Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| timing of referral for lung transplantation for cystic fibrosis: overemphasis on fev1 may adversely affect overall survival. | (1) report our experience with referral for lung transplantation. (2) review survival in cvstic fibrosis (cf) patients without lung transplantation after fev1 remains < 30% predicted for 1 years. | 1999 | 10084492 |
| all in the family: structural and evolutionary relationships among three modular proteins with diverse functions and variable assembly. | the crystal structures of three proteins of diverse function and low sequence similarity were analyzed to evaluate structural and evolutionary relationships. the proteins include a bacterial bleomycin resistance protein, a bacterial extradiol dioxygenase, and human glyoxalase i. structural comparisons, as well as phylogenetic analyses, strongly indicate that the modern family of proteins represented by these structures arose through a rich evolutionary history that includes multiple gene duplica ... | 1998 | 10082363 |
| melioidosis. | melioidosis is an infection with the gram-negative bacillus burkholdria pseudomallei, previous known as pseudomonas pseudomallei. we present a case report of a man with non-hodgkin's lymphoma, who after visiting indonesia presented himself with a urosepsis, with positive cultures to b. pseudomallei. melioidosis is endemic in areas of southeast asia and the northern part of australia. infection with the b. pseudomallei has a high mortality rate. diagnosis can be made on the basis of cultures, the ... | 1999 | 10079683 |
| identification and characterization of is2404 and is2606: two distinct repeated sequences for detection of mycobacterium ulcerans by pcr. | molecular analysis of mycobacterium ulcerans has revealed two new insertion sequences (iss), is2404 and is2606. is2404 was identified by complete sequencing of a previously described repetitive dna segment from m. ulcerans. this element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame (orf) potentially encoding a protein of 327 amino acids (aa), and apparently generates 7-bp direct repeats upon transposition. amino acid similarity was found between the putative t ... | 1999 | 10074520 |
| comparison of isolation media for recovery of burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. | burkholderia cepacia selective agar (bcsa) has previously been devised for isolation of b. cepacia from respiratory secretions of patients with cystic fibrosis and tested under research laboratory conditions. here we describe a study in which bcsa, oxidation-fermentation polymyxin bacitracin lactose agar (ofpbl), and pseudomonas cepacia agar (pca) were compared in routine culture procedures for the ability to grow b. cepacia and inhibit other organisms. three hundred twenty-eight specimens from ... | 1999 | 10074517 |
| effect of urokinase on the extracellular virulence properties of pseudomonas aeruginosa and burkholderia cepacia. | the influence of urokinase and oxygen availability on growth, siderophore, protease and lipase production in burkholderia cepacia and non-mucoid (pa01) and mucoid (pawh) strains of pseudomonas aeruginosa was assessed for cells grown in batch culture under iron-restriction. siderophore production decreased with increasing concentration of urokinase in b. cepacia independent of oxygen availability but decreased in both strains of p. aeruginosa only under oxygen-depleted conditions. protease activi ... | 1999 | 10071863 |
| a nonhemolytic phospholipase c from burkholderia cepacia. | burkholderia cepacia is an opportunistic pathogen that causes serious pulmonary infections in cystic fibrosis patients. although several potential virulence factors-a protease, lipase, and two phospholipases c (one hemolytic and one nonhemolytic)-have been identified, only two, the protease and the lipase, have been described in detail. the goal of this study was to purify and characterize a nonhemolytic phospholipase c secreted by b. cepacia strain pc224c. the enzyme was concentrated from cultu ... | 1999 | 10069860 |
| lack of throat colonization with burkholderia cepacia among cystic fibrosis healthcare workers. | 1999 | 10064207 | |
| efflux-mediated aminoglycoside and macrolide resistance in burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to a wide range of antimicrobial agents including beta-lactams, aminoglycosides, macrolides, and polymyxins. we used tn5-ot182 to mutagenize b. pseudomallei to identify the genes involved in aminoglycoside resistance. we report here on the identification of amrab-opra, a multidrug efflux system in b. pseudomallei which is specific for both aminoglycoside and macrolide antibiotics. we isolated two transposon ... | 1999 | 10049252 |
| burkholderia cepacia infections in cystic fibrosis. | 1999 | 10048690 | |
| burkholderia cocovenenans (van damme et al. 1960) gillis et al. 1995 and burkholderia vandii urakami et al. 1994 are junior synonyms of burkholderia gladioli (severini 1913) yabuuchi et al. 1993 and burkholderia plantarii (azegami et al. 1987) urakami et al. 1994, respectively. | reference strains of burkholderia cocovenenans and burkholderia vandii were compared with strains of other burkholderia species using sds-page of whole-cell proteins, dna-dna hybridization and extensive biochemical characterization. burkholderia gladioli and b. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. burkholderia plantarii and b. vandii had indistinguishable whole-cell protein patterns but the b. vandii type strain differed from b. plantarii strains in ... | 1999 | 10028245 |
| lipopolysaccharide (lps) from burkholderia cepacia is more active than lps from pseudomonas aeruginosa and stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes. | whole cells and lipopolysaccharides (lpss) extracted from burkholderia cepacia, pseudomonas aeruginosa, stenotrophomonas maltophilia, and escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (tnf-alpha) from the human monocyte cell line monomac-6. b. cepacia lps, on a weight-for-weight basis, was found to have tnf-alpha-inducing activity similar to that of lps from e. coli, which was approximately four- and eightfold greater than the activity of lpss from p. a ... | 1999 | 10024601 |
| the type ii o-antigenic polysaccharide moiety of burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence. | melioidosis, an infection caused by the gram-negative bacterial pathogen burkholderia pseudomallei, is endemic in south-east asia and northern australia. acute septicaemic melioidosis is a major cause of morbidity and mortality, especially in north-east thailand. b. pseudomallei is highly resistant to the bactericidal activity of normal human serum (nhs), and we have found that b. pseudomallei 1026b multiplies in 10-30% nhs. we developed a simple screen for the identification of serum-sensitive ... | 1998 | 9988483 |
