Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| detection of four plasmodium species in blood from humans by 18s rrna gene subunit-based and species-specific real-time pcr assays. | there have been reports of increasing numbers of cases of malaria among migrants and travelers. although microscopic examination of blood smears remains the "gold standard" in diagnosis, this method suffers from insufficient sensitivity and requires considerable expertise. to improve diagnosis, a multiplex real-time pcr was developed. one set of generic primers targeting a highly conserved region of the 18s rrna gene of the genus plasmodium was designed; the primer set was polymorphic enough int ... | 2004 | 15583293 |
| case report: plasmodium ovale infection acquired in malawi. | malaria due to plasmodium ovale is uncommon outside west africa. a 37-year-old male german who had returned from malawi four months previously presented in september 2003 because of fever recurring every two days. the patient had never been to west africa. microscopy of stained thick and thin blood films revealed p. ovale. this is the first report of a p. ovale infection acquired in malawi, east africa. malaria surveillance centres should monitor the possible emergence of autochtonous transmissi ... | 2004 | 15485706 |
| molecular analysis of plasmodium ovale variants. | complete dna sequences of the small subunit ribosomal rna (ssurrna) gene and partial sequences of three other loci were obtained from three variant-type and three classic-type plasmodium ovale isolates from southeast asia and compared with genbank-available data. three different ssurrna sequences (pov 1-3) were found in each variant-type isolate, and two different ssurrna sequences (poc 1-2) in each classic-type isolate. pov 1-3 were closer to sequences previously found in the cameroon and mal/m ... | 2004 | 15324543 |
| [characteristics of malaria cases diagnosed in edirne province between 1994-2002]. | in this study, the epidemiological characteristics of malaria cases in edirne province were investigated. between the years of 1994-2002, a total of 317,087 blood samples were collected from soldiers in the province with selective active surveillance and from the resident population with active or passive surveillance methods, by the medical staff of malaria control department and health centers, to search the presence of plasmodium. in 281 of them plasmodium spp. were detected, and the characte ... | 2004 | 15293910 |
| recombinant expression and enzymatic subsite characterization of plasmepsin 4 from the four plasmodium species infecting man. | plasmepsin 4 from plasmodium falciparum and orthologs from plasmodium malariae, plasmodium ovale and plasmodium vivax have been expressed in recombinant form, and properties of the active site of each enzyme characterized by kinetic analysis. a panel of chromogenic peptide substrates systematically substituted at the p3, p2, p2' and p3' positions was used to estimate enzyme/ligand interactions in the corresponding enzyme subsites based upon kinetic data. the kinetic parameters kcat, km and kcat/ ... | 2004 | 15287591 |
| evaluation of a new plate hybridization assay for the laboratory diagnosis of imported malaria in italy. | a new molecular diagnostic method "malaria-ibridogen" (amplimedical s.p.a.--bioline division, turin, italy) based on a plate-hybridization assay for the simultaneous detection and identification of human malaria parasites was evaluated in this study. a target dna sequence of the plasmodial 18s ribosomal rna gene was amplified by polymerase chain reaction (pcr) and hybridized in microtiter wells with five biotinylated probes each specific for plasmodium falciparum, p. vivax, p. malariae, p. ovale ... | 2004 | 15164627 |
| compensatory evolution in the human malaria parasite plasmodium ovale. | the fixation of neutral compensatory mutations in a population depends on the effective population size of the species, which can fluctuate dramatically within a few generations, the mutation rate, and the selection intensity associated with the individual mutations. we observe compensatory mutations and intermediate states in populations of the malaria parasite plasmodium ovale. the appearance of compensatory mutations and intermediate states in p. ovale raises interesting questions about popul ... | 2004 | 15020451 |
| development of a real-time pcr assay for detection of plasmodium falciparum, plasmodium vivax, and plasmodium ovale for routine clinical diagnosis. | a taqman-based real-time pcr qualitative assay for the detection of three species of malaria parasites-plasmodium falciparum, p. ovale, and p. vivax-was devised and evaluated using 122 whole-blood samples from patients who had traveled to areas where malaria is endemic and who presented with malaria-like symptoms and fever. the assay was compared to conventional microscopy and to an established nested-pcr assay. the specificity of the new assay was confirmed by sequencing the pcr products from a ... | 2004 | 15004078 |
| malaria relapse and recrudescence among travellers to the tropics. | this study describes 14 cases of relapse and recrudescence of malaria, treated between 1991 and 2003. in that period, 146 patients were hospitalized in the clinic of the institute in gdynia: 20 women and 126 men. in 103 cases the disease was caused by plasmodium falciparum, in 31 cases by plasmodium vivax, in 5 cases by plasmodium malariae, and in 2 cases by plasmodium ovale. five patients were found to have mixed infections, with either p. falciparum and p. vivax or p. falciparum and p. ovale. ... | 2003 | 14974782 |
