Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| outbreak of carbapenem-resistant klebsiella pneumoniae producing kpc-3 in a tertiary medical centre in israel. | this report describes an outbreak of carbapenem-resistant kpc-3-producing klebsiella pneumoniae outside the usa. ninety patients from different departments of a tertiary medical centre were diagnosed with carbapenem-resistant, extended-spectrum beta-lactamase (esbl)-negative klebsiella pneumoniae infection by standard methods over a 10-month period in 2006. fifteen randomly selected outbreak isolates were subjected to randomly amplified polymorphic dna (rapd) polymerase chain reaction (pcr) as w ... | 2007 | 17931835 |
| evaluation of antimicrobial susceptibility for beta-lactams using the etest method against clinical isolates from 100 medical centers in japan (2006). | this antimicrobial resistance surveillance study was performed in 100 medical centers. susceptibility testing (etest; ab biodisk, solna, sweden) of 9152 strains including escherichia coli (991 strains), klebsiella spp. (1000 strains), enterobacter spp. (971 strains), citrobacter spp. (803 strains), indole-positive proteae spp. (834 strains), serratia spp. (902 strains), acinetobacter spp. (874 strains), pseudomonas aeruginosa (992 strains), oxacillin-susceptible staphylococcus aureus (984 strain ... | 2008 | 17931820 |
| molecular epidemiology of two klebsiella pneumoniae mastitis outbreaks on a dairy farm in new york state. | klebsiella spp. have become an important cause of clinical mastitis in dairy cows in new york state. we describe the occurrence of two klebsiella mastitis outbreaks on a single dairy farm. klebsiella isolates from milk, feces, and environmental sources were compared using random amplified polymorphic dna (rapd)-pcr typing. the first mastitis outbreak was caused by a single strain of klebsiella pneumoniae, rapd type a, which was detected in milk from eight cows. rapd type a was also isolated from ... | 2007 | 17928424 |
| prevalence of nosocomial infection in thailand 2006. | to study the prevalence of nosocomial infection (ni). | 2007 | 17926980 |
| outcome of escherichia coli and/or klebsiella bloodstream infection in children with central venous catheters. | we conducted a retrospective cohort study of children with catheter-associated bloodstream infections (bsis) due to escherichia coli and/or klebsiella. risk factors for poor outcome (ie, death or recurrence of infection) were receipt of mechanical ventilation (adjusted odds ratio [aor], 4.6 [95% confidence interval {ci}, 1.39-16.30]) and receipt of total parenteral nutrition (aor, 3.5 [95% ci, 1.1-10.8]). a significant proportion of children with catheter-associated bsi were treated successfully ... | 2007 | 17926284 |
| increasing prevalence of gastrointestinal colonization with ceftazidime-resistant gram-negative bacteria among intensive care unit patients. | the occurrence of nosocomial infections due to third-generation cephalosporin-resistant gram-negative bacteria is increasing. gastrointestinal colonization is an important reservoir for antibiotic-resistant bacteria, and it often precedes clinical infection. | 2007 | 17926274 |
| bacterial contaminations of informally marketed raw milk in ghana. | summary | 2007 | 17925843 |
| [association of tracheobronchopathia osteochondroplastica and ozène]. | we report a case of tracheobronchopathia osteochondroplastica associated with ozena (atrophic rhinitis). | 2007 | 17925671 |
| synthesis, antibacterial activity, and quantitative structure-activity relationships of new (z)-2-(nitroimidazolylmethylene)-3(2h)-benzofuranone derivatives. | a new series of (z)-2-(1-methyl-5-nitroimidazole-2-ylmethylene)-3(2h)-benzofuranones (11a-p) and (z)-2-(1-methyl-4-nitroimidazole-5-ylmethylene)-3(2h)-benzofuranones (12a-m) were synthesized and assayed for their antibacterial activity against gram-positive and gram-negative bacteria. most of the 5-nitroimidazole analogues (11a-p) showed a remarkable inhibition of a wide spectrum of gram-positive bacteria (staphylococcus aureus, streptococcus epidermidis, mrsa, and bacillus subtilis) and gram-ne ... | 2007 | 17919903 |
| complex clonal and plasmid epidemiology in the first outbreak of enterobacteriaceae infection involving vim-1 metallo-beta-lactamase in spain: toward endemicity? | we report the emergence and spread of metallo-beta-lactamases (mbls) among enterobacterial isolates at ramón y cajal university hospital (madrid, spain). | 2007 | 17918078 |
| salmonella-a new threat to neonates. | hospital admitted 582 neonates with suspected septicaemia were studied in microbiology department of dhaka medical college, dhaka, bangladesh. samples being taken from neonatal unit of dhaka medical college hospital and a neonatal private hospital in one year (january to december 2004). blood culture was done by lytic-centrifugation method. the isolated organisms were identified using standard laboratory procedures. among 582 sick neonates 59(10.14%) were culture positive and the predominant org ... | 2007 | 17917624 |
| [pyogenic liver abscess complicated by gastric fistula and bacterial meningitis]. | a 77-year-old woman was admitted suffering from fever and headache. on laboratory examination, bacterial meningitis and sepsis due to klebsiella pneumoniae were diagnosed. in addition, a hepatic cystic lesion measuring 13 cm in diameter in the left lobe was indicated on diagnostic imaging. after treatment with antibiotics, her signs of infection improved and the hepatic lesion decreased in size. after discharge, however, the cystic liver mass increased and a gastric fistula developed. hepatic an ... | 2007 | 17917400 |
| gene cloning and characterization of kdea, a multidrug efflux pump from klebsiella pneumoniae. | we cloned a gene related to multidrug resistance from a drug-resistant klebsiella pneumoniae strain mgh78578. we designated the gene kdea, which encodes a protein possessing 12 hydrophobic regions. the deduced amino acid sequence of kdea is similar to that of mdfa, a well-characterized multidrug efflux pump from escherichia coli. introduction of the kdea gene into cells of the drug-hypersusceptible e. coli strain kam32 resulted in elevated minimum inhibitory concentrations of chloramphenicol, no ... | 2007 | 17917272 |
