Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| pesticidal properties of parthenin (from parthenium hysterophorus) and related compounds. | eleven sesquiterpene lactone derivatives of parthenin (1), obtained from wild feverfew, parthenium hysterophorus, were prepared by chemical and photochemical transformations. the compounds tested were a pyrazoline adduct (2) of parthenin, its cyclopropyl (3) and propenyl (4) derivatives, anhydroparthenin (5), a dihydro-deoxygenated product (6), a formate (7) and its corresponding alcohol (8) and acetate (9), a rearranged product (10), lactone (11) and hemiacetal (12). all these derivatives, alon ... | 2001 | 11455638 |
| insect antifeedant and growth regulating activities of quassinoids from samadera indica. | four quassinoids, indaquassin c (1), samaderins c (2), b (3) and a (4), isolated from the seeds and bark of samadera indica, were tested for insect antifeedant and growth regulatory activities against the tobacco cutworm, spodoptera litura. indaquassin c was the most effective antifeedant. samaderin c increased pupal duration and induced pupal mortality. | 2001 | 11429258 |
| a new continuous cell line from larval hemocytes of spodoptera litura (f.). | a new cell line has been established from larval hemocytes of the moth, s. litura (tobacco cut worm). it took 147 days to form a monolayer and one year for the first 17 passages. at present, the culture is at 86th passage level and is designated niv-su-1095. three cell types could be distinguished, viz. plasmatocytes (53%), prohemocytes (36%) and granular hemocytes (11%). the chromosome number was very high, 74% metaphase cells showed more than 100 chromosomes. the cells could be cryopreserved. ... | 2000 | 11411040 |
| enhancing the efficacy and persistency of spodoptera litura (fab.) nuclear polyhedrosis virus using uv irradiation protectants. | to enhance the field persistency of s. litura nuclear polyhedrosis virus (slnpv), three chemicals viz. cupric ammonium nitrate, tinopal and cupric sulphate were tried as protectants (0.01 mg/ml) against natural sunlight (uv) irradiation. on exposure for 8 hr and subsequent bioassaying (diet surface treatment), it was found that cupric sulphate protected the polyhedrosis inclusion bodies (pibs), recording 95.56% mortality which was statistically at par with unexposed pibs recording 97.78% mortali ... | 2000 | 11395967 |
| highly toxic and broad-spectrum insecticidal bacillus thuringiensis engineered by using the transposon tn917 and protoplast fusion. | the chromosome of the bacillus thuringiensis strain s184 that was toxic against the third instar larvae of spodoptera litura with the lc(50) of 9.74 microg/ml was successfully integrated into two genes of cyt1aa and cry11aa using the transposon tn917, yielding the primary engineered strain tnx. the strain tnx was highly toxic to the third instar larvae of culex pipiens fatigans with the lc(50) of 5.12 ng/ml which was 1.82-fold higher than that of b. thuringiensis subsp. israelensis, but lowly to ... | 2001 | 11391474 |
| cloning and functional expression of a chitinase cdna from the common cutworm, spodoptera litura, using a recombinant baculovirus lacking the virus-encoded chitinase gene. | a chitinase cdna named slchi was cloned from the epidermis of the common cutworm, spodoptera litura, and the enzymatic properties of its recombinant proteins were characterized. the slchi cdna encodes 552 amino-acid residues (aa) including a 19 aa putative signal peptide, with the calculated molecular mass of the putative mature protein 60,152 da. a major transcript of slchi about 2.8 kb was detected in the epidermis only during molting in the last instar larvae, suggesting its involvement in th ... | 2001 | 11267891 |
| acute, sublethal, antifeedant, and synergistic effects of monoterpenoid essential oil compounds on the tobacco cutworm, spodoptera litura (lep., noctuidae). | monoterpenoids (terpenes and biogenically related phenols) commonly found in plant essential oils were tested for acute toxicity via topical application to tobacco cutworms (spodoptera litura fab.). the most toxic among 10 such compounds was thymol (ld(50) = 25.4 microg/larva) from garden thyme, thymus vulgaris. the compounds were then tested for sublethal effects, specifically inhibition of larval growth after topical application of low doses. among 6 compounds tested, an ld(10) dose reduced gr ... | 2001 | 11262018 |
