Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| time-kill studies investigating the killing activity of caspofungin against candida dubliniensis: comparing rpmi-1640 and antibiotic medium 3. | we evaluated the in vitro activity of caspofungin against candida dubliniensis strains using mic and minimum fungicidal concentration (mfc) measurements and time-kill methodology. | 2008 | 18390882 |
| identification and differentiation of candida species from pediatric patients by random amplified polymorphic dna. | thirty-four candida isolates were analyzed by random amplified polymorphic dna using the primer opg-10:24 candida albicans; 4 candida tropicalis; 2 candida parapsilosis; 2 candida dubliniensis; 1 candida glabrata and 1 candida krusei. the upgma-pearson correlation coefficient was used to calculate the genetic distance between the different candida groupings. samples were classified as identical (correlation of 100%); highly related samples (90%); moderately related samples (80%) and unrelated sa ... | 2008 | 18368262 |
| comparative evaluation of atb fungus 2 and sensititre yeastone panels for testing in vitro candida antifungal susceptibility. | atb fungus 2 and sensititreyeastone are commercial methods for antifungal susceptibility testing of yeasts. the agreement between these two methods was assessed with a total of 133 candida strains (60 candida albicans, 18 candida dubliniensis, 29 candida glabrata, and 26 candida krusei). mic endpoints were established after 24 h of incubation at 36-/+1 degrees c by each method. intra-laboratory reproducibility of both methods was excellent (=or>99%). overall agreement between atb fungus 2 and se ... | 2008 | 18338919 |
| a prospective clinical trial of a real-time polymerase chain reaction assay for the diagnosis of candidemia in nonneutropenic, critically ill adults. | invasive candida infection among nonneutropenic, critically ill adults is a clinical problem that has received increasing attention in recent years. poor performance of extant diagnostic modalities has promoted risk-based, preemptive prescribing in view of the poor outcomes associated with inadequate or delayed antifungal therapy; this risks unnecessary overtreatment. a rapid, reliable diagnostic test could have a substantial impact on therapeutic practice in this patient population. | 2008 | 18260751 |
| candida dubliniensis meningitis as delayed sequela of treated c. dubliniensis fungemia. | we present a case of candida dubliniensis meningitis that developed 2 months after apparently successful treatment of an episode of c. dubliniensis candidemia in a heart-lung transplant recipient in australia. this case highlights the importance of follow-up in patients with candidemia or disseminated infection, especially in immunosuppressed patients. | 2008 | 18258133 |
| isolation of candida dubliniensis in a french hospital mycology laboratory. | 2008 | 18202000 | |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| performance of commercial latex agglutination tests for the differentiation of candida dubliniensis and candida albicans in routine diagnostics. | candida dubliniensis is phenotypically similar to candida albicans and may therefore be underdiagnosed in the clinical microbiology laboratory. the performance of bichro-dubli latex agglutination test for rapid species identification of c. dubliniensis was prospectively evaluated on 111 vaginal and 118 respiratory isolates. these had presumptively been identified as c. albicans/c. dubliniensis by their green colonies on chromagar candida plates. bichro-dubli test identifed 2 (1.8%) vaginal and 6 ... | 2007 | 18092961 |
| candida bracarensis detected among isolates of candida glabrata by peptide nucleic acid fluorescence in situ hybridization: susceptibility data and documentation of presumed infection. | molecular taxonomic studies have revealed new candida species among phenotypically delineated species, the best example being candida dubliniensis. this study was designed to determine the occurrence of two new molecularly defined species, candida bracarensis and candida nivariensis, which are closely related to and identified as candida glabrata by phenotypic assays. a total of 137 recent clinical isolates of c. glabrata identified by phenotypic characteristics was tested with c. bracarensis an ... | 2008 | 18077641 |
| potent anti-microbial activity of traditional chinese medicine herbs against candida species. | anti-candidial activities of eight traditional chinese medicinal (tcm) herbs were evaluated against six different candida species. tcm preparations were screened for antifungal activity using a standard agar diffusion assay. following identification of potential candidate herbs, their minimum inhibitory concentration (mic) values were determined using the standardised nccls m-27a broth microdilution assay. among tcm herbs, rhizoma coptidis had potent antifungal activity against candida glabrata, ... | 2008 | 18076592 |
| [an update on candida dubliniensis]. | eleven years ago, irish authors, using molecular biology, demonstrated the existence of candida dubliniensis, a new species of candida close to candida albicans. initially isolated from aids patients with oral candidiasis, this species was detected, even in immunocompetent patients. recently, with new, easy to implement identification tests (latex, immunochromatography), numerous epidemiological studies were undertaken. in most studies, c. dubliniensis was most often identified in the oral cavit ... | 2008 | 18065177 |
| multilocus sequence typing reveals that the population structure of candida dubliniensis is significantly less divergent than that of candida albicans. | the pathogenic yeast candida dubliniensis is phylogenetically very closely related to candida albicans, and both species share many phenotypic and genetic characteristics. dna fingerprinting using the species-specific probe cd25 and sequence analysis of the internal transcribed spacer (its) region of the ribosomal gene cluster previously showed that c. dubliniensis is comprised of three major clades comprising four distinct its genotypes. multilocus sequence typing (mlst) has been shown to be ve ... | 2008 | 18057125 |
| efficacy of chromogenic candida agar for isolation and presumptive identification of pathogenic yeast species. | chromogenic candida agar is a novel differential culture medium that is claimed to facilitate isolation and identification of candida albicans, candida tropicalis and candida krusei. the performance of this medium was evaluated for presumptive identification of 521 yeast strains, representing 23 different species, for detection of specimens containing yeast mixtures, and for direct isolation of yeast from blood cultures. all yeasts grew well on the medium following a 48-h incubation period at 37 ... | 2008 | 17986267 |
