Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| intracellular distribution of 73,000 and 72,000 dalton heat shock proteins in hela cells. | intracellular localization of 73,000 and 72,000 dalton heat shock proteins (hsp73/72) in hela cells that were heat shocked or treated with chemical stressors was investigated using indirect immunofluorescent staining. the antiserum used specifically recognized the hsp73/72 in hela cells, and hsps were increased by heating cells at 42 degrees c for 2 or 4 h and by prior treatment with chemical stressors (sodium arsenite, cadmium chloride, 8-hydroxyquinoline and ethanol). there was diffuse cytopla ... | 1986 | 3098874 |
| evaluation of a bacteriological screening method for drinking water control on board ships. | 2011 | 3095683 | |
| metastatic melanoma to the epididymis suspected on preoperative ultrasound. | 2010 | 3095399 | |
| purification and partial characterization of a vibrio hollisae hemolysin that relates to the thermostable direct hemolysin of vibrio parahaemolyticus. | hemolysin produced by vibrio hollisae (vh-rtdh), which is related to the thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus, was studied. vh-rtdh was purified by successive column chromatographies on diethylaminoethyl-cellulose and an immunoaffinity column coupled with anti vp-tdh immunoglobulin. the purified toxin was homogeneous, as demonstrated by conventional and sodium dodecyl sulfate--polyacrylamide gel electrophoresis (page). the molecular weight of vh-rtdh was slightly sma ... | 1986 | 3094925 |
| discordant effects of prednisone on anticardiolipin antibodies and the lupus anticoagulant. | 2011 | 3094545 | |
| effect of aldose reductase inhibitor (sorbinil) on integration of polyol pathway, pentose phosphate pathway, and glycolytic route in diabetic rat lens. | this study examines the effect of an aldose reductase inhibitor (sorbinil) on the flux of specifically labeled glucose through alternative pathways of metabolism in the lens of normal and diabetic rats 1 wk after the induction of diabetes with alloxan. in the diabetic rat lens, there was an apparent increase in the flux of glucose through the pentose phosphate pathway (ppp), as measured by the difference in the yields of 14co2 from [1-14c]glucose and [6-14c]glucose [c1-c6], this value was 0.087 ... | 1986 | 3093302 |
| ontogenetic development of pancreatic thyrotropin-releasing hormone in human foetuses and in infants. | the ontogeny of thyrotropin-releasing hormone (trh) in pancreata of human foetuses from 15-36 weeks of gestation and of infants has been studied. trh was detectable in the pancreas of a 15 week old foetus; a progressive increase of pancreatic trh content was observed until the 34th week of gestation, whereas a progressive decrease was found in the late period of pregnancy and in 1 year old infants. in contrast, the pancreatic insulin content showed a progressive increase during the entire pregna ... | 1986 | 3092527 |
| annual summary 1984. reported morbidity and mortality in the united states. | 2011 | 3092006 | |
| limitations in the use of 14c-glycocholate breath and stool bile acid determinations in patients with chronic diarrhea. | analysis of a modified 14c-glycocholate breath test on 165 consecutive in-patients being investigated for chronic diarrhea showed that the measurement of 14co2 between 3 and 6 h after oral dosing of 5 microci of 14c-glycocholic acid was of only limited use to distinguish between patients with crohn's disease (cd), idiopathic bile salt wastage (ibw), or ileal resection (ir) from those with the irritable bowel syndrome (ibs). continuing 14co2 collections for up to 24 h was of little more help in e ... | 2011 | 3090133 |
| concentrations of thioridazine and thioridazine metabolites in erythrocytes. | the concentrations of thioridazine and its main metabolites in erythrocytes from 61 thioridazine-treated patients were determined by gas-liquid chromatography. the mean and range of the erythrocyte concentrations, expressed as percentage of the corresponding plasma concentrations, were: thioridazine, 5.1% (2.0-10.6); side-chain sulfoxide, 5.6% (1.6-10.4); side-chain sulfone, 3.3% (1.1-6.8); ring sulfoxide 2.7% (0.8-4.9). the erythrocyte and plasma concentrations were significantly correlated. th ... | 2010 | 3088651 |
| effect of chemical sympathectomy with oxidopamine on systemic blood flow in rats with acute pancreatitis. | in response to previous demonstrations that either surgical or chemical sympathectomy can ameliorate the severity of acute experimental pancreatitis, and suggestions that the benefits of sympathectomy are related to alterations in the absolute blood flow or the pattern of blood flow within the pancreas or splanchnic organs, we studied blood flow in rats with acute deoxycholate pancreatitis. the animals had preliminary chemical sympathectomy with a false neurotransmitter (oxidopamine) prior to th ... | 1986 | 3087651 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| [enterotoxin-like factor(s) produced by vibrio parahaemolyticus]. | culture filtrates obtained from several isolates of vibrio parahaemolyticus were tested for their enterotoxin-like activities. also their antigenic relationship with cholera enterotoxin and kanagawa hemolysin were tested. in the chinese hamster ovary (cho) cells assay which are usually employed for detecting cholera toxin or heat labile enterotoxin produced by enterotoxigenic escherichia coli, crude culture filtrates obtained from both kanagawa positive and negative strains isolated from patient ... | 1986 | 3084368 |