| growth inhibitory and antifeedant activity of extracts from melia dubia to spodoptera litura and helicoverpa armigera larvae. | the growth inhibitory activity and deterrency of melia dubia (meliaceae) extracts to spodoptera litura and helicoverpa armigera were investigated. artificial diet bioassays using neonate larvae of both s. litura and h. armigera indicated that dichloroethane (dce) and methanol (me) extracts of m. dubia inhibited growth in a dose dependent manner. dce and me-5ii fractions also resulted in 50% deterrency at concentrations of 22.5 and 16.8 micrograms/cm2 respectively against s. litura larvae in a le ... | 2000 | 11233088 |
| carbamate and organophosphate resistance in cotton pests in india, 1995 to 1999. | monitoring for organophosphate and carbamate resistance was carried out on five major insect pests of cotton collected from 22 cotton-growing districts across india. resistance was monitored in helicoverpa armigera (hübner) and pectinophora gossypiella (saunders) for the period 1995-1999 and for spodoptera litura (fabricius), earias vittella (fabricius) and bemisia tabaci (gennadius) in a survey conducted during the 1997-98 cropping season. of the 53 field strains of h. armigera, only four were ... | 2001 | 11228586 |
| synthesis and stereostructure-activity relationship of three asymmetric center pyrethroids: 2-methyl-3-phenylcyclopropylmethyl 3-phenoxybenzyl ether and cyanohydrin ester. | 2-methyl-3-phenylcyclopropylmethyl 3-phenoxybenzyl ether 2 and cyanohydrin ester 3, a couple of pyrethroids with three asymmetric centers, were synthesized. of each of the four diastereomers of 2 and 3, only the (1r*,2r*,3r*)-2a and 3a showed significant insecticidal activities. dual sets of enantiomers [(1r,2r,3r)-(-)-2a and (1s,2s,3s)-(+)-2a] and [(1r,2r,3r)-(-)-3a and (1s,2s,3s)-(+)-3a] were synthesized through the asymmetric cyclopropanation using the aratani catalyst. significant separation ... | 2001 | 11197343 |
| insecticidal activity of essential oils to the tobacco cutworm, spodoptera litura. | twenty-one essential oils were tested for insecticidal activity via topical administration to third instar larvae of the tobacco cutworm, spodoptera litura. oils of satureia hortensis, thymus serpyllum and origanum creticum produced >90% larval mortality at 24 h at a dose of 100 microg per larva. the ld(50) value for s. hortensis (48.4 microg) was comparable to that for thymus vulgaris (46.9 microg). the monoterpenoid phenols thymol and carvacrol, major constituents of the oils of thymus and sat ... | 2001 | 11163945 |
| identification of insertion and deletion genes in autographa californica nucleopolyhedrovirus variants isolated from galleria mellonella, spodoptera exigua, spodoptera litura and xestia c-nigrum. | the genomic dna of four autographa californica multinucleocapsid nucleopolyhedrovirus (acmnpv) variants isolated from galleria mellonella, spodoptera exigua, spodoptera litura and xestia c-nigrum was analyzed in comparison with the acmnpv e2 strain. restriction endonuclease analysis revealed a deletion and an insertion in collinear regions of the four variants. polymerase chain reaction analysis indicated that, in the four variants, the deletion occurred in the region corresponding to acmnpv c6 ... | 2000 | 11129632 |
| production of functional hepatocyte growth factor (hgf) in insect cells infected with an hgf-recombinant baculovirus in a serum-free medium. | three insect cell lines, sl-7b cells derived from spodoptera litura, sf9, and high five (hi-5) cells, were used for the production of pro-hepatocyte growth factor (pro-hgf). cells were cultured and then infected with a recombinant hgf-containing baculovirus in a serum-free medium. in sl-7b cells, pro-hgf is synthesized and excreted from the cells and late in infection is converted to a heterodimeric form of hgf even when the cells are grown in serum free medium. conversion of a single-chain form ... | 2000 | 10753438 |