| a systematic overview of published antimalarial drug trials. | systematic database searches identified 435 antimalarial drug treatment trials, involving 82,616 patients, conducted and published between 1966 and december 2002. of these trials 72% were randomised; 64 (15%) trials involved severe malaria, 47 (11%) studied plasmodium vivax, 3 plasmodium malariae or plasmodium ovale, and the remainder (74%) assessed treatment responses in uncomplicated falciparum malaria. twelve trials (2.7%) specifically evaluated antimalarial treatments in pregnant women. over ... | 2004 | 14964805 |
| [fourth specie of the malarial parasite in man (plasmodium ovale) and its discovery in the ussr]. | 1951 | 14837465 | |
| evaluation of the realart malaria lc real-time pcr assay for malaria diagnosis. | pcr-based methods have advantages over traditional microscopic methods for the diagnosis of malaria, especially in cases of low parasitemia and mixed infections. however, current pcr-based assays are often labor-intensive and not readily quantifiable and have the potential for contamination due to a requirement for postamplification sample handling. real-time pcr can address these limitations. this study evaluated the performance characteristics of a commercial malaria real-time pcr assay (reala ... | 2004 | 14766829 |
| external quality assessment schemes raise standards: evidence from the ukneqas parasitology subschemes. | the burden of parasitic disease imported into the temperate zone is increasing, and in the tropics remains very high. thus, high quality diagnostic parasitology services are needed, but to implement clinical governance a measure of quality of service is required. | 2003 | 14645352 |
| procalcitonin serum levels in tertian malaria. | procalcitonin (pct) is closely correlated with parasite burden and clinical outcome in falciparum malaria. the role of pct in tertian malaria has not previously been investigated. | 2003 | 14613513 |
| plasmepsin 4, the food vacuole aspartic proteinase found in all plasmodium spp. infecting man. | plasmepsins are aspartic proteinases of the malaria parasite, and seven groups of plasmepsins have been identified by comparing genomic sequence data available for the genes encoding these enzymes from plasmodium falciparum, plasmodium vivax, plasmodium knowlesi, plasmodium berghei, and plasmodium yoelii. the food vacuole plasmepsins typified by plasmepsin 4 from p. falciparum (pfpm4) constitute one of these groups. genes encoding the ortholog of pfpm4 have been cloned from plasmodium ovale, pla ... | 2003 | 14550891 |
| problems in the therapy for imported malaria in the united states. | errors in the diagnosis of imported malaria are increasingly recognized. however, there are few data on the treatment of malaria in the united states. | 2003 | 14504115 |
| a comparison of west african and west pacific strains of plasmodium ovale. | 1955 | 14373853 | |
| the pre-erythrocytic stage of plasmodium ovale. | 1955 | 14373852 | |
| [detection of plasmodium ovale in moscow among subjects infected in west africa]. | 1965 | 14345035 | |
| latent infections with plasmodium ovale malaria. | two cases of plasmodium ovale malaria acquired in west africa appeared as primary delayed attacks after one year's continuous residence in canada. both patients took full prophylactic doses of chloroquine before, during, and for several weeks after exposure. the inadequacy of the 4-aminoquinolines for protection against latent benign tertian malaria is noted, and the use of primaquine is recommended. paroxysms occurred in the evening and were accompanied by severe muscle pain, features considere ... | 1965 | 14296004 |
| [plasmodium ovale in french language african territories]. | 1963 | 14058232 | |
| studies on malaria in chimpanzees. x. the presumed second generation of the tissue phase of plasmodium ovale. | 1963 | 14015114 | |
| localization of alkaline phosphatase in the erythrocytic forms of plasmodium ovale. | 1962 | 13973314 | |
| unusual length of a plasmodium ovale infection. | 1958 | 13608056 | |
| [not available]. | 1958 | 13561026 | |
| plasmodium ovale in liberia. | 1957 | 13487966 | |
| studies on malaria in chimpanzees. iv. plasmodium ovale. | 1957 | 13458668 | |
| congenital malaria in england; plasmodium ovale. | 1957 | 13383202 | |
| [study on plasmodium ovale (stephens 1922) in french africa; relative incidence in cameroun]. | 1955 | 13329745 | |
| [natural infection caused by plasmodium ovale]. | 1955 | 13302997 | |
| [not available]. | 1955 | 13250373 | |
| observations on the morphology of a strain of plasmodium ovale. | 1952 | 13008360 | |
| [triple malaria infection in twin sisters from the democratic republic of congo]. | infections with 3 species of malaria parasites are rarely encountered and observed in less than 0.05% of cases. we came across such an infection in four year-old, monozygote twin sisters, coming from kinshasa (democratic republic of congo). in both of them, parasitemia was low or very low for p. falciparum and p. ovale and of 0.1-0.2% for p. malariae. the twin sisters presented with an iron deficiency anaemia, associated with an heterozygous sickle-cell anaemia and a moderate splenomegaly. the b ... | 2003 | 12836524 |
