Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| salmonella enteritidis in shell eggs: evolving concerns and innovative control measures. | the issue of egg contamination with salmonella enterica serovar enteritidis rose to prominence several decades ago with increasing rate of infection around the world. recent outbreaks have assured that this problem maintains a place in the public consciousness. extensive research has been conducted to investigate the factors precipitating contamination events, their avoidance, and mitigation of the threat of contaminated eggs; consequently, regulations have been put in place to increase the safe ... | 2012 | 22958532 |
| tigecycline challenge triggers srna production in salmonella enterica serovar typhimurium. | bacteria employ complex transcriptional networks involving multiple genes in response to stress, which is not limited to gene and protein networks but now includes small rnas (srnas). these regulatory rna molecules are increasingly shown to be able to initiate regulatory cascades and modulate the expression of multiple genes that are involved in or required for survival under environmental challenge. despite mounting evidence for the importance of srnas in stress response, their role upon antibi ... | 2012 | 22958399 |
| a novel salmonella serovar isolated from peregrine falcon (falco peregrinus) nestlings in sweden: salmonella enterica enterica serovar pajala (salmonella pajala). | a novel salmonella serovar was isolated from peregrine falcon (falco peregrinus) nestlings in northern sweden in 2006. three isolates of the same clone was retrieved from three falcon siblings and characterized as salmonella enterica sub-species enterica: o-phase 13, 23:-: e, n, z 15 and the h-phase was not present. we propose the geographical name salmonella enterica, sub-species enterica serovar pajala to this novel salmonella. | 2012 | 22957136 |
| prevalence of salmonella cerro in laboratory-based submissions of cattle and comparison with human infections in pennsylvania, 2005-2010. | the aim of this study was to identify salmonella serotypes infecting cattle in pennsylvania, to compare infection rates for the predominant serotype, salmonella enterica serotype cerro, with the infection rates for the same serotype in humans, and to study the clonal diversity and antimicrobial resistance for this serotype in cattle from 2005 to 2010. clonal diversity among the selected isolates was studied using pulsed-field gel electrophoresis (pfge) and repetitive (rep)-polymerase chain react ... | 2012 | 22953739 |
| regulation of rama by ramr in salmonella enterica serovar typhimurium: isolation of a ramr superrepressor. | rama is a transcription factor involved in regulating multidrug resistance in salmonella enterica serovar typhimurium sl1344. green fluorescent protein (gfp) reporter fusions were exploited to investigate the regulation of rama expression by ramr. we show that ramr represses the rama promoter by binding to a palindromic sequence and describe a superrepressor ramr mutant that binds to the rama promoter sequence more efficiently, thus exhibiting a rama inactivated phenotype. | 2012 | 22948865 |
| filter-based pathogen enrichment technology for detection of multiple viable foodborne pathogens in 1 day. | conventional foodborne pathogen assays currently used in the food industry often require long culture enrichments to increase pathogen levels so they can be detected. even using sensitive real-time pcr assays, culture enrichment at least overnight is necessary especially for detection of pathogens with slow growth rates such as listeria monocytogenes. to eliminate this cumbersome enrichment step and detect minute amounts of pathogens within 1 day, filter-based pathogen enrichment technology was ... | 2012 | 22947467 |
| longitudinal dissemination of salmonella enterica clonal groups through the slaughter process of salmonella-positive pig batches. | this study was conducted to assess the dissemination of salmonella clonal groups in slaughterhouses that received batches of salmonella -positive pigs and used different routine processing procedures. eight serial sampling sessions were conducted in three slaughterhouses (a, b, and c). blood was collected randomly (n = 25) from each batch of pigs and processed for serology. carcasses (n = 12) were identified and sampled after dehairing, after singeing, after evisceration, and before chilling. a ... | 2012 | 22947464 |
| increasing quinolone resistance and multidrug resistant isolates among salmonella enterica in hong kong. | to study the antimicrobial susceptibility and molecular epidemiology of salmonella enterica isolates from 2005 to 2010 in hong kong. | 2012 | 22947244 |
| comparative proteome and transcriptome analyses of wild-type and live vaccine strains of salmonella enterica serovar gallinarum. | salmonella enterica serovar gallinarum causes fowl typhoid in chickens and is of economic importance to the chicken industry. a serovar gallinarum live vaccine strain 9r (sg 9r) has been used to control fowl typhoid in many areas where the disease is endemic. because the attenuation mechanism of sg 9r was not defined, there has been continued concern about reversion to virulence. in this study, we examined the molecular characteristics, which might provide better insight into attenuation of sg 9 ... | 2012 | 22944630 |
| a dual tag system for facilitated detection of surface expressed proteins in escherichia coli. | the discovery of the autotransporter family has provided a mechanism for surface expression of proteins in laboratory strains of escherichia coli. we have previously reported the use of the aida-i autotransport system to express the salmonella enterica serovar enteritidis proteins sefa and h:gm. the sefa protein was successfully exposed to the medium, but the orientation of h:gm in the outer membrane could not be determined due to proteolytic cleavage of the n-terminal detection-tag. the goal of ... | 2012 | 22943700 |
| global transcriptional analysis of dehydrated salmonella enterica serovar typhimurium. | despite the scientific and industrial importance of desiccation tolerance in salmonella, knowledge regarding its genetic basis is still scarce. in the present study, we performed a transcriptomic analysis of dehydrated and water-suspended salmonella enterica serovar typhimurium using microarrays. dehydration induced expression of 90 genes and downregulated that of 7 genes. ribosomal structural genes represented the most abundant functional group with a relatively higher transcription during dehy ... | 2012 | 22941081 |
| prevalence and relative risk of cronobacter spp., salmonella spp., and listeria monocytogenes associated with the body surfaces and guts of individual filth flies. | although flies are important vectors of food-borne pathogens, there is little information to accurately assess the food-related health risk of the presence of individual flies, especially in urban areas. this study quantifies the prevalence and the relative risk of food-borne pathogens associated with the body surfaces and guts of individual wild flies. one hundred flies were collected from the dumpsters of 10 randomly selected urban restaurants. flies were identified using taxonomic keys before ... | 2012 | 22941079 |
