyme increased progressively, reached a peak on the 9th day and returned to control range after the 15th day.	1979	477851
enhancement of the thermal response of animal tumors by corynebacterium parvum.	the effect of corynecbacterium parvum treatment on the thermal response of animal tumors was studied. tumors were methylcholanthrene-induced (fsa-ii) and spontaneous (fsa-i) fibrosarcomas in c3hf/sed mice. c. parvum was given i.v. and was followed by local hyperthermia at 43.5 degrees 3 days later. cell survival determined by lung colony assays showed that preadministration of c. parvum insignificantly enhanced the thermal response of both tumors. studies of delay of tumor growth for fsa-ii demo ...	1979	476675
sensitivity to chemotherapeutic and immunomodulating agents of two mouse lymphomas and of a macrophage tumor.	the effect of treatment with 15 chemotherapeutic and 10 immunomodulating agents on the growth of t-cell lymphoma el-4, macrophage tumor j774, and b-cell lymphoma 70z/2 of the mouse has been studied using the prolongation of median survival time of tumor-bearing hosts as an index of therapeutic effectiveness. the survival time of mice bearing 70z/2 was prolonged more than 100% by single-agent therapy with actinomycin d, cyclophosphamide, 6-mercaptopurine, mitomycin c, and vinblastine; a similar r ...	1979	476634
inhibition of spontaneous akr leukemia by multiple inoculations of corynebacterium parvum.	in previous studies we have shown that akr mice could be protected from spontaneous leukemia by specific immunotherapy in combination with splenectomy. in this experiment we investigated the effects of nonspecific immunotherapy with c. parvum in a similar regimen. it was found that bi-weekly, ip inoculations of 0.7 mg of c. parvum could significantly protect akr mice from spontaneous tumors, and that splenectomy could not modify this effect. thus, the spleen does not appear to play a determinant ...	1979	476565
an ultrastructural study of peritoneal mononuclear phagocytes from corynebacterium parvum-injected mice.	peritoneal mononuclear phagocytes harvested from mice 24 h after i.p. injection of c. parvum displayed hypertrophy of the golgi and smooth endoplasmic reticulum complex with attendant increase in lysosome production. the ingested bacilli were identified within phagolysosomes, within which large myelin figures accumulated. in addition to heterophagic vacuoles, autophagosomes and lipid droplets were observed. these latter two inclusions may reflect a direct or indirect cytopathic effect of c. parv ...	1979	475974
[equal sensitivity of normal or tumoral fibroblasts to cytotoxic and cytostatic effects of normal or "corynebacterium parvum" activated peritoneal exudate cells (author's transl)].	cytotoxic and cytostatic properties of peritoneal exudate cells from balb/c mice either normal or pretreated with corynebacterium parvum normal were studied using as target cells two lines of balb/c fibroblasts one normal (balb) and one transformed by kirsten virus (k.balb).. c. parvum activated cells displayed higher cytotoxic as measured by 51cr or 3h-thymidine release and cytostatic, as measured by 3h-thymidine incorporation, activities against these lines of syngeneic cells. cytotoxic effect ...	1979	475346
choriogonadotropin-like antigen in an anaerobic bacterium, eubacterium lentum, isolated from a rectal tumor.	using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of eubacterium lenthum that was originally isolated from a rectal tumor. in contrast, both immunohistochemical reactions were negative when applied to a strain of co ...	1979	468380
regression of regionally advanced melanoma after arterial infusion with cis-platinum and actinomycin-d.		1979	466896
is there postdefecation bacteremia?	an investigation was done to determine the incidence of postdefecation bacteremia. a study of 82 healthy volunteers was done. among 164 predefecation cultures, only one (0.6%) from an anaerobic flask was positive for microorganism, which was identified as staphylococcus epidermidis. in only two of 328 (0.6%) postdefecation cultures did microorganisms grow, which were shown to be propionibacterium acnes. the results of this study suggest that either bacteremia after defecation does not occur or i ...	1979	464809
investigations on the bacteriostatic effect of benzoyl peroxide.		1979	464656
effect of corynebacterium parvum in prevention and reversal of atrophy of the liver following portacaval shunt.	since it has been demonstrated that the administration of corynebacterium parvum is associated with increased hepatocyte proliferation in both normal and regenerating livers, it seemed appropriate to determine whether the use of this agent would affect the hepatic atrophy which occurs following end-to-side portacaval shunts. when administered at shunting, liver weight and liver deoxyribonucleic acid failed to decrease to the same extent as that occurring in untreated, shunted rats. an increase i ...	1979	462347
[postoperative prevention of recurrence with intrapleural corynebacterium parvum in patients with stage i and ii bronchial carcinomas. ludwig lung cancer study i].	the ludwig lung cancer group was created in 1977. participants are from austria, denmark, germany, norway, sweden, switzerland, and yugoslavia. 400 patients are randomized yearly. the clinical trial i investigating the role of c. parvum intrapleurally as adjuvant therapy in operable non-small cell lung cancer patients has been closed in february 1979 with a total accrual of 475 patients. the average follow-up time for these cases is approximately 8 months. it is early to make any definitive comp ...	1979	462160
[prospects of short-term sequential immunomodulation with levamisole and corynebacterium parvum in neoplastic pathology in the aged].		1979	460632
quantitative and functional differences induced in bone marrow-derived mononuclear phagocytes by inflammatory stimuli.		1979	458783
macrophage activation and increased resistance to infection in immunosuppressed mice treated with corynebacterium parvum vaccine or pyran copolymer.		1979	458782
