Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| myd88 signaling promotes both mucosal homeostatic and fibrotic responses during salmonella-induced colitis. | salmonella enterica serovar typhimurium is a clinically important gram-negative, enteric bacterial pathogen that activates several toll-like receptors (tlrs). while tlr signaling through the adaptor protein myd88 has been shown to promote inflammation and host defense against the systemic spread of s. typhimurium, curiously, its role in the host response against s. typhimurium within the mammalian gastrointestinal (gi) tract is less clear. we therefore used the recently described salmonella-indu ... | 2012 | 22679002 |
| a positive selection approach identifies residues important for folding of salmonella enterica pat, an n(ε)-lysine acetyltransferase that regulates central metabolism enzymes. | in salmonella enterica, the protein acetyltransferase (pat) enzyme is part of the sirtuin-dependent acylation/deacylation system (sdpads) that modulates the activity of several proteins via the acylation of lysine residues critical to their activities. pat is a ~98 kda protein with two distinct domains, an n-terminal acyl-coa synthetase (ndp-forming) domain (~700 aa) and a c-terminal acetyltransferase domain (~160 aa), with homology to proteins of the gcn5-related n-acetyltransferase (gnat) supe ... | 2012 | 22677774 |
| impact of hfq on the intrinsic drug resistance of salmonella enterica serovar typhimurium. | salmonella enterica is an important enteric pathogen, and its various serovars cause both systemic and intestinal diseases in humans and domestic animals. the emergence of multidrug-resistant strains of salmonella, leading to increased morbidity and mortality, has further complicated its management. hfq is an rna chaperon that mediates the binding of small rnas to mrna and assists in post-transcriptional gene regulation in bacteria. although hfq is related to important phenotypes including virul ... | 2012 | 22675323 |
| emergence of salmonella enterica serovar infantis harboring inci1 plasmid with bla(ctx-m-14) in a broiler farm in japan. | cefotaxime (ctx)-resistant and -susceptible salmonella enterica serovar infantis isolates obtained from broilers raised on a farm in january 2010 in japan were characterized to establish their resistance determinants. the ctx-resistant isolates produced ctx-m-14 extended-spectrum β-lactamase and harbored 2 distinct plasmid of approximately 140- and 95-kb, whereas the ctx-susceptible isolates harbored one 140-kb plasmid. the 95-kb plasmids were replicon typed as inci1 carrying the bla(ctx-m-14) g ... | 2012 | 22673563 |
| characterization of salmonella enterica serovar enteritidis isolates recovered from blood and stool specimens in thailand. | bacteremia due to salmonella spp. is a life-threatening condition and is commonly associated with immune compromise. a 2009 observational study estimated risk factors for the ten most common non-typhoidal salmonella (nts) serovars isolated from thai patients between 2002-2007. in this study, 60.8% of salmonella enterica serovar enteritidis isolates (n = 1517) were recovered from blood specimens and infection with salmonella serovar enteritidis was a statistically significant risk factor for bact ... | 2012 | 22672324 |
| surface-immobilization of chromatographically purified bacteriophages for the optimized capture of bacteria. | bacteriophages offer interesting alternatives to antibodies for the specific capture and detection of pathogenic bacteria onto biosensing surfaces. procedures for the optimal chemical immobilization of lytic bacteriophages onto surfaces are presented. more specifically, the removal of lysate contaminants from bacteriophage suspensions by size exclusion chromatography significantly increases the resultant planar surface density of immobilized bacteriophages. e. coli t4 and salmonella enterica ser ... | 2012 | 22666653 |
| antibiotic resistance of salmonella enterica serovar typhi in kolkata, india, and in vitro experiments on effect of combined chemotherapy. | this communication states the changing patterns of salmonella enterica serovar typhi (s. typhi) isolates causing enteric fever in and around kolkata, india. among the isolates resistance to ampicillin (a), chloramphenicol (c), cotrimoxazole (co) and tetracycline (t) were plasmid mediated; the plasmid was unstable in s. typhi, and the other enteric bacteria like escherichia coli, klebsiella pneumoniae and proteus vulgaris were found to be the potential source of dissemination of such plasmids int ... | 2012 | 22666124 |
| the mammalian microrna response to bacterial infections. | micrornas are small rnas that post-transcriptionally regulate eukaryotic gene expression. in addition to their involvement in a wide range of physiological and pathological processes, including viral infections, micrornas are increasingly implicated in the eukaryotic response to bacterial pathogens. recent studies have characterized changes in host microrna expression following infection with exclusively extracellular (helicobacter pylori) or intracellular (salmonella enterica) gram-negative bac ... | 2012 | 22664920 |
| variable carbon catabolism among salmonella enterica serovar typhi isolates. | salmonella enterica serovar typhi (s. typhi) is strictly a human intracellular pathogen. it causes acute systemic (typhoid fever) and chronic infections that result in long-term asymptomatic human carriage. s. typhi displays diverse disease manifestations in human infection and exhibits high clonality. the principal factors underlying the unique lifestyle of s. typhi in its human host during acute and chronic infections remain largely unknown and are therefore the main objective of this study. | 2012 | 22662115 |
| srfj, a salmonella type iii secretion system effector regulated by phop, rcsb, and iolr. | virulence-related type iii secretion systems are present in many gram-negative bacterial pathogens. these complex devices translocate proteins, called effectors, from the bacterium into the eukaryotic host cell. here, we identify the product of srfj, a salmonella enterica serovar typhimurium gene regulated by ssrb, as a new substrate of the type iii secretion system encoded by salmonella pathogenicity island 2. the n-terminal 20-amino-acid segment of srfj was recognized as a functional secretion ... | 2012 | 22661691 |
| quantitative proteomic analysis of type iii secretome of enteropathogenic escherichia coli reveals an expanded effector repertoire for attaching/effacing bacterial pathogens. | type iii secretion systems are central to the pathogenesis and virulence of many important gram-negative bacterial pathogens, and elucidation of the secretion mechanism and identification of the secreted substrates are critical to our understanding of their pathogenic mechanisms and developing potential therapeutics. stable isotope labeling with amino acids in cell culture-based mass spectrometry is a quantitative and highly sensitive proteomics tool that we have previously used to successfully ... | 2012 | 22661456 |
| a constitutively mannose-sensitive agglutinating salmonella enterica subsp. enterica serovar typhimurium strain, carrying a transposon in the fimbrial usher gene stbc, exhibits multidrug resistance and flagellated phenotypes. | static broth culture favors salmonella enterica subsp. enterica serovar typhimurium to produce type 1 fimbriae, while solid agar inhibits its expression. a transposon inserted in stbc, which would encode an usher for stb fimbriae of a non-flagellar salmonella enterica subsp. enterica serovar typhimurium lb5010 strain, conferred it to agglutinate yeast cells on both cultures. rt-pcr revealed that the expression of the fimbrial subunit gene fima, and fimz, a regulatory gene of fima, were both incr ... | 2012 | 22654583 |