| residual viral and bacterial contamination of surfaces after cleaning and disinfection. | environmental surfaces contaminated with pathogens can be sources of indirect transmission, and cleaning and disinfection are common interventions focused on reducing contamination levels. we determined the efficacy of cleaning and disinfection procedures for reducing contamination by noroviruses, rotavirus, poliovirus, parechovirus, adenovirus, influenza virus, staphylococcus aureus, and salmonella enterica from artificially contaminated stainless steel surfaces. after a single wipe with water, ... | 2012 | 22941071 |
| non-typhoidal salmonella encephalopathy involving lipopolysaccharide in cattle. | this study assessed the involvement of lipopolysaccharide (lps) in the non-typhoidal salmonella encephalopathy (ntse) caused by a unique isolate of salmonella enterica serovar saint-paul (sstpnpg). ntse was prevented by genetic (deletion of mure) or pharmacologic (polymyxin) disruption of lps on sstpnpg although the disruption of lps did not deter brain penetration of the strain. this is the first study to demonstrate that lps is involved in the manifestations of ntse. | 2013 | 22939987 |
| [model of chronic salmonellosis: parameters of infection and immune response in inbred mice genetically variable in susceptibility to salmonellosis]. | study parameters of chronic infection and immune response in i/st and a/sn line mice in the model of per oral infection of mice with salmonella enterica serovar typhimurium. | 2013 | 22937698 |
| multiplex t-rflp allows for increased target number and specificity: detection of salmonella enterica and six species of listeria in a single test. | a multiplex t-rflp test was developed to detect and identify salmonella enterica and all six species of listeria inoculated into milk at minimal levels. extensive in silico analysis was used to design a fifteen-primer, six-amplimer methodology and in vitro application showed target organism dna, when amplified individually, yielded the predicted terminal restriction fragments (trfs) following digestion. non-target organisms were either not-amplified or yielded trfs which did not interfere with t ... | 2012 | 22937073 |
| characterization and differential gene expression between two phenotypic phase variants in salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium strain 798 has previously been shown to undergo phenotypic phase variation. one of the phenotypes expresses virulence traits such as adhesion, while the other phenotype does not. phenotypic phase variation appears to correlate with the ability of this strain to cause persistent, asymptomatic infections of swine. a new method to detect cells in either phenotypic phase was developed using evans blue-uranine agar plates. using this new assay, rates of phenoty ... | 2012 | 22937065 |
| antibacterial effect of phosphates and polyphosphates with different chain length. | the aim of this study was to monitor the antibacterial effect of seven phosphate salts on selected strains of gram-negative and gram-positive bacteria, which could be considered responsible for food-borne diseases (bacillus cereus, bacillus subtilis, enterococcus faecalis, micrococcus luteus, staphylococcus aureus, citrobacter freundii, escherichia coli, proteus mirabilis, salmonella enterica ser. enteritidis and pseudomonas aeruginosa). for these purposes, phosphates differing in chain length w ... | 2012 | 22934995 |
| draft genome sequences of eight salmonella enterica serotype newport strains from diverse hosts and locations. | salmonellosis is a major contributor to the global public health burden. salmonella enterica serotype newport has ranked among three salmonella serotypes most commonly associated with food-borne outbreaks in the united states. it was thought to be polyphyletic and composed of independent lineages. here we report draft genomes of eight strains of s. newport from diverse hosts and locations. | 2012 | 22933769 |
| insights from the genome sequence of a salmonella enterica serovar typhi strain associated with a sporadic case of typhoid fever in malaysia. | salmonella enterica serovar typhi is the causative agent of typhoid fever, which causes nearly 21.7 million illnesses and 217,000 deaths globally. herein, we describe the whole-genome sequence of the salmonella typhi strain st0208, isolated from a sporadic case of typhoid fever in kuala lumpur, malaysia. the whole-genome sequence and comparative genomics allow an in-depth understanding of the genetic diversity, and its link to pathogenicity and evolutionary dynamics, of this highly clonal pathog ... | 2012 | 22933756 |
| whole-genome sequences and comparative genomics of salmonella enterica serovar typhi isolates from patients with fatal and nonfatal typhoid fever in papua new guinea. | many of the developing countries of the southeast asian region are significantly affected by endemic typhoid fever, possibly as a result of marginal living standards. it is an important public health problem in countries such as papua new guinea, which is geographically close to some of the foci of endemicity in asia. the severity of the disease varies in different regions, and this may be attributable to genetic diversity among the native strains. genome sequence data on strains from different ... | 2012 | 22933755 |
| salmonella enterica serovar agbeni, british columbia, canada, 2011. | 2012 | 22932699 | |
| the dub-ious lack of alis in salmonella infection: a salmonella deubiquitinase regulates the autophagy of protein aggregates. | ubiquitinated aggregates are formed in eukaryotic cells in response to several external stimuli, including exposure to bacterial lipopolysaccharide (lps). although salmonella enterica serovar typhimurium (s. typhimurium) lps has been shown to induce aggresome-like induced structures (alis) in macrophages, these have not been described in s. typhimurium-infected macrophages. given that lps is present in infection, this suggests that s. typhimurium might suppress the formation of alis. we found th ... | 2012 | 22931829 |
| residual antibiotics disrupt meat fermentation and increase risk of infection. | fermented sausages, although presumed safe for consumption, sometimes cause serious bacterial infections in humans that may be deadly. not much is known about why and when this is the case. we tested the hypothesis that residual veterinary antibiotics in meat can disrupt the fermentation process, giving pathogenic bacteria a chance to survive and multiply. we found that six commercially available starter cultures were susceptible to commonly used antibiotics, namely, oxytetracycline, penicillin, ... | 2012 | 22930338 |
| spontaneous and transient defence against bacteriophage by phase-variable glucosylation of o-antigen in salmonella enterica serovar typhimurium. | as natural killers of bacteria, bacteriophages have forced bacteria to develop a variety of defence mechanisms. the alteration of host receptors is one of the most common bacterial defence strategies against phage infection, which completely blocks phage attachment but comes at a potential fitness cost to the bacteria. here, we report the cost-free, transient emergence of phage resistance in salmonella enterica subspecies enterica serovar typhimurium through a phase-variable modification of the ... | 2012 | 22928771 |
| receptor diversity and host interaction of bacteriophages infecting salmonella enterica serovar typhimurium. | salmonella enterica subspecies enterica serovar typhimurium is a gram-negative pathogen causing salmonellosis. salmonella typhimurium-targeting bacteriophages have been proposed as an alternative biocontrol agent to antibiotics. to further understand infection and interaction mechanisms between the host strains and the bacteriophages, the receptor diversity of these phages needs to be elucidated. | 2012 | 22927964 |
