Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| rehospitalization with respiratory syncytial virus after neonatal intensive care unit discharge: a 3-year follow-up. | this study sought to determine 1) the incidence of rehospitalization with respiratory syncytial virus (rsv) infection within a 3-year follow-up among infants discharged from the neonatal intensive care unit (nicu), and 2) to examine associations between age at readmission and medical and sociodemographic characteristics among infants rehospitalized with rsv. | 1997 | 9382909 |
| crystals of rous sarcoma virus capsid protein show a helical arrangement of protein subunits. | crystals of rous sarcoma virus (rsv) capsid protein diffract x rays to 3.5 a resolution and belong to the monoclinic space group c2 with unit cell parameters a = 374.4 a, b = 128.1 a, c = 200.2 a, and beta = 121.8 degrees. one asymmetric unit of the crystal may contain between 28 and 35 molecules, based on reasonable crystal density assumptions. a self-rotation function and patterson synthesis suggest that rsv capsid protein crystallizes as a helical array. the determinants of the viral particle ... | 1997 | 9375011 |
| nosocomial respiratory syncytial virus infection in canadian pediatric hospitals: a pediatric investigators collaborative network on infections in canada study. | to determine nosocomial transmission of respiratory syncytial virus (rsv) in canadian pediatric hospitals, outcomes associated with nosocomial disease, and infection control practices. | 1997 | 9374561 |
| respiratory syncytial virus immune globulin treatment of rsv lower respiratory tract infection in previously healthy children. | to evaluate the efficacy of high titer respiratory syncytial virus (rsv) immune globulin (rsvig) in the treatment of previously healthy children hospitalized with proven rsv lower tract infection (lri). | 1997 | 9374560 |
| human monoclonal fab fragments specific for viral antigens from combinatorial iga libraries. | iga constitutes the first line of immune defense, interacting with a variety of environmental antigens. following infection with respiratory syncytial virus (rsv) individuals frequently exhibit elevated serum iga titers specific for the virus. previously combinatorial igg libraries have successfully been used to clone such human antibody responses. | 1995 | 9373330 |
| cloning, expression and functional activities of a single chain antibody fragment directed to fusion protein of respiratory syncytial virus. | hnk20 is a murine iga which is currently being investigated in clinical trials against respiratory syncytial virus (rsv) infections in infants and young children. | 1996 | 9373314 |
| rous sarcoma virus direct repeat cis elements exert effects at several points in the virus life cycle. | two approximately 135-nucleotide (nt) direct repeats flank the rous sarcoma virus (rsv) oncogene src and are composed of two smaller repeats, dr1 (approximately 100 nt) and dr2 (approximately 36 nt). these sequences have been reported to contain cis-acting signals necessary for rna packaging and elements that allow cytoplasmic accumulation of unspliced rna (cytoplasmic transport elements). in this report, we show that avian fibroblasts infected with the prague a strain of rsv with precise deleti ... | 1997 | 9371572 |
| recombinant respiratory syncytial virus from which the entire sh gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse. | the small hydrophobic protein sh of human respiratory syncytial virus (rsv) is a short transmembrane surface protein of unknown function. a full-length cdna of rsv strain a2 (subgroup a) antigenomic rna was modified such that the entire sh gene, including the transcription signals and the complete mrna-encoding sequence, was deleted and replaced by a synthetic intergenic region. this reduced the length of the antigenome by 398 nucleotides and ablated expression of 1 of the 10 rsv mrnas. recombin ... | 1997 | 9371553 |
| efficacy of bronchodilators in the treatment of bronchiolitis. | bronchiolitis is a common respiratory infection affecting young children. much controversy revolves around the efficacy of bronchodilators in the treatment of bronchiolitis. this study was conducted to address this issue. | 1997 | 9364884 |
| influence of maternal infections with viral agents or toxoplasma gondii during pregnancy on fetal ige production. | the importance of maternal infections with toxoplasma gondii, cytomegalovirus (cmv), parvovirus b19, respiratory syncytial virus (rsv), and influenza a and b on fetal ige synthesis was studied in 153 pregnant women. no case of specific igm activity or viral dna in cord blood, indicating a congenital infection, was found. from gestational week 15 to delivery, maternal igg-ab seroconversion to parvovirus b19, rsv, influenza a, or influenza b occurred in 47 women. at delivery, serologic signs of pa ... | 1997 | 9360748 |
| development of a humanized monoclonal antibody (medi-493) with potent in vitro and in vivo activity against respiratory syncytial virus. | neutralizing polyclonal antibody to respiratory syncytial virus (rsv) has been shown to be an effective prophylactic agent when administered intravenously in high-risk infants. this study describes the generation of a humanized monoclonal antibody, medi-493, that recognizes a conserved neutralizing epitope on the f glycoprotein of rsv. the affinity of medi-493 was found to be equal to or slightly better than an isotype-matched chimeric derivative of the parent antibody. in plaque reduction, micr ... | 1997 | 9359721 |
| systemic and local immune responses of four cases with lower respiratory tract illness due to reinfection with respiratory syncytial virus. | four infants and children who had tracheobronchitis or bronchiolitis by respiratory syncytial virus (rsv) reinfection were presented. their clinical features, systemic and local immune reactions to rsv were compared with those of eleven age-matched patients who had lower respiratory tract illness with primary rsv infection. moderate antibody activities in their nasopharyngeal secretions and sera which were comparable to those of convalescent phase of primary infection were observed within severa ... | 1997 | 9354358 |
| simplified, rapid diagnosis of respiratory syncytial virus from clinical specimens. | dot elisa was compared with rt-pcr and tissue culture to detect rsv from nasopharyngeal aspirates. dot elisa had diagnostic sensitivity and specificity of 65.62% and 93.92%, respectively. the results indicate that dot elisa can be used for screening detection of rsv from clinical specimens and is suitable for small laboratories in the provincial areas of developing countries. | 1997 | 9346274 |
| overexpression of interleukin-4 delays virus clearance in mice infected with respiratory syncytial virus. | although interleukin-4 (il-4) expression has been implicated in vaccine-enhanced respiratory syncytial virus (rsv) disease, its role in mediating the immune response to primary rsv infection remains unclear. to assess the effect of il-4 production on typical rsv infection, transgenic mice which either overexpress or fail to express il-4 were challenged intranasally with rsv and their responses were compared to those of the parent strains. il-4-deficient mice eliminated virus from the lung as qui ... | 1997 | 9343225 |