| screening of microbial contamination and antimicrobial activity of sea cucumber holothuria polii. | microbiological studies were carried out on microbial contamination and antimicrobial activity of sea cucumber holothuria polii collected from mediterranean sea at abu-kir shore of alexandria, egypt. the obtained results revealed the presence of isolates of five human gram-negative pathogenic bacteria, representing five genera were identified to species level, including, esherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella sp. and shigella sp. in addition, an yeast candida ... | 2013 | 22653870 |
| a novel capture compound for the identification and analysis of cyclic di-gmp binding proteins. | the second messenger cyclic di-gmp is a near-ubiquitous signaling molecule that globally alters bacterial cell physiology to promote biofilm formation and community behavior. much progress was made in recent years towards the identification and characterization of diguanylate cyclases and phosphodiersterases, enzymes involved in the synthesis and degradation of this signaling compound. in contrast, our knowledge of the nature and mechanistic details of c-di-gmp effector proteins lags behind, pri ... | 2012 | 22652488 |
| molecular typing and resistance analysis of travel-associated salmonella enterica serotype typhi. | salmonella enterica serotype typhi is a human pathogen causing 12 to 30% mortality and requiring antibiotic therapy to control the severity of the infection. typhoid fever in united states is often associated with foreign travel to areas of endemicity. increasing resistance to multiple drugs, including quinolones, is associated with decreased susceptibility to ciprofloxacin (dcs). we investigated 31 clinical strains isolated in florida from 2007 to 2010, associated with travel to six countries, ... | 2012 | 22649021 |
| selective purification of recombinant neuroactive peptides using the flagellar type iii secretion system. | the structure, assembly, and function of the bacterial flagellum involves about 60 different proteins, many of which are selectively secreted via a specific type iii secretion system (t3ss) (j. frye et al., j. bacteriol. 188:2233-2243, 2006). the t3ss is reported to secrete proteins at rates of up to 10,000 amino acid residues per second. in this work, we showed that the flagellar t3ss of salmonella enterica serovar typhimurium could be manipulated to export recombinant nonflagellar proteins thr ... | 2012 | 22647788 |
| enhanced influenza virus-like particle vaccines containing the extracellular domain of matrix protein 2 and a toll-like receptor ligand. | the extracellular domain of matrix protein 2 (m2e) is conserved among influenza a viruses. the goal of this project is to develop enhanced influenza vaccines with broad protective efficacy using the m2e antigen. we designed a membrane-anchored fusion protein by replacing the hyperimmunogenic region of salmonella enterica serovar typhimurium flagellin (flic) with four repeats of m2e (4.m2e-tflic) and fusing it to a membrane anchor from influenza virus hemagglutinin (ha). the fusion protein was in ... | 2012 | 22647270 |
| antimicrobial food packaging film based on the release of lae from evoh. | the aim of this work was to develop antimicrobial films for active packaging applications containing the natural antimicrobial compound lae (lauramide arginine ethyl ester) in evoh copolymers with different mol % ethylene contents (i.e. evoh-29 and evoh-44). evoh-29 and evoh-44 films were made by casting and incorporating 0.25%, 1%, 5%, and 10% lae in the film forming solution (w/w with respect to polymer weight). previously, the minimum inhibitory concentration (mic) and the minimum bactericida ... | 2012 | 22640726 |
| structural characterization of the plig lysozyme inhibitor family. | several gram-negative bacteria protect themselves against the lytic action of host lysozymes by producing specific proteinaceous inhibitors. so far, four different families of lysozyme inhibitors have been identified including ivy (inhibitor of vertebrate lysozyme), mlic/plic (membrane associated/periplasmic inhibitor of c-type lysozyme), plii and plig (periplasmic inhibitors of i- and g-type lysozymes, respectively). here we provide the first crystallographic description of the plig family. cry ... | 2012 | 22634186 |
| development of antimicrobial films for microbiological control of packaged salad. | the aim of the present work was to characterize the antimicrobial efficiency of films consisting of pp/evoh structures with oregano essential oil and citral. both substances are known for their antimicrobial activity based on their interaction with the cell membrane. the films developed were used to pack minimally processed salads, combining modified atmosphere technology to extend shelf-life and active packaging technology to reduce possible microbiological risks. the antimicrobial activity of ... | 2012 | 22633535 |
| community-acquired bacterial bloodstream infections in developing countries in south and southeast asia: a systematic review. | information about community-acquired bacteraemia in developing countries in south and southeast asia is scarce. we aimed to establish the case fraction of bacteraemia in febrile patients admitted to hospital. we searched four databases and identified studies of south and southeast asia published between 1990 and 2010 that prospectively assessed patients admitted to hospital and from whom a blood culture was taken. we reviewed 17 eligible studies describing 40,644 patients. pathogenic organisms w ... | 2012 | 22632186 |
| effects of indole on drug resistance and virulence of salmonella enterica serovar typhimurium revealed by genome-wide analyses. | many gram-positive and gram-negative bacteria produce large quantities of indole as an intercellular signal in microbial communities. indole demonstrated to affect gene expression in escherichia coli as an intra-species signaling molecule. in contrast to e. coli, salmonella does not produce indole because it does not harbor tnaa, which encodes the enzyme responsible for tryptophan metabolism. our previous study demonstrated that e. coli-conditioned medium and indole induce expression of the acra ... | 2012 | 22632036 |
| genome sequences of five salmonella enterica serovar heidelberg isolates associated with a 2011 multistate outbreak in the united states. | salmonella enterica serovar heidelberg has caused numerous outbreaks in humans. here, we report draft genomes of five isolates of serovar heidelberg associated with the recent (2011) multistate outbreak linked to ground turkey in the united states. isolates 2011k-1110 and 2011k-1132 were recovered from humans, while isolates 2011k-1138, 2011k-1224, and 2011k-1225 were recovered from ground turkey. whole-genome sequence analysis of these isolates provides a tool for studying the short-term evolut ... | 2012 | 22628505 |
| antibiotic susceptibility pattern and the indicator of decreased ciprofloxacin susceptibility of salmonella enterica serovar typhi isolated from dhulikhel hospital, nepal. | monitoring the antibiotic susceptibility pattern of salmonella enterica serovar typhi (s. typhi) is important for efficiently managing cases of typhoid fever. in this study, the antimicrobial susceptibility patterns of 114 s. typhi isolates, which were collected from a university hospital in nepal during july 2009-december 2010, were investigated by disc diffusion assays. all of the s. typhi isolates were sensitive to amoxycillin-clavulanic acid. more than 95% of the isolates were sensitive to c ... | 2012 | 22627312 |