positive correlation between the levels of natural killer cells and the in vivo resistance to syngeneic tumor transplants as influenced by various routes of administration of corynebacterium parvum bacteria.		1979	455483
effect of 1(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (acnu) on sato lung carcinoma (slc). preliminary result of immunochemotherapy for slc by combination of acnu and propionibacterium acnes.	acnu was highly effective for sato lung carcinoma transplanted intravenously or intramuscularly by using a large single dose, and the cytotoxic action of acnu for slc showed clear dependence upon tumor size. nonspecific activation of host-defence mechanism by propionibacterium acnes contributed to the suppression for the regrowth of solid slc treated by acnu.	1979	450390
chemoimmunotherapy in the management of primary stage iii ovarian cancer: a gynecologic oncology group study.	the effectiveness of chemoimmunotherapy was evaluated in 45 previously untreated stage iii ovarian cancer patients. response rate, progression-free interval, and survival were considerably better than in a similar group of 63 patients treated with a single alkylating agent alone.	1979	445505
immunotherapeutic response of concanavalin a-bound l1210 vaccine enhanced by a streptococcal immunopotentiator, ok-432.	immunotherapeutic response to concanavalin a (con a)-bound l1210 murine leukemic vaccine and immunopotentiators was examined in histocompatible animals bearing a small burden l1210 leukemic cells. when combined with con a-bound vaccine, a streptococcal immunopotentiator, ok-432 (nsc b116209), prepared from streptococcus pyogenes, was potent in antitumor therapy and resulted in a number of cured animals. administration of either con-a-bound vaccine or ok-432 alone did not produce any beneficial e ...	1979	445486
metabolic changes following the intravenous infusion of corynebacterium parvum in man.	the acute changes in concentrations of key blood metabolites and liver function tests were measured following intravenous infusion of corynebacterium parvum in 9 healthy patients who had recently undergone resection of a colorectal cancer. the following results were obtained: 1) blood glucose, lactate and ketone body concentrations significantly increased over a 5 hour study period; 2) blood alanine fell during the same period; 3) plasma bilirubin, got and urea were significantly elevated 24 hou ...	1979	445333
lack of effect of immunotherapy with bcg and corynebacterium parvum on hepatic drug hydroxylation in man.	serial serum diphenylhydantoin and urinary 5-(p-hydroxphenyl)-5-phenylhydantoin concentrations were determined in 8 patients with malignant disease and 4 healthy volunteers on 2 separate occasions after an oral dose of diphenylhydantoin (500 mg). no significant difference was observed between metabolism before and 10 days after immunization with bcg or corynebacterium parvum. volunteers without intervening immunization similarly showed no difference.	1979	444399
[porphyrin production of propionibacterium acnes].		1979	442749
aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study.	aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. the cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. the conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric co ...	1979	440845
laboratory induction and clinical occurrence of combined clindamycin and erythromycin resistance in corynebacterium acnes.	corynebacterium acnes strains cross-resistant to clindamycin and erythromycin were observed following long-term selection or mutagenic treatment in the laboratory. similar strains were found among clinical isolates from patients using clindamycin or erythromycin topically in the treatment of acne vulgaris. clindamycin resistance was never observed in the absence of resistance to macrolides or other lincosaminides. it is suggested that this resistance may result from an alteration of the 50s ribo ...	1979	429800
fatal infection with pseudomonas aeruginosa in mice treated with cyclophosphamide and propionibacterium acnes.		1979	428451
effects of corynebacterium parvum and bcg therapy on immune parameters in patients with disseminated melanoma a sequential study over 28 days. i. changes in blood counts, serum immunoglobulins and lymphoid cell populations.	the effects of a single immunization of melanoma patients with bcg or c. parvum on the blood counts, serum immunoglobulin levels and lymphoid subpopulations were followed by multiple assays over 28 days. c. parvum produced a decrease in the white cell count, lymphocyte count and lymphoid t and sig+ cell numbers, which recovered within 1 week; bcg did not produce such a marked depression. both agents were associated with increases in t cell numbers and lymphocyte pha blastogenesis after the first ...	1979	428146
conditions for effective bacillus calmette-guérin immunotherapy of postsurgical metastases of 13762a rat mammary adenocarcinoma.	we evaluated critical variables in bacillus calmette-guérin (bcg) immunotherapy of residual 13762a rat mammary adenocarcinoma. bcg was given intratumorally on day 7 of tumor growth and followed by primary tumor excisions on day 20. untreated animals died on about day 40 with axillary nodal and pulmonary parenchymal metastases. bcg-treated animals experienced prolonged survival, and some were cured. the highest dose (5.0 x 10(7) colony-forming units) of bcg was more effective than the lowest (0.5 ...	1979	427785
the aerobic growth of propionibacterium acnes in primary cultures from skin.	although propionibacterium acnes ordinarily grows as an anaerobe, this organism was commonly seen on aerobic primary cultures (directly from skin) of the forehead, axilla, deltoid area, and antecubital fossa. on subculture, strains from aerobic primary cultures showed their normal anaerobic state. on repeated tests of the foreheads of 7 subjects over periods of 1 to 4 yr, 24% of all cultures were positive for propionibacteria (p. acnes) on aerobic culture. this phenomenon was equally common in c ...	1979	422866