Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| the structure of the pineal complex in a common indian teleost, catla catla: evidence for pineal-induced inhibition of testicular function within an annual reproductive cycle. | the structure of the pineal complex and the annual reproductive cycle in a major indian carp, catla catla, were investigated in the present study. additionally, given the well-known inhibitory effects of the pineal on reproductive function in mammals, attempts were made to investigate whether or not the pineal exerts an inhibitory influence on reproductive function in this piscine species as well. sexually adult animals were utilized in all experiments. the cytomorphology of the pineal complex a ... | 2003 | 12856801 |
| group ii metabotropic glutamate receptors regulate the vulnerability to hypoxic brain damage. | we examined the expression of metabotropic glutamate (mglu) receptors in species of fish that differ for their vulnerability to anoxic brain damage. although expression of mglu1a and mglu5 receptors was similar in the brain of all species examined, expression of mglu2/3 receptors was substantially higher in the brain of anoxia-tolerant species (i.e., the carp carassius carassius and the goldfish carassius auratus) than in the brain of species that are highly vulnerable to anoxic damage, such as ... | 2003 | 12853420 |
| evaluating the radiation of the pomc gene in teleosts: characterization of american eel pomc. | a distinctive feature of the pituitary hormone precursor, proopiomelanocortin (pomc), is the presence of multiple melanocortin core sequences (hfrw), and one copy of the opioid, beta-endorphin. in the older lineages of ray-finned fish (i.e., orders acipenseriformes and semionotiformes), certain extant lobe-finned fish (australian lungfish and african lungfish), and the tetrapods there are three melanocortin regions in pomc: acth/alphamsh, beta-msh, and gamma-msh. however, among the teleosts, the ... | 2003 | 12849961 |
| stability and emulsion-forming ability of water-soluble fish myofibrillar protein prepared by conjugation with alginate oligosaccharide. | carp myofibrillar protein (mf) was conjugated with alginate oligosaccharide (ao) through the maillard reaction under low relative humidity, and the functional properties of the mf-ao conjugate were investigated under different nacl concentrations and ph levels. mf became highly solubilized at lower nacl concentrations by conjugation with ao, with a slight loss of available lysine. the thermal stability of mf was effectively improved by conjugation with ao. heat treatment at 80 degrees c for 2 h ... | 2003 | 12848513 |
| gadolinium, a mechano-sensitive channel blocker, inhibits osmosis-initiated motility of sea- and freshwater fish sperm, but does not affect human or ascidian sperm motility. | exposure to hypo-osmotic or hyperosmotic environment triggers the initiation of fish sperm motility. in this article, we report that calcium and potassium channel blockers do not influence motility of puffer fish sperm but calmodulin antagonists reversibly decrease it, suggesting that calmodulin-ca(2+) interactions are prerequisite for the initiation of sperm motility in this species. gadolinium (a stretch activated ion channel blocker) decreased the motility of puffer fish sperm from 92 +/- 3% ... | 2003 | 12845597 |
| myocardial revascularization before carotid endarterectomy. | diffuse atherosclerosis involving more than 1 vascular bed is a challenging problem. the natural histories of carotid and coronary atherosclerosis are clearly intertwined. the optimal strategies for treatment of patients who present with carotid artery stenoses and co-existent coronary artery disease (cad) remain controversial. minimally invasive screening tests for cad are often unreliable, and patients presenting with significant extracranial carotid artery stenoses should usually be assumed t ... | 2003 | 12832990 |
| clonal diversity in the japanese silver crucian carp, carassius langsdorfii inferred from genetic markers. | the japanese silver crucian carp, carassius langsdorfii, is a naturally polyploid fish that reproduces by gynogenesis, resulting in a geographically clustered clonal population. to determine the amount of clonal diversity in this species, we used genetic markers to compare fish from several japanese locations. samples were collected from lake kasumigaura (ibaraki pref.), lake imba (chiba pref.), lake fukushimagata (niigata pref.), lake biwa (shiga pref.), lake koyama (tottori pref.), and the niy ... | 2003 | 12832833 |
| recent duplication of the common carp (cyprinus carpio l.) genome as revealed by analyses of microsatellite loci. | genome duplications may have played a role in the early stages of vertebrate evolution, near the time of divergence of the lamprey lineage. additional genome duplication, specifically in ray-finned fish, may have occurred before the divergence of the teleosts. the common carp (cyprinus carpio) has been considered tetraploid because of its chromosome number (2n = 100) and its high dna content. we studied variation using 59 microsatellite primer pairs to better understand the ploidy level of the c ... | 2003 | 12832638 |
| the effect of high dose of cortisol on glucose-6-phosphatase and fructose-1,6-bisphosphatase activity, and glucose and fructose-2,6-bisphosphate concentration in carp tissues (cyprinus carpio l.). | the effect of a high dose of cortisol (200 mg kg(-1) body mass) on juvenile carp was investigated. the activity of glucose-6-phosphatase in liver and of fructose-1,6-bisphosphatase in liver, kidney and muscle, the serum glucose and fructose-2,6-bisphosphate concentration as well as the serum concentration of the injected hormone were measured after 24, 72 and 216 h after intraperitoneal cortisol injection. the activities of fructose-1,6-bisphosphatase in liver and kidney and glucose-6-phosphatas ... | 2003 | 12831768 |
| study of estrogenic effects on carp (cyprinus carpio) exposed to sewage treatment plant effluents. | to investigate whether male carp (cyprinus carpio) are really feminized by estrogen-like substances in effluent from sewage treatment plants (stps) in japan, exposure tests were performed using carp in water tanks that were receiving effluent from a stp. during eight weeks of exposure, we had measured their serum vitellogenin (vtg), a precursor of yolk protein specific for females that is synthesized in their liver by estrogen-like substances and that is, therefore, a biomarker of feminization. ... | 2003 | 12830946 |
| peculiar gene organisation and incomplete splicing of sea bass (dicentrarchus labrax l) interleukin-1beta. | in this paper we describe the gene organisation of sea bass dicentrarchus labrax interleukin-1beta (il-1beta) and the presence of incompletely spliced forms of this gene. interestingly, the sea bass il-1beta gene comprises five exons and four introns, thus being remarkably different from other known teleost and mammalian genes. the sizes of the introns were typically much shorter than in mammals and this feature, together with the loss of two introns, gave a much smaller gene than in mammals (2. ... | 2003 | 12823998 |
| effects of fish introduction on the length of the tail of cryptomonads in mesocosm experiments. | two different morphs of the small cryptomonad, plagioselmis prolonga var. nordica with a posterior tail, were observed during summer and fall in the hypertrophic lake, lake kasumigaura, japan. the tail shortened in mesocosms stocked with planktivorous silver carp ( hypophthalmichthys molitrix val.) and elongated by more than 50% in mesocosms from which silver carp were removed. the density of plagioselmis cells increased significantly upon fish stocking and decreased upon fish removal. the tail ... | 2003 | 12820066 |
