Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| comparisons of resistance of cf and non-cf pathogens to hydrogen peroxide and hypochlorous acid oxidants in vitro. | abstract: background: cystic fibrosis (cf) lung disease has a unique profile of pathogens predominated by pseudomonas aeruginosa (psa) and staphylococcus aureus (sa). these microorganisms must overcome host immune defense to colonize the cf lungs. polymorphonuclear neutrophils are a major component of the host defense against bacterial infection. a crucial microbicidal mechanism is the production of oxidants including hydrogen peroxide (h2o2) and hypochlorous acid (hocl) by neutrophils to achiev ... | 2011 | 21599970 |
| a colorimetric assay of 1-aminocyclopropane-1-carboxylate (acc) based on ninhydrin reaction for rapid screening of bacteria containing acc deaminase. | aims: acc (1-aminocyclopropane-1-carboxylate) deaminase activity is an efficient marker for bacteria to promote plant growth by lowering ethylene levels in plants. we aim to develop a method for rapidly screening bacteria containing acc deaminase, based on a colorimetric ninhydrin assay of acc. methods and results: a reliable colorimetric ninhydrin assay was developed to quantify acc using heat-resistant polypropylene chimney-top 96-well pcr plates, having the wells evenly heated in boiling wa ... | 2011 | 21599721 |
| endophytes and their potential to deal with co-contamination of organic contaminants (toluene) and toxic metals (nickel) during phytoremediation. | the aim was to investigate if engineered endophytes that are capable of degrading organic contaminants, and deal with or ideally improve uptake and translocation of toxic metals, can improve phytoremediation of mixed organic-metal pollution. as a model system, yellow lupine was inoculated with the endophyte burkholderia cepacia vm1468 possessing (a) the ptom-bu61 plasmid, coding for constitutive toluene/tce degradation, and (b) the chromosomally inserted ncc-nre ni resistance/sequestration syste ... | 2011 | 21598790 |
| in vitro activity of bal30072 against burkholderia pseudomallei. | burkholderia pseudomallei is an intrinsically antibiotic-resistant category b priority pathogen and the aetiological agent of melioidosis. treatment of b. pseudomallei infection is biphasic and lengthy in order to combat the acute and chronic phases of the disease. acute-phase treatment preferably involves an intravenous cephalosporin (ceftazidime) or a carbapenem (imipenem or meropenem). in this study, the anti-b. pseudomallei efficacy of a new monosulfactam, bal30072, was tested against labora ... | 2011 | 21596528 |
| isolation and characterization of a 4-nitrotoluene-oxidizing enzyme from activated sludge by a metagenomic approach. | to isolate a biocatalytic enzyme, metagenomic libraries were constructed in fosmids from samples of activated sludge used to treat coke plant wastewater. six indigo-producing clones were isolated from approximately 40,000 metagenomic clones in the search for the oxygenase responsible. in vitro mutagenesis and whole-sequencing revealed one open reading frame to be responsible for the production of indigo in the fosmid clones. the deduced sequence of the gene product showed 60% identity with 2-nap ... | 2010 | 21576864 |
| pseudomonas quinolone signal affects membrane vesicle production in not only gram-negative but also gram-positive bacteria. | many gram-negative bacteria naturally produce membrane vesicles (mvs) to the extracellular milieu. the pseudomonas quinolone signal (pqs), a quorum-sensing signal of pseudomonas aeruginosa, is a positive regulator of mv production. in this study, we investigated its effects on mv production in other gram-negative and -positive bacterial species. the addition of pqs to an escherichia coli k12 culture resulted in increased mv production and enlarged mvs. an excessive amount of mgcl(2) repressed e. ... | 2010 | 21576862 |
| the structure of a burkholderia pseudomallei immunophilin-inhibitor complex reveals new approaches to antimicrobial development. | macrophage infectivity potentiators (mips) are a subset of immunophilins associated with virulence in a range of micro-organisms. these proteins possess prolyl-peptide isomerase activity and are inhibited by drugs including rapamycin and tacrolimus. we determined the structure of the mip homologue (bpml1) from the human pathogen and biowarfare threat burkholderia pseudomallei by nuclear magnetic resonance and x-ray crystallography. the crystal structure suggests that key catalytic residues in th ... | 2011 | 21574961 |
| production and characterization of chimeric monoclonal antibodies against burkholderia pseudomallei and b. mallei using the dhfr expression system. | burkholderia pseudomallei (bp) and b. mallei (bm) are closely related gram-negative, facultative anaerobic bacteria which cause life-threatening melioidosis in human and glanders in horse, respectively. our laboratory has previously generated and characterized more than 100 mouse monoclonal antibodies (mabs) against bp and bm, according to in vitro and in vivo assay. in this study, 3 mabs (bp7 10b11, bp7 2c6, and bp1 7f7) were selected to develop into chimeric mouse-human monoclonal antibodies ( ... | 2011 | 21573027 |
| pulmonary melioidosis in cambodia: a prospective study. | abstract: background: melioidosis is a disease caused by burkholderia pseudomallei and considered endemic in south-east asia but remains poorly documented in cambodia. we report the first series of hospitalized pulmonary melioidosis cases identified in cambodia describing clinical characteristics and outcomes. methods: we characterized cases of acute lower respiratory infections (alri) that were identified through surveillance in two provincial hospitals. severity was defined by systolic blood p ... | 2011 | 21569563 |
| the response of pseudomonas aeruginosa pao1 efflux pump-defective mutants to n-octanoyl-l-homoserine lactone. | n-octanoyl-l-homoserine lactone (c8-hsl) is an acyl-homoserine-lactone signal utilized in the quorum-sensing (qs) systems of burkholderia cenocepacia and other bacterial species. although also produced by pseudomonas aeruginosa, its role in this species has not been elucidated. here, we report that c8-hsl modulated antibiotic resistance and pyocyanin production in a p. aeruginosa efflux pump-deficient mutant. the rhl/las quorum-sensing system and qscr gene were both shown to be nonessential in t ... | 2009 | 21566394 |
| new dimensions at molecular microbiology: 3d graphics and the wiley online library. | 2011 | 21564330 | |
| bacteria contributing to behaviour of radiocarbon in sodium acetate. | an acetate-utilising bacterium was isolated and identified from deionised water that was used for flooding of paddy soils in this study's batch culture experiments. bacteria in the deionised water samples formed colonies on agar plates containing [1,2-(14)c] sodium acetate, and the autoradiograms showed that all the colonies were positive for (14)c utilisation. then one of the acetate-utilising bacteria was isolated. the isolate was characterised by phylogenetic analysis, cell morphology, gram s ... | 2011 | 21561944 |