| interactions between the termini of lumen enzymes and shell proteins mediate enzyme encapsulation into bacterial microcompartments. | bacterial microcompartments (mcps) are a widespread family of proteinaceous organelles that consist of metabolic enzymes encapsulated within a protein shell. for mcps to function specific enzymes must be encapsulated. we recently reported that a short n-terminal targeting sequence of propionaldehyde dehydrogenase (pdup) is necessary and sufficient for the packaging of enzymes into a mcp that functions in 1,2-propanediol (1,2-pd) utilization (pdu) by salmonella enterica. here we show that encapsu ... | 2012 | 22927404 |
| cathelicidin antimicrobial peptide expression is not induced or required for bacterial clearance during salmonella enterica infection of human monocyte-derived macrophages. | salmonella enterica serovar typhimurium is able to resist antimicrobial peptide killing by induction of the phop-phoq and pmra-pmrb two-component systems and the lipopolysaccharide (lps) modifications they mediate. murine cathelin-related antimicrobial peptide (cramp) has been reported to inhibit s. typhimurium growth in vitro and in vivo. we hypothesize that infection of human monocyte-derived macrophages (mdms) with salmonella enterica serovar typhi and s. typhimurium will induce human catheli ... | 2012 | 22927052 |
| review of pathogenesis and diagnostic methods of immediate relevance for epidemiology and control of salmonella dublin in cattle. | salmonella enterica subsp. enterica serovar dublin (s. dublin) receives increasing attention in cattle production. it is host-adapted to cattle, and leads to unacceptable levels of morbidity, mortality and production losses in both newly and persistently infected herds. cattle health promoting institutions in several countries are currently constructing active surveillance programmes or voluntary certification programmes, and encourage control and eradication of s. dublin infected cattle herds. ... | 2013 | 22925272 |
| type i interferon induces necroptosis in macrophages during infection with salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium (s. typhimurium) is a virulent pathogen that induces rapid host death. here we observed that host survival after infection with s. typhimurium was enhanced in the absence of type i interferon signaling, with improved survival of mice deficient in the receptor for type i interferons (ifnar1(-/-) mice) that was attributed to macrophages. although there was no impairment in cytokine expression or inflammasome activation in ifnar1(-/-) macrophages, they were h ... | 2012 | 22922364 |
| [salmonella multiphasic flagellar antigen]. | in the salmonella antigenic pattern, more than one phase of flagellar antigen is observed. the phase of flagellar antigen depends of the gene which encodes the protein building the filament of flagella. the flic gene encodes the 1st phase of flagellar antigen and the fljb gene encodes the 2nd phase of flagellar antigen. the third phase of flagellar antigen is encoded by one of the genes localized on the plasmid. expression of the fljb gene (part of the hinfljba operon) is regulated by a mechanis ... | 2012 | 22922144 |
| reciprocal control between a bacterium's regulatory system and the modification status of its lipopolysaccharide. | gram-negative bacteria often modify their lipopolysaccharide (lps), thereby increasing resistance to antimicrobial agents and avoidance of the host immune system. however, it is unclear how bacteria adjust the levels and activities of lps-modifying enzymes in response to the modification status of their lps. we now address this question by investigating the major regulator of lps modifications in salmonella enterica. we report that the pmra/pmrb system controls expression of a membrane peptide t ... | 2012 | 22921935 |
| escherichia coli strongly supports the growth of histomonas meleagridis, in a monoxenic culture, without influence on its pathogenicity. | based on clonal cultures of histomonas meleagridis, monoxenic cultures have, to our knowledge for the first time, been established in a liquid medium. the faecal flora was exchanged for defined bacterial strains by selective destruction of the initial bacteria with a variety of antibiotics, keeping the flagellate alive. the growth of the protozoan parasite was found to depend on the bacteria, especially on their energy metabolism. escherichia coli was found to strongly support the growth of the ... | 2012 | 22921600 |
| the effect of low shear force on the virulence potential of yersinia pestis: new aspects that space-like growth conditions and the final frontier can teach us about a formidable pathogen. | manned space exploration has created a need to evaluate the effects of space-like stress (sls) on pathogenic and opportunistic microbes. interestingly, several gram-negative enteric pathogens, e.g., salmonella enterica serovar typhimurium, have revealed a transient hyper-virulent phenotype following simulated microgravity (smg) or actual space flight exposures. we have explored the virulence potential of yersinia pestis kim/d27 (yp) following exposure to mechanical low shear forces associated wi ... | 2012 | 22919696 |
| cationic antimicrobial peptides serve as activation signals for the salmonella typhimurium phopq and pmrab regulons in vitro and in vivo. | salmonella enterica serovar typhimurium uses two-component regulatory systems (tcrss) to respond to environmental stimuli. upon infection, the tcrss phop-phoq (phopq) and pmra-pmrb (pmrab) are activated by environmental signals detected in the lumen of the intestine and within host cells. tcrs-mediated gene expression leads to upregulation of genes involved in lipopolysaccharide (lps) modification and cationic antimicrobial peptide (camp) resistance. this research expands on previous studies whi ... | 2012 | 22919691 |
| extreme antimicrobial peptide and polymyxin b resistance in the genus burkholderia. | cationic antimicrobial peptides and polymyxins are a group of naturally occurring antibiotics that can also possess immunomodulatory activities. they are considered a new source of antibiotics for treating infections by bacteria that are resistant to conventional antibiotics. members of the genus burkholderia, which includes various human pathogens, are inherently resistant to antimicrobial peptides. the resistance is several orders of magnitude higher than that of other gram-negative bacteria s ... | 2011 | 22919572 |
| dbdiarrhea: the database of pathogen proteins and vaccine antigens from diarrheal pathogens. | diarrhea occurs world-wide and is most commonly caused by gastrointestinal infections which kill around 2.2 million people globally each year, mostly children in developing countries. we describe here dbdiarrhea, which is currently the most comprehensive catalog of proteins implicated in the pathogenesis of diarrhea caused by major bacterial, viral and parasitic species. the current release of the database houses 820 proteins gleaned through an extensive and critical survey of research articles ... | 2012 | 22917656 |
| rates of gyrase supercoiling and transcription elongation control supercoil density in a bacterial chromosome. | gyrase catalyzes negative supercoiling of dna in an atp-dependent reaction that helps condense bacterial chromosomes into a compact interwound "nucleoid." the supercoil density (σ) of prokaryotic dna occurs in two forms. diffusible supercoil density (σ(d)) moves freely around the chromosome in 10 kb domains, and constrained supercoil density (σ(c)) results from binding abundant proteins that bend, loop, or unwind dna at many sites. diffusible and constrained supercoils contribute roughly equally ... | 2012 | 22916023 |