| use of a rapid, single-round, multiplex pcr to detect malarial parasites and identify the species present. | a new, rapid assay, based on a single-round, multiplex pcr, can be used to detect plasmodium falciparum, p. vivax, p. malariae or p. ovale in human blood. the pcr, which targets the conserved 18s small-subunit rna genes of the parasites, not only permits a malarial infection to be detected but also allows each plasmodium species present to be identified, even in cases of mixed infection. | 2003 | 12803868 |
| cross-species regulation of plasmodium parasitemia in semi-immune children from papua new guinea. | malariologists have long been fascinated by the question of whether plasmodium spp. interact in the human host. the first genetic study of the longitudinal dynamics of multiple plasmodium spp. and genotypes in humans has been completed in papua new guinea, where all four plasmodium spp. that infect humans are present. the broad implications of the data from this study are covered here and they show that the total parasite density of plasmodium species oscillates around a threshold and that peaks ... | 2003 | 12798085 |
| avoiding misdiagnosis of imported malaria: screening of emergency department samples with thrombocytopenia detects clinically unsuspected cases. | misdiagnosis of imported malaria is not uncommon and even abnormal routine laboratory tests may not trigger malaria smears. however, blind screening of all thrombocytopenic samples might be a possible way to detect clinically unsuspected malaria cases in the accident and emergency department (aed). | 2003 | 12757689 |
| automated detection of malaria-associated intraleucocytic haemozoin by cell-dyn cd4000 depolarization analysis. | laboratory tests for malaria are only performed if there is clinical suspicion of the disease, and a missed diagnosis contributes substantially to morbidity and mortality. malaria parasites produce haemozoin, which is able to depolarize light and this allows the automated detection of malaria during routine complete blood count analysis (cbc) with some abbott cell-dyn instruments. in this study, we evaluated the cell-dyn cd4000 with 831 blood samples submitted for malaria investigations. samples ... | 2003 | 12641610 |
| transmission of mixed plasmodium species and plasmodium falciparum genotypes. | we studied malaria transmission by comparing parasite populations in humans and mosquito vectors at the household level. blood samples were collected from all inhabitants for microscopic detection of gametocytes and polymerase chain reaction analysis. the next morning, blood-fed resting mosquitoes were collected inside the bed nets used by the individuals surveyed the previous afternoon. after 8 days of maintenance, mosquitoes were dissected, and midguts and salivary glands were recovered for po ... | 2003 | 12641406 |
| plasmodium ovale malaria acquired in central spain. | we describe a case of locally acquired plasmodium ovale malaria in spain. the patient was a spanish woman who had never traveled out of spain and had no other risk factors for malaria. because patients with malaria may never have visited endemic areas, occasional transmission of malaria to european hosts is a diagnostic and clinical challenge. | 2002 | 12498674 |
| rapid immunochromatographic malarial antigen detection unreliable for detecting plasmodium malariae and plasmodium ovale. | in order to determine the reliability of two commercial tests for the rapid detection of plasmodial antigen in cases of infection with plasmodium ovale and plasmodium malariae, the products were evaluated in four centers and a search of the relevant literature was performed. the results of the present and previous studies were compared. with overall sensitivities ranging between 18.8% and 47.6% for plasmodium malariae and between 20% and 31.3% for plasmodium ovale, it is evident that neither tes ... | 2002 | 12461593 |
| detection of imported malaria with the cell-dyn 4000 hematology analyzer. | the sensitivity and specificity of the cell-dyn 4000 hematology analyzer in the diagnosis of imported malaria were studied with samples from patients in an academic hospital setting. the performance of the cell-dyn 4000 hematology analyzer was compared with that of conventional diagnostic methods for malaria. the cell-dyn 4000 hematology analyzer detected hemozoin-containing depolarizing monocytes in 29 of 58 patients with malaria and 2 of 55 patients without malaria. the presence or absence of ... | 2002 | 12454179 |
| the assessment of antimalarial drug efficacy. | antimalarial drug efficacy in uncomplicated malaria should be assessed parasitologically in large, community-based trials, enrolling the age groups most affected by clinical disease. for rapidly eliminated drugs, a 28-day follow-up is needed, but, for slowly eliminated drugs, up to nine weeks could be required to document all recrudescences, and, when possible, the drug levels should also be measured. the who 14-day assessments are neither sensitive nor specific. in tropical plasmodium vivax and ... | 2002 | 12377597 |
| [current data on malaria in metropolitan france]. | epidemiological data from the french national reference center for imported diseases showed that the estimated number of cases of imported malaria in france increased from 5,940 in 1998 to 7,127 in 1999 and 8,056 in 2000. this three-year progression ended in 2001 when the number of estimated cases fell back to 7,223. it was due mainly to the concomitant increase in the number of people traveling to endemic zones especially in africa. in 2000 the median age of patients with imported malaria in fr ... | 2002 | 12244913 |