| isolation of effective 3-chlorobenzoate-degraders in soil using community analyses by pcr-dgge. | the screening of pollutant degraders by relying solely on cultivation techniques such as liquid enrichment often fails to isolate the actual degraders in the environment. community analyses by pcr-denaturing gradient gel electrophoresis (dgge) were performed to isolate bacteria that can degrade 3-chlorobenzoate (3cb) effectively in soil. a forest soil sample was repeatedly dosed with 3cb (500 mg kg(-1)) to enrich it with indigenous 3cb-degraders, and changes in the bacterial community were monit ... | 2008 | 21558720 |
| suppression of bacterial wilt and fusarium wilt by a burkholderia nodosa strain isolated from kalimantan soils, indonesia. | a trial was conducted to suppress bacterial wilt of tomato (bwt) caused by ralstonia solanacearum using biocontrol agents (bcas) isolated from soils in kalimantan, indonesia. five isolates were selected from 270 isolates as better performing bcas through screening four times using a pumice medium. the isolates selected were identified as burkholderia nodosa, burkholderia sacchari, burkholderia pyrrocinia and burkholderia terricola according to 16s rdna sequences, fatty acid composition and carbo ... | 2008 | 21558699 |
| endophytic bacteria in the rice plant. | endophytic bacteria are defined as bacteria detected inside surface-sterilized plants or extracted from inside plants and having no visibly harmful effects on the plants. various kinds of endophytic bacteria, such as pantoea, methylobacterium, azospirillum, herbaspirillum, burkholderia and rhizobium etc., have been found inside rice plants. this minireview summarizes and discusses recent studies of endophytic bacteria residing in rice plants, focusing on flora, origin, movement, and interaction ... | 2008 | 21558696 |
| melioidosis: a clinical overview. | introduction melioidosis, an infection caused by the environmental gram-negative bacillus burkholderia pseudomallei, has emerged as an important cause of morbidity and mortality in southeast asia and northern australia. sources of data a review of the literature using pubmed. areas of agreement approaches to diagnosis and antimicrobial therapy. areas of controversy whether seroconversion signals the presence of a quiescent bacterial focus and an increase in long-term risk of melioidosis. areas t ... | 2011 | 21558159 |
| burkholderia pseudomallei-induced expression of a negative regulator, sterile-{alpha} and armadillo motif (sarm) containing protein, in mouse macrophages: a possible mechanism for suppression of myd88-independent pathway. | burkholderia pseudomallei, a causative agent of melioidosis, is a gram-negative facultative intracellular bacterium that can survive and multiply in macrophages. previously we demonstrated that b. pseudomallei failed to activate gene expression downstream of myd88-independent pathway, particularly the expression of ifn-β and inducible nitric oxide synthase (inos), leading to the inability of macrophages to kill this bacterium. in the present report, we extended our study to show that b. pseudoma ... | 2011 | 21555400 |
| crystal structures of burkholderia cenocepacia dihydropteroate synthase in the apo-form and complexed with the product 7,8-dihydropteroate. | abstract: | 2011 | 21554707 |
| molecular assembly-assisted biocatalytic reactions in ionic liquids. | room temperature ionic liquids (rtils), having no measurable vapor pressure, represent an interesting class of tunable designer solvents. due to their many unique properties, ils have been used as attractive alternatives to environmentally harmful ordinary organic solvents in a wide range of applications including enzymatic biotransformation. compared to conventional organic solvents, ils offer many advantages for biocatalysis such as enhanced conversion rates, high enantioselectivity, better en ... | 2011 | 21553181 |
| complete genome sequence of the plant growth-promoting endophyte burkholderia phytofirmans strain psjn. | burkholderia phytofirmans psjn(t) is able to efficiently colonize the rhizosphere, root and above-ground plant tissues of a wide variety of genetically unrelated plants such as potato, canola, maize and grapevine. strain psjn shows strong plant growth-promoting effects and was reported to enhance plant vigour and resistance to biotic and abiotic stresses. here, we report the genome sequence of this strain, which indicates the presence of multiple traits relevant for endophytic colonization and p ... | 2011 | 21551308 |
| lux-marking and application of carbofuran degrader burkholderia cepacia pcl3. | a luxab-mutant of the carbofuran degrading bacterium burkholderia cepacia pcl3 was successfully constructed with the capability to emit a luminescence signal of 1.6×10(-3)rlucfu(-1). the mutant has a growth pattern and carbofuran degradation ability similar to pcl3 wild-type. the luminescent emission by pcl3:luxab1 directly correlated with the metabolic activity of the cells. the optimal ph, temperature and n-decanal concentration for luminescence emission are 7.0, 35°c and 0.01%, respectively. ... | 2011 | 21549227 |
| a case of native valve endocarditis caused by burkholderia cepacia without predisposing factors. | abstract: background: infective endocarditis is rarely caused by burkholderia cepacia. this infection is known to occur particularly in immunocompromised hosts, intravenous heroin users, and in patients with prosthetic valve replacement. most patients with burkholderia cepacia endocarditis usually need surgical treatment in addition to antimicrobial treatment. case presentation : here, we report the case of a patient who developed burkholderia cepacia-induced native valve endocarditis with conse ... | 2011 | 21548997 |
| methods to classify bacterial pathogens in cystic fibrosis. | many bacteria can be detected in cf sputum, pathogenic and commensal. modified koch's criteria for identification of established and emerging cf pathogens are therefore described. methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from cf patients by means of conventional culturing and staining techniques and by the pna fish technique. additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a f ... | 2011 | 21547731 |
| improved culture-based detection and quantification of burkholderia pseudomallei from soil. | environmental surveillance of the gram-negative soil bacterium burkholderia pseudomallei, the aetiological agent of melioidosis, is important in order to define human populations and livestock at risk of acquiring the infection. this study aimed to develop a more sensitive method for the detection of b. pseudomallei from soil samples in endemic areas compared with the currently used culture method based on soil dispersion in water. we report the development of a new protocol that involves soil d ... | 2011 | 21543099 |
| chemoenzymatic synthesis of (2r,3r,4r)-dehydroxymethylepoxyquinomicin (dhmeq), a new activator of antioxidant transcription factor nrf2. | dehydroxymethylepoxyquinomicin (dhmeq, 1a) is a specific and potent inhibitor of nf-κb, and it is now being developed as an anti-inflammatory and anticancer agent. while previously only the (2s,3s,4s)-form had been available from the racemate by using lipase-catalyzed enantioselective resolution, in the present study a new route for production of the (2r,3r,4r)-form was established by use of a chemoenzymatic approach. (1r*,2r*,3r*)-2,3-epoxy-5-n-[(2-hydroxybenzoyl)amino]-4,4-dimethoxycyclohex-5- ... | 2011 | 21541373 |