| immunohistochemical study of the endocrine cells in the pancreas of the carp, cyprinus carpio (cyprinidae). | the regional distribution and relative frequency of some endocrine cells in the pancreas of the carp, cyprinus carpio linnaeus, belonging to the family cyprinidae in the order cypriniformes, were observed using specific mammalian antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (hpp) by peroxidase antiperoxidase (pap) method. the pancreas was divided into four regions (principal and secondary islets, exocrine and pancreatic duct regions). in addition, the pancrea ... | 2002 | 12819380 |
| expansion of genes encoding complement components in bony fish: biological implications of the complement diversity. | the complement system is a major humoral component of vertebrate defenses for tagging and killing target microorganisms. recent molecular analyses have uncovered a striking feature of bony fish complement, namely that several complement components are encoded by multiple genes. in this review, the structural diversity of c3, c4, c5, factor b, c2, c1r/s and masp are discussed with special reference to their functional differentiation, mainly focusing on the common carp (cyprinus carpio), a tetrap ... | 2003 | 12818633 |
| identification and characterization of a cell cycle and apoptosis regulatory protein-1 as a novel mediator of apoptosis signaling by retinoid cd437. | cd437, a novel retinoid, causes cell cycle arrest and apoptosis in a number of cancer cells including human breast carcinoma (hbc) by utilizing an undefined retinoic acid receptor/retinoid x receptor-independent mechanism. to delineate mediators of cd437 signaling, we utilized a random antisense-dependent functional knockout genetic approach. we identified a cdna that encodes approximately 130-kda hbc cell perinuclear protein (termed carp-1). treatments with cd437 or chemotherapeutic agent adria ... | 2003 | 12816952 |
| [effect of adrenaline on amylolytic activity of intestinal mucose, glycemia level, glycogen concentration in tissues of carp carassius auratus and carp carassius carassius]. | 2013 | 12815969 | |
| autoradiographic distribution of neuropeptide y binding sites in the brain of the carp cyprinus carpio l. (cyprinidae, teleostei). | the present study reports the distribution of neuropeptide y (npy)-binding sites in the brain of the adult carp cyprinus carpio l. radioiodinated npy was used as tracer in the autoradiographic procedure. the npy-binding sites (npy-bs) were widely distributed in the carp brain. generally, a good match was observed between the distribution of npy-bs and the distribution of npy-immunoreactive (npy-ir) elements previously reported in the forebrain of the carp. low to moderate concentration of npy-bs ... | 2003 | 12814784 |
| physicochemical and physiological properties of 5alpha-cyprinol sulfate, the toxic bile salt of cyprinid fish. | 5alpha-cyprinol sulfate was isolated from bile of the asiatic carp, cyprinus carpio. 5alpha-cyprinol sulfate was surface active and formed micelles; its critical micellization concentration (cmc) in 0.15 m na+ using the maximum bubble pressure device was 1.5 mm; by dye solubilization, its cmc was approximately 4 mm. at concentrations >1 mm, 5alpha-cyprinol sulfate solubilized monooleylglycerol efficiently (2.1 molecules per mol micellar bile salt). when infused intravenously into the anesthetize ... | 2003 | 12810826 |
| subcellular distribution of ryanodine receptors in the cardiac muscle of carp (cyprinus carpio). | we examined the subcellular localization of ryanodine receptors (ryr) in the cardiac muscle of carp using biochemical, immunohistochemical, and electron microscopic methods and compared it with those of rats and guinea pigs. to achieve this goal, an anti-ryr antibody was newly raised against a synthetic peptide corresponding to an amino acid sequence that was conserved among all sequenced ryrs. western blot analysis using this antibody detected a single ryr band following the sds-page of sarcopl ... | 2003 | 12805094 |
| immunocytochemical characterization of adenohypophyseal cells in the greater weever fish (trachinus draco). | the adenohypophysis of the greater weever fish (trachinus draco) was studied using histochemical and immunocytochemical methods. the adenohypophysis comprised the rostral pars distalis (rpd), the proximal pars distalis (ppd), and the pars intermedia (pi). neurohypophysis showed a patent hypophyseal stalk which was divided into several branches intermingled with the adenohypophysis. salmon prolactin (prl)-immunoreactive (ir) cells, arranged in follicles, resided in the rpd and the most rostral pa ... | 2003 | 12798126 |
| vitellogenin in carp (cyprinus carpio) and perch (perca fluviatilis): purification, characterization and development of an elisa for the detection of estrogenic effects. | vitellogenin (vtg), a phospholipoglycoprotein precursor of egg yolk is synthesized and secreted in the liver in response to circulating estrogens in female fish. thus, the presence of vtg in male fish is a useful biomarker to identify estrogenic activity of natural or anthropogenic substances in sewage effluents. we report the purification of carp (cyprinus carpio) and perch (perca fluviatilis) vtg with the subsequent development and characterization of a specific elisa for vtg measurement. vtg ... | 2003 | 12798095 |
| flow cytometric analysis of the neutrophil respiratory burst of ayu, plecoglossus altivelis: comparison with other fresh water fish. | neutrophils of vertebrates undergo respiratory burst activity (rba) as a defense mechanism against bacterial infections. we report here that ayu (plecoglossus altivelis) have unusually high rbas even when they are in a healthy condition. kidney and blood leukocytes were obtained from ayu, rainbow trout (oncorhynchus mykiss), carp (cyprinus carpio), eel (anguilla japonica), and pond smelt (hypomesus nipponensis). neutrophil rba was measured by flow cytometry using dihydrorhodamine after stimulati ... | 2003 | 12787685 |
| [cross reactivity between fish and shellfish]. | in spain, fish allergy represents 18 % of all cases of food allergy in children while reactions caused by crustacea and mollusks account for 3.8 % and 1.6 % respectively. cross-reactivity is defined as the recognition of distinct antigens by the same ige antibody, demonstrable by in vivo and in vitro tests, which clinically manifests as reactions caused by antigens homologous to different species. subclinical sensitization can also occur, giving rise to patients sensitized to particular fish or ... | 2009 | 12783765 |
| acetylcholinesterase and butyrylcholinesterase activities in brain and plasma of freshwater teleosts: cross-species and cross-family differences. | brain and plasma acetylcholinesterase (ache; ec 3.1.1.7) and plasma butyrylcholinesterase (bche; ec 3.1.1.8) specific activities were assayed in 16 freshwater teleosts belonging to four families: cyprinidae, percidae, esocidae and lotidae. brain ache activity varied among fish species approximately 15-fold, ranging from 138 to 2011 micromol/g per h. all cyprinids had higher brain ache activity than other fish families. plasma ache activity was on average 100-fold lower than that in brain, varyin ... | 2003 | 12781973 |
| regulation of branchial na(+)/k(+)-atpase in common carp cyprinus carpio l. acclimated to different temperatures. | isogenic carp cyprinus carpio l. were acclimated to water temperatures of 15, 22 and 29 degrees c for at least 8 weeks. the acclimations consistently resulted in slightly, but significantly, different plasma osmolality, sodium, potassium and chloride concentrations between the groups studied. plasma total and ionic calcium levels were unaffected, indicating successful adaptation. the apparent changes in set point for plasma ion levels are explained by altered sodium pump activity and hormonal co ... | 2003 | 12771175 |