| morphogenesis control in candida albicans and candida dubliniensis through signaling molecules produced by planktonic and biofilm cells. | morphogenesis control by chemical signaling molecules is beginning to be highlighted in candida biology. the present study focuses on morphogenic compounds produced in situ by candida albicans and candida dubliniensis during planktonic and biofilm growth that may at least partially substantiate the effect promoted by supernatants in morphogenesis. for both species, planktonic versus biofilm supernatants were analyzed by headspace-solid-phase microextraction and gas chromatography-mass spectromet ... | 2007 | 17981993 |
| [rapid identification of candida dubliniensis using the bichro-dubli test]. | because of its considerable epidemiological relevance, accurate identification of candida dubliniensis should be routinely performed in clinical microbiology laboratories. in an attempt to facilitate this task, the usefulness of the bichro-dubli test (fumouze diagnostics, levallois-perret, france) was assessed. | 2007 | 17953898 |
| oral candida colonization of human immunodeficiency virus infected subjects in turkey and its relation with viral load and cd4+ t-lymphocyte count. | the association of asymptomatic oral candida colonization with the markers of immunodeficiency and hiv is not clear yet. in addition, the prevalence of different candida species colonizing the oral cavity of hiv-infected patients in turkey remains unknown. to evaluate these subjects, oral rinse samples were taken from 64 hiv-positive individuals who have come to the department of microbiology and clinical microbiology, istanbul medical faculty for viral load and cd4(+) t-lymphocyte count measure ... | 2007 | 17944711 |
| differential regulation of the transcriptional repressor nrg1 accounts for altered host-cell interactions in candida albicans and candida dubliniensis. | candida dubliniensis is genetically closely related to candida albicans, but causes fewer infections in humans and exhibits reduced virulence and filamentation in animal models of infection. we investigated the role of the c. dubliniensis transcriptional repressor-encoding gene cdnrg1 in regulating this phenotype. deletion of both copies of cdnrg1 increased the formation of true hyphae by c. dubliniensis in response to serum, exogenous camp and co2. in addition, deletion of cdnrg1 greatly enhanc ... | 2007 | 17927699 |
| a novel method for differentiation of candida dubliniensis from other candida species. | 2007 | 17901663 | |
| cellular surface hydrophobicity as an additional phenotypic criterion applied to differentiate strains of candida albicans and candida dubliniensis. | we have evaluated the cellular surface hydrophobicity (csh) determination as an additional criterion to differentiate candida albicans from candida dubliniensis. our results show that c. albicans when grown at 37 degrees c in sabouraud is always hydrophilic, and c. dubliniensis presents high csh levels. | 2008 | 17900846 |
| enzymatic and hemolytic activities of candida dubliniensis strains. | candida dubliniensis is an opportunistic yeast that has been recovered from several body sites in many populations; it is most often recovered from the oral cavities of human immunodeficiency virus-infected patients. although extensive studies on epidemiology and phylogeny of c. dubliniensis have been performed, little is known about virulence factors such as exoenzymatic and hemolytic activities. in this study we compared proteinase, hyaluronidase, chondroitin sulphatase and hemolytic activitie ... | 2007 | 17823746 |
| decarboxylation of sorbic acid by spoilage yeasts is associated with the pad1 gene. | the spoilage yeast saccharomyces cerevisiae degraded the food preservative sorbic acid (2,4-hexadienoic acid) to a volatile hydrocarbon, identified by gas chromatography mass spectrometry as 1,3-pentadiene. the gene responsible was identified as pad1, previously associated with the decarboxylation of the aromatic carboxylic acids cinnamic acid, ferulic acid, and coumaric acid to styrene, 4-vinylguaiacol, and 4-vinylphenol, respectively. the loss of pad1 resulted in the simultaneous loss of decar ... | 2007 | 17766451 |
| the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species. | in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ... | 2007 | 17761478 |
| extensive chromosome rearrangements distinguish the karyotype of the hypovirulent species candida dubliniensis from the virulent candida albicans. | candida dubliniensis and candida albicans, the most common human fungal pathogen, have most of the same genes and high sequence similarity, but c. dubliniensis is less virulent. c. albicans causes both mucosal and hematogenously disseminated disease, c. dubliniensis mostly mucosal infections. pulse-field electrophoresis, genomic restriction enzyme digests, southern blotting, and the emerging sequence from the wellcome trust sanger institute were used to determine the karyotype of c. dubliniensis ... | 2008 | 17719250 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| the involvement of physico-chemical interactions in the adhesion of candida albicans and candida dubliniensis to epithelial cells. | candida albicans and candida dubliniensis are two pathogenic yeasts particularly hazardous to immunocompromised patients. adhesion of yeast cells to epithelium is considered one of the virulence factors and its study is of major importance. the main aim of this study was the comparison of the influence of physico-chemical properties on the adhesion of c. albicans and c. dubliniensis to epithelium. two strains of each candida species were used in the adhesion assays to hela cells. adhered cells w ... | 2007 | 17714359 |
| oxidative stress response involving induction of protective enzymes in candida dubliniensis. | candida dubliniensis is a yeast species closely related to candida albicans, but in contrast to c. albicans, limited information is available on the virulence factors of this important fungal pathogen. the objective of the present study was to determine if this species was able to evoke an adaptive response to oxidants. c. dubliniensis, treated with a low concentration of either h(2)o(2) or methyl viologen (a superoxide generating agent), mounts an adaptive response that results in increased sur ... | 2007 | 17710623 |
| cell and antibody mediated immunity induced by vaccination with novel candida dubliniensis mannan immunogenic conjugate. | antigen-specific humoral response, as well as the induction of cellular immunity generated by candida dubliniensis mannan-human serum albumin (hsa) conjugate, a novel proposed immunogenic structure for subcellular vaccine, were evaluated in rabbits. mannan-hsa conjugate-induced specific igg and iga increased significantly after boosters (igg: p<0.001 and iga: p<0.01). mannan-hsa conjugate up-regulation of cell-surface expression of b-lymphocyte and granulocyte activation antigens cd25 and cd11b ... | 2007 | 17673147 |