| endophytic bacteria in toxic south african plants: identification, phylogeny and possible involvement in gousiekte. | south african plant species of the genera fadogia, pavetta and vangueria (all belonging to rubiaceae) are known to cause gousiekte (literally 'quick disease'), a fatal cardiotoxicosis of ruminants characterised by acute heart failure four to eight weeks after ingestion. noteworthy is that all these plants harbour endophytes in their leaves: nodulating bacteria in specialized nodules in pavetta and non-nodulating bacteria in the intercellular spaces between mesophyll cells in fadogia and vangueri ... | 2011 | 21541284 |
| evolution of an endofungal lifestyle: deductions from the burkholderia rhizoxinica genome. | abstract: | 2011 | 21539752 |
| the pseudomonas quinolone signal (pqs), and its precursor hhq, modulate interspecies and interkingdom behaviour. | the pseudomonas quinolone signal (pqs), and its precursor 2-heptyl-4-quinolone (hhq), play a key role in coordinating virulence in the important cystic fibrosis pathogen pseudomonas aeruginosa. the discovery of hhq analogues in burkholderia and other microorganisms led us to investigate the possiblity that these compounds can influence interspecies behaviour. we found that surface-associated phenotypes were repressed in gram-positive and gram-negative bacteria as well as in pathogenic yeast in r ... | 2011 | 21539583 |
| diversity analysis of burkholderia cepacia complex in the water bodies of west lake, hangzhou, china. | a survey of burkholderia cepacia complex (bcc) species was conducted in water bodies of west lake in china. a total of 670 bacterial isolates were recovered on selective media. out of them, 39.6% (265 isolates) were assigned to the following species: burkholderia multivorans, burkholderia cenocepacia reca lineage iiia, iiib, burkholderia stabilis, burkholderia vietnamiensis, and burkholderia seminalis while b. cenocepacia is documented as a dominant bcc species in water of west lake. in addition ... | 2011 | 21538256 |
| improved antibiotic resistance gene cassette for marker exchange mutagenesis in ralstonia solanacearum and burkholderia species. | marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. new plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in ralstonia solanacearum and many antibiotic-resistant burkholderia spp. insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene r ... | 2011 | 21538255 |
| adaptation and antibiotic tolerance of anaerobic burkholderia pseudomallei. | the gram-negative bacterium, burkholderia pseudomallei, is the etiological agent of melioidosis and is remarkably resistant to most classes of antibacterials. even after months of treatment with antibacterials relatively effective in vitro, there is a high rate of treatment failure, indicating that this pathogen alters its patterns of antibacterial susceptibility in response to cues encountered in the host. the pathology of melioidosis indicates that b. pseudomallei encounter host microenvironme ... | 2011 | 21537012 |
| burkholderia cenocepacia phenotypic clonal variation during three and a half years of residence in the lungs of a cystic fibrosis patient. | chronic lung infection is the major cause of morbidity and premature mortality in cystic fibrosis (cf) patients. bacteria of the burkholderia cepacia complex are the most threatening pathogens in cf and a better understanding of how these bacteria adapt to the cf airway environment and resist to the host defense mechanisms and to therapeutically administered antibiotics is crucial. to provide clues on the adaptive strategies adopted by b. cenocepacia during long-term colonization, we carried out ... | 2011 | 21536796 |
| transcript amplification from single bacterium for transcriptome analysis. | total transcript amplification (tta) from single eukaryotic cells for transcriptome analysis is established, but tta from a single prokaryotic cell presents additional challenges with much less starting material, the lack of poly(a)-tails, and the fact that the messages can be polycistronic. here, we describe a novel method for single-bacterium tta using a model organism, burkholderia thailandensis, exposed to a subinhibitory concentration of the antibacterial agent, glyphosate. utilizing a b. t ... | 2011 | 21536723 |
| specific developmental window for establishment of an insect-microbe gut symbiosis. | the alydid stinkbug riptortus pedestris is specifically associated with a beneficial burkholderia symbiont in the midgut crypts. exceptional among insect-microbe mutualistic associations, the burkholderia symbiont is not vertically transmitted but orally acquired by nymphal insects from the environment every generation. here we experimentally investigated the process of symbiont acquisition during the nymphal development of r. pedestris. in a field population, many 2(nd) instar nymphs were burkh ... | 2011 | 21531836 |
| isolation of burkholderia cenocepacia j 2315 from non-cystic fibrosis pediatric patients in india. | we report for the first time 2 cases of multidrug-resistant burkholderia cenocepacia j2315 isolated from blood samples of patients without cystic fibrosis from a pediatric unit in a hospital in india. the first patient presented with community-acquired bacteremia, and the second patient was immunocompromised and developed hospital-acquired infection approximately 17 days after admission. the isolates from both patients were multidrug-resistant and strong biofilm producers. surveillance cultures ... | 2011 | 21531270 |
| transport and delivery of antimicrobial agents in burkholderia biofilms. | 2010 | 21529610 | |
| bacterial leaf symbiosis in ardisia (myrsinoideae, primulaceae): molecular evidence for host specificity. | the association between bacteria and leaves in ardisia has been described as a cyclic and obligate symbiosis in which bacteria are maintained throughout all stages of the plant's life cycle to guarantee normal growth and survival of the host. this intimate interaction suggests that both partners have co-diversified together. to test this co-speciation hypothesis, we constructed an endosymbiont (16s rdna and gyrb) and host (rps16, trnl, matk and its) phylogeny. phylogenetic analyses of the endosy ... | 2011 | 21527340 |
| deciphering the role of rnd efflux transporters in burkholderia cenocepacia. | burkholderia cenocepacia j2315 is representative of a highly problematic group of cystic fibrosis (cf) pathogens. eradication of b. cenocepacia is very difficult with the antimicrobial therapy being ineffective due to its high resistance to clinically relevant antimicrobial agents and disinfectants. rnd (resistance-nodulation-cell division) efflux pumps are known to be among the mediators of multidrug resistance in gram-negative bacteria. since the significance of the 16 rnd efflux systems prese ... | 2011 | 21526150 |