| does the lateral bundle of the medial olfactory tract mediate reproductive behavior in male crucian carp? | the olfactory tract in crucian carp (carassius carassius) is divided into three distinct bundles: the lateral tract (lot) and the lateral (lmot) and medial (mmot) bundles of the medial tract. the lot has been shown to mediate information associated with feeding behavior, whereas the mmot mediates information associated with alarm response. the role of the medial olfactory tract (lmot and mmot) in reproductive behavior is still under debate. in the present experiment, male reproductive behavior t ... | 2003 | 12771016 |
| carp hepatopancreatic dnase i: biochemical, molecular, and immunological properties. | a survey of dnase i in nine different carp tissues showed that the hepatopancreas has the highest levels of both dnase i enzyme activity and gene expression. carp hepatopancreatic dnase i was purified 17,000-fold, with a yield of 29%, to electrophoretic homogeneity using three-step column chromatography. the purified enzyme activity was inhibited completely by 20 mm edta and a specific anti-carp dnase i antibody and slightly by g-actin. histochemical analysis using this antibody revealed the str ... | 2003 | 12761174 |
| detection of myxobolus rotundus (myxozoa: myxosporea) in skin mucus of crucian carp carassius auratus auratus using a monoclonal antibody. | diagnosis of myxosporean myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp carassius auratus auratus with a monoclonal antibody, mab 2d12, raised previously against the parasite. a positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the mab 2d12. it was also demonstrated that m. rotundus infection can be successfully detected by a simple method, enzyme-linked ... | 2003 | 12747643 |
| parasitic infections in freshwater ornamental fish in sri lanka. | a total of 1520 ornamental fish of 13 species from 26 export farms in sri lanka were collected between october 1999 and march 2000 and examined for parasites. fish species examined were guppy poecilia reticulata, goldfish carassius auratus, platy xiphophorus maculatus, molly poecilia sphenops, angel pterophyllum scalare, swordtail xiphophorus helleri, tetras hyphessobrycon species, barbs capeota and puntius spp., gourami colisa sp., carp cyprinus carpio, fighters betta spelendens and others (bra ... | 2003 | 12747641 |
| cardiac-restricted ankyrin-repeated protein is differentially induced in duchenne and congenital muscular dystrophy. | cardiac ankyrin-repeated protein (carp) has been shown to associate with a transcription factor, yb-1, that may activate expression of the ventricular myosin light chain-2 gene during cardiogenesis. carp is induced in the adult hypertrophic heart subjected to pressure overload, suggesting that carp may play important functional roles in both embryonic and adult hearts. although carp expression was initially believed to be restricted to the heart, we found recently that carp is induced strongly i ... | 2003 | 12746480 |
| dopamine effect on the stimulus pattern related changes in response characteristics of r/g horizontal cells in carp retina. | repetitive red flashes increased the r/g horizontal cells' red response amplitude and induced a hyperpolarization of the cells' dark membrane potential. these phenomena were eliminated in 6-ohda pretreated retinas and restored by exogenous dopamine, which suggests the involvement of dopamine receptor activity changes instead of dopamine release changes. furthermore, the phenomena persisted on d(1) receptor antagonist (skf-83566) application, whereas they diminished on d(2) receptor antagonist (e ... | 2003 | 12738062 |
| functional changes of the lh-immunoreactive cells in adenohypophysis of the common carp (cyprinus carpio) from rivers contaminated with estrogenic chemicals. | the adverse effect of estrogenic chemicals on luteinizing hormone-immunoreactive (lh-ir) cells in the adenohypophysis of common carp (cyprinus carpio) was examined using immunocytochemical and morphometric methods. adult male fish were collected from two contaminated sites (ishizu and wada rivers) and from a control pond at agriculture, food and environmental sciences, research center of osaka prefecture. the concentration of nonylphenol, bisphenol a and 17beta-estradiol in the ishizu river was ... | 2003 | 12736431 |
| adenophostin a and imipramine are two activators of the olfactory inositol 1,4,5-trisphosphate-gated channel in fish olfatory cilia. | binding of an odorant to its receptor activates the camp-dependent pathway, and also leads to inositol 1,4,5-trisphosphate (insp(3)) production. this induces opening of a plasma membrane channel in olfactory receptor cells (orcs). we investigated single-channel properties of this channel in the presence of a phospholipase c (plc) activator (imipramine) and of a potent activator of the insp(3)/ca(2+) release channel (adenophostin a) by reconstituting carp olfactory cilia into planar lipid bilayer ... | 2003 | 12734698 |
| screening toxicity study in young carp (cyprinus carpio l.) on feed amended with fumonisin b1. | fumonisin b1 (fb1) is one of several mycotoxins produced by fusarium moniliforme, a major fungal pathogen of corn and widely spread throughout the world. fb1 produces a wide range of biological effects, some of which are specific for particular organs or species and some are common to all investigated animals. in this study we have evaluated subchronic toxicosis features in young carp (cyprinus carpio l.) exposed to 0.5 and 5.0 mg fb1 kg(-1) body weight for 42 days through nutritionally balanced ... | 2003 | 12733635 |
| investigation of acute toxicity and the effect of 2,4-d (2,4-dichlorophenoxyacetic acid) herbicide on the behavior of the common carp (cyprinus carpio l., 1758; pisces, cyprinidae). | a 96-h lc(50) values of 2,4-d [(2,4-dichlorophenoxy)acetic acid], a common contaminating agricultural herbicide, were determined on the adult common carp (cyprinus carpio l., 1758; pisces, cyprinidae). the study was conducted in two stages using 130 carp. the data obtained were statistically evaluated by the use of the epa computer program based on finney's probit analysis method and a 96-h lc(50) value for c. carpio l., 1758 was found to be 63.24 mg/l in a static bioassay test system. 95% lower ... | 2003 | 12729702 |
| effect of cortisol treatment and/or sublethal copper exposure on copper uptake and heat shock protein levels in common carp, cyprinus carpio. | this study compared the effects of increased endogenous cortisol levels and/or sublethal cu exposure on cu accumulation and stress protein levels (hsp70) in the freshwater common carp, cyprinus carpio. fish were exposed to either increased levels of endogenous cortisol (200 ng/ml) or sublethal cu concentrations (1.9 microm, approximately 20% of the acute 96-h median lethal concentration [lc50]) alone or were pretreated by elevating plasma cortisol levels prior to cu exposure to evaluate whether ... | 2003 | 12729223 |
| molecular cloning and expression analysis of a novel caspase recruitment domain protein (card) in common carp cyprinus carpio l. | a novel caspase recruitment domain protein (card) was isolated from common carp cyprinus carpio l. by expressed sequence tag analysis. this gene consist of a 2016 bp open reading frame and untranslated regions, which is putatively translated to a protein of 535 amino acid residues. the gene harbors domains (card and coiled-coil domain), which are conserved in proteins of card family. the card domain have carp was similar to human card9 with 72.4% identity. expression analysis revealed that card ... | 2003 | 12727358 |