| polyfunctional cd4+ t cell responses to immunodominant epitopes correlate with disease activity of virulent salmonella. | salmonella enterica serovars are intracellular bacteria capable of causing typhoid fever and gastroenteritis of significant morbidity and mortality worldwide. current prophylactic and therapeutic treatment is hampered by the emergence of multidrug-resistant (mdr) strains of salmonella, and vaccines provide only temporal and partial protection in vaccinees. to develop more effective salmonella vaccines, it is important to understand the development of protective adaptive immunity to virulent salm ... | 2012 | 22912884 |
| oral administration of a combination of select lactic acid bacteria strains to reduce the salmonella invasion and inflammation of broiler chicks. | one-day-old chicks are susceptible to infection by strains of salmonella enterica subspecies. because multistrain probiotics are suggested to be more effective than monostrain probiotics due to the additive and synergistic effects, in this study, we prepared a multistrain formula a (mfa) consisting of 4 lactic acid bacteria (lab) strains selected by enhancing the tnf-α production for mouse macrophage 264.7 cells. the antagonistic effect of this mfa against the cecal colonization, viscera invasiv ... | 2012 | 22912447 |
| bacteria and protozoa differentially modulate the expression of rab proteins. | phagocytic cells represent an important line of innate defense against microorganisms. uptake of microorganisms by these cells involves the formation of a phagosome that matures by fusing with endocytic compartments, resulting in killing of the enclosed microbe. small gtpases of the rab family are key regulators of vesicular trafficking in the endocytic pathway. intracellular pathogens can interfere with the function of these proteins in order to subvert host immune responses. however, it is unk ... | 2012 | 22911692 |
| fis is essential for the stability of linear plasmid pbssb1 and affects the motility of salmonella enterica serovar typhi. | pbssb1 is a 27 kb non-bacteriophage-related linear plasmid first found in salmonella enterica serovar typhi (s. typhi), but the mechanism underlying the replication of pbssb1 is currently unknown. previous reports showed that the factor for inversion stimulation (fis) encoded by fis can affect the replication, transcription and other processes through binding dna. here, a fis deletion mutant of s. typhi (δfis) was prepared through the homologous recombination mediated by suicide plasmid and the ... | 2012 | 22911678 |
| functional analysis of the genes encoding diaminopropionate ammonia lyase in escherichia coli and salmonella enterica serovar typhimurium. | diaminopropionate ammonia lyase (dapal) is a pyridoxal-5'phosphate (plp)-dependent enzyme that catalyzes the conversion of diaminopropionate (dap) to pyruvate and ammonia and plays an important role in cell metabolism. we have investigated the role of the ygex gene of escherichia coli k-12 and its ortholog, stm1002, in salmonella enterica serovar typhimurium lt2, presumed to encode dapal, in the growth kinetics of the bacteria. while salmonella typhimurium lt2 could grow on dl-dap as a sole carb ... | 2012 | 22904288 |
| immediate after birth transmission of epidemic salmonella enterica typhimurium monophasic strains in pigs is a likely event. | 2012 | 22904242 | |
| prevalence, antimicrobial resistance and relation to indicator and pathogenic microorganisms of salmonella enterica isolated from surface waters within an agricultural landscape. | during a 12 month period (june 2007-may 2008), the prevalence and susceptibility of salmonella serovars and their relation to specific pathogenic and indicator bacteria in river and coastal waters was investigated. a total of 240 water samples were collected from selected sites in acheron and kalamas rivers and the ionian sea coast in north western greece. the samples were analyzed for salmonella spp., listeria spp., campylobacter spp., escherichia coli o157, staphylococci, pseudomonas spp., tot ... | 2013 | 22901425 |
| bacterial pathogens commandeer rab gtpases to establish intracellular niches. | intracellular bacterial pathogens deploy virulence factors termed effectors to inhibit degradation by host cells and to establish intracellular niches where growth and differentiation take place. here, we describe mechanisms by which human bacterial pathogens (including chlamydiae; coxiella burnetii; helicobacter pylori; legionella pneumophila; listeria monocytogenes; mycobacteria; pseudomonas aeruginosa, salmonella enterica) modulate endocytic and exocytic rab gtpases in order to thrive in host ... | 2012 | 22901006 |
| a comprehensive subcellular proteomic survey of salmonella grown under phagosome-mimicking versus standard laboratory conditions. | towards developing a systems-level pathobiological understanding of salmonella enterica, we performed a subcellular proteomic analysis of this pathogen grown under standard laboratory and phagosome-mimicking conditions in vitro. analysis of proteins from cytoplasmic, inner membrane, periplasmic, and outer membrane fractions yielded coverage of 25% of the theoretical proteome. confident subcellular location could be assigned to over 1000 proteins, with good agreement between experimentally observ ... | 2012 | 22900174 |
| suppressor analyses identify threonine as a modulator of rida mutant phenotypes in salmonella enterica. | the rida (yjgf/yer057c/uk114) family of proteins is broadly conserved in the three domains of life yet the functional understanding of these proteins is at an early stage. physiological studies of rida mutant strains of salmonella enterica provided a framework to inform in vitro studies and led to the description of a conserved biochemical activity for this family. rida mutant strains of s. enterica have characteristic phenotypes including new synthesis of thiamine biosynthetic intermediate phos ... | 2012 | 22900093 |
| caspase-11 increases susceptibility to salmonella infection in the absence of caspase-1. | inflammasomes are cytosolic multiprotein complexes assembled by intracellular nucleotide-binding oligomerization domain (nod)-like receptors (nlrs) and they initiate innate immune responses to invading pathogens and danger signals by activating caspase-1 (ref. 1). caspase-1 activation leads to the maturation and release of the pro-inflammatory cytokines interleukin (il)-1β and il-18, as well as lytic inflammatory cell death known as pyroptosis. recently, a new non-canonical inflammasome was desc ... | 2012 | 22895188 |
| [protection against salmonella via immunization with recombinant lactic acid bacteria]. | mucosal immunization of hens may be effective to prevent contamination of salmonella enterica serovar enteritidis (se) in eggs. lactic acid bacteria have been considered potential vaccine delivery agents because they are safe, immunogenic, and inexpensive. our research group has been investigating the development of oral vaccines against se using a lactobacillus casei strain as an antigen delivery vehicle. recombinant lactobacilli expressing se antigens flic and sipc have been constructed and ad ... | 2012 | 22894061 |
