Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| characterization and bioactivity of hepcidin-2 in zebrafish: dependence of antibacterial activity upon disulfide bridges. | hepcidin is an antimicrobial peptide and iron-regulatory molecule with highly conserved disulfide bridges among vertebrates, but structural insights into the function in fish remains largely missing. we demonstrate here that recombinant hepcidin-2 from zebrafish is capable of inhibiting the growth of the gram-negative bacteria escherichia coli and vibrio anguillarum, and the gram-positive bacteria staphylococcus aureus and bacillus subtilis with minimum inhibitory concentrations (mics) of 18, 15 ... | 2014 | 24787654 |
| identification of 21 novel immune-type receptors in miiuy croaker and expression pattern of three typical inhibitory members. | novel immune-type receptor (nitr) genes belong to the immunoglobulin superfamily and are encoded by clusters of multigene families. nitrs encode type i transmembrane proteins and are only found in teleosts. in the current study, total 21 nitr genes are identified from miiuy croaker (miichthys miiuy) and named as mmnitr1 to mmnitr21. miiuy croaker nitr genes that encoded one or two extracellular immunoglobulin (ig) domains, a transmembrane (tm) region, an immunoreceptor tyrosine-based inhibitor m ... | 2014 | 24704213 |
| host-induced increase in larval sea bass mortality in a gnotobiotic challenge test with vibrio anguillarum. | vibrio anguillarum is the major cause of haemorrhagic septicaemia, vibriosis, which is a severe disease affecting marine fish. in this work, it was found that the mortality of gnotobiotic sea bass larvae challenged with v. anguillarum was dependent on the number of dead fish in the vials at the moment of challenge. based on this finding, the effect of dead hosts (homogenised sea bass larvae or brine shrimp) on the virulence of v. anguillarum towards sea bass larvae was further investigated. addi ... | 2014 | 24695234 |
| a new fibrinogen-related protein from argopecten irradians (aifrep-2) with broad recognition spectrum and bacteria agglutination activity. | fibrinogen-related proteins (freps) are a kind of pattern recognition receptors (prrs) containing fibrinogen-like (fbg) domains, and they play curial roles in the innate immune response. in the present study, a new frep protein was identified from bay scallop argopecten irradians (designated as aifrep-2). the full-length cdna of aifrep-2 was of 1299 bp with an open reading frame of 762 bp encoding a polypeptide of 253 amino acids, including a signal sequence and an fbg domain. the fbg domain in ... | 2014 | 24680667 |
| modification of a synthetic lps-binding domain of anti-lipopolysaccharide factor from shrimp reveals strong structure-activity relationship in their antimicrobial characteristics. | anti-lipopolysaccharide factor (alf) is a small protein with broad-spectrum antimicrobial activities and certain antiviral property. its putative lipopolysaccharide (lps) binding domain was deduced to be important for its activities. however, there is still no report revealing how the structure of the lps-binding domain affects its biological function until now. in the present study, we designed and synthesized a peptide corresponding to the lps-binding domain of alf from the chinese shrimp (des ... | 2014 | 24661926 |
| the effect of vaccinating turbot broodstocks on the maternal immunity transfer to offspring immunity. | the study was designed to explore the effect of vaccinating turbot broodstocks on the immunity of offsprings. the expression of igm, c3, tgf-β1, il-1β, transferrin, lysozyme and their concentrations were detected respectively with quantitative real-time pcr and elisa kits in offsprings at different stages from both naive and vaccinated brood stocks. the survival rate of offsprings in pathogen challenge experiment and their pathogen inhibition rate were also detected. the results shown that the m ... | 2014 | 24657725 |
| identification and expression analysis of lipopolysaccharide-induced tnf-alpha factor gene in chinese mitten crab eriocheir sinensis. | lipopolysaccharide-induced tnf-alpha factor (litaf) is an important transcription factor in transcriptional regulation of tnf-alpha and other cytokines. here we identified a full-length litaf homolog cdna, termed eslitaf, that contains a 71 bp 5'-untranslated sequence, an open reading frame consisting of 381 bp, and a 208 bp 3'-untranslated sequence in chinese mitten crab (eriocheir sinensis), an economically important freshwater crustacean species in china. eslitaf protein possesses a conserved ... | 2014 | 24657317 |
| a galectin from the kuruma shrimp (marsupenaeus japonicus) functions as an opsonin and promotes bacterial clearance from hemolymph. | galectins are a lectin family characterized by a conserved sequence motif in the carbohydrate recognition domain, which preferential binds to galactosyl moieties. however, few studies about the biological roles of galectins in invertebrates have been reported except for the galectin (cvgal1) from the eastern oyster crassostrea virginica. furthermore, galectins have been described in only a few crustacean species, and no functional studies have been reported so far. in this study, we identified a ... | 2014 | 24618590 |
| vibriophages and their interactions with the fish pathogen vibrio anguillarum. | vibrio anguillarum is an important pathogen in aquaculture, responsible for the disease vibriosis in many fish and invertebrate species. disease control by antibiotics is a concern due to potential development and spread of antibiotic resistance. the use of bacteriophages to control the pathogen may offer a non-antibiotic-based approach to reduce vibriosis. a detailed understanding of the phage-host interaction is needed to evaluate the potential of phages to control the pathogen. in this study, ... | 2014 | 24610858 |
| the specifically enhanced cellular immune responses in pacific oyster (crassostrea gigas) against secondary challenge with vibrio splendidus. | the increasing experimental evidences suggest that there are some forms of specific acquired immunity in invertebrates, but the underlying mechanism is not fully understood. in the present study, pacific oyster (crassostrea gigas) stimulated primarily by heat-killed vibrio splendidus displayed stronger immune responses at cellular and molecular levels when they encountered the secondary challenge of live v. splendidus. the total hemocyte counts (thc) increased significantly after the primary sti ... | 2014 | 24607288 |
| a novel lipopolysaccharide-binding protein (lbp) gene from sweetfish plecoglossus altivelis: molecular characterization and its role in the immune response of monocytes/macrophages. | lipopolysaccharide-binding protein (lbp) belongs to the lipid transfer/lbp (lt-lbp) family, and plays a crucial role in the recognition of bacterial components that modulate cellular signals in phagocytic cells. although several lbps have been identified in teleosts, the effects of lbp homologs on teleost phagocytic cells are still obscure. here, we report the cloning a novel full-length cdna sequence of lbp-like protein (palbp) gene from sweetfish, plecoglossus altivelis. the palbp cdna encoded ... | 2014 | 24594008 |
| gene cloning and expression analysis of irf1 in half-smooth tongue sole (cynoglossus semilaevis). | interferon regulatory factor 1 (irf1) was known to play key roles in antiviral defense in several species, and some other important biological processes. in this report, full length cdna of irf1 from cynoglossus semilaevis (csirf1) was identified. it was of 1,455 bp, containing a 5' utr of 104 bp, a 3' utr of 541 bp with a poly (a) tail and an orf of 810 bp encoding a putative protein of 269 amino acids. the putative csirf1protein contained one conserved irf domain (1-113aa), and two low complex ... | 2014 | 24584575 |