| metagenomic analysis of the 1-aminocyclopropane-1-carboxylate deaminase gene (acds) operon of an uncultured bacterial endophyte colonizing solanum tuberosum l. | deamination of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (acc) is a key plant-beneficial trait found in many plant growth-promoting bacteria. in this study, we analysed acc deaminase genes (acds) of bacterial endophytes colonizing field-grown potato plants. pcr analysis revealed the presence of two types of acds genes, the dominant one showing high homology to an acds gene derived from pseudomonas fluorescens. construction, functional screening and sequence analysis of metagen ... | 2011 | 21523387 |
| identification of unknown ocular pathogens in clinically suspected eye infections using ribosomal rna gene sequence analysis. | clin microbiol infect 2011; 17: 776-779 abstract: dna sequence-based identification of pathogens from ocular samples of patients with clinically suspected eye infections was accomplished using 16s and internal transcribed spacer (its) ribosomal rna gene sequence analysis. pcr was positive for 24 of 99 samples tested. both culture and 16s rdna sequence analysis identified pseudomonas aeruginosa, streptococci and enterobacteriaceae. isolates misidentified as burkholderia cepacia by biochemical tes ... | 2011 | 21521414 |
| mycotic aneurysm caused by burkholderia pseudomallei: report of a brazilian strain genetically related to thai strains. | clin microbiol infect 2011; 17: 719-721 abstract: melioidosis, a severe infectious disease caused by burkholderia pseudomallei that is prevalent in southeast asia and northern australia, has been sporadically reported in brazil since 2003. we report a case of aortic aneurysm with blood culture positive for b. pseudomallei. the phylogenetic analysis of 16s ribosomal dna showed this isolate to be evolutionarily grouped with the mshr346 strains from thailand. | 2011 | 21521412 |
| genomic analysis of the phenylacetyl-coa pathway in burkholderia xenovorans lb400. | the phenylacetyl-coa (paa) catabolic pathway and genome-wide gene expression responses to phenylacetate catabolism were studied in the polychlorinated biphenyl (pcb)-degrading strain burkholderia xenovorans lb400. microarray and rt-qpcr analyses identified three non-contiguous chromosomal clusters of genes that are predicted to encode a complete paa pathway that were induced up to 40-fold during growth of lb400 on phenylacetate: paaghijkr, paaanebdf, and paac. comparison of the available genome ... | 2011 | 21519854 |
| [effect of probiotic bacteria on survival and growth of cortez oyster larvae, crassostrea corteziensis (bivalvia: ostreidae)]. | disease control problems have major constraints in aquaculture production, and the use of probiotics in larviculture is a valid alternative to antibiotics. this study analyzed the effect of probiotic bacteria on survival and final size of cortez oyster larvae crassostrea corteziensis. two different probiotic concentrations were evaluated, 1 x 10(4) and 1 x 10(5) cfu/ml of lactic acid bacteria (strain ns61) isolated from nodipecten subnodosus, and bacilli isolated from the white leg shrimp, litop ... | 2011 | 21516645 |
| phenylalanine induces burkholderia cenocepacia phenylacetic acid catabolism through degradation to phenylacetyl-coa in synthetic cystic fibrosis sputum medium. | synthetic cystic fibrosis sputum medium (scfm) is rich in amino acids and supports robust growth of burkholderia cenocepacia, a member of the burkholderia cepacia complex (bcc). previous work demonstrated that b. cenocepacia phenylacetic acid (pa) catabolic genes are up-regulated during growth in scfm and are required for full virulence in a caenorhabditis elegans host model. in this work, we investigated the role of phenylalanine, one of the aromatic amino acids present in scfm, as an inducer o ... | 2011 | 21511027 |
| disseminated burkholderia gladioli infection in a lung transplant recipient with underlying hypocomplementemic urticarial vasculitis. | g.r. thompson iii, b.l. wickes, m.l. herrera, t.c. haman, j.s. lewis ii, j.h. jorgensen. disseminated burkholderia gladioli infection in a lung transplant recipient with underlying hypocomplementemic urticarial vasculitis. transpl infect dis 2011. all rights reserved abstract: burkholderia gladioli is difficult to definitively identify within the laboratory using phenotypic testing alone. we describe a case of recurrent b. gladioli infection in a lung transplant recipient with underlying hypocom ... | 2011 | 21504528 |
| elucidating the reaction mechanism of the benzoate oxidation pathway encoded aldehyde dehydrogenase from burkholderia xenovorans lb400. | oxidation of cis-3,4-dehydroadipyl-coa semialdehyde to cis-3,4-dehydroadipyl-coa by the aldehyde dehydrogenase, aldh(c) (ec.1.2.1.77), is an essential step in the metabolism of benzoate in burkholderia xenovorans lb400. in a previous study, we established a structural blueprint for this novel group of aldh enzymes. here, we build significantly on this initial work and propose a detailed reaction mechanism for aldh(c) based on comprehensive structural and functional investigations of active site ... | 2011 | 21495107 |
| the airway microbiome in cystic fibrosis and implications for treatment. | lung disease in cystic fibrosis (cf) results from chronic airway infection and inflammation leading to progressive bronchiectasis and respiratory failure. bacterial pathogens, including pseudomonas aeruginosa, staphylococcus aureus, and burkholderia cepacia, are known contributors. recent studies using culture-independent molecular techniques and anaerobic cultures have broadened our view of cf airway bacterial communities. | 2011 | 21494150 |
| molecular phylogeny of burkholderia pseudomallei from a remote region of papua new guinea. | the island of new guinea is located midway between the world's two major melioidosis endemic regions of australia and southeast asia. previous studies in papua new guinea have demonstrated autochthonous melioidosis in balimo, western province. in contrast to other regions of endemicity, isolates recovered from both environmental and clinical sources demonstrate narrow genetic diversity over large spatial and temporal scales. | 2011 | 21483841 |
| subsurface examination of a foliar biofilm using scanning electron- and focused-ion-beam microscopy. | the dual beam scanning electron microscope, equipped with both a focused ion- and scanning electron-beam (fib sem) is a novel tool for the exploration of the subsurface structure of biological tissues. the fib can remove a predetermined amount of material from a selected site to allow for subsurface exploration and when coupled with sem or scanning ion-beam microscopy (sim) could be suitable to examine the subsurface structure of bacterial biofilms on the leaf surface. the suitability of chemica ... | 2011 | 21482121 |
| comparative in vitro susceptibility of burkholderia pseudomallei to doripenem, ertapenem, tigecycline and moxifloxacin. | burkholderia pseudomallei, the causative agent of melioidosis, continues to present therapeutic challenges in endemic areas. a number of clinical issues have prompted consideration of alternative antimicrobial therapies. these include stability in 24-h infusion pumps, broad-spectrum coverage in the empirical treatment of community-acquired pneumonia, cost, the need for effective oral agents and rare reports of emerging resistance. this study aimed to examine the in vitro susceptibility of b. pse ... | 2011 | 21481571 |