| attachment of burkholderia pseudomallei to pharyngeal epithelial cells: a highly pathogenic bacteria with low attachment ability. | respiratory infections are initiated by the attachment of bacteria to pharyngeal epithelial cells. we studied the attachment of burkholderia pseudomallei to pharyngeal epithelial cells. after one, two, three, and four washes, there were 22.6+/-8.9, 15.7+/-7.0, 6.8+/-3.1, and 4.6+/-1.1 (mean+/-sd) attached bacteria/cell, respectively. if the bacterial concentration was maintained at 1 x 10(8) colony-forming units (cfu)/ml and three washes were done, at concentrations of 2.5 x 10(4), 5 x 10(4), an ... | 1999 | 9988329 |
| [isolation and primary characteristics of a pseudomonas (burkholderia) pseudomallei plasmid]. | plasmid screening of reference pseudomonas pseudomallei strains isolated from patients and animals revealed cryptic plasmids with different molecular weights in 30 strains. plasmids were investigated by restriction analysis and dna-dna hybridization. cryptic plasmids were denoted as ppm1, pcm2, pcm3, and pcm4. the presence of plasmids in melioidosis agent permits their use for intraspecies typing of strains and for genetic studies. | 1998 | 9987750 |
| impact of microbiology practice on cumulative prevalence of respiratory tract bacteria in patients with cystic fibrosis. | investigators participating in the epidemiologic study of cystic fibrosis project began to collect microbiological, pulmonary, and nutritional data on cystic fibrosis (cf) patients at 180 north american sites in 1994. part of this study was a survey undertaken in august 1995 to determine microbiology laboratory practices with regard to pulmonary specimens from cf patients. the survey included a section on test ordering, completed by a site clinician, and a section on test performance and reporti ... | 1999 | 9986845 |
| purification and characterization of tert-butyl ester-hydrolyzing lipase from burkholderia sp. yy62. | an intracellular novel lipase which can hydrolyze t-butyl octanoate (tbo) was purified to homogeneity from crude cell-free extracts of burkholderia (formerly pseudomonas) sp. yy62 with 9% overall yield. seventy-four-fold purification was achieved by ammonium-sulfate precipitation, three consecutive open-column chromatographies (deae anion-exchange, sepharose cl-6b gel-filtration, and the second deae anion-exchange columns), and two hplcs (tsk g2000swxl gel-filtration and phenyl 5pw hydrophobic i ... | 1998 | 9972257 |
| in vitro activity of meropenem against common pathogenic bacteria isolated in taiwan. | the in vitro antimicrobial activity of meropenem, in comparison with nine other antimicrobial agents, against 12 different common pathogenic bacteria were evaluated to know the susceptibility of common bacteria to meropenem in taiwan. meropenem was active against most gram-positive, gram-negative, and anaerobic bacteria, including methicillin-sensitive staphylococcus aureus, streptococcus pyogenes, enterococcus faecalis, escherichia coli, klebsiella pneumoniae, enterobacter cloacae, pseudomonas ... | 1998 | 9934544 |
| from the centers for disease control and prevention. nosocomial burkholderia cepacia infection and colonization associated with intrinsically contaminated mouthwash--arizona, 1998. | 1999 | 9929074 | |
| new assay for rhizobitoxine based on inhibition of 1-aminocyclopropane-1-carboxylate synthase. | rhizobitoxine is synthesized by the legume symbiont bradyrhizobium elkanii and the plant pathogen burkholderia andropogonis. rhizobitoxine competitively inhibited 1-aminocyclopropane-1-carboxylate (acc) synthase ble-acs2 from the tomato, a key enzyme in the pathway of ethylene biosynthesis. based on this inhibition of acc synthase, we have developed a new assay for rhizobitoxine. | 1999 | 9925628 |
| high-level formation of active pseudomonas cepacia lipase after heterologous expression of the encoding gene and its modified chaperone in escherichia coli and rapid in vitro refolding. | the lipase from pseudomonas cepacia atcc 21808 (recently reclassified as burkholderia cepacia) is widely used by organic chemists for enantioselective synthesis and is manufactured from recombinant p. cepacia harboring on a plasmid the clustered genes for lipase and its chaperone. high levels of expression of inactive lipase (40%) in escherichia coli were achieved with pcytexp1 under the control of the strong, temperature-inducible lambdaprl promoter. however, no overexpression of the lipase cha ... | 1999 | 9925617 |
| alterations in adhesion, transport, and membrane characteristics in an adhesion-deficient pseudomonad. | a stable adhesion-deficient mutant of burkholderia cepacia g4, a soil pseudomonad, was selected in a sand column assay. this mutant (env435) was compared to the wild-type strain by examining the adhesion of the organisms to silica sand and their transport through two aquifer sediments that differed in their sand, silt, and clay contents. we compared the longitudinal transport of the wild type and the adhesion mutant to the transport of a conservative chloride tracer in 25-cm-long glass columns. ... | 1999 | 9925613 |
| inactivation of toluene 2-monooxygenase in burkholderia cepacia g4 by alkynes. | high concentrations of acetylene (10 to 50% [vol/vol] gas phase) were required to inhibit the growth of burkholderia cepacia g4 on toluene, while 1% (vol/vol) (gas phase) propyne or 1-butyne completely inhibited growth. low concentrations of longer-chain alkynes (c5 to c10) were also effective inhibitors of toluene-dependent growth, and 2- and 3-alkynes were more potent inhibitors than their 1-alkyne counterparts. exposure of toluene-grown b. cepacia g4 to alkynes resulted in the irreversible lo ... | 1999 | 9925593 |
| comparative in vitro activities of meropenem, imipenem, temocillin, piperacillin, and ceftazidime in combination with tobramycin, rifampin, or ciprofloxacin against burkholderia cepacia isolates from patients with cystic fibrosis. | we evaluated the activities of meropenem, imipenem, temocillin, piperacillin, and ceftazidime by determination of the mics for 66 genotypically characterized burkholderia cepacia isolates obtained from the sputum of cystic fibrosis patients. in vitro synergy assays, as performed by the time-kill methodology, of two- and three-drug combinations of the beta-lactams with tobramycin, rifampin, and/or ciprofloxacin were also performed with 10 strains susceptible, intermediate, or resistant to fluoroq ... | 1999 | 9925508 |
| neonatal meningitis and septicaemia caused by burkholderia pseudomallei. | we report a case of meningitis and one of fatal septicaemia in neonates due to burkholderia pseudomallei and review the literature on neonatal melioidosis. pneumonia was the primary presentation and was complicated by shock in the latter case. the epidemiological findings suggest that the cases reported from malaysia were community-acquired in contrast with those from the usa and thailand. | 1998 | 9924578 |
| biochemical and genetic evidence for meta-ring cleavage of 2,4, 5-trihydroxytoluene in burkholderia sp. strain dnt. | 2,4,5-trihydroxytoluene (tht) oxygenase from burkholderia sp. strain dnt catalyzes the conversion of tht to an unstable ring fission product. biochemical and genetic studies of tht oxygenase were undertaken to elucidate the mechanism of the ring fission reaction. the tht oxygenase gene (dntd) was previously localized to the 1.2-kb dna insert subcloned in the recombinant plasmid designated pjs76 (w. c. suen and j. c. spain, j. bacteriol. 175:1831-1837, 1993). analysis of the deduced amino acid se ... | 1999 | 9922262 |