| second-generation cephalosporins. | 1986 | 3081544 | |
| hematologic care of the surgical patient. | 1986 | 3081528 | |
| hematologic care of the surgical patient. | 1986 | 3081528 | |
| interaction of fibronectin-coated beads with attached and spread fibroblasts. binding, phagocytosis, and cytoskeletal reorganization. | after 15 min incubations, binding of 0.8-, 6-, and 16-microns fibronectin-coated latex beads occurred primarily at the margins of chick embryo fibroblasts that previously were attached and spread on fibronectin-coated glass coverslips. extensive phagocytosis of the smallest beads and some phagocytosis of the larger beads occurred within 2 h. following binding of the 16-micron beads, there were no changes in overall cell shape or in the distribution of several cytoskeletal proteins. there was, ho ... | 2011 | 3080317 |
| massive bladder hemorrhage. | 2010 | 3080196 | |
| [sarcoidosis, today]. | 2011 | 3079440 | |
| american clinical and climatological association. present members. | 2011 | 3077569 | |
| the nature of time: implications for research on aging. | contemporary research on aging gives little evidence of recognition of the underlying concepts of time. researchers have been much concerned with age as a variable but little with the explication of aging as a process. most authors do not define aging as they use the term or explain how time enters their causal explanations. in this article major concepts of time are reviewed and distinctions are made between physical, biological, psychological, and social time or age, which all might be classif ... | 2011 | 3076507 |
| the role of the ras oncogene in the formation of tumours. | a c-ha-ras 1 oncogene, cloned from the ej human bladder carcinoma cell line, was inserted into a shuttle vector carrying the selectable marker gene gpt that encodes the enzyme xanthine-guanine phosphoribosyl transferase. this construct, psv2gptej, was transfected into nih 3t3 cells by the calcium phosphate precipitation method and cells that had incorporated the plasmid were selected by growth in the presence of mycophenolic acid to which gpt confers resistance. a number of transfectant clones w ... | 1988 | 3075615 |
| the role of the ras oncogene in the formation of tumours. | a c-ha-ras 1 oncogene, cloned from the ej human bladder carcinoma cell line, was inserted into a shuttle vector carrying the selectable marker gene gpt that encodes the enzyme xanthine-guanine phosphoribosyl transferase. this construct, psv2gptej, was transfected into nih 3t3 cells by the calcium phosphate precipitation method and cells that had incorporated the plasmid were selected by growth in the presence of mycophenolic acid to which gpt confers resistance. a number of transfectant clones w ... | 1988 | 3075615 |
| [risks in the development of hypertension]. | 2010 | 3073295 | |
| diphtheria toxin entry: protein translocation in the reverse direction. | 2005 | 3072713 | |
| porous acrylic cement: evaluation of a novel implant material. | the preparation and some properties of in situ curing porous acrylic cement, a modification of the traditional bone cement, are described. through numerous animal experiments, the behavior of the porous cement in a biological environment has been studied. the histological evaluation reveals that hard and soft tissues readily grow into the pores of the cement, thus anchoring the implant to the host tissues. some inflammatory foci, decreasing in number with residence time, together with varying nu ... | 2010 | 3071237 |
| porous acrylic cement: evaluation of a novel implant material. | the preparation and some properties of in situ curing porous acrylic cement, a modification of the traditional bone cement, are described. through numerous animal experiments, the behavior of the porous cement in a biological environment has been studied. the histological evaluation reveals that hard and soft tissues readily grow into the pores of the cement, thus anchoring the implant to the host tissues. some inflammatory foci, decreasing in number with residence time, together with varying nu ... | 2010 | 3071237 |
| [peyronie's disease: an objective review]. | 2011 | 3070700 | |
| [academic dissertations of military pharmacists on the history of pharmacy]. | 2011 | 3067684 | |
| psychochemistry in psychiatry. papers presented in memory of ole j. rafaelsen. | 2011 | 3067535 | |
| [pharmacokinetics of non-steroidal anti-inflammatory agents in the elderly]. | 2011 | 3067421 | |