| candida dubliniensis in recurrent polymicrobial tricuspid endocarditis. | this report describes a successful operative case of tricuspid infective endocarditis in an iv drug user. despite cessation of iv drug use, there were further recurrences. six different microorganisms with multiple portals of entry were identified, including one episode of fungal endocarditis, to our knowledge, this is the first case of recurrent infective endocarditis involving candida dubliniensis in an hiv-negative patient. | 2007 | 17651106 |
| one year prospective survey of candida bloodstream infections in scotland. | a 12 month survey of candidaemia in scotland, uk, in which every scottish hospital laboratory submitted all blood isolates of yeasts for identification, strain typing and susceptibility testing, provided 300 isolates from 242 patients, generating incidence data of 4.8 cases per 100,000 population per year and 5.9 cases per 100,000 acute occupied bed days; 27.9 % of cases occurred in intensive care units. more than half the patients with candidaemia had an underlying disease involving the abdomen ... | 2007 | 17644714 |
| rapid differentiation between fluconazole-sensitive and -resistant species of candida directly from positive blood-culture bottles by real-time pcr. | in view of both the delay in obtaining identification by conventional methods following blood-culture positivity in patients with candidaemia and the close relationship between species and fluconazole (flc) susceptibility, early speciation of positive blood cultures has the potential to influence therapeutic decisions. the aim was to develop a rapid test to differentiate flc-resistant from flc-sensitive candida species. three taqman-based real-time pcr assays were developed to identify up to six ... | 2007 | 17577063 |
| environmental source of candida dubliniensis. | we isolated candida dubliniensis from a nonhuman source, namely, tick samples from an irish seabird colony. the species was unambiguously identifi ed by phenotypic and genotypic means. analysis of the 5.8s rrna gene showed that the environmental isolates belong to c. dubliniensis genotype 1. | 2007 | 17553256 |
| [synthesis and antifungal activity of naturally occurring o- and c-prenylated flavanones]. | flavonoids is one of the most important groups of naturally occurring o-heterocycles possessing a wide range of biological activity. o- and c-prenylated flavanones as members of flavonoids also show remarkable biological activity such as antibacterial, antiviral, anti-tumor, antifungal, anti-hiv and enzyme inhibition activity. o- and c-prenylated flavanones possessing remarkable biological activity are representatives of this family of natural products. in this paper efficient synthetic methods ... | 2007 | 17518107 |
| evaluation of albicans id2 and biggy agar for the isolation and direct identification of vaginal yeast isolates. | in this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, albicans id(2) and biggy agar, as well as onto sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. these strains were identified as candida glabrata in 20 (31.8 %) samples, candida albicans in 15 samples (23.8 %), candida tropicalis in 10 samples (15.9 %), candida krusei in five samples (7.9 %), candida kefyr in five samples (7.9 %), cand ... | 2007 | 17510260 |
| candida dubliniensis isolated from the sputum of a patient with end-stage liver cirrhosis. | candida dubliniensis is rare and very similar to c. albicans. to date, detailed clinical reports on c. dubuliniensis recovered from an immunocompromised patient have not been described in japan. a 71-year-old man with end-stage liver cirrhosis had been treated for suppurative omarthritis due to methicillin-resistant staphylococcus aureus (mrsa). anti-mrsa agents and broad-spectrum antimicrobials but no antifungal agents had been administrated. c. dubliniensis, isolated from the sputum, was elimi ... | 2007 | 17473497 |
| differentiation of candida albicans from non-albicans yeast directly from blood cultures by gram stain morphology. | clusters of pseudohyphae are commonly seen on gram stain of blood cultures from patients with candida albicans fungemia. whether this morphologic feature is useful for differentiating c. albicans from other yeasts has not previously been systematically evaluated. yeast morphology on gram stain of blood cultures from consecutive patients with fungemia detected by the bactec automated blood culture system was prospectively assessed and correlated with the final culture-based yeast identification. ... | 2007 | 17447090 |
| assembly of the candida albicans genome into sixteen supercontigs aligned on the eight chromosomes. | the 10.9x genomic sequence of candida albicans, the most important human fungal pathogen, was published in 2004. assembly 19 consisted of 412 supercontigs, of which 266 were a haploid set, since this fungus is diploid and contains an extensive degree of heterozygosity but lacks a complete sexual cycle. however, sequences of specific chromosomes were not determined. | 2007 | 17419877 |
| adherence to hela cells, typing by killer toxins and susceptibility to antifungal agents of candida dubliniensis strains. | the aim of this study was to evaluate the adherence capability to hela cells, the susceptibility to killer toxins and the in vitro susceptibility to antifungal agents (etest method--ab biodisk, solna, sweden) of 9 candida dubliniensis isolates recovered from hiv+ and aids patients. the adherence test was strongly positive for strain atcc 777 and positive for all other strains. typing by killer toxins revealed two different biotypes among the 9 isolates studied: 888 and 688. only biotype 688 (atc ... | 2007 | 17384861 |
| rapid discrimination between candida albicans and candida dubliniensis by using real-time polymerase chain reaction. | several phenotypic methods have been used for the differentiation of candida albicans and candida dubliniensis, but molecular investigations are considered most reliable in their diagnostic value. here, we suggest a rapid real-time polymerase chain reaction assay where the discrimination was achieved through melting point analysis with the help of the nonspecific fluorescent dye sybrgreen. | 2007 | 17376634 |
| candida albicans and candida dubliniensis respond differently to echinocandin antifungal agents in vitro. | candida dubliniensis isolates tested for susceptibility to anidulafungin, caspofungin, and micafungin commonly showed artifactual regrowth and/or trailing effects with mic tests done under conditions involving a high initial yeast concentration. the artifacts were less common with candida albicans and seldom seen for either species under clinical and laboratory standards institute method m27-a test conditions. | 2007 | 17307974 |
| chlamydosporulation of candida albicans and candida dubliniensis on mustard agar. | chlamydospores are distinctive morphological forms characteristic of candida albicans, a phenomenon shared only with the closely related species candida dubliniensis. the production of chlamydospores has remained an important diagnostic criterion for the differentiation of c. albicans and c. dubliniensis from other yeast. we herein describe a new medium, mustard agar, for chlamydosporulation in candida. all the strains of c. dubliniensis and c. albicans tested produced chlamydospores on mustard ... | 2007 | 17302752 |