| quorum sensing in burkholderia cepacia: identification of the luxri homologs cepri. | burkholderia cepacia has emerged as an important pathogen in patients with cystic fibrosis. many gram-negative pathogens regulate the production of extracellular virulence factors by a cell density-dependent mechanism termed quorum sensing, which involves production of diffusible n-acylated homoserine lactone signal molecules, called autoinducers. transposon insertion mutants of b. cepacia k56-2 which hyperproduced siderophores on chrome azurol s agar were identified. one mutant, k56-r2, contain ... | 1999 | 9922236 |
| a melanin pigment purified from an epidemic strain of burkholderia cepacia attenuates monocyte respiratory burst activity by scavenging superoxide anion. | the acquisition of burkholderia cepacia in some cystic fibrosis patients is associated with symptoms of acute pulmonary inflammation that may be life threatening. the ability of lipopolysaccharide (lps) from b. cepacia to prime a monocyte cell line for enhanced superoxide anion generation was investigated and compared with the priming activities of lpss from pseudomonas aeruginosa, stenotrophomonas maltophilia, and escherichia coli. the human monocyte cell line monomac-6 (mm6) was primed overnig ... | 1999 | 9916107 |
| antigenic differences between clinical and environmental isolates of burkholderia pseudomallei. | burkholderia pseudomallei is a free-living organism that causes the potentially lethal tropical infection melioidosis. the disease is endemic in many parts of eastern asia and northern australia. the presence of two distinct biotypes in soil can be reliably distinguished by their ability to assimilate l-arabinose. whereas some soil isolates could utilize this substrate (ara+), the remaining soil isolates and all clinical isolates tested so far could not (ara-). only the ara- isolates were virule ... | 1998 | 9886145 |
| burkholderia pseudomallei: the unbeatable foe? | although melioidosis has been recognized in thailand for many years and considerable progress in term of diagnosis and treatment was achieved, b. pseudomallei is still "the unbeatable foe", for several reasons as outlined here: under-recognition, high case-fatality rate, unacceptable relapse rate and a "time-bomb" for sero-positive patients. melioidosis is largely restricted to certain geographical areas. in thailand, it had long been considered a rare disease in thailand until ten cases with cu ... | 1998 | 9886137 |
| the phn genes of burkholderia sp. strain rp007 constitute a divergent gene cluster for polycyclic aromatic hydrocarbon catabolism. | cloning and molecular ecological studies have underestimated the diversity of polycyclic aromatic hydrocarbon (pah) catabolic genes by emphasizing classical nah-like (nah, ndo, pah, and dox) sequences. here we report the description of a divergent set of pah catabolic genes, the phn genes, which although isofunctional to the classical nah-like genes, show very low homology. this phn locus, which contains nine open reading frames (orfs), was isolated on an 11.5-kb hindiii fragment from phenanthre ... | 1999 | 9882667 |
| flagellin gene variation between clinical and environmental isolates of burkholderia pseudomallei contrasts with the invariance among clinical isolates. | the flagellin gene sequence from a clinical isolate of burkholderia pseudomallei was used to design oligonucleotide primers for pcr/rflp analysis of flagellin gene variation among clinical and environmental isolates of b. pseudomallei. genes from four clinical and six environmental isolates were amplified and compared by rflp. the clinical isolates were indistinguishable, but variation was detected among some of the environmental isolates. sequence analysis of flagellin gene amplified products d ... | 1998 | 9877189 |
| structural characterisation of a rhamnan and a fucorhamnan, both present in the lipopolysaccharide of burkholderia vietnamiensis strain lmg 10926. | the polymeric fraction isolated after mild acid hydrolysis of the lipopolysaccharide (lps) from burkholderia vietnamiensis strain lmg 10926 contained l-rhamnose (rha) and d-fucose (fuc). from nmr studies supported by the results of methylation analysis and smith degradation, it could be inferred that the material was probably a mixture of two glycans. one component was a linear rhamnan with a trisaccharide repeating unit (1); the other was a branched fucorhamnan with a tetrasaccharide repeating ... | 1998 | 9874236 |
| polycyclic aromatic hydrocarbon degradation by a new marine bacterium, neptunomonas naphthovorans gen. nov., sp. nov. | two strains of bacteria were isolated from creosote-contaminated puget sound sediment based on their ability to utilize naphthalene as a sole carbon and energy source. when incubated with a polycyclic aromatic hydrocarbon (pah) compound in artificial seawater, each strain also degraded 2-methylnaphthalene and 1-methylnaphthalene; in addition, one strain, nag-2n-113, degraded 2,6-dimethylnaphthalene and phenanthrene. acenaphthene was not degraded when it was used as a sole carbon source but was d ... | 1999 | 9872786 |
| in situ, real-time catabolic gene expression: extraction and characterization of naphthalene dioxygenase mrna transcripts from groundwater. | we developed procedures for isolating and characterizing in situ-transcribed mrna from groundwater microorganisms catabolizing naphthalene at a coal tar waste-contaminated site. groundwater was pumped through 0.22-microm-pore-size filters, which were then frozen in dry ice-ethanol. rna was extracted from the frozen filters by boiling sodium dodecyl sulfate lysis and acidic phenol-chloroform extraction. transcript characterization was performed with a series of pcr primers designed to amplify nah ... | 1999 | 9872763 |
| small genes/gene-products in escherichia coli k-12. | forty-two protein spots of observed m(r) 6-15 kda were resolved by two-dimensional gel electrophoresis, stained by coomassie blue and subjected to edman microsequencing. all of the proteins could be related back to their encoding open reading frames, thereby vindicating the bioinformatic tools currently utilised in their identification. however, only 14/42 gene-products were expressed as annotated. translation was confirmed for 14 open reading frames with no attributed function (ecogene y-entrie ... | 1998 | 9868784 |
| genetic and biochemical analyses of the tec operon suggest a route for evolution of chlorobenzene degradation genes. | the teca broad-spectrum chlorobenzene dioxygenase of burkholderia sp. strain ps12 catalyzes the first step in the mineralization of 1,2,4, 5-tetrachlorobenzene. the catabolic genes were localized on a small plasmid that belongs to the incpbeta incompatibility group. pcr analysis of the genetic environment of the tec genes indicated high similarity to the transposon-organized catabolic tcb chlorobenzene degradation genes of pseudomonas sp. strain p51. sequence analysis of the regions flanking the ... | 1999 | 9864349 |