| [use of prostacyclin in patients with ischemic stroke. a double-blind method ii]. | thirty patients with acute ischaemic stroke were allocated randomly into a group treated with prostacyclin and a group receiving placebo. the treatment was started 24 to 72 hours after the onset of stroke. prostacyclin sodium (wellcome, u.k. or chinoin, hpr) or its solvent (glycine buffer) were administered intravenously once daily during 2 weeks in 6-hour infusions. prostacyclin was infused at rates of 2.5-5.0 ng/kg/min. statistically significant improvement appeared from the second day on in t ... | 2010 | 3067105 |
| [use of prostacyclin in patients with ischemic stroke. a double-blind method ii]. | thirty patients with acute ischaemic stroke were allocated randomly into a group treated with prostacyclin and a group receiving placebo. the treatment was started 24 to 72 hours after the onset of stroke. prostacyclin sodium (wellcome, u.k. or chinoin, hpr) or its solvent (glycine buffer) were administered intravenously once daily during 2 weeks in 6-hour infusions. prostacyclin was infused at rates of 2.5-5.0 ng/kg/min. statistically significant improvement appeared from the second day on in t ... | 2010 | 3067105 |
| emphasis on preventive perinatology: a suitable alternative for developing countries. | the potential efficacy of preventive interventions in latin america to reduce perinatal morbidity and mortality is reviewed. the most important perinatal risk factors associated with pregnancy and delivery are young age of the mother, low socioeconomic status, low education, malnutrition, lack of prenatal care, pregnancy-induced-hypertension, perinatal infections, alcohol, smoking, and iatrogenic causes. adequate data are lacking to determine the magnitude of these factors in latin america. iatr ... | 2011 | 3065947 |
| [phenylketonuria: current status of the problem]. | 2011 | 3064840 | |
| insulin controlled-release microcapsules to prolong the hypoglycemic effect in diabetic rats. | a solvent evaporation process was employed for preparing insulin microcapsules by using biodegradable polymers [polylactic acid (pla), hp-55] and non-biodegradable polymers [ethylcellulose (ec), ethylenevinyl acetate (eva)]. the release behavior of insulin microcapsules in ph 7.4 phosphate buffer solution was undertaken by a continuous flow column method. seven types of insulin microcapsules were respectively injected into the flanks of fasting-diabetic sd rats induced by streptozotocin. the glu ... | 1988 | 3064829 |
| [being a fan of delegated matters (aleksandr vasil'evich borisovskiĭ)]. | 2011 | 3064403 | |
| [absence of vibrio parahaemolyticus in raw fish]. | 1988 | 3064232 | |
| [results of screening for hearing disorders using the ewing test]. | late developments support that deaf children have an urgent need for rehabilitation and hence for early search for hearing defects. one of the modalities to achieve this goal is mass screening of populations. in belgium the long established well-baby-clinics provide a commodity for these programs. as most infants attend regularly these clinics, it was evident to perform here the testing procedure. the ewing-test was chosen as the tool for the screening. we started about 1980. in this discussion ... | 2009 | 3063060 |
| [echographic and radiographic aspects of a case of burkitt's lymphoma]. | 2011 | 3060909 | |
| a precision alignment frame for endosseous post dental implants. | the advantages of the precision alignment frame are that it (1) accurately positions and aligns the initial pilot drill at implant surgery, (2) has no adverse effects on the diagnostic cast, (3) eliminates the need for an incision or raising a flap when locating a submerged implant root for transmucosal connection, (4) consistently and accurately records the relation of the implant root to the radiograph, (5) permits long-term evaluation of the implant after restoration, (6) has components that ... | 2010 | 3058942 |
| a precision alignment frame for endosseous post dental implants. | the advantages of the precision alignment frame are that it (1) accurately positions and aligns the initial pilot drill at implant surgery, (2) has no adverse effects on the diagnostic cast, (3) eliminates the need for an incision or raising a flap when locating a submerged implant root for transmucosal connection, (4) consistently and accurately records the relation of the implant root to the radiograph, (5) permits long-term evaluation of the implant after restoration, (6) has components that ... | 2010 | 3058942 |
| nonionic contrast agents and coagulation. | the chemotoxicity of nonionic and ionic contrast media is discussed with reference to chemical structure. second-generation nonionic contrast media have the lowest chemotoxicity of any agents because of the uniform distribution of hydrophilic groups around the molecule, which mask the hydrophobic iodine atoms. previous investigations show that second-generation nonionic agents have the same qualitative hematologic effects as other contrast agents. the less marked quantitative hematologic effects ... | 2010 | 3058638 |
