Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| catalytic and structural properties of irt-21 beta-lactamase (tem-77) from a co-amoxiclav-resistant proteus mirabilis isolate. | proteus mirabilis strain mag1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant tem (irt)-21, a novel mutant of tem beta-lactamase. this enzyme has a pi of 5.2 and is derived from the bla(tem-1a) gene ancestor. it contains two major amino acid substitutions specific for co-amoxiclav resistance (leu-69 for met and ser-244 for arg) that have never been found together previously. the dramatic loss of sensitivity to clavulanic acid, th ... | 2001 | 11750800 |
| minipodia and rosette contacts are adhesive organelles present in free-living amoebae. | using scanning electron microscopy, amoeba proteus cells migrating on the glass have been shown to develop dense coats of minipodia, which are discrete microprotrusions up to 8 microm long and approximately 0.5 microm across. they cover the middle-anterior area of the ventral cell surface, i.e. the region previously determined as the zone of most efficient adhesion of an amoeba to its substratum. minipodia are sparse underneath the frontal zone and lacking from the tail region. in amoebae that a ... | 2001 | 11748921 |
| a novel germline mutation of the pten gene in a patient with macrocephaly, ventricular dilatation, and features of vater association. | mutations of the pten gene are associated with hamartoma-neoplasia syndromes. while germline mutations at this chromosome 10q22-23 locus have been observed in patients with cowden syndrome (cs) and bannayan-riley-ruvalcaba syndrome (brr), both of which phenotypes are associated with hamartomata and neoplasia, somatic mutation of pten has been established in a wide variety of sporadically occurring neoplasia. cs and brr share some clinical features, specifically hamartomata and lipomatosis. inves ... | 2001 | 11748304 |
| visualization of proteus mirabilis within the matrix of urease-induced bladder stones during experimental urinary tract infection. | the virulence of a urease-negative mutant of uropathogenic proteus mirabilis and its wild-type parent strain was assessed by using a cba mouse model of catheterized urinary tract infection. overall, catheterized mice were significantly more susceptible than uncatheterized mice to infection by wild-type p. mirabilis. at a high inoculum, the urease-negative mutant successfully colonized bladders of catheterized mice but did not cause urolithiasis and was still severely attenuated in its ability to ... | 2002 | 11748205 |
| sonographic detection of multiple brain abscesses in a newborn with iga deficiency. | we report the case of a neonate with selective iga deficiency and multiple brain abscesses diagnosed with sonography. brain sonography revealed multiple abscesses in the left hemisphere; the abscesses ranged from 10 to 20 mm. cultures obtained from the cerebrospinal fluid and blood were positive for proteus mirabilis. the neonate responded promptly to broad-spectrum antibiotic therapy and had no neurologic sequelae. because iga deficiency is associated with infections, we believe it was a predis ... | 2001 | 11745857 |
| retrospective study of children with acute pyelonephritis. evaluation of bacterial etiology, antimicrobial susceptibility, drug management and imaging studies. | the purpose of the study was to investigate the management of pyelonephritis in a large italian pediatric population. a total of 1,333 patients (36% male) were considered. escherichia coli was the most frequently isolated agent (89.9%), followed by proteus mirabilis (3.6%) and klebsiella oxytoca (2.1%). 27% of microorganisms were resistant to amoxicillin, 4% to amoxicillin/clavulanic acid, 11% to trimethoprim-sulfamethoxazole, 2.4% to gentamicin and less than 2% to ceftazidime. despite this resi ... | 2002 | 11744799 |
| bilateral septic arthritis of the knee joint in three children caused by unusual infectious agents. | 2001 | 11737753 | |
| chaotic behavior in the locomotion of amoeba proteus. | the locomotion of amoeba proteus has been investigated by algorithms evaluating correlation dimension and lyapunov spectrum developed in the field of nonlinear science. it is presumed by these parameters whether the random behavior of the system is stochastic or deterministic. for the analysis of the nonlinear parameters, n-dimensional time-delayed vectors have been reconstructed from a time series of periphery and area of a. proteus images captured with a charge-coupled-device camera, which cha ... | 2001 | 11732198 |
| gut-derived bone infection in the neonatal rat. | the risk of osteomyelitis is increased in the premature and critically ill neonate. although potential sites of bacterial entry are present in many of these infants, the source of infection frequently cannot be established. this study was performed to assess the possible role of bacterial translocation from the intestine in the origin of bone infection using models of breast-fed and formula-fed rat pups. newborn sprague-dawley rats suckled either ad libitum by the dam (n = 30), or were fed a rat ... | 2001 | 11726738 |
| purification and characterization of the groeslx chaperonins from the symbiotic x-bacteria in amoeba proteus. | groelx and groesx proteins of symbiotic x-bacteria from amoeba proteus were overproduced in escherichia coli transformed with pajx91 and puxgprm, respectively, and their chaperonin functions were assayed. we utilized sigma(70)-dependent specific promoters of groex in the expression vectors and grew recombinant cells at 37 degrees c to minimize coexpression of host groe of e. coli. for purifying the proteins, we applied the principle of heat stability for groelx and pi difference for groesx to mi ... | 2001 | 11722184 |
| structure of the o-specific polysaccharide of proteus vulgaris o37 and close serological relatedness of the lipopolysaccharides of p. vulgaris o37 and p. vulgaris o46. | the o-specific polysaccharide (o-antigen) of the lipopolysaccharide (lps) of proteus vulgaris o37 was studied by (1)h and (13)c nuclear magnetic resonance spectroscopy before and after o-deacetylation and found to be structurally similar to that of p. vulgaris o46 studied earlier. the two polysaccharides have the same carbohydrate backbone and differ in the position and number of the o-acetyl groups only. studies with o-antisera against the two strains using passive hemolysis test, enzyme immuno ... | 2001 | 11720819 |