| isolation and characterisation of disodium (4-amino-4-deoxy-beta-l- arabinopyranosyl)-(1-->8)-(d-glycero-alpha-d-talo-oct-2-ulopyranosylona te)- (2-->4)-(methyl 3-deoxy-d-manno-oct-2-ulopyranosid)onate from the lipopolysaccharide of burkholderia cepacia. | a trisaccharide was isolated from the core oligosaccharide in the lipopolysaccharide (lps) of burkholderia cepacia gifu 645 (atcc 25416, type strain) by methanolysis followed by hplc and saponification. it was identified by ms, methylation analysis and 1h and 13c nmr spectroscopy as disodium (4-amino-4-deoxy-beta-l-arabinopyranosyl)-(1-->8)-(d-glycero- alpha-d-talo-oct-2-ulopyranosylonate)-(2-->4)-(methyl 3-deoxy-d-manno-oct-2-ulopyranosid)onate. in addition to the trisaccharide derivative, meth ... | 1998 | 9861699 |
| molecular basis for the stabilization and inhibition of 2, 3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol. | the steady-state cleavage of catechols by 2,3-dihydroxybiphenyl 1, 2-dioxygenase (dhbd), the extradiol dioxygenase of the biphenyl biodegradation pathway, was investigated using a highly active, anaerobically purified preparation of enzyme. the kinetic data obtained using 2,3-dihydroxybiphenyl (dhb) fit a compulsory order ternary complex mechanism in which substrate inhibition occurs. the km for dioxygen was 1280 +/- 70 microm, which is at least 2 orders of magnitude higher than that reported fo ... | 1998 | 9857017 |
| rapid identification of burkholderia pseudomallei by latex agglutination based on an exopolysaccharide-specific monoclonal antibody. | we recently identified a constitutively expressed exopolysaccharide of burkholderia pseudomallei which is composed of a unique linear tetrasaccharide repeating unit consisting of three galactose residues and one 3-deoxy-d-manno-2-octulosonic acid residue. in this study we developed a latex agglutination test based on monoclonal antibody 3015, which is specific for this exopolysaccharide, and evaluated this test for rapid identification of b. pseudomallei grown on agar plates. all 74 environmenta ... | 1999 | 9854098 |
| novel organization of the genes for phthalate degradation from burkholderia cepacia dbo1. | burkholderia cepacia dbo1 is able to utilize phthalate as the sole source of carbon and energy for growth. two overlapping cosmid clones containing the genes for phthalate degradation were isolated from this strain. subcloning and activity analysis localized the genes for phthalate degradation to two separate regions on the cosmid clones. analysis of the nucleotide sequence of these two regions showed that the genes for phthalate degradation are arranged in at least three transcriptional units. ... | 1998 | 9851995 |
| dry-season outbreak of melioidosis in western australia. | 1998 | 9843113 | |
| species-specific oligonucleotides for enumeration of pseudomonas putida f1, burkholderia sp. strain js150, and bacillus subtilis atcc 7003 in biodegradation experiments. | species-specific sequences were identified within the v4 variable region of 16s rrna of two bacterial species capable of aromatic hydrocarbon metabolism, pseudomonas putida f1 and burkholderia sp. strain js150, and a third, bacillus subtilis atcc 7003, that can function as a secondary degrader. fluorescent in situ hybridization (fish) with species-specific oligonucleotides was used for direct counting of these species throughout a phenol biodegradation experiment in batch culture. traditional di ... | 1998 | 9835594 |
| psychrotolerant bacteria isolated from arctic soil that degrade polychlorinated biphenyls at low temperatures | psychrotolerant polychlorinated biphenyl (pcb)-degrading bacteria were isolated at 7 degreesc from pcb-contaminated arctic soil by using biphenyl as the sole organic carbon source. these isolates were distinguished from each other by differences in substrates that supported growth and substrates that were oxidized. 16s ribosomal dna sequences suggest that these isolates are most closely related to the genus pseudomonas. total removal of aroclor 1242, and rates of removal of selected pcb congener ... | 1998 | 9835569 |
| characteristics of phenanthrene-degrading bacteria isolated from soils contaminated with polycyclic aromatic hydrocarbons. | ten bacterial strains were isolated from seven contaminated soils by enrichment with phenanthrene as the sole carbon source. these isolates and another phenanthrene-degrading strain were examined for various characteristics related to phenanthrene degradation and their ability to metabolize 12 other polycyclic aromatic hydrocarbons (pah), ranging in size from two to five rings, after growth in the presence of phenanthrene. fatty acid methyl ester analysis indicated that at least five genera (agr ... | 1998 | 9830104 |
| biodegradation pathway of 2-chlorodibenzo-p-dioxin and 2-chlorodibenzofuran in the biphenyl-utilising strain jb1. | the biphenyl-utilising burkholderia (previously alcaligenes) strain jb1 is also able to degrade a number of chlorinated dibenzo-p-dioxins and dibenzofurans. in this study, 4-chlorocatechol and a chlorotrihydroxydiphenyl ether were identified as metabolites of 2-chlorodibenzo-p-dioxin. 5-chlorosalicylic acid and a chlorotrihydroxybiphenyl were metabolites of 2-chlorodibenzofuran. these results show that degradation of these compounds follows pathways in which the initial reaction is angular dioxy ... | 1998 | 9828319 |
| [database on nucleotide sequences used as primers of microorganisms]. | the data base (db) "primers of microorganisms" for the accumulation and systematization of information on oligonucleotide sequences, used as primers in polymerase chain reaction, has been created. this db includes data on primers for the laboratory diagnostics of 20 bacterial genera (aerococcus, aeromonas, bartonella, borrelia, burkholderia, chlamydia, clostridium, corynebacterium, escherichia, francisella, helicobacter, legionella, listeria, mycobacterium, mycoplasma, salmonella, shigella, stap ... | 1998 | 9825498 |
| nosocomial burkholderia cepacia infection and colonization associated with intrinsically contaminated mouthwash--arizona, 1998. | during august 1996-june 1998, 74 patients at two hospitals in arizona had cultures positive for burkholderia cepacia. most isolates were from the respiratory tracts of patients in intensive-care units (icus). because of the large number of b. cepacia isolates, personnel at both hospitals requested the arizona department of health services assist in an investigation. this report summarizes the results of the investigation. | 1998 | 9822365 |
| subspecific differentiation of burkholderia cepacia isolates in cystic fibrosis. | thirty clinical isolates of burkholderia cepacia from cystic fibrosis (cf) patients in the uk and denmark were characterised, together with other clinical isolates and laboratory strains of b. cepacia, b. gladioli and b. vietnamiensis. outer-membrane protein (omp) profiles were determined, and the organisms were typed genotypically by pulsed-field gel electrophoresis after dna restriction analyses with xbal and drai. this latter method revealed four clusters among the clinical isolates studied; ... | 1998 | 9822299 |
| monomicrobial nonneutrocytic bacterascites due to burkholderia pickettii. | 1998 | 9820433 | |