| do anterior cruciate ligament allograft culture results correlate with clinical infections? | in 1998, four cases of contaminated allografts for anterior cruciate ligament (acl) reconstruction resulted in clostridium infection, and a patient with clostridium infection from a femoral condylar allograft died. it was subsequently published that implanting surgeons should culture acl allografts so that action could be taken should highly pathogenic bacteria be encountered. the purpose of this study is to test the hypothesis that acl allograft cultures correlate with clinical infections. | 2007 | 17916476 |
| molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases. | adenosylcobalamin-dependent diol and glycerol dehydratases are isofunctional enzymes and undergo mechanism-based inactivation by a physiological substrate glycerol during catalysis. inactivated holoenzymes are reactivated by their own reactivating factors that mediate the atp-dependent exchange of an enzyme-bound, damaged cofactor for free adenosylcobalamin through intermediary formation of apoenzyme. the reactivation takes place in two steps: (a) adp-dependent cobalamin release and (b) atp-depe ... | 2007 | 17916188 |
| production of 1,3-propanediol from glycerol by recombinant e. coli using incompatible plasmids system. | 1,3-propanediol (1,3-pd) has numerous applications in polymers, cosmetics, foods, lubricants, and medicines as a bifunctional organic compound. the genes for the production of 1,3-pd in klebsiella pneumoniae, dhab, which encodes glycerol dehydratase, and dhat, which encodes 1,3-pd oxidoreductase, and gdrab, which encodes glycerol dehydratase reactivating factor, are naturally under the control of different promoters and are transcribed in different directions. these genes were coexpressed in e. ... | 2007 | 17914171 |
| evaluation of the chromogenic cica-beta-test for detecting extended-spectrum, ampc and metallo-beta-lactamases. | extended-spectrum, metallo- and ampc beta-lactamases usually are sought subsequently to susceptibility testing, meaning that producers are not identified until 72 h after a clinical specimen is taken. chromogenic tests might usefully shorten this delay, and we investigated the cica-beta-test for this purpose. | 2007 | 17913719 |
| prevalence of clinical isolates of escherichia coli and klebsiella spp. producing multiple extended-spectrum beta-lactamases. | eleven thousand two hundred seventy-two escherichia coli, 1109 klebsiella pneumoniae, 1124 salmonella enterica, and 602 klebsiella oxytoca unrelated clinical isolates were obtained between 2001 and 2004 in a university hospital in salamanca, spain. one hundred thirteen e. coli (1%), 32 k. pneumoniae (2.9%), 4 k. oxytoca (0.66%), and 5 s. enterica (0.44%) isolates produced extended-spectrum beta-lactamases (esbls). we obtained 42.2% of the esbl-producing isolates from outpatients and 57.8% from i ... | 2007 | 17913435 |
| medicinal smoke reduces airborne bacteria. | this study represents a comprehensive analysis and scientific validation of our ancient knowledge about the effect of ethnopharmacological aspects of natural products' smoke for therapy and health care on airborne bacterial composition and dynamics, using the biolog microplate panels and microlog database. we have observed that 1h treatment of medicinal smoke emanated by burning wood and a mixture of odoriferous and medicinal herbs (havan sámagri=material used in oblation to fire all over india) ... | 2007 | 17913417 |
| antimicrobial activity of some salvia species essential oils from iran. | the aerial parts of salvia multicaulis, s. sclarea and s. verticillata were collected at full flowering stage. the essential oils were isolated by hydrodistillation and analyzed by combination of capillary gc and gc-ms. the in vitro antimicrobial activity of the essential oils were studied against eight gram-positive and gram-negative bacteria (bacillus subtilis, bacillus pumulis, enterococcus faecalis, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, pseudomonas aeruginosa a ... | 2007 | 17913065 |
| b27 disease is a new autoimmune disease that affects millions of people. | "b27 disease" is a new autoimmune disease that afflicts millions of people throughout the world. "b27 disease" occurs in individuals who have ankylosing spondylitis (as) or preankylosing spondylitis and/or uveitis and are also positive for hla-b27. molecular mimicry between the bowel microbe klebsiella and the hla-b27 molecule, as well as the spinal collagens types i, iii, and iv, indicates a pathological mechanism involving autoimmunity. antibodies to klebsiella microbes have been reported in a ... | 2007 | 17911426 |
| bacteriologic comparison of tonsil core in recurrent tonsillitis and tonsillar hypertrophy. | although many bacteriology studies on tonsillar diseases have been completed, all have been confined to children and were characterized by a paucity of cases. the purpose of this study was to analyze the underlying bacterial pathogens in tonsillar disease. | 2007 | 17909446 |
| abscess-a rare fourth ventricular mass. | midline posterior fossa mass lesions in young adults are usually medulloblastomas, pilocytic astrocytomas, haemangioblastomas or ependymomas. we present clinical, imaging and intraoperative findings of an abscess occurring within the fourth ventricle which was associated with right chronic suppurative otitis media and right transverse sinus thrombosis. the possible pathogenesis is discussed. | 2007 | 17906968 |
| pattern of pathogens in ear discharge of hiv-infected children in nnewi, southeast nigeria. | this is a descriptive, prospective, hospital-based study designed to determine the prevalent organisms in ear infections and their antimicrobial susceptibility patterns in hiv-infected children compared to age-and sex-matched hiv sero-negative patients. | 2007 | 17902505 |
| one year trends in the gram-negative bacterial antibiotic susceptibility patterns in a medical intensive care unit in south india. | to describe the changes in antibiotic susceptibility patterns of common intensive care unit pathogens with time from the medical intensive care unit of a tertiary care hospital. | 2007 | 17901640 |
| outlined psoas muscle shadow. | 2007 | 17901289 | |