| ssu rrna-based phylogenetic position of the genera amoeba and chaos (lobosea, gymnamoebia): the origin of gymnamoebae revisited. | naked lobose amoebae (gymnamoebae) are among the most abundant group of protists present in all aquatic and terrestrial biotopes. yet, because of lack of informative morphological characters, the origin and evolutionary history of gymnamoebae are poorly known. the first molecular studies revealed multiple origins for the amoeboid lineages and an extraordinary diversity of amoebae species. molecular data, however, exist only for a few species of the numerous taxa belonging to this group. here, we ... | 2001 | 11719580 |
| [heat shock response in experimental acute middle ear bacterial infection]. | to investigate the association between bacteria antigen and inner ear antigen and effects of stress on the expression spectrum and level of the antigen bearing hsp70 related epitope. | 1998 | 11717868 |
| pattern of gram negative rods bacteraemia in diabetic patients in ibadan, nigeria. | in a study of 100 patients in ibadan between july and december 1995 to evaluate bacteraemia due to gram-negative bacilli, 64% were culture positive, 44 (68.8%) of these yielded gram-negative rods. the isolates were klebsiella species (43.2%), escherichia coli (27.3%), pseudomonas aeruginosa (13.6%), proteus species (11.4%) and bacteroides melaninogenious (4.15%) by standard bacteriological methods. antimicrobial sensitivity results suggested ofloxacin or ceftriaxone with metronidazole as empiric ... | 2000 | 11713990 |
| tem-89 beta-lactamase produced by a proteus mirabilis clinical isolate: new complex mutant (cmt 3) with mutations in both tem-59 (irt-17) and tem-3. | tem-89 (cmt-3) is the first complex mutant beta-lactamase produced by a clinical strain of proteus mirabilis (strain pm 631). this new enzyme, which has a pi of 6.28, is derived from tem-3 and has a single amino acid substitution also encountered in tem-59 (inhibitor-resistant tem beta-lactamase irt-17): ser-130 to gly. tem-89 hydrolyzed penicillins to the same extent that tem-3 did but lost almost all hydrolytic activity for cephalosporins and, like tem-59, was highly resistant to inhibitors. | 2001 | 11709345 |
| cefepime, piperacillin-tazobactam, and the inoculum effect in tests with extended-spectrum beta-lactamase-producing enterobacteriaceae. | there is little information about the clinical effectiveness of cefepime and piperacillin-tazobactam in the treatment of infections caused by extended-spectrum beta-lactamase (esbl)-producing pathogens. some inferences have been drawn from laboratory studies, which have usually involved only one or a few strains of esbl-producing klebsiella pneumoniae or escherichia coli that produced only a limited range of esbls. such studies are indirect, sometimes conflicting, indicators of efficacy. to exte ... | 2001 | 11709338 |
| urinary excretion and bactericidal activities of gemifloxacin and ofloxacin after a single oral dose in healthy volunteers. | in a randomized crossover study, 16 volunteers (8 men, 8 women) received single oral doses of 320 mg of gemifloxacin and 400 mg of ofloxacin on two separate occasions in the fasting state to assess the urinary excretion and urinary bactericidal titers (ubts) at intervals for up to 144 h. ofloxacin showed higher concentrations in urine compared with those of gemifloxacin. the median (range) cumulative excretion of gemifloxacin was 29.7% (8.4 to 48.7%) of the parent drug administered, and median ( ... | 2001 | 11709334 |
| microbial, chemical and physical aspects of citrus waste composting. | citrus waste supplemented with calcium hydroxide and with a c/n ratio of 24:1, ph of 6.3 and moisture content of 60% was composted by piling under shelter. with regular turning over of the pile and replenishment of moisture, the thermic phase lasted for 65-70 days and composting was completed after 3 months. compost thus prepared had an air-filled porosity of 14%, water-holding capacity of 590 ml l(-1), bulk density of 1.05 g cm(-3) and conductivity of 480 ms m(-1). phosphorus content (in mg l(- ... | 2002 | 11708757 |
| predominant bacterial agents of childhood septicaemia in jos. | blood cultures were performed on 891 children (aged 1 month-16 years) who presented at the jos university teaching hospital (juth) with presumed diagnosis of septicaemia, over a three year period (january 1994-december 1996) in order to determine the predominant bacterial agents. one hundred and thirty-nine (15.6%) out of 891 blood cultures were positive. five of the blood cultures yielded double isolates. staph. aureus was the commonest bacterial agent isolated and accounted for 36.0%, of all t ... | 2001 | 11705063 |
| proteus syndrome associated with ocular anomalies. | 2001 | 11703003 | |
| examination of pineal indoles and 6-methoxy-2-benzoxazolinone for antioxidant and antimicrobial effects. | oxidative modification of low density lipoprotein (ldl) induced by free radicals is implicated in the development of atherosclerosis. the aim of the present study was to examine the ability of various pineal indoles in inhibiting ldl oxidation which is accompanied by an increase in mobility in agarose gel electrophoresis and by an augmented generation of thiobarbituric acid-reactive substance induced by cu2+. it was found that the order of potencies in inhibiting malondialdehyde formation was 5- ... | 2001 | 11701394 |
| analyses of mrp genes during myxococcus xanthus development. | myxococcus xanthus is a gram-negative soil bacterium that undergoes development under starvation conditions. our previous study identified a new genetic locus, mrp, which is required for both fruiting body formation and sporulation. the locus encodes two transcripts: mrpab, which consists of a histidine kinase and an ntrc-like response regulator, and mrpc, a cyclic amp receptor protein family transcription activator. in this study, we used genetic and biochemical analyses to investigate the poss ... | 2001 | 11698359 |
| cellular membrane associated mucins in artificial urine as mediators of crystal adhesion: an in vitro enterocystoplasty model. | cells are a major component of mucus in enterocystoplasties. we evaluate the role of secreted mucins on cells and cellular membranes as crystal adhesion and agglomeration mediators in artificial urine infected with proteus mirabilis. | 2001 | 11696777 |
| formation of a tyrosyl radical intermediate in proteus mirabilis catalase by directed mutagenesis and consequences for nucleotide reactivity. | proteus mirabilis catalase (pmc) belongs to the family of nadph binding catalases. the function of nadph in these enzymes is still a matter of debate. this study presents the effects of two independent phenylalanine mutations (f194 and f215), located between nadph and heme in the pmc structure. the phenylalanines were replaced with tyrosines which we predicted could carry radicals in a nadph-heme electron transfer. the x-ray crystal structures of the two mutants indicated that neither the bindin ... | 2001 | 11695923 |