| prevalence and phenotypic evaluation of candida dubliniensis in pregnant women with vulvovaginal candidosis in a university hospital in ankara. | candida dubliniensis is very similar to candida albicans in terms of genotypic and phenotypic characteristics. as the hormonal milieu of the vagina during pregnancy, characterised by a lack of maternal cell-mediated immunity, enhances candida colonisation and serves as a risk factor for symptomatic expression, investigation into the isolation of c. dubliniensis in vaginal discharges of pregnant women with vulvovaginal candidosis was made. a total of 77 candida isolates obtained from 60 patients ... | 2007 | 17302742 |
| chlamydospore formation in candida albicans and candida dubliniensis--an enigmatic developmental programme. | chlamydospore formation has served for a long time for identification of the human fungal pathogen candida albicans, but the biological function of these structures still remains a secret. they have been proposed to allow survival in harsh environmental conditions, but this assumption remains to be proven. chlamydospores are produced only by the two closely related species c. albicans and candida dubliniensis, whose natural habitats are humans and warm-blooded animals, but not by other candida s ... | 2007 | 17302741 |
| [candida dubliniensis: a novel emerging species]. | over the past decade, the incidence of opportunistic fungal infections continues to increase. candida albicans remains the most important pathogenic yeast. since 1995, a novel candida specie has been identified and named candida dubliniensis. this specie shares many phenotypic characteristics with candida albicans, including the ability to produce germ tubes and chlamydospores. these similarities have caused significant problems in the identification of candida dubliniensis. a large variety of m ... | 2007 | 17264034 |
| effect of farnesol on candida dubliniensis morphogenesis. | cell-cell signalling in candida albicans is a known phenomenon and farnesol was identified as a quorum sensing molecule determining the yeast morphology. the aim of this work was to verify if farnesol had a similar effect on candida dubliniensis, highlighting the effect of farnesol on candida spp. morphogenesis. | 2007 | 17257261 |
| development of novel real-time pcr assays for detection and differentiation of eleven medically important aspergillus and candida species in clinical specimens. | in the present study, novel real-time pcr assays targeting the fungal its2 region were developed for the detection and differentiation of medically important aspergillus species (aspergillus fumigatus, aspergillus flavus, aspergillus nidulans, aspergillus niger, and aspergillus terreus) and candida species (candida albicans, candida dubliniensis, candida glabrata, candida krusei, candida parapsilosis, and candida tropicalis) using a lightcycler instrument. the combination of a group-specific and ... | 2007 | 17251398 |
| lower filamentation rates of candida dubliniensis contribute to its lower virulence in comparison with candida albicans. | candida albicans and c. dubliniensis are very closely related yeast species. in this study, we have conducted a thorough comparison of the ability of the two species to produce hyphae and their virulence in two infection models. under all induction conditions tested c. albicans consistently produced hyphae more efficiently than c. dubliniensis. in the oral reconstituted human epithelial model, c. dubliniensis isolates grew exclusively in the yeast form, while the c. albicans strains produced abu ... | 2007 | 17251042 |
| in vitro activities of voriconazole and five licensed antifungal agents against candida dubliniensis: comparison of clsi m27-a2, sensititre yeastone, disk diffusion, and etest methods. | we compared the in vitro activity of six antifungal agents against 62 isolates of candida dubliniensis by the clinical laboratory standards institute (clsi [formerly national committee for the clinical laboratory standards]) m27-a2, sensititre yeastone, disk diffusion, and etest methods and we studied the effect of the time of reading. for the azoles, voriconazole was the most potent in vitro followed by fluconazole, ketoconazole, and itraconazole. all the isolates were susceptible to amphoteric ... | 2006 | 17227209 |
| identification and characterization of mfa1, the gene encoding candida albicans a-factor pheromone. | in the opaque state, mtla and mtlalpha strains of candida albicans are able to mate, and this mating is directed by a pheromone-mediated signaling process. we have used comparisons of genome sequences to identify a c. albicans gene encoding a candidate a-specific mating factor. this gene is conserved in candida dubliniensis and is similar to a three-gene family in the related fungus candida parapsilosis but has extremely limited similarity to the saccharomyces cerevisiae mfa1 (scmfa1) and scmfa2 ... | 2007 | 17209123 |
| a fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis. | to date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. the increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. as a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. | 2006 | 17121679 |
| in vitro effects of micafungin against candida biofilms on polystyrene and central venous catheter sections. | long-term inserted and surgically implanted catheters can be colonised by candida spp. candida biofilms in vitro are often resistant to antifungal agents. the aim of this study was to investigate the in vitro activity of micafungin (mfg) against six candida spp. biofilms on polystyrene (ps) and central venous catheter (cvc) sections. safranin staining and differential interference contrast microscopy were used to demonstrate biofilm production. mfg activity was determined by the reduction in met ... | 2006 | 17101265 |
| bilateral chronic fungal dacryocystitis caused by candida dubliniensis in a neutropenic patient. | in recent years, candida species other than candida albicans have emerged as causes of human candidiasis, particularly in hiv-infected and other immunocompromised people. c. dubliniensis, a recently described species closely related to c. albicans, first isolated from patients with aids in dublin, has been implicated as an agent of oral candidiasis in hiv-positive people. however, it has also been recovered from hiv-negative people, with clinical signs of oral candidiasis and from the genital tr ... | 2006 | 17071804 |
| usefulness of candida id2 agar for the presumptive identification of candida dubliniensis. | chromagar candida and candida id2 are widely used for the isolation and presumptive identification of candida spp. based on the color of the colonies on these two media. we have studied the usefulness of these chromogenic media for differentiating candida dubliniensis from candida albicans isolates. one hundred isolates of c. dubliniensis and 100 c. albicans isolates were tested on candida id2, chromagar candida (chromagar), and chromagar candida reformulated by bbl. chromagar candida and chroma ... | 2006 | 17071554 |