| current status of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccine development: memorandum from a joint who/niaid meeting. | a joint who/national institute of allergy and infectious diseases (niaid) meeting on the current status of respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3) vaccine development was held in bethesda, md, from 30 september to 1 october 1996. the meeting summarized the worldwide impact of rsv and piv3; presented the current status of development of rsv and piv3 vaccines; and examined the applications of recombinant dna technology to the development and characterization of vacc ... | 1997 | 9342889 |
| comparison of various expression plasmids for the induction of immune response by dna immunization. | intramuscular injection of plasmid dna is an efficient method to introduce a foreign gene into a live animal. we investigated several factors affecting the gene transfer efficiency and the following immune response by intramuscular injection of plasmid dna. when the strength of several highly efficient viral promoters was compared in muscle by using the chloramphenicol acetyltransferase (cat) gene as an indicator, cytomegalovirus (cmv) immediate early promoter was found to be stronger than any o ... | 1997 | 9339893 |
| allergic sensitization increases airway reactivity in guinea pigs with respiratory syncytial virus bronchiolitis. | respiratory syncytial virus (rsv) causes acute bronchiolitis in children and has been implicated in the pathogenesis of recurrent wheezing and asthma. however, few children exposed to rsv experience acute bronchiolitis or its sequelae, suggesting a subgroup of susceptible children. an allergic diathesis may predispose children to subsequent airway disease. | 1997 | 9338543 |
| uptake of plasmid rsv dna by frog and mouse spermatozoa. | xenopus laevis spermatozoa and mouse epididymal spermatozoa were compared in their ability to bind plasmid dna. spermatozoa of both species are endowed with a very similar binding capacity for plasmid paprc dna carrying the complete rous sarcoma virus (rsv) dna. our experiments failed to detect any substantial differences between both types of sperm cells in the kinetics of dna binding, maximum dna binding capacity, effect of various ions, metabolic inhibitors and substrates on dna binding, etc. ... | 1997 | 9338119 |
| constitutive expression of murine ctla4ig from a recombinant adenovirus vector results in prolonged transgene expression. | the administration of soluble muctla4ig around the time of adenovirus vector mediated gene transfer into murine hepatocytes has been shown to markedly prolong transgene expression, diminish the formation of adenovirus neutralizing antibody, decrease t cell proliferative response and infiltration into the liver without causing irreversible systemic immunosuppression. in this study, an e1/e3-deleted adenovirus vector constitutively expressing murine ctla4ig (ad.rsv-muctla4ig) was constructed in or ... | 1997 | 9338015 |
| adenovirus-mediated gene transfer of a human il-6 antagonist. | il-6 is a pleiotropic cytokine and plays a major role in inflammation and in the immune response. altered serum levels of il-6 have been described in several pathologies such as myeloma, ebv-lymphoma and chronic autoimmune disease. here we report data on the utilization of a hil-6 receptor superantagonist with a gene therapy approach. the superantagonist used in this work possesses very high affinity for the hil-6 receptor, and is therefore an excellent candidate for the treatment of il-6-depend ... | 1997 | 9338013 |
| home-based immunoglobulin therapy reduces incidence of neonatal rsv infection. | respiratory syncytial virus (rsv) infections are responsible for more than 90,000 hospitalizations and 4500 infant and childhood deaths each year. rsv infection, which peaks between january and march, can cause respiratory problems, such as bronchiolitis and pneumonia, in children younger than 2 years old. | 1997 | 9335686 |
| contribution of tumor necrosis factor alpha and interleukin-1 alpha on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in a murine macrophage cell line, raw264.7. | the production of several inflammatory cytokines, such as murine macrophage inflammatory protein-2 (mip-2), tumor necrosis factor (tnf), and interleukin (il)-1, was investigated in response to respiratory syncytial virus (rsv) infection in a murine macrophage cell line, raw264.7, with special reference to mutual relation of their productions. the kinetics of mip-2 production showed a trend for a biphasic pattern, that is, mip-2 levels became detectable from 2 h postinfection (p.i.) and increased ... | 1997 | 9334925 |
| protective immunity against respiratory syncytial virus in early life after murine maternal or neonatal vaccination with the recombinant g fusion protein bbg2na. | maternal and neonatal immunization were evaluated for their capacity to induce protective immunity against respiratory syncytial virus (rsv) lower respiratory tract infections in early life. murine models were studied by use of a novel recombinant vaccine candidate, designated bbg2na, which was derived in part from the rsv (long) g protein. maternal immunization resulted in the passive transfer of high levels of rsv-a antibodies to the offspring, which protected them from rsv challenge for up to ... | 1997 | 9333145 |
| analysis of respiratory syncytial virus in clinical samples by reverse transcriptase-polymerase chain reaction restriction mapping. | the aim of this study was to develop a polymerase chain reaction (pcr) for the detection of respiratory syncytial virus (rsv) genomes. the primers were designed from published sequences and selected from conserved regions of the genome encoding for the n protein of subgroups a and b of rsv. pcr was applied to 20 specimens from children admitted to the respiratory ward of "william soler" pediatric hospital in havana city with a clinical diagnosis of bronchiolitis. the pcr was compared with viral ... | 1997 | 9332606 |
| rod-shaped virus-like particles in intestinal contents of pheasant chicks. | rod-shaped virus-like particles (rsv), 55-85 nm in length and 18 nm in diameter, with 5 to 10 segments or helical turns, were demonstrated in the intestinal contents of young diarrhoeic pheasants by examination of a fresh sample. the origin of rsv seems to be splitting tails of bacteriophages. | 1997 | 9323932 |
| the incidence of respiratory tract infection in adults requiring hospitalization for asthma. | acute respiratory tract infections (rti) are known to worsen asthma particularly in children. there are few studies in adults assessing the incidence of rti in patients hospitalized with acute asthma. | 1997 | 9315789 |
| [viruses precipitating asthmatic crises]. | study made with 45 patients in the allergy service of the instituto nacional de pediatría, with the purpose of analyzing the virus participation in the precipitation of asthmatic crisis in children who lived in the southeast of mexico city. the subjects were included in the study if they presented signs of atopy or infection of the superior air channels. pollen allergics, rhinosinusitis, smokers relatives and patients who own pets were excluded. in this population group, the participation of the ... | 1993 | 9312342 |