| metabotropic glutamate receptor-mediated hetero-synaptic interaction of red- and green-cone inputs to lhc of carp retina. | the role of presynaptic metabotropic glutamate receptor (mglur) on the interaction of red- and green-cone signals was investigated in luminosity-type horizontal cell (lhc) of isolated carp retina. it was found that a dim red background could enhance lhc's light response to green stimulus, and a dim green background was also able to increase the cell's response to red flash. such mutual color enhancement was eliminated by application of groups ii and iii mglur antagonist (s)-methyl-4-carboxypheny ... | 2003 | 12725894 |
| [the budget model of ecosystem of a shallow highly eutrophic lake]. | a model of energy budget of lake bolshoi okunenok ecosystem was based on the data received during field studies from may through november 1986. the model takes into account 36 components including dissolved organic matter, bacteria, phytoplankton, zooplankton, meiobenthos, macrobenthos, fish, suspended and sediment detritus. the growing season has been divided into 16 intervals according to the number of observations. the balance equation for each live component describes the change in its bioma ... | 2006 | 12723370 |
| a study of 110mag in aquatic and terrestrial ecosystems. | experiments on a simulated terrestrial agricultural ecosystem were carried out using the pot culture approach. the most representative plants in local vegetable gardens were selected to investigate the root uptake of (110m)ag. the results show that carrot, kale and flowering cabbage have the largest transfer factor values among the vegetables. flowering cabbage, as the most popular leafy vegetable in hong kong and the south china area, can be used as a biomonitor for radioisotope contamination i ... | 2003 | 12720001 |
| effect of sub-lethal concentrations of endosulfan on hematological and serum biochemical parameters in the carp cyprinus carpio. | 2003 | 12719826 | |
| sensitivity and selectivity of neurons in the medial region of the olfactory bulb to skin extract from conspecifics in crucian carp, carassius carassius. | to examine the functional subdivision of the teleost olfactory bulb, extracellular recordings were made from the posterior part of the medial region of the olfactory bulb in the crucian carp, carassius carassius. bulbar units classified as type i or type ii were frequently and simultaneously encountered at a recording site. type i units displayed a diphasic action potential (ap) with a relatively small amplitude, a short duration (rise time approximately 1 ms) and high spontaneous activity (2.5 ... | 2003 | 12714440 |
| 3-methylcholanthrene inhibits lymphocyte proliferation and increases intracellular calcium levels in common carp (cyprinus carpio l). | polycyclic aromatic hydrocarbons (pahs) are an important class of environmental pollutants that are known to be carcinogenic and immunotoxic. many authors have focused on macrophage activities in fish exposed to pahs. however, fewer studies have reported decrease in specific immunity in such fish. we investigated the intracellular mechanisms by which the 3-methylcholanthrene (3-mc) decreased lymphocyte proliferation in carp. t- and b-lymphocyte proliferation induced by concanavalin a (con a) and ... | 2003 | 12711420 |
| head kidney neutrophils of carp (cyprinus carpio l.) are functionally modulated by the haemoflagellatetrypanoplasma borreli. | in the present work responses of carp (cyprinus carpio) head kidney-derived neutrophils to the blood parasite t. borreli, and the consequences of these responses for parasite survival and other host response mechanisms, were studied. in co-cultures of head kidney leucocytes (hkl) with viable and lysed t. borreli a prominent shape change of neutrophilic granulocytes towards increased size and complexity was observed. in addition, the longevity of neutrophils in vitro was prolonged in the presence ... | 2003 | 12711273 |
| occurrence of monogenea in italian freshwater fish (po river basin). | sixteen monogenean species were found on the skin and gills of 10 different fish species collected from north italian water bodies. cleidodiscus pricei from ictalurus melas; dactylogyrus alatus from alburnus alburnus; d. anchoratus, d. formosus and d. vastator from carassius carassius; d. difformis from scardinius erythrophthalmus; d. rutili from rutilus aula; d. tincae and gyrodactylus gasterostei from tinca tinca; d. ergensi from leuciscus cephalus and telestes muticellus; d. vistulae and para ... | 2002 | 12701382 |
| different capacities of carp leukocytes to encounter nitric oxide-mediated stress: a role for the intracellular reduced glutathione pool. | carp head kidney (hk) phagocytes can be stimulated by lipopolysaccharide (lps) to produce nitric oxide (no). high production of no can suppress the carp immune system. carp peripheral blood leukocytes (pbl) are highly susceptible but hk phagocytes are relatively resistant to the immunosuppressive effects of no. this study demonstrates that the antioxidant glutathione plays an important role in the protection against nitrosative stress. carp hk phagocytes, especially the neutrophilic granulocytes ... | 2013 | 12697312 |
| characterization of carbonic anhydrases from riftia pachyptila, a symbiotic invertebrate from deep-sea hydrothermal vents. | the symbiotic hydrothermal vent tubeworm riftia pachyptila needs to supply its internal bacterial symbionts with carbon dioxide, their inorganic carbon source. our aim in this study was to characterize the carbonic anhydrase (ca) involved in co(2) transport and conversion at various steps in the plume and the symbiotic tissue, the trophosome. a complete 1209 kb cdna has been sequenced from the trophosome and identified as a putative alpha-ca based on blast analysis and the similarities of total ... | 2003 | 12696045 |
| transduction of cultured fish cells with recombinant baculoviruses. | five fish cell lines were tested for their ability to be transduced by ac-calacz, a recombinant baculovirus that is capable of expressing a beta-galactosidase reporter gene from the cag promoter (consisting of a cytomegalovirus enhancer element, a chicken actin promoter and rabbit beta-globin termination sequences). to (tilapia ovary), epc (carp), chh-1 (chum salmon heart fibroblast) and chse-214 (chinook salmon embryo) cells were transducible, as demonstrated by an in situ beta-galactosidase as ... | 2003 | 12692282 |
| detection and genetic analysis of group ii capsules in aeromonas hydrophila. | the genetic organization and sequences of the group ii capsule gene cluster of aeromonas hydrophila ppd134/91 have been determined previously. the purified capsular polysaccharides can increase the ability of avirulent strain ppd35/85 to survive in naive tilapia serum but have no inhibitory effect on the adhesion of ppd134/91 to carp epithelial cells. in this study, the presence of group ii capsules among 33 randomly chosen a. hydrophila strains was examined by electron microscopy and genetic an ... | 2003 | 12686647 |
| [endurance ability of transgenic common carp under different environmental conditions]. | this paper dealt with the endurance ability of transgenic common carp f3, and common carp under different environmental factors such as temperature, alkaline, and ph value. the results showed that there was no obvious difference of endurance abilities between transgenic common carp and common carp under the different environmental factors. | 2002 | 12682991 |