| upconversion fluorescent strip sensor for rapid determination of vibrio anguillarum. | here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on nayf4:yb,er nanoparticles (nps) and the lateral flow immunochromatographic assay (lfia). carboxyl-modified β-nayf4:yb,er nps were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant ucnps-antibody conjugates probes were used in lfia and served as signal vehicles for the fluorescent reporters. v. anguillarum was used as a model an ... | 2014 | 24577708 |
| a genome scan for quantitative trait loci associated with vibrio anguillarum infection resistance in japanese flounder (paralichthys olivaceus) by bulked segregant analysis. | a recent genetic linkage map was employed to detect quantitative trait loci (qtls) associated with vibrio anguillarum resistance in japanese flounder. an f1 family established and challenged with v. anguillarum in 2009 was used for qtl mapping. of the 221 simple sequence repeat (ssr) markers used to detect polymorphisms in the parents of f1, 170 were confirmed to be polymorphic. the average distance between the markers was 10.6 cm. equal amounts of genomic dna from 15 fry that died early and fro ... | 2014 | 24562474 |
| th17-like immune response in fish mucosal tissues after administration of live attenuated vibrio anguillarum via different vaccination routes. | this study aimed to investigate the protective mucosal immunity elicited by live attenuated vibrio anguillarum in fish. zebrafish were immunized by bath or injection way, and undertook bath challenge at 28 days post vaccination. the results implied that bath vaccination was the better delivery route for inducing the protective immunity against bath challenge in zebrafish. the expressions of genes related to th1, th2 and th17 cells were measured in the mucosal tissues of vaccinated and challenged ... | 2014 | 24561130 |
| l-type lectin from the kuruma shrimp marsupenaeus japonicus promotes hemocyte phagocytosis. | l-type lectins (ltls) contain a luminal carbohydrate recognition domain, which exhibits homology to leguminous lectins. these type i membrane proteins are involved in the early secretory pathway of animals, and have functions in glycoprotein sorting, trafficking and targeting. recent studies suggest that ltls may be involved in immune responses in vertebrates, but no functional studies have been reported. this study reports an ltl, designated as mjltl1, from the kuruma shrimp marsupenaeus japoni ... | 2014 | 24508102 |
| deep transcriptome sequencing of pecten maximus hemocytes: a genomic resource for bivalve immunology. | pecten maximus, the king scallop, is a bivalve species with important commercial value for both fisheries and aquaculture, traditionally consumed in several european countries. major problems in larval rearing, however, still limit hatchery-based seed production. high mortalities during early larval stages, likely related to bacterial pathogens, represent the most relevant bottleneck. to address this issue, understanding host defense mechanisms against microbes is extremely important. in this st ... | 2014 | 24486903 |
| passive protective effect of chicken egg yolk immunoglobulins against experimental vibrio anguillarum infection in ayu (plecoglossus altivelis). | oral administration of chicken egg yolk immunoglobulins (igy) has attracted much attention as a means for controlling infectious diseases caused by microorganisms. this study evaluated the protective effect of igy against vibrio anguillarum infection in ayu, plecoglossus altivelis. igy was isolated from egg yolks laid by hens initially immunized with formalin-inactivated v. anguillarum. lower mortality of ayu was observed in groups treated with anti-v. anguillarum igy (avigy), compared with thos ... | 2014 | 24486630 |
| molecular characterization, immune responsive expression and functional analysis of qm, a putative tumor suppressor gene from the pacific white shrimp, litopenaeus vannamei. | the qm, firstly identified as a putative tumor suppressor gene from human, has been confirmed to possess varieties of functions in a range of organisms. in the present study, the cdna that encodes a 220-amino-acid qm protein with calculated molecular mass of 25.5 kda and isoelectric point of 10.07 was characterized from the pacific white shrimp litopenaeus vannamei. analysis of the deduced amino acid sequence of lvqm revealed that it contained a series of conserved functional motifs. quantitativ ... | 2014 | 24444825 |
| staphylococcus warneri, a resident skin commensal of rainbow trout (oncorhynchus mykiss) with pathobiont characteristics. | commensal microorganisms live in association with the mucosal surfaces of all vertebrates. the skin of teleost fish is known to harbor commensals. in this study we report for the first time the presence of an intracellular gram positive bacteria, staphylococcus warneri that resides in the skin epidermis of rainbow trout (oncorhynchus mykiss). s. warneri was isolated from healthy hatchery trout skin epithelial cells. in situ hybridization confirmed the intracellular nature of the bacterium. skin ... | 2014 | 24438987 |
| identification of two secreted ferritin subunits involved in immune defense of yesso scallop patinopecten yessoensis. | as an important iron storage protein, ferritin plays a crucial role in the iron-withholding defense system. in this study, two secreted ferritin subunits (pyfers1 and pyfers2) were identified from the yesso scallop, patinopecten yessoensis. the complete dna sequences of the two ferritins are 7101 and 5359 bp, consisting of seven and five exons, respectively. the full-length cdnas of pyfers1 and pyfers2 are 960 and 956 bp in length, encoding 228 and 220 amino acids, respectively. they have typica ... | 2014 | 24434645 |
| rnai knock-down of shrimp litopenaeus vannamei toll gene and immune deficiency gene reveals their difference in regulating antimicrobial peptides transcription. | nf-κb dependent antimicrobial peptides (amps) are of critical importance in protecting insects or mammals from microorganisms infection. however, we still do not make clear signaling pathways in regulating amps expression in shrimps. in this study, rnai approach was used to study differences between toll signaling pathway and immune deficiency signaling pathway in regulating the transcription of nf-κb dependent amps post bacteria challenge. results showed that the transcription level of anti-lip ... | 2014 | 24434197 |
| specific dna probes for the identification of the fish pathogen, renibacterium salmoninarum. | to obtain specific dna probes for the identification of the fish pathogen, renibacterium salmoninarum, a discriminatory recombinant dna library was constructed using selective fragments of the bacterial genome. three renibacterial clones, pmam29, pmam46 and pmam77, containing 149, 73, and 154 bp respectively, were isolated and characterized. the specificity of the probes was confirmed by dot-blot and southern hybridization analyses. bacterial hybridization experiments revealed that pmam29 discri ... | 1994 | 24420936 |
| molecular characterization of leap-2 cdna in common carp (cyprinus carpio l.) and the differential expression upon a vibrio anguillarum stimulus; indications for a significant immune role in skin. | leap-2 is a cysteine-rich cationic antimicrobial peptide (amp) playing an important role in host innate immune system. leap-2 genes have been identified from higher vertebrates and several fish species. here we report the cloning and identification of two leap-2 cdna sequences from the liver of common carp (cyprinus carpio l.). the leap-2a cdna was 1325 bp long and contained an orf of 279 bp encoding a protein of 92 amino acids. the leap-2b cdna was 608 bp long and contained an orf of 276 bp enc ... | 2014 | 24418455 |