| selection of an avian retrovirus mutant with extended receptor usage. | receptor recognition by avian retroviruses is thought to involve the interaction of two regions of the su protein, hr1 and hr2, with the host cell surface receptor. these regions exhibit considerable variation, concordant with differences in receptor usage among the many avian leukosis virus subgroups. we hypothesize that some retroviruses have altered receptor usage in response to selective pressures imposed by receptor polymorphisms in their hosts. to test this hypothesis, we passaged td-pr-rs ... | 1997 | 9311868 |
| multiple biological roles associated with the rous sarcoma virus 5' untranslated rna u5-ir stem and loop. | the 5' untranslated region of rous sarcoma virus (rsv) rna is a highly ordered structure involved in multiple processes in the viral replication cycle. one of these structures, referred to as the u5-ir stem, is located immediately upstream of the 5' end of the primer binding site. disruption of its base pairing results in a decrease in initiation of reverse transcription (d. cobrinik, a. aiyar, z. ge, m. katzman, h. huang, and j. leis, j. virol. 65:3864-3872, 1991). in the present study, the len ... | 1997 | 9311847 |
| usefulness of a mouse myelin basic protein promoter for gene therapy of malignant glioma: myelin basic protein promoter is strongly active in human malignant glioma cells. | we have searched for suitable promoters to regulate the expression of suicide genes for use in gene therapy. we have shown that the 1.3-kb fragment of the mouse myelin basic protein (mbp) promoter region initiates transcription in mouse glioma cells more efficiently than glial fibrillary acidic protein (gfap) or myelin proteolipid protein (plp) promoter. among three different lengths of the mbp promoter, the shortest (256-bp) core promoter region initiates transcription as efficiently as 650-bp ... | 1997 | 9310141 |
| effect of rapid viral diagnosis on the management of children hospitalized with lower respiratory tract infection. | although rapid viral tests are commonly used in children with lower respiratory tract infection, their effect on patient management has not been studied. | 1997 | 9306477 |
| immunization with a peptide derived from the g glycoprotein of bovine respiratory syncytial virus (brsv) reduces the incidence of brsv-associated pneumonia in the natural host. | previous reports demonstrate that synthetic peptides corresponding to the amino acid region 174-187 of g glycoprotein from subgroups a and b human respiratory syncytial virus (hrsv), containing a cys-->ser substitution at position 186, confer complete resistance to immunized balb/c mice against infection with the respective virus. in this report, we show that a cys186-->ser substituted peptide (bg/174-187) representing the corresponding region of the bovine (b) rsv g glycoprotein conferred compl ... | 1997 | 9302749 |
| molecular characterization of an outbreak of respiratory syncytial virus (subgroup a) in havana, cuba, by monoclonal antibodies and restriction mapping (n gene). | twenty-one respiratory syncytial virus (rsv) strains isolated from one outbreak in havana, cuba (1994 to 1995), were analyzed to determine their relatedness. all isolated strains were classified as subgroup a by monoclonal antibodies. of 21 rsv strains examined, 20 were classified as having restriction pattern np4 and only 1 was classified as having restriction pattern np5. | 1997 | 9302221 |
| increased expression of the n protein of respiratory syncytial virus stimulates minigenome replication but does not alter the balance between the synthesis of mrna and antigenome. | a popular model for rna synthesis by nonsegmented negative-strand rna viruses is that transcription and rna replication are executed by the same polymerase complex and that there is a dynamic balance between the two processes that is mediated by the nucleocapsid n protein. according to this model, transcription occurs until sufficient soluble n protein accumulates to initiate encapsidation of the nascent rna product, which somehow switches the polymerase into a readthrough replicative mode. this ... | 1997 | 9299631 |
| challenge of balb/c mice with respiratory syncytial virus does not enhance the th2 pathway induced after immunization with a recombinant g fusion protein, bbg2na, in aluminum hydroxide. | the polypeptide of aa 130-230 of the g protein (g2na) of respiratory syncytial virus (rsv) was fused to bb, the albumin-binding region of streptococcal g protein, producing bbg2na, which induced protective immune responses in rodent models. evaluation of the immune response in mice immunized with bbg2na in the adjuvant alhydrogel revealed high amounts of interleukin (il)-5 and some il-4 in splenocytes restimulated in vitro. this is compatible with a th2 response. the activation of the th2 pathwa ... | 1997 | 9291300 |
| ex vivo adenovirus-mediated gene transfer and immunomodulatory protein production in human cornea. | one attractive strategy to prevent or control allograft rejection is to genetically modify the donor tissue before transplantation. in this study, we have examined the feasibility of gene transfer to human corneal endothelium, using a number of recombinant adenovirus constructs. ex vivo infection of human corneas with adenoviral vectors containing lacz, under transcriptional control of either cytomegalovirus (cmv) or rous sarcoma virus (rsv) promoters, provided high-level gene expression, which ... | 1997 | 9282165 |
| interrelationship between tumour virus receptors, a and c, coded by host cell genes in fowl. | bryan standard strain of rous sarcoma virus (bs-rsv) of subgroup a and pseudotype of bryan high titre rsv(rav-49) of subgroup c and an equal mixture of subgroup a and subgroup c virus were inoculated to 11-day old embryos of white leghorn (wl), australorp (al) and f1 and f2 generations of crosses between wl and al breeds of fowl to detect and estimate the interrelationship between tumour virus receptor coding host cell genes of tva and tvc loci. linkage values estimated on a pooled sex basis wer ... | 1997 | 9279130 |
| a monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus antigens in formalin-fixed, paraffin-embedded tissue. | four monoclonal antibodies (mabs) with specificities for epitopes on human respiratory syncytial virus (rsv) proteins preserved after formalin fixation and paraffin embedding were identified in fixed and embedded virus-infected hep-2 cell pellets. the mabs bound epitopes on the fusion protein, the nucleoprotein, the phosphoprotein, and the m2 protein of the virus. following high-temperature antigen unmasking, immunohistochemical staining revealed rsv antigens in the lungs of five of seven childr ... | 1997 | 9274537 |
| inflammatory cells and activation markers in bal during acute rejection and infection in lung transplant recipients: a prospective, longitudinal study. | acute rejection of the transplanted lung is a clinical problem, since it decreases graft survival and predisposes the patient to chronic rejection and obliterative bronchiolitis (ob). in an earlier study, we had indications that eosinophil cationic protein (ecp) from activated eosinophils and hyaluronan (hya) from fibroblasts were associated with acute pulmonary rejection. this prospective longitudinal study was designed to investigate whether molecules from activated inflammatory cells in bronc ... | 1997 | 9272913 |