| characterization of the ecdysteroid udp-glucosyltransferase gene of spodoptera litura multinucleocapsid nucleopolyhedrovirus. | the ecdysteroid udp-glucosyltransferase (egt) gene of spodoptera litura multinucleocapsid nucleopolyhedrovirus (spltmnpv), guangzhouisolate was sequenced. the open reading frame of the gene is 1530 nucleotides long, encoding a putative protein of 509 amino acids with mr of 58,500. the 5' noncoding region has a tata box. a polyadenylation signal, aataaa, is contained downstream of the translation stop codon. a putative signal peptide is present at the n-terminus of the protein. homology compariso ... | 1999 | 10719631 |
| green-tissue-specific expression of a reconstructed cry1c gene encoding the active fragment of bacillus thuringiensis delta-endotoxin in haploid tobacco plants conferring resistance to spodoptera litura. | the dna sequence of a truncated cry1c gene encoding the active fragment of bacillus thuringiensis (bt) delta-endotoxin was fully reconstructed by introduction of silent mutations. each of the truncated wild type and the synthetic genes encoding the active fragment of the protoxin was introduced into haploid tobacco plants under the control of the rbcs promoter. to facilitate selection of transgenic tobacco plants with high insecticidal activity, a fusion gene encoding both rat cyp1a1 cytochrome ... | 1999 | 10501003 |
| replication of spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines. | the spodoptera exigua multinucleocapsid nucleopolyhedrovirus (semnpv) was inoculated to eight lepidopteran cell lines derived from spodoptera exigua (se301), spodoptera frugiperda (sf21aeii), spodoptera littoralis (cls-79), spodoptera litura (spli-221), pseudaletia separata (lese-11), trichoplusia ni (hi-5), plutella xylostella (pxl/c) and bombyx mori (bmn4). the productive infection of semnpv was observed only in se301 cells. however, a dot-blot hybridization analysis revealed that semnpv dna r ... | 1998 | 10358729 |
| cloning and expression of apolipophorin-iii from the common cutworm, spodoptera litura. | we have cloned apolipophorin-iii (apolp-iii) cdna from adult fat body of spodoptera litura. the sequence encodes a 188 amino acid polypeptide including a 22 amino acid leader peptide. the circular dichroism spectrum from the purified apolp-iii indicated a considerable content of alpha-helix. sequence alignment showed that s. litura apolp-iii has a relatively high degree of sequence identity with the apolps-iii of lepidopteran, manduca sexta (72%), galleria mellonella (67%), bombyx mori (60%). th ... | 1998 | 9880904 |
| cell growth activity of growth-blocking peptide. | growth-blocking peptide (gbp) is an insect biogenic peptide that retards the development of lepidopteran larvae. cdnas encoding gbp were isolated from three lepidopteran species: pseudaletia separata, mamestra brassicae and spodoptera litura. comparison of these molecules revealed that the gbp coding regions were 70% homologous. in contrast, the upstream regions of the deduced propeptides were only 33% identical. sequence analysis further suggested that gbp shares some structural similarities wi ... | 1998 | 9753606 |
| characterization of the polyhedrin gene of spodoptera litura nuclear polyhedrosis virus. | the polyhedrin gene (polh) of a characteristically distinct spodoptera litura nuclear polyhedrosis virus isolate (simnpv) is identified in the hindiii-f fragment of the viral dna. the nucleotide sequence of the 1057 base pair (bp) region of this fragment contains an open reading frame (orf) without any intervening sequence for coding a polypeptide of 246 amino acids. analysis of the nucleotide sequence and deduced amino acid sequence indicate that this has more than 70% sequence identity to know ... | 1997 | 9311561 |
| envelope protein of parasitic wasp symbiont virus, polydnavirus, protects the wasp eggs from cellular immune reactions by the host insect. | cotesia kariyai polydnavirus (ckpdv) virions are present in the oviducts of c. kariyai wasp and are injected with eggs into the hemocoel of the host armyworm pseudaletia separata larvae during parasitization. evidence that the presence of polydnavirus particles on the surface of the wasp eggs may be essential for prevention of cellular immune reactions by the host hemocytes was obtained by isolating an immunoevasive factor from ckpdv virions. the purified proteinaceous factor protects foreign ma ... | 1997 | 9219544 |