| evaluation of extracts of the root of landolphia owerrience for antibacterial activity. | ethanolic and aqueous (cold and hot) extracts of landolphia owerrience root parts (whole-root, root-bark and root-wood) were tested for activity against ten bacterial strains using agar-well diffusion and macro-broth dilution methods, respectively. the ethanolic extracts of the whole-root and root-wood were active against 100 and 80% of the test organisms, respectively. ethanolic and aqueous extracts of the root-bark were moderately active while the aqueous (cold and hot) extracts of the root-wo ... | 2001 | 11694355 |
| epidermal naevus and segmental hypermelanosis associated with an intraspinal mass: overlap between different mosaic neuroectodermal syndromes. | the epidermal naevus syndrome is a neurocutaneous syndrome characterised by the association of epidermal naevi with central nervous system, skeletal, ocular or cardiovascular abnormalities. we report on a 1.75-year-old boy who presented with spastic diparesis, a partial paresis of the left forearm and macrocephaly. he had a large epidermal naevus along the cervical spine and a segmental hypermelanosis. mri studies revealed a large intraspinal mass extending from the lower cervical to the upper t ... | 2001 | 11686504 |
| a european perspective on nosocomial urinary tract infections i. report on the microbiology workload, etiology and antimicrobial susceptibility (esgni-003 study). european study group on nosocomial infections. | to obtain information on the microbiology workload, etiology and antimicrobial susceptibility of urinary tract infection (uti) pathogens isolated in european hospitals. | 2001 | 11683792 |
| antibiotic susceptibility of bacterial strains isolated from patients with community-acquired urinary tract infections. | isolates from urine samples obtained during 1999 were identified and their susceptibility to antimicrobial agents studied along with any production of extended-spectrum beta-lactamases (esbl) by escherichia coli and klebsiella pneumoniae. a total of 13774 samples were analysed using an automatic system for the detection of bacterial atp (coral, usa). of these samples, 49% were reported to be positive and uncontaminated; bacteria most frequently isolated were e. coli (47%), proteus mirabilis (7%) ... | 2001 | 11673032 |
| zapa, a possible virulence factor from proteus mirabilis exhibits broad protease substrate specificity. | the opportunistic bacterium proteus mirabilis secretes a metalloprotease, zapa, considered to be one of its virulence factors due to its iga-degrading activity. however, the substrate specificity of this enzyme has not yet been fully characterized. in the present study we used fluorescent peptides derived from bioactive peptides and the oxidized beta-chain of insulin to determine the enzyme specificity. the bradykinin- and dynorphin-derived peptides were cleaved at the single bonds phe-ser and p ... | 2001 | 11668347 |
| identification of bacteria crucial to histamine accumulation in pacific mackerel during storage. | bacterial growth and histamine formation in pacific mackerel during storage at 0, 4, 15, and 25 degrees c were monitored. to identify bacterial species contributing to histamine formation, several groups of bacteria were isolated by using selective media under temperatures corresponding to the various storage conditions. initially, low counts of bacteria were found in the gill, skin, and intestine of fresh fish, and only weak histamine formers were found in the gill. histamine was found in the m ... | 2001 | 11601705 |
| [identification of vibrio spp bacteria on skin lesions of fisherman in the county of raposa-ma]. | the study was undertaken aiming at identifying bacteria from the county of raposa in the state of maranhão. the clinical sample was collected by using a swab and held in a cary-blair transport medium. enrichment in alkaline peptone water, isolation in tcbs selective indicator medium and biochemical coding of species were used for laboratory processing. fifty fisherman with age varying from 12-65 years took part on the study. vibrio bacteria isolated in 21 subjects had been identified. there was ... | 2001 | 11600905 |
| herpes simplex virus type 2 modulates the susceptibility of human bladder cells to uropathogenic bacteria. | the present study analyses the susceptibility of human bladder-derived cells (ht-1376) to the infection by herpes simplex virus type 2 (hsv-2) and chlamydia trachomatis, as well as to the adhesiveness of uropathogenic bacteria. ht-1376 cells were efficiently infected by hsv-2 strain 333, as demonstrated by immunofluorescence staining of viral antigens, titration of cytopathic effect, and visualisation by transmission electron microscopy. this cell model was also prone to c. trachomatis (serovar ... | 2001 | 11599790 |
| [massive pleural empyema caused by pyonephrosis]. | the pleural empyema has unusual origin at extralung causes. then, is essential to identify the primary process in order to obtain the clinical setting control. previously described, but even exceptional, this case report show an empyema in which it's origin was an asymtomatic pyonephrosis, caused because of a big kidney calculus and it's discovery was suspected by the bacillus results. the treatment must include appropriate antibiotic therapy and thoracic drainage, and also the drain of the puru ... | 2001 | 11594181 |
| proteus syndrome. | a case of proteus syndrome is presented, in which severe hemihypertrophy of the left trunk and left lower extremity, scoliosis, endometriosis and huge bizarre-shaped body tumors were observed. up to 22.6 kg of tumorous tissue was excised. this syndrome was first described in 1983. the name proteus comes from a greek mythical sea god who was able to change his body form freely. this syndrome has numerous features including hemihypertrophy, macrodactyly, various subcutaneous masses, scoliosis and ... | 2001 | 11586023 |
| isolation and structure elucidation of a new antifungal and antibacterial antibiotic produced by streptomyces sp. 201. | an antibacterial and antifungal antibiotic was isolated from the culture filtrate of streptomyces sp. 201, and its structure was determined as 2-methyl-heptyl isonicotinate by extensive use of nmr spectroscopy. the compound exhibited marked antimicrobial activity against bacillus subtilis, shigella sp., klebsiella sp., e. coli, proteus mirabilis, and the pathogenic fungi, fusarium moniliforme, f. semitectum, f. oxysporum, f. solani and rhizoctonia solani. | 2001 | 11577728 |