| prevalence of human melioidosis on hainan island in china. | eight human cases of melioidosis were diagnosed at hainan people's hospital over a period of one year. four of the cases were of septicemia form, while the rest were of chronic form with abscesses in different organs. all the isolates were intrinsically resistant to cefazolin, cefuroxime, and gentamicin, while being rather sensitive to ampicillin/sulbactam, ticarcillin/clavulanic acid, and imipenem. a serological survey of exotoxin antibodies at different farms showed that xinglong was a farm se ... | 1998 | 9802565 |
| isolation of burkholderia cepacia by enrichment. | burkholderia cepacia is a recognised cause of respiratory failure in patients with cystic fibrosis. the value of routine use of selective enrichment broth to increase the yield of b cepacia from cystic fibrosis sputa was investigated. two hundred sputa from 86 adult and paediatric patients were cultured onto b cepacia selective agar and also in enrichment broth. the enrichment broths were subcultured after incubation onto b cepacia selective agar. fourteen sputa from eight patients yielded b cep ... | 1998 | 9797739 |
| ratios of carbon isotopes in microbial lipids as an indicator of substrate usage. | the occurrence and abundance of microbial fatty acids have been used for the identification of microorganisms in microbial communities. however, these fatty acids can also be used as indicators of substrate usage. for this, a systematic investigation of the discrimination of the stable carbon isotopes by different microorganisms is necessary. we grew 11 strains representing major bacterial and fungal species with four different isotopically defined carbon sources and determined the isotope ratio ... | 1998 | 9797266 |
| epidemiological surveillance of melioidosis in singapore. | during the period 1989 to 1996, a total of 372 cases of melioidosis, with 147 deaths, were reported, giving a mean annual incidence rate of 1.7 per 100,000 population and a case-fatality rate of 39.5%. majority (89%) of the clinical cases were confirmed by culture of burkholderia pseudomallei, while the others were presumptive cases based on a single blood specimen with an indirect haemaglutination (iha) antibody titre of > or = 1:16. the highest incidence rate was reported in those aged 45 year ... | 1998 | 9791650 |
| an ultrastructural study of the phagocytosis of burkholderia pseudomallei. | burkholderia pseudomallei causes melioidosis and is believed to be an intracellular pathogen in human and animal disease. the uptake of b. pseudomallei by mouse peritoneal macrophages and cells in tissue culture was examined by electron microscopy. in all the systems studied b. pseudomallei were phagocytosed and apparently inhibited the normal processes of intracellular killing. destruction of the phagosome membrane occurred and the bacteria escaped into the cytoplasm. | 1998 | 9785484 |
| agricultural and medical microbiology: a time for bridging gaps. | 1998 | 9782485 | |
| burkholderia cepacia. management issues and new insights. | although burkholderia cepacia colonizes a relatively small proportion of individuals with cystic fibrosis (cf), it is associated with significant morbidity and mortality, and has had a profound impact on infection control practices. this article reviews the current understanding of the epidemiology of b. cepacia infection, describes important recent developments in the microbiology and taxonomy of this species, and presents issues that remain obstacles to defining the optimal management of b. ce ... | 1998 | 9759550 |
| evidence for interspecies gene transfer in the evolution of 2,4-dichlorophenoxyacetic acid degraders. | small-subunit ribosomal dna (ssu rdna) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-d)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class proteobacteria. to understand the origin of 2,4-d degradation in this diverse collection, the first gene in the 2,4-d pathway, tfda, was sequenced. the sequences fell into three unique classes found in various members of the beta and ... | 1998 | 9758850 |
| characterization of genes involved in biosynthesis of a novel antibiotic from burkholderia cepacia bc11 and their role in biological control of rhizoctonia solani. | genetic manipulation of fluorescent pseudomonads has provided major insight into their production of antifungal molecules and their role in biological control of plant disease. burkholderia cepacia also produces antifungal activities, but its biological control activity is much less well characterized, in part due to difficulties in applying genetic tools. here we report genetic and biochemical characterization of a soil isolate of b. cepacia relating to its production of an unusual antibiotic t ... | 1998 | 9758823 |
| optimization of differential display of prokaryotic mrna: application to pure culture and soil microcosms. | the differential display (dd) technique, which is widely used almost exclusively for eukaryotic gene discovery, was optimized to detect differential mrna transcription from both pure-culture and soil-derived bacterial rna. a model system which included toluene induction of todc1 in pseudomonas putida f1 was used to optimize the procedure. at 24-h tod induction was determined to be approximately 8 x 10(7) transcripts/microg or 0.08% of the total mrna. the primer concentration, primer length, anne ... | 1998 | 9758787 |
| hla-dr and -dq associations with melioidosis. | melioidosis is an important infectious disease of southeast asia caused by an intracellular bacterium, burkholderia pseudomallei. cellular immunity is postulated to play important roles in immunity to melioidosis that may influence the severity and clinical outcome of the disease. the present study was undertaken to investigate possible associations of melioidosis with hla class ii alleles. hla typing of hla-drb1, -dqa1, and -dqb1 was performed using polymerase chain reaction and sequence-specif ... | 1998 | 9757914 |
| [evaluation of antibacterial activities of various antibiotics against glucose non-fermentative gram-negative rods other than pseudomonas aeruginosa]. | mics of piperacillin, sulbactam/cefoperazone, minocycline (mino), gentamicin, amikacin, flomoxef, ceftazidime, cefozopran, cefsulodin and imipenem were determined, against 189 clinical isolated strains of glucose non-fermentative gram-negative rods (nfgnr; acinetobacter baumannii (44), alcaligenes faecalis (5), alcaligenes xylosoxidans (25), burkholderia cepacia (12), chryseobacterium indologenes (23), chryseobacterium meningosepticum (9), pseudomonas fluorescens (8), pseudomonas putida (12), st ... | 1998 | 9755431 |
| macrophage-lymphocyte interactions mediate anti-burkholderia pseudomallei activity. | the mechanisms involved in the pathogenesis of melioidosis, caused by the intracellular bacterium burkholderia pseudomallei, are unclear. c57bl/6 mice are resistant to infection, while balb/c mice are highly susceptible. previous studies have demonstrated that peritoneal exudate cell preparations enriched for macrophages are capable of effectively eliminating intracellular pathogens. in this study we present evidence showing that interaction of macrophages with lymphocytes is necessary for effic ... | 1998 | 9753000 |