| burkholderia cepacia endophthalmitis: clinico-microbiologic profile and outcomes. | background:: to report the clinical presentations, antibiotic sensitivities, and outcomes of burkholderia cepacia endophthalmitis. methods:: retrospective, consecutive, noncomparative, interventional case series. we reviewed case records of culture-proven b. cepacia endophthalmitis from the endophthalmitis registry between january 2003 and december 2008. data collected included the cause of endophthalmitis, time of presentation, presenting visual acuity, initial surgical procedures performed, cl ... | 2011 | 21478807 |
| complete genome sequence of burkholderia gladioli bsr3. | we report the complete genome sequence of burkholderia gladioli bsr3, isolated from a diseased rice sheath in south korea. | 2011 | 21478339 |
| a chemically modified lipase preparation for catalyzing the transesterification reaction in even highly polar organic solvents. | acylation of pseudomonas cepacia lipase with pyromellitic dianhydride to modify 72% of total amino groups was carried out. different organic solvents were screened for precipitation of modified lipase. it was found that 1,2-dimethoxyethane was the best precipitant which precipitated 97% protein and complete activity. pcmc (protein coated microcrystals), clpcmc (crosslinked protein coated microcrystals), epros (enzyme precipitated and rinsed with organic solvents) and ph tuned preparations of mod ... | 2011 | 21463943 |
| a psychrotrophic burkholderia cepacia strain isolated from refrigerated raw milk showing proteolytic activity and adhesion to stainless steel. | the proteolytic activity of a psychrotrophic strain of burkholderia cepacia isolated from refrigerated raw milk was characterized. bur. cepacia produced proteolytic activity during growth at refrigeration temperature, with maximum activity at ph 6-7. the enzyme showed relative thermal stability in the range 40-50°c during 25 min, and maintained 80% its initial activity at 76°c/30 s. milk coagulation assay showed that the crude protease from bur. cepacia caused coagulation from day 2 for skimmed ... | 2011 | 21457588 |
| ligand binding induces an ammonia channel in 2-amino-2-desoxyisochorismate (adic) synthase phze. | phze utilizes chorismate and glutamine to synthesize 2-amino-2-desoxyisochorismate (adic) in the first step of phenazine biosynthesis. the phze monomer contains both a chorismate-converting menaquinone, siderophore, tryptophan biosynthesis (mst) and a type 1 glutamine amidotransferase (gatase1) domain connected by a 45-residue linker. we present here the crystal structure of phze from burkholderia lata 383 in a ligand-free open and ligand-bound closed conformation at 2.9 and 2.1 å resolution, re ... | 2011 | 21454481 |
| burkholderia glumae: next major pathogen of rice? | burkholderia glumae causes bacterial panicle blight of rice, which is an increasingly important disease problem in global rice production. toxoflavin and lipase are known to be major virulence factors of this pathogen, and their production is dependent on the tofi/tofr quorum-sensing system, which is mediated by n-octanoyl homoserine lactone. flagellar biogenesis and a type iii secretion system are also required for full virulence of b. glumae. bacterial panicle blight is thought to be caused by ... | 2011 | 21453428 |
| in vitro and in vivo studies of monoclonal antibodies with prominent bactericidal activity against burkholderia pseudomallei and burkholderia mallei. | our laboratory has developed more than a hundred mouse monoclonal antibodies (mabs) against burkholderia pseudomallei and burkholderia mallei. these antibodies have been categorized into different groups based on their specificities and the biochemical natures of their target antigens. the current study first examined the bactericidal activities of a number of these mabs by an in vitro opsonic assay. then, the in vivo protective efficacy of selected mabs was evaluated using balb/c mice challenge ... | 2011 | 21450976 |
| vith world melioidosis congress report: celebrating a century of research. | 2011 | 21449838 | |
| differences in strategies to combat osmotic stress in burkholderia cenocepacia elucidated by nmr-based metabolic profiling. | to investigate mechanisms of osmotic tolerance in burkholderia cenocepacia, a member of the b. cepacia complex (bcc) of closely related strains, which is of clinical as well as environmental importance. | 2011 | 21446999 |
| [bacteriocins and bacteriophages of pathogenic burkholderia]. | detection of bacteriocins and phages in pathogenic bacteria of burkholderia genus and study of their specificity range. | 2011 | 21446163 |
| [identification of bacterial contamination in liquid soap for hospital use]. | this study performed a bacteriological analysis of the liquid soap in dispensers that health professionals use for hand washing. this exploratory, cross-sectional study was developed at the hospitalization units of a medium-sized hospital in fortaleza, ceará, brazil. data were collected between may and july 2007. fifty-nine liquid soap dispensers were analyzed, of which 33 contained the following microorganisms: burkholderia cepacia (14), pseudomonas putidas (9), pseudomonas aeruginosa (3), kleb ... | 2011 | 21445502 |
| biodiesel production in packed-bed reactors using lipase-nanoparticle biocomposite. | the development of appropriate reactors is crucial for the production of biodiesel. in this study, a packed-bed reactor system using lipase-fe(3)o(4) nanoparticle biocomposite catalyst was successfully developed for biodiesel production based on soybean oil methanolysis. emulsification before methanolysis improved the reaction rate. the lipase-nanoparticle biocomposite showed high activity and stability in the single-packed-bed reactor at an optimal flow rate (0.25 ml min(-1)). after 240 h of re ... | 2011 | 21435865 |
| marr homologs with urate-binding signature. | bacteria that associate with living hosts require intricate mechanisms to detect and respond to host defenses. part of the early host defense against invading bacteria is the production of reactive oxygen species, and xanthine oxidase is one of the main producers of such agents. the end-product of the enzymatic activity of xanthine oxidase, urate, was previously shown to be the natural ligand for deinococcus radiodurans-encoded hucr and it was shown to attenuate dna binding by agrobacterium tume ... | 2011 | 21432936 |
| impact of chlorophenols on microbiota of an unpolluted acidic soil: microbial resistance and biodegradation. | the impact of 2-monochlorophenol (mcp), 2,4,6-trichlorophenol (tcp) and pentachlorophenol (pcp) on the microbial community of an acidic forest soil was studied under controlled laboratory conditions by spiking microcosms with the pollutants at concentrations ranging from 0.1 to 5000 mg kg(-1) . a decrease in the cumulative respirometric values and changes in the bacterial and fungal community composition were detected at 1000 mg mcp kg(-1) , 100 mg tcp kg(-1) and 100 and 1000 mg pcp kg(-1) . how ... | 2011 | 21426365 |