| local expression of apolipoprotein a-i gene and a possible role for hdl in primary defence in the carp skin. | antimicrobial proteins and peptides play an important role in the primary defence of epithelial barriers in vertebrates and invertebrates. here we report the detection of the apolipoproteins a-i and a-ii in the epidermis and epidermal mucus of the carp (cyprinus carpio l.) by immunohistochemistry and western blot analysis. both apolipoproteins are major constituents of high density lipoprotein and have been shown to display antiviral and antimicrobial activity in mammals. therefore the aim of th ... | 2003 | 12681280 |
| the haemoflagellate trypanoplasma borreli induces the production of nitric oxide, which is associated with modulation of carp (cyprinus carpio l.) leucocyte functions. | in an attempt to characterise the role of nitric oxide (no) in immune responses of carp, carp leucocytes obtained during an acute t. borreli infection were examined, for their capacity to generate no. in a second set of experiments the impact no on viability of the parasite and on the modulation of functional carp leucocyte responses were tested in vitro. both in carp head-kidneys and in the peripheral blood, the fractions of lymphoblasts among separated leucocytes were increased. however, the r ... | 2003 | 12681277 |
| aquatic hypoxia is an disrupter and impairs fish reproduction. | there is increasing concern that certain chemicals in the aquatic environment can disrupt endocrine systems, leading to reproductive impairment and threatening survival of wild populations of invertebrates, fish, bird, reptiles, and wildlife. for the first time, we report that hypoxia is also an endocrine disruptor and poses a significant threat to the reproduction and hence sustainability of fish populations. serum levels of testosterone, estradiol, and triiodothyronine significantly decreased ... | 2003 | 12680666 |
| altered expression of cardiac ankyrin repeat protein and its homologue, ankyrin repeat protein with pest and proline-rich region, in atrophic muscles in amyotrophic lateral sclerosis. | cardiac ankyrin repeat protein, carp, is a protein that is restrictedly expressed in the heart but barely expressed in skeletal muscles. since carp is induced by pressure overload to the heart, it is proposed to be a genetic marker for cardiac hypertrophy. we recently identified a novel protein, ankyrin repeat protein with pest and proline-rich region (arpp), which is homologous to carp and is preferentially expressed in type 1 skeletal muscle fibers (cf. slow fibers). we also found that both ar ... | 2002 | 12679596 |
| influence of methisoprinol on the replication of rhabdoviruses isolated from carp (cyprinus carpio) and catfish (ictalurus melas): in vitro study. | rhabdoviruses constitute one of the most pathogenic viruses isolated from rainbow trout and carp culture. several viruses were also isolated from other species of fish. these viruses are mostly associated with epizootics and heavy losses. spring viraemia of carp virus (svcv) and pike fry rhabdovirus (pfrv) have been the most extensively studied, due to their significant economic impact. significant progress has been made towards controlling the major bacterial fish diseases using vaccines, but t ... | 2003 | 12675468 |
| ryanodine and dihydropyridine receptor binding in ventricular cardiac muscle of fish with different temperature preferences. | ca(2+)-induced ca2+ release (cicr) mechanism of cardiac excitation-contraction (e-c) coupling is dependent on the close apposition between the sarcolemmal dihydropyridine receptors (dhpr) and the sarcoplasmic reticulum (sr) ryanodine receptors (ryr). in particular, high ryr/dhpr ratio is considered to reflect strong dependence on sr ca2+ stores for the intracellular ca2+ transient. to indirectly evaluate the significance of cicr in fish hearts, densities of cardiac dhprs and ryrs were compared i ... | 2003 | 12664089 |
| in vitro modulation of common carp (cyprinus carpio l.) phagocytic cells by di-n-butyl phthalate and di-2-ethylhexyl phthalate. | the modulation of common carp, cyprinus carpio l. phagocytic cells by di-n-butyl phthalate (dbp) and di-2-ethylhexyl phthalate (dehp) were analyzed in vitro. carp head kidney leucocytes were cultured in a rpmi 1640 medium containing dbp or dehp at concentration of 1-1000 nm and the production of superoxide anion was measured via the in vitro reduction of nitroblue tetrazolium (nbt). macrophages incubated with 100 up to 1000 nm of dbp showed an increase in their production of superoxide anion in ... | 2003 | 12657487 |
| potencies of estrogenic compounds in in vitro screening assays and in life cycle tests with zebrafish in vivo. | the objective of this study was to compare the estrogenic potency of environmental estrogens at two testing tiers: at the initial level of in vitro screening assays, and at the level of definitive fish reproduction tests in vivo. the in vitro tests included a recombinant yeast estrogen receptor (er) assay, a competitive radioreceptor assay using the hepatic er of carp (cyprinus carpio), and assays on vitellogenin induction in cultured hepatocytes of rainbow trout (oncorhynchus mykiss) and carp. ... | 2003 | 12651187 |
| ultrastructure of carp cyprinus carpio permatozoa after cooling, dilution and freeze-thawing. | dilution of the carp spermatozoa with a cryoprotective medium was shown to inflict serious injuries to the fine structures of some spermatozoa. the amount of the injured spermatozoa increases after cryopreservation. examination of the freeze-fracture micrographs of the heads of the carp spermatozoa, showed that their protoplasmic faces were covered with the particles in high concentrations. these particles do not form clusters after cooling the spermatozoa down to +5 degrees c and dilution with ... | 2006 | 12644853 |
| a new cell secreting insulin. | the pancreatic beta-cell is the only cell in animals that expresses the insulin gene and secretes insulin protein. we have found copious release of immunoreactive and bioactive insulin into the medium from the primary culture of carp adipocytes. glucose augmented this release to more than 2-fold, and glucose transporter, glut2, was detected in these cells. these all reflect characteristics of a pancreatic beta-cell. the expression of the adipocyte-specific flotillin gene, the presence of peroxis ... | 2003 | 12639943 |
| effect of a non-hormonal feed additive, cholymbi on growth, body composition and digestive enzyme activity of common carp, cyprinus carpio. | supplementation of pelleted feeds (30% crude protein) consisting of practical ingredients with 250, 375 and 500 mg cholymbi/kg diet resulted significantly improved the growth of common carp, cyprinus carpio. highest final average weight was recorded with diet containing 500 mg cholymbi/kg. feeding cholymbi to fish showed increased carcass protein and fat. gut protease and hepatopancreas amylase activity were the highest with 500 mg cholymbi/kg incorporated diet (t3). the results of the present s ... | 2002 | 12635714 |
| humoral immune response of carp (cyprinus carpio) induced by oral immunization with liposome-entrapped antigen. | to study the value of liposomes as carriers of antigens for oral vaccination in fish, humoral immune responses were analyzed after immunizing carp (cyprinus carpio) with liposome-entrapped bovine serum albumin (bsa) as a model antigen. oral immunization of bsa (100 microg)-containing liposomes that were stable in carp bile induced significant antibody responses against bsa in serum as well as in intestinal mucus and bile. by contrast, no serum antibody responses were observed when fish were oral ... | 2003 | 12631523 |