| [two cases of mixed infection of malaria diagnosed by pcr method]. | we here reported two japanese cases of mixed infections of plasmodium species, whose dnas were detected using the pcr test. one case was a 31 year-old male, who presented fever and fatigue, and had a travel history to kenya, cameroon and indonesia. smear test of his peripheral blood found the presence of plasmodium vivax, while nested-pcr diagnosis detected the dnas both p. vivax and plasmodium malariae. the other was a 54 year-old female suffering from general fatigue. she had been treated with ... | 2002 | 12212325 |
| a retrospective examination of sporozoite-induced and trophozoite-induced infections with plasmodium ovale: development of parasitologic and clinical immunity during primary infection. | a retrospective analysis was made of clinical and parasitologic parameters in patients with induced plasmodium ovale infection to document the initial clinical and parasitologic response and their subsequent development of clinical and parasitologic immunity, and to determine the effect of previous homologous and heterologous malaria on subsequent infection with this parasite. the prepatent periods were relatively uniform. eight patients injected with sporozoites that had been stored frozen had ... | 2002 | 12201582 |
| diagnostic criteria and risk factors for plasmodium ovale malaria. | plasmodium ovale is a common malaria parasite in africa, but the epidemiology of p. ovale malaria is poorly known. exposure to malaria, parasitemia, and morbidity were monitored for 6 years among the residents of a village in senegal. the relationship between the level of p. ovale parasitemia and fever risk were analyzed, and diagnostic criteria for clinical p. ovale malaria were established. then the relationships between the occurrence of p. ovale clinical malaria and a series of entomological ... | 2002 | 12195357 |
| two types of plasmodium ovale defined by ssu rrna have distinct sequences for ookinete surface proteins. | 2002 | 12106877 | |
| effect of primaquine standard dose (15 mg/day for 14 days) in the treatment of vivax malaria patients in thailand. | primaquine (8-aminoquinoline), the only effective drug to prevent relapses of the persistent liver forms of plasmodium vivax and plasmodium ovale, can induce hemolytic anemia in patients with glucose-6-phosphate dehydrogenase (g6pd) deficiency. the severity varies considerably among affected individuals. three hundred and sixty-four plasmodium vivax cases (342 g6pd-normal and 22 g6pd-deficient) were given a 3-day course of chloroquine (total dose 1,500 mg) followed by primaquine 15 mg a day for ... | 2001 | 12041544 |
| [imported malaria in children in 1999. study of the armand-trousseau hospital in paris]. | eighty children were treated at the hospital armand-trousseau (paris, france) for a malaria attack from 1999-01-01 to 2000-02-01. | 2002 | 11998422 |
| wide distribution of plasmodium ovale in myanmar. | the presence of plasmodium ovale has never been previously reported in myanmar. using blood samples obtained in many villages across the country between 1996 and 2000, molecular diagnosis of plasmodium species was made with semi- or full-nested polymerase chain reaction (pcr) with species-specific primers, followed by agarose gel electrophoresis to detect amplification products. the presence of p. ovale was also confirmed with the another pcr-based diagnosis, the microtiterplate hybridization (m ... | 2002 | 11903985 |
| detection of plasmodium ovale by the ict malaria p.f/p.v. immunochromatographic test. | 2001 | 11700187 | |
| [malaria in georgian republic]. | due to the prevailing natural conditions, georgia is an endemic country for malaria. all types were widespread in the country, with the exception of plasmodium ovale malaria. a campaign of large-scale and comprehensive measures undertaken by the authorities lead to complete elimination of malaria as an indigenous disease in 1970. | 2001 | 11548306 |
| parasitic procrastination: late-presenting ovale malaria and schistosomiasis. | a 29-year-old woman with ovale malaria (most likely contracted, together with asymptomatic schistosomiasis, in east africa two years previously) had fever, nausea and confusion, jaundice, anaemia, thrombocytopenia, hyponatraemia and hypokalaemia. she was initially diagnosed with and treated for blood-smear-positive vivax malaria. because of the unusual clinical presentation, blood was analysed by a malaria species-specific nested polymerase chain reaction (pcr) assay which identified plasmodium ... | 2001 | 11548081 |
| presence of three distinct ookinete surface protein genes, pos25, pos28-1, and pos28-2, in plasmodium ovale. | 2001 | 11295191 | |
| imported malaria treated in melbourne, australia: epidemiology and clinical features in 246 patients. | imported malaria is increasing in nonendemic countries, including australia. the objective of this study was to describe the epidemiology and clinical features of travelers with imported malaria presenting to a specialist infectious diseases hospital. | 2001 | 11285166 |
| authors' reply to "plasmodium ovale infection". | 2000 | 11262998 | |