| salmonella "sops" up a preferred electron receptor in the inflamed intestine. | the microbiota of the mammalian intestinal tract represents a formidable barrier to colonization by pathogens. to overcome this resistance to colonization, bacterial pathogens use virulence factors to induce intestinal inflammation, which liberates nutrients for selective use by the infecting microbe. studies of salmonella enterica serovar typhimurium (s. typhimurium) infection in a streptomycin-treated mouse colitis model show how virulence factor-induced inflammation can produce nutrients used ... | 2012 | 22893385 |
| biofilm formation, cell surface hydrophobicity, and fatty acids analysis of starved salmonella enterica serovar typhimurium in seawater. | salmonella is an international foodborne pathogen widely disseminated in seawater that regularly causes large outbreaks of food poisoning. in this study, we have investigated the effect of starvation on the ability of salmonella enterica serovar typhimurium cells to adhere to polystyrene microplate and hep2 cells in seawater microcosms after incubation for 3 years. cell surface hydrophobicity was evaluated. effect of stress on fatty acids composition was also established. our results showed that ... | 2012 | 22891961 |
| differential modulation of intracellular survival of cytosolic and vacuolar pathogens by curcumin. | curcumin, a principal component of turmeric, acts as an immunomodulator regulating the host defenses in response to a diseased condition. the role of curcumin in controlling certain infectious diseases is highly controversial. it is known to alleviate symptoms of helicobacter pylori infection and exacerbate that of leishmania infection. we have evaluated the role of curcumin in modulating the fate of various intracellular bacterial pathogens. we show that pretreatment of macrophages with curcumi ... | 2012 | 22890770 |
| non-genetic diversity shapes infectious capacity and host resistance. | the spontaneous generation of distinct phenotypes within a clonal population of cells allows for both bet-hedging at the population level and the division of labor among subpopulations. this is emerging as an important theme in bacterial pathogenesis, because bacterial pathogens exhibit phenotypic heterogeneity with respect to characteristics that impact virulence. the phenomenon of persister cells and models of salmonella enterica serovar typhimurium (s. typhimurium) pathogenesis illustrate the ... | 2012 | 22889945 |
| investigation into the role of five salmonella enterica serovar enteritidis genomic islands in colonization of the chicken reproductive tract and other organs following oral challenge. | salmonella enterica serovar enteritidis is a major cause of human gastrointestinal tract disease, infection being due in large part to the consumption of contaminated eggs. recent genome sequencing of s. enterica serovars has identified genomic islands, the presence of which differs between serovars. using defined mutants, we have investigated the contribution that five such loci play in the colonization of the avian reproductive tract, other organs and avian macrophages. all loci appear to play ... | 2012 | 22889182 |
| low-shear modeled microgravity enhances salmonella enterica resistance to hydrogen peroxide through a mechanism involving katg and katn. | studies carried out in recent years have established that growth under conditions of reduced gravity enhances salmonella enterica serovar typhimurium virulence. to analyze the possibility that this microgravity-induced increase in pathogenicity could involve alterations in the ability of salmonella to withstand oxidative stress, we have compared the resistance to hydrogen peroxide of various salmonella enterica strains grown under conditions of low shear modeled microgravity (lsmmg) or normal gr ... | 2012 | 22888375 |
| evolution of a multiple antibiotic resistance region in inchi1 plasmids: reshaping resistance regions in situ. | to determine the structure of the resistance region in an inchi1 plasmid conferring resistance to multiple antibiotics, including gentamicin, recovered from a salmonella enterica serovar typhimurium isolate from a horse. | 2012 | 22888274 |
| bacterial infection of macrophages induces decrease in refractive index. | infection of cells by pathogens leads to both biochemical and structural modifications of the host cell. to study the structural modifications in a label-free manner, we use digital holographic microscopy, dhm, to obtain the integral refractive index distribution of cells. primary murine bone marrow derived macrophages (bmdm) infected with salmonella enterica serovar typhimurium, undergo highly significant reduction in refractive index, ri, compared to uninfected cells. infected bmdm cells from ... | 2013 | 22887897 |
| pathways of genetic adaptation: multistep origin of mutants under selection without induced mutagenesis in salmonella enterica. | in several bacterial systems, mutant cell populations plated on growth-restricting medium give rise to revertant colonies that accumulate over several days. one model suggests that nongrowing parent cells mutagenize their own genome and thereby create beneficial mutations (stress-induced mutagenesis). by this model, the first-order induction of new mutations in a nongrowing parent cell population leads to the delayed accumulation of visible colonies. in an alternative model (selection only), sel ... | 2012 | 22887815 |
| detection of salmonella enterica isolates producing ctx-m cephalosporinase in u.s. livestock populations. | we report the detection of salmonella carrying bla(ctx-m) in u.s. livestock populations. we identified 12 of 2,034 (0.6%) salmonella isolates originating from turkeys, horses, and pigs from at least 6 u.s. states, all carrying bla(ctx-m-1), many on a pandemic sequence type 1 incn plasmid. | 2012 | 22885753 |
| epidemiology of a salmonella enterica subsp. enterica serovar typhimurium strain associated with a songbird outbreak. | salmonella enterica subsp. enterica serovar typhimurium is responsible for the majority of salmonellosis cases worldwide. this salmonella serovar is also responsible for die-offs in songbird populations. in 2009, there was an s. typhimurium epizootic reported in pine siskins in the eastern united states. at the time, there was also a human outbreak with this serovar that was associated with contaminated peanuts. as peanuts are also used in wild-bird food, it was hypothesized that the pine siskin ... | 2012 | 22885752 |
| human infections attributable to the d-tartrate-fermenting variant of salmonella enterica serovar paratyphi b in germany originate in reptiles and, on rare occasions, poultry. | in this study, the population structure, incidence, and potential sources of human infection caused by the d-tartrate-fermenting variant of salmonella enterica serovar paratyphi b [s. paratyphi b (dt+)] was investigated. in germany, the serovar is frequently isolated from broilers. therefore, a selection of 108 epidemiologically unrelated s. enterica serovar paratyphi b (dt+) strains isolated in germany between 2002 and 2010 especially from humans, poultry/poultry meat, and reptiles was investig ... | 2012 | 22885742 |
| phosphorylation of nlrc4 is critical for inflammasome activation. | nlrc4 is a cytosolic member of the nod-like receptor family that is expressed in innate immune cells. it senses indirectly bacterial flagellin and type iii secretion systems, and responds by assembling an inflammasome complex that promotes caspase-1 activation and pyroptosis. here we use knock-in mice expressing nlrc4 with a carboxy-terminal 3×flag tag to identify phosphorylation of nlrc4 on a single, evolutionarily conserved residue, ser 533, following infection of macrophages with salmonella e ... | 2012 | 22885697 |