| effect of farnesol on candida dubliniensis biofilm formation and fluconazole resistance. | candida dubliniensis and candida albicans are dimorphic fungal species with a number of pathogenic capabilities, including biofilm formation, systemic infection and development of fluconazole resistance. in this study, the ability of farnesol to disrupt these virulence capabilities was investigated. biofilm assessment and susceptibility studies indicated antifungal and antibiofilm properties for farnesol on both species with a disruptive effect on the cell membrane. synergy testing of farnesol a ... | 2006 | 17042756 |
| influence of acrylic resin polymerization methods and saliva on the adherence of four candida species. | there is limited information on the role of polymerization methods and saliva on the adherence of pathogenic candida species, with the exception of the adherence of candida albicans to acrylic resins and the relation of this to surface roughness and surface free energy, which appear to play a major role in the initial phases of microorganism adhesion. | 2006 | 16990072 |
| the effect of complete dentures with a metal palate on candida species growth in hiv-infected patients. | this in vivo investigation compared the oral candidal population between heat-cured acrylic resin and nickel-chromium-beryllium alloy in maxillary complete dentures in hiv-infected patients. | 2006 | 16958732 |
| evaluation of the new chromogenic medium candida id 2 for isolation and identification of candida albicans and other medically important candida species. | the usefulness of candida id 2 (caid2) reformulated medium (biomérieux, france) has been compared with that of the former candida id (caid; biomérieux), albicans id 2 (alb2; biomérieux), and chromagar candida (cac; chromagar, france) chromogenic media for the isolation and presumptive identification of clinically relevant yeasts. three hundred forty-five stock strains from culture collections, and 103 fresh isolates from different clinical specimens were evaluated. caid2 permitted differentiatio ... | 2006 | 16954270 |
| rapid identification of ascomycetous yeasts from clinical specimens by a molecular method based on flow cytometry and comparison with identifications from phenotypic assays. | this study was designed to compare the identification of ascomycetous yeasts recovered from clinical specimens by using phenotypic assays (pa) and a molecular flow cytometric (fc) method. large-subunit rrna domains 1 and 2 (d1/d2) gene sequence analysis was also performed and served as the reference for correct strain identification. a panel of 88 clinical isolates was tested that included representatives of nine commonly encountered species and six infrequently encountered species. the pa inclu ... | 2006 | 16954243 |
| esterase activity of candida species isolated from immunocompromised hosts. | a total of 149 clinical isolates of candida species isolated from immunocompromised patients were examined to ascertain their esterase activity by the tween 80 opacity test, which is a biochemical test used mainly to differentiate between candida albicans and candida dubliniensis. our results showed that c. albicans (92.3%), candida tropicalis (92.3%), candida parapsilosis (25%), c. dubliniensis (16.6%), candida inconspicua (100%), and candida lipolytica (100%) produced opacity halos through the ... | 2006 | 16854187 |
| detection of candida dubliniensis in patients with candidiasis in caracas, venezuela. | candida species are responsible for 80% of all nosocomial fungal infections. in 1995 a new yeast species was described, candida dubliniensis which shares with candida albicans characteristics. we have studied 109 yeast isolates identified as c. albicans to investigate the presence of c. dubliniensis by microbiological studies and pcr using dubr/dubf primers. positive results using microbiological tools were between 90 and 98%. two morphological and physiological of the 80 dna examined samples (2 ... | 2006 | 16854182 |
| comparison among tomato juice agar with other three media for differentiation of candida dubliniensis from candida albicans. | the purpose of the present study is to compare the tomato juice agar, a well known medium employed to observe ascospore formation, with niger seed agar, casein agar and sunflower seed agar, applied to a differentiation between c. dubliniensis and c. albicans. after 48 hours of incubation at 30 degrees c all 26 (100%) c. dubliniensis isolates tested produced chlamydospores on tomato juice agar as well as in the other three media evaluated. however, when we inoculated all media with c. albicans, t ... | 2006 | 16847498 |
| amplification of the hyphal wall protein 1 gene to distinguish candida albicans from candida dubliniensis. | the authors developed a new, simple, and reliable pcr/restriction fragment length polymorphism technique, using amplification of the hyphal wall protein 1 gene of candida albicans and its gene homologue in candida dubliniensis, to differentiate the two species of candida. performed with a new primer set, crr-f/crr-r, pcr produced two different fragments: one of 1,180 bp for c. albicans, and one of 930 bp for c. dubliniensis. | 2006 | 16825389 |
| molecular fingerprinting methods for the discrimination between c. albicans and c. dubliniensis. | opportunistic fungal pathogens are becoming increasingly important causes of both community-acquired and nosocomial infections. the most important fungal pathogens are yeast species belonging to the genus candida. these species show differences in levels of resistance to antifungal agents and mortality. consequently, it is important to correctly identify the causative organism to the species level. identification of candida dubliniensis in particular remains problematic because of the high degre ... | 2006 | 16700733 |
| candida albicans and candida dubliniensis: comparison of biofilm formation in terms of biomass and activity. | candida albicans and c. dubliniensis are two species responsible for oral candidiasis, especially in immunocompromised patients. microbial infection is preceded by adherence and biofilm formation. biofilm formation represents the most common form of c. albicans in the oral cavity and is considered to be one of the most important virulence factors. in this study, the biofilm formation ability of c. dubliniensis was compared with that of c. albicans in terms of biomass (quantified using crystal vi ... | 2006 | 16613134 |