| dry stress and survival time of enterobacter sakazakii and other enterobacteriaceae in dehydrated powdered infant formula. | powdered infant formula is not a sterile product, and opportunistic pathogens could multiply in the reconstituted product, resulting in neonatal infections. in this study, the generation of sublethally injured enterobacteriaceae during desiccation and their persistence in dehydrated powdered infant formula was assessed during a 2.5-year period. the study included 27 strains of enterobacter sakazakii, enterobacter cloacae, salmonella enteritidis, citrobacter koseri, citrobacter freundii, escheric ... | 2007 | 17900090 |
| acute bacterial meningitis in nepal. | this study was conducted at tribhuvan university teaching hospital, a tertiary care hospital in nepal from january 2001 to march 2002. the prevalence of bacterial meningitis, its causative organisms and their antibiotic sensitivity patterns were studied in cerebrospinal fluid of 448 suspected cases. the prevalence of acute bacterial meningitis was 8.3%. there was no significant association (p > 0.05) of the disease with gender (x2 = 0.0234) and among different age groups (x2 = 6.07875) studied. ... | 2007 | 17899958 |
| the use of ultrasonic imaging to evaluate the effect of protozoan grazing and movement on the topography of bacterial biofilms. | this study evaluated the effect of protozoan movement and grazing on the topography of a dual-bacterial biofilm using both conventional light microscopy and a new ultrasonic technique. | 2007 | 17897377 |
| endophytic colonization of typha australis by a plant growth-promoting bacterium klebsiella oxytoca strain gr-3. | to isolate and characterize endophytic diazotrophic bacteria from a semi-aquatic grass (typha australis) which grows luxuriantly with no addition of any nitrogen source. | 2007 | 17897235 |
| antimicrobial activity and the major components of the essential oil of plectranthus cylindraceus. | the traditional uses of the aerial parts of fresh plectranthus cylindraceus by the dhofaris in oman were investigated on the basis of antimicrobial properties and composition of its herb oil. | 2007 | 17897226 |
| new ethacridine derivatives as the potential antifungal and antibacterial preparations. | until the 20th century fungal infections were rather easy cured, and the need of new antifungal drugs was low. however, low choice of antifungal preparations, their toxicity, limited spectrum of action, and ability to produce resistant strains show the need of new effective medicines for systemic fungal diseases in nowadays. our goal of research was to synthesize new antimicrobial compounds containing three or more pharmacophores in one molecule. the initial 5-substituted-2-methylmercaptothiazol ... | 2007 | 17895642 |
| vaccination of metastatic colorectal cancer patients with matured dendritic cells loaded with multiple major histocompatibility complex class i peptides. | developing a process to generate dendritic cells (dcs) applicable for multicenter trials would facilitate cancer vaccine development. moreover, targeting multiple antigens with such a vaccine strategy could enhance the efficacy of such a treatment approach. we performed a phase 1/2 clinical trial administering a dc-based vaccine targeting multiple tumor-associated antigens to patients with advanced colorectal cancer (crc). a qualified manufacturing process was used to generate dc from blood mono ... | 2007 | 17893568 |
| synthesis of the 'primer-adaptor' trisaccharide moiety of escherichia coli o8, o9, and o9a lipopolysaccharide. | described is the synthesis of the trisaccharide alpha-d-manp-(1-->3)-alpha-d-manp-(1-->3)-beta-d-glcpnaco(ch2)8n3, the glycan portion of which corresponds to the 'adaptor-primer' moiety linking the o-chain and core oligosaccharide in the lipopolysaccharide of several escherichia coli and klebsiella pneumoniae serotypes. this report represents the first synthesis of this trisaccharide motif, and in the route involved, a key step is a [2+1] coupling of a protected manp-(1-->3)-alpha-d-manp glycosy ... | 2007 | 17892864 |
| an interlocked dimer of the protelomerase telk distorts dna structure for the formation of hairpin telomeres. | the termini of linear chromosomes are protected by specialized dna structures known as telomeres that also facilitate the complete replication of dna ends. the simplest type of telomere is a covalently closed dna hairpin structure found in linear chromosomes of prokaryotes and viruses. bidirectional replication of a chromosome with hairpin telomeres produces a catenated circular dimer that is subsequently resolved into unit-length chromosomes by a dedicated dna cleavage-rejoining enzyme known as ... | 2007 | 17889664 |
| dark h2 fermentation from sucrose and xylose using h2-producing indigenous bacteria: feasibility and kinetic studies. | cellulosic materials are the major components in biomass feedstock used for bioenergy production. hydrolytic products of cellulosic substances consist primarily of hexose (e.g., glucose) and pentose (e.g., xylose). in this study, the efficiency of fermentative conversion of sucrose (representing hexose) and xylose into h2 was examined with seven h2-producing pure strains isolated from a high-rate h2-producing system in our recent work. the isolates were identified as clostridium butyricum (strai ... | 2008 | 17889245 |
| epidemiology and outcomes of multiple antibiotic-resistant bacterial infection in renal transplantation. | mutiresistant bacterial infections are an emerging problem in the nosocomial setting. our objectives were to describe the incidence, outcome, and risk factors for acquisition of multiresistant bacteria among renal transplant recipients. | 2007 | 17889144 |
| antibiotic resistance surveillance over a 4-year period (2000-2003) in turkey: results of the mystic program. | the meropenem yearly susceptibility test information collection (mystic) program is a global study that provides antimicrobial susceptibility data in centers prescribing meropenem. the activity of meropenem and 7 broad-spectrum antimicrobials have been examined against 5208 bacterial isolates from 9 turkish centers between 2000 and 2003. cumulative susceptibility rates against all species of enterobacteriaceae combined were ranked as follows: meropenem (99.3%), imipenem (97.6%), cefepime (80.0%) ... | 2007 | 17888609 |
| a 7-year national survey on bacterial resistance in bronchoalveolar lavage from patients hospitalized in argentina. | the purpose of this study is to undertake a nationwide survey on bacterial resistance in bronchoalveolar lavage (bal) from patients hospitalized in argentina. a 2-month point prevalence study was conducted twice yearly (april-may and october-november) from 1997 to 2003 by 36 argentinean centers. antimicrobial susceptibility data of the potential pathogens recovered from the bal (samples containing <1% of squamous epithelial cells and bacterial counts >or=10(4) cfu/ml) of inpatients (i.e., >or=48 ... | 2008 | 17888608 |