| antibacterial activity of serine protease inhibitor 1 from kuruma shrimp marsupenaeus japonicus. | serine protease inhibitors (serpins) are a large family of protease inhibitors involved in many critical biological processes such as blood coagulation, fibrinolysis, programmed cell death, development, and innate immunity. we identified mjserp1, a serpin in the kuruma shrimp marsupenaeus japonicus. the mjserp1 cdna has a 1239 bp open reading frame (orf) that encodes a 412-amino acid protein with a 23 aa signal peptide and a classic serpin domain. mjserp1 has a calculated molecular mass of 46.3 ... | 2014 | 24412215 |
| the responsive expression of a chitinase gene in the ridgetail white prawn exopalaemon carinicauda against vibrio anguillarum and wssv challenge. | chitinases are essential enzymes for crustaceans and participates in several biological processes, such as nutrient digestion, morphogenesis, pathogenesis, and pathogen defense. in the present study, the full-length cdna of chi (named ecchi) was cloned from the hemocytes of ridgetail white prawn exopalaemon carinicauda by rapid amplification of cdna ends methods. the full-length cdna of ecchi was 1,319 bp, including contains a 5'-untranslated region (utr) of 42 bp, 3'-utr of 101 bp with a poly ( ... | 2014 | 24408604 |
| molecular cloning and expression of irak-4, il-17 and i-κb genes in haliotis rufescens challenged with vibrio anguillarum. | the candidate genes interleukin-1 receptor associated kinase 4 (irak-4), interleukin 17 (il-17) and inhibitor of nf-κb (i-κb) were cloned and evaluated in californian abalone (haliotis rufescens) hemocytes in response to vibrio anguillarum. molecular characterization evidenced that hri-κb has a full cdna sequence of 3027 bp with an encoding region of 401 amino acids (aa), hrirak-4 comprised 1969 bp that encoded for 516 aa, and hr-il17 had a full sequence of 806 bp encoding for 165 aa. qpcr analy ... | 2014 | 24398261 |
| gene structure, immune response and evolution: comparative analysis of three 2-cys peroxiredoxin members of miiuy croaker, miichthys miiuy. | peroxiredoxin family was a superfamily of selenium independent peroxidases. it was divided into six subtypes: prx1-4 (typical 2-cys), prx5 (atypical 2-cys) and prx6 (1-cys). this study reports the isolation and characterization three 2-cys peroxiredoxin members of full cdna and genomic clones from miiuy croaker (miichthys miiuy). the genetic structure analysis showed that the c-terminal catalytic cys positioned within gevcpaxw. this sequence was different between prx3 and prx4, but was conservat ... | 2014 | 24378678 |
| genomic organization, single nucleotide polymorphism and functional characterization of natural killer enhancing factor (nkef-a) in miichthys miiuy. | peroxiredoxin (prx) play vital parts in oxidative stress belonging to a cellular antioxidant protein family. natural killer enhancing factor (nkef) is a member of the prx family, which is newly defined. in addition to antioxidant activity, nkef also can protect dna from oxidative damage. in order to study immune defense mechanism of nkef in teleost, nkef-a gene of miiuy croaker (miichthys miiuy) was cloned and characterized. the genomic organization containing one non-coding exon, five coding ex ... | 2014 | 24352703 |
| a low-density lipoprotein receptor-related protein (lrp)-like molecule identified from chlamys farreri participated in immune response against bacterial infection. | low-density lipoprotein receptor-related protein (lrp) is a group of important endocytic receptors contributing to binding ligands and maintaining internal environment. in the present study, an lrp-like molecule was identified from zhikong scallop chlamys farreri (cflpr), and its mrna expression profiles, tissue location, and immunology activities were analyzed to explore its possible function in the innate immune system. the orf of cflrp was of 1971 bp encoding a polypeptide of 656 amino acids ... | 2014 | 24345370 |
| characterization and expression of the cxcr1 and cxcr4 in miiuy croaker and evolutionary analysis shows the strong positive selection pressures imposed in mammal cxcr1. | the innate immune system can recognize non-self, danger signals, and pathogen associated molecular patterns and provides a first line of antimicrobial host defense. therefore, it plays an instructive role and is pretty important in vertebrates. in innate immune responses, cxcrs act as the main receptors of cxc chemokines and play a vital role in host defense and inflammation. in present study, we cloned two cdna molecules of cxcr1 and cxcr4 in miichthys miiuy (miiuy croaker). in these two genes, ... | 2014 | 24333436 |
| toll-like receptor 22 of gilthead seabream, sparus aurata: molecular cloning, expression profiles and post-transcriptional regulation. | tlr22 is a fish-specific tlr that recognizes dsrnas. in the present study, a tlr22 homologue gene from gilthead seabream (sbtlr22) was identified and characterized. the full coding sequence contained a single open-reading frame of 2895 nucleotides encoding a predicted protein of 964 amino acids in length. its 3'-utr was relatively long, 1380 nucleotides, and contained three au-rich sequences frequently associated with mrna instability. functional studies showed that the sbtlr22 transcript had a ... | 2014 | 24333435 |
| identification and characterization of cynoglossus semilaevis microrna response to vibrio anguillarum infection through high-throughput sequencing. | micrornas (mirna) play key regulatory roles in diverse biological processes. cynoglossus semilaevis is an important commercial mariculture fish species in china. to identify mirnas and investigate immune-related mirnas of c. semilaevis, we performed high-throughput sequencing on three small rna libraries prepared from c. semilaevis immune tissues (liver, head kidney, spleen, and intestine). one library was prepared under normal conditions (control, cg); two were prepared during vibrio anguillaru ... | 2014 | 24296438 |
| characterization of plp, a phosphatidylcholine-specific phospholipase and hemolysin of vibrio anguillarum. | vibrio anguillarum is the causative agent of vibriosis in fish. several extracellular proteins secreted by v. anguillarum have been shown to contribute to virulence. while two hemolysin gene clusters, vah1-plp and rtxachbde, have been previously identified and described, the activities of the protein encoded by the plp gene were not known. here we describe the biochemical activities of the plp-encoded protein and its role in pathogenesis. | 2013 | 24279474 |
| oxasetin from lophiostoma sp. of the baltic sea: identification, in silico binding mode prediction and antibacterial evaluation against fish pathogenic bacteria. | because of the evolving resistance of microorganisms against existing antibiotics, there is an increasing need for new ones, not only in human, but also in veterinary medicine. the dichloromethane extract of a fungal strain of the genus lophiostoma, isolated from driftwood collected from the coast of the baltic sea, displayed antibacterial activity against some fish pathogenic bacteria. ergosterol epoxide (1), cerebroside c (2) and oxasetin (3) were isolated from the extract and structurally elu ... | 2013 | 24273851 |
| a new type antimicrobial peptide astacidin functions in antibacterial immune response in red swamp crayfish procambarus clarkii. | a new antibacterial peptide called astacidin was characterized from hemocytes of red swamp crayfish procambarus clarkii, and designated as pcast. the full-length cdna of pcast contained 828 nucleotides with a polyadenylation sequence and a poly-a tail. pcast encoded a peptide of 43 amino acids, with a signal peptide of 23 amino acids. the mature peptide contained 20 amino acids, among which four were proline/arginine amino acids. similarity analysis showed that pcast shared high identity with as ... | 2014 | 24263004 |