| enzymatic characterization of tem-63, a tem-type extended spectrum beta-lactamase expressed in three different genera of enterobacteriaceae from south africa. | the extended-spectrum beta-lactamase, tem-63, was identified in three separate genera of south african isolates: proteus mirabilis, klebsiella pneumoniae, and escherichia coli. this paper describes identification of the gene in these isolates and compares relative rates of hyrolysis between tem-63 and other known ceftazidimases. | 2001 | 11576794 |
| rheumatoid arthritis as an autoimmune disease caused by proteus urinary tract infections: a proposal for a therapeutic protocol. | 2001 | 11574986 | |
| rheumatoid arthritis: the future is not yet now. | 2001 | 11574984 | |
| [clinical significance of infection pathogen resistance in a urological clinic for selection of antibacterial therapy regimes for the treatment of complicated urinary infections]. | data on changes of etiological structure of the complicated urinary tract infections treated at the research institute of urology and hospital no. 47 at the 5-year period (1996-2000) are given. it was shown that the most important microorganisms were the following: enterobacter spp., e. coli, proteus spp., staphylococcus spp., enterococcus faecalis. the correlation between frequency of the microorganisms isolation and their susceptibility to antimicrobial agents at different periods was analysed ... | 2001 | 11573320 |
| [monitoring of uropathogens and their susceptibility to antibiotics]. | samples of urine collected from patients with complicated urology infection and hospitalized to the moscow region research clinical institute in 1986, 1991, 1995 and 1999 were analysed. of 11,444 samples examined, bacteriuria was estimated in 7143 samples. 9786 strains (29 genus) of bacteria were isolated--56.9 per cent as mono culture and 43.1 per cent as associations. susceptibility to 21 antibiotic was determined by disk diffusion method for 1607 strains; beta-lactamase production was determi ... | 2001 | 11573319 |
| bacterial swarming: a biochemical time-resolved ftir-atr study of proteus mirabilis swarm-cell differentiation. | fourier transform infrared spectroscopy was applied to the study of the differentiation process undergone by proteus mirabilis. this bacterium exhibits a remarkable dimorphism, allowing the cells to migrate on a solid substratum in a concerted manner yielding characteristic ring patterns. we performed an in situ noninvasive analysis of biochemical events occurring as vegetative cells differentiate into elongated, multinucleate, nonseptate, and hyperflagellated swarm cells. the major findings ari ... | 2001 | 11570895 |
| electrochemical biosensor array for the identification of microorganisms based on lectin-lipopolysaccharide recognition. | rapid identification of bacterial strains remains a well-known problem in applied medicine and, for viable pathogens, is an important diagnostic goal. we have investigated an electrochemical biosensor array, in which transduction is based on respiratory cycle activity measurements, where the microorganism's native respiratory chain is interrupted with non-native external oxidants. the selective biochemical recognition agents employed in this study are lectins that, once immobilized, recognize an ... | 2001 | 11569815 |
| [formation of intestinal microflora in children during the first year of their life]. | in 525 young children the state of intestinal microbiocenosis was studied every month of the first year their life. the study revealed that the process of the microflora formation lasted throughout the first year of their life and was characterized by dysbiotic disturbances. during this period the aggravation of dysbiotic changes in the intestine of these children on months 3, 6-7 and 11-12 was of particular importance. the formation of stable dysbacteriosis led to a decrease in the immunologica ... | 2001 | 11569262 |
| [clinical significance of microbiological monitoring of infective agents in an urological hospital in the selection of antibacterial therapy regimens]. | individual microbiological examination of each patient can improve significantly the response of urological patients to antibacterial therapy. 6000 cultures of opportunistic microorganisms isolated from patients with various infectious-inflammatory diseases were studied. most of them were gram-negative: p. aeruginosa, e. coli, enterobacter spp., proteus spp. of gram-positive most frequently occurred staphylococcus spp and enterococcus faecalis. the cultures showed different spectrum of the sensi ... | 2001 | 11569227 |
| gram-negative bacterial toe web infection: a survey of 123 cases from the district of cagliari, italy. | foot intertrigo is mostly caused by dermatophytes and yeasts, less frequently by gram-positive and gram-negative bacteria. nevertheless, the importance of polymicrobial infections and especially colonizations of pseudomonas aeruginosa can cause therapy problems in relation to antibiotic resistance and the risk of potentially lethal complications. | 2001 | 11568743 |
| scoliosis in proteus syndrome: case report. | the case of patient with scoliosis based on a rare hamartomatous, proteus syndrome, is reported. | 2001 | 11568716 |
| ligand diffusion in the catalase from proteus mirabilis: a molecular dynamics study. | the role of the channels and cavities present in the catalase from proteus mirabilis (pmc) was investigated using molecular dynamics (md) simulations. the reactant and products of the reaction, h(2)o(2) -->1/2 o(2) + h(2)o, catalyzed by the enzyme were allowed to diffuse to and from the active site. dynamic fluctuations in the structure are found necessary for the opening of the major channel, identified in the x-ray model, which allows access to the active site. this channel is the only pathway ... | 2001 | 11567083 |
| bioactive compounds from iostephane heterophylla (asteraceae). | the novel bisabolene sesquiterpenes 3-6, were isolated from iostephane heterophylla, using bioguided fractionation. the new compounds were determined to be (12r/12s)-12,13-epoxy-xanthorrhizols (3,4) and (12r/12s)-12,13-dihydro-12,13-dihydroxy-xanthorrizols (5,6) and their structures were characterized by analysis of spectroscopic data and by chemical correlation from xanthorrhizol (2). the stereochemistry at c-12 of 5 was deduced using the modified mosher experiment. some of the isolated compoun ... | 2001 | 11561451 |
| proteus syndrome: a case report. | proteus syndrome is a rare genetic disorder, characterized by partial gigantism of the hands and/or feet, asymmetry of the limbs, plantar hyperplasia, multiple hamartomatous subcutaneous tumors, hyperostoses, and long bone overgrowth. a one day old thai male infant is reported with macrosomia, hemihypertrophy of the left side of the face and left leg, large feet, macrodactyly of toes, plantar hyperplasia, large subcutaneous mass with a violet-red surface over the left side of the chest wall and ... | 2001 | 11560226 |
| isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. | the aim of this study was to determine the occurrence and role of yeasts, enteric gram-negative rods and enterococcus species in root-filled teeth with chronic apical periodontitis, and the antimicrobial effect of iodine potassium iodide (iki) irrigation. | 2001 | 11556508 |
| identification of the translocating bacteria in rats with acute liver injury and their relation to the bacterial flora of the intestinal mucosa. | the bacterial flora of the intestine and the bacteria found in liver, mesenteric lymph nodes, portal and arterial blood after d-galactosamine-induced liver injury, with and without pretreatment with lactobacillus plantarum dsm 9843, were studied in the rat. dominating representatives were identified to species level by 16s rdna sequencing and typed by randomly amplified polymorphic dna (rapd) and by restriction endonuclease analysis (rea) for strain definition. it was proven that bacterial strai ... | 2001 | 11552954 |
| [activity and thermal stability of acid phosphatase in homogenates of amoeba proteus, acclimated to various temperatures]. | activity and thermoresistance of acid phosphatase were determined in supernatant of amoeba proteus homogenates using 1-naphthyl phosphate (ph 4.0) and p-nitrophenyl phosphate (ph 5.5). although tartrate-resistant and tartrate-sensitive acid phosphatases hydrolyse both substrates, the former mainly hydrolyses p-nitrophenyl phosphate and the latter 1-naphthyl phosphate. a decrease in the activity of the total and tartrate-sensitive acid phosphatases, when using 1-naphthyl phosphate, and of the tot ... | 2001 | 11552654 |
| involvement of coenzyme a esters and two new enzymes, an enoyl-coa hydratase and a coa-transferase, in the hydration of crotonobetaine to l-carnitine by escherichia coli. | two proteins (caib and caid) were found to catalyze the reversible biotransformation of crotonobetaine to l-carnitine in escherichia coli in the presence of a cosubstrate (e.g., gamma-butyrobetainyl-coa or crotonobetainyl-coa). caib (45 kda) and caid (27 kda) were purified in two steps to electrophoretic homogeneity from overexpression strains. caib was identified as crotonobetainyl-coa:carnitine coa-transferase by maldi-tof mass spectrometry and enzymatic assays. the enzyme exhibits high cosubs ... | 2001 | 11551212 |
| [intestinal microbiocenosis in children with intestinal enzymopathy]. | 141 children with different kinds of intestinal enzymopathy were examined; of these, 33 had celiac disease, 39--the syndrome of celiac disease, 12--congenital lactase deficiency and 57--the syndrome of disaccharidase insufficiency. in these patients a significant decrease in the average characteristics of the main protective flora and the growth of hemolytic and lactose-negative enterobacteria were established. in all groups of patients increased amounts of proteus were detected, which was indic ... | 2001 | 11550576 |
| new aspects of the role of mr/p fimbriae in proteus mirabilis urinary tract infection. | proteus mirabilis, a common cause of urinary tract infection (uti), produces a number of different fimbriae including mannose-resistant proteus-like fimbriae (mr/p). the precise role of different p. mirabilis fimbriae in ascending uti has not yet been elucidated. in this study, a clinical isolate of p. mirabilis and an isogenic mutant unable to express mr/p were tested using different experimental approaches. they were tested for their ability to cause infection in an ascending co-infection mode ... | 2001 | 11549418 |
| [experimental study of photodynamic effect on bacterial wound microflora]. | the in vitro study of the influence of photodynamic action (with the use of photosensitizer "photosens", laser and non-laser irradiation) on the strains of the main representatives purulent wound microflora was carried out. staphylococcus aureus, s. epidermidis, pseudomonas aeruginosa, escherichia coli, proteus mirabilis cultures have been isolated from wound secretions and identified. the photosensitizer was shown to produce no bactericidal effect by itself. irradiation with laser and non-laser ... | 2001 | 11548270 |
| [humoral immune response in patients with bronchopulmanory infections to immunotherapy]. | the content of iga, igm, igg and the level of specific antibodies in the blood serum of patients with bronchopulmonary diseases after a course of immunotherapy with polycomponent vaccine b[symbol: see text]-4 was studied. a rise in the concentration of igm due to the synthesis of specific antibodies to proteus vulgaris, escherichia coli and klebsiella pneumoniae was found to occur. the examination of sick children revealed that a high proportion of them (54%) showed a pronounced decrease in the ... | 2001 | 11548259 |
| filamentous structures in amoeba proteus visualized in scanning microscope. | 2001 | 11544682 | |
| effects of blocking of endogenous rho family gtp-binding proteins on morphology, adhesion and locomotion of amoeba proteus. | 2001 | 11544664 | |
| identification and localisation of caldesmon-like protein in amoeba proteus. | 2001 | 11544656 | |
| intervening sequences in rrl genes and fragmentation of 23s rrna in genera of the family enterobacteriaceae. | intervening sequences (ivss) in the rrl genes for 23s rrna are transcribed but later removed by rnase iii without religation during rna processing, leading to fragmented rrna. we examined about 240 strains of the family enterobacteriaceae for presence of ivss using pcr. no ivss were detected in strains belonging to escherichia, shigella, enterobacter, erwinia, ewingella, hafnia, kluyvera, morganella, pantoea, or serratia. previously unreported ivss were detected in klebsiella oxytoca, citrobacte ... | 2001 | 11544246 |
| gravisensitivity of cells of several types. | researchers examined time-related changes in growth, motility, and some morphological-functional characteristics of cells and cell association in unicellular organisms, plants, and human and mice cell cultures. post-flight analysis included studies of cell formation, division rate, chemical composition, and ultrastructural organization of cells and their major organelles in weightlessness and hypergravity. unicellular organisms used in the studies were the ciliates tetrahymena pyriformis, dilept ... | 1998 | 11542335 |