| laboratory screening of different bacillus thuringiensis strains against certain lepidopteran pests and subsequent field evaluation on the pod boring pest complex of pigeonpea (cajanus cajan). | laboratory evaluation of different bacillus thuringiensis subspecies revealed that b. thuringiensis subsp. kurstaki (ncim 2514) at 10(8) spores/ml concentration caused more than 85% mortality to the neonate larvae of the lepidopteran insects spodoptera litura (f.) and phthorimaea operculella (z.). this strain at 10(10) spores/ml concentration was effective against the major lepidopteran pests comprising the pod boring complex of pigeonpea (cajanus cajan), viz. helicoverpa armigera (h.) and exela ... | 1997 | 9195006 |
| expression of lacz reporter gene under the control of the polyhedrin promoter of spodoptera litura nuclear polyhedrosis virus. | promoter function of the putative polyhedrin-encoding gene (polh) of spodoptera litura nuclear polyhedrosis virus (s1mnpv) was determined by transferring it to the autographa californica nuclear polyhedrosis virus (acmnpv) through the acnpv polh based vector, pvl1393. three transfer vectors pcbt2, pcbt3 and pcbt4 were constructed by substituting the promoter and the neighbouring sequences of acnpv in pvl1393 by that of s1npv. the escherichia coli lacz gene was placed downstream from the s1npv po ... | 1997 | 9185860 |
| establishment of a cell line from spodoptera litura (lepidoptera: noctuidae) and replication of s. litura nuclear polyhedrosis virus in vitro. | a new cell line, designated ibl-slo1a, was established from pupal ovaries of the tobacco cutworm spodoptera litura. cells were grown in the tnm-fh insect cell culture medium supplemented with 10% fetal bovine serum. the subculture of cells was initiated with 1-3 x 10(5) cells/ml, a split ratio about 1:10. the growth curve of the cells fit the model of exponential growth (y = e-0.6750 + 0.0317x r2 = 0. 98), the population doubling time during logarithmic growth at 28 degrees c was 21.9 hr. the nu ... | 1997 | 9028921 |
| insecticidal activity of the protein encoded by the cryv gene of bacillus thuringiensis kurstaki ina-02. | a new host specificity was discovered with the insecticidal protein encoded by the cryv gene. the cryv gene was cloned from the bacillus thuringiensis kurstaki ina-02 strain, which was selected among a number of b. thuringiensis isolates because of its high activity against spodoptera litura. analyses by polymerase chain reaction (pcr) revealed that ina-02 contained the cryia(a) and cryv genes. since no spodoptera activity was observed with b. thuringiensis sotto, which contained only cryia(a), ... | 1996 | 8867460 |
| restriction endonuclease analysis of a spodoptera litura nuclear polyhedrosis virus (npv) isolate. | the dna of a nuclear polyhedrosis virus (npv) propagated in the larvae of s. litura was analysed with alui, apai, bamhi, bglii, bsteii, bstni, clai, ecori, ecorv, hindiii, hinfi, kpni, mspi, psti, pvuii, sali, sau3ai, smai, taqi, xbai, and xhoi. the unique restriction endonuclease profiles of the virus indicate it to be a distinct npv isolate. the size of the viral genome was estimated to 132 kbp; the genome was mapped with hindiii and psti. | 1996 | 8799322 |
| replication of a mamestra brassicae nuclear polyhedrosis virus in a newly established mamestra brassicae cell line. | a continuous cell line, designated mbl-3, was newly established from minced neonate larvae of the cabbage armyworm, mamestra brassicae. this new cell line is heteroploid, containing triploid cells predominantly at the frequency of about 50%. doubling time of the cell population is 33 hrs. the akutsu isolate of a mamestra brassicae nuclear polyhedrosis virus (mbnpv) was examined for replication in 16 continuous cell lines, including the cell line mbl-3, from seven lepidoptera species: mamestra br ... | 1995 | 8578995 |
| immunologically unrelated heliothis sp. and spodoptera sp. midgut membrane-proteins bind bacillus thuringiensis cryia(b) delta-endotoxin. | the bacillus thuringiensis toxins, cryia(a), cryia(b), cryia(c) and cryic were used in a ligand-blot assay to detect specific toxin-binding proteins in the brush-border membranes of heliothis virescens, helicoverpa zea, spodoptera littoralis, spodoptera exigua and spodoptera litura. while cryia(a) and cryia(b) always recognize the same protein(s) in a given species, cryia(c) and cryic were found to bind to other proteins. polyclonal antibodies directed against the cryia(b) binding protein of h. ... | 1993 | 8383036 |