| the protein expression profile in hepatopancreas of scallop chlamys farreri under heat stress and vibrio anguillarum challenge. | heat stress and pathogen infection have been considered as the main causes for mass mortality of cultured scallops during summer. in the present study, the expression profiles of proteins in the hepatopancreas of scallop chlamys farreri were examined to reveal the possible mechanisms of physiological responses of scallop beneath heat stress and bacterial infection. an earlier occurred and higher mortality was observed in the scallops from combination treated group (28 °c and an injection of vibr ... | 2014 | 24262301 |
| spalf4: a newly identified anti-lipopolysaccharide factor from the mud crab scylla paramamosain with broad spectrum antimicrobial activity. | anti-lipopolysaccharide factors (alfs) are antimicrobial peptides with binding and neutralizing activities to lipopolysaccharide (lps) in crustaceans. this study identified and characterized a novel alf homolog (spalf4) from the mud crab scylla paramamosain. the complete cdna of spalf4 had 756 bp with a 381 bp open reading frame encoding a protein with 126 aa. the deduced protein contained a signal peptide and a lps-binding domain. spalf4 shared the highest identity with ptalf5 at amino acid lev ... | 2014 | 24239582 |
| molecular cloning and multifunctional characterization of grim-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (scophthalmus maximus). | grim-19 (gene associated with retinoid-interferon-induced mortality 19), a novel cell death regulatory gene, plays important roles in cell apoptosis, embryogenesis, mitochondrial respiratory chain and immune response. to date, little information is known about fish grim-19 characteristics except orange-spotted grouper (epinephelus coioides). here a new grim-19 gene is identified and characterized from turbot (scophthalmus maximus), an economic marine fish in china and europe. briefly, turbot gri ... | 2014 | 24239557 |
| molecular cloning and expression of a c-type lectin gene from venerupis philippinarum. | c-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the micro-invaders. in the present study, a c-type lectin gene (denoted as vpctl) was identified from venerupis philippinarum by expressed sequence tag and rapid amplification of cdna ends approaches. the full-length cdna of vpctl consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. the deduced amin ... | 2014 | 24213925 |
| identification, characterization and functional analysis of a serine protease inhibitor (lvserpin) from the pacific white shrimp, litopenaeus vannamei. | as important arthropod immune responses, prophenoloxidase (propo) activation and toll pathway initiation are mediated by serine proteinase cascades and regulated by serpins. herein, a serine protease inhibitor (lvserpin), encoding for 415 amino acids with calculated molecular weight of 46,639 da and isoelectric point of 7.03 was characterized from the pacific white shrimp litopenaeus vannamei. multiple sequence alignment revealed that lvserpin shared the highest similarity with penaeus monodon s ... | 2014 | 24211341 |
| ecvig, a novel grouper immune-gene associated with antiviral activity against nnv infection. | vhsv-induced genes (vigs) were first identified in rainbow trout (oncorhynchus mykiss) and subsequently isolated in a variety of fish. recent studies have shown that most vigs have immunological functions against pathogenic infections. however, most research has focused on vig1, such that our present understanding of these genes in other fish species remains limited. this study isolated a homologue of the uncharacterized o. mykiss vig-b319 (ecvig) from orange-spotted grouper (epinephelus coioide ... | 2014 | 24211340 |
| involvement of pacific oyster cgpgrp-s1s in bacterial recognition, agglutination and granulocyte degranulation. | peptidoglycan recognition protein (pgrp) recognizes invading bacteria through their peptidoglycans (pgn), a component of the bacterial cell wall. insect pgrps contribute to effective immune systems as inducers of other host defense responses, while this function has not been reported from pgrp of bivalves. in this study, recombinant cgpgrp-s1s (rcgpgrp-s1s), produced in the mantle and the gill, was synthesized and used to elucidate the immunological function of cgpgrp-s1s. rcgpgrp-s1s bound spec ... | 2014 | 24201133 |
| molecular responses of calreticulin gene to vibrio anguillarum and wssv challenge in the ridgetail white prawn exopalaemon carinicauda. | calreticulin (crt), as a highly conserved endoplasmic reticulum luminal resident protein, plays important roles in ca(2+) homeostasis, molecular chaperoning and response to viral infection. in this study, a full-length cdna of crt (designated eccrt) was cloned from hemocytes of the ridgetail white prawn exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of eccrt was 1725 bp, which contains a 5'-untranslated region (utr) of 57 bp, 3'-utr of 4 ... | 2014 | 24188748 |
| the detection of fish pathogenvibrio anguillarum in water and fish using a species-specific dna probe combined with membrane filtration. | the marine bacteriumvibrio anguillarum causes disease in fish worldwide and is particularly devastating in aquaculture. little is known about the ecology ofv. anguillarum in the environment and how this may relate to the pathogenicity of this organism. combining membrane filtration and a species-specific dna probe, culturablev. anguillarum cells were detected in water from three habitats and in chinook salmon (onchorynchus tshawytscha) tissue samples. results show that different marine habitats ... | 1994 | 24186556 |
| probiotics-pathogen interactions elicit differential regulation of cutaneous immune responses in epidermal cells of atlantic cod gadus morhua. | little is known on the cutaneous immune responses during probiotics-pathogen interactions in fish. thus, this study employed atlantic cod primary epidermal (ep) cell cultures as a model to understand this interaction. the probiotics-pathogen interactions in the ep cell cultures were elucidated using vibrio anguillarum 2133 (va) as the pathogen and two host-derived bacteria (gp21 and gp12) as the probiotics. there was a regional size difference on the ep cells; i.e., ep cells from the dorsal regi ... | 2014 | 24176817 |
| immune-related functions of the hivep gene family in east african cichlid fishes. | immune-related genes are often characterized by adaptive protein evolution. selection on immune genes can be particularly strong when hosts encounter novel parasites, for instance, after the colonization of a new habitat or upon the exploitation of vacant ecological niches in an adaptive radiation. we examined a set of new candidate immune genes in east african cichlid fishes. more specifically, we studied the signatures of selection in five paralogs of the human immunodeficiency virus type i en ... | 2013 | 24142922 |
| cdna cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn exopalaemon carinicauda. | peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species. in this study, a full-length of peroxiredoxin 5 (designated ecprx5) cdna was cloned from the ridgetail white prawn exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of the ecprx5 was of 827 bp, containing a 5' untranslated region (utr) of 14 bp, a 3' utr of 228 bp with a poly (a) tail, a ... | 2013 | 24141991 |
| oral administration of formalin killed vibrio anguillarum cells improves growth and protection against challenge with vibrio harveyi in banana shrimp. | larval rearing in hatcheries and highly intensive grow-out culture practices followed in shrimp production systems favour the growth of potential pathogenic bacterial loads. this study reports the efficacy of formalin-killed vibrio bacterin on growth, survival and protection to challenge with virulent vibrio harveyi and vibrio anguillarum in juveniles of banana shrimp fenneropenaeus merguiensis. postlarvae 15 (0·24 ± 0·01 g) were administered orally in different concentrations of bacterial prepa ... | 2014 | 24138117 |