| emergence of fluoroquinolone resistance in salmonella enterica serovar typhi in andaman and nicobar islands, india. | 2012 | 22885270 | |
| electrochemical detection of salmonella using gold nanoparticles. | a disposable immunosensor for salmonella enterica subsp. enterica serovar typhimurium lt2 (s) detection using a magneto-immunoassay and gold nanoparticles (aunps) as label for electrochemical detection is developed. the immunosensor is based on the use of a screen-printed carbon electrode (spce) that incorporates a permanent magnet underneath. salmonella containing samples (i.e. skimmed milk) have been tested by using anti-salmonella magnetic beads (mbs-psab) as capture phase and sandwiching aft ... | 2013 | 22884647 |
| assessment of a regulatory sanitization process in egyptian dairy plants in regard to the adherence of some food-borne pathogens and their biofilms. | food-borne pathogens may develop certain strategies that enable them to defy harsh conditions such as chemical sanitization. biofilm formation represents a prominent one among those adopted strategies, by which food-borne pathogens protect themselves against external threats. thus, bacterial biofilm is considered as a major hazard for safe food production. this study was designed to investigate the adherence and the biofilm formation ability of some food-borne pathogens on stainless steel and po ... | 2012 | 22884171 |
| genome-wide detection of spontaneous chromosomal rearrangements in bacteria. | genome rearrangements have important effects on bacterial phenotypes and influence the evolution of bacterial genomes. conventional strategies for characterizing rearrangements in bacterial genomes rely on comparisons of sequenced genomes from related species. however, the spectra of spontaneous rearrangements in supposedly homogenous and clonal bacterial populations are still poorly characterized. here we used 454 pyrosequencing technology and a 'split mapping' computational method to identify ... | 2012 | 22880062 |
| antisense transcription is pervasive but rarely conserved in enteric bacteria. | noncoding rnas, including antisense rnas (asrnas) that originate from the complementary strand of protein-coding genes, are involved in the regulation of gene expression in all domains of life. recent application of deep-sequencing technologies has revealed that the transcription of asrnas occurs genome-wide in bacteria. although the role of the vast majority of asrnas remains unknown, it is often assumed that their presence implies important regulatory functions, similar to those of other nonco ... | 2012 | 22872780 |
| characterization of extended-spectrum beta-lactamase-producing salmonella enterica serotype brunei and heidelberg at the hussein dey hospital in algiers (algeria). | the purpose of this work was to study the genetic determinants responsible for extended-spectrum cephalosporin (esc) resistance of salmonella collected during the period of 1995-2008 at the hussein dey hospital in algiers (algeria). fourteen esc-resistant salmonella isolates were tested towards 22 antimicrobial agents. polymerase chain reaction (pcr) and sequencing were used to determine the underlying genetic determinants responsible for the extended-spectrum beta-lactamase (esbl) phenotypes. e ... | 2012 | 22871227 |
| farm-level associations with the shedding of salmonella and antimicrobial-resistant salmonella in u.s. dairy cattle. | salmonella enterica is the leading cause of foodborne-related deaths and hospitalizations within the united states. infections caused by antimicrobial-resistant (amr) strains are associated with higher hospital costs and case fatality. the objective for this study was to determine the association of management practices with the recovery of salmonella and amr salmonella on dairy herds. individual adult cow fecal samples and/or composite fecal samples were collected from 265 dairy herds in 17 sta ... | 2012 | 22870913 |
| the asd(+)-dadb(+) dual-plasmid system offers a novel means to deliver multiple protective antigens by a recombinant attenuated salmonella vaccine. | we developed means to deliver multiple heterologous antigens on dual plasmids with non-antibiotic-resistance markers in a single recombinant attenuated vaccine strain of salmonella enterica serotype typhimurium. the first component of this delivery system is a strain of s. typhimurium carrying genomic deletions in alr, dadb, and asd, resulting in obligate requirements for diaminopimelic acid (dap) and d-alanine for growth. the second component is the asd(+)-dadb(+) plasmid pair carrying wild-typ ... | 2012 | 22868499 |
| hemophagocytic macrophages in murine typhoid fever have an anti-inflammatory phenotype. | histiocytes are white blood cells of the monocytic lineage and include macrophages and dendritic cells. in patients with a variety of infectious and noninfectious inflammatory disorders, histiocytes can engulf nonapoptotic leukocytes and nonsenescent erythrocytes and thus become hemophagocytes. we report here the identification and characterization of splenic hemophagocytes in a natural model of murine typhoid fever. the development of a flow-cytometric method allowed us to identify hemophagocyt ... | 2012 | 22868497 |
| grapefruit juice and its constituents augment the effect of low ph on inhibition of survival and adherence to intestinal epithelial cells of salmonella enterica serovar typhimurium pt193. | the present study examined the survival of salmonella typhimurium and its adherence to intestinal epithelial cells following inoculation into grapefruit juice and apple cider. both liquids significantly inactivated s. typhimurium (0.8-2.2 log reduction compared to the control); surviving salmonella in grapefruit juice was 1.0-1.4 log lower than in apple cider at 24h incubation. grapefruit juice contains the antimicrobial substances naringin (nar) and naringenin (nge); however, the effect of grap ... | 2012 | 22867848 |
| reduced na+ affinity increases turnover of salmonella enterica serovar typhimurium melb. | the melibiose permease of salmonella enterica serovar typhimurium (melb(st)) catalyzes symport of melibiose with na(+), li(+), or h(+). bioinformatics and mutational analyses indicate that a conserved gly117 (helix iv) is a component of the na(+)-binding site. in this study, gly117 was mutated to ser, asn, or cys. all three mutations increase the maximum rate (v(max)) for melibiose transport in escherichia coli dw2 and greatly decrease na(+) affinity, indicating that intracellular release of na( ... | 2012 | 22865849 |
| multiple pathways of duplication formation with and without recombination (reca) in salmonella enterica. | duplications are often attributed to "unequal recombination" between separated, directly repeated sequence elements (>100 bp), events that leave a recombinant element at the duplication junction. however, in the bacterial chromosome, duplications form at high rates (10(-3)-10(-5)/cell/division) even without recombination (reca). here we describe 1800 spontaneous lac duplications trapped nonselectively on the low-copy f'(128) plasmid, where lac is flanked by direct repeats of the transposable ele ... | 2012 | 22865732 |
| high pressure treatments on the inactivation of salmonella enteritidis and the characteristics of beef carpaccio. | the effect of high pressure (hp) on salmonella enterica subsp. enterica serovar enteritidis in beef carpaccio stored under temperature abuse conditions (8 °c) during 30 days was investigated. after treatment, reductions of s. enteritidis were 3.68 and 5.94 log cfu/g in samples pressurized at 450 mpa for 5 and 10 min, respectively, whereas the pathogen was only detected after enrichment of samples treated at 450 mpa for 15 min. during storage, counts of s. enteritidis decreased 0.26 log cfu/g in ... | 2012 | 22863078 |