| a double staining flow cytometric assay for the detection of steroid induced apoptotic leucocytes in common carp (cyprinus carpio). | steroid hormones play an important role in the regulation of the immune system through different ways. in this in vitro study, the effects of steroid hormones on the apoptosis of leucocytes were evaluated to understand the involvement of this process in the immunocompetence of common carp. prior to the investigation, a double staining flow cytometric assay using fluorescein diacetate (fda), which reacts with esterases of viable cells, and propidium iodide (pi), an acid dye that binds with nuclea ... | 2003 | 12631518 |
| amylolytic activity in fish intestinal mucosa: temperature effects. | the activity, temperature characteristics and energy of activation of amylolytic enzymes in the intestinal mucosa were studied in six species of fish living in a boreal zone [burbot (lota lota l.), northern pike (exos lucius l.), perch (perca fluviatilis l.), bream (abramis brama l.), roach (rutilis rutilis l.), and carp (cyprinus carpio l.)] and in three species from tropical and subtropical areas [pilchard (sardina pilchardus w.), jack mackerel (trachurus trecae c.) and round sardinella (sardi ... | 2003 | 12628384 |
| enzyme activities in fish spermatozoa with focus on lactate dehydrogenase isoenzymes from herring clupea harengus. | the activities of nad- and nadp-dependent dehydrogenases and creatine kinase were compared in extracts of spermatozoa from herring (clupea harengus), carp (cyprinus carpio) and catfish (clarias gariepinus). the activity of malic enzyme in herring spermatozoa was approximately 5 and 36 times higher than in carp and catfish spermatozoa. in contrast, lactate dehydrogenase activity in herring spermatozoa was very low. herring spermatozoa possess two isoenzymes of lactate dehydrogenase: ldh-a(2)b(2) ... | 2003 | 12628371 |
| reproduction mode of an artificial allotetraploid carp (pisces; cyprinidae). | mature eggs of allotetraploid carp were activated by inactive sperm or crossed with normal sperms of common carp (cyprinus carpio), crucian carp (carassius auratus), chinese blunt snout bream (megalobrama amblycephala), hemiculter leucisculus and pseudorasbora parva. chromosome counts showed that all offspring of these crosses presented a mode number of 200 chromosomes (4n = 200), and their morphological traits are much like maternal. microsatelite marker and rapd patterns between allotetraploid ... | 2002 | 12627840 |
| contamination of fish in streams of the mid-atlantic region: an approach to regional indicator selection and wildlife assessment. | the extent of contamination of fish in the mid-atlantic region was evaluated as part of the u.s. environmental protection agency's monitoring and assessment program's regional assessment in 1993 through 1994. fish assemblages from wadeable streams were dominated by small, short-lived fishes (e.g., minnows, darters, and sculpins) that were more widely distributed and abundant than large fishes typically chosen for tissue contaminant studies (e.g., trout, black bass, sunfish, common carp). chemica ... | 2003 | 12627641 |
| temperature-induced changes in thyrotropin-releasing hormone sensitivity in carp melanotropes. | this study investigates whether thyrotropin-releasing hormone (trh), alpha-melanocyte-stimulating hormone (alpha-msh) and n-acetyl beta-endorphin (nac beta-end), or the thyroid hormones thyroxine (t4) and 3,5,3'-triiodothyronine (t3) are involved in the physiological response to temperature changes in the poikilotherm common carp (cyprinus carpio). carps were either subjected to a rapid cold exposure or acclimated over time to three different temperatures. acute cold exposure did not influence b ... | 2003 | 12624537 |
| immunocytochemical studies on the pituitary gland of anguilla anguilla l., in relation to early growth stages and diet-induced sex differentiation. | mammalian and teleost antisera against pituitary hormones were used to identify and localize pituitary cell types in the european eel (anguilla anguilla l.). the investigation was conducted on unpigmented glass eels of 5.6-6.2 of total body length (l(t)) caught in river mouths, then on yellow eels reared from the pigmented glass eel (or elver) stage up to 12-14 cm of l(t), in an eel farm in warm freshwater. treated elvers were fed with commercial paste food supplemented with mature carp ovaries, ... | 2003 | 12620249 |
| the complement component c5 of the common carp (cyprinus carpio): cdna cloning of two distinct isotypes that differ in a functional site. | the complement component c5 plays important roles in inflammatory responses and complement-mediated cytolysis. in bony fish, although c5 has been identified at the dna or the protein level in trout, carp and gilthead seabream, only partial c5 sequences are available. the present study was designed to obtain the complete primary structure of c5 from the common carp ( cyprinus carpio) and to examine its possible structural diversity. reverse-transcribed polymerase chain reaction amplification from ... | 2003 | 12618914 |
| molecular cloning of the complement regulatory factor i isotypes from the common carp (cyprinus carpio). | factor i is a novel serine protease that regulates complement activation. here we report the complete primary structure of two isotypic factor is isolated from the common carp ( cyprinus carpio), a pseudotetraploid teleost. a carp hepatopancreas cdna library was screened using two rt-pcr-amplified cdna fragments encoding part of the carp factor i-like serine protease domain. two distinct cdna clones, designated fi-a and fi-b, were isolated. their deduced amino acid sequences share 75.2% identity ... | 2003 | 12618913 |
| isolation and characterization of a novel cxc chemokine in common carp (cyprinus carpio l.). | a novel cxc chemokine was identified for the first time in fish from common carp (cyprinus carpio l.). the gene was obtained from the head kidney (hk) stimulated with lps and con a. the cdna consists of 619 bp with a 37 bp 5' utr and a 287 bp 3' utr. an open reading frame of 368 bp encodes a 97 amino acid peptide, with a putative signal peptide of 20 aa. the gene has four cysteines residues, which are conserved, with first two cysteines separated with phenylalanine. by homology and phylogenetic ... | 2003 | 12617998 |
| cytochrome p450 induction and gonadal status alteration in common carp (cyprinus carpio) associated with the discharge of dioxin contaminated effluent to the hikiji river, kanagawa prefecture, japan. | accumulations of polychlorinated dibenzo-p-dioxins, dibenzofurans and coplanar polychlorinated biphenyls were analyzed in common carp (cyprinus carpio) collected in the hikiji river, kanagawa prefecture, japan in which dioxin contaminated effluent was released during the period starting from november 1992 to march 2000. higher levels of 2,3,7,8-tetrachlorinated dibenzo-p-dioxin toxic equivalents were observed in carps collected downstream to the dioxin release site (contaminated site) than the r ... | 2003 | 12615101 |
| carbonic anhydrase-related protein is a novel binding protein for inositol 1,4,5-trisphosphate receptor type 1. | the inositol 1,4,5-trisphosphate (ip(3)) receptor (ip(3)r) is an intracellular ip(3)-gated ca(2+) channel that is located on intracellular ca(2+) stores and modulates ca(2+) signalling. using the yeast two-hybrid system, we screened a mouse brain cdna library with bait constructs for mouse ip(3)r type 1 (ip(3)r1) to identify ip(3)r1-associated proteins. in this way, we found that carbonic anhydrase-related protein (carp) is a novel ip(3)r1-binding protein. western blot analysis revealed that car ... | 2003 | 12611586 |