| prevalence of respiratory viruses and mycoplasma pneumoniae in sputum samples from unselected adult patients. | sputum samples from adult patients are routinely used for bacteriological tests, but not for the diagnosis of viral/mycoplasmal infections. we examined 511 sputum samples submitted for bacterial tests from patients at the royal adelaide hospital. each specimen was tested directly (and after six days of cell culture amplification) for antigens to influenza a and b, parainfluenza 1, 2 and 3, adenovirus, respiratory syncytial virus (rsv) and mycoplasma pneumoniae. respiratory viruses or m. pneumoni ... | 1997 | 9271022 |
| safety and immunogenicity of a respiratory syncytial virus subunit vaccine (pfp-2) in the institutionalized elderly. | the safety and immunogenicity of purified fusion protein (pfp-2) respiratory syncytial virus (rsv) vaccine was evaluated in an open label study in 37 frail institutionalized persons over age 65. vaccination was well tolerated without significant side-effects. thirty-six of 37 volunteers completed the study. nineteen of 36 (53%) vaccinees had a greater than or equal to fourfold increase in igg to f protein at 4 weeks and 17 (47%) had a greater than or equal to fourfold rise in neutralizing titers ... | 1997 | 9269057 |
| analysis of bovine respiratory syncytial virus envelope glycoproteins in cell fusion. | to compare the requirements for respiratory syncytial virus (rsv)-mediated cell fusion, the fusion (f), attachment (g) and small hydrophobic (sh) glycoproteins of bovine rsv (brsv), ovine rsv (orsv) and human rsv (hrsv) were expressed individually or coexpressed in either homologous or heterologous combinations in hela cells, using the vaccinia virus-t7 polymerase transient expression system. cell fusion was examined by a reporter gene activation assay. although the expression of the f protein a ... | 1997 | 9266983 |
| immortalized cell lines from embryonic avian and murine otocysts: tools for molecular studies of the developing inner ear. | recently, our studies have focused on genes expressed at the earliest stages of inner ear development. our aim is to identify and characterize genes that are involved in determining the axes of the semicircular canals, in otic crest delamination and in early innervation of the inner ear. many elegant studies of auditory development have been done in animal models. however, the need for large amounts of well-characterized embryonic material for molecular studies makes the development of otocyst c ... | 1997 | 9263030 |
| surfactant dysfunction develops in balb/c mice infected with respiratory syncytial virus. | recent reports suggest an important role for pulmonary surfactant in maintaining the patency of narrow conducting airways. the hypothesis that surfactant dysfunction is an important factor in respiratory syncytial virus (rsv) infection was tested in a mouse model. mice, inoculated with either a low or a high dose of rsv, were subjected to bronchoalveolar lavage (bal), and the fluids were analyzed for percentage of inflammatory cells and concentrations of proteins and phospholipids. after concent ... | 1997 | 9262218 |
| priming with live respiratory syncytial virus (rsv) prevents the enhanced pulmonary inflammatory response seen after rsv challenge in balb/c mice immunized with formalin-inactivated rsv. | to investigate enhanced disease associated with a formalin-inactivated (fi) respiratory syncytial virus (rsv) vaccine, we studied the pulmonary inflammatory response to rsv in balb/c mice immunized with live rsv, fi-rsv, or combinations of the two. after rsv challenge, the number of granular cells, the ratio of cd4+/cd8+ lymphocytes, and the level of th2-like cytokine mrnas in the bronchoalveolar lavage specimens in mice immunized first with live rsv and then with fi-rsv were lower than that in ... | 1997 | 9261421 |
| equine infectious anemia virus utilizes a yxxl motif within the late assembly domain of the gag p9 protein. | we have previously demonstrated that the gag p9 protein of equine infectious anemia virus (eiav) is functionally homologous with rous sarcoma virus (rsv) p2b and human immunodeficiency virus type 1 (hiv-1) p6 in providing a critical late assembly function in rsv gag-mediated budding from transfected cos-1 cells (l. j. parent et al., j. virol. 69:5455-5460, 1995). in light of the absence of amino acid sequence homology between eiav p9 and the functional homologs of rsv and hiv-1, we have now desi ... | 1997 | 9261374 |
| association between pronounced iga response in rsv bronchiolitis and development of allergic sensitization. | forty-five children who had been hospitalized with bronchiolitis caused by respiratory syncytial virus (rsv) at a mean age of 4 months, and 90 matched control children, were tested for occurrence of rsv antibodies at one year of age. of the children who had suffered from bronchiolitis, forty had demonstrable igg antibodies, whereas the remaining five only had iga antibodies against rsv. in the control group, 42% were rsv seropositive. the anti-rsv iga antibody titres tended to be higher in patie ... | 1997 | 9260211 |
| economic evaluation of respiratory syncytial virus infection in canadian children: a pediatric investigators collaborative network on infections in canada (picnic) study. | to quantify the cost and distribution of health care resources consumed annually in management of canadian children from birth to 4 years of age with respiratory syncytial virus (rsv) infection. | 1997 | 9255201 |
| targeted transduction of cns neurons with adenoviral vectors carrying neurotrophic factor genes confers neuroprotection that exceeds the transduced population. | application of neurotrophic factors (nfs) to the cut stump of motor nerves of neonatal rats confers neuroprotection from trauma-induced neuronal death. to test whether motoneurons are capable of responding to endogenously produced nfs, facial motoneurons were genetically modified in vivo to express several nfs and then tested for their response to peripheral nerve damage. replication-defective adenoviral vectors [adv. rous sarcoma virus (rsv)-nf] representing three families of nfs were construct ... | 1997 | 9254662 |
| monoclonal antibodies against respiratory syncytial virus for rapid detection and subgroup determination. | thirty-one hybridomas producing monoclonal antibodies (mabs) against structural proteins of rsv subgroup a (long strain) and rsv subgroup b (japanese wild strain) were produced and separated into three groups by their reactivities with rsv-a and rsv-b using ifa. group i was specific to rsv-a, group ii was specific to rsv-b and group iii was specific to both subgroups. characterization of selected two mabs from each group indicated that three mabs recognized phosphoprotein (p) and the others reco ... | 1996 | 9253866 |
| the effect of promoter strength in adenoviral vectors containing herpes simplex virus thymidine kinase on cancer gene therapy in vitro and in vivo. | the use of adenoviral vectors to deliver the herpes simplex virus thymidine kinase (hsvtk) gene followed by treatment with the prodrug ganciclovir (gcv) has promise for a variety of applications where excess cell proliferation is detrimental such as treatment of tumors and vascular restenosis. optimizing this system is thus an important goal. the purpose of this study was to determine if the induction of higher levels of hsvtk expression would augment the sensitivity to gcv. this was accomplishe ... | 1997 | 9253506 |