| malaria epidemiological situation in italy and evaluation of malaria incidence in italian travelers. | malaria was endemic throughout the country until it was eradicated nearly 50 years ago. since then, mainly imported malaria cases have been reported to the national health service, with an increasing trend. the aim of this study was to present a detailed analysis of the current epidemiological situation of malaria in italy, and to make a first attempt to calculate the incidence of malaria in italian international travelers. | 2001 | 11182603 |
| difficulties in the prevention, diagnosis, and treatment of imported malaria. | imported malaria is quite common in the united states. increasing antimalarial drug resistance and changes in travel patterns may have important implications for the prevention, clinical presentation, and management of this disease. | 2000 | 10979063 |
| plasmodium ovale species in papua new guinea--lest we forget. | the microscopical diagnosis of plasmodium ovale infection is reviewed and its similarity to plasmodium vivax emphasized. its presence in papua new guinea has been recognized for many years, from a time not long after stephens first described the species in 1922, but it is rarely reported. there is no doubt of its presence in papua new guinea, together with p. falciparum, p. vivax and p. malariae, but its exact prevalence and distribution has not been determined. | 1998 | 10934553 |
| plasmodium ovale infections detected by pcr assay in lao pdr. | plasmodium ovate infection was demonstrated in 5 out of 143 inhabitants in a village in lao pdr by blood microscopy and pcr assay. although the specimen confirmed to be positive for p. ovale by microscopical examination was only one, the target sequences in the 18s rrna genes of malaria parasite detected in all of the five cases were consisted with those of p. ovale by the pcr assay. this is the first report concerning the presence of so many cases with p. ovale infection in lao pdr. | 1999 | 10928349 |
| "plasmodium ovale infection in delhi" published in indian journal of pediatrics jan-feb 1999; vol. 66 (1): 143-145. | 1999 | 10798097 | |
| plasmodium ovale malaria in delhi. | a case of ovale malaria in a child from delhi is reported. urban malaria ecotypes caused by p. ovale has never been seen before. characteristic morphological features of the parasite in stained blood film confirmed its identification. | 1999 | 10798048 |
| a study of the urban malaria transmission problem in khartoum. | a study of malaria prevalence and transmission was carried out in khartoum, the capital of sudan. the sentinel sites were el manshia, an urban area on the blue nile and ed dekheinat, a lower-income peri-urban area bordering the white nile. anopheles arabiensis, the only malaria vector encountered, was present throughout the year although vector density varied seasonally. plasmodium falciparum was the only species found in el manshia. in ed dekheinat p. falciparum, plasmodium ovale and plasmodium ... | 2000 | 10708656 |
| [diagnostic test to identify human plasmodium species by the quantitative buffy coat test]. | the quantitative buffy coat system (qbc test) was designed for rapid diagnosis of malaria by identifying the presence of hemoparasites. the main drawback of the technique is failure to identify the plasmodium species. the purpose of this study was to attempt to remedy this problem by studying the distribution of the parasites at the bottom of the test tube. indeed since the qbc test is based on gradient centrifugation of blood components, the distribution of the parasites in the test tube depend ... | 1999 | 10701207 |
| [increase of malaria among migrants in amsterdam-zuidoost]. | in a general practice in amsterdam southeast in 1998 a delayed first attack of plasmodium ovale infection was diagnosed in a 13-year-old girl from ghana, malaria tropica with a low parasitaemia index in a 43-year-old ghanaian man and a 8-year-old ghanaian girl, and plasmodium vivax infection in a 44-year-old surinam woman. the ghanaian patients had visited their native country, the surinam woman had contracted the infection during a visit to india. all patients responded well to antimalaria medi ... | 2000 | 10674108 |
| erythrocyte fy antigen phenotyping helps differentiate so-called benign tertian malarias. | isolated cases of malaria are increasing in frequency in nonendemic countries. blood film examination remains a mainstay of diagnosis of these sporadic cases because immunologic and molecular methods are unavailable, expensive, and problematic. two tertian malarial species, plasmodium vivax and plasmodium ovale, may appear to be similar morphologically. plasmodium ovale infection is infrequent, and misdiagnosis of this species is common. plasmodium vivax infection can be ruled out, however, if a ... | 2000 | 10656744 |
| cloning and characterization of iron-containing superoxide dismutase from the human malaria species plasmodium ovale, p. malariae and p. vivax. | the iron-containing superoxide dismutase (fesod) gene from three human malaria species, namely plasmodium ovale, p. malariae and p. vivax, was amplified by polymerase chain reaction, cloned and then sequenced. comparisons of their deduced amino acid sequences with that of the fesod from p. falciparum revealed a very low polymorphism at the fesod locus in human malaria species. one p. ovale and the p. vivax fesod genes presented the same nucleotide sequence as that of the p. falciparum strain hb3 ... | 1999 | 10599926 |
| plasmodium ovale in southern africa. | 1999 | 10492808 | |