| rice root-associated bacteria: insights into community structures across 10 cultivars. | in this study, the effects of plant genotype, soil type and nutrient use efficiency on the composition of different bacterial communities associated with rice roots were investigated. thus, total bacteria, alpha- and betaproteobacteria, pseudomonas and actinobacteria were studied using pcr, followed by denaturing gradient gel electrophoresis (pcr-dgge). rice genotype determined, to a large extent, the composition of the different bacterial communities across cultivars. several cultivars belongin ... | 2011 | 21426364 |
| the homogentisate and homoprotocatechuate central pathways are involved in 3- and 4-hydroxyphenylacetate degradation by burkholderia xenovorans lb400. | genome characterization of the model pcb-degrading bacterium burkholderia xenovorans lb400 revealed the presence of eleven central pathways for aromatic compounds degradation, among them, the homogentisate and the homoprotocatechuate pathways. however, the functionality of these central pathways in strain lb400 has not been assessed and related peripheral pathways has not been described. | 2011 | 21423751 |
| quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. | although the exact role of quorum sensing (qs) in various stages of biofilm formation, maturation, and dispersal and in biofilm resistance is not entirely clear, the use of qs inhibitors (qsi) has been proposed as a potential antibiofilm strategy. we have investigated whether qsi enhance the susceptibility of bacterial biofilms to treatment with conventional antimicrobial agents. the qsi used in our study target the acyl-homoserine lactone-based qs system present in pseudomonas aeruginosa and bu ... | 2011 | 21422204 |
| development of reagents and assays for the detection of pathogenic burkholderia species. | rapid detection of the category b biothreat agents burkholderia pseudomallei and burkholderia mallei in acute infections is critical to ensure that appropriate treatment is administered quickly to reduce an otherwise high probability of mortality (ca. 40% for b. pseudomallei). we are developing assays that can be used in clinical laboratories or security applications for the direct detection of surface-localized and secreted macromolecules produced by these organisms. we present our current medi ... | 2011 | 21413172 |
| the burkholderia pseudomallei type iii secretion system and bopa are required for evasion of lc3-associated phagocytosis. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal infectious disease endemic in tropical regions worldwide, and especially prevalent in southeast asia and northern australia. this intracellular pathogen can escape from phagosomes into the host cytoplasm, where it replicates and infects adjacent cells. we previously demonstrated that, in response to b. pseudomallei infection of macrophage cell line raw 264.7, a subset of bacteria co-localized with the autophagy marker prote ... | 2011 | 21412437 |
| distinct roles for nitric oxide in resistant c57bl/6 and susceptible balb/c mice to control burkholderia pseudomallei infection. | burkholderia pseudomallei is the causative agent of melioidosis, an emerging bacterial infectious disease in tropical and subtropical areas. we recently showed that nadph oxidase but not nitric oxide (no) contributes to resistance in innately resistant c57bl/6 mice in a b. pseudomallei respiratory infection model. however, the function of no for resistance was shown to differ among distinct strains of mice and proved also to be stage dependent in various infection models. the present study there ... | 2011 | 21410970 |
| high abundance and role of antifungal bacteria in compost-treated soils in a wildfire area. | compost has been widely used in order to promote vegetation growth in post-harvested and burned soils. the effects on soil microorganisms were scarcely known, so we performed the microbial analyses in a wildfire area of the taebaek mountains, korea, during field surveys from may to september 2007. using culture-dependent and -independent methods, we found that compost used in burned soils influenced a greater impact on soil fungi than bacteria. compost-treated soils contained higher levels of an ... | 2011 | 21409344 |
| complete killing of caenorhabditis elegans by burkholderia pseudomallei is dependent on prolonged direct association with the viable pathogen. | burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. much remains to be known about the contributions of genotypic variations within the bacteria and the host, and environmental factors that lead to the manifestation of the clinical symptoms of melioidosis. | 2011 | 21408228 |
| dictyostelium discoideum as a model system for identification of burkholderia pseudomallei virulence factors. | burkholderia pseudomallei is an emerging bacterial pathogen and category b biothreat. human infections with b. pseudomallei (called melioidosis) present as a range of manifestations, including acute septicemia and pneumonia. although melioidosis can be fatal, little is known about the molecular basis of b. pseudomallei pathogenicity, in part because of the lack of simple, genetically tractable eukaryotic models to facilitate en masse identification of virulence determinants or explore host-patho ... | 2011 | 21402765 |
| dna fingerprinting of human isolates of burkholderia pseudomallei from different geographical regions of malaysia. | melioidosis is an infectious disease caused by burkholderia pseudomallei and endemic in southeast asia. one hundred and forty six clinical isolates of b. pseudomallei from different states in malaysia were obtained and molecular typing was carried out using pulsed-field gel electrophoresis (pfge). overall, nine clusters were successfully identified. burkholderia pseudomallei isolates used in this study were found to be genetically diverse and there were differences in the clusters of isolates fr ... | 2010 | 21399594 |
| [lymphohistiocytic activation syndrome and burkholderia cepacia complex infection in a child revealing chronic granulomatous disease and chromosomal integration of the hhv-6 genome]. | chronic granulomatous disease (gcd) is characterized by severe infections, notably with burkholderia cepacia complex (bcc). gcd is rarely complicated by lymphohistiocytic activation syndromes, most often secondary to bacterial or viral infections, in particular human herpes virus 6 (hhv-6). we describe the case of a 10-month-old boy who suffered from multiple organ failure due to a bcc infection and a lymphohistiocytic activation syndrome, leading to diagnosis of gcd. the initial search for hhv- ... | 2011 | 21397473 |
| the antibacterial threaded-lasso peptide capistruin inhibits bacterial rna polymerase. | capistruin, a ribosomally synthesized, post-translationally modified peptide produced by burkholderia thailandensis e264, efficiently inhibits growth of burkholderia and closely related pseudomonas strains. the functional target of capistruin is not known. capistruin is a threaded-lasso peptide (lariat peptide) consisting of an n-terminal ring of nine amino acids and a c-terminal tail of 10 amino acids threaded through the ring. the structure of capistruin is similar to that of microcin j25 (mcc ... | 2011 | 21396375 |