| a farnesoic acid o-methyltransferase (famet) from exopalaemon carinicauda is responsive to vibrio anguillarum and wssv challenge. | methyl farnesoate (mf), an analogue of the insect juvenile hormone iii, is believed to play important roles in the regulation of the growth and reproductive development in crustaceans. farnesoic acid o-methyltransferase (famet) is the key enzyme in the juvenile hormone biosynthetic pathway, involved in the conversion of farnesoic acid (fa) to mf in the final step of mf synthesis. in this study, a famet cdna (named ecfamet) was cloned from the hemocytes of ridgetail white prawn exopalaemon carini ... | 2014 | 24136172 |
| antibacterial activity of the lipopetides produced by bacillus amyloliquefaciens m1 against multidrug-resistant vibrio spp. isolated from diseased marine animals. | in this work, the antibacterial activity of the lipopeptides produced by bacillus amyloliquefaciens m1 was examined against multidrug-resistant vibrio spp. and shewanella aquimarina isolated from diseased marine animals. a new and cheap medium which contained 1.0 % soybean powder, 1.5 % wheat flour, ph 7.0 was developed. a crude surfactant concentration of 0.28 mg/ml was obtained after 18 h of 10-l fermentation and diameter of the clear zone on the plate seeded with vibrio anguillarum was 34 mm. ... | 2014 | 24132666 |
| effect of multiple infections with white spot syndrome virus and vibrio anguillarum on pacific white shrimp litopenaeus vannamei (l.): mortality and viral replication. | multiple infections are commonly found in practical shrimp culture and may cause more serious consequences than infections by one pathogen only. therefore, this study was conducted to evaluate the effect of multiple infections with white spot syndrome virus (wssv) and vibrio anguillarum on pacific white shrimp litopenaeus vannamei (l.), mortality, wssv replication in vivo and host immune response. in the wssv single-infection group (wssv load, 2 × 10(2) copies μl(-1)), mean cumulative mortality ... | 2014 | 24127689 |
| a fibrinogen-related protein (tffrep2) gene involving in the immune response of trachidermus fasciatus against vibrio anguillarum. | fibrinogen-related proteins play important roles in the immune responses. we have obtained a cdna encoding a novel fibrinogen-related protein from roughskin sculpin trachidermus fasciatus (t. fasciatus) and named it as tffrep2. the n and c terminus of tffrep2 contain a putative 21-amino acid signal peptide and a typical 217-amino acid fibrinogen-like domain, which is conserved in all fibrinogen-related proteins. tffrep2 has three glycosylation sites and two potential calcium-binding sites that a ... | 2013 | 24091062 |
| production of homozygous transgenic rainbow trout with enhanced disease resistance. | previous studies conducted in our laboratory showed that transgenic medaka expressing cecropin b transgenes exhibited resistant characteristic to fish bacterial pathogens, pseudomonas fluorescens and vibrio anguillarum. to confirm whether antimicrobial peptide gene will also exhibit anti-bacterial and anti-viral characteristics in aquaculture important fish species, we produced transgenic rainbow trout expressing cecropin p1 or a synthetic cecropin b analog, cf-17, transgene by sperm-mediated ge ... | 2013 | 24085608 |
| complete genome sequence of vibrio anguillarum m3, a serotype o1 strain isolated from japanese flounder in china. | vibrio anguillarum is an important bacterial pathogen that causes vibriosis in marine fish. we present the complete genome sequence of v. anguillarum m3, a serotype o1 clinical strain isolated from japanese flounder (paralichthys olivaceus) in shandong, china. | 2013 | 24072867 |
| comparison of protein expression profiles of the hepatopancreas in fenneropenaeus chinensis challenged with heat-inactivated vibrio anguillarum and white spot syndrome virus. | fenneropenaeus chinensis (chinese shrimp) culture industry, like other penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. to better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. firstly, the soluble hepatopancreas protein samples in adult chinese shrimp among control, heat-inactivated vibrio-challenged and white spot syndrome virus-infected groups were separated ... | 2014 | 24057166 |
| differential metabolic responses of clam ruditapes philippinarum to vibrio anguillarum and vibrio splendidus challenges. | clam ruditapes philippinarum is one of the important marine aquaculture species in north china. however, pathogens can often cause diseases and lead to massive mortalities and economic losses of clam. in this work, we compared the metabolic responses induced by vibrio anguillarum and vibrio splendidus challenges towards hepatopancreas of clam using nmr-based metabolomics. metabolic responses suggested that both v. anguillarum and v. splendidus induced disturbances in energy metabolism and osmoti ... | 2013 | 24056279 |
| proteomic and metabolomic responses in hepatopancreas of mytilus galloprovincialis challenged by micrococcus luteus and vibrio anguillarum. | the outbreak of pathogens can induce diseases and lead to massive mortalities of aquaculture animals including fish, mollusk and shrimp. in this work, the responses induced by micrococcus luteus and vibrio anguillarum were investigated in hepatopancreas of mussel mytilus galloprovincialis using proteomics and metabolomics. metabolic biomarkers demonstrated that m. luteus and v. anguillarum injections could induce osmotic stress and disturbance in energy metabolism. and the uniquely and more mark ... | 2013 | 24056185 |
| vibrio anguillarum bacterin uptake via the gills of japanese flounder and subsequent immune responses. | the mucosal surfaces of fish allow for the introduction of foreign substances, including antigens, from the surrounding environment. in this study, uptake of vibrio anguillarum j-o-3 serotype bacterin by japanese flounder, and the subsequent immune responses were investigated. immunohistochemistry revealed that the bacterin was taken up through the epithelial cells of gills. the transcription levels of inflammatory cytokines such as interleukin (il)-1β, il-6 and tumor necrosis factor α were sign ... | 2013 | 24035752 |
| application of relative real-time pcr to detect differential expression of virulence genes in vibrio anguillarum under standard and stressed growth conditions. | in this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in vibrio anguillarum. we observed the in vitro responses of two mediterranean strains of v. anguillarum to temperature, nacl and iron concentration changes. we monitored growth performance and gene transcription levels by comparing the results obtained under stressed conditions (temperatures of 5 °c, 15 °c and 37 °c; nacl concentrations of 3% and 5%; and iron depletion and excess) with those o ... | 2014 | 24033758 |
| transcriptome profiling reveals th17-like immune responses induced in zebrafish bath-vaccinated with a live attenuated vibrio anguillarum. | a candidate vaccine, live attenuated vibrio anguillarum developed in our laboratory could prevent vibriosis of fish resulted from v. anguillarum and v. alginolyticus. to elucidate the molecular mechanisms underlying the vaccine protection, we used microarray technology to compare the spleen transcriptomes of bath-vaccinated and unvaccinated zebrafish at 28 days post vaccination. | 2013 | 24023910 |
| clinical vibrio anguillarum infection in lumpsucker cyclopterus lumpus in scotland. | 2013 | 24008994 | |
| a c-type lectin (aictl-3) from bay scallop argopecten irradians with mannose/galactose binding ability to bind various bacteria. | c-type lectins are a family of ca(2+)-dependent carbohydrate-binding proteins playing crucial roles in innate immunity of vertebrates and invertebrates. in the present study, the cdna of a c-type lectin with one carbohydrate-recognition domain (crd) of 127 amino acids was cloned from bay scallop argopecten irradians (designated aictl-3) by rapid amplification of cdna end (race) techniques based on expressed sequence tag (est) analysis. the mrna transcripts of aictl-3 could be detected in all the ... | 2013 | 24008017 |