| the diversity of lipases from psychrotrophic strains of pseudomonas: a novel lipase from a highly lipolytic strain of pseudomonas fluorescens. | strains of pseudomonas fluorescens and ps. fragi are the predominant psychrotrophs found in raw milk and may cause spoilage due to the secretion of hydrolytic enzymes such as lipase and protease. the diversity of lipases has been examined in pseudomonas isolates from raw milk which represent different taxonomic groups (phenons). significant diversity was found using both dna hybridization and immunoblotting techniques, which has implications for the development of a diagnostic test. the lipase-e ... | 1998 | 9750283 |
| generation of a reproducible nutrient-depleted biofilm of escherichia coli and burkholderia cepacia. | an in vitro method of growing bacteria as a defined nutrient-depleted biofilm is proposed. the medium was defined nutritionally in terms of the quantitative composition and by the total amount of nutrient required to achieve a defined population size. escherichia coli and burkholderia cepacia were incubated on a filter support placed on a defined volume of solid medium. the change of biomass of the biofilm population was compared with the change in a planktonic culture. the size of the populatio ... | 1998 | 9750276 |
| [analysis of transmission of burkholderia cepacia isolates in an intrahospital by randomly amplified polymorphic dna-pcr method]. | strains of burkholderia cepacia isolated in our hospital from november 1995 to september 1996 were classified with randomly amplified polymorphic dna-pcr (rapd-pcr) and conventional biochemical tests (id test.nf-18, api20ne, and neg combo 4j kit), and intrahospital isolates of b. cepacia were analysed. during the period 28 strains from inpatients and 2 from medical apparatus were isolated. twenty four of 28 (85.7%) were from sputum. in 1996 from january to february, 20 strains were detected from ... | 1998 | 9745217 |
| the o7 antigen of stenotrophomonas maltophilia is a linear d-rhamnan with a trisaccharide repeating unit that is also present in polymers for some pseudomonas and burkholderia species. | the o antigen polymer recovered from the reference strain for stenotrophomonas (xanthomonas or pseudomonas) maltophilia serogroup o7, after mild acid hydrolysis of the lipopolysaccharide, was constructed from d-rhamnose. by means of chemical degradations and nmr studies, the repeating unit of the polymer was shown to be a linear trisaccharide with the structure -->2)-alpha-d-rhap-(1-->3)-alpha-d-rhap-(1-->3)-alpha-d-r hap-(1-->. the same repeating unit is present in the common antigen of pseudom ... | 1998 | 9741084 |
| bacterial pathogens (non-mycobacterium) from sputum culture and antimicrobial susceptibility. | sputum culture of patients at siriraj hospital, bangkok was 49.84% positive for bacterial pathogens in 1994 and 40.95% in 1995. the average incidence of gram-negative rods was 3.11 fold more than the combination of gram-positive cocci and gram-negative cocci. the most common gram-negative rod was pseudomonas aeruginosa, followed by either klebsiella pneumoniae or acinetobacter anitratus depending on year. the most common coccus was staphylococcus aureus. from both years, the number of haemophilu ... | 1998 | 9740278 |
| total nutrient admixtures appear safer than lipid emulsion alone as regards microbial contamination: growth properties of microbial pathogens at room temperature. | the extraordinary growth properties of most microorganisms in 10% and 20% lipid emulsions has led to the centers for disease control and prevention recommendation that if lipids are given through an i.v. line, the administration set should be replaced every 24 hours rather than the usual 72-hour interval used for crystalloid solutions, including those used for conventional total parenteral nutrition. for nearly 15 years, parenteral alimentation has been given as a total nutrient admixture (tna), ... | 1998 | 9739032 |
| in-vitro investigation of the antibacterial activity of agents which may be used for the oral treatment of lung infections in cf patients. | the effect of triple therapy with ciprofloxacin, trimethoprim and either sulphadiazine or sulphamethoxazole on the mics and development of resistance of three strains of pseudomonas aeruginosa, two strains of staphylococcus aureus and one of burkholderia cepacia was compared with that of single or dual therapy with these agents using an agar dilution method. ciprofloxacin mics were 0.2-0.8 mg/l for the p. aeruginosa and s. aureus strains. for trimethoprim the mic ranges were 64-128 and 0.25-1 mg ... | 1998 | 9738834 |
| increasing resistance of planktonic and biofilm cultures of burkholderia cepacia to ciprofloxacin and ceftazidime during exponential growth. | the change in resistance of burkholderia cepacia to ceftazidime and to ciprofloxacin during the exponential phase and up to the onset of stationary phase was assessed along the growth curve in batch culture. b. cepacia was grown in planktonic culture and in a biofilm on a membrane support. resistance increased progressively during the exponential phase, being increased by ten-fold about every four generations. bacteria grown in a biofilm were about 15 times more resistant than equivalent plankto ... | 1998 | 9738832 |
| outbreak of pseudomonas fluorescens bacteremia among oncology patients. | from 7 to 24 march 1997, four patients developed pseudomonas fluorescens bacteremia at the hospital; one on the oncology ward and the other three in the chemotherapy room. these patients all had underlying malignancies and had the port-a-cath (smiths industries medical systems, deltec, inc., st. paul, minn.) implants. three patients had primary bacteremia, and one had port-a-cath-related infection. none of these patients had received a blood transfusion before the episodes of bacteremia. all pat ... | 1998 | 9738043 |
| activities of ll-37, a cathelin-associated antimicrobial peptide of human neutrophils. | human neutrophils contain two structurally distinct types of antimicrobial peptides, beta-sheet defensins (hnp-1 to hnp-4) and the alpha-helical peptide ll-37. we used radial diffusion assays and an improved national committee for clinical laboratory standards-type broth microdilution assay to compare the antimicrobial properties of ll-37, hnp-1, and protegrin (pg-1). although generally less potent than pg-1, ll-37 showed considerable activity (mic, <10 microgram/ml) against pseudomonas aerugino ... | 1998 | 9736536 |
| channel specificity and secondary structure of the glucose-inducible porins of pseudomonas spp. | the oprb porin-mediated glucose transport system was investigated in pseudomonas chlororaphis, burkholderia cepacia, and pseudomonas fluorescens. kinetic studies of [u-14c]glucose uptake revealed an inducible system of low km values (0.3-5 microm) and high specificity for glucose. oprb homologs were purified and reconstituted into proteoliposomes. the porin function and channel preference for glucose were demonstrated by liposome swelling assays. examination of the periplasmic glucose-binding pr ... | 1998 | 9733092 |