| test and teach. number 39. diagnosis: rhinoscleroma. | 2007 | 17886103 | |
| risk factors of carbapenem-resistant klebsiella pneumoniae infections: a matched case control study. | carbapenems are frequently used to treat infections due to extended-spectrum beta-lactamase-producing klebsiella pneumoniae. thus, the emergence of infections due to carbapenem-resistant k. pneumoniae (crkp) is a major public health concern. | 2007 | 17884829 |
| risk factors for extended-spectrum beta-lactamase-producing serratia marcescens and klebsiella pneumoniae acquisition in a neonatal intensive care unit. | we investigated the molecular epidemiology of gentamicin-resistant, extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae and serratia marcescens, and risk factors associated with their acquisition in a neonatal intensive care unit (nicu) of a university hospital in italy. during the study period (april-november 2004), s. marcescens was responsible for six infections and 31 colonisations, while k. pneumoniae was responsible for six infections and 103 colonisations. concurrent i ... | 2007 | 17884248 |
| polymeric drug based on sulfanilamide: synthesis, antimicrobial and drug releasing studies. | n-((4-amino sulfonyl)phenyl)acrylamide (apa) was synthesized using sulfanilamide and acryloyl chloride in the presence of triethyl amine at 0-5 degrees c. homo- and co-polymerization of 2-hydroxyethyl acrylate (hea) and acrylic acid (aa) were done by adopting a solution polymerization technique using methyl ethyl ketone (mek) as a solvent and benzoyl peroxide (bpo) as a free radical initiator at 70 +/- 1 degrees c. all the monomers and polymers were characterized by ir and nmr techniques. these ... | 2007 | 17883891 |
| an outbreak of extended spectrum beta-lactamase producing klebsiella pneumoniae in a neonatal intensive care unit. | klebsiella pneumoniae has long been a prominent cause of nosocomial infections and outbreaks have been observed in the intensive care units and in high risk groups. we present here a brief report on an outbreak of klebsiella pneumoniae which occurred in a neonatal intensive care unit in our teaching hospital. as neonates are at highest risk for acquisition of klebsiella pneumoniae producing extended spectrum beta-lactamase, infection control policies and procedures should be strictly followed to ... | 2007 | 17883180 |
| characterization of ctx-m-22 and tem-141 encoded by a single plasmid from a clinical isolate of enterobacter cloacae in china. | we analyzed the resistance to expanded-spectrum cephalosporins of an enterobacter cloacae clinical isolate, ec002, by transconjugation, isoelectric-focusing analysis, and cloning experiments. it produced two beta-lactamases with isoelectric point values of 5.4 and 8.7, corresponding to tem-141, a novel variant of tem-1, and ctx-m-22, encoded by a transferable plasmid. | 2007 | 17881870 |
| evaluation of bacterial inactivation in prestorage pooled, leukoreduced, whole blood-derived platelet concentrates suspended in plasma prepared with photochemical treatment. | photochemical treatment (pct) with amotosalen and ultraviolet light was developed to inactivate pathogens in platelet (plt) components suspended in 35 percent plasma and 65 percent additive solution (as). because plt additive solutions (ass) are not used in the united states, this study evaluated the ability of the pct process to inactivate low levels of bacteria in pooled whole blood-derived plts (rdp) suspended in 100 percent plasma. | 2007 | 17880617 |
| identification of culturable stream water bacteria from urban, agricultural, and forested watersheds using 16s rrna gene sequencing. | bacteria present in water samples taken on a weekly basis, from june 2004 through june 2005, from three streams, were cultured on coliscan easygel agar plates. colonies representative of a variety of colors and morphologies were subjected to amplification and sequencing of a 1000-1100 nt portion of the 16s rrna gene. a total of 528 colonies were sequenced; these categorized into 26 genera and 78 species. of 175 dark blue/purple colonies presumed to be e. coli, sequence analysis indicated that 45 ... | 2007 | 17878554 |
| polymyxin b for the treatment of multidrug-resistant pathogens: a critical review. | polymyxins have re-emerged in clinical practice owing to the dry antibiotic development pipeline and worldwide increasing prevalence of nosocomial infections caused by multidrug-resistant (mdr) gram-negative bacteria. polymyxin b and colistin (polymyxin e) have been ultimately considered as the last-resort treatment of such infections. microbiological, pharmacokinetic, pharmacodynamic and clinical data available for polymyxin b are reviewed in this paper. polymyxin b has rapid in vitro bacterici ... | 2007 | 17878146 |
| the continuing challenge of esbls. | since their first description more than 20 years ago, escherichia coli and klebsiella pneumoniae possessing extended-spectrum class a beta-lactamases (esbls) continue to thwart our best clinical efforts. in the 'early years' the most common beta-lactamases were of the tem and shv varieties. now, ctx-m enzymes are being discovered throughout the world and are becoming the most prevalent beta-lactamases found in clinical isolates. the k. pneumoniae carbapenemases (kpc) (esbl-type enzymes that conf ... | 2007 | 17875405 |
| characterization of klebsiella pneumoniae isolates from new zealand sea lion pups. | 2007 | 17875370 | |
| phenolic compounds and antimicrobial activity of olive (olea europaea l. cv. cobrançosa) leaves. | we report the determination of phenolic compounds in olive leaves by reversed-phase hplc/dad, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely gram positive (bacillus cereus, b. subtilis and staphylococcus aureus), gram negative bacteria (pseudomonas aeruginosa, escherichia coli and klebsiella pneumoniae) and fungi (candida albicans and cryptococcus neoformans). seven phenolic compo ... | 2007 | 17873849 |
| pyogenic liver abscesses: mortality-related factors. | to analyse the characteristics and mortality-related factors in a series of patients hospitalized for pyogenic liver abscess (pla). | 2007 | 17873608 |