| current perspectives on the epidemiology and pathogenesis of clinically significant vibrio spp. | recent taxonomic advances have now implicated several different vibrio species as human pathogens. while the most common clinical presentation of vibrio infection continues to be gastroenteritis, an increasing number of extraintestinal infections are being reported, particularly in immunocompromised individuals. detection of vibrio infections requires a good clinical history and the use of appropriate isolation and identification procedures by the laboratory to confirm illnesses attributed to vi ... | 1988 | 3058295 |
| screening for silent myocardial ischemia. | since many patients do not experience angina prior to myocardial infarction or sudden death, screening of asymptomatic high-risk patients is necessary. exercise stress testing of asymptomatic patients with coronary risk factors can be performed by family physicians in their offices. for the physician trying to develop a rational screening policy for silent myocardial ischemia, the relatively low yield of positive test results creates a management dilemma. | 2010 | 3057838 |
| screening for silent myocardial ischemia. | since many patients do not experience angina prior to myocardial infarction or sudden death, screening of asymptomatic high-risk patients is necessary. exercise stress testing of asymptomatic patients with coronary risk factors can be performed by family physicians in their offices. for the physician trying to develop a rational screening policy for silent myocardial ischemia, the relatively low yield of positive test results creates a management dilemma. | 2010 | 3057838 |
| criteria for an increased specificity of mri interpretation in elderly subjects with suspected multiple sclerosis. | we reviewed the mris of 49 asymptomatic volunteers (age range, 31 to 77 years) and of 50 ms patients (age range, 14 to 63) for areas of increased signal (ais) and features discriminating ms lesions from lesions seen with normal aging. we obtained optimal specificity of mri interpretation (100%) if we required at least two of the following three ais features--size greater than or equal to 6 mm, abutting ventricular bodies, infratentorial location--for a positive mri diagnosis of ms. applying thes ... | 2011 | 3057397 |
| fabric of many-colored threads. | 2010 | 3056893 | |
| fabric of many-colored threads. | 2010 | 3056893 | |
| [malignant lymphomas of various histological types in pike from the eastern portion of the baltic sea]. | malignant lymphomas have been found in pikes (esox lucius l.) caught in the eastern part of the baltic sea. the frequency of the disease has increased in recent years. three different histological types of malignant lymphoma are described in 32 pikes: poorly differentiated diffuse lymphocytic lymphoma, mixed (histiocytoid-lymphocytic) diffuse lymphoma and undifferentiated (anisomorphic) diffuse lymphoma. | 2011 | 3053150 |
| racism, the national health service, and the health of black people. | racism has been and is central to an understanding of the health of black people in britain. black people have played and are playing a central role in the national health service (nhs). their role is, however, shaped by racism. their experiences as consumers of the nhs are also shaped by racism--in terms of their treatment for both physical and mental health problems. in addition, their specific health problems such as sickle cell anemia have not received the attention they deserve. the nhs has ... | 2011 | 3049409 |
| patient selection and results of cardiac transplantation in patients with cardiomyopathy. | cardiac transplantation for the treatment of end-stage congestive heart failure has been shown to be of benefit regardless of the etiology. with few exceptions, the evaluation of patients with end-stage heart failure is the same, regardless of the etiology. in those with cardiomyopathy not as a result of cad, special attention must be given to exclude secondary causes of cardiomyopathy such as amyloidosis, hemochromatosis, and sarcoidosis, as well as generalized systemic illnesses that may also ... | 2010 | 3048684 |
| patient selection and results of cardiac transplantation in patients with cardiomyopathy. | cardiac transplantation for the treatment of end-stage congestive heart failure has been shown to be of benefit regardless of the etiology. with few exceptions, the evaluation of patients with end-stage heart failure is the same, regardless of the etiology. in those with cardiomyopathy not as a result of cad, special attention must be given to exclude secondary causes of cardiomyopathy such as amyloidosis, hemochromatosis, and sarcoidosis, as well as generalized systemic illnesses that may also ... | 2010 | 3048684 |