| genome organization of the dna-binding protein gene region of cryptophlebia leucotreta granulosis virus is closely related to that of nuclear polyhedrosis viruses. | the nucleotide sequence and genomic organization of a conserved genome region within the ecori g fragment of cryptophlebia leucotreta granulosis virus (clgv) is presented. five open reading frames (orfs) were identified which were homologous to those of autographa californica nuclear polyhedrosis virus (acmnpv), located upstream of the helicase gene (p143) at 63.6 to 65.6 map units. the orfs of clgv and acmnpv share nucleotide sequence homologies of about 47 to 53% and are very similarly arrange ... | 1994 | 8021613 |
| intracellular signal transduction of pban action in lepidopteran insects: inhibition of sex pheromone production by compactin, an hmg coa reductase inhibitor. | pheromone biosynthesis activating neuropeptide (pban) regulates sex pheromone production in the pheromone glands of many species of female moths. in order to probe the biochemical steps as well as underlying mechanisms regulated by pban, we have tested the effect of chemicals on sex pheromone production by using an in vitro assay. among the chemicals we tested here, compactin, a specific 3-hydroxy-3-methylglutaryl coenzyme a (hmg coa) reductase inhibitor, clearly inhibited the pheromone biosynth ... | 1995 | 7480881 |
| effects of caffeine and theophylline on the fecundity of four lepidopteran species. | effects of secondary plant metabolites (caffeine and theophylline on reproduction of lepidopteran species were studied using bombyx mori, spodoptera litura, danaus chrysippus and catopsilia crocale as test organisms. reproductive potential (ovary length and egg number) was significantly reduced in the lepidopterans receiving caffeine/theophylline treated leaves in comparison to the control. protein and energy contents in the egg of the moths showed remarkable decrease with increasing concentrati ... | 1985 | 3850026 |
| trehalase inhibitors, validoxylamine a and related compounds as insecticides. | validoxylamine a showed a potent inhibitory activity against insect trehalase in a competitive manner with a ki value of 4.3 x 10(-10) m. the other validoxylamines and validamycins also exhibited the activity in vitro. injection of these compounds to young last instar larvae of the tobacco cutworm, spodoptera litura, elicited the morphological abnormality followed by death after the cessation of feeding. validoxylamine a showed 100% mortality at a dose of 10 micrograms/ last instar larva. | 1990 | 2380116 |
| characteristically distinct isolates of the nuclear polyhedrosis virus from spodoptera litura. | more than 100 isolates were plaque-purified to examine the genetic variations in four wild stocks of spodoptera litura nuclear polyhedrosis virus (npv) collected in japan. these isolates were characterized by their in vitro host range in three established insect cell lines, growth characteristics, polyhedral protein, dna restriction endonuclease pattern and dna hybridization. the isolates were separated into four distinct groups: (i) isolates corresponding to autographa californica npv, (ii and ... | 1990 | 2254753 |
| role of insects as inhalant allergens in bronchial asthma with special reference to the clinical characteristics of patients. | the whole body extracts (wbes) of 13 common insects from delhi, namely musca domestica (house fly); sitophilus oryzae (rice weevil); callosobrochous maculatus (pulse beetle); anopheles stephensi, culex quinquefasciatus and aedes aegypti (mosquitoes); blattella germanica and periplaneta americana (cockroaches: male, female and nymph); spodoptera litura and heliothis armigera (moths), were prepared to evaluate their allergenic significance in patients with allergic bronchial asthma. intradermal (i ... | 1990 | 2253082 |
| host range expansion by recombination of the baculoviruses bombyx mori nuclear polyhedrosis virus and autographa californica nuclear polyhedrosis virus. | the mechanisms of host specificity of nuclear polyhedrosis viruses (npvs) (baculoviridae) were analyzed after coinfection of bombyx mori npv (bmnpv) and one of four distinct groups of spodoptera litura npv (slnpv), including an autographa californica npv (acnpv) variant (s. maeda, y. mukohara, and a. kondo, j. gen. virol. 71:2631-2639, 1990), into various lepidopteran cell lines. replication of bmnpv in nonpermissive cells (tn-386, sf-21, and cls-79) was induced by coinfection with acnpv but not ... | 1991 | 2041087 |