| identification of candida dubliniensis in a diagnostic microbiology laboratory. | candida dubliniensis is an emerging yeast pathogen isolated mainly from immunocompromised patients. as molecular tests are currently unsuitable for use in routine diagnostic laboratories, we compared a variety of phenotypic techniques for differentiating c. albicans and c. dubliniensis. the tests included: colony colour on chromagar candida medium; growth at 37 degrees c and 45 degrees c; ability to produce germ tubes and chlamydospores; and the auxacolor system. the organisms included 105 isola ... | 2005 | 16602455 |
| molecular identification of candida dubliniensis isolated from oral lesions of hiv-positive and hiv-negative patients in são paulo, brazil. | candida dubliniensis is a new, recently described species of yeast. this emerging oral pathogen shares many phenotypic and biochemical characteristics with c. albicans, making it hard to differentiate between them, although they are genotypically distinct. in this study, pcr (polymerase chain reaction) was used to investigate the presence of c. dubliniensis in samples in a culture collection, which had been isolated from hiv-positive and hiv-negative patients with oral erythematous candidiasis. ... | 2006 | 16547575 |
| evaluation of 5 new media containing extracts of seeds applied to candida dubliniensis screening. | candida dubliniensis is a recently described pathogenic species that shares many phenotypic features with candida albicans and so may be misidentified in microbiologic laboratories. the aim of this study is to find a useful and cost-effective method suitable for screening c. dubliniensis before proceeding to further identification. we examined the colony morphology and chlamydospore production of 26 c. dubliniensis isolates and 100 c. albicans isolates on the following 5 proposed media: sesame s ... | 2006 | 16545931 |
| evaluation of bichro-dubli fumouze to distinguish candida dubliniensis from candida albicans. | we have evaluated the ability of the bichro-dubli fumouze (fumouze diagnostics, levallois-perret, france) latex agglutination test to identify colonies of candida dubliniensis grown on different media. the test was positive for 103 of 106 isolates of c. dubliniensis and negative for candida albicans and other candida species studied. the sensitivity and specificity of the test were 97.1% and 100%, respectively. the test is very rapid, simple, and reliable giving the same results independently of ... | 2006 | 16529902 |
| [utilization of tomato juice agar (v8 agar) in the presumptive identification of candida dubliniensis]. | we evaluated the capacity of tomato juice agar (v8 agar) to differentiate candida dubliniensis from candida albicans based on chlamydospore production. candida albicans (n= 93) and candida dubliniensis (n= 26) were studied; 100% of candida dubliniensis showed chlamydospores and in 92.5% of candida albicans isolates these elements were absent. these results suggest this medium as an alternative tool for presumptive differentiation between these species. | 2006 | 16501777 |
| rapid identification of fungal pathogens in bact/alert, bactec, and bbl mgit media using polymerase chain reaction and dna sequencing of the internal transcribed spacer regions. | we report a direct polymerase chain reaction/sequence (d-pcrs)-based method for the rapid identification of clinically significant fungi from 5 different types of commercial broth enrichment media inoculated with clinical specimens. media including bact/alert fa (biomérieux, marcy l'etoile, france) (n = 87), bactec plus aerobic/f (becton dickinson, microbiology systems, sparks, md) (n = 16), bactec peds plus/f (becton dickinson) (n = 15), bactec lytic/10 anaerobic/f (becton dickinson) (n = 11) b ... | 2006 | 16466900 |
| photosensitization of different candida species by low power laser light. | the aim of this study was to evaluate the effects of the laser radiation (685 nm) associated with photosensitizers on viability of different species of candida genus. suspensions of candida albicans, candida dubliniensis, candida krusei and candida tropicalis, containing 10(6) viable cells per milliliter were obtained with the aid of a neubauer's chamber. from each species, 10 samples of the cell suspension were irradiated with diode laser (685 nm) with 28 j/cm2 in the presence of methylene blue ... | 2006 | 16413196 |
| development and evaluation of a rapid latex agglutination test using a monoclonal antibody to identify candida dubliniensis colonies. | cell components of the dimorphic pathogenic fungus candida dubliniensis were used to prepare monoclonal antibodies (mabs). one mab, designated 12f7-f2, was shown by indirect immunofluorescence to be specific for a surface antigen of candida dubliniensis yeast cells. no reactivity was observed with other fungal genera or with other candida species, including candida albicans, that share many phenotypic features with c. dubliniensis. the use of different chemical and physical treatments for cell c ... | 2006 | 16390961 |
| candida dubliniensis bloodstream infection: a fatal case in a lung transplant recipient. | candida dubliniensis is an emerging opportunistic yeast initially identified as a cause of oropharyngeal candidiasis in human immunodeficiency virus-infected individuals, and recently associated with invasive disease in other immunocompromised hosts. certain diagnostic characteristics are shared with c. albicans, but differences in epidemiology, microbiology, and potentially clinical management are notable. we report a case of fatal c. dubliniensis bloodstream infection in a solid-organ transpla ... | 2005 | 16390404 |
| modification of adherence to plastic and to human buccal cells of candida albicans and candida dubliniensis by a subinhibitory concentration of itraconazole. | exposure to subinhibitory concentrations of antifungal agents can influence the adherence of candida spp. to the host cell. in this study the adherence of candida albicans atcc 10231 and candida dubliniensis cect 11455 to plastic and to human buccal epithelial cells was evaluated following pre-exposure to 0.5 x minimum inhibitory capacity (mic) of itraconazole and compared with the corresponding cellular surface hydrophobicity. the yeasts were grown in sabouraud broth or rpmi-1640 with itraconaz ... | 2006 | 16390344 |
| initial candida dubliniensis isolate in candida spp. positive haemocultures in turkey between 2001 and 2004. | candida dubliniensis which was first recognized in 1995 can be easily misidentified because of its phenotypic similarities with candida albicans. in this study blood samples of patients from various departments of ankara university medical faculty between january 2001-june 2004 were investigated for the distribution of candida spp. and the presence of c. dubliniensis. culture positive 67 fungi were included to the study. phenotypic tests such as chlamydospore formation, colony morphology on stai ... | 2006 | 16367821 |