| sensitivity of some marine bacteria, a moderate halophile, and escherichia coli to uncouplers at alkaline ph. | the inhibitory effects of uncouplers on amino acid transport into three marine bacteria, vibrio alginolyticus 118, vibrio parahaemolyticus 113, and alteromonas haloplanktis 214, into a moderate halophile, vibrio costicola nrc 37001, and into escherichia coli k-12 were found to vary depending upon the uncoupler tested, its concentration, and the ph. higher concentrations of all of the uncouplers were required to inhibit transport at ph 8.5 than at ph 7.0. the protonophore carbonyl cyanide m-chlor ... | 1988 | 3045092 |
| [x-ray computed tomography in venous pathology]. | after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed. | 2011 | 3043474 |
| transfusion of bone marrow red cells during bone marrow harvests. | large volumes of bone marrow may be required for certain types of autologous bone marrow transplants. the present study was done to determine whether red cells obtained during a bone marrow harvest would be useful in reducing homologous transfusion requirements. a group of patients receiving standard transfusion support during the harvest (group 1) was compared to a group that received processed bone marrow red cells (pbmrbc) (group 2). using the cobe 2991 cell processor, 90% of the harvested bo ... | 2011 | 3042443 |
| [splenic cysts in childhood. presentation of 4 cases and review of the literature]. | 2010 | 3041316 | |
| two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines. | the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ... | 1987 | 3038946 |
| phosphate regulation of gene expression in vibrio parahaemolyticus. | the synthesis of a major outer membrane protein, ompp, in vibrio parahaemolyticus was induced by growth in media deficient in phosphate. the gene, ompp, encoding this protein was cloned. synthesis of ompp in escherichia coli was regulated by the availability of phosphate, and this control required the function of pho regulatory genes of e. coli. analysis of gene fusion strains constructed by mutagenesis with transposon mini-mulux revealed that ompp was transcriptionally regulated in v. parahaemo ... | 1987 | 3038839 |
| peculiarities of the endocrine time structure in noninsulin-dependent adult-onset (type ii) diabetes mellitus. | twenty noninsulin-dependent elderly diabetic patients, ten of whom were treated by oral hypoglycemic agents and ten of whom were regulated by diet alone, and 20 clinically healthy subjects matched for age, sex, height, and weight were examined with six blood and six urine samples at 4-hr intervals over a 24-hr span. plasma acth, cortisol, aldosterone, and dehydroepiandrosterone-sulfate (dhea-s) were determined by radioimmunoassay (ria); epinephrine, norepinephrine, and dopamine in urine were det ... | 2010 | 3037559 |
| the hypothalamic-pituitary-adrenal axis in depression. | 2010 | 3035885 | |
| communicating leveled clinical expectations to nursing students. | nurse educators agree on the need for objectives in the classroom situation and frequently communicate them in a written form. written expectations for clinical, however, are rarely given to student nurses prior to their clinical experience. clinical expectations are understood by instructors, but communicating them to students result in mixed interpretations. the article describes the development of a written method of communicating clinical expectations to solve this problem. this method corre ... | 2010 | 3035139 |
| active lambda and kappa antibody gene rearrangement in abelson murine leukemia virus-transformed pre-b cell lines. | the two abelson murine leukemia virus (a-mulv)-transformed cell lines, bm18-4 and abc-1, undergo immunoglobulin l-chain gene recombination during passage in tissue culture. bm18-4 cells are capable of kappa gene recombination, whereas abc-1 cells are capable of both kappa and lambda gene recombination. the expression of h chains is apparently not necessary for continuing l chain gene recombination in either of these cells, although h-chain expression may have been involved in the initiation of l ... | 1987 | 3035056 |
| a novel mechanism for utilization of extracellular amp in vibrio parahaemolyticus. | vibrio parahaemolyticus could grow with amp, adp or atp as the sole source of carbon. in the presence of cl-, a membrane-bound cl(-)-dependent 5'-nucleotidase seemed to hydrolyze the nucleotides extracellularly, and then the cells took up the resulting adenosine. in the absence of cl-, although no significant dephosphorylation of the nucleotides occurred, the cells could still grow with amp, but not with adp or atp. moreover, in the presence of cl-, zn2+ inhibited the 5'-nucleotidase, and inhibi ... | 1987 | 3034248 |
| calmodulin in porcine malignant hyperpyrexia. | the anaesthetic complication malignant hyperpyrexia (mh) is due to an elevation of the myoplasmic ca2+ concentration. examination of calmodulin isolated from mh susceptible swine suggests that the disorder in calcium regulation in mh is not due to an abnormality in calmodulin. | 1987 | 3032704 |
| role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats. | permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ... | 1987 | 3032567 |