| molecular identification of coliform bacteria isolated from drinking water reservoirs with traditional methods and the colilert-18 system. | the accuracy of a traditional method (lactose utilization with acid and gas production) for the detection of coliform bacteria and e. coli was tested in comparison with method iso 9308-1 (based on acid formation from lactose) and the colilert-18 system (detection of beta-galactosidase). a total of 345 isolates were identified after isolation from water samples using api 20e strips. the colilert-18 led to the highest number of positive findings (95% of the isolates were assigned to coliforms), wh ... | 2008 | 17870668 |
| bacterial colonization in suspected sexually abused children. | previous studies concluded that symptomatic prepubertal children with gardnerella vaginalis infection should be investigated for child sexual abuse. gardnerella vaginalis is only one out of a group of organism that cause bacterial vaginosis. the aim of the study was to report the frequency of bacterial colonization as well as sexually transmitted diseases and to correlate these data with patient characteristics. | 2007 | 17868895 |
| [the antibacterial activity of fluoroquinolones against gram-negative bacteria isolated from gifu prefecture (2005)]. | we investigated the susceptibility to 6 fluoroquinolones against 433 strains of gram-negative bacteria isolated from 6 medical facilities in gifu prefecture between january and september in 2005, determined by the agar dilution methods in according with the japan society of chemotherapy. we also investigated the correlation between the degree of resistance to fluoroquinolones and the amino acid substitutions in the quinolone resistance-determining region (qrdr). the tested clinical isolates were ... | 2007 | 17867488 |
| resistance patterns of urinary isolates in a tertiary indian hospital. | to analyze the pathogenic organisms recovered from patients with urinary tract infection in a tertiary indian hospital setting along with determination of the occurrence and antimicrobial sensitivity of uropathogens on a retrospective basis during a period of one year. | 2007 | 17867478 |
| attenuating burn wound inflammation improves pulmonary function and survival in a burn-pneumonia model. | we previously showed that topical inhibition of inflammatory signaling in burn wounds reduced systemic inflammatory response and burn-induced pulmonary inflammation. we hypothesized that this topical intervention would attenuate burn-induced lung injury, improve pulmonary function, protect lungs from bacterial invasion, and reduce mortality. | 2007 | 17855827 |
| antimicrobial susceptibility among organisms from the asia/pacific rim, europe and latin and north america collected as part of test and the in vitro activity of tigecycline. | to describe antimicrobial susceptibility among bacterial isolates associated with hospital infections collected from 266 centres in asia/pacific rim (n = 1,947), north america (n = 24,283), latin america (n = 1,957) and europe (n = 8,796). | 2007 | 17855724 |
| first detection of vim-1 type metallo-beta-lactamase in a multidrug-resistant klebsiella pneumoniae clinical isolate from turkey also producing the ctx-m-15 extended-spectrum beta-lactamase. | 2007 | 17855196 | |
| pylephlebitis and pyogenic liver abscesses: a complication of hemorrhoidal banding. | hemorrhoidal banding is a well-established and safe outpatient procedure. septic complications of hemorrhoidal banding are rare but can be fatal. the first case of pylephlebitis (septic portal vein thrombosis) and pyogenic liver abscess following hemorrhoidal banding in a 49-year-old man with diabetes is reported in the present study. risk factors, management and the role of prophylaxis in immunocompromised patients are discussed. caution against hemorrhoidal banding in immunosuppressed patients ... | 2007 | 17853956 |
| clinical impact of infections caused by esbl-producing e. coli and k. pneumoniae. | the outcome of infections occurring at different anatomic sites caused by esbl-producing e. coli or k. pneumoniae was retrospectively analysed for a 3-y period. 23 cases were compared to 46 controls with infections caused by third-generation cephalosporin-susceptible strains matched by age, severity of illness and duration of hospitalization before onset of infection. only 27.8% of cases received appropriate empirical antimicrobial therapy compared with 79.6% of controls (or(paired)=0.053, p=0.0 ... | 2007 | 17852950 |
| predictive factors of septic shock and mortality in neutropenic patients. | neutropenia is a major risk factor for developing a serious infection. bacteremia still causes significant mortality among neutropenic patients with cancer. the purpose of this study was to identify risk factors for septic shock and for mortality in neutropenic patients with leukemia and bacteremia. consecutive samples from 20 patients with acute myeloid leukemia and bacteremia were studied during a 1 year period (january-december 2003). all patients received empirical antibiotic therapies for f ... | 2007 | 17852435 |
| gram-negative pathogen klebsiella oxytoca is associated with spontaneous chronic otitis media in toll-like receptor 4-deficient c3h/hej mice. | this report confirms the presence of gram-negative klebsiella bacteria in the middle ear of the c3h/hej mouse by culture, polymerase chain reaction (pcr), and electron microscopy. identification of the bacterial pathogen supports the c3h/hej mouse as an excellent model for spontaneous chronic otitis media and its effects on the middle and inner ear. | 2008 | 17851949 |
| complexes of 3d(n) metal ions with thiosemicarbazones: synthesis and antimicrobial activity. | the chelating behavior of the thiosemicarbazone derivatives of 2-hydroxy-8-r-tricyclo[7.3.1.0.(2,7)]tridecane-13-one (where r = h, ch3, c6h5) towards co(ii), ni(ii) and cu(ii) has been investigated by elemental analysis, molar conductivity measurements, uv-vis, ir, esr spectroscopy and thermal studies. it was deduced from the experiments performed that the ligands coordinate to metal ions in different ways--neutral bidentate or mononegative bidentate--depending on the nature of r. also, if metal ... | 2007 | 17851430 |