| antibiotic resistant salmonella and vibrio associated with farmed litopenaeus vannamei. | salmonella and vibrio species were isolated and identified from litopenaeus vannamei cultured in shrimp farms. shrimp samples showed occurrence of 3.3% of salmonella and 48.3% of vibrio. the isolates were also screened for antibiotic resistance to oxolinic acid, sulphonamides, tetracycline, sulfamethoxazole/trimethoprim, norfloxacin, ampicillin, doxycycline hydrochloride, erythromycin, chloramphenicol, and nitrofurantoin. salmonella enterica serovar corvallis isolated from shrimp showed individu ... | 2012 | 22619583 |
| salmonella strains isolated from galápagos iguanas show spatial structuring of serovar and genomic diversity. | it is thought that dispersal limitation primarily structures host-associated bacterial populations because host distributions inherently limit transmission opportunities. however, enteric bacteria may disperse great distances during food-borne outbreaks. it is unclear if such rapid long-distance dispersal events happen regularly in natural systems or if these events represent an anthropogenic exception. we characterized salmonella enterica isolates from the feces of free-living galápagos land an ... | 2012 | 22615968 |
| characterization of modular bacteriophage endolysins from myoviridae phages obp, 201φ2-1 and pvp-se1. | peptidoglycan lytic enzymes (endolysins) induce bacterial host cell lysis in the late phase of the lytic bacteriophage replication cycle. endolysins obpgp279 (from pseudomonas fluorescens phage obp), pvp-se1gp146 (salmonella enterica serovar enteritidis phage pvp-se1) and 201φ2-1gp229 (pseudomonas chlororaphis phage 201φ2-1) all possess a modular structure with an n-terminal cell wall binding domain and a c-terminal catalytic domain, a unique property for endolysins with a gram-negative backgrou ... | 2012 | 22615864 |
| impact of antibiotic mic on infection outcome in patients with susceptible gram-negative bacteria: a systematic review and meta-analysis. | the objective of this study was to analyze the impact of mic values within the susceptible range of antibiotics on the outcomes of patients with gram-negative infections. the pubmed and scopus electronic databases were searched. we identified 13 articles (1,469 patients) that studied the impact of antibiotic mics on the outcomes of infections; β-lactams were studied in 10 of them. infections due to salmonella enterica strains with high fluoroquinolone mics were associated with more treatment fai ... | 2012 | 22615292 |
| role of mouse peptidoglycan recognition protein pglyrp2 in the innate immune response to salmonella enterica serovar typhimurium infection in vivo. | peptidoglycan recognition proteins (pgrps) are a family of innate pattern recognition molecules that bind bacterial peptidoglycan. while the role of pgrps in drosophila innate immunity has been extensively studied, how the four mammalian pgrp proteins (pglyrp1 to pglyrp4) contribute to host defense against bacterial pathogens in vivo remains poorly understood. pglyrp1, pglyrp3, and pglyrp4 are directly bactericidal in vitro, whereas pglyrp2 is an n-acetylmuramyl-l-alanine amidase that cleaves pe ... | 2012 | 22615249 |
| intestinal epithelial cells modulate antigen-presenting cell responses to bacterial dna. | intestinal epithelial cells and antigen-presenting cells orchestrate mucosal innate immunity. this study investigated the role of bacterial dna in modulating epithelial and bone marrow-derived antigen-presenting cells (bm-apcs) and subsequent t-lymphocyte responses. murine mode-k epithelial cells and bm-apcs were treated with dna from either bifidobacterium breve or salmonella enterica serovar dublin directly and under coculture conditions with cd4(+) t cells. apical stimulation of mode-k cells ... | 2012 | 22615241 |
| a maximum likelihood qtl analysis reveals common genome regions controlling resistance to salmonella colonization and carrier-state. | the serovars enteritidis and typhimurium of the gram-negative bacterium salmonella enterica are significant causes of human food poisoning. fowl carrying these bacteria often show no clinical disease, with detection only established post-mortem. increased resistance to the carrier state in commercial poultry could be a way to improve food safety by reducing the spread of these bacteria in poultry flocks. previous studies identified qtls for both resistance to carrier state and resistance to salm ... | 2012 | 22613937 |
| plasmid-mediated quinolone resistance determinant qnrb19 in non-typhoidal salmonella enterica strains isolated in venezuela. | 2012 | 22610715 | |
| a retrospective study of community-acquired salmonella infections in patients attending public hospitals in lagos, nigeria. | a retrospective cohort study on salmonella-associated diseases (sads) was conducted in 14 public hospitals across lagos state, nigeria, between 1999 and 2008. | 2012 | 22610704 |
| the acyl homoserine lactone receptor, sdia, of escherichia coli and salmonella enterica serovar typhimurium does not respond to indole. | in this study, we tested the hypothesis that the sdia proteins of escherichia coli and salmonella enterica serovar typhimurium respond to indole. while indole was found to have effects on gene expression and biofilm formation, these effects were not sdia dependent. however, high concentrations of indole did inhibit n-acyl-l-homoserine lactone (ahl) sensing by sdia. we conclude that sdia does not respond to indole but indole can inhibit sdia activity in e. coli and salmonella. | 2012 | 22610437 |
| identification of a third osmoprotectant transport system, the osmu system, in salmonella enterica. | the growth of salmonella enterica serovar typhimurium mutants lacking the prop and prou osmoprotectant transport systems is stimulated by glycine betaine in high-osmolarity media, suggesting that this organism has an additional osmoprotectant transport system. bioinformatic analysis revealed that the genome of this organism contains a hitherto-unidentified operon, designated osmu, consisting of four genes whose products show high similarity to abc-type transport systems for osmoprotectants in ot ... | 2012 | 22609924 |
| the evaluation of a pcr-based method for identification of salmonella enterica serotypes from environmental samples and various food matrices. | the most commonly used method for serotyping salmonella spp. is based on the kaufmann-white scheme, and is composed of serological reactions using antibodies to lps agglutinins. the multiplex pcr used in this investigation was established by kim et al. to serotype the 30 most common clinical salmonella serotypes, as determined by cdc. the pcr assay consists of two five-plex reactions and a single two-plex pcr reaction, based on six genetic loci from salmonella enterica serotype typhimurium and f ... | 2012 | 22608224 |
| influence of serotype on the growth kinetics and the ability to form biofilms of salmonella isolates from poultry. | the influence of the serotype on the growth behaviour and the ability to form biofilms of salmonella enterica strains was investigated. the relationships between biofilm formation and growth kinetic parameters were also determined. a total of 69 strains (61 isolates from poultry and 8 reference strains from culture collections) belonging to 10 serotypes (s. enterica serotype typhimurium, s. newport, s. paratyphi b, s. poona, s. derby, s. infantis, s. enteritidis, s. virchow, s. agona and s. typh ... | 2012 | 22608221 |
| salmonella cross-contamination in swine abattoirs in portugal: carcasses, meat and meat handlers. | in this study the occurrence of salmonella in swine, pork meat and meat handlers along with their clonal relatedness is evaluated at abattoir level. samples from the lymph nodes, carcass surface and meat of 100 pigs and 45 meat handlers were collected in eight abattoirs (july 2007-august 2008). salmonella isolates were serotyped and genotyped by pulsed-field gel electrophoresis (pfge). from the pigs tested, 42 produced at least one positive sample. a relatively high frequency of salmonella occur ... | 2012 | 22607810 |