| a genomic island in salmonella enterica ssp. salamae provides new insights on the genealogy of the locus of enterocyte effacement. | the genomic island encoding the locus of enterocyte effacement (lee) is an important virulence factor of the human pathogenic escherichia coli. lee typically encodes a type iii secretion system (t3ss) and secreted effectors capable of forming attaching and effacing lesions. although prominent in the pathogenic e. coli such as serotype o157:h7, lee has also been detected in citrobacter rodentium, e. albertii, and although not confirmed, it is likely to also be in shigella boydii. previous phyloge ... | 2012 | 22860002 |
| cross-reactive gut-directed immune response against salmonella enterica serovar paratyphi a and b in typhoid fever and after oral ty21a typhoid vaccination. | there are no vaccines against paratyphoid fever in clinical use. the disease has become more wide-spread and there is a growing problem of antibiotic resistance among the strains. previous reports suggest that the oral live salmonella typhi ty21a-vaccine confers protection against paratyphoid b fever. data on efficacy against paratyphoid a fever are somewhat contentious. the present study investigated the immunological basis for such efficacy reports at a single-cell level: plasmablasts (identif ... | 2012 | 22858557 |
| tandem attenuators control expression of the salmonella mgtcbr virulence operon. | the mgtcbr operon from salmonella enterica serovar typhimurium specifies the virulence protein mgtc, the mg(2+) transporter mgtb and the regulatory peptide mgtr. the mgtcbr transcript includes a long leader region harbouring two short open reading frames (orfs). translation of these orfs is anticipated to impact the formation of particular stem-loop structures and control transcription of the coding region by an attenuation-like mechanism. we previously reported that orf mgtm enables salmonella ... | 2012 | 22857388 |
| efficacy of soluble recombinant flic protein from salmonella enterica serovar enteritidis as a potential vaccine candidate against homologous challenge in chickens. | flic, the flagellin antigen of salmonella enteritidis, was tested as a vaccine candidate for protective effect against a homologous challenge in chickens. after immunization with recombinant flic (rflic) or administration of phosphate-buffered saline (pbs) at 56 days old, the chickens were challenged with 10(9) colony-forming units of salmonella enteritidis at 76 days old. the vaccinated birds showed significantly decreased bacterial counts in the liver and cecal contents compared to those admin ... | 2012 | 22856193 |
| immune response to liposome-associated recombinant sef21 following oral immunization in chickens. | in order to generate salmonella enterica serovar enteritidis fimbriae antigens (rsef21), the intact region encoding sef21 was amplified from salmonella enteritidis by pcr and subcloned into a prokaryotic expression vector pet-28a(+) to yield pet-28a(+)-sef21. the rsef21 protein was highly expressed and purified by nickel affinity chromatography. liposomeassociated rsef21 was prepared for oral immunization to seek protective efficacy for intestinal infection with salmonella enteritidis. evidence ... | 2012 | 22856192 |
| effect of systemic infection induced by pseudomonas aeruginosa on the brain uptake of colistin in mice. | in view of reports of colistin-induced neurotoxicity in infected patients, the aim of this study was to assess whether the integrity of the blood-brain barrier (bbb) and the brain uptake of colistin are altered in the presence of systemic pseudomonas aeruginosa infection. bacteremia was confirmed 8 h after intramuscular administration of p. aeruginosa atcc 27853 to swiss outbred mice, at which time a single subcutaneous dose of colistin sulfate (40 mg/kg of body weight) or an intravenous dose of ... | 2012 | 22850514 |
| whole blood-derived microrna signatures in mice exposed to lipopolysaccharides. | lipopolysaccharide (lps) is recognized as the most potent microbial mediator presaging the threat of invasion of gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. this study was designed to examine the microrna (mirna) expression in whole blood from mice injected with intraperitoneal lps. | 2012 | 22849760 |
| influence of methodologic aspects on the results of implant-abutment interface microleakage tests: a critical review of in vitro studies. | this study sought to evaluate the influence of methodologic aspects on variations in the findings of in vitro microleakage studies of the implant-abutment interface. | 2015 | 22848880 |
| promoter strength driving tetr determines the regulatory properties of tet-controlled expression systems. | bacteria frequently rely on transcription repressors and activators to alter gene expression patterns in response to changes in the surrounding environment. tet repressor (tetr) is a paradigm transcription factor that senses the environmental state by binding small molecule effectors, the tetracyclines. however, recently isolated peptides that act as inducers of tetr after having been fused to the c-terminus of a carrier protein, suggest that tetr can also regulate gene expression in a signal-tr ... | 2012 | 22848546 |
| study on antibacterial alginate-stabilized copper nanoparticles by ft-ir and 2d-ir correlation spectroscopy. | the objective of this study was to clarify the intermolecular interaction between antibacterial copper nanoparticles (cu nps) and sodium alginate (naalg) by fourier transform infrared spectroscopy (ft-ir) and to process the spectra applying two-dimensional infrared (2d-ir) correlation analysis. to our knowledge, the addition of naalg as a stabilizer of copper nanoparticles has not been previously reported. it is expected that the obtained results will provide valuable additional information on: ... | 2012 | 22848180 |
| social networks and the spread of salmonella in a sleepy lizard population. | although theoretical models consider social networks as pathways for disease transmission, strong empirical support, particularly for indirectly transmitted parasites, is lacking for many wildlife populations. we found multiple genetic strains of the enteric bacterium salmonella enterica within a population of australian sleepy lizards (tiliqua rugosa), and we found that pairs of lizards that shared bacterial genotypes were more strongly connected in the social network than were pairs of lizards ... | 2012 | 22845647 |
| cpg oligonucleotides and recombinant interferon-γ in combination improve protection in chickens to salmonella enterica serovar enteritidis challenge as an adjuvant component, but have no effect in reducing salmonella carriage in infected chickens. | salmonella enterica serovar enteritidis is the most common cause of human salmonellosis in many developed nations. it is frequently associated with both poultry meat and eggs. in the present study we have determined whether cpg oligonucleotides that stimulate the immune system via toll like-receptors 15 and 21 in the chicken can be used as immunomodulatory agents to break carriage of s. enteritidis in in vitro and in vivo infection models. we also investigated its use as a component in an adjuva ... | 2012 | 22845324 |