| membrane phospholipids alter nutrient transport and drug toxicity in tumorigenic fibroblasts. | the phospholipid polar head group composition of lm cell plasma membranes was nutritionally altered by choline analogue supplementation. phosphatidylcholine (pc), normally 60% of total phospholipid, was depleted by 60 to 90% in membranes from cells cultured with n, n'-dimethylethanolamine (dme), n-monomethylethanolamine (me), and ethanolamine (e). enrichment of lm cell membranes with choline analogues, such as dme-, me-, and e-containing phospholipids, decreased the transport of [3h]thymidine, [ ... | 2011 | 3032553 |
| pharmacological modification of pulmonary vascular injury: possible role of camp. | experiments were designed to test the hypothesis that drugs which increase adenosine 3',5'-cyclic monophosphate (camp) in the lung would prevent the pulmonary hypertension and the increase in vascular permeability caused by the infusion of the oxidant lipid peroxide, tert-butyl hydroperoxide (t-bu-ooh), in isolated rabbit lungs perfused with krebs-henseleit buffer. pretreatment with indomethacin or verapamil was also studied, since these drugs block the increase in pulmonary arterial pressure ca ... | 1987 | 3031002 |
| seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus). | the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ... | 1987 | 3030193 |
| seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus). | the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ... | 1987 | 3030193 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| isolation of the rhodopseudomonas sphaeroides form i ribulose 1,5-bisphosphate carboxylase/oxygenase large and small subunit genes and expression of the active hexadecameric enzyme in escherichia coli. | a library of cloned rhodopseudomonas sphaeroides dna was screened by colony hybridization for form i ribulose 1,5-bisphosphate carboxylase/oxygenase (rubpc/o) sequences using heterologous rubpc/o probes. a recombinant plasmid was identified that hybridized to both the anacystis nidulans and the r. sphaeroides form ii rubpc/o genes. subcloning of a hybridizing 4-kb smai fragment allowed expression of active enzyme in escherichia coli that was identical to form i rubpc/o based on polyacrylamide ge ... | 1986 | 3023189 |
| isolation of the rhodopseudomonas sphaeroides form i ribulose 1,5-bisphosphate carboxylase/oxygenase large and small subunit genes and expression of the active hexadecameric enzyme in escherichia coli. | a library of cloned rhodopseudomonas sphaeroides dna was screened by colony hybridization for form i ribulose 1,5-bisphosphate carboxylase/oxygenase (rubpc/o) sequences using heterologous rubpc/o probes. a recombinant plasmid was identified that hybridized to both the anacystis nidulans and the r. sphaeroides form ii rubpc/o genes. subcloning of a hybridizing 4-kb smai fragment allowed expression of active enzyme in escherichia coli that was identical to form i rubpc/o based on polyacrylamide ge ... | 1986 | 3023189 |
| identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol. | although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ... | 1986 | 3022716 |
| thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus. | the acyclovir resistant mutant of varicella-zoster virus acv-r (a 8) induced the same level of thymidine kinase activity in infected cells as the parent kawaguchi strain. however, it induced less deoxycytidine kinase activity and did not induce phosphorylating activity for the nucleotide analogue, 9-(2 hydroxy-ethoxymethyl)-guanine-(acyclovir). another acyclovir resistant mutant, acv-r (a 4), which is cross-resistant to phosphonoacetate and is thought to be a viral dna polymerase mutant, induced ... | 1986 | 3022711 |
| spermine antagonises the effects of dexamethasone, glucagon and cyclic amp in increasing the activity of phosphatidate phosphohydrolase in isolated rat hepatocytes. | rat hepatocytes were incubated in monolayer culture, under serum free conditions, for 8 h. glucagon (10 nm), 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (100 microm) and dexamethasone (100 nm) increased the activity of phosphatidate phosphohydrolase by approx. 2-, 3.6- and 3.3-fold, respectively. spermine alone had no significant effect. spermine (2.5 mm) almost completely inhibited the glucagon induced increase in phosphohydrolase activity. it only partially inhibited the dexamet ... | 1986 | 3021529 |
| eicosapentaenoic acid inhibits synthesis and secretion of triacylglycerols by cultured rat hepatocytes. | primary cultures of rat hepatocytes were used to study the effects of eicosapentaenoic and oleic acid on synthesis and secretion of triacylglycerols associated with very low density lipoproteins. from the experiments the following was observed. oleic acid markedly stimulates secretion as well as synthesis of triacylglycerols, whereas eicosapentaenoic acid causes very little or no increase in secretion or synthesis as compared to a fatty-acid-free medium. the effects could already be observed aft ... | 1986 | 3021226 |