| emerging issues in the management of infections caused by multidrug-resistant gram-negative bacteria. | accumulating evidence indicates that treating seriously ill infected patients with active antibiotics early in the course of infection is critical to improving outcomes. the most common reason for ineffective empiric therapy is resistance to the agents used. gram-negative bacteria are becoming increasingly resistant to many commonly used antibiotics, and some cases require older, more toxic antibiotics for adequate microbial coverage. the diversity of resistance mechanisms that underly multidrug ... | 2007 | 17847174 |
| emergence of the quinolone resistance-mediating gene aac(6')-ib-cr in extended-spectrum-beta-lactamase-producing klebsiella isolates collected in slovenia between 2000 and 2005. | seventy-four nonrepetitive uropathogenic fluoroquinolone-resistant or -intermediate extended-spectrum-beta-lactamase-producing klebsiella isolates from slovenia were screened for the presence of plasmid-mediated quinolone resistance genes. none of the known qnr genes were detected. the aac(6')-ib-cr allele was detected on plasmids from 25 transconjugants for which the ciprofloxacin mic was higher than for the recipient escherichia coli strain. | 2007 | 17846136 |
| bacteriological profile of patients undergoing open heart surgery and evaluation of a bacterial filter using protected broncho-alveolar lavage. | twenty seven patients undergoing elective open heart surgery were included in this prospective study. they were randomly divided into two groups. group c (n = 12) constituted the control group in whom no breathing filter was used in the anaesthesia circuit in the operating room or in the icu. humidification of breathing gases was achieved with the help of conventional heated humidifier. in group f (n = 15), heat and moisture exahanging bacterial / viral filter was incorporated in the breathing c ... | 1998 | 17827620 |
| interplay between the temperate phages py54 and n15, linear plasmid prophages with covalently closed ends. | the objective of this study was to determine whether the temperate yersinia enterocolitica phage py54 may interact with the related escherichia coli phage n15 during both the lysogenic and the lytic cycle in the same cell. the py54 and n15 prophages are linear plasmids which have been shown to be compatible and stably replicating in e. coli and yersinia. in e. coli, the py54 prophage does not restrict n15 propagation. in contrast, n15 reduces by use of its cor gene the susceptibility of yersinia ... | 2007 | 17827299 |
| synthesis and structure-activity relationship of 7-(substituted)-aminomethyl-4-quinolone-3-carboxylic acid derivatives. | gram-positive organisms have re-emerged as the major hospital pathogens, which make the unmet medical needs for antibacterial therapy even worse. in searching for potent agents against gram-positive pathogens, novel 7-(substituted)-aminomethyl-quinolone-3-carboxylic acids were designed, synthesized, and evaluated for their antibacterial activities in vitro. many 7-monoarylaminomethyl derivatives exhibited high potency against gram-positive organisms compared to reference agents: vancomycin and p ... | 2007 | 17826097 |
| advances in defining etiology and new therapeutic approaches in acute diarrhea. | defining etiology of acute diarrhea is critical to disease therapy and prevention. in this review we look at recent developments in etiologic agents of acute diarrhea and advances in therapy and prevention of the illness. newly appreciated agents include enterotoxigenic bacteroides fragilis, klebsiella oxytoca and laribacter hongkongensis. atypical enteropathogenic e. coli (epec) strains lacking the gene for epithelial attachment appear to be more important as causes of diarrhea than traditional ... | 2007 | 17825422 |
| leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia. | the adipocyte-derived hormone leptin is an important regulator of appetite and energy expenditure and is now appreciated for its ability to control innate and adaptive immune responses. we have reported previously that the leptin-deficient ob/ob mouse exhibited increased susceptibility to the gram-negative bacterium klebsiella pneumoniae. in this report we assessed the impact of chronic leptin deficiency, using ob/ob mice, on pneumococcal pneumonia and examined whether restoring circulating lept ... | 2007 | 17822444 |
| a 60-year-old man with fever and a lung mass. | 2007 | 17806058 | |
| cloning and characterization of a 4-hydroxyphenylacetate 3-hydroxylase from the thermophile geobacillus sp. pa-9. | a 4-hydroxyphenylacetic acid (4-hpa) hydroxylase-encoding gene, on a 2.7-kb genomic dna fragment, was cloned from the thermophile geobacillus sp. pa-9. the geobacillus sp. pa-9 4-hpa hydroxylase gene, designated hpah, encodes a protein of 494 amino acids with a predicted molecular mass of 56.269 da. the deduced amino-acid sequence of the hpah gene product displayed <30% amino-acid sequence identity with the larger monooxygenase components of the previously characterized two-component 4-hpa 3-hyd ... | 2007 | 17805928 |
| susceptibility of klebsiella spp. to cefpirome and cefepime. | cefpirome and cefepime possess a greater antibacterial spectrum in vitro than third-generation cephalosporins because they are active against enterobacteriaceae, which produce beta-lactamases, which may inactivate third-generation cephalosporins. the aim of this study was to quantitatively compare the in vitro activity of cefpirome and cefepime against klebsiella spp. isolates. we have studied 342 klebsiella spp. clinical isolates, from some hospitals in eastern romania. minimum inhibitory conce ... | 2006 | 17802963 |
| herbicide resistance in transgenic plants expressing a bacterial detoxification gene. | the herbicide bromoxynil (3,5-dibromo-4-hydroxybenzonitrile) is a photosynthetic (photosystem ii) inhibitor in plants. a gene, bxn, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was cloned from the natural soil bacterium klebsiella ozaenae. for expression in plants, the bxn gene was placed under control of a light-regulated tissue-specific promoter, the ribulose bisphosphate carboxylase small subunit. transfer of this chimeric ... | 1988 | 17789813 |
| tlr9 is required for protective innate immunity in gram-negative bacterial pneumonia: role of dendritic cells. | in this study, experiments were performed to determine the contribution of tlr9 to the generation of protective innate immunity against virulent bacterial pathogens of the lung. in initial studies, we found that the intratracheal administration of klebsiella pneumoniae in wild-type (wt) balb/c mice resulted in the rapid accumulation of dendritic cells (dc) expressing tlr9. as compared with wt mice, animals deficient in tlr9 (tlr9-/-) displayed significantly increased mortality that was associate ... | 2007 | 17785831 |