| usefulness of seminested multiplex pcr in surveillance of imported malaria in spain. | the use of a new pcr-based method for the diagnosis of malaria in the spanish malaria reference laboratory has promoted an increase in confirmed cases of malaria. from august 1997 to july 1998, a total of 192 whole-blood samples and 71 serum samples from 168 patients were received from the hospitals of the spanish national health system. most of the patients came from west-central african countries (85%). this molecular method showed more sensitivity and specificity than microscopy, detecting 12 ... | 1999 | 10488189 |
| how prevalent are plasmodium ovale and p. malariae in east asia? | plasmodium ovale and plasmodium malariae, two of the four human malaria parasites, are usually found at very low prevalence in east asia, even in areas with intense malaria transmission. in this article, fumihiko kawamoto, qing liu, marcelo ferreira and indah tantular review data obtained in recent field surveys, using alternative diagnostic methods such as acridine orange staining and pcr-based methods, to evaluate the prevalence of these two malaria species in east asia. they argue that these ... | 1999 | 10481157 |
| [rapid resolution of plasmodium ovale malarial attacks using artesunate (arsumax)]. | this randomized, non-comparative clinical trial without placebo was carried out to assess the efficacy and tolerance of artesunate for treatment of acute plasmodium ovale malarial attacks. thirty cameroonese patients were included. all presented acute plasmodium ovale malarial attacks with parasitemia in excess of 500 asexual forms per mm3. four days after treatment with artesunate, all 30 patients were asymptomatic with no parasitemia. reduction rates were 93.9 p. 100 for asexual forms and 75.4 ... | 1999 | 10472581 |
| imported tertian malaria resistant to primaquine. | in plasmodium vivax and plasmodium ovale malaria, some of the liver stage parasites remain dormant. the activation of these dormant forms (called hypnozoite) can give rise to relapse weeks, months or years after the initial infection. to prevent relapses, a course of primaquine may be given as terminal prophylaxis to patients. different strains of plasmodium vivax vary in their sensitivity to primaquine and, recently, cases of relapse of plasmodium vivax after this standard primaquine therapy we ... | 1999 | 10461431 |
| rare quadruple malaria infection in irian jaya indonesia. | we report an exceptional finding from a blood slide collected in a remote area in the western half of new guinea island (irian jaya province, indonesia). one adolescent patient was found patently coinfected with all 4 known human malaria species, plasmodium falciparum, plasmodium vivax, plasmodium malariae, and plasmodium ovale. diagnostic erythrocytic stages of all 4 species were clearly seen in the peripheral blood. a nested polymerase chain reaction, using species-specific primer pairs to det ... | 1999 | 10386460 |
| malaria prevention in travelers. | the prevention of malaria in travelers is becoming a more challenging clinical and public health problem because of the global development of drug-resistant plasmodium strains of malaria and the increasing popularity of travel to exotic locales. travelers can reduce their risk of acquiring malaria by using bed netting, wearing proper clothing and applying an insect repellent that contains n,n-diethyl-meta-toluamide. chloroquine, once the standard agent for weekly malaria prophylaxis, is no longe ... | 1999 | 10323359 |
| multispecies plasmodium infections of humans. | we analyzed point-prevalence data from 19 recent studies of human populations in which either plasmodium ovale or plasmodium vivax co-occur with plasmodium falciparum and plasmodium malariae. although the only statistical interactions among, sympatric congeners are pairwise, the frequencies of mixed-species infections relative to standard hypotheses of species sampling independence show no strong relation to overall malaria prevalence. the striking difference between the p. falciparum-p. malaria ... | 1999 | 10207356 |
| retrospective study of malaria cases treated in newcastle general hospital between 1990 and 1996. | malaria, in particular falciparum malaria, continues to pose a substantial risk to travelers to endemic areas. | 1998 | 9876194 |
| plasmodium ovale in south africa. | 1998 | 9861386 | |
| plasmodium ovale in a highly malaria endemic area of senegal. | during 4 months, from june to september 1990, the population of dielmo village, senegal, an area of intense and perennial malaria transmission, was enrolled in a follow-up study including daily clinical surveillance and bi-weekly malaria parasitaemia monitoring. thick blood film examinations indicated that 48.5% of children (49/101) and 32.4% of adults (34/105) were infected at least once by plasmodium ovale during the study period; 148 distinct episodes of patent parasitaemia were observed, wit ... | 1998 | 9861368 |
| current status and prospects of malaria vaccines. | malaria is a parasitic disease caused by four plasmodium species infecting humans, plasmodium falciparum, plasmodium vivax, plasmodium ovale, and plasmodium malariae. the parasite is transmitted by the anopheles mosquitoes that flourish in warm climates. these vectors thus are present in many tourist areas of the world, including africa, asia, and south america. known in china, as far back as 2700 bc, malaria was controlled by medicinal plants or drugs. this was until drug resistance occurred, b ... | 1995 | 9815369 |