| eicosapentaenoic acid inhibits synthesis and secretion of triacylglycerols by cultured rat hepatocytes. | primary cultures of rat hepatocytes were used to study the effects of eicosapentaenoic and oleic acid on synthesis and secretion of triacylglycerols associated with very low density lipoproteins. from the experiments the following was observed. oleic acid markedly stimulates secretion as well as synthesis of triacylglycerols, whereas eicosapentaenoic acid causes very little or no increase in secretion or synthesis as compared to a fatty-acid-free medium. the effects could already be observed aft ... | 1986 | 3021226 |
| [effects of 5 derivatives of 3-azabicyclo [3,3,1]nonanes on isolated guinea pig ileum myenteric plexus-longitudinal muscle]. | 1985 | 3020868 | |
| response of young pigs to foot-and-mouth disease oil emulsion vaccination in the presence and absence of maternally derived neutralising antibodies. | serum samples and bodyweights were taken at regular intervals for six to seven months from piglets, born to foot-and-mouth disease (fmd) vaccinated or unvaccinated sows, which had been vaccinated at one, two, four or eight weeks old. young pigs, devoid of maternally derived antibodies (mda), were capable of responding to fmd vaccination at one week old, with no deleterious effects on their growth rate. however, their immunity to experimental infection at six to seven months old was poor (33.3 pe ... | 1986 | 3020657 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes. | two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ... | 1986 | 3020551 |
| induction of an unusual type of shared phosphorylation in human and avian cells by tumor-promoting phorbol esters or transformation. | alterations in patterns of protein phosphorylation after exposure to phorbol esters were compared in chicken embryo fibroblasts and kd cells (a human fibroblast line) by two-dimensional gel electrophoresis. a substantial increase in phosphorylation was observed of a major, markedly acidic protein of pi = 4.5 in two-dimensional gels of each cell type. however, the apparent molecular weights of these phosphoproteins differed substantially in the two species with a molecular weight of 67,000 in chi ... | 1986 | 3019525 |
| [experimental nephritis induced by coxsackie b4 virus in mice--transient mesangial proliferation associated with acute viremia]. | 1986 | 3018334 | |
| the adenosine triphosphate-pyrophosphate isotopic exchange reaction: a tool for determination of tryptophan. | quantitative determination of tryptophan at the picomole level is described, using the atp-[32p]ppi isotopic exchange reaction catalyzed by tryptophanyl-trna synthetase. sensitivity limits of 500 fmol were obtained. the presence of other amino acids at a 1000-fold excess over tryptophan did not interfere significantly with the quantitative determination of tryptophan. the specificity of the reaction was checked using five tryptophan analogs. these analogs did not prevent the determination of try ... | 2010 | 3014918 |
| regulation of lateral flagella gene transcription in vibrio parahaemolyticus. | two distinctly different organelles of locomotion are produced by vibrio parahaemolyticus. the polar flagellum is responsible for motility in a liquid environment (swimming), and the lateral flagella enable the bacteria to move over surfaces (swarming). synthesis of lateral flagella occurs when v. parahaemolyticus is grown on agar media but not when it is grown in liquid media. we used lux (luminescence gene) fusions to conveniently and sensitively analyze the factors which influence transcripti ... | 1986 | 3013835 |
| transposable resistance to trimethoprim and 0/129 in vibrio cholerae. | vibrio cholerae biotype el tor strain bm2508, resistant to trimethoprim, 0/129, streptomycin and spectinomycin was isolated from the faeces of a child with severe diarrhoea. resistance to trimethoprim and 0/129 was due to a dihydrofolate reductase type i and resistance to streptomycin-spectinomycin to a 3'',9-aminoglycoside-aminocyclitol adenylyltransferase. the resistance genes were not transferable to escherichia coli and, as inferred from ultracentrifugation in cesium chloride-ethidium bromid ... | 1986 | 3013828 |
| a novel type of human papillomavirus associated with genital neoplasias. | the role of human papillomaviruses (hpvs) in the development of genital neoplasias has been well documented. the genomes of two hpv types, hpv16 and hpv18, have been found to be associated with about 70% of invasive carcinomas of the uterine cervix. as, under non-stringent hybridization conditions, hpv dna sequences have been detected in about 90% of cervical carcinomas, it seems likely that additional hpv types are associated with these tumours. here we report the molecular cloning and characte ... | 1986 | 3012352 |