| first outbreak of food poisoning caused by salmonella enterica subspecies enterica serovar berta in italy. | to provide an epidemiologic interpretation of a suspected outbreak of food poisoning caused by salmonella enterica subspecies enterica serovar berta strains isolated from humans and from the leftovers of the implicated foods (cream, dairy-based desserts and eggs). | 2012 | 22607387 |
| stm2209-stm2208 (opvab): a phase variation locus of salmonella enterica involved in control of o-antigen chain length. | stm2209 and stm2208 are contiguous loci annotated as putative protein-coding genes in the chromosome of salmonella enterica. lack of homologs in related enterobacteria and low g+c content suggest that s. enterica may have acquired stm2209-stm2208 by horizontal transfer. stm2209 and stm2208 are co-transcribed from a promoter upstream stm2209, and their products are inner (cytoplasmic) membrane proteins. analysis with the bacterial adenylate cyclase two-hybrid system suggests that stm2209 and stm2 ... | 2012 | 22606300 |
| a gold nanoparticles based immuno-bioprobe for detection of vi capsular polysaccharide of salmonella enterica serovar typhi. | a rapid and sensitive gold-nanobioprobe based immunoassay format has been presented for the detection of capsular vi polysaccharide of salmonella enterica serovar typhi (surface antigen) using anti-vi antibodies. the vi antigen was extracted from serovar typhi cells, under the optimised growth conditions for its over-expression. anti-vi antibodies were produced and conjugated with gold nanoparticles (gnps) of definite size (~30 nm), which served as the nano-bioprobe in the detection system. a sa ... | 2012 | 22592180 |
| shedding light on salmonella carriers. | host-to-host transmission in most salmonella serovars occurs primarily via the fecal-oral route. salmonella enterica serovar typhi is a human host-adapted pathogen and some s. typhi patients become asymptomatic carriers. these individuals excrete large numbers of the bacteria in their feces and transmit the pathogen by contaminating water or food sources. the carrier state has also been described in livestock animals and is responsible for food-borne epidemics. identification and treatment of ca ... | 2012 | 22591832 |
| invasive non-typhoidal salmonella disease: an emerging and neglected tropical disease in africa. | invasive strains of non-typhoidal salmonellae have emerged as a prominent cause of bloodstream infection in african adults and children, with an associated case fatality of 20-25%. the clinical presentation of invasive non-typhoidal salmonella disease in africa is diverse: fever, hepatosplenomegaly, and respiratory symptoms are common, and features of enterocolitis are often absent. the most important risk factors are hiv infection in adults, and malaria, hiv, and malnutrition in children. a dis ... | 2012 | 22587967 |
| caenorhabditis elegans immune conditioning with the probiotic bacterium lactobacillus acidophilus strain ncfm enhances gram-positive immune responses. | although the immune response of caenorhabditis elegans to microbial infections is well established, very little is known about the effects of health-promoting probiotic bacteria on evolutionarily conserved c. elegans host responses. we found that the probiotic gram-positive bacterium lactobacillus acidophilus ncfm is not harmful to c. elegans and that l. acidophilus ncfm is unable to colonize the c. elegans intestine. conditioning with l. acidophilus ncfm significantly decreased the burden of a ... | 2012 | 22585961 |
| dietary rice bran promotes resistance to salmonella enterica serovar typhimurium colonization in mice. | dietary rice bran consists of many bioactive components with disease fighting properties; including the capacity to modulate the gut microbiota. studies point to the important roles of the gut microbiota and the mucosal epithelium in the establishment of protection against enteric pathogens, such as salmonella. the ability of rice bran to reduce the susceptibility of mice to a salmonella infection has not been previously investigated. therefore, we hypothesized that the incorporation of rice bra ... | 2012 | 22583915 |
| application of the random forest method to analyse epidemiological and phenotypic characteristics of salmonella 4,[5],12:i:- and salmonella typhimurium strains. | salmonella enterica 4,[5],12:i:- is a monophasic variant of s. typhimurium. in the last decade, its prevalence rose sharply. although s. 4,[5],12:i:- and s. typhimurium are known to pose a considerable public health risk, there is no detailed information on the circulation of these serovars in italy, particularly as far as veterinary isolates are concerned. for this reason, a data set of 877 strains isolated in the north-east of italy from foodstuffs, animals and environment was analysed during ... | 2012 | 22583909 |
| a modified weibull model for growth and survival of listeria innocua and salmonella typhimurium in chicken breasts during refrigerated and frozen storage. | the potential of food-borne pathogens to survive and grow during refrigerated and frozen storage has raised serious concerns over the safety of stored poultry products. in this study, the effect of refrigeration and freezing temperatures (-20, -12, 0, 4, and 8°c) on growth and survival of listeria innocua and salmonella enterica serovar typhimurium in raw chicken breasts for storage times of 3, 7, 10, 14, and 21 d were investigated. a modified weibull model was also developed to analyze the micr ... | 2012 | 22582310 |
| salmonella biofilm development depends on the phosphorylation status of rcsb. | the rcs phosphorelay pathway is a complex signaling pathway involved in the regulation of many cell surface structures in enteric bacteria. in response to environmental stimuli, the sensor histidine kinase (rcsc) autophosphorylates and then transfers the phosphate through intermediary steps to the response regulator (rcsb), which, once phosphorylated, regulates gene expression. here, we show that salmonella biofilm development depends on the phosphorylation status of rcsb. thus, unphosphorylated ... | 2012 | 22582278 |
| instability of escherichia coli r-factors in salmonella enterica serovar typhi involves formation of recombinant composite plasmid structures. | in spite of a well-documented ability of samonella enterica typhi strains to receive r factors from escherichia coli and other enterobacteria, epidemiological data show that typhi is a rather poor host of antibiotic-resistance genes and in fact, of plasmids, suggesting that most of the plasmids naturally acquired by typhi strains become unstable and eventually segregate. we have previously reported evidence that each of three plasmids conjugatively transferred to s. enterica typhi experienced de ... | 2012 | 22579995 |
| comparative proteomic analysis of salmonella tolerance to the biocide active agent triclosan. | concern has been expressed about the overuse of biocides in farm animal production and food industries. biocide application can create selective pressures that lead to increased tolerance to one or more of these compounds and are concomitant with the emergence of cross-resistance to antibiotics. a triclosan sensitive salmonella enterica serovar typhimurium and the isogenic triclosan tolerant mutant were studied at the proteomic level in order to elucidate cellular mechanisms that facilitate bioc ... | 2012 | 22579747 |