| molecular cloning and transcriptional regulation of an allograft inflammatory factor-1 (aif-1) in zhikong scallop chlamys farreri. | the allograft inflammatory factor-1 (aif-1) is one of the key factors associated with inflammatory response. in the present study, the full-length cdna of aif-1 was identified from zhikong scallop chlamys farreri (named as cfaif-1) by est (expressed sequence tag) analysis and race (rapid-amplification of cdna ends) approaches. the cdna of cfaif-1 consisted of a 5-terminal untranslated region (utr) of 58 bp, a 3-utr of 607 bp with a poly (a) tail, and an open reading frame (orf) of 468 bp encodin ... | 2013 | 24001778 |
| a c-type lectin could selectively facilitate bacteria clearance in red swamp crayfish, procambarus clarkii. | c-type lectins function as pattern recognition receptors and play important roles in the innate immune system of crustaceans. in this study, we reported a new ctl gene (designated as pclec5) from red swamp crayfish, procambarus clarkii. pclec5 was mainly distributed in hepatopancreas, gills and intestine, and the pclec5 transcripts were up-regulated in all the three tissues after challenge with bacteria vibrio anguillarum. for further functional analyses, pclec5 was recombinantly expressed in es ... | 2013 | 23989040 |
| identification and functional studies of akirin, a potential positive nuclear factor of nf-κb signaling pathways in the pacific white shrimp, litopenaeus vannamei. | as conserved nuclear factors, akirins play critical roles in regulating antimicrobial peptides (amps) transcription downstream of nf-κb dependent signaling pathways in insects and mammals. however, no any functional studies was reported in penaeid shrimp. the identification and functional analysis of akirin in the pacific white shrimp, litopenaeus vannamei were made in this research. the 833 nucleotides cdna of litopenaeus vannamei akirin (lvakirin) was obtained with an open reading frame of 639 ... | 2013 | 23962743 |
| does virulence assessment of vibrio anguillarum using sea bass (dicentrarchus labrax) larvae correspond with genotypic and phenotypic characterization? | vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus vibrio such as vibrio (listonella) anguillarum. despite a lot of research efforts, the virulence factors and mechanism of v. anguillarum are still insufficiently known, in part because of the lack of standardized virulence assays. | 2013 | 23936439 |
| molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (stat2) from turbot, scophthalmus maximus. | signal transducer and activator of transcription 2 (stat2) is an important molecule involved in the type i interferon signalling pathway. to date, little stat2 homologue is available in fish except atlantic salmon and goldfish. in this paper, stat2 was firstly cloned and characterized from turbot, a marine flatfish with high economic value. briefly, turbot stat2 cdna is 3206 bp in length encoding a predicted protein of 793 amino acids. the phylogenetic tree shows that turbot stat2 protein shared ... | 2013 | 23933433 |
| the involvement of pdgf/vegf related factor in regulation of immune and neuroendocrine in chinese mitten crab eriocheir sinensis. | members of the platelet-derived growth factor/vascular endothelial growth factor (pdgf/vegf) family have been implicated in cell proliferation, cell differentiation, and cell migration, vascular development, angiogenesis and neural development. in the present study, a novel pdgf/vegf related factor gene was cloned and identified in chinese mitten crab eriocheir sinensis (designated as espvf1). the full-length cdna of espvf1 was of 1173 bp, consisting a 5' untranslated region (utr) of 54 bp, a 3' ... | 2013 | 23933264 |
| recombinant tnfα as oral vaccine adjuvant protects european sea bass against vibriosis: insights into the role of the ccl25/ccr9 axis. | vibrio anguillarum is the main causative agent of vibriosis in cultured sea bass. unfortunately, available vaccines against this disease do not achieve the desired protection. in this study, to accomplish uptake, processing, and presentation of luminal antigens, a commercial sea bass oral vaccine against v. anguillarum was improved with the addition of recombinant fish-self tumor necrosis factor α (rtnfα), as adjuvant. to explore mechanisms, systemic and local responses were analyzed through ser ... | 2013 | 23932985 |
| effectiveness of probiotic phaeobacter bacteria grown in biofilters against vibrio anguillarum infections in the rearing of turbot (psetta maxima) larvae. | the rearing environment of first-feeding turbot larvae, usually with high larvae densities and organic matter concentrations, may promote the growth of opportunistic pathogenic vibrionaceae bacteria, compromising the survival of the larvae. the aim of this study was to assess the effectiveness of the biofilm-forming probiotic phaeobacter 27-4 strain grown on a ceramic biofilter (probiofilter) in preventing vibrio anguillarum infections in turbot larvae. in seawater with added microalgae and main ... | 2013 | 23917459 |
| differential response of two ferritin subunit genes (vpfer1 and vpfer2) from venerupis philippinarum following pathogen and heavy metals challenge. | as a principal extracellular iron storage molecule, ferritin plays an important role in the iron-withholding strategy of innate immunity and detoxification system. in this study, we cloned and characterized another ferritin from venerupis philippinarum (designated as vpfer2), in addition to one previously reported (vpfer1). vpfer2 possessed all the conserved features critical for the fundamental structure and function of ferritin h subunit. vpfer1 and vpfer2 mrna were both found to be most abund ... | 2013 | 23891591 |
| molecular characterization and expression analysis of toll-like receptor 21 cdna from paralichthys olivaceus. | toll-like receptor (tlr) is believed to play crucial role in host defense of pathogenic microbes in innate immune system. in the present study, the full-length cdna of paralichthys olivaceus toll-like receptor 21 (po-tlr21) was cloned by homology cloning and rapid amplification of cdna ends (race) technique. the po-tlr21 cdna sequence was 3687 bp, containing an open reading frame of 2922 bp encoding 973 amino acids. tmhmm and smart program analysis indicated that protein contained one transmembr ... | 2013 | 23880453 |
| functional analysis of domain of unknown function (duf) 1943, duf1944 and von willebrand factor type d domain (vwd) in vitellogenin2 in zebrafish. | vitellogenin (vg), the precursor of egg-yolk proteins in all oviparous organisms, shares a similar domain structure combination. in most cases, vg contains the vitellogenin_n domain, the domain of unknown function (duf) 1943, and the von willebrand factor type d domain (vwd), which are present in different forms of vg from both vertebrates and invertebrates. occasionally, a duf1944 domain is also present in between duf1943 and vwd in some vg proteins of vertebrates. recent studies have shown tha ... | 2013 | 23867754 |
| molecular characterization of hepcidin gene in common carp (cyprinus carpio l.) and its expression pattern responding to bacterial challenge. | hepcidin is a cysteine-rich cationic antimicrobial peptide (amp), which plays an important role in host innate immune system and iron regulation. a great many of hepcidin genes have been identified from vertebrates, including various fish species. here we report the cloning and identification of a hepcidin cdna from the liver of common carp (cyprinus carpio l.). the full-length cdna of the common carp hepcidin was 647 bp, which contained an orf of 276 bp encoding a prepropeptide of 91 amino acid ... | 2013 | 23851290 |