| burkholderia graminis sp. nov., a rhizospheric burkholderia species, and reassessment of [pseudomonas] phenazinium, [pseudomonas] pyrrocinia and [pseudomonas] glathei as burkholderia. | in a survey of soil and wheat or maize rhizoplane bacteria isolated using a medium containing azelaic acid and tryptamine as sole carbon and nitrogen sources, respectively, a large proportion of burkholderia-like bacteria were found. among them, a homogeneous group of strains was identifiable based on phenotypic properties, fatty acid composition, dna-dna hybridizations and 16s rdna sequences. according to molecular data, this group belongs to the genus burkholderia but its weak similarity to pr ... | 1998 | 9731297 |
| change in bacterial community during biodegradation of aniline. | the response of river water microbial communities to chemical compounds was monitored under laboratory conditions using aniline as a model. bacteria were collected from unpolluted and polluted sites. bacterial abundance (plate and total direct counting) and its relation to aniline biodegradation was examined. colony hybridization with 16s rrna oligonucleotide probes was used to study the changes in microbial community structure during biodegradation of aniline. the changes in bacterial abundance ... | 1998 | 9729107 |
| killing of pseudomonas pseudomallei by polymorphonuclear leukocytes and peritoneal macrophages from chicken, sheep, swine and rabbits. | differences in the kinetics of pseudomonas pseudomallei killing by peritoneal macrophages (pm) and polymorphonuclear leucocytes (pmnl) from chickens, sheep, swine and rabbits were found. p. pseudomallei was rapidly killed by porcine pm and pmnl. however the bacterial killing by ovine and lapine pm and pmnl proceeded at a slower rate. in contrast, chicken pm and pmnl ingested and killed the lowest number of p. pseudomallei bacteria. the differences in the bactericidal activity of pm and pmnl from ... | 1998 | 9728410 |
| pseudomonas aeruginosa and burkholderia cepacia infection in cystic fibrosis patients treated in toronto and copenhagen. | differences in the course of pulmonary disease in cystic fibrosis (cf) may be altered by different treatment strategies in different cf centers. the copenhagen clinic uses scheduled, regular and very aggressive treatment of lung infection. the toronto clinic treats pulmonary infection with oral, inhaled, or intravenous antibiotics, and has emphasized aggressive nutritional therapy. this study compared the clinical status of cf patients treated in the two centers (toronto, canada, n=302, and cope ... | 1998 | 9727758 |
| significance of airway colonization by burkholderia gladioli in lung transplant candidates. | 1998 | 9726771 | |
| purification and preliminary characterization of a serine hydrolase involved in the microbial degradation of polychlorinated biphenyls. | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (6-phenyl-hoda) hydrolase (bphd), an enzyme of the biphenyl biodegradation pathway encoded by the bphd gene of burkholderia cepacia lb400, was hyperexpressed and purified to apparent homogeneity. sds-polyacrylamide gel electrophoresis confirmed that bphd has a subunit molecular mass of 32 kda, while gel filtration demonstrated that it is a homotetramer of molecular weight 122,000. the enzyme hydrolyzed 6-phenyl-hoda with a kcat of 5.0 (+/- 0.07) s-1 and ... | 1998 | 9722515 |
| pyrrolnitrin from burkholderia cepacia: antibiotic activity against fungi and novel activities against streptomycetes. | a bacterial strain identified as burkholderia cepacia nb-1 was isolated from water ponds in the botanical garden in tübingen, germany, and was found to produce a broad spectrum phenylpyrrole antimicrobial substance active against filamentous fungi, yeasts and gram-positive bacteria. in batch culture containing glycerol and l-glutamic acid, the isolate nb-1 produced the antibiotic optimally late in the growth phase and accumulated a main portion in their cells. isolation and purification of the a ... | 1998 | 9721657 |
| novel pathway for conversion of chlorohydroxyquinol to maleylacetate in burkholderia cepacia ac1100. | burkholderia cepacia ac1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) via formation of 5-chlorohydroxyquinol (5-chq), hydroxyquinol (hq), maleylacetate, and beta-oxoadipate. the step(s) leading to the dechlorination of 5-chq to hq has remained unidentified. we demonstrate that a dechlorinating enzyme, tftg, catalyzes the conversion of 5-chq to hydroxybenzoquinone, which is then reduced to hq by a hydroxybenzoquinone reductase (hbq reductase). hq is subsequently converted to maleyla ... | 1998 | 9721310 |
| rrna-targeted fluorescent in situ hybridization analysis of bacterial community structure in river water. | an improved in situ hybridization technique, hnpp-fish, using 2-hydroxy-3-naphthoic acid 2'-phenylanilide phosphate (hnpp) and fast red tr was applied to analyse the community structure of planktonic bacteria in river water. oligonucleotide probes specific for the domain bacteria (eub338) and five bacterial groups [flavobacterium-cytophaga; burkholderia-pseudomonas (rrna iii)-authentic alcaligenes; vibrio-aeromonas; pseudomonas (rrna i): the genus acinetobacter] were used to investigate the bact ... | 1998 | 9720029 |
| microbial communities of printing paper machines. | the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ... | 1998 | 9717292 |
| clinical problems posed by multiresistant nonfermenting gram-negative pathogens. | in this review i will briefly survey the role of pseudomonas aeruginosa, acinetobacter baumanii, stenotrophomonas maltophilia, and burkholderia cepacia as opportunistic pathogens. a common feature of these organisms is intrinsic resistance to multiple antibiotics. all of these organisms can be recovered from the environment, commonly cause device-related infections, are often resistant to disinfectants, and have the potential to spread from patient to patient via fomites or the hands of medical ... | 1998 | 9710680 |
| resistance mechanisms in pseudomonas aeruginosa and other nonfermentative gram-negative bacteria. | nonfermentative gram-negative bacilli are still a major concern in compromised individuals. by far the most important of these organisms is pseudomonas aeruginosa, although acinetobacter baumannii (previously acinetobacter calcoaceticus), stenotrophomonas maltophilia (previously pseudomonas and xanthomonas maltophilia), and burkholderia cepacia (previously pseudomonas cepacia) are also of substantative concern because of their similar high intrinsic resistances to antibiotics. the basis for the ... | 1998 | 9710677 |
| cross infection between cystic fibrosis patients colonised with burkholderia cepacia. | whilst patient to patient spread of the respiratory pathogen burkholderia cepacia is well recognised between patients with cystic fibrosis, prompting a strict segregation policy, cross colonisation between cystic fibrosis patients already infected with b cepacia has not been described and surveys show a very low incidence of patients with more than one strain. five adult cystic fibrosis patients with b cepacia are presented who became cross colonised with a second b cepacia (uk epidemic) strain, ... | 1998 | 9708241 |