| genomic structure, expression and characterization of a stat5 homologue from pufferfish (tetraodon fluviatilis). | the stat5 (signal transducer and activator of transcription 5) gene was isolated and characterized from a round-spotted pufferfish genomic library. this gene is composed of 19 exons spanning 11 kb. the full-length cdna of tetraodon fluviatilis stat5 (tfstat5) contains 2461 bp and encodes a protein of 785 amino acid residues. from the amino acid sequence comparison, tfstat5 is most similar to mouse stat5a and stat5b with an overall identity of 76% and 78%, respectively, and has < 35% identity wit ... | 2003 | 12605675 |
| genotoxicity of a polluted river system measured using the alkaline comet assay on fish and earthworm tissues. | monitoring genotoxicity in the environment by using endemic organisms as sentinels requires sensitive assays. in this study the genotoxic properties of water and sediment collected from the noyyal river, which is polluted with industrial effluent and sewage, was determined in fish (cyprinus carpio) and earthworms (eisenia foetida) using the alkaline comet assay. upon electrophoresis, extensive dna damage, measured as the dna length:width ratio of the dna mass, was observed in erythrocytes, liver ... | 2003 | 12605376 |
| impact of cadmium on food utilization of the indian major carp, catla catla (ham). | catla catla, under the sublethal stress of cadmium exhibited depletion in food utilization parameters and it was concentration dependent. heavy metal intoxication was found to exhibit reduction in biomass. | 2002 | 12602860 |
| the toxicity of ammonia, nitrite and nitrate to the fish, catla catla (hamilton). | the acute toxicity of unionized ammonia; nitrite and nitrate to the indian major carp catla catla (hamilton) was determined using static and continuous flow through systems for 24 hours. the median lethal concentration (lc50) values for 24 h of ammonia (nh3-n), nitrite (no2-n) and nitrate (no3-n) were 0.045 mg/l, 120.84 mg/l and 1565.43 mg/l in static test respectively and were 0.036 mg/l, 117.43 mg/l and 1484.08 mg/l in continuous flow through test respectively. | 2002 | 12602850 |
| effects of oxytocin on semen release response in african catfish (clarias gariepinus). | in silurid fishes, semen collection is practically impossible, even after hormonal stimulation. instead, males are killed and testes macerated to obtain sperm. to understand the endocrine control of semen release in catfishes, we investigated the role of smooth muscle contractors in semen release and semen quality of african catfish (clarias gariepinus). in in vitro experiments, testis slices were incubated with oxytocin (1 and 10 iu), isotocin (2 and 20 ug), vasopressin (0.2 and 2 ug), epinephr ... | 2003 | 12600728 |
| comparison of elisas for detecting vitellogenin in the fathead minnow (pimephales promelas). | measurement of vitellogenin (vtg) concentrations in the fathead minnow (pimephales promelas) is currently being considered and evaluated for screening of endocrine active substances. one of the proposed methods, an enzyme-linked immunosorbent assay (elisa) based on vtg from carp (cyprinus carpio), was recently evaluated in an inter-laboratory ring test using whole body homogenates from juvenile fathead minnows. the objective of the current study was to compare the performance of three different ... | 2003 | 12600685 |
| carp (cyprinus carpio) vitellogenin: purification and development of a simultaneous chemiluminescent immunoassay. | vitellogenin (vg) was purified from the serum of vitellogenic female carp (cyprinus carpio) by hydroxylapatite column chromatography and gel filtration. vg had an apparent molecular mass of 490 kda and appeared as two bands corresponding to 190 and 156 kda after sds-page under reducing conditions. these bands were immunoreacted in western blotting using antiserum against carp lipovitellin (anti-lv) which is an egg yolk protein derived from vg. the amino acid composition of carp vg was similar to ... | 2003 | 12600670 |
| evaluation of the toxicological effects of perfluorooctane sulfonic acid in the common carp (cyprinus carpio). | in the present study we evaluated the toxicological effects of a scarcely documented environmental pollutant, perfluorooctane sulfonic acid (pfos), on selected biochemical endpoints in the common carp, cyprinus carpio. juvenile organisms were exposed to pfos through a single intraperitoneal injection (liver concentrations ranging from 16 to 864 ng/g after 5 days of exposure) and after 1 and 5 days effects were assessed in liver and serum of the exposed organisms. the investigation of the hepatot ... | 2003 | 12595174 |
| daily handling stress reduces resistance of carp to trypanoplasma borreli: in vitro modulatory effects of cortisol on leukocyte function and apoptosis. | carp subjected to daily handling stress were much more susceptible to trypanoplasma borreli infection than control fish. in a search for the cellular mechanisms involved, it was observed that cortisol suppressed t. borreli-induced expression of interleukin-1beta, tumor necrosis factor-alpha, serum amyloid a and inducible nitric oxide synthase. an nf-kappab-inhibitor could replicate cortisol-induced apoptosis of activated peripheral blood leukocytes. in contrast, although this nf-kappab-inhibitor ... | 2003 | 12590974 |
| unique bioconcentration characteristics of new aryl fluoroalkyl ethers in common carp (cyprinus carpio). | the bioconcentration factors (bcfs) of seven new aryl fluoroalkyl ethers--four bis-4-tetrafluoroethoxyphenyl-type (bis-type) compounds and three mono-4-tetrafluoroethoxyphenyl-type (mono-type) compounds--were obtained by bioconcentration tests using common carp. the bcfs of 4 of the 7 ethers were higher than 5000, indicating their high bioconcentration potential. the bioconcentration characteristics of the bis-type compounds were different from those of the mono-type compounds and non-fluoro dip ... | 2003 | 12586148 |
| isolation and characterization of novel tachykinins from the posterior salivary gland of the common octopus octopus vulgaris. | two novel tachykinins (octtk-i: lys-pro-pro-ser-ser-ser-glu-phe-ile-gly-leu-met-nh(2) and octtk-ii: lys-pro-pro-ser-ser-ser-glu-phe-val-gly-leu-met-nh(2)) were isolated from the posterior salivary gland of the octopus (octopus vulgaris) using a contraction assay of the carp rectum. these peptides had in common the pentapeptide sequence -phe-x-gly-leu-met-nh(2) at the c-terminal and induced immediate contractions on the carp rectum and the guinea-pig ileum. cdnas encoding their precursor proteins ... | 2003 | 12576083 |
| nephropathy associated with animal, plant, and chemical toxins in the tropics. | widespread human exposure to a variety of drugs, chemicals, and biologic products and recent awareness of their toxic manifestations has led to the recognition of toxic nephropathy as an important segment of renal disease in the tropical countries. tropical nephrotoxins are distinctly different from those seen in the rest of the world and are derived from local fauna and flora or plant and chemical sources. the spectrum of exposure varies from country to country and even from community to commun ... | 2003 | 12563601 |
| evidence for presence of gaba-ergic receptor mediated dispersion in isolated scale melanophores of a carp, cirrhinus mrigala ham. | effects of gaba-ergic agonists and antagonists were examined on the melanophores of a carp c. mrigala in vitro. gaba and baclofen both induced concentration - related dispersion in fish melanophores. denervation of the melanophores by reserpine treatment potentiated the sensitivity of the melanophores to gaba. while denervation by cooling treatment inhibited the sensitivity of the melanophores to gaba, atropine, bicuculline and pentylenetetrazole all inhibited the dispersal responses of the mela ... | 2002 | 12561974 |