| mechanism and inhibition of the fabi enoyl-acp reductase from burkholderia pseudomallei. | as an initial step in developing novel antibacterials against burkholderia pseudomallei, we have characterized the fabi enoyl-acp reductase homologues in the type ii fatty acid biosynthesis pathway from this organism and performed an initial enzyme inhibition study. | 2011 | 21393229 |
| clinical characteristics and outcomes of patients with burkholderia cepacia bacteremia in an intensive care unit. | the purpose of this study was to investigate a cohort of patients with burkholderia cepacia bacteremia in the intensive care unit (icu) at our institution. a large outbreak of b. cepacia bacteremia involving 95 patients lasted for 4 years in an icu in northern taiwan. the clinical characteristics and antimicrobial treatment responses of these patients were analyzed. minimal inhibitory concentrations were determined and pulse-field gel electrophoresis was performed for the 73 available isolates. ... | 2011 | 21392926 |
| outer membrane proteome of burkholderia pseudomallei and burkholderia mallei from diverse growth conditions. | burkholderia mallei and burkholderia pseudomallei are closely related, aerosol-infective human pathogens that cause life-threatening diseases. biochemical analyses requiring large-scale growth and manipulation at biosafety level 3 under select agent regulations are cumbersome and hazardous. we developed a simple, safe, and rapid method to prepare highly purified outer membrane (om) fragments from these pathogens. shotgun proteomic analyses of oms by trypsin shaving and mass spectrometry identifi ... | 2011 | 21391724 |
| a novel concept combining experimental and mathematical analysis for the identification of unknown interspecies effects in a mixed culture. | bacteria in natural habitats only occur in consortia together with various other species. characterization of bacterial species, however, is normally done by laboratory testing of pure isolates. any interactions that might appear during growth in mixed-culture are obviously missed by this approach. existing experimental studies mainly focus on two-species mixed cultures with species specifically chosen for their known growth characteristics, and their anticipated interactions. various theoretica ... | 2011 | 21391206 |
| defining a structural and kinetic rationale for paralogous copies of phenylacetate-coa ligases from the cystic fibrosis pathogen burkholderia cenocepacia j2315. | the phenylacetic acid (paa) degradation pathway is the sole aerobic route for phenylacetic acid metabolism in bacteria and facilitates degradation of environmental pollutants such as styrene and ethylbenzene. the paa pathway also is implicated in promoting burkholderia cenocepacia infections in cystic fibrosis patients. intriguingly, the first enzyme in the paa pathway is present in two copies (paak1 and paak2), yet each subsequent enzyme is present in only a single copy. furthermore, sequence d ... | 2011 | 21388965 |
| burkholderia fungorum dbt1: a promising bacterial strain for bioremediation of pahs-contaminated soils. | an extensive taxonomic analysis of the bacterial strain burkholderia sp. dbt1, previously isolated from an oil refinery wastewater drainage, is discussed here. this strain is capable of transforming dibenzothiophene through the 'destructive' oxidative pathway referred to as the kodama pathway. burkholderia dbt1 has also been proved to use fluorene, naphthalene and phenanthrene as carbon and energy sources, although growth on the first two compounds requires a preinduction step. this evidence sug ... | 2011 | 21388438 |
| characterization of n-acylhomoserine lactone-degrading bacteria associated with the zingiber officinale (ginger) rhizosphere: co-existence of quorum quenching and quorum sensing in acinetobacter and burkholderia. | cell-to-cell communication (quorum sensing (qs)) co-ordinates bacterial behaviour at a population level. consequently the behaviour of a natural multi-species community is likely to depend at least in part on co-existing qs and quorum quenching (qq) activities. here we sought to discover novel n-acylhomoserine lactone (ahl)-dependent qs and qq strains by investigating a bacterial community associated with the rhizosphere of ginger (zingiber officinale) growing in the malaysian rainforest. | 2011 | 21385437 |
| a colorimetric assay for determination of methyl parathion using recombinant methyl parathion hydrolase. | a simple, rapid and sensitive colorimetric dipstick assay for the detection of the organophosphorous insecticide methyl parathion (mpt) residue in vegetables was developed. the assay was based on the hydrolysis of mpt by a recombinant methyl parathion hydrolase (recmph), the encoding gene of which was isolated from burkholderia cepacia, a soil bacterium indigenous to thailand. this reaction generates protons leading to a change in ph that correlates with the amount of mph present. hence, the ph ... | 2011 | 21381204 |
| systemic melioidiosis. | 2011 | 21378433 | |
| identification of the bacterial endosymbionts in leaf nodules of pavetta (rubiaceae). | three genera in rubiaceae (pavetta, psychotria and sericanthe) harbour bacterial endosymbionts within leaf nodules or galls. the present paper identifies the bacterial endophytes in three leaf nodulating pavetta species. in order to reveal their identity and assess their phylogenetic position, dna data from 16s rdna, reca and gyrb genes were sequenced from an extensive sampling of burkholderia strains. this multigene approach results in a robust phylogeny, which places the bacterial endosymbiont ... | 2011 | 21378132 |
| analogues of peptide smap-29 with comparable antimicrobial potency and reduced cytotoxicity. | smap-29 (sheep myeloid antimicrobial peptide-29) is a peptide with potent antibacterial properties. however, it is also highly cytotoxic both to human red blood cells (hrbcs) and human embryonic kidney (hek) cells. in this study, some of the amino acids of smap-29 were changed in an attempt to reduce haemolytic activity whilst maintaining high antibacterial efficacy. these analogues, plus other analogues described in the literature with potent antimicrobial activity against gram-positive bacteri ... | 2011 | 21377841 |
| shape-memory properties and degradation behavior of multifunctional electro-spun scaffolds. | multifunctional polymer-based biomaterials, which combine degradability and shape-memory capability, are promising candidate materials for the realization of active self-anchoring implants. in this work we explored the shape-memory capability as well as the hydrolytic and enzymatic in vitro degradation behavior of electro-spun scaffolds prepared from a multiblock copolymer, containing hydrolytically degradable poly(p-dioxanone) (ppdo) and poly(e-caprolactone) (pcl) segments, which we have named ... | 2011 | 21374579 |
| predicting the success of fragment screening by x-ray crystallography. | fragment screening using x-ray crystallography is a method that can provide direct three-dimensional readouts of the structures of protein-small molecule complexes for lead development and fragment-based drug discovery. with current technology, an amenable crystal form can be screened crystallographically against a library of 1000-2000 fragments in 1-2 weeks. we have performed over a dozen crystallographic screening campaigns using our own compound collection called fragments of life™ (fol). whi ... | 2011 | 21371588 |