| non-immune patients in the democratic republic of são tomé e principe reveal a high level of transmission of p. ovale and p. vivax despite low frequency in immune patients. | malaria parasite species circulating in immune inhabitants of the democratic republic of são tomé e principe (drstp) and those acquired by non-immune travellers returning from this country have been compared. using sensitive detection and species identification by pcr, plasmodium parasites were found in the blood of 16 of the 43 travellers, who reported during the first 8 months of 1995 to a clinical diagnosis laboratory in lisbon. plasmodium vivax was found in four (25%) and plasmodium ovale in ... | 1998 | 9698266 |
| plasmodium ovale in lao pdr. | 1997 | 9656397 | |
| high prevalence of plasmodium malariae and plasmodium ovale in malaria patients along the thai-myanmar border, as revealed by acridine orange staining and pcr-based diagnoses. | the prevalence of the four human malaria parasites was investigated among malaria patients at northern, central and southern towns in thailand along the border with myanmar between september 1995 and may 1996. thin smears obtained from 548 thai and burmese patients were reviewed by an acridine orange staining method, and many mixed infections with two to four species, including p. malariae and p. ovale, were detected. these diagnostic results were compared with those by two pcr-based diagnoses, ... | 1998 | 9623932 |
| a variant of plasmodium ovale; analysis of its 18s ribosomal rna gene sequence. | we report here a new variant of human malaria parasite found by comparison of diagnostic results obtained from a new dna diagnostic method named microtiter plate-hybridization (mph) and traditional microscopic method. total five cases of malaria were diagnosed as microscopy-positive but mph-negative; one case was found in epidemiological research in vietnam and four cases were obtained from imported malaria in japan. although they were quite similar to typical p. ovale morphologically in microsc ... | 1997 | 9586115 |
| detection of plasmodium ovale malaria parasites by species-specific 18s rrna gene amplification. | a polymerase chain reaction (pcr) assay was developed for the specific detection of plasmodium ovale, one of the four malaria parasites that infect humans. on the basis of sequence variation of the plasmodium 18s ribosomal rna (rrna) gene, oligonucleotide primers for pcr were designed to amplify various fragments of the p. ovale gene. using a recombinant plasmid with the complete p. ovale 18s rrna gene as target, 59 primer combinations were tested so that at least one of the pairs was species-sp ... | 1997 | 9500806 |
| pyronaridine for treatment of plasmodium ovale and plasmodium malariae infections. | the clinical efficacy of oral pyronaridine was assessed in 22 symptomatic cameroonian patients infected with plasmodium ovale or plasmodium malariae. all patients were cured on or before day 4. in vitro drug assays confirmed the sensitivity of p. ovale and p. malariae isolates to chloroquine and pyronaridine. | 1997 | 9333075 |
| atovaquone plus proguanil is an effective treatment for plasmodium ovale and p. malariae malaria. | 1996 | 9015517 | |
| short report: rosette formation in plasmodium ovale infection. | red blood cells infected by mature stages of plasmodium ovale obtained from a 56-year-old thai patient formed rosettes readily with uninfected erythrocytes. ex vivo, the ring stage-infected erythrocytes matured well under the in vitro conditions used for p. falciparum culture, and the infected erythrocytes formed rosettes when the parasites became mature trophozoites. these rosettes were stable and remained intact until completion of schizogony. plasmodium ovale rosettes were similar to those fo ... | 1996 | 8940990 |
| sequence variation in the 18s rrna gene, a target for pcr-based malaria diagnosis, in plasmodium ovale from southern vietnam. | field surveys of malaria were performed in southern vietnam by using an acridine orange staining method for rapid diagnosis and a pcr-based, microtiter plate hybridization method for accurate diagnosis. a total of three patients of plasmodium ovale infection were detected, but pcr-amplified dna of the p. ovale isolates from two of the patients did not hybridize with the p. ovale-specific probe. analysis of the target sequence in the 18s rrna gene indicated that in the dna of isolates from both p ... | 1996 | 8862600 |
| dna diagnosis of malaria using microtiter plate-hybridization. | we have developed a new diagnostic method "microtiter plate-hybridization" (mph) for the detection of human malaria parasite in which the target dna sequence of the 18s ribosomal rna gene is amplified by polymerase chain reaction and hybridized with the species-specific probes immobilized on a microtiter well. the pcr products bound on a well are visualized by the biotin-streptavidin system and the following chromogenic reaction. this method has allowed us to detect and identify the four species ... | 1995 | 8841641 |
| the nigerian i/cdc strain of plasmodium ovale in chimpanzees. | the chimpanzee is the only animal host currently available that can support the development of the human malaria parasite plasmodium ovale. thirty-one infections with the nigerian i/cdc strain were induced in splenectomized chimpanzees. maximum parasite counts ranged from 1,240 to 127,224/microliters. infections were transient and unpredictable. anopheles stephensi, anopheles gambiae, anopheles freeborni, and anopheles dirus mosquitoes were infected by feeding through parafilm membranes on hepar ... | 1996 | 8636850 |