| detection of respiratory syncytial virus, parainfluenzavirus 3, adenovirus and rhinovirus sequences in respiratory tract of infants by polymerase chain reaction and hybridization. | immunofluorescence assay (ifa) of viral antigens in nasal aspirates is largely used for the diagnosis of respiratory syncytial virus (rsv), parainfluenzavirus (piv) type 3 and adenovirus (adv) infections, whilst rhinovirus (rv) are detected by virus isolation technique (vit) only. using the two techniques, ifa and vit, a significant number of specimens remain negative in spite of clinical and epidemiological presumptions of viral infection. | 1997 | 9248656 |
| macrophages and respiratory viruses. | this review highlights some of the interactions between alveolar macrophages and human/swine influenza a viruses, porcine reproductive and respiratory syndrome virus (prrsv), bovine parainfluenza type-3 (pi-3) virus, bovine respiratory syncytial virus (brsv) and bovine viral diarrhoea virus (bvdv). in influenza a infection, macrophage-cytokines are believed to mediate the typical constitutional effects and inflammation in the respiratory tract. the same cytokines possibly play a role in influenz ... | 1997 | 9247041 |
| randomised double blind placebo controlled trial of prednisolone in children admitted to hospital with respiratory syncytial virus bronchiolitis. | experimental and clinical evidence suggests that respiratory syncytial virus (rsv) bronchiolitis is an immune mediated disease. corticosteroids might therefore be effective in the treatment of rsv bronchiolitis. | 1997 | 9246136 |
| virus-specific cd8+ t lymphocytes downregulate t helper cell type 2 cytokine secretion and pulmonary eosinophilia during experimental murine respiratory syncytial virus infection. | t lymphocytes play a pivotal role in the immune response during viral infections. in a murine model of experimental respiratory syncytial virus (rsv) infection, mice sensitized to either of the two major glycoproteins of rsv develop distinct patterns of cytokine secretion and lung inflammation upon subsequent rsv infection. mice sensitized to rsv-g (attachment) glycoprotein exhibit a strong interleukin (il)-4 and il-5 response and develop pulmonary eosinophilia, whereas mice sensitized to rsv-f ... | 1997 | 9236194 |
| protective immune responses induced by the immunization of mice with a recombinant bacteriophage displaying an epitope of the human respiratory syncytial virus. | we investigated whether a recombinant bacteriophage displaying a disease-specific protective epitope could be experimentally used as a vaccine to confer protection of immunized animals against infection. we genetically engineered a recombinant phage, fd, displaying at its surface a chimeric piii coat protein fused to the previously identified protective epitope 173-187 from the glycoprotein g of the human respiratory syncytial virus (rsv). a selected recombinant fd phage elicited a strong immune ... | 1997 | 9234952 |
| transcriptional and post-transcriptional regulation of kappab-controlled genes by pp60v-src. | the cef-4/9e3 gene is expressed aberrantly in chicken embryo fibroblasts transformed by the rous sarcoma virus. this aberrant expression is dependent on transcriptional activation and on the stabilization of the cef-4 mrna. the characterization of the cef-4 promoter indicated that three distinct regulatory elements corresponding to an ap-1 binding site, a prdii/ kappab domain and a caat box are involved in the activation by pp60v-src. several v-src responsive genes are controlled by ap-1 and mem ... | 1997 | 9233775 |
| peptide mimics of a conformationally constrained protective epitopes of respiratory syncytial virus fusion protein. | to identify peptides that mimic (mimotopesi conformational and protective epitopes of rsv fusion protein and to assess their efficacy as immunogens and potential vaccines. | 1997 | 9232419 |
| respiratory syncytial virus infection in infants is associated with predominant th-2-like response. | viral infections have been associated with cellular immune responses and production of th-1 cytokines. respiratory syncytial virus (rsv), however, induces virus-specific ige, which might be a consequence of a th-2-like activation. to test this hypothesis we quantified interferon-gamma (ifn-gamma) and interleukin-4 (il-4) in the supernatant of peripheral blood mononuclear cells cultured for 24 and 48 h in the presence or absence of phytohemaglutinin and pokeweed mitogen and the lymphocyte phenoty ... | 1997 | 9230746 |
| heparin-dependent attachment of respiratory syncytial virus (rsv) to host cells. | in this study we could demonstrate that heparin (ed50 = 0.32 +/- 0.12 microgram/ml), but not heparan sulphate or chondroitin sulphate c is able to inhibit in vitro infection of cells by respiratory syncytial virus (rsv). in addition, this protective effect of heparin could only be observed, when heparin was present at the time of inoculation. enzymatic digestion of cell surface glycosaminoglycans with heparinase and heparitinase, but not chondroitin sulphate abc lyase reduced the effectiveness o ... | 1997 | 9229012 |
| respiratory syncytial virus: not just for kids. | respiratory syncytial virus (rsv) earned its reputation as a pediatric pathogen, especially in children under two years of age. rsv infection in adults was considered a significant problem only for certain high-risk populations. evidence now indicates that the infection occurs frequently in previously healthy adults. clinical presentation varies greatly. | 1997 | 9227660 |
| the temperature-sensitive (ts) phenotype of a cold-passaged (cp) live attenuated respiratory syncytial virus vaccine candidate, designated cpts530, results from a single amino acid substitution in the l protein. | cpts530, a candidate live-virus vaccine, is an attenuated strain of human respiratory syncytial virus (rsv). it was derived by subjecting a cold-passaged (cp) strain of rsv to a single round of chemical mutagenesis. cpts530 is a temperature-sensitive (ts) mutant that is attenuated in mice and chimpanzees, and its ts phenotype exhibits a high level of stability during replication in both species. in the present study, the complete nucleotide sequence of cpts530 rsv was determined. the five mutati ... | 1997 | 9223470 |
| replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo. | replication-competent retroviral vectors based on rous sarcoma virus (rsv) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. in this article, we review the features of rsv and its life cycle that make it suitable for use as a vector. we describe the design and use of the rcas and rcas (bp) series of vectors, which are currently the most widely used rsv-based vectors, illustrating both their strengths and weakness. finally, we ... | 1997 | 9219242 |
| adenovirus and respiratory syncytial virus in chronic sinusitis using polymerase chain reaction. | the aim of this study is to investigate the role of adenovirus and respiratory syncytial virus (rsv) in chronic sinusitis using the polymerase chain reaction (pcr) to assay for the viruses. pcr has proved to be more sensitive and specific than viral cultures and immunoassays in the detection of viruses. adenovirus and rsv are among the most common viruses to cause upper respiratory tract infections. sinus mucosa biopsies from 20 patients undergoing endoscopic sinus surgery were sterilely collect ... | 1997 | 9217132 |
| adenoviral-mediated herpes simplex virus thymidine kinase gene transfer: regression of hepatic metastasis of pancreatic tumors. | pancreatic cancer is the fifth leading cause of cancer death in the united states. most patients have obvious metastases or locally advanced disease at the time of presentation. surgical resection does not significantly change the clinical outcome. combination chemotherapy induces a partial response but overall survival remains low. the aim of this study was to evaluate the feasibility of adenovirus-mediated suicide gene transduction as a therapeutic approach for pancreatic cancer. a cell line w ... | 1997 | 9211489 |
| baculovirus expression of the respiratory syncytial virus fusion protein using trichoplusia ni insect cells. | respiratory syncytial virus (rsv) is a major viral pathogen responsible for severe respiratory tract infections in infants, young children, and the elderly. the rsv fusion (f) protein is highly conserved among rsv subgroups a and b and is the major protective immunogen. a genetically-engineered version of the rsv f protein was produced in insect cells using the baculovirus expression system. to express a secreted form of this protein, the transmembrane domain was eliminated by removing the regio ... | 1997 | 9208456 |
| prevalence of antibodies against respiratory viruses (parainfluenza virus type 3, respiratory syncytial virus, reovirus and adenovirus) in relation to productivity in syrian awassi sheep. | awassi sheep sera from all the provinces of the syrian arab republic were tested for rsv, p13, reo and adeno viruses (iif and agid tests). rsv was the most prevalent infection with 63.6% of samples seropositive, followed by reo, p13 and adenovirus with seroprevalences of 27.3%, 24% and 8.1% respectively. animals were more frequently infected by rsv alone. mixed infections were also identified but the occurrence was not high. the rsv and reo virus infections occurred more frequently when transhum ... | 1997 | 9203308 |
| construction of recombinant adeno-associated virus vector containing the rat preproinsulin ii gene. | we have investigated a possible delivery system for the rat preproinsulin ii gene (ri2) utilising a recombinant adeno-associated virus (raav) vector system, with the long-term goal of engineering stably infected insulin-producing cell lines. the raav vector was chosen because it is a safe and nonpathogenic method for gene transfer. the plasmid pbc12bi (atcc) was purified and digested with restriction enzymes sepi and stui to release a fragment containing the rous sarcoma virus long terminal repe ... | 1997 | 9202669 |
| regulated expression of the diphtheria toxin a gene in human glioma cells using prokaryotic transcriptional control elements. | because accurate regulation of toxin gene expression is critical for safe and effective gene therapy applications, the authors have examined the regulation of diphtheria toxin a (dta) fragment expression in human glioma cell lines using two transcriptional control systems derived from escherichia coli: the tetracycline (tet) system and the lactose (lac) system. the tet system includes a tetracycline-controlled transactivator (tta), a tta-responsive minimum human cytomegalovirus (hcmv) promoter c ... | 1997 | 9202271 |
| respiratory syncytial virus infection results in airway hyperresponsiveness and enhanced airway sensitization to allergen. | viral respiratory infections can predispose to the development of asthma by mechanisms that are presently undetermined. using a murine model of respiratory syncytial virus (rsv) infection, acute infection is associated with airway hyperresponsiveness as well as enhanced responses to subsequent sensitization to allergen. we demonstrate that acute viral infection results in increased airway responsiveness to inhaled methacholine and pulmonary neutrophilic and eosinophilic inflammation. this respon ... | 1997 | 9202075 |
| il-12 treatment attenuates t helper cell type 2 and b cell responses but does not improve vaccine-enhanced lung illness. | in humans and mice, sensitization to respiratory syncytial virus (rsv) ags can result in severe inflammatory lung disease during subsequent infection with rsv. although specific antiviral t cells are thought to be responsible for this augmentation of disease, the precise roles of different functional subsets are unknown, and no protective nonpathogenic subset has been defined. balb/c mice sensitized to the major surface glycoprotein of rsv (g) expressed by recombinant vaccinia virus develop th2- ... | 1997 | 9200470 |
| determination of the disulfide bond arrangement of human respiratory syncytial virus attachment (g) protein by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | the attachment protein or g protein of the a2 strain of human respiratory syncytial virus (rsv) was digested with trypsin and the resultant peptides separated by reverse-phase high-performance liquid chromatography (hplc). one tryptic peptide produced a mass by matrix-assisted laser desorption/ionization (maldi) time-of-flight (tof) mass spectrometry (ms) corresponding to residues 152-187 with the four cys residues of the ectodomain (residues 173, 176, 182, and 186) in disulfide linkage and abse ... | 1997 | 9194191 |
| transcriptional induction of multiple cytokines by human respiratory syncytial virus requires activation of nf-kappa b and is inhibited by sodium salicylate and aspirin. | infection of the lung epithelial cell line a549 by respiratory syncytial virus (rsv) resulted in the elevated synthesis of multiple cellular cytokines, including a number of interleukins (ils). detailed studies of il-11 induction revealed that it required infection by viable virus and involved a net increase in the steady state level of il-11 mrna. nuclear run-on assays showed a direct effect of rsv on il-11 gene transcription. mutational analysis of the il-11 promoter fused to a reporter lucife ... | 1997 | 9191851 |
| adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cdna allows ecotropic retroviral transduction of established and primary human cells. | the cellular receptors that mediate binding and internalization of retroviruses have recently been identified. the concentration and accessibility of these receptors are critical determinants in accomplishing successful gene transfer with retrovirus-based vectors. murine retroviruses containing ecotropic glycoproteins do not infect human cells since human cells do not express the receptor that binds the ecotropic glycoproteins. to enable human cells to become permissive for ecotropic retrovirus- ... | 1997 | 9188645 |
| genetic analysis of interactions between gag proteins of rous sarcoma virus. | the yeast two-hybrid system was used to characterize homomeric interactions between the gag proteins of rous sarcoma virus (rsv). the rsv gag precursor was found to interact strongly with itself and not with various control proteins. the rsv gag did not interact significantly with gag proteins of a variety of other retroviruses, including murine leukemia viruses and primate lentiviruses. deletion analysis suggested that two nonoverlapping regions are independently sufficient to mediate rsv gag-g ... | 1997 | 9188638 |
| high level of transgene expression in cell cultures and in the mouse by replication-incompetent adenoviruses harboring modified vai genes. | replication-incompetent adenoviruses are currently used in gene therapy trials. most of the work designed to increase the expression from these vectors concerns the modification of cis sequences of the foreign transcription unit, so as to improve the transcription level or the stability of the mrna. in this report, we show that an alternative strategy based on the coexpression of modified vai genes can efficiently increase gene expression both in cell cultures and in animals. the vai rna is synt ... | 1997 | 9188608 |
| analysis of the gene start and gene end signals of human respiratory syncytial virus: quasi-templated initiation at position 1 of the encoded mrna. | the gene start (gs) and gene end (ge) transcription signals of human respiratory syncytial virus (rsv) strain a2 were analyzed in helper-dependent monocistronic and dicistronic minireplicons which were complemented by a standard rsv strain. the gs signal, which is the start site for mrna synthesis, is highly conserved for the first nine genes: 3'-ccccguuua(u/c) (negative sense). this conserved version of the signal was analyzed by "saturation" mutagenesis, in which all 10 positions, as well as o ... | 1997 | 9188557 |
| stable expression of anti-hpv 16 e7-ribozyme in cv-1 cell lines. | the hpv16 (human papilloma virus type 16) e7 gene product, an oncoprotein, has been considered to be involved in the pathogenesis of anogenital cancer, particularly of cervical cancer. in order to evaluate the effect of suppression of the expression of the e7 gene in cv-1 cells by ribozyme, rz523 with a transacting ribozyme targeted to the e7 rna and two processing ribozyme genes at the 5' and 3' flank was cloned into the eukaryotic expression plasmid prep9 under the control of rsv-ltr promoter. ... | 1996 | 9187492 |
| [clinical, therapeutic and developmental aspects of acute bronchiolitis in tunisia]. | fourteen infants with severe acute bronchiolitis were admitted to the intensive care unit (icu) of tunis. this pathology represents 36% of severe bronchopulmonary infections admitted to this icu. their age ranged between 2 and 48 weeks (mean: 15 weeks). eight infants had hypotrophy. two infants had congenital heart disease and one infant had tracheo-bronchomalacia. viruses were found in 6/11 patients. respiratory syncytial virus (rsv) was identified in five patients and an adenovirus in one pati ... | 1996 | 9183423 |
| nosocomial respiratory syncytial virus infections: prevention and control in bone marrow transplant patients. | to assess the effectiveness of a multifaceted infection control strategy in limiting the nosocomial transmission of respiratory syncytial virus (rsv) infection to patients in a bone marrow transplant (bmt) unit. | 1997 | 9181397 |
| expression from cardiomyocyte-specific promoter after adenovirus-mediated gene transfer in vitro and in vivo. | adenoviruses are very attractive vectors for gene transfer into the cardiac muscle; however, their promiscuous tissue tropism, leading to an ectopic expression of the transgene, is a considerable practical limitation. to restrict expression of a reporter gene in cultured cardiomyocytes and in the heart of the rat, we have constructed a recombinant adenovirus (ad-mlc2 beta gal) containing the beta-galactosidase gene under the control of the rat ventricle-specific cardiac myosin light chain 2 (mlc ... | 1997 | 9181118 |
| determinants and kinetics of cytokine expression patterns in lungs of vaccinated mice challenged with respiratory syncytial virus. | the development of a successful respiratory syncytial virus (rsv) vaccine will be advanced by an improved understanding of the pathogenesis of natural disease and vaccine-enhanced illness. using a murine model, we have examined cytokine message expression and cytokine secretion in lungs of mice primed with killed or live antigens and challenged with rsv. stable cytokine mrna expression was achieved if the prime-challenge interval was 2 weeks. the pattern of expression of interleukin-4 (il-4) and ... | 1997 | 9178456 |
| antibodies against chlamydia pneumoniae, cytomegalovirus, enteroviruses and respiratory syncytial virus in patients with polymyalgia rheumatica. | to investigate the association between the onset of polymyalgia rheumatica (pmr) and prior or persistent infection with chlamydia pneumoniae or cytomegalovirus (cmv) (both known to infect the vessel wall) enteroviruses (ev) or respiratory syncytial virus (rsv). | 1997 | 9177926 |
| gene transfer into enteric neurons of the rat small intestine in organ culture using a replication defective recombinant herpes simplex virus type 1 (hsv1) vector, but not recombinant adenovirus vectors. | we have designed a system in which to test gene transfer into gut neurons consisting of an organ culture of neonatal rat small intestine. the tissue was exposed to herpes simplex- and adenovirus-derived vectors: (1) a temperature-sensitive herpes simplex virus-1 (hsv1) vector (tsk-beta gal) containing the lacz gene encoding beta-galactosidase (beta-gal), under the transcriptional control of the hsv1 immediate-early 3 (ie3) promoter; (2) rad35, an e1-/e3- replication-deficient adenovirus expressi ... | 1997 | 9176519 |
| restriction pattern variability of respiratory syncytial virus during three consecutive epidemics in denmark. | a pcr-based assay was used to distinguish between respiratory syncytial virus (rsv) group a and b in order to analyze their prevalence in denmark in three consecutive epidemics during the winters of 1992/93, 1993/94 and 1994/95. a total of 96 rsv strains isolated from hospitalized children were examined, showing alternation of group prevalence. furthermore, the genetic variability of the rsv isolates was illustrated by restriction enzyme analysis of pcr products originating from a part of the f ... | 1997 | 9164473 |
| adjuvants influence the quantitative and qualitative immune response in balb/c mice immunized with respiratory syncytial virus fg subunit vaccine. | the ability of monophosphoryl lipid a (mpl), qs-21 and alum to alter the immunologic response to immunization with respiratory syncytial virus a chimeric fg construct (fg) subunit vaccine was examined in balb/c mice. fg/mpl, fg/alum, and fg/mpl/qs-21 combinations increased non-neutralizing antibody response, while fg/qs-21 did not. fg subunit vaccine with mpl, qs-21, or both had cytokine responses more closely resembling primary infection than fg/alum, with decreased interleukin-4 mrna levels an ... | 1997 | 9160520 |
| production of env-deficient rous sarcoma virus (rsv) early after infection. | production of defective virus particles during the early stage of rous sarcoma virus (rsv) infection of chicken embryo fibroblasts (cef) was examined. rsv harvested 2 days post infection (2pi) had 10 to 30 times lower specific infectivity (focus forming units/unit reverse transcriptase activity) than 5pi harvest. virus particles produced on day 2 contained less env proteins than particles harvested on day 5. the amount of other viral proteins was equal in particles harvested on day 2 and day 5. ... | 1997 | 9158953 |
| [bronchiolitis: revision of 153 cases and comparative study of ribavirin treatment]. | we try in this paper to improve the knowledge of the main epidemiological, clinic, evolutive and therapeutic data in cases of children suffering from bronchiolitis in our medium; we analyze particularly the possible usefulness of ribavirin as a causal treatment. | 1997 | 9157802 |
| antigens of bovine respiratory syncytial virus in peripheral blood lymphocytes of experimentally infected lambs. | eight lambs were experimentally infected with bovine respiratory syncytial virus (rsv) and the distribution of viral antigen in lymphocyte subpopulations studied by flow cytometry. experimental infection with bovine rsv was characterised by significant changes in lymphocyte subpopulations. infection was followed by a significant (p <0.001) reduction in the number of cells expressing the ovcd5 epitope (t cells), due to a fall in the number of cells expressing the ovcd4 epitope (helper) and those ... | 1996 | 9157689 |
| cell-mediated immune responses of lambs to experimental infection with bovine respiratory syncytial virus. | primary infection of lambs with bovine respiratory syncytial virus (rsv) was characterised by the presence of virus-specific cytotoxic cells in the peripheral blood as early as 5 days post-infection. these effector cells lysed virus-infected autologous targets in a self-restricted manner. depletion techniques revealed that cytotoxic activity was largely due to ovcd8+ cells. neutrophils obtained from experimentally infected lambs 5 to 10 days post-inoculation exhibited significant cytotoxic activ ... | 1996 | 9157688 |
| myogenin expression is necessary for commitment to differentiation and is closely related to src tyrosine kinase activity in quail myoblasts transformed with rous sarcoma virus. | quail myoblasts transformed with a temperature-sensitive mutant of rous sarcoma virus (qm-rsv cells) proliferate at 35.5 degrees c, a permissive temperature for rsv, but differentiate at 41 degrees c, a nonpermissive temperature, with the formation of multinucleated myotubes and the synthesis of muscle-specific proteins. tyrosine kinase activity of the src gene product derived from rsv is closely related to regulation of this temperature-dependent differentiation, and the cells obtain commitment ... | 1997 | 9157009 |
| episomal expression of wild-type cftr corrects camp-dependent chloride transport in respiratory epithelial cells. | the isolation of the gene responsible for the cl- ion transport defect in cystic fibrosis (cf) has provided important information about the relationship between the disease pathology and the underlying genetic and biochemical mechanisms. in addition, new areas of investigation and therapy are now possible. most notably, the isolation of the cf gene, the cystic fibrosis transmembrane conductance regulator (cftr) has been led to the development of different gene therapy strategies. to circumvent p ... | 1996 | 9156804 |
| ribavirin therapy in bone marrow transplant recipients with viral respiratory tract infections. | treatment with ribavirin was instituted in 12 allogeneic and one autologous bone marrow transplant (bmt) recipients with proven respiratory syncytial virus (rsv), influenza b virus or parainfluenza virus infections. rsv was diagnosed in six cases, influenza b virus in four and parainfluenza virus in three patients. ribavirin was given orally or intravenously (15-20 mg/kg/day in three divided doses) and in nine cases with the addition of ribavirin inhalations (6 g/day). three patients required ve ... | 1997 | 9156264 |
| treatment of respiratory syncytial virus bronchiolitis: 1995 poll of members of the european society for paediatric infectious diseases. | among the lower respiratory tract infections during infancy requiring hospitalization, respiratory syncytial virus (rsv) bronchiolitis is the most frequent disease entity. nevertheless treatment remains controversial. | 1997 | 9154540 |
| in vitro assembly of virus-like particles with rous sarcoma virus gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles. | retroviruses are unusual in that expression of a single protein, gag, leads to budding of virus-like particles into the extracellular space. we have developed conditions under which virus-like particles are formed spontaneously in vitro from fragments of rous sarcoma virus (rsv) gag protein purified after expression in escherichia coli. the ca-nc fragment of gag was shown previously to assemble into hollow cylinders (s. campbell and v. m. vogt, j. virol. 69:6487-6497, 1995). we have now extended ... | 1997 | 9151833 |
| t cell source of type 1 cytokines determines illness patterns in respiratory syncytial virus-infected mice. | manipulation of the cytokine microenvironment at the time of vaccination can influence immune responses to remote challenge, providing a strategy to study the molecular pathogenesis of respiratory syncytial virus (rsv) vaccine-enhanced disease in the mouse model. although treatment with antibody against il-4 or recombinant il-12 (ril-12) at the time of formalin-inactivated rsv vaccination induced a similar shift in the pattern of cytokine mrna expression upon live virus challenge, anti-il-4 trea ... | 1997 | 9151790 |
| serum eosinophil cationic protein and interleukin-5 in children with bronchial asthma and acute bronchiolitis. | the aim of our study was to evaluate the clinical applicability of serum eosinophil cationic protein (ecp), interleukin-5 (il-5) and total eosinophil counts in childhood asthma and bronchiolitis. these parameters were measured in 44 children aged 12-84 months with moderate and mild asthma during symptomatic and asymptomatic phases of disease. fifteen of the patients were included at the time of admission to hospital due to an acute asthmatic attack, and ten of these were also examined one month ... | 1996 | 9151339 |
| cationic liposome-mediated expression of hiv-regulated luciferase and diphtheria toxin a genes in hela cells infected with or expressing hiv. | hiv-regulated expression of the diphtheria toxin a fragment gene (hiv-dt-a) is a potential gene therapy approach to aids. since cationic liposomes are safe and non-immunogenic for in vivo gene delivery, we examined whether lipofectamine or dmrie reagent could mediate the transfection of hiv-dt-a (ptha43) or the hiv-regulated luciferase gene (pluca43) into hiv-infected or uninfected hela cells. pluca43 was expressed at a 10(3)-fold higher level in hela/lav cells than in uninfected hela cells, whi ... | 1997 | 9150276 |
| induction of protective immunity in rodents by vaccination with a prokaryotically expressed recombinant fusion protein containing a respiratory syncytial virus g protein fragment. | a subunit approach to the development of a respiratory syncytial virus (rsv) vaccine was investigated. it involved the production, in escherichia coli, of an rsv (long) g protein fragment (g2na) as a c-terminal fusion partner to an albumin binding region (bb) of streptococcal protein g. g2na incorporated amino acid residues 130-230 and was specifically recognized by murine anti-rsv-a polyclonal serum. in mice, intraperitoneal immunization with bbg2na induced high anti-rsv-a serum elisa titers an ... | 1997 | 9143271 |
| subgroup specific protection of mice from respiratory syncytial virus infection with peptides encompassing the amino acid region 174-187 from the g glycoprotein: the role of cysteinyl residues in protection. | we identified subgroup specific protective epitopes represented by the amino acid regions 174-187 and 171-187 of the g glycoproteins from respiratory syncytial virus (rsv), subgroups a and b. mice immunized with coupled synthetic peptides corresponding to either the region 174-187 containing a cys186-->ser substitution or to the native region 171-187 were completely resistant to rsv infection but only to the respective virus. the protective activities of the peptides 174-187 were dependent on th ... | 1997 | 9141214 |