| responses of isolated scale melanophores of a fresh water carp, cirrhinus mrigala (ham.) to zidovudine. | zidovudine (azt) induced concentration related aggregation in c. mrigala melanophores. denervated melanophores failed to respond to azt. specific and nonspecific alpha adrenoceptor antagonists completely blocked the responses of fish melanophores to azt. histamine and prostaglandin antagonists also inhibited aggregation of the melanophores induced by azt. the results suggest that azt may release a mixture of neurotransmitter like substances, which cause the aggregation of this fish melanophores. | 2002 | 12561973 |
| determination of polychlorinated biphenyl congeners and chlorinated pesticides in a fish tissue standard reference material. | the concentrations of a wide range of polychlorinated biphenyl congeners (pcbs) and chlorinated pesticides in a fish tissue standard reference material (srm) have been determined using multiple methods of analysis. this material, srm 1946, lake superior fish tissue, was recently issued by the national institute of standards and technology (nist) and complements a suite of marine environmental natural-matrix srms that are currently available from nist for the determination of organic contaminants ... | 2003 | 12560966 |
| membrane chromatographic method for the rapid purification of vitellogenin from fish plasma. | a membrane chromatographic method was developed for the rapid purification of vitellogenin (vtg) from the plasma of 17beta-estradiol induced loach (misgurnus angaillicaud atus) and carp (cyprinus carpio). the time required for the proposed procedure is less then 10 min at a flow-rate of 5 ml/min of the mobile phase, and 0.5 ml of fish plasma could be separated in one cycle. multistep gradient elution was more suitable for the separation than linear gradient elution. under optimized conditions, a ... | 2003 | 12554150 |
| spring viremia of carp (svc). | spring viremia of carp (svc) is an important disease affecting cyprinids, mainly common carp cyprinus carpio. the disease is widespread in european carp culture, where it causes significant morbidity and mortality. designated a notifiable disease by the office international des epizooties, svc is caused by a rhabdovirus, spring viremia of carp virus (svcv). affected fish show destruction of tissues in the kidney, spleen and liver, leading to hemorrhage, loss of water-salt balance and impairment ... | 2002 | 12553453 |
| [studies on genetic diversity in gonochoristic offspring produced from mating between two different gynogenetic clones of silver crucian carp]. | based on the discovery of gonochoristic reproductive mode in silver crucian carp (carassius auratus gibelio), 18 individuals from the mated offspring between clone f and clone d and their parents were analyzed using 11 rapd primers. the mated offspring differentiated into three phenotypes. one phenotype is similar to that of clone f (sf). the second is similar to that of clone d (sd). the third shows a novel longer and thinner body type (nl). electrophoretogram results show abundant polymorhic d ... | 2001 | 12549217 |
| feeding mechanisms in carp: crossflow filtration, palatal protrusions and flow reversals. | it has been hypothesized that, when engulfing food mixed with inorganic particles during benthic feeding, cyprinid fish use protrusions of tissue from the palatal organ to retain the food particles while the inorganic particles are expelled from the opercular slits. in crossflow filtration, the particle suspension is pumped parallel to the filter surface as filtrate exits through the filter pores, causing the suspension to become more concentrated as it travels downstream along the filter. we us ... | 2003 | 12547943 |
| unusual physiology of scale-less carp, gymnocypris przewalskii, in lake qinghai: a high altitude alkaline saline lake. | the scale-less carp (gymnocypris przewalskii) inhabits lake qinghai located on the qinghai-tibet plateau (elevation, 3200 m) in western china. the lake waters are alkaline (ph 9.4, titratable alkalinity=30 mmol l(-1)), mg(2+)-rich (18.7 mmol l(-1)), ca(2+)-poor (0.30 mmol l(-1)) and saline (9 per thousand ). these fish make annual spawning migrations into freshwater rivers. we investigated the physiology of nitrogen excretion and ionoregulation of fish from the lake and river. fish from both wat ... | 2003 | 12547271 |
| cloning and expression of a novel neurotrophin, nt-7, from carp. | neurotrophins have been demonstrated to play important roles in the development and functioning of the nervous system. this family of proteins consists of four homologous members in mammals: ngf, bdnf, nt-3, and nt-4/5. a new member, called nt-6, was recently cloned from the platyfish xiphophorus maculatus. this protein shares closer structural relationship to ngf than the other neurotrophins, but contains a characteristic insertion of 22 amino acids that constituted the heparin-binding domain. ... | 1998 | 12546061 |
| immunomodulatory effects of alpha melanocyte stimulating hormone on common carp (cyprinus carpio l.). | the immunomodulatory effects of phagocytic cells in common carp cyprinus carpio l., by alpha melanocyte stimulating hormone (alpha-msh) was analyzed in vitro. carp head kidney leucocytes were cultured in rpmi 1640 medium containing 0.05, 0.1, 1 and 10 nm alpha-msh and the production of superoxide anion was measured via the reduction of nitroblue tetrazolium (nbt) in vitro. macrophages incubated with alpha-msh showed an increase in the production of superoxide anion in comparison to control macro ... | 2003 | 12543549 |
| molecular cloning and characterisation of a carp (cyprinus carpio) cytokine-like cdna that shares sequence similarity with il-6 subfamily cytokines cntf, osm and lif. | in the course of suppression subtractive hybridisation between sodium alginate-induced peritoneal cells (sa-pc) and normal head kidney cdnas in common carp (cyprinus carpio), a cytokine-like cdna clone was found. the clone, named m17, contains a 1600bp nucleotide sequence that encodes a 215 amino acid putative protein that would have a pi of 9.01 and would include a 33 amino acid signal peptide. the 3' untranslated region has seven attta mrna destabilising motifs that are common in cytokines and ... | 2003 | 12543126 |
| fiber connections of the torus longitudinalis in a teleost: cyprinus carpio re-examined. | fiber connections of the carp torus longitudinalis were re-examined by means of tract-tracing methods. the torus longitudinalis projected mainly to the stratum marginale of the optic tectum, area pretectalis, and corpus cerebelli. the stratum marginale was anterogradely labeled only by biocytin, but not by horseradish peroxidase. because the stratum is composed of extremely fine axons of the small toral neurons, this may be ascribed to different molecular weights of the tracers. the main afferen ... | 2003 | 12541319 |
| distribution and characteristics of d-amino acid and d-aspartate oxidases in fish tissues. | the distributions of d-amino acid oxidase (d-aao, ec 1.4.3.3) and d-aspartate oxidase (d-aspo, ec 1.4.3.1) activities were examined on several tissues of various fish species. both enzyme activities were commonly high in kidney and liver and low in intestine with some exceptions. after oral administration of d-alanine at 5 micromol /g body weight(-1)day(-1) to carp for 30 days, d-aao activity increased by about 8-, 3-, and 1.5-fold in intestine, hepatopancreas, and kidney, respectively, whereas ... | 2003 | 12541299 |
| an increased expression of nucleolin is associated with a physiological nucleolar segregation. | nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. we characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. we show that the carp cdna deduced amino acid sequence ex ... | 2003 | 12535655 |