| structures of a unique o-polysaccharide of edwardsiella tarda pcm 1153 containing an amide of galacturonic acid with 2-aminopropane-1,3-diol and an abequose-containing o-polysaccharide shared by e. tarda pcm 1145, pcm 1151 and pcm 1158. | lipopolysaccharides of four strains of edwardsiella tarda were degraded by mild acid hydrolysis, and the released o-polysaccharides were isolated by gpc and studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h, (1)h cosy, tocsy, roesy, (1)h, (13)c hmbc, hsqc and hsqc-tocsy experiments. the o-polysaccharide from e. tarda pcm 1153 was found to contain d-gala, d-glcnac, d-gal and 2-amino-1,3-propanediol (gron). in the tetrasaccharide repeating unit ... | 2012 | 22578768 |
| c1 inhibitor suppresses the endotoxic activity of a wide range of lipopolysaccharides and interacts with live gram-negative bacteria. | human c1 inhibitor (c1inh) prevents endotoxin shock via a direct interaction with gram-negative bacterial lipopolysaccharide (lps) and improves survival in animal models of sepsis. in this report, we further characterize the interaction of c1inh with lps and whole live bacteria. we investigate c1inh interactions with lps from five different strains of gram-negative enteric bacteria known to participate in the pathogenesis of human sepsis. treatment with c1inh improved survival in mice with endot ... | 2012 | 22576004 |
| selection of salmonella enterica serovar typhi genes involved during interaction with human macrophages by screening of a transposon mutant library. | the human-adapted salmonella enterica serovar typhi (s. typhi) causes a systemic infection known as typhoid fever. this disease relies on the ability of the bacterium to survive within macrophages. in order to identify genes involved during interaction with macrophages, a pool of approximately 10(5) transposon mutants of s. typhi was subjected to three serial passages of 24 hours through human macrophages. mutants recovered from infected macrophages (output) were compared to the initial pool (in ... | 2012 | 22574205 |
| salmonella enterica serotype enteritidis: increasing incidence of domestically acquired infections. | salmonella enterica causes an estimated 1 million cases of domestically acquired foodborne illness in humans annually in the united states; enteritidis (se) is the most common serotype. public health authorities, regulatory agencies, food producers, and food processors need accurate information about rates and changes in se infection to implement and evaluate evidence-based control policies and practices. | 2012 | 22572674 |
| chronic proliferative rhinitis associated with salmonella enterica subspecies diarizonae serovar 61:k:1, 5, (7) in sheep in spain. | a chronic proliferative rhinitis in sheep associated with salmonella enterica subspecies diarizonae serovar 61:k:1, 5, (7) is described. ten adult sheep from eight traditionally managed rasa aragonesa flocks located in aragon (spain) were studied. clinical signs began with bilateral thick mucus nasal discharge, wheezing and snoring and progressed to partial or complete obstruction of the nostrils. necropsy examination revealed swollen ventral nasal turbinates with a roughened mucosal surface par ... | 2012 | 22572396 |
| prevalence of human pathogens and indicators in stormwater runoff in brisbane, australia. | elevated numbers of enteric pathogens in the receiving waters following a storm event can be a serious public health concern. the purpose of this study was to conduct a preliminary investigation into the presence of human pathogens of concern in urban stormwater runoff. the involvement of a human sewage as a potential source of contamination was also investigated by using microbial source tracking methods. water samples (20 l) were collected after storm events and during the dry weather from six ... | 2012 | 22572123 |
| salmonella enterica serovar typhi plasmid impairs dendritic cell responses to infection. | salmonella enterica serovar typhi (s. typhi) evades from innate immunity by expression of a variety of pathogenic factors. the "pr(st98)" plasmid of s. typhi is involved in multidrug-resistant and virulence of s. typhi. however, its exact effect on host cell function remains elusive. dendritic cells (dcs) play an important role in shaping immune response against salmonella. for the purpose of investigation whether pr(st98) might target dcs involved in adaptive immune response, murine dcs were in ... | 2012 | 22569891 |
| frequency and antimicrobial resistance of salmonella serotypes on beef carcasses at small abattoirs in jalisco state, mexico. | the prevalence and antimicrobial resistance of salmonella serotypes on beef carcasses from four small abattoirs in jalisco state, mexico, were investigated during a 10-month period. following u.s. department of agriculture food safety and inspection service protocols, salmonella was isolated from 78 (15.4%) beef carcasses (n = 505) after the final carcass water wash. isolation frequency differed by establishment (p < 0.05) and was higher (p < 0.05) during the wet season (may through september) f ... | 2012 | 22564935 |
| effect of farm type on within-herd salmonella prevalence, serovar distribution, and antimicrobial resistance. | salmonella represents a major challenge to the pig industry, as pork presents a risk for human salmonellosis. in this study, we have examined the effect of farm type on the prevalence of fattening pigs shedding salmonella on 12 farms at risk for harboring salmonella. on six open (grow-to-finish) and six closed (farrow-to-finish) farms, the prevalence of pigs shedding salmonella was determined on two occasions approximately 2 months apart. the serovar, phage type, and antimicrobial resistance of ... | 2012 | 22564934 |
| microbiological analysis of bovine lymph nodes for the detection of salmonella enterica. | bovine peripheral lymph nodes (lns) have been identified as a potential source of salmonella when trim containing these nodes is incorporated into ground beef. studies examining the prevalence of salmonella in peripheral lns of cattle are few in number, and the microbiological methods used for these analyses have not been validated. given that salmonella contamination may be found on postintervention carcasses, it is important to understand the extent to which salmonella contamination from surro ... | 2012 | 22564933 |
| salmonella enterica: a surprisingly well-adapted intracellular lifestyle. | the infectious intracellular lifestyle of salmonella enterica relies on the adaptation to nutritional conditions within the salmonella-containing vacuole (scv) in host cells. we summarize latest results on metabolic requirements for salmonella during infection. this includes intracellular phenotypes of mutant strains based on metabolic modeling and experimental tests, isotopolog profiling using (13)c-compounds in intracellular salmonella, and complementation of metabolic defects for attenuated m ... | 2012 | 22563326 |
| effects of x-ray treatments on pathogenic bacteria, inherent microflora, color, and firmness on whole cantaloupe. | inactivation of inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on whole cantaloupes using x-ray at different doses (0.1, 0.5, 1.0, 1.5, and 2.0 kgy) was studied. the effect of x-ray on quality parameters (color and texture) of untreated and treated whole cantaloupes was instrumentally determined. the effect of x-ray on microflora counts (mesophilic counts, psychrotrophic counts and yeast and mold counts) of untreated and treated whole canta ... | 2012 | 22560020 |