| characterization of an immune deficiency homolog (imd) in shrimp (fenneropenaeus chinensis) and crayfish (procambarus clarkii). | the immune deficiency (imd) signal pathway mediates immunity against gram-negative bacteria in drosophila. recent studies show that the imd pathway also involves in antiviral innate immune responses. the functions of the pathway in crustacean immunity are largely unknown. in this paper, two imds (fcimd and pcimd), one of the key elements of the imd pathway, were identified from chinese white shrimp fenneropenaeus chinensis and red swamp crayfish procambarus clarkii. both proteins have a death do ... | 2013 | 23850721 |
| concholepas concholepas ferritin h-like subunit (ccfer): molecular characterization and single nucleotide polymorphism associated to innate immune response. | ferritin has been identified as the principal protein of iron storage and iron detoxification, playing a pivotal role for the cellular homeostasis in living organisms. however, recent studies in marine invertebrates have suggested its association with innate immune system. in the present study, one ferritin subunit was identified from the gastropod concholepas concholepas (ccfer), which was fully characterized by rapid amplification of cdna ends technique. simultaneously, a challenge test was pe ... | 2013 | 23838046 |
| h-ns is a negative regulator of the two hemolysin/cytotoxin gene clusters in vibrio anguillarum. | hemolysins produced by vibrio anguillarum have been implicated in the development of hemorrhagic septicemia during vibriosis, a fatal fish disease. previously, two hemolysin gene clusters responsible for the hemolysis and cytotoxicity of v. anguillarum were identified: the vah1-plp gene cluster and the rtxachbde gene cluster. in this study, we identified the hns gene, which encodes the h-ns protein and acts as a negative regulator of both gene clusters. the v. anguillarum h-ns protein shares str ... | 2013 | 23836825 |
| a novel c-type lysozyme from mytilus galloprovincialis: insight into innate immunity and molecular evolution of invertebrate c-type lysozymes. | a c-type lysozyme (named as mgclyz) gene was cloned from the mussel mytilus galloprovincialis. blast analysis indicated that mgclyz was a salivary c-type lysozyme which was mainly found in insects. the nucleotide sequence of mgclyz was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. the deduced mature peptide of mgclyz was of a calculated molecular weight of 14.4 kd and a theoretical isoelectric point (pi) of 8.08. evolution ... | 2013 | 23818979 |
| characterization of the ccr3 and ccr9 genes in miiuy croaker and different selection pressures imposed on different domains between mammals and teleosts. | the innate immune system can recognize non-self through pattern recognition receptors and provides a first line of antimicrobial host defense. thus innate immunity plays a very important role in resistance against major bacterial disease in vertebrates. in the innate immune responses, the chemokine receptors act as the main receptors of chemokines which are released at the sites of infection, inflammation and injury. in this study, the miichthys miiuy ccr3 and ccr9 genes were cloned and characte ... | 2013 | 23817141 |
| disruption of cell-to-cell signaling does not abolish the antagonism of phaeobacter gallaeciensis toward the fish pathogen vibrio anguillarum in algal systems. | quorum sensing (qs) regulates phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that qs is not important for antagonism in algal cultures. qs mutants reduced vibrio anguillarum to the same extent as the wild type. consequently, a combination of probiotic phaeobacter and qs inhibitors is a feasible strategy for aquaculture disease control. | 2013 | 23811510 |
| modulation of haemocyte phagocytic and antibacterial activity by alpha-adrenergic receptor in scallop chlamys farreri. | the adrenergic receptors are a class of g protein-coupled receptors, through which norepinephrine and epinephrine trigger the second messenger to modulate the immune response in immunocytes of vertebrate. in the present study, a gene coding the homologue of α-adrenergic receptor was identified from scallop chlamys farreri (designated cfαar). its deduced protein comprised 318 amino acids, and contained a conserved 7tm_1 domain. after cfαar protein was expressed in the hek293 cells, the stimulatio ... | 2013 | 23811406 |
| two rab gtpases, esrab-1 and esrab-3, involved in anti-bacterial response of chinese mitten crab eriocheir sinensis. | rab gtpase is essential for the control of intracellular membrane trafficking in all eukaryotic cells and further affects the ability of phagocytic cells to scavenge pathogen. in the present study, the cdnas for two crab rab proteins (esrab-1 and esrab-3) were identified from the chinese mitten crab eriocheir sinensis by rapid amplification of cdna ends (race) approaches and expressed sequence tag (est) analysis. the full-length cdnas of esrab-1 and esrab-3 were of 892 bp and 965 bp with orfs of ... | 2013 | 23791860 |
| expression profiles of selenium dependent glutathione peroxidase and glutathione s-transferase from exopalaemon carinicauda in response to vibrio anguillarum and wssv challenge. | a selenium-dependent glutathione peroxidase cdna was obtained from the ridgetail white prawn exopalaemon carinicauda (ecgpx) by rapid amplification of cdna ends (race) methods. the full-length cdna of ecgpx was 946 bp, which contains a 5'-untranslated region (utr) of 105 bp, 3'-utr of 280 bp with a poly (a) tail, an open reading frame (orf) of 561 bp, encoding a 186 amino-acid polypeptide with the predicted molecular weight of 21.35 kda and estimated isoelectric point of 7.65. it involves a puta ... | 2013 | 23770349 |
| immune responses evoked by infection with vibrio anguillarum in zebrafish bath-vaccinated with a live attenuated strain. | live attenuated vaccines are a promising application to control bacterial fish diseases. a live attenuated vibrio anguillarum vaccine candidate was established in our laboratory to protect fish against vibriosis. to elucidate the mechanism of immunoprotection, it is necessary to compare the different immune responses to infection between vaccinated and non-vaccinated fish. in this study, the expression levels of pathogen-specific antibodies and immune-related genes upon challenge at 28 days post ... | 2013 | 23768660 |
| colonization of fish skin is vital for vibrio anguillarum to cause disease. | vibrio anguillarum causes a fatal haemorrhagic septicaemia in marine fish. during initial stages of infection, host surfaces are colonized; however, few virulence factors required for colonization of the host are identified. in this study, in vivo bioluminescent imaging was used to analyse directly the colonization of the whole rainbow trout animal by v. anguillarum. the wild type rapidly colonized both the skin and the intestines by 24 h; however, the bacterial numbers on the skin were signific ... | 2010 | 23766008 |
| anguibactin- versus vanchrobactin-mediated iron uptake in vibrio anguillarum: evolution and ecology of a fish pathogen. | vibrio anguillarum is a marine bacterium that is present in many marine aquatic environments and that is the main cause of vibriosis in diverse wild and cultured fish species. two siderophore-mediated iron uptake systems have been described in v. anguillarum. one, mediated by the siderophore anguibactin, is encoded by the pjm1-type plasmids and is restricted to serotype o1 strains. the second one is mediated by the vanchrobactin siderophore and is widespread in many strains belonging to differen ... | 2010 | 23765994 |
| molecular characterization and expression analysis of four cathepsin l genes in the razor clam, sinonovacula constricta. | cathepsin l (ctsl) is a lysosomal cysteine protease involved in immune responses in vertebrates. however, few studies exist regarding the role of cathepsin l in bivalves. in this study, we isolated and characterized four cathepsin l genes from the razor clam sinonovacula constricta, referred to as ctsl1, ctsl2, ctsl3 and ctsl4. these four genes contained typical papain-like cysteine protease structure and enzyme activity sites with erwnin-like and gnfd-like motifs in the proregion domain and an ... | 2013 | 23765116 |