| adverse hospital outcomes associated with the choice of empiric antibiotics in klebsiella pneumoniae pneumonia: a retrospective observational study. | in malaysia, klebsiella pneumoniae ranks high as a cause of adult pneumonia requiring hospitalisation. | 2007 | 17767334 |
| structure--activity study of the antibacterial peptide fallaxin. | fallaxin is a 25-mer antibacterial peptide amide, which was recently isolated from the west indian mountain chicken frog leptodactylus fallax. fallaxin has been shown to inhibit the growth of several gram-negative bacteria including enterobacter cloacae, escherichia coli, klebsiella pneumoniae, and pseudomonas aeruginosa. here, we report a structure-activity study of fallaxin based on 65 analogs, including a complete alanine scan and a full set of n- and c-terminal truncated analogs. the fallaxi ... | 2007 | 17766389 |
| old antibiotics for infections in critically ill patients. | the alarming epidemic of multidrug-resistant bacteria and the reluctance of the pharmaceutical industry to invest in the development of new antibiotics have forced clinicians to reintroduce forgotten antibiotics into their practice. this review highlights the effectiveness and safety of older antibiotics when used in the treatment of infections of critically ill patients. | 2007 | 17762241 |
| extended-spectrum beta-lactamases: will they be the new 'methicillin-resistant staphylococcus aureus'? | 2008 | 17760887 | |
| development of a surgical site infection (ssi) surveillance system, calculation of ssi rates and specification of important factors affecting ssi in a digestive organ surgical department. | we have developed an original system to conduct surgical site infection (ssi) surveillance. this system accumulates ssi surveillance information based on the national nosocomial infections surveillance (nnis) system and the japanese nosocomial infections surveillance (jnis) system. the features of this system are as follows: easy input of data, high generality, data accuracy, ssi rate by operative procedure and risk index category (ric) can be promptly calculated and compared with the current nn ... | 2007 | 17760267 |
| klebsiella pneumoniae respiratory isolates from 2000 to 2004 in a malaysian hospital: characteristics and relation to hospital antibiotics consumption. | klebsiella pneumoniae ranks high as a cause of community-acquired pneumonia in hospitalised patients in malaysia. | 2007 | 17728961 |
| community-acquired versus nosocomial klebsiella pneumonia bacteremia: clinical features, treatment outcomes, and clinical implication of antimicrobial resistance. | 2007 | 17728528 | |
| microbiological quality of indoor and outdoor swimming pools in greece: investigation of the antibiotic resistance of the bacterial isolates. | during 1997-2005, the microbiological quality and susceptibility of bacterial isolates of swimming pool waters were investigated. a total of 462 water samples were collected from three indoor swimming pools (a teaching pool, a competition public pool, a hydrotherapy pool) and two outdoor swimming pools (a hotel semi-public and a residential private pool) in northwestern greece. all water samples were analyzed for the presence of bacteria, protozoa and fungi and susceptibility tests were performe ... | 2008 | 17728184 |
| [diagnostic image (334). a man with a skin wound under the eye]. | a 44-year-old man with a facial skin wound after working with a motor lawn mower had an orbital and ocular penetration by a 2.4 cm metal wire, with endophthalmitis caused by a klebsiella ozaenae. | 2007 | 17725256 |
| first report of the emergence of ctx-m-type extended-spectrum beta-lactamases (esbls) as the predominant esbl isolated in a u.s. health care system. | ctx-m-type extended-spectrum beta-lactamases (esbls) have become increasingly common worldwide, with the notable exception of the united states, where tem- and shv-type esbls have appeared to predominate. we have noted the emergence of esbls in our health care system (the university health system in san antonio, tx), especially in escherichia coli isolates, that preferentially hydrolyze cefotaxime rather than ceftazidime, suggesting the possibility of ctx-m-type enzymes. microbiology laboratory ... | 2007 | 17724160 |
| crystal structure of cefditoren complexed with streptococcus pneumoniae penicillin-binding protein 2x: structural basis for its high antimicrobial activity. | cefditoren is the active form of cefditoren pivoxil, an oral cephalosporin antibiotic used for the treatment of respiratory tract infections and otitis media caused by bacteria such as streptococcus pneumoniae, haemophilus influenzae, streptococcus pyogenes, klebsiella pneumoniae, and methicillin-susceptible strains of staphylococcus aureus. beta-lactam antibiotics, including cefditoren, target penicillin-binding proteins (pbps), which are membrane-associated enzymes that play essential roles in ... | 2007 | 17724158 |
| plasmid determined resistance to quinolones in clinical isolates of gram-negative bacilli. | a total of 166 strains of gram-negative bacilli comprising 65 klebsiella species, 22 escherichia coli, 39 pseudomonas aeruginosa, 20 proteus species, 18 other pseudomonas species and 2 salmonella typhi, were isolated from clinical specimens in the university college hospital, ibadan. antimicrobial susceptibility testing and plasmid profiling of the strains were done. ceftazidine had the highest antibacterial activity of 83.1% compared to the fluoroquinolones followed by ciprofloxacin (78.3%), pe ... | 2006 | 17722809 |
| risk factors of nosocomial infection with extended-spectrum beta-lactamase-producing bacteria in a neonatal intensive care unit in china. | to study risk factors of neonatal nosocomial infection caused by extended-spectrum beta-lactamase (esbl)-producing bacteria in a neonatal intensive care unit (nicu). | 2007 | 17721736 |