| comparison of polar lipids from yeast and mycelial forms of candida albicans and candida dubliniensis. | the aim of the present study was to compare polar lipids of yeast and mycelial forms of both candida albicans and candida dubliniensis. cultures were harvested from lee's medium after incubation at 37 degrees c for 48 h. yeast and mycelial forms were washed, separated from one another, dried and lipids extracted and prepared for fast atom bombardment mass spectrometry analysis in the negative-ion mode. for fatty acids, differences between the yeast and mycelial forms were greater for c. dublinie ... | 2006 | 16367813 |
| influence of sub-inhibitory concentrations of antimicrobial agents on biofilm formation in indwelling medical devices. | biofilms of staphylococcus epidermidis and candida spp. are two of the most frequent factors of infections associated with the use of indwelling medical devices. several strategies have been proposed and/or developed to prevent infection. the aim of this study was to compare the effect of sub-inhibitory concentrations of anti-microbial agents on biofilm formation. biofilms of three strains of s. epidermidis and two of both candida albicans and candida dubliniensis were formed in the presence of ... | 2005 | 16353125 |
| heteroduplex mobility assay of the 26s rdna d1/d2 region for differentiation of clinically relevant candida species. | the heteroduplex mobility assay (hma) method using the pcr amplified d1/d2 region of the 26s rdna was tested for the differentiation of clinically relevant candida species. strains belonging to the same species are not expected to form heteroduplexes in this assay when their pcr products are mixed. d1/d2 hma experiments between all candida type strains tested showed heteroduplex formation, including candida albicans and candida dubliniensis. there was no heteroduplex formation when most clinical ... | 2006 | 16328861 |
| could candida dubliniensis be involved in lung fungus balls? | we describe a case of cavitary pneumonia due to candida dubliniensis along with fungemia due to candida kefyr in a leukemic patient. this is the first case of c. dubliniensis isolated in our laboratory. the identification was performed by phenotypic and molecular methods such as thermotolerance test, carbohydrate fermentation and polymerase chain reaction. | 2005 | 16309351 |
| vulvovaginal candidiasis in a flemish patient population. | increased resistance to fluconazole has been reported in oral, oesophageal and urinary candida isolates, but this has not been observed commonly in genital tract isolates. the rate of isolation of candida spp. and their susceptibility to amphotericin b, flucytosine and azoles were determined in a number of clinical practices in the city of ghent, belgium. patients with symptomatic vulvovaginal candidiasis (vvc) were treated with fluconazole, and the mycological and clinical outcomes were evaluat ... | 2005 | 16307555 |
| the effect of haart on salivary microbiota in the women's interagency hiv study (wihs). | study the prevalence of potentially pathogenic microorganisms in saliva of hiv-positive women in the women's interagency hiv study. | 2005 | 16301151 |
| supplementation of chromagar candida medium with pal's medium for rapid identification of candida dubliniensis. | chromagar candida medium is used for the isolation and identification of candida species, but it does not differentiate candida albicans from candida dubliniensis. this differentiation can be achieved by using pal's agar, which cannot be used in primary isolation. we have combined both media to obtain a new medium that can be used for the isolation and identification of c. dubliniensis in primary cultures. | 2005 | 16272515 |
| phenotypic methods and commercial systems for the discrimination between c. albicans and c. dubliniensis. | candida dubliniensis is a recently described candida species associated with oral candidosis that exhibits a high degree of phenotypic similarity to candida albicans. however, these species show differences in levels of resistance to antimycotic agents and ability to cause infections. therefore, accurate clinical identification of c. dubliniensis and c. albicans species is important in order to treat oral candidal infections. phenotypic identification methods are easy-to-use procedures for routi ... | 2005 | 16269032 |
| azole susceptibility and resistance in candida dubliniensis. | candida dubliniensis is a recently described species of pathogenic yeast that shares many phenotypic features with candida albicans. it is primarily associated with oral colonization and infection in hiv-infected individuals. isolates of c. dubliniensis are generally susceptible to commonly used azole antifungal agents; however, resistance has been observed in clinical isolates and can be induced by in vitro exposure. molecular mechanisms of azole resistance in c. dubliniensis include increased ... | 2005 | 16246083 |
| candida dubliniensis: ten years on. | candida dubliniensis was first described as a novel species in 1995. this organism is very closely related to the important human yeast pathogen, candida albicans. however, despite the very close phylogenetic relationship between c. albicans and c. dubliniensis and the fact that they share a large number of phenotypic traits, epidemiological and virulence model data indicate that the former is a far more successful pathogen. in order to investigate the molecular basis of the lower virulence of c ... | 2005 | 16213674 |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| detection of candida dubliniensis in venezuela. | over the past decades there has been a significant increase in fungal infections caused by candida species, and continues to be common in immunocompromised individuals infected with the human immunodeficiency virus (hiv). although candida albicans remains the fungal species most frequently isolated as an opportunistic oral pathogen, other non-albicans are often identified in this cohort of patients, including c. dubliniensis. this yeast is closely related to and shares many phenotypic characteri ... | 2005 | 16205971 |
| tobacco agar: a new medium for chlamydosporulation in candida albicans and candida dubliniensis. | chlamydospores are a distinctive morphologic feature of candida albicans and candida dubliniensis and aid in their identification. a new medium, tobacco agar, for chlamydosporulation in candida is described. all the strains of c. dubliniensis and 96% of isolates of c. albicans tested produced chlamydospores after 24 h incubation on tobacco agar, whereas none of the other seven species produced chlamydospores. | 2005 | 16178377 |
| high prevalence of oral colonization by candida dubliniensis in hiv-positive patients in argentina. | candida dubliniensis is a recently described yeast species, closely related to candida albicans. this work represents the first general survey of the carriage of c. dubliniensis in the oral cavities of hiv-positive patients in argentina. we studied 133 strains isolated from 162 hiv-positive patients, using the following identification tests: chlamydospore production on corn meal agar with tween 80; colony color on chromagar candida media; differential growth at 45 degrees c on potato dextrose ag ... | 2005 | 16178372 |