| [number, activity and thermostability of the electrophoretic forms of acid phosphatase in amoeba proteus, cultured at different temperatures]. | in free-living amoebae (amoeba proteus, strain b), cultured at 10 and 25 degrees c, we compared the number, activity, and thermostability of separate electromorphs of triton-soluble acid phosphatase (acp) revealed by disc-electrophoresis in polyacrylamide gel using 2-naphthyl phosphate (ph 4.0) as a substrate. no differences in the number of acp electromorphs and their mobility were observed at both these temperatures. the total activity of acp electromorphas per unit of cellular protein and the ... | 2001 | 11534183 |
| bacteriology of diabetic foot. | to study the relative frequency of bacterial isolates cultured from diabetic foot infections and assess their comparative in vitro susceptibility to the commonly used antibacterial agents. | 2000 | 11533815 |
| cmy-2-producing salmonella enterica, klebsiella pneumoniae, klebsiella oxytoca, proteus mirabilis and escherichia coli strains isolated in spain (october 1999-december 2000). | cmy-2 plasmid-mediated ampc beta-lactamase (cmy-2) was detected in 21 isolates from two hospitals located in different geographical regions of spain between october 1999 and december 2000. the isolates comprised two salmonella enterica serovars (mikawasima and montevideo), 16 escherichia coli, one klebsiella pneumoniae, one klebsiella oxytoca and one proteus mirabilis. in addition to the expected resistance to beta-lactams, including extended-spectrum cephalosporins and cefoxitin, all isolates s ... | 2001 | 11533003 |
| repression of bacterial motility by a novel fimbrial gene product. | proteus mirabilis is a common uropathogen in patients with long-term catheterization or with structural or functional abnormalities in the urinary tract. the mannose-resistant, proteus-like (mr/p) fimbriae and flagellum are among virulence factors of p.mirabilis that contribute to its colonization in a murine model of ascending urinary tract infection. mrpj, the last of nine genes of the mrp operon, encodes a 107 amino acid protein that contains a putative helix-turn-helix domain. using transcri ... | 2001 | 11532949 |
| protean infectious types and frequent association with neurosurgical procedures in adult serratia marcescens cns infections: report of two cases and review of the literature. | serratia marcescens is a rare pathogen of adult central nervous system (cns) infection. we report on the clinical features and therapeutic outcomes of two adult patients with such infections. the clinical characteristics of 13 other reported adult cases are also included for analysis. the 15 cases were nine males and six females, aged 19-83 years, in whom, underlying post-neurosurgical states and ear operation were noted in 93% (14/15). fever and conscious disturbance were the most common clinic ... | 2001 | 11532558 |
| [proteus syndrome]. | 2001 | 11528880 | |
| site-directed mutagenesis and phylogenetic comparisons of the escherichia coli tus protein: dna-protein interactions alone can not account for tus activity. | the tus protein of escherichia coli is capable of arresting dna replication in an orientation-dependent manner when bound to specific sequences in the bacterial chromosome called ter sites. arrest of dna replication has been postulated to occur either by a barrier mechanism, where tus acts as a physical block to replication fork progression, or through protein-protein interactions between tus and some component of the replication fork. a previous mutational analysis of tus suggested that the ami ... | 2001 | 11523786 |
| bacterial modification of lps and resistance to antimicrobial peptides. | antimicrobial peptides (aps) are ubiquitous in nature and are thought to kill micro-organisms by affecting membrane integrity. these positively charged peptides interact with negative charges in the lps of gram-negative bacteria. a common mechanism of resistance to ap killing is lps modification. these modifications include fatty acid additions, phosphoethanolamine (petn) addition to the core and lipid a regions, 4-amino-4-deoxy-l-arabinose (ara4n) addition to the core and lipid a regions, acety ... | 2001 | 11521084 |
| comparative antimicrobial spectrum and activity of bms284756 (t-3811, a desfluoroquinolone) tested against 656 enterobacteriaceae, including preliminary in vitro susceptibility test comparisons and development. | bms284756 (t-3811), a novel des-f(6)-quinolone, was evaluated using isolates of enterobacteriaceae from the sentry antimicrobial surveillance program tested by etest (ab biodisk, solna, sweden), reference broth microdilution and disk diffusion (5-microg) methods. ciprofloxacin, levofloxacin, gemifloxacin and gatifloxacin were also tested by broth microdilution as comparator antimicrobial agents within the same drug class. the 656 isolate collection included species from the genera citrobacter, e ... | 2001 | 11516937 |
| prevalence of antimicrobial resistance among urinary tract pathogens isolated from female outpatients across the us in 1999. | in the united states, trimethoprim-sulphamethoxazole (tmp-smx) is the recommended first-line treatment for uncomplicated urinary tract infections (utis) in females, in regions with resistance rates of <10-20%. unfortunately, current data on regional resistance is often not readily available to physicians and regional variability in resistance remains largely unknown. this report presents antimicrobial susceptibility data for tmp-smx and three other commonly tested antimicrobials organized by sta ... | 2001 | 11516934 |
| urinary tract infections in patients with spinal cord lesions: treatment and prevention. | even though the mortality due to urinary tract complications has decreased dramatically during the last decades in individuals with spinal cord lesions (scl), urinary tract infections (uti) still cause significant morbidity in this population. complicated uti are caused by a much wider variety of organisms in individuals with scl than in the general population and are often polymicrobial. escherichia coli, pseudomonas spp., klebsiella spp., proteus spp., serratia spp., providencia spp., enteroco ... | 2001 | 11511022 |
| [comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1999). i. susceptibility distribution]. | the bacterial strains isolated from patients diagnosed as having urinary tract infections (utis) in 9 institutions in japan were supplied between the period of august 1999 to july 2000. then, the susceptibilities of them to many kinds of antimicrobial agents were investigated. the number of them were 499 strains. the breakdown of these strains was gram-positive bacteria as 31.3% and gram-negative bacteria as 68.7%. susceptibilities of these bacteria to antimicrobial agents were as follows; vanco ... | 2001 | 11510118 |
| epidemiology and frequency of resistance among pathogens causing urinary tract infections in 1,510 hospitalized patients: a report from the sentry antimicrobial surveillance program (north america). | bacterial urinary tract infections (utis) are an important cause of septicemia resulting in high mortality rates, prolonged hospital stays and increased healthcare costs. periodic reviews of pathogen frequency and susceptibility patterns impact on appropriate antimicrobial usage, leading to more effective prescribing practices. as part of the sentry antimicrobial surveillance program (sentry, 1998), participants collected 50 consecutive uti pathogens from patients hospitalized in 31 medical cent ... | 2001 | 11502382 |
| plant growth promotion and fungicidal activity in a siderophore-producing strain of proteus sp. | a proteus strain inhibited mycelial growth of fusarium oxysporum in vitro. seed bacterization showed significant plant growth promotion and fusarium-wilt suppression activity of phaseolus mungo in a gnotobiotic system. the culture filtrate of this strain exhibited three prominent bands in uv-vis spectra between 300 and 400 nm. the growth promotion assay of the extracted compound against different indicator organisms indicated the production of a compound related to a 2-oxoacid-type siderophore. ... | 2000 | 11501420 |
| diabetic foot: presentation and treatment. | the aim of this study is to determine the mode of presentation, medical and surgical treatment in diabetic foot. | 2000 | 11500678 |
| [determination of the antibacterial and antiviral activity of the essential oil from minthostachys verticillata (griseb.) epling]. | the in vitro antiviral activity of the essential oil from minthostachys verticillata was investigated against herpes simplex virus type 1 (hsv-1) and pseudorabies virus (prv). the viral inhibition was assayed employing viral plaque reduction assay. the antiviral activity of the essential oil specifically affects prv and hsv-1 multiplication, since it was found that non toxic effects on cells were observed at the concentrations assayed. the therapeutic index values were 10.0 and 9.5 for hsv-1 and ... | 2001 | 11494754 |
| covalent joining of the subunits of a homodimeric type ii restriction endonuclease: single-chain pvuii endonuclease. | the pvuii restriction endonuclease has been converted from its natural homodimeric form into a single polypeptide chain by tandemly linking the two subunits through a short peptide linker. the arrangement of the single-chain pvuii (sc pvuii) is (2-157)-glyserglygly-(2-157), where (2-157) represents the amino acid residues of the enzyme subunit and glyserglygly is the peptide linker. by introducing the corresponding tandem gene into escherichia coli, pvuii endonuclease activity could be detected ... | 2001 | 11491304 |
| bacteriuria in the elderly population in a developing country. | among 1470 elderly hospitalized and nonhospitalized people, 566 cases of bacteriuria were identified. there were 663 men (41.5% with bacteriuria) and 807 women (36.0% with bacteriuria). the overall prevalence of bacteriuria was 38.5%. more than 90% of the isolates were gram-negative organisms with proteus species being the predominant pathogen among men, with 68.1% seen among inpatients. escherichia coli was the main pathogen in women, with 62.0% recovered from inpatients. catheterization was se ... | 2001 | 11491272 |
| further studies on rpos in enterobacteria: identification of rpos in enterobacter cloacae and kluyvera cryocrescens. | rpos, the alternative sigma factor sigma(s), is important for bacterial survival under extreme conditions. many enterobacteria are opportunistic human pathogens and their ability to survive in a changing environment could be an essential step for their virulence. to determine the presence of this gene in enteric bacteria, an escherichia coli rpos probe was constructed and used to detect the presence of this gene in different species. a gene homologous to rpos was found in citrobacter amalonaticu ... | 2001 | 11491080 |
| structure of the o-specific polysaccharide of proteus mirabilis d52 and typing of this strain to proteus serogroup o33. | the acidic o-specific polysaccharide chain (o-antigen) of the lipopolysaccharide (lps) of proteus mirabilis strain d52 was studied using chemical analyses along with 1h-nmr and 13c-nmr spectroscopy, including 2d cosy, tocsy, roesy, h-detected 1h,13c and 1h,31p hmqc experiments. the polysaccharide was found to contain d-ribitol 5-phosphate (d-rib-ol-5-p) and ethanolamine phosphate (etn-p) and has the following structure: d-rib-ol-5-p (3) approximately 75% etnp(6)-->2)-beta-d-galp-(1-->3)-alpha-d- ... | 2001 | 11488930 |
| does drinking cranberry juice produce urine inhibitory to the development of crystalline, catheter-blocking proteus mirabilis biofilms? | to test the recommendation that to avoid the complications of long-term indwelling bladder catheterization (e.g. encrustation and blockage by crystalline proteus mirabilis biofilms) patients should drink cranberry juice. | 2001 | 11488728 |
| [clinico-laboratory parallels during celiac disease in children]. | forty-five patients with celiac disease and 73 with the celiakia syndrome were observed. the clinical picture was identical and was characterized by disordered intestinal absorption. depression of t and b lymphocytes was typical of both forms of the disease. high values of antigliadin iga and igg correlated with the severity of atrophic processes in the small intestinal mucosa. intestinal microflora was characterized by a decrease in the main defense flora and growth of hemolytical and lactonega ... | 2000 | 11486746 |
| dental unit waterlines: review and product evaluation. | dental practitioners must be knowledgeable regarding microbial contamination and biofilm formation in dental unit waterlines. education should stress the need for improvement in the quality of water delivered to patients during treatment. manufacturers must also play an important role by providing training and education regarding the proper use and maintenance of their systems. dental facilities, both public and private, need reliable methods to prevent the development of biofilms within duws. t ... | 2001 | 11484611 |
| antimicrobial activity of alstonia macrophylla: a folklore of bay islands. | the methanolic crude and methanol-aqueous extract of alstonia macrophylla leaves and n-butanol part of the crude extract showed antimicrobial activity against various strains of staphylococcus aureus, staphylococcus saprophyticus, streptococcus faecalis, escherichia coli, proteus mirabilis, trichophyton rubrum, trichophyton mentagrophytes var. mentagrophytes and microsporum gypseum. the minimum inhibitory concentration (mic) values ranges from 64 to 1000 microg/ml for bacteria and 32-128 mg/ml f ... | 2001 | 11483378 |