| obacunone represses salmonella pathogenicity islands 1 and 2 in an envz-dependent fashion. | obacunone belongs to a class of unique triterpenoids called limonoids, present in citrus species. previous studies from our laboratory suggested that obacunone possesses antivirulence activity and demonstrates inhibition of cell-cell signaling in vibrio harveyi and escherichia coli o157:h7. the present work sought to determine the effect of obacunone on the food-borne pathogen salmonella enterica serovar typhimurium lt2 by using a cdna microarray. transcriptomic studies indicated that obacunone ... | 2012 | 22843534 |
| several enteropathogens are circulating in suckling and newly weaned piglets suffering from diarrhea in the province of villa clara, cuba. | intestinal contents of suckling (n = 45) and newly weaned (n = 45) piglets, suffering from diarrhea in the province of villa clara in cuba, were tested for viral, bacterial, and parasitic enteropathogens from may to june 2008. at least one enteropathogen was identified in 53.3 % of piglets and enterotoxigenic escherichia coli (etec; 25.6 %) was the major pathogen; mostly sta(+)/stb(+) or f4(+)/sta(+)/stb(+) etec were isolated. the overall occurrence of the rest of pathogens was 10 % for transmis ... | 2013 | 22843242 |
| combined genomarkers approach to salmonella characterization reveals that nucleotide sequence differences in the phase 1 flagellin gene flic are markers for variation within serotypes. | the characterization and tracking of pathogenic micro-organisms in the clinical laboratory and public health environment demand schemes that are easy to standardize and use, are automated and high-throughput, and provide portable data. a combined genomarkers approach for salmonella enterica based on comparative sequence analysis by mass spectrometry has been developed. the scheme targets genes encoding synthesis and assembly of antigens, metabolic pathway enzymes, virulence factors and fluoroqui ... | 2012 | 22837220 |
| on the different growth conditions affecting silver antimicrobial efficacy on listeria monocytogenes and salmonella enterica. | silver is known to inhibit microorganisms and therefore it is an ideal candidate for its incorporation in a wide variety of materials for food applications. however, there is still a need for understanding how silver prolonged exposure to bacterial contamination affects the bioavailability of the active silver species. in the present study, growth curves of listeria monocytogenes and salmonella enterica were performed for 3-5 days in tryptic soy broth (tsb) and m9 minimal medium (m9) in the pres ... | 2012 | 22835228 |
| salmonella prevalence among reptiles in a zoo education setting. | clinically healthy reptiles may shed salmonella and therefore act as a potential zoonotic threat. most people in northern european countries are rarely exposed to reptiles, but many zoos have education departments where children have direct contact with this group of animals. the objectives of this study were to determine the prevalence and serotype distribution of salmonella among reptiles in the education department (n = 55) at copenhagen zoo and compare it to the zoo's main reptile collection ... | 2013 | 22835051 |
| zinc prevents salmonella enterica serovar typhimurium-induced loss of intestinal mucosal barrier function in broiler chickens. | the study was carried out to evaluate the beneficial effects of supplemental zinc (zn) on the intestinal mucosal barrier function in salmonella enterica serovar typhimurium-challenged broiler chickens in a 42-day experiment. a total of 336 1-day-old male arbor acres broiler chicks were assigned to eight treatment groups. a 4×2 factorial arrangement of treatments was used in a completely randomized experimental design to study the effects of levels of supplemental zn (0, 40, 80 and 120 mg/kg diet ... | 2012 | 22834550 |
| epigenetic modification: possible approach to reduce salmonella enterica serovar enteritidis susceptibility under stress conditions. | stressors may influence chicken susceptibility to pathogens such as salmonella enterica. feed withdrawal stress can cause changes in normal intestinal epithelial structure and may lead to increased attachment and colonization of salmonella. this study aimed to investigate modulatory effects of epigenetic modification by feed restriction on s. enterica serovar enteritidis colonization in broiler chickens subjected to feed withdrawal stress. chicks were divided into four groups: ad libitum feeding ... | 2012 | 22834548 |
| characterization of five novel endolysins from gram-negative infecting bacteriophages. | we here characterize five globular endolysins, encoded by a set of gram-negative infecting bacteriophages: bcepc6gp22 (burkholderia cepacia phage bcepc6b), p2gp09 (escherichia coli phage p2), psp3gp10 (salmonella enterica phage psp3), k11gp3.5 and kp32gp15 (klebsiella pneumoniae phages k11 and kp32, respectively). in silico, bcepc6gp22, p2gp10 and psp3gp10 are predicted to possess lytic transglycosylase activity, whereas k11gp3.5 and kp32gp15 have putative amidase activity. all five endolysins s ... | 2013 | 22832988 |
| salmonella can reach tomato fruits on plants exposed to aerosols formed by rain. | outbreaks of salmonella enterica have been associated with tomatoes and traced back to production areas but the spread of salmonella in agricultural fields is still poorly understood. post-rain salmonella transfer from a point source to the air and then to tomato plants was evaluated. gfp-labeled kanamycin-resistant s. enterica serovar typhimurium (10(8)cfu/ml) with and without expression of the rdar morphotype (rough colonies; cells with fimbriae and cellulose) was used as the point source in t ... | 2012 | 22831820 |
| the nsrr regulon in nitrosative stress resistance of salmonella enterica serovar typhimurium. | nitric oxide (no·) is an important mediator of innate immunity. the facultative intracellular pathogen salmonella has evolved mechanisms to detoxify and evade the antimicrobial actions of host-derived no· produced during infection. expression of the no·-detoxifying flavohaemoglobin hmp is controlled by the no·-sensing transcriptional repressor nsrr and is required for salmonella virulence. in this study we show that nsrr responds to very low no· concentrations, suggesting that it plays a primary ... | 2012 | 22831173 |
| supported lipopolysaccharide bilayers. | in this report, the formation of supported lipopolysaccharide bilayers (lps-slbs) is studied with extracted native and glycoengineered lps from escherichia coli ( e. coli ) and salmonella enterica sv typhimurium ( s. typhimurium ) to assemble a platform that allows measurement of lps membrane structure and the detection of membrane tethered saccharide-protein interactions. we present quartz crystal microbalance with dissipation monitoring (qcm-d) and fluorescence recovery after photobleaching (f ... | 2012 | 22830310 |
| reduction in resident microflora, and experimentally inoculated salmonella enterica, on spinach leaves treated with vinegar and canola oil. | in this study, we explored the use of vinegar, or vinegar and canola oil as a salad dressing, to reduce bacterial levels on spinach leaves. we found that incubation of spinach leaves with various types of vinegar substantially reduced the predominantly gram-negative microflora. a similar response was observed when spinach leaves were incubated with white vinegar mixed in various proportions with canola oil, as used in salad dressing. we assessed the effects of vinegar, or vinegar and oil, on spi ... | 2012 | 22828283 |