| vulnerabilities in yersinia pestis caf operon are unveiled by a salmonella vector. | during infection, yersinia pestis uses its f1 capsule to enhance survival and cause virulence to mammalian host. since f1 is produced in large quantities and secreted into the host tissues, it also serves as a major immune target. to hold this detrimental effect under proper control, y. pestis expresses the caf operon (encoding the f1 capsule) in a temperature-dependent manner. however, additional properties of the caf operon limit its expression. by overexpressing the caf operon in wild-type sa ... | 2012 | 22558420 |
| polyamines are required for virulence in salmonella enterica serovar typhimurium. | sensing and responding to environmental cues is a fundamental characteristic of bacterial physiology and virulence. here we identify polyamines as novel environmental signals essential for virulence of salmonella enterica serovar typhimurium, a major intracellular pathogen and a model organism for studying typhoid fever. central to its virulence are two major virulence loci salmonella pathogenicity island 1 and 2 (spi1 and spi2). spi1 promotes invasion of epithelial cells, whereas spi2 enables s ... | 2012 | 22558361 |
| virulence of 32 salmonella strains in mice. | virulence and persistence in the balb/c mouse gut was tested for 32 strains of salmonella enterica for which genome sequencing is complete or underway, including 17 serovars within subspecies i (enterica), and two representatives of each of the other five subspecies. only serovar paratyphi c strain baa1715 and serovar typhimurium strain 14028 were fully virulent in mice. three divergent atypical enteritidis strains were not virulent in balb/c, but two efficiently persisted. most of the other str ... | 2012 | 22558320 |
| functional and computational analysis of amino acid patterns predictive of type iii secretion system substrates in pseudomonas syringae. | bacterial type iii secretion systems (t3sss) deliver proteins called effectors into eukaryotic cells. although n-terminal amino acid sequences are required for translocation, the mechanism of substrate recognition by the t3ss is unknown. almost all actively deployed t3ss substrates in the plant pathogen pseudomonas syringae pathovar tomato strain dc3000 possess characteristic patterns, including (i) greater than 10% serine within the first 50 amino acids, (ii) an aliphatic residue or proline at ... | 2012 | 22558318 |
| prevalence and characterization of motile salmonella in commercial layer poultry farms in bangladesh. | salmonella is a globally widespread food-borne pathogen having major impact on public health. all motile serovars of salmonella enterica of poultry origin are zoonotic, and contaminated meat and raw eggs are an important source to human infections. information on the prevalence of salmonella at farm/holding level, and the zoonotic serovars circulating in layer poultry in the south and south-east asian countries including bangladesh, where small-scale commercial farms are predominant, is limited. ... | 2012 | 22558269 |
| targeted therapy via oral administration of attenuated salmonella expression plasmid-vectored stat3-shrna cures orthotopically transplanted mouse hcc. | the development of rna interference-based cancer gene therapies has been delayed due to the lack of effective tumor-targeting delivery systems. attenuated salmonella enterica serovar typhimurium (s. typhimurium) has a natural tropism for solid tumors. we report here the use of attenuated s. typhimurium as a vector to deliver shrna directly into tumor cells. constitutively activated signal transducer and activator of transcription 3 (stat3) is a key transcription factor involved in both hepatocel ... | 2012 | 22555509 |
| signature tagged mutagenesis in the functional genetic analysis of gastrointestinal pathogens. | signature tagged mutagenesis is a genetic approach that was developed to identify novel bacterial virulence factors. it is a negative selection method in which unique identification tags allow analysis of pools of mutants in mixed populations. the approach is particularly well suited to functional genetic analysis of the gastrointestinal phase of infection in foodborne pathogens and has the capacity to guide the development of novel vaccines and therapeutics. in this review we outline the techni ... | 2012 | 22555467 |
| complete nucleotide sequence of pvqs1 containing a quinolone resistance determinant from salmonella enterica serovar virchow associated with foreign travel. | nalidixic acid-resistant salmonella enterica serovars kentucky (n = 5) and virchow (n = 6) cultured from individuals were investigated for the presence of plasmid-mediated quinolone resistance (pmqr) determinants. | 2012 | 22553140 |
| dynamics of salmonella infection of macrophages at the single cell level. | salmonella enterica causes a range of diseases. salmonellae are intracellular parasites of macrophages, and the control of bacteria within these cells is critical to surviving an infection. the dynamics of the bacteria invading, surviving, proliferating in and killing macrophages are central to disease pathogenesis. fundamentally important parameters, however, such as the cellular infection rate, have not previously been calculated. we used two independent approaches to calculate the macrophage ... | 2012 | 22552918 |
| co-administration of live attenuated salmonella enterica serovar typhimurium expressing swine interleukin-18 and interferon-α provides enhanced th1-biased protective immunity against inactivated vaccine of pseudorabies virus. | the co-administration of two or more cytokines may generate additive or synergistic effects for controlling infectious diseases. however, the practical use of cytokine combinations for the modulation of immune responses against inactivated vaccine has not been demonstrated in livestock yet, primarily due to protein stability, production, and costs associated with mass administration. in light of the current situation, we evaluated the immunomodulatory functions of the combined administration of ... | 2012 | 22551253 |
| sanitation of selected ready-to-eat intermediate-moisture foods of animal origin by e-beam irradiation. | to optimize the sanitation treatment of ready-to-eat (rte) intermediate-moisture foods (imf), the behavior of listeria monocytogenes scott a (cip 103575), l. innocua (ntc 11288), salmonella enterica serovar typhimurium (cect 443), and escherichia coli o157:h7 (cect 4972) following treatment with electron-beam irradiation has been studied. as food matrixes, three rte vacuum-packed products (iberian dry-cured ham, dry beef [cecina], and smoked tuna) were used. although an irradiation treatment is ... | 2012 | 22551071 |
| characterization of salmonella enterica and detection of the virulence genes specific to diarrheagenic escherichia coli from poultry carcasses in ouagadougou, burkina faso. | one hundred chicken carcasses purchased from three markets selling poultry in ouagadougou, burkina faso, between june 2010 and october 2010 were examined for their microbiological quality. the presence of salmonella was investigated using standard bacteriological procedures, and the isolates obtained were serotyped and tested for antimicrobial susceptibility. the presence of virulence-associated genes of the five main pathogroups of diarrheagenic escherichia coli-shiga toxin-producing e. coli (s ... | 2012 | 22551070 |
| srnas and the virulence of salmonella enterica serovar typhimurium. | the combination of genomics and high-throughput cdna sequencing technologies has facilitated the identification of many small rnas (srnas) that play a central role in the post-transcriptional gene regulation of salmonella enterica serovar typhimurium. to date, most of the functionally characterized srnas have been involved in the regulation of processes which are not directly linked to virulence. just five srnas have been found to affect the ability of salmonella to replicate within mammalian ce ... | 2012 | 22546935 |