| a rapid screening test to distinguish between candida albicans and candida dubliniensis using nmr spectroscopy. | nuclear magnetic resonance (nmr) spectroscopy combined with a statistical classification strategy (scs) successfully distinguished between candida albicans and candida dubliniensis. 96% of the isolates from an independent test set were identified correctly. this proves that this rapid approach is a valuable method for the identification and chemotaxonomic characterisation of closely related taxa. most discriminatory regions were correlated with metabolite profiles, indicating biochemical differe ... | 2005 | 16165326 |
| comparison of visual 24-hour and spectrophotometric 48-hour mics to clsi reference microdilution mics of fluconazole, itraconazole, posaconazole, and voriconazole for candida spp.: a collaborative study. | a multicenter (six-center) study evaluated the performance (interlaboratory reproducibility, compatibility with reference methods, and categorical agreement) of 24-h visual and 48-h spectrophotometric mics. mics of fluconazole, itraconazole, voriconazole, and posaconazole were compared to reference 48-h microdilution broth visual mics (clsi [formerly nccls] m27-a2 document) for 71 isolates of candida spp. that included 10 fluconazole-resistant strains. twenty readings (5%) were reported as showi ... | 2005 | 16145103 |
| prevalence of candida dubliniensis fungemia at a large teaching hospital. | six cases of candida dubliniensis fungemia were identified during an 8-month period in hospitalized patients with various conditions, including human immunodeficiency virus infection. peptide nucleic acid fluorescent in situ hybridization analysis was used as a rapid and reliable test for differentiating c. dubliniensis from candida albicans, making it feasible to determine the prevalence of c. dubliniensis fungemia. | 2005 | 16142677 |
| [molecular identification of candida albicans and candida dubliniensis strains isolated from clinical samples]. | candida dubliniensis is a recently identified opportunistic pathogen, which has close phylogenetic relation with candida albicans. the aim of this study was the genotypic differentiation of 55 germ tube-positive candida strains isolated from clinical specimens (30 blood, 25 throat swab specimens). the isolates were phenotypically identified by api id 32c system, and genotypically identified by using polymerase chain reaction-restriction fragment lenght polymorphism (pcr-rflp) method. initially i ... | 2005 | 16128031 |
| differentiation of candida albicans and candida dubliniensis using a single-enzyme pcr-rflp method. | candida dubliniensis is a novel candida spp. that is similar to candida albicans with respect to several phenotypic characteristics. however, they differ from each other with respect to epidemiology, pathogenesis, and the rapid development of resistance to fluconazole. in the present study, we used a single-enzyme pcr-restriction fragment length polymorphism (rflp) technique to differentiate c. dubliniensis from c. albicans. the amplified its region of c. dubliniensis was digested once using the ... | 2005 | 16116258 |
| prevalence of candida dubliniensis among germ tube-positive candida isolates in a maternity hospital in kuwait. | in this study, 1644 germ tube-positive candida isolates from a maternity hospital was prospectively examined for the prevalence of candida dubliniensis. candida species were isolated from different clinical specimens, but majority (>90%) of them came from high vaginal swabs and urine specimens. the phenotypic and molecular identification methods for c. dubliniensis included production of rough colonies and chlamydospores on simplified sunflower seed agar, determination of assimilation profile by ... | 2005 | 16115107 |
| phenotypic and molecular characterization of candida dubliniensis isolates from clinical specimens in kuwait. | this study was carried out to characterize candida dubliniensis using phenotypic and molecular methods and to determine the occurrence of c. dubliniensis in clinical specimens in kuwait. | 2005 | 16103717 |
| sunflower seed husk agar: a new medium for the differentiation of candida dubliniensis from candida albicans. | a sunflower (helianthus annuus) seed husk agar medium has been developed and evaluated for differentiation of candida dubliniensis from candida albicans on the basis of colony morphology and chlamydospore production. all c. dubliniensis isolates (n=40) produced rough colonies with hyphal fringes and abundant chlamydospores whereas 101 of 105 (96.2%) c. albicans isolates produced smooth colonies with no evidence of chlamydospore production. since this medium is free from oil droplets, chlamydospo ... | 2005 | 16100426 |
| novel 5-flucytosine-resistant clade of candida dubliniensis from saudi arabia and egypt identified by cd25 fingerprinting. | dna fingerprinting of candida dubliniensis isolates using the species-specific probe cd25 previously showed that this species consists of two distinct groups, termed cd25 group i and cd25 group ii. the present study investigated the population structure of 30 c. dubliniensis oral isolates from saudi arabia and egypt using cd25 fingerprinting and rrna gene internal transcribed spacer region-based genotyping. cd25 fingerprinting analysis of these isolates revealed two distinct populations, the fir ... | 2005 | 16081946 |
| rapid identification of candida albicans and its related species candida stellatoidea and candida dubliniensis by a single pcr amplification using primers specific for the repetitive sequence (rps) of candida albicans. | candidiasis is caused by several candida species, of which candida stellatoidea and c. dubliniensis are phenotypically close to c. albicans. although current molecular biology-based techniques can distinguish between c. albicans and c. dubliniensis, a convenient tool that can distinguish c. stellatoidea from c. albicans has not yet been developed. | 2005 | 16054340 |
| incubation at room temperature may be an independent factor that induces chlamydospore production in candida dubliniensis. | production of chlamydospores is one of the phenotypic features used to differentiate candida albicans and candida dubliniensis. c. albicans produces few chlamydospores on only cornmeal/rice-tween agar at room temperature, whereas c. dubliniensis produces abundant chlamydospores at this temperature both on cornmeal agar and some other commonly used media. we tried to determine whether the room temperature is the main factor that induces chlamydospore production of c. dubliniensis, regardless of t ... | 2005 | 16054328 |
| first reported case of endocarditis caused by candida dubliniensis. | candida dubliniensis is an uncommon cause of bloodstream infection. we describe the first reported case of endocarditis caused by c. dubliniensis and the use of a rapid and novel real-time pcr assay based on the internal transcribed spacer 2 variable region of the rrna operon that was used to identify this organism. | 2005 | 15956451 |