| [malaria and drug resistance]. | drug resistance is a major problem in malaria. the resistance mechanism remains unresolved but contributing factors are probably heavy drug use, parasite selection, cross resistance and genetic influences of drugs. plasmodium ovale en p. malariae are sensitive to the current antimalarial drugs. p. vivax has some chloroquine resistant strains, notably on papua new guinea, irian jaya and other islands in the pacific. the geographical distribution of p. falciparum strains resistant to proguanil and ... | 1996 | 8618636 |
| post-transfusion acquired malaria complicating orthotopic liver transplantation. | early infectious complications within the first 3 months of orthotopic liver transplantation are common and are associated with significant morbidity and mortality. here we report the first case of transfusion-acquired malaria in an orthotopic liver transplantation recipient. the patient was found to have plasmodium ovale malaria during evaluation of a severe febrile illness. the infection was traced to a platelet transfusion and responded to treatment with chloroquine. risk factors associated w ... | 1996 | 8607511 |
| plasmodium ovale: observations on the parasite development in saimiri monkey hepatocytes in vivo and in vitro in contrast with its inability to induce parasitemia. | exoerythrocytic stage parasites of the human malaria parasite plasmodium ovale were cultured in vitro by inoculating primary cultures of hepatocytes from saimiri sciureus boliviensis monkeys with sporozoites. morphology and size of the liver stages were similar to previous in vivo descriptions in humans and chimpanzees. saimiri monkeys did not develop parasitemia after repeated inoculations with p. ovale sporozoites. however, liver-stage parasites were observed in liver biopsies performed 7 days ... | 1994 | 8206138 |
| in vitro cultivation of exoerythrocytic stages of the simian malaria parasites plasmodium fieldi and plasmodium simiovale in rhesus monkey hepatocytes. | exoerythrocytic stage parasites of plasmodium fieldi and plasmodium simiovale, 2 simian malaria parasites related to the human malaria parasite plasmodium ovale, were cultured in vitro by inoculating primary cultures of hepatocytes from rhesus monkeys (macaca mulatta) with sporozoites. less than 1% of sporozoites developed into schizonts for either species. structure and size of the liver stages in both species were similar to previous in vivo descriptions, and the time required for in vitro mat ... | 1994 | 8195940 |
| pharmacokinetics and kinetic-dynamic modeling of an 8-aminoquinoline candidate anticyanide and antimalarial drug (wr242511). | malaria is a major cause of health problems in a large portion of the world. the 8-aminoquinoline compound, primaquine, is one of the only compounds useful for relapses of plasmodium vivax and plasmodium ovale malaria. primaquine has several toxicities that include methemoglobinemia and hemolytic anemia. the induction of methemoglobinemia is a treatment for cyanide poisoning. we studied the pharmacokinetics and pharmacodynamics (percentage methemoglobin) for wr242511, an 8-aminoquinoline primaqu ... | 1994 | 8070311 |
| accuracy of routine laboratory diagnosis of malaria in the united kingdom. | to study the accuracy of routine laboratory diagnosis of malaria with the aim of improving accuracy in diagnosis in the future. | 1994 | 7962629 |
| inoculum size and severity of malaria induced with plasmodium ovale. | although it might be expected that the size of the infecting inoculum would influence the severity of the consequent malaria, evidence that it does so is scarce. using records from 80 non-immune neurosyphilis patients who received malaria therapy with a single strain of plasmodium ovale by blood inoculation, the relationships between the number of trophozoites inoculated, the prepatent period, and measures of severity of the resulting malaria were examined. the number of trophozoites was not rel ... | 1995 | 7785527 |
| [a case of plasmodium ovale malaria with thrombocytopenia and an abnormality grade in fdp concentration despite the use of chloroquine as a malaria prophylaxis]. | a 45-year-old japanese male, who had been to the central african republic, was admitted to our hospital because of high fever with chills on july 29, 1994. he used chloroquine as a malaria prophylaxis during his stay and for 6 weeks after his return to japan. on admission, plasmodium ovale was detected in his blood smears and in the dna analysis. he was treated successfully with chloroquine (1500 mg over 3 day period) and primaquine (15 mg/day for 14 days beginning day 4). disappearance of malar ... | 1995 | 7751755 |
| plasmodium: genus-conserved primers for species identification and quantitation. | stable rnas have regions of primary sequence that are nearly identical in every member of the plasmodium genus and not found in the host or in other common pathogens. several "genus-conserved" sequences, which flank hypervariable regions, were identified within the small subunit ribosomal rna of plasmodium species. primers based on these conserved sequences permit amplification of species- or possibly even strain-specific sequences from samples of unknown composition. as an example of this appro ... | 1995 | 7556560 |
| a case of imported plasmodium ovale infection in trinidad, west indies. | 1983 | 6868574 |