| glutathione deficiency in type 2 diabetes impairs cytokine responses and control of intracellular bacteria. | individuals with type 2 diabetes are at increased risk of acquiring melioidosis, a disease caused by burkholderia pseudomallei infection. although up to half of melioidosis patients have underlying diabetes, the mechanisms involved in this increased susceptibility are unknown. we found that b. pseudomallei-infected pbmcs from diabetic patients were impaired in il-12p70 production, which resulted in decreased ifn-γ induction and poor bacterial killing. the defect was specific to the il-12-ifn-γ a ... | 2012 | 22546856 |
| hypochlorous acid and hydrogen peroxide-induced negative regulation of salmonella enterica serovar typhimurium ompw by the response regulator arca. | hydrogen peroxide (h2o2) and hypochlorous acid (hocl) are reactive oxygen species that are part of the oxidative burst encountered by salmonella enterica serovar typhimurium (s. typhimurium) upon internalization by phagocytic cells. in order to survive, bacteria must sense these signals and modulate gene expression. growing evidence indicates that the arcab two component system plays a role in the resistance to reactive oxygen species. we investigated the influx of h2o2 and hocl through ompw and ... | 2012 | 22545862 |
| identification of genes responsible for biofilm formation or virulence in salmonella enterica serovar pullorum. | salmonella living in biofilms are more resistant to chemical and physical stresses. however, information regarding the regulation of genes involved in biofilm formation for salmonella enterica serovar pullorum remains limited. in this study, eight mutants with knockout of genes ompr, rpos, rfag, rfbh, rhle, mete, spia, or steb from the salmonella enterica serovar pullorum strain s6702 were constructed. phenotypic analysis revealed that all mutants were similar to the wild-type strain in growth r ... | 2012 | 22545539 |
| comparison of the prevalence of salmonella infection in layer hens from commercial layer farms with high and low rodent densities. | a comparison on the prevalence of salmonella infection in layer hens from commercial layer farms with high and low rodent densities was investigated. out of 280 laying hens sampled from three commercial layer farms with high rodent densities, salmonella enterica subsp. enterica serovar enteritidis (salmonella enteritidis) was isolated from 20 (7.14%) hens and salmonella enterica subsp. enterica serovar infantis (salmonella infantis) from three (1.07%) hens. in contrast, layer hens sampled from f ... | 2012 | 22545525 |
| heterogeneity of persistence of salmonella enterica serotype senftenberg strains could explain the emergence of this serotype in poultry flocks. | salmonella enterica serotype senftenberg (s. senftenberg) has recently become more frequent in poultry flocks. moreover some strains have been implicated in severe clinical cases. to explain the causes of this emergence in farm animals, 134 s. senftenberg isolates from hatcheries, poultry farms and human clinical cases were analyzed. persistent and non-persistent strains were identified in chicks. the non-persistent strains disappeared from ceca a few weeks post inoculation. this lack of persist ... | 2012 | 22545136 |
| no beneficial effects evident for enterococcus faecium ncimb 10415 in weaned pigs infected with salmonella enterica serovar typhimurium dt104. | salmonella enterica serovar typhimurium dt 104 is the major pathogen for salmonellosis outbreaks in europe. we tested if the probiotic bacterium enterococcus faecium ncimb 10415 can prevent or alleviate salmonellosis. therefore, piglets of the german landrace breed that were treated with e. faecium (n = 16) as a feed additive and untreated controls (n = 16) were challenged with s. typhimurium 10 days after weaning. the presence of salmonellae in feces and selected organs, as well as the immune r ... | 2012 | 22544257 |
| evidence for lack of acquisition of tolerance in salmonella enterica serovar typhimurium atcc 14028 after exposure to subinhibitory amounts of origanum vulgare l. essential oil and carvacrol. | overnight exposure of salmonella enterica serovar typhimurium to sublethal amounts of origanum vulgare essential oil (ov) and carvacrol (car) did not result in direct and cross-bacterial protection. cells subcultured with increasing amounts of ov or car survived up to the mic of either compound, revealing few significant changes in bacterial susceptibility. | 2012 | 22544235 |
| in vitro inhibition of expression of virulence genes responsible for colonization and systemic spread of enteric pathogens using bifidobacterium bifidum secreted molecules. | enteric pathogens such as salmonella enterica serovar typhimurium and enterohaemorrhagic escherichia coli require an initial indispensable step of attachment or invasion of enterocytes before they can produce systemic disease and translocate to their target organs. prevention of either of these steps will result in an avirulent state and limit their pathogenicity. in vitro tests demonstrated that molecules secreted by bifidobacterium bifidum interfere with both attachment and invasion. the main ... | 2012 | 22541391 |
| the transcriptional landscape and small rnas of salmonella enterica serovar typhimurium. | more than 50 y of research have provided great insight into the physiology, metabolism, and molecular biology of salmonella enterica serovar typhimurium (s. typhimurium), but important gaps in our knowledge remain. it is clear that a precise choreography of gene expression is required for salmonella infection, but basic genetic information such as the global locations of transcription start sites (tsss) has been lacking. we combined three rna-sequencing techniques and two sequencing platforms to ... | 2012 | 22538806 |
| conversion of viable but nonculturable enteric bacteria to culturable by co-culture with eukaryotic cells. | viable but nonculturable (vbnc) vibrio cholerae non-o1/non-o139, v. parahaemolyticus, enterohemorrhagic escherichia coli, enterotoxigenic e. coli, enteropathogenic e. coli, shigella flexneri, and salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., ht-29, caco-2, t84, hela, intestine 407, and cho cells. | 2012 | 22537150 |
| high pressure inactivation of salmonella on jalapeño and serrano peppers destined for direct consumption or as ingredients in mexican salsa and guacamole. | in summer of 2008, the united states witnessed one of the largest multi-state salmonellosis outbreak linked to the consumption of jalapeño and serrano peppers tainted with salmonella enterica serovar saintpaul. the first objective of this study was to assess the application of high hydrostatic pressure (hhp) to decontaminate jalapeño and serrano peppers from this pathogen. jalapeño and serrano peppers were inoculated with a five-strain cocktail of salmonella to a final level of ca. ~6 log cfu/g ... | 2012 | 22534353 |
| functional dissection of ssef, a membrane-integral effector protein of intracellular salmonella enterica. | during intracellular life, the bacterial pathogen salmonella enterica translocates a complex cocktail of effector proteins by means of the spi2-encoded type iii secretions system. the effectors jointly modify the endosomal system and vesicular transport in host cells. ssef and sseg are two effectors encoded by genes within salmonella pathogenicity island 2 and both effector associate with endosomal membranes and microtubules and are involved in the formation of salmonella-induced filaments. our ... | 2012 | 22529968 |