| cloning and expression of the exfoliative toxin b gene from staphylococcus aureus. | exfoliative toxin type b is produced by bacteriophage group ii strains of staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. in addition to exfoliative toxin b, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. these phenotypes, as well as resistance to cadmium, were lost after elimination of a 37.5-kilobase plasmid, prw001, from s. aureus ut0007. transduction and transformation showed that prw001 carries the structural ... | 1986 | 3009410 |
| densities and sizes of the main masticatory muscles in computed tomography compared with clinical findings related to temporomandibular joint (tmj) dysfunction. | computed tomography (ct) has a good resolution capacity and is an excellent method for measuring tissue densities. the aim here was to compare the densities and sizes of the main masticatory muscles, the masseter, medial pterygoid and lateral pterygoid muscles, as obtained with ct, with clinical findings in patients with tmj dysfunction (25) and controls (29). the densities of the masseter muscles and the medial pterygoid muscles showed no statistical difference between the patients and controls ... | 2010 | 3008236 |
| effect of the hyp mutation and diet-induced hyperparathyroidism on renal parathyroid hormone- and forskolin-stimulated adenosine 3',5'-monophosphate production and brush border membrane phosphate transport. | the present study was undertaken to investigate the effect of the hyp mutation and diet-induced hyperparathyroidism on renal responsiveness to pth and forskolin and to determine whether the renal brush border membrane phosphate transport defect is expressed in the vitamin d- and calcium-deficient hyp mouse. our results indicate that pth is a potent activator of camp synthesis in renal slices obtained from vitamin d-replete normal mice and hyp littermates. however, in the mutants, the amount of c ... | 1986 | 3004890 |
| nucleotide sequence and in vivo expression of the ilvy and ilvc genes in escherichia coli k12. transcription from divergent overlapping promoters. | the ilvc gene of escherichia coli k12 encodes acetohydroxy acid isomeroreductase, the second enzyme in the parallel isoleucine-valine biosynthetic pathway. previous data have shown that transcription of the ilvc gene is induced by the acetohydroxy acid isomeroreductase substrates, acetohydroxybutyrate or acetolactate, and that this substrate induction of ilvc expression is mediated by a positive activator encoded by the ilvy gene. we report here the isolation and complete nucleotide sequence of ... | 1986 | 3003115 |
| malignant mixed mullerian tumor arising in extragenital endometriosis: report of a case and review of the literature. | a primary malignant mixed mullerian tumor (mmmt) was found in the wall of the rectum of a 67-year-old female. the uterus, fallopian tubes, and ovaries had been previously excised for benign disease. the tumor was associated with foci of benign endometriosis. a literature review of extragenital mmmts indicates that these are extremely rare tumors. this case supports the theory that endometriosis may serve as a progenitor to extraendometrial malignancy. | 2010 | 3002920 |
| serum angiotensin-converting enzyme levels in sarcoid arthritis. | the serum angiotensin-converting enzyme (sace) level is elevated in 75% of patients with sarcoidosis and often is associated with disease activity. in an attempt to correlate the sace level with sarcoid arthritis, we did a retrospective chart review of 116 patients with sarcoidosis. of the 24 patients who complained of arthritis, five were excluded from the study because they were receiving corticosteroids, sace levels were not determined, or another cause for their arthritis was found. the mean ... | 2010 | 3002290 |
| purification of a metalloproteinase inhibitor from human rheumatoid synovial fluid. | a metalloproteinase inhibitor present in human rheumatoid synovial fluid was purified by a combination of heparin-sepharose chromatography, concanavalin a-sepharose chromatography, ion-exchange chromatography and gel filtration. the mr of the purified inhibitor was 28000 by sds/polyacrylamide-gel electrophoresis and 30000 by gel filtration. the inhibitor blocked the activity of the metalloproteinases collagenase, gelatinase and proteoglycanase, but not thermolysin or bacterial collagenase. the s ... | 1985 | 3000352 |
| effect of pyrimidinone treatment on lethal and immunosuppressive murine cytomegalovirus infection. | the 2-amino-5-halo-pyrimidinones, which are potent interferon inducers and antiviral agents, were found to be protective against lethal cytomegalovirus (cmv) challenge in weanling or neonatal mice when administered before virus challenge. this protection was dependent upon the dosage of pyrimidinone administered. weanling mice infected with a sublethal challenge of cmv exhibited moderate to severe immunosuppression as measured by reduced splenic cell blastogenic responses in vitro to the mitogen ... | 1985 | 3000290 |
| [comparative efficacy of disinfectants on the poliovirus]. | 2010 | 2999938 | |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |