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water vapour uptake from subsaturated atmospheres by engorged immature ixodid ticks.contrary to current opinion, fully engorged and detached larvae and nymphs of some ixodid ticks consistently take up substantial amounts of atmospheric water vapour and thereby display their regulative capacity for maintaining water balance in subsaturated air. net uptake of vapour generally begins some days after detachment and the capability persists until shortly after initiation of apolysis, a period which in diapausing specimens may extend up to several months. this was shown for ixodes ric ...19883378463
ticks parasitising the indian buffalo (bubalus bubalis) and their possible role in disease transmission.a total of 13 ixodid tick species, boophilus microplus, haemaphysalis bispinosa, haemaphysalis cornupunctata, haemaphysalis himalaya, heamaphysalis montgomeryi, hyalomma anatolicum anatolicum, hyalomma dromedarii, hyalomma marginatum isaaci, hyalomma (hyalommina) brevipunctata, hyalomma (hyalommina) hussaini, nosomma monstrosum, rhipicephalus haemaphysaloides, and r. turanicus were collected off 424 buffaloes from the northwestern states of india. ten tick species, amblyomma testudinarium, b. mi ...19883369083
treatment of experimentally induced theileria annulata infection in cross-bred calves with buparvaquone.twenty cross-bred (bos taurus x bos indicus) calves, 7-21 days old, were infected by a ground-up tick supernate of hyalomma anatolicum anatolicum infected with the hisar isolate of theileria annulata. six calves acted as untreated controls and they all died of theileriosis within 17 days of infection. the remaining 14 calves were divided into group a and b, each consisting of seven calves. all the calves of groups a and b were treated intramuscularly with buparvaquone (bw 720c) on day 11 post-in ...19883369076
the salivary glands of hyalomma anatolicum anatolicum: nature of salivary gland components and their role in tick attachment and feeding. 19883366541
immunization of neonatal bovines against theileria annulata by an infection and treatment method.sixty three cross-bred (bos taurus x bos indicus) 4-5-day-old calves were divided into 16 groups (a-p). each calf in groups a and b was given ground-up-tick supernate prepared from theileria annulata-infected or non-infected hyalomma anatolicum anatolicum (guts) equivalent to 5 ticks (50 infected acini). groups d and e received infected guts equivalent to 2 ticks (20 infected acini) and groups g and h were given infected guts equivalent to 1 tick (10 infected acini). each calf in groups j, k and ...19873113041
evaluation of purified theileria annulata sporozoite antigen from the tick hyalomma anatolicum anatolicum.rabbits immunized with purified sporozoites from hyalomma anatolicum anatolicum produced antibody to theileria annulata specific antigens as measured by the indirect fluorescent antibody test. nonspecific fluorescence which compounded the interpretation of the test was observed. incorporation of eriochrome black and evans blue as a counterstain in the diluent buffer for the conjugate significantly reduced the background fluorescence.19863097915
stage-specific antigenicity in theileria annulata: a case report.blood from sick cattle in bahrain transmitted piroplasms of theileria annulata to a splenectomized calf. larvae of hyalomma anatolicum anatolicum were infected on the calf and, after moulting, induced clinical theileriosis, associated with numerous schizonts, in the same calf. the animal was cured by specific treatment. antigenic differences thus shown between piroplasms on the one hand, and sporozoites and schizonts on the other hand, were confirmed in the indirect fluorescent antibody test, as ...19863083573
metabolic energy pathways in theileria annulata sporozoites and their significance in sporozoite-bovine lymphocyte interactions in vitro.the existence of metabolic energy pathways has been studied in extracellular t. annulata sporozoites using chemicals known to inhibit specific energy-generating pathways, and their role during invasion of bovine peripheral blood lymphocytes (pbl) by the sporozoites determined in an in vitro system. an inverse relationship was depicted between the dose of various chemicals and the number of t. annulata sporozoites invading pbl: as the concentrations of the inhibitor drugs increased, the number of ...19852986342
variability of powassan virus cultured in tissue explants and organism of hyalomma anatolicum ticks.the variability of powassan virus was studied during successive passages in hyalomma anatolicum ticks or prolonged reproduction in their tissue explants. it had been shown that in the course of tick passages and during reproduction in the explants, pathogenicity of the virus in respect to causing acute disease in mice after peripheral inoculation was decreased, while virus ability to cause death after intracerebral (i.c.) inoculation remained unchanged. in mice infected with the original strain ...20102864833
relationship between the resistance of crossbred cattle to ticks, boophilus microplus (canestrini, 1887) and hyalomma anatolicum anatolicum (koch, 1844).studies were undertaken to determine the effect of repeated pure infestations with boophilus microplus on susceptibility to subsequent pure infestations with hyalomma anatolicum anatolicum, and the effects of pure infestations with both species of tick on susceptibility to a series of mixed infestations. crossbred (bos indicus x bos taurus) calves were infested with boophilus microplus (17 times), h. a. anatolicum (four times), followed by five mixed infestations of b. microplus and h. a. anatol ...19892763448
relative role of male and female hyalomma anatolicum anatolicum ticks in theileria transmission.the relative role of male and female hyalomma anatolicum anatolicum ticks in the epidemiology of theileria transmission was studied by detecting theileria sporozoites in the dissected salivary glands of 568 ticks by the methyl green pyronin staining method. detailed frequency distribution of theileria-positive acini in the salivary glands of the 264 (46.48%) positive ticks from a field collection in haryana indicated that the number of infected salivary acini per positive tick was greater in fem ...19892499092
chemoimmunoprophylaxis against bovine tropical theileriosis in young calves: a comparison between buparvaquone and long-acting oxytetracycline.eighteen seven to 21-day-old crossbred (bos taurus cross bos indicus) calves were allocated to four groups (a to d). groups a and b each consisted of six calves and groups c and d three calves each. each calf in groups a, b and c was inoculated with ground-up tick supernate (guts) equivalent to two infected acini prepared from theileria annulata-infected hyalomma anatolicum anatolicum. each calf in group a was also given a single intramuscular injection of buparvaquone, 2.5 mg kg-1 bodyweight si ...19902382047
immunization of cattle (bos indicus x bos taurus) against hyalomma anatolicum anatolicum using antigens derived from tick salivary gland extracts.studies were conducted on 41 cross-bred (bos taurus x bos indicus) calves which were less than 1 year old. three types of salivary gland antigens (sg ag) viz. sg ag-i (whole), sg ag-ii (supernatant) and sg ag-iii (sediment), prepared from the ixodid tick, hyalomma anatolicum anatolicum, were used for immunizing these calves. the calves were divided into five groups. the first three groups were immunized with sg ag-i, -ii and -iii with freund's complete adjuvant (fca), respectively. the fourth gr ...19902276870
the effects of bovine tick resistance on the susceptibility of hyalomma anatolicum anatolicum to infection with theileria annulata (ankara).two bos taurus calves were made resistant to tick infestation by exposing them to approximately 500 rabbit-reared nymphs of hyalomma anatolicum anatolicum twice at a 2-week interval. these two calves, together with a tick-susceptible control calf, were inoculated with a stabilate of theileria annulata (ankara). patent infection resulted in all three calves. seven-hundred and fifty gerbil-reared nymphs were then applied on each of these calves as well as another tick-susceptible calf that was the ...19902107619
attempted immunisation of crossbred calves (bos taurus x bos indicus) by repeated natural attachment of ticks hyalomma anatolicum anatolicum koch (1844).crossbred calves (bos taurus x bos indicus) were repeatedly infested with the ixodid tick, hyalomma anatolicum anatolicum. the parameters of engorgement, pre-oviposition period, oviposition period, engorged weight, egg batch weight, and per cent hatch of eggs all showed statistically significant differences between first to tenth infestation. only the time to engorgement did not change. it is concluded that repeated infestations of this tick stimulate acquired resistance.19911796526
continuous cell lines from the tick hyalomma anatolicum anatolicum.the establishment of 5 continuous cell lines from embryonic tissues of the tick hyalomma anatolicum anatolicum is reported. each line comprises 2 or more cell types; they are maintained at 28 c and 32 c in l-15/h-lac medium with 20% fetal calf serum, and have been cryopreserved successfully. sustained and consistent growth was achieved only after 12-41 mo in culture.19911779279
effect of temperature on transtadial transmission of theileria annulata in hyalomma anatolicum anatolicum ticks.the effect of temperature on the transtadial transmission of theileria annulata in hyalomma anatolicum anatolicum was studied. variation in temperature (4-40 degrees c) had a significant effect on moulting rate of the ticks and transmission of theilerial parasites from nymphs to resultant adults. the temperatures above 40 degrees c and below 12 degrees c prevented moulting. maximum infection levels were obtained in salivary glands of adult ticks when the infected engorged nymphs were incubated a ...19911763485
effects of immunization of rabbits on establishment, survival, and reproductive biology of the tick hyalomma anatolicum anatolicum.four antigenic preparations, viz. salivary gland antigen (sg ag), whole tick extract antigen (wte ag) and 30,000-g supernatant fraction, and pellet of wte ag (tes ag and tep ag, respectively), were made from partially fed adult female hyalomma anatolicum anatolicum. four groups of 5 rabbits each were immunized with the antigens, and a fifth group was kept as control. following challenge with adult h. a. anatolicum, a significant decrease in engorgement weight and egg mass weight and an increase ...19921738073
transstadial transmission of theileria annulata through common ixodid ticks infesting indian cattle.transstadial transmission of theileria annulata with hyalomma anatolicum anatolicum, h. dromedarii and h. marginatum isaaci, and haemaphysalis bispinosa, rhipicephalus haemaphysaloides haemaphysaloides and boophilus microplus was determined. it was found that the infection was successfully transmitted by h. a. anatolicum from larva to nymph and nymph to adult in all attempts. when larvae were fed on an infected calf the succeeding adults transmitted the infection when the intervening nymphs fed ...19751178216
effects of 60co irradiation on unfed adults and engorged females of the tick hyalomma anatolicum.unfed and engorged adults of the cattle tick hyalomma anatolicum, known to be biological vector of theileria anmulata in india, were exposed to various levels of gamma-radiation from 0-60 000 r, and the effect on engorgement and redproduction was observed. the radiation tolerance limit for unfed adults is 1000 r for engorgement and reproduction. the sterilizing dose for most of the males is 2000 r but the females can tolerate a slightly higher dose. engorged females have a tolerance limit of 10 ...19761083846
effects of 60co irradiation of the early developmental stages of an ixodid tick, hyalomma anatolicum.various developmental stages of hyalomma anatolicum were subjected to a range of doses of 60co radiation from 0-10 000 r, and their subsequent development was observed. some of these ticks were infected with theileria annulata. doses of 2000 r and over prevented all but a very limited development of ticks. after 1000 r a proportion of exposed individuals continued development in a near-normal manner. lower doses had proportionately less effect. 1000 r to ticks infected with t. annulata failed to ...19761083381
immunisation against bovine tropical theileriasis (theileria annulata infection).fully susceptible cross-bred calves, six to nine months of age, were immunised by tick-induced theileria annulata infection treated with chlortetracycline at 16 mg/kg body weight for four, eight or 16 days. the infections were induced with 10 ticks (hyalomma anatolicum anatolicum) or 30 ticks (h dromedarii). the recovered calves were tested for immunity to homologous severe challenge, 50 or 73 days after the first infection. the reaction of the calves to infections was evaluated by noting the pr ...1976968181
[invasiveness and developmental forms of 3 babesia species and piroplasmids (piroplasmida) in the eggs of ixodid ticks].the paper presents the materials on the identification and study of the developmental forms and morphology of b. colchica and p. bigeminum in eggs of hyalomma anatolicum. the description of developmental forms of b. colchica in eggs of boophilus calcaratus is given for the first time. p. bigeminum and p. beliceri were detected in eggs of b. calcaratus and h. anatolicum from the first day of egg production up to the emergence of larvae while b. colchica was found beginning from the 4th-5th day of ...1977896264
possibilities of persistence in new biotopes of ticks imported by birds.survival and development of 6 tick species (hyalomma anatolicum, rhipicephalus sanguineus, haemaphysalis punctata, dermacentor marginatus, d. reticulatus and ixodes ricinus) were studied in 3 groups differing in the range of distribution with respect to the place of importation, as a model for the knowledge of their possible inclusion in the new local biocenosis during transportation by natural hosts.1977852771
[electron microscope studies on developmental stages of theileria parva (theiler, 1904) in the intestine of the tick hyalomma anatolicum excavatum (koch, 1844) (author's transl)].the developmental stages of theileria parva within the intestine of ticks were studied by electron microscopy. on the 2nd-4th day post repletionem (p.r.) the intestine of the ticks contained numerous stages, which were very similar to microgamonts and microgametes of haemosporidia. from these results it seemed likely that gamogony of theileria parva takes place in the intestine of ticks, too. the microgamont-like stages were spear-head-like; they measured about 10.5 mum in length with a maximum ...1976821188
application of the indirect fluorescent antibody test for diagnosis of theileria hirci infection of sheep: using cell culture schizont antigen.the indirect fluorescent antibody test was applied for detection of circulating antibodies in sheep as a result of theileria hirci infections. a schizont antigen was prepared from an in vitro culture suspension of lymphoid cells infected with t. hirci macroschizonts. the peak antibody titre of 1/8, 192 was reached 24 days after the initial antibody rise in the sheep experimentally infected by means of hyalomma anatolicum anatolicum ticks.1976820024
[the antibacterial effect of organs and tissues of hyalomma dromedarii, hyalomma anatolicum and ixodes persulcatus ticks]. 1975815775
[electron microscope studies on development stages of theileria annulata (dschunkowsky, luhs, 1904) in the intestine and haemolymph of hyalomma anatolicum excavatum (koch, 1844) (author's transl)].for the first time developmental stages of theileria within the intestine and haemolymph of ticks were studied by electron microscopy. on the 1st-6th day post repletionem the intestine of the ticks contained humerous tages, which were very similar to microgamonts and microgametes of haemosporidia. later, on the 14th-19th day p.r. ookinete-like stages ere found in the haemolymph of ticks. from these results it seemed likely that gamogony of theileria takes place in the intestine of ticks. in more ...1975814733
[light microscopic studies on the development of theileria annulata (dschunkowsky and luhs, 1904) in hyalomma anatolicum excavatum (koch, 1844). i. the development in the gut of engorged nymphs (author's transl)].a laboratory strain of h. a. excavatum was selected on high susceptibility for t. annulata through several generations. giemsa-stained smears and wet smears of gut and gut content were studied. after engorgement of erythrocytic stages of t. annulata by the nymphs the following development was observed: 1. erythrocytic merozoites developed to slender, spindleshaped "microgamonts" in the gut 24 to 96 hours after repletion (p. repl.). spherical stages with a conspicuous spike developed at the same ...1975814732
studies on the development of theileria annulata dschunskowsky and luhs, 1904 in the tick--hyalomma anatolicum anatolicum koch, 1844.the endogenous development of theileria annulata in the vector tick--hyalomma anatolicum anatolicum has been described. parasites within and outside the erythrocytes were seen in the gut contents of larvae and nymphs immediately after their removal from the infected cattle but not after 24 hours. two bodies resembling the intra-erythrocytic stage of the parasite, lying close to each other were seen near the gut epithelium of a larva on the 4th day of its dropping off the infected host. neither m ...1975813551
on the life cycle of theileria annulata (dschunkowsky and luhs, 1904) in the midgut and hemolymph of hyalomma anatolicum excavatum (koch, 1844). 1975810989
development of nuttallia danii (protozoa: babesidae) within tick salivary glands cultured in vitro.development of the protozoan parasite nuttallia danii was observed in salivary glands which were extirpated from hyalomma anatolicum excavatum nymphs, fed as larvae on infected gerbils and held in organ culture. at 34 c the parasite continued developing in the cultured glands at a rate similar to that observed under normal conditions. development also occurred in culture at 25 degrees c, but at a somewhat slower rate.1978660383
[circadian rhythm of the dropping of engorged larvae in 2 species of ixodid ticks (ixodidae) and its photoperiodic entrainment].daily rhythm of detachment of engorged larvae of ixodes ricinus l. and hyalomma anatolicum koch is regulated by photoperiod. in photoperiodic conditions (16--20 hours of light p. d.) the maximum of detachment in h. anatolicum occurs at the beginning of photophase (the first 4--8 hours), but in i. ricinus it occurs at the second part of photo-phase (12--16 hours after the light is on). the rhythm of detachment is regulated according to the processes of endogenous circadian nature which are mainta ...1979440776
electron microscopic studies of the development of kinetes in theileria annulata dschunkowsky & luhs, 1904 (sporozoa, piroplasmea).gamogony of theileria annulata dschunkowsky & luhs occurs within the intestine of nymphs of the tick hyalomma anatolicum excavatum koch. after the 5th day post repletionem (p.r.) of the ticks spherical and ovoid parasites were found within the intestinal cells. these stages were thought to represent fertilized macrogametes. these underwent a transformation process leading ultimately to the differentiation of a motile stage, the kinete, which leaves the intestinal cells on the 14th-17th day p.r. ...1977407352
the production of nymphs of hyalomma anatolicum anatolicum for experimental infection with theileria annulata.methods are described for the production of nymphs of hyalomma anatolicum anatolicum for later use in transmission experiments with theileria annulata where the timing of the application of nymphs to infected cattle needs to be accurately controlled. larvae are fed on the torsos of rabbits where approximately 98% undergo a two-host feeding cycle. this cycle is interrupted 2 days after the first larvae moult into nymphs by killing the rabbits and the nymphs are then collected with a suction pump. ...1979116404
immunization against bovine tropical theileriosis, using 60co-irradiated infective particles of theileria annulata (dschunkowsky and luhs 1904) derived from ticks.portions of ground supernate material from theileria annulata-infected and prefed adults of the hyalomma anatolicum anatolicum were irridiated by 60co source at 0, 3, 5, 6, 7, or 9 krads. these portions were injected subcutaneously into different groups of cross-bred calves. the calves injected with 0-, 3-, or 5-krad irradiated tick supernate had severe reactions (rectal temperature 39.8 to 41.6 c, two- to fourfold enlargement of prescapular lymph gland, mean peak parasitemia 27.3% to 32.5% infe ...1979112890
infectivity of ground-up tick supernates prepared from theilerai annulata infected hyalomma anatolicum anatolicum.groups of theileria annulata infected hyalomma anatolicum anatolicum adults prefed on a calf for 1 to 6 days were separately ground in tissue culture medium-199, supplemented with bovine albumin powder, fraction-v. supernatant fluid was collected, made up with additional medium, so that each millilitre represented material from 25 ticks, and was injected subcutaneously into groups of cross-bred male calves. the results indicated that ground-up tick supernate (guts) prepared from unfed ticks was ...1979111383
[light microscopic studies on the development of theileria annulata (dschunkowsky and luhs, 1904) in hyalomma anatolicum excavatum (koch, 1844). ii. the development in haemolymph and salivary glands (author's transl)].fully differentiated kinetes, average length 17.6 micrometer, appeared in the haemolymph of engorged nymphs usually 17 to 20 days after repletion. kinetes were observed at first in the salivary glands on day 18 after repletion. the kinetes then transformed into fission bodies of about 10 micrometer in diameter, mainly in type iii alveoli and less frequently in type ii alveoli. the fission bodies grew up to a size of about 20 micrometer after several divisions of their nucleus. at this time the t ...197899896
rapid quantitative assessment of theileria infection in ticks.a simplified method for methyl green pyronin staining is described for theileria parva and t. annulata in whole salivary glands of rhipicephalus appendiculatus and hyalomma anatolicum subspecies respectively. the stain gives results comparable with feulgen staining and can be used after the ticks have been in cold storage for 3 days. there is considerable variability in the rate and intensity of infection of these ticks with theilerial parasites and it is concluded that the method permits large ...197987045
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