| development of an antigen-capture monoclonal antibody-based enzyme-linked immunosorbent assay and comparison with culture for detection of salmonella enterica serovar enteritidis in poultry hatchery environmental samples. | an antigen-capture enzyme-linked immunosorbent assay (elisa) was developed for use as a presumptive screening test for detection of salmonella enterica serovar enteritidis and other group d salmonella in poultry hatchery environments. a mixture of 2 monoclonal antibodies that recognize different forms of the lipopolysaccharide o-antigen was used for specific detection of group d salmonella. the performance of the elisa was evaluated in comparison to standard salmonella culture procedures. cultur ... | 2012 | 22529117 |
| variability of rna quality extracted from biofilms of foodborne pathogens using different kits impacts mrna quantification by qpcr. | the biofilm formation by foodborne pathogens is known to increase the problem related with surface disinfection procedure in the food processing environment and consequent transmission of these pathogens into the population. messenger rna has been increasingly used to understand the action and the consequences of disinfectants in the virulence on such biofilms. rna quality is an important requirement for any rna-based analysis since the quality can impair the mrna quantification. therefore, we e ... | 2012 | 22526569 |
| storing drinking-water in copper pots kills contaminating diarrhoeagenic bacteria. | microbially-unsafe water is still a major concern in most developing countries. although many water-purification methods exist, these are expensive and beyond the reach of many people, especially in rural areas. ayurveda recommends the use of copper for storing drinking-water. therefore, the objective of this study was to evaluate the effect of copper pot on microbially-contaminated drinking-water. the antibacterial effect of copper pot against important diarrhoeagenic bacteria, including vibrio ... | 2012 | 22524115 |
| requirement of siderophore biosynthesis for plant colonization by salmonella enterica. | contaminated fresh produce has become the number one vector of nontyphoidal salmonellosis to humans. however, salmonella enterica genes essential for the life cycle of the organism outside the mammalian host are for the most part unknown. screening deletion mutants led to the discovery that an aroa mutant had a significant root colonization defect due to a failure to replicate. aroa is part of the chorismic acid biosynthesis pathway, a central metabolic node involved in aromatic amino acid and s ... | 2012 | 22522683 |
| antioxidant and antibacterial activities of the leaf essential oil and its constituents furanodienone and curzerenone from lindera pulcherrima (nees.) benth. ex hook. f. | lindera pulcherrima (nees.) benth. ex hook. f. (family: lauraceae), an evergreen shrub, is an important medicinal plant distributed in temperate himalayan regions. the leaves and bark are used as spice in cold, fever, and cough. | 2012 | 22518079 |
| distribution of salmonella serovars and antimicrobial susceptibility of salmonella enteritidis from poultry in zimbabwe. | a study was carried out to determine the distribution and antimicrobial susceptibility profiles of salmonella serovars from chickens from large-scale commercial (lsc), small-scale commercial (ssc), and rural free-range (rfr) farms of zimbabwe. pooled cloacal swabs were collected for culture and isolation of salmonella spp. a chi-square test was used to assess distribution differences of salmonellas among the farming sectors. approximately 10% (283/2833) of the swabs were positive for salmonella ... | 2012 | 22515540 |
| the extradomain a of fibronectin enhances the efficacy of lipopolysaccharide defective salmonella bacterins as vaccines in mice. | the extradomain a from fibronectin (eda) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. here, we investigated the adjuvant effect of eda in mice immunizations against salmonella enterica subspecies enterica serovar enteritidis (salmonella enteritidis). since lipopolysaccharide (lps) is a major virulence factor and the lps o-polysaccharide (o-ps) is the immunodominant antigen in serological diagno ... | 2012 | 22515195 |
| [highly ciprofloxacin resistant salmonella enterica serovar kentucky isolates in turkey meat and a human patient]. | in recent years in france, england, wales, denmark and the usa about 500 human infections occurred, which were caused by multidrug-resistant salmonella enterica serovar (s.) kentucky isolates displaying high-level resistance to fluoroquinolones (ciprofloxacin, mic > or = 4 mg/l). the responsible clone was referred to as st198-x1.to determine whether this clone is also present in german s. kentucky isolates, the national reference laboratory for salmonella (nrl-salm) at the bfr analyzed the trend ... | 2013 | 22515025 |
| detection and characterization of pct-like plasmid vectors for blactx-m-14 in escherichia coli isolates from humans, turkeys and cattle in england and wales. | to detect and characterize escherichia coli strains and pct-like plasmids implicated in the dissemination of the ctx-m-14 gene in animals and humans, in england and wales. | 2012 | 22514265 |
| early immune dynamics following infection with salmonella enterica serovars enteritidis, infantis, pullorum and gallinarum: cytokine and chemokine gene expression profile and cellular changes of chicken cecal tonsils. | salmonella enterica subspecies enterica infection remains a serious problem in a wide range of animals and in man. poultry-derived food is the main source of human infection with the non-host-adapted serovars while fowl typhoid and pullorum disease are important diseases of poultry. we have assessed cecal colonization and immune responses of newly hatched and older chickens to salmonella serotypes enteritidis, infantis, gallinarum and pullorum. s. enteritidis and s. infantis colonized the ceca m ... | 2012 | 22512820 |
| improved immune responses against avian influenza virus following oral vaccination of chickens with ha dna vaccine using attenuated salmonella typhimurium as carrier. | this study evaluates the immune responses of single avian influenza virus (aiv) ha dna vaccine immunization using attenuated salmonella enterica sv. typhimurium as an oral vaccine carrier and intramuscular (im) dna injection. one-day-old specific-pathogen-free (spf) chicks immunized once by oral gavage with 10(9) salmonella colony-forming units containing plasmid expression vector encoding the ha gene of a/ck/malaysia/5858/04 (h5n1) (pcdna3.1.h5) did not show any clinical manifestations. serum h ... | 2012 | 22512819 |
| high incidence of campylobacter concisus in gastroenteritis in north jutland, denmark: a population-based study. | the incidence of non-thermophilic campylobacter species was assessed in an unselected population-based study in a mixed urban and rural community in north jutland, denmark. in a 2-year study period, 11,314 faecal samples from 8302 patients with gastroenteritis were cultured with supplement of the filter method. we recovered a high incidence of campylobacter concisus (annual incidence 35/100,000 inhabitants), almost as high as the common campylobacter jejuni/coli. in contrast, there was a very lo ... | 2013 | 22512739 |
| a case of urinary tract infection due to salmonella enterica serovar virchow and review of the related literature. | nontyphoid salmonella (nts) serotypes can cause gastroenteritis, bacteriemia, and focal infections. however, these focal infections, including urinary tract infections (uti), are occasionally observed; in particular, the presence of several predisposing factors, such as immunodeficiency and structural abnormality in the urinary tract, increase the possibility of the occurrence of infection. we present a case of uti caused by salmonella enterica serovar virchow in an elderly and debilitated patie ... | 2012 | 22510290 |