| genetic analysis of vibriosis and viral nervous necrosis resistance in atlantic cod (gadus morhua l.) using a cure model. | the aim of this study was to investigate whether observed time-until-death of atlantic cod (gadus morhua l.) juveniles in separate challenge tests with vibrio anguillarum (causes vibriosis) and nodavirus [causes viral nervous necrosis (vnn)] are due to differences in susceptibility (whether at risk or not) or increased endurance (individual hazard, given that the animal is susceptible) using a cure mixture (cure) model with gibbs sampling. observed time-until-death, prepared as sequential binary ... | 2013 | 23736060 |
| cloning and identification of four mu-type glutathione s-transferases from the giant freshwater prawn macrobrachium rosenbergii. | glutathione s-transferases (gsts) are essential components of the cellular detoxification system because of their capability to protect organisms against the toxicity of reactive oxygen species (ross). four different gsts (mrmugst1-mrmugst4) showing similarities with mu-type gsts were cloned from the hepatopancreas of macrobrachium rosenbergii. these four gsts have 219, 216, 218 and 219 amino acids in length, respectively. mrmugst1-mrmugst4 proteins all have a g-site in the n-terminus and an h-s ... | 2013 | 23727284 |
| responses of mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics. | pathogens can cause diseases and lead to massive mortalities of aquaculture animals and substantial economic loss. in this work, we studied the responses induced by micrococcus luteus and vibrio anguillarum in gill of mussel mytilus galloprovincialis at protein and metabolite levels. metabolic biomarkers (e.g., amino acids, betaine, atp) suggested that both m. luteus and v. anguillarum induced disturbances in energy metabolism and osmotic regulation. the unique and some more remarkably altered m ... | 2013 | 23711471 |
| differentially expressed proteins in the skin mucus of atlantic cod (gadus morhua) upon natural infection with vibrio anguillarum. | vibriosis caused by v. anguillarum is a commonly encountered disease in atlantic cod farms and several studies indicate that the initiation of infection occurs after the attachment of the pathogen to the mucosal surfaces (gut, skin and gills) of fish. therefore it is necessary to investigate the role of different mucosal components in fish upon v. anguillarum infection. the present study has two parts; in the first part we analyzed the differential expression of skin mucus proteins from atlantic ... | 2013 | 23672475 |
| a single whey acidic protein domain containing protein (swd) inhibits bacteria invasion and dissemination in shrimp marsupenaeus japonicus. | the single whey acidic protein (wap) domain containing proteins (swds) in crustacean belong to type iii crustins and have antiprotease activities and/or antimicrobial activities. their functions in vivo in crustacean immunity need to be clarify. in this study, a new single wap domain containing protein (swd) was obtained from marsupenaeus japonicus, designated as mjswd. the full-length cdna of mjswd was 522 bp.the open reading frame of mjswd encoded a protein of 79 amino acids, with a 24 amino a ... | 2013 | 23665548 |
| plasmid- and chromosome-encoded siderophore anguibactin systems found in marine vibrios: biosynthesis, transport and evolution. | vibrio anguillarum is a marine pathogen that causes vibriosis, a hemorrhagic septicemia in aquatic invertebrate as well as vertebrate animals. the siderophore anguibactin system is one of the most important virulence factors of this bacterium. most of the anguibactin biosynthesis and transport genes are located in the 65-kb pjm1 virulence plasmid although some of them are found in the chromosome of this fish pathogen. over 30 years of research unveiled the role numerous chromosomal and pjm1 gene ... | 2013 | 23660776 |
| electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor. | an electrochemical disinfection system employing a honeycombed platinum coated titanium electrode was developed for the disinfection of seawater. cell suspensions (2 l, 10³ cells/ml) of the fish pathogens, vibrio alginolyticus, edwardsiella tarda, lactococcus garvieae and vibrio anguillarum were circulated in a reactor equipped with 10 sets of these electrodes at a flow rate of 200 ml/min with an applied potential of 1.0 v vs. ag/agcl reference electrode. the circulated cells were completely dis ... | 2013 | 23648105 |
| the response profiles of hspa12a and tctp from mytilus galloprovincialis to pathogen and cadmium challenge. | heat shock 70 kda protein 12a (hspa12a) is an atypical member of hsp70 family, and the translationally controlled tumor protein (tctp) is a novel hsp with chaperone-like activity. they are both involved in protecting organisms against various stressors. in the present study, the cdnas of hspa12a and tctp (called mghspa12a and mgtctp) were identified from mytilus galloprovincialis by race approaches. the full-length cdna of mghspa12a and mgtctp encoded a peptide of 491 and 171 amino acids, respec ... | 2013 | 23643947 |
| conserved structural complement component c3 in miiuy croaker miichthys miiuy and their involvement in pathogenic bacteria induced immunity. | complement component c3 is a key protein in the complement system whose activation is essential for all the important functions performed by this system. in this study, the complete c3 cdna sequence was isolated from the miiuy croaker (miichthys miiuy), which was high similarity to other complement c3. in this study, we report the primary sequence, the tissue expression profile, the polypeptide domain architecture and the phylogenetic analysis of miiuy croaker c3 gene. rapid amplification of the ... | 2013 | 23643875 |
| a novel beta-defensin antimicrobial peptide in atlantic cod with stimulatory effect on phagocytic activity. | a novel defensin antimicrobial peptide gene was identified in atlantic cod, gadus morhua. this three exon/two intron defensin gene codes for a peptide precursor consisting of two domains: a signal peptide of 26 amino acids and a mature peptide of 40 residues. the mature cod defensin has six conserved cysteine residues that form 1-5, 2-4 and 3-6 disulphide bridges. this pattern is typical of beta-defensins and this gene was therefore named cod beta-defensin (defb). the tertiary structure of defb ... | 2013 | 23638029 |
| identification of cathepsin b in the razor clam sinonovacula constricta and its role in innate immune responses. | cathepsin b, a lysosomal cysteine protease, has drawn much attention in vertebrates. however, very little is known about the functions of cathepsin b in bivalves. in this study, we identified the cathepsin b gene in the razor clam sinonovacula constricta. the protein has a typical cysteine protease structure, comprising a 15-residue putative signal peptide, a 75-residue propeptide and a 249-residue mature domain. in the mature domain, there is an occluding loop, an oxyanion hole (gln) and a cata ... | 2013 | 23632254 |
| chemical shift assignments and secondary structure prediction of the phosphorelay protein vanu from vibrio anguillarum. | vibrio anguillarum is a biofilm forming gram-negative bacterium that survives prolonged periods in seawater and causes vibriosis in marine life. a quorum-sensing signal transduction pathway initiates biofilm formation in response to environmental stresses. the phosphotransferase protein vanu is the focal point of the quorum-sensing pathway and facilitates the regulation between independent phosphorelay systems that activate or repress biofilm formation. here we report the (1)h, (13)c, and (15)n ... | 2014 | 23604692 |