| two new isolates of bacillus thuringiensis pathogenic to spodoptera litura. | both the standard bacillus thuringiensis kurstaki (hd-1) and the formulated commercial product resulted from this strain have shown limited pathogenicity against the tobacco cutworm (spodoptera litura). however, two new isolates of bacillus thuringiensis (k-2074 and k-2178) isolated from taiwan have been identified through an active screening program to be highly pathogenic against the tobacco cutworm. in this paper, we present results of characterization and the pathogenicity of these two new i ... | 1991 | 1885923 |
| insecticidal activity of inhibitors of polyamine biosynthesis on spodoptera litura f. larvae. | the inhibitors of polyamine (pa) biosynthesis such as alpha-difluoromethylornithine (dfmo), methylglyoxal bis (guanylhydrazone) (mgbg) and bis (cyclohexylammonium) sulphate (bcha) have been used to protect crop plants from pathogenic fungi. in this communication the insecticidal activity of these inhibitors on tobacco caterpillar, s. litura has been reported. all the inhibitors exhibited insecticidal activity; mgbg being more effective than others. the results suggest, for the first time, a poss ... | 1991 | 1794877 |
| construction of chimeric insecticidal proteins between the 130-kda and 135-kda proteins of bacillus thuringiensis subsp. aizawai for analysis of structure-function relationship. | eight chimeric insecticidal protein (ip) genes were constructed between the 130-kda and 135-kda ip genes of bacillus thuringiensis subsp. aizawai, and expressed in escherichia coli jm103 cells. the characterization of the produced chimeric ips indicated that the variable region (vr1) in the amino-terminal half of the ips is responsible for the insecticidal activity against larvae of spodoptera litura and plutella xylostella. the carboxy-terminal half of vr1 was important for the formation of the ... | 1990 | 1368534 |
| insecticidal activity and processing in larval gut juices of genetically engineered 130-kda proteins of bacillus thuringiensis subsp. aizawai. | the 130-kda insecticidal protein (ip) of bacillus thuringiensis subsp. aizawai is proteolytically processed in the gut juice of susceptible insect larvae to yield an insecticidally active 60-kda fragment. twenty-seven mutant ip genes with the replacement of codons for arg and lys with codons for gln in the active fragment and its adjacent regions of the 130-kda ip were constructed by site-directed mutagenesis and expressed in escherichia coli cells. the produced mutant ips at arg87, arg131, arg1 ... | 1992 | 1368122 |
| processing of delta-endotoxin from bacillus thuringiensis subsp. kurstaki hd-1 and hd-73 by gut juices of various insect larvae. | midgut juices were prepared from adoxophyes sp., smaller tea tortrix (stt); bombyx mori, silkworm (sw); spodoptera litura, common cutworm (ccw); plutella xylostella, diamondback moth (dbm); and musca domestica, housefly (hf) and immobilized onto sepharose 4b. delta-endotoxins (icps) from bacillus thuringiensis subsp. kurstaki hd-1 and hd-73 were digested by these immobilized gut juice proteases. all gut juices tested derived relatively proteolytic resistant cores from icp. the molecular sizes of ... | 1992 | 1328398 |
| isolation and partial characterization of binding proteins for immobilized delta endotoxin from solubilized brush border membrane vesicles of the silkworm, bombyx mori, and the common cutworm, spodoptera litura. | 1. brush border membrane vesicles (bbmv) were prepared from the bombyx mori, and spodoptera litura, midguts. bbmv was solubilized. 2. activated delta endotoxin from bacillus thuringiensis were immobilized. 3. solubilized bbmv proteins were applied to the toxin column and the proteins bound were analyzed by sds-page. 4. in the case of b. mori m(r) = 220,000, 150,000 and 130,000 and in the case of s. litura 160,000 bands were detected. 5. the bindings were inhibited by n-acetyl galactosamine and m ... | 1992 | 1323444 |
| observations on the nature of resistance in spodoptera litura (f.) (noctuidae: lepidoptera) to infection by bacillus thuringiensis berliner. | 1975 | 1218923 |