| chronic burkholderia cepacia bronchiectasis in a non-cystic fibrosis individual. | infection with burkholderia cepacia due to social contact is well described in patients with cystic fibrosis. however, social transmission to non-cystic fibrosis individuals or chronic colonisation in non-cystic fibrosis individuals has not been described. a report of b cepacia bronchiectasis is presented where a previously healthy mother of two cystic fibrosis children colonised with b cepacia became infected by the same epidemic strain. the implications of this for parents, siblings, and partn ... | 1998 | 9708240 |
| burkholderia cepacia: another twist and a further threat. | 1998 | 9708220 | |
| discrimination of burkholderia gladioli from other burkholderia species detectable in cystic fibrosis patients by pcr. | a procedure for molecular identification of burkholderia gladioli is described. specific 16s and 23s rrna gene signature sequences were defined as primers for pcr. the method allows rapid and specific discrimination of b. gladioli from related species (b. cepacia, b. multivorans, b. vietnamiensis, b. mallei, b. pseudomallei, ralstonia pickettii, and r. eutropha) and should contribute to the clarification of its role as a human pathogen, e.g., in cystic fibrosis. | 1998 | 9705429 |
| molecular procedure for rapid detection of burkholderia mallei and burkholderia pseudomallei. | a pcr procedure for the discrimination of burkholderia mallei and burkholderia pseudomallei was developed. it is based on the nucleotide difference t 2143 c (t versus c at position 2143) between b. mallei and b. pseudomallei detected in the 23s rdna sequences. in comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel. | 1998 | 9705426 |
| stability of strain genotypes of burkholderia pseudomallei from patients with single and recurrent episodes of melioidosis. | the constancy of strain genotypes of multiple isolates of burkholderia pseudomallei from 13 patients with melioidosis was examined by bamhi ribotyping and pulsed-field gel electrophoresis (pfge) of xbai digests of dna. seven of 8 patients with single episodes of melioidosis each yielded genetically identical isolates and only one of five patients with recurrent episodes was infected with a new strain clearly distinct from the original primary strain. variation was observed in pfge patterns of pr ... | 1998 | 9705184 |
| aetiology and outcome of severe community-acquired pneumonia in singapore. | the aim of this study was to determine the aetiology and outcome of severe community-acquired pneumonia, and to assess whether the existing guidelines for initial antimicrobial therapy are being applied. the records of 57 consecutive nonimmunocompromised patients admitted to the medical intensive care unit (icu) between january 1989 and may 1993 with this diagnosis were reviewed. the microbiological data, chest radiographic changes and outcome were analysed. nine (16%) of the 57 patients had pul ... | 1998 | 9701424 |
| activity of cefepime compared with other antibiotics against gram-positive bacteria and cefuroxime-resistant gram-negative bacteria. | cefepime is a new, parenteral, fourth-generation antibiotic that is stable in the presence of bush group 1 beta-lactamases. in vitro activity of cefepime, cefuroxime, ceftazidime, ciprofloxacin and imipenem against gram-positive cocci and cefuroxime-resistant gram-negative bacilli was studied. | 1998 | 9699393 |
| phenotypic variation of lipid composition in burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid. | burkholderia cepacia produces an unusual range of polar lipids, which includes two forms each of phosphatidylethanolamine (pe) and ornithine amide lipid (ol), differing in the presence or absence of 2-hydroxy fatty acids. by using chemostat cultures in chemically defined media, variations in the lipid content and the proportions of individual lipids have been studied as a function of (a) growth temperature, (b) growth rate and (c) growth-limiting nutrient (carbon, magnesium, phosphorus or oxygen ... | 1998 | 9695908 |
| detection of a new enzyme for stereoselective hydrolysis of linalyl acetate using simple plate assays for the characterization of cloned esterases from burkholderia gladioli. | plate assays were developed for the identification of specific hydrolytic activities of esterases from burkholderia gladioli, cloned and expressed in escherichia coli. clones showing different substrate specificities were identified by fluorescence or azo-dye formation caused by the released alcohol moiety of the hydrolyzed substrates, or by colour change of ph indicators mediated by decreased ph. the use of 1-hydroxypyrene-3,6,8-trisulfonic acid (hpts-) esters and linalyl acetate for these assa ... | 1998 | 9684341 |
| chemo-enzymatic synthesis of a new type of enantiomerically pure carbocyclic nucleoside analogues with strong inhibitory effects on terminal deoxynucleotidyl transferase. | the synthesis of enantiomerically pure carbocyclic adenosine derivatives which have been prepared based on the kinetic resolution of a trans-2-(hydroxymethyl)cyclopentanol derivative is described. their corresponding triphosphates were evaluated as inhibitors of dna polymerase beta, terminal deoxynucleotidyl transferase (tdt), telomerase, escherichia coli dna polymerase i and reverse transcriptase of human immunodeficiency virus. surprisingly, the triphosphate of (1s,2r)-1-(6-aminopurin-9-yl)-2- ... | 1998 | 9681136 |
| distribution of mevalonate and glyceraldehyde 3-phosphate/pyruvate routes for isoprenoid biosynthesis in some gram-negative bacteria and mycobacteria. | labeling experiments using [1-13c]acetate or [1-13c]glucose were performed with opportunistic pathogenic bacteria, with innocuous bacteria related to pathogenic species or with phytopathogenic species. the labeling pattern was determined in the isoprenic moiety of ubiquinone or menaquinone derivatives. these experiments showed that acinetobacter, citrobacter, erwinia, pseudomonas, burkholderia, ralstonia and mycobacterium synthesize their isoprenoids via the mevalonate-independent glyceraldehyde ... | 1998 | 9675863 |
| microbiology of sputum from patients at cystic fibrosis centers in the united states. | during a phase iii national collaborative study of aerosolized tobramycin (1 july 1995 through 30 september 1996), the microbiology of specimens from 595 patients at 69 cystic fibrosis (cf) centers was examined. samples from three screening visits were processed in a single laboratory by means of standardized techniques for identification and susceptibility testing. from 1,753 pretreatment specimens, 5,128 pathogens were isolated (average, 2.9/specimen). of the 3,936 pseudomonas aeruginosa isola ... | 1998 | 9675470 |
| structural determination of the o-deacetylated o-chain of lipopolysaccharide from burkholderia (pseudomonas) cepacia strain pvfi-5a. | on the basis of chemical degradation methods and one-and two-dimensional 1h and 13c nmr experiments the novel following structure was established for the o-deacetylated repeating unit of the o-chain of the main burkholderia (pseudomonas) cepacia (strain pvfi-5a) lipopolysaccharide: -->4)-beta-d-galpnac-(1-->3)-alpha-d-galp-(1-->6)-alpha-d-glcpnac-(1-->. | 1998 | 9675371 |