| antimicrobial resistance of klebsiella pneumoniae in a saudi arabian hospital: results of a 6-year surveillance study, 1998-2003. | there are few data regarding the prevalence and trends of antibiotic resistance to klebsiella pneumoniae in saudi arabia over time. thus, we evaluated the prevalence and the trends in antibiotic resistance of k. pneumoniae at the saudi aramco medical services organization over a 6-year period. this was a retrospective study of the in vitro pattern and trends of antibiotic resistance of k. pneumoniae from 1998 to 2003. a total of 3070 distinct isolates of k. pneumoniae were analyzed. hospital-acq ... | 2007 | 17721686 |
| complex regulation of urease formation from the two promoters of the ure operon of klebsiella pneumoniae. | klebsiella pneumoniae can use urea as the sole source of nitrogen, thanks to a urease encoded by the uredabcefg operon. expression of this operon is independent of urea and is regulated by the supply of nitrogen in the growth medium. when cells were growth rate limited for nitrogen, the specific activity of urease was about 70 times higher than that in cells grown under conditions of excess nitrogen. much of this nitrogen regulation of urease formation depended on the nitrogen regulatory system ... | 2007 | 17720785 |
| microbial pathogens of public health significance in waste dumps and common sites. | microbial pathogens of public health significance found in waste and common sites were collected from four different dumping sites and assessed for pathogenic agents. the modified methods employed were based on the classical methods and basic principles of the reactions followed by biochemical enzymatic standards described for gram negative non fermenting bacteria. the results have shown presence of bacterial species including pseudomonas, mirococcus, actinomyces, neisseria, bacillus and klebsie ... | 2007 | 17718005 |
| diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates. | the diversity of fungi, bacteria, yeast, actinomycetes and protozoa were analysed in the gut and casts of eudrilus eugeniae, lampito mauritii, eisenia fetida and perionyx excavatus, both qualitatively and quantitatively as influenced by different feed substrates like clay loam soil, cowdung and pressmud. while actinomycetes (streptomyces albus, s. somaliensis, nocardia asteroides, n. caviae and saccharomonosporia) were not digested by any of these species of worms, protozoa (amoeba proteus, a. t ... | 2007 | 17717992 |
| postcollection synthesis of ethyl glucuronide by bacteria in urine may cause false identification of alcohol consumption. | ethyl glucuronide (etg) is a minor ethanol metabolite used as a specific marker to document recent alcohol consumption; confirm abstinence in treatment programs, workplaces, and schools; and provide legal proof of drinking. this study examined if bacterial pathogens in urine may enable postsampling synthesis of etg and ethyl sulfate (ets) from ethanol, leading to clinical false-positive results. | 2007 | 17717128 |
| the changing face of klebsiella pneumoniae infections in the community. | klebsiella pneumoniae (kp) is an important pathogen both in the community and the hospital setting. in the community the emergence of virulent organisms with predominantly k1/k2 capsular serotypes has been observed over the last 20 years, these pathogens cause a distinct clinical syndrome consisting of pyogenic liver abscesses, sometimes accompanied by meningitis and abscesses elsewhere. in the hospital environment, under heavy antibiotic usage, multiple drug resistance has been increasingly obs ... | 2007 | 17716872 |
| characterization and identification of virulent klebsiella oxytoca isolated from abalone (haliotis diversicolor supertexta) postlarvae with mass mortality in fujian, china. | an epidemic of mass mortality of abalone (haliotis diversicolor supertexta) postlarvae aged 40 days or less has existed across south coast of china since the second half of 2002. among 20 bacterial strains isolated from diseased abalone postlarvae on 2216e marine agar plates during an outbreak of postlarval disease in august 2005, a predominant strain (designated strain 20) was demonstrated to be virulent to postlarvae with an ld(50) value of 1.0x10(5) colony forming units (cfuml(-1)) on day 4, ... | 2008 | 17716687 |
| investigation of sources of potential bias in laboratory surveillance for anti-microbial resistance. | there are a number of biases that may influence the validity of laboratory-based surveillance for antimicrobial resistance. our objective was to evaluate the potential magnitude of bias in reporting of etiologic agents and their resistance rates associated with inclusion of multiple patient samples and non-random timing and location of sampling. | 2007 | 17716594 |
| antimicrobial resistance among gram-negative bacilli causing infections in intensive care unit patients in the united states between 1993 and 2004. | during the 12-year period from 1993 to 2004, antimicrobial susceptibility profiles of 74,394 gram-negative bacillus isolates recovered from intensive care unit (icu) patients in united states hospitals were determined by participating hospitals and collected in a central location. mics for 12 different agents were determined using a standardized broth microdilution method. the 11 organisms most frequently isolated were pseudomonas aeruginosa (22.2%), escherichia coli (18.8%), klebsiella pneumoni ... | 2007 | 17715376 |
| metabolic flux and robustness analysis of glycerol metabolism in klebsiella pneumoniae. | the knowledge of the mechanism of flux distribution will benefit understanding cell physiology and regulation of metabolism. in this study, the measured fluxes obtained under steady-state conditions were used to estimate intracellular fluxes and identify the robustness of branch points of the anaerobic glycerol metabolism in klebsiella pneumoniae for the production of 1,3-propanediol by metabolic flux analysis. the biomass concentration increased as nadh(2)/nad(+) decreased at low initial concen ... | 2008 | 17713793 |
| thermodynamics of ni2+, cu2+, and zn2+ binding to the urease metallochaperone uree. | the two ni2+ ions in the urease active site are delivered by the metallochaperone uree, whose metal binding properties are central to the assembly of this metallocenter. isothermal titration calorimetry (itc) has been used to quantify the stoichiometry, affinity, and thermodynamics of ni2+, cu2+, and zn2+ binding to the well-studied c-terminal truncated h144*uree from klebsiella aerogenes, ni2+ binding to the wild-type k. aerogenes uree protein, and ni2+ and zn2+ binding to the wild-type uree pr ... | 2007 | 17711301 |