| coronal leakage following three obturation techniques. | to compare coronal bacterial and india ink leakage in three different obturation techniques with the smear layer having been removed. | 2001 | 11482141 |
| etiologies of the urinary tract infections in a yemeni city. | to determine the causative agents of urinary tract infection in yemeni patients in sana'a city, and to determine the antibiotic susceptibilities of these agents in vitro. | 2001 | 11479641 |
| association of germline mutation in the pten tumour suppressor gene and proteus and proteus-like syndromes. | the molecular aetiology of proteus syndrome (ps) remains elusive. germline mutations in pten cause cowden syndrome and bannayan-riley-ruvalcaba syndrome, which are hereditary hamartoma syndromes. some features-eg, macrocephaly, lipomatosis, and vascular malformations-can be seen in all three syndromes. we examined pten in patients with ps and undefined proteus-like syndromes (ps-like) and identified de-novo germline mutations in two of nine patients with ps and three of five patients with ps-lik ... | 2001 | 11476841 |
| pten mutations are uncommon in proteus syndrome. | 2001 | 11476065 | |
| blood stream infections in a medical intensive care unit: spectrum and antibiotic susceptibility pattern. | to determine the type and sensitivity pattern of causative organisms of septicaemia in intensive care unit, to prepare a guideline for empirical antibiotic therapy. | 2001 | 11475775 |
| [black nails caused by proteus mirabilis]. | black nails caused by proteus mirabilis were seen in a motor mechanic and in a petrol pump attendant. proteus mirabilis is a gram-negative bacillus that generates hydrogen sulfide. this compound reacts with traces of metals in the nail plate such as zinc, nickel, cobalt, iron, manganese, tin, copper and lead. metal sulfides blacken the nail plate. the protracted course of the discoloration over months corresponds to the slow reactions of metals with hydrogen sulfide. the disappearance of the bla ... | 2001 | 11475651 |
| advances in disinfection testing and modelling. | to develop a set of kinetic equations which more ably describe the disinfection process. | 2001 | 11473601 |
| the formulation of an effective topical antibacterial product containing ocimum gratissimum leaf essential oil. | the antibacterial potential of ocimum gratissimum essential oil was explored. liquid and semisolid formulations of the oil were designed in a variety of bases for topical antiseptic medication. the products were evaluated by agar diffusion assay against type strains and clinical isolates from boil, wound and pimples. remarkable antibacterial effects, higher than those of commercial antiseptic products, were demonstrated at 2% ocimum oil concentration in some bases. the properties of base into wh ... | 2001 | 11472827 |
| asymmetry: molecular, biologic, embryopathic, and clinical perspectives. | this overview of asymmetry addresses the following topics: chiral molecules; asymmetric signaling molecules, including n-cadherin, shh, fgf8, lefty1, lefty2, nodal, pitx2, activin betab, activin receptor iia, and csnr; situs abnormalities; asymmetric cell division; laterality in humans and animals; behavioral asymmetry in humans and animals; asymmetric embryopathies, including tessier-type "clefts"; hemiasymmetries such as hemihyperplasia, hemihypoplasia, and hemiatrophy; asymmetric vascular syn ... | 2001 | 11471152 |
| the effect of ship scrapping industry and its associated wastes on the biomass production and biodiversity of biota in in situ condition at alang. | the main pollutants for the ship scrapping industry and its associated wastes at alang are heavy metals, petroleum hydrocarbon and bacterial contaminations. the concentration of iron, manganese, cobalt, copper, zinc, lead, cadmium, nickel and mercury were 25 to 15,500% more at nearshore station of alang as compared to control site at piram. the concentration of heavy metals in the nearshore station of alang was always higher than its concentration at 10 km away. the concentration of petroleum hy ... | 2001 | 11468924 |
| interrelations of organism prevalence, specimen collection method, and host age, sex, and breed among 8,354 canine urinary tract infections (1969-1995). | selected information was compiled from canine urinalyses and urine cultures conducted between january 1969 and december 1995. eight thousand three hundred fifty-four microbial isolates (bacteria and fungi) included 4,873 isolates from females and 3,481 from males. ten bacterial genera accounted for 96.3% of the urinary isolates, including escherichia coli (44.1%), staphylococcus spp. (11.6%), proteus spp. (9.3%), klebsiella spp. (9.1%), enterococcus spp. (8.0%), and streptococcus spp. (5.4%) as ... | 2001 | 11467591 |
| the effects of pentoxifylline treatment on bacterial translocation after hemorrhagic shock in rats. | bacterial translocation is thought to be responsible for infectious complications after hemorrhagic shock. the aim of this study was to investigate the effects of pentoxifylline treatment on bacterial translocation in animals subjected to hemorrhagic shock. thirty-one wistar albino rats (280-360 g) were divided into three groups: sham (n=10), shock (n=11), and shock-pentoxifylline (n=10). blood was not withdrawn from sham rats. shock rats were subjected to 30 min of shock followed by reinfusion ... | 2001 | 11467403 |
| new antioxidant and antimicrobial ellagic acid derivatives from pteleopsis hylodendron. | bioassay-guided isolation of two new compounds, 3,4-methylenedioxy-3'-o-methyl-4'-o-glucoside ellagic acid (1) and the pteleoellagic acid derivative (2), from the stem bark of pteleopsis hylodendron is reported along with 3,4-methylenedioxy-3'-o-methyl ellagic acid (3), 3,3'-di-o-methyl ellagic acid (4) and 3,3',4'-tri-o-methyl ellagic acid (5), which were obtained for the first time from this plant. the structures of these compounds were elucidated with the help of spectroscopic studies. compou ... | 2001 | 11458450 |
| the generation of superoxide anion in blood platelets in response to different forms of proteus mirabilis lipopolysaccharide: effects of staurosporin, wortmannin, and indomethacin. | lipopolysaccharide (lps), the major component of the outer membrane of gram-negative bacteria may activate blood platelets. the aim of our study was to evaluate the effects of different forms of proteus mirabilis lps and isolated lipid a and polysaccharide part on the production of superoxide radicals in blood platelets and to estimate the role staurosporin, wortmannin and indomethacin on this process. we compared the generation of superoxide radicals in platelets treated with lps after preincub ... | 2001 | 11457473 |