| phn and nag-like dioxygenases metabolize polycyclic aromatic hydrocarbons in burkholderia sp. c3. | burkholderia sp. c3 can transform polycyclic aromatic hydrocarbons (pahs), a class of ubiquitous pollutants, through multiple pathways, indicating existence of multiple dioxygenases (seo et al., in biodegradation 18:123-131, 2006a). both phn and nag-like genes in c3 were cloned and identified with the dna sequence alignment and the gene organization in the clusters. when cloned and expressed in escherichia coli, either the alpha- and beta-subunits of dioxygenase of the phn genes or the ferredoxi ... | 2011 | 21369832 |
| hyphae-colonizing burkholderia sp.-a new source of biological control agents against sheath blight disease (rhizoctonia solani ag1-ia) in rice. | sheath blight infection of rice by rhizoctonia solani kühn ag1-ia often results in serious yield losses in intensive rice cultivation. biological control agents (bcas) have previously been isolated but poor efficiency is often observed when applied under field conditions. this study compares a traditional dual-culture plate assay and a new water-surface microcosm assay for isolation of antagonistic soil bacteria. in the water-surface microcosm assay, floating pathogen mycelium is used as a sourc ... | 2011 | 21365233 |
| leveraging structure determination with fragment screening for infectious disease drug targets: mecp synthase from burkholderia pseudomallei. | as part of the seattle structural genomics center for infectious disease, we seek to enhance structural genomics with ligand-bound structure data which can serve as a blueprint for structure-based drug design. we have adapted fragment-based screening methods to our structural genomics pipeline to generate multiple ligand-bound structures of high priority drug targets from pathogenic organisms. in this study, we report fragment screening methods and structure determination results for 2c-methyl-d ... | 2011 | 21359640 |
| molecular mechanisms of chlorhexidine tolerance in burkholderia cenocepacia biofilms. | the high tolerance of biofilm-grown burkholderia cepacia complex bacteria against antimicrobial agents presents considerable problems for the treatment of infected cystic fibrosis patients and the implementation of infection control guidelines. in the present study, we analyzed the tolerance of planktonic and sessile burkholderia cenocepacia j2315 cultures and examined the transcriptional response of sessile cells to treatment with chlorhexidine. at low (0.0005%) and high (0.05%) concentrations, ... | 2011 | 21357299 |
| five novel acid-tolerant oligotrophic thiosulfate-metabolizing chemolithotrophic acid mine drainage strains affiliated with the genus burkholderia of betaproteobacteria and identification of two novel soxb gene homologues. | five acid-tolerant thiosulfate-metabolizing bacteria were isolated from acid mine drainage samples from garubathan, india. 16s rrna gene analysis revealed that the strains were affiliated with the genus burkholderia of the class of betaproteobacteria. comparative 16s rrna gene sequence analyses indicated that the strains designated as gah1 and gah2 produced a separate phylogenetic branch having burkholderia pyrrocinia atcc 51958(t) (96-98%) as the closest relative. strains gah4 and burkholderia ... | 2011 | 21349327 |
| acyldepsipeptide hdac inhibitor production induced in burkholderia thailandensis. | natural product gene clusters are often tightly regulated, resulting in gene cluster silencing in laboratory fermentation studies. the systematic overexpression of transcription factors (tfs) associated with biosynthetic gene clusters found in the genome of burkholderia thailandensis e264 identified a set of tfs that, when overexpressed, alter the secondary metabolome of this bacterium. the isolation and characterization of burkholdacs a and b, two new acyldepsitripeptide histone deacetylase inh ... | 2011 | 21348454 |
| involvement of the efflux pumps in chloramphenicol selected strains of burkholderia thailandensis: proteomic and mechanistic evidence. | burkholderia is a bacterial genus comprising several pathogenic species, including two species highly pathogenic for humans, b. pseudomallei and b. mallei. b. thailandensis is a weakly pathogenic species closely related to both b. pseudomallei and b. mallei. it is used as a study model. these bacteria are able to exhibit multiple resistance mechanisms towards various families of antibiotics. by sequentially plating b. thailandensis wild type strains on chloramphenicol we obtained several resista ... | 2011 | 21347382 |
| in vitro activity of fusidic acid (cem-102, sodium fusidate) against staphylococcus aureus isolates from cystic fibrosis patients and its effect on the activities of tobramycin and amikacin against pseudomonas aeruginosa and burkholderia cepacia. | we tested the mics of fusidic acid (cem-102) plus other agents against 40 methicillin-resistant staphylococcus aureus (mrsa) isolates from cystic fibrosis patients and the activities of fusidic acid with or without tobramycin or amikacin against pseudomonas aeruginosa, mrsa, and burkholderia cepacia isolates from cystic fibrosis patients in a 24-h time-kill study. fusidic acid was potent (mics, 0.125 to 0.5 µg/ml; a single 500-mg dose of fusidic acid at 8 h averaged 8 to 12. 5 µg/ml with 91 to 9 ... | 2011 | 21343445 |
| consistency of gene starts among burkholderia genomes. | evolutionary divergence in the position of the translational start site among orthologous genes can have significant functional impacts. divergence can alter the translation rate, degradation rate, subcellular location, and function of the encoded proteins. | 2011 | 21342528 |
| activity of a novel combination against multidrug-resistant nonfermenters: ceftazidime plus nxl104. | ampc overexpression appears to be a significant mechanism of ß-lactam resistance in pseudomonas aeruginosa and other nonfermenter gram-negatives. the article under review describes the in vitro activity of a novel combination ceftazidime plus nxl104, against ceftazidime-resistant pseudomonas aeruginosa, burkholderia cepacia complex and oxa carbapenemase-producing acinetobacter baumannii. the study results show that nxl104 overcomes the ceftazidime resistance engendered by ampc enzymes, even when ... | 2011 | 21342064 |
| quantitative proteomics (2-d dige) reveals molecular strategies employed by burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients under antimicrobial therapy. | chronic respiratory infections caused by burkholderia cenocepacia in patients with cystic fibrosis (cf) are characterized by low responsiveness to antibiotic therapy and, in general, to a more rapid decline of lung function. to get clues into the molecular mechanisms underlying the adaptive strategies employed to deal with the stressing conditions of the cf lung including antibiotic therapy, quantitative proteomics (2-d dige) was used to compare the expression programs of two clonal isolates ret ... | 2011 | 21337515 |