Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| tick salivary gland extract-activated transmission of borrelia afzelii spirochaetes. | saliva-activated transmission of borrelia afzelii canica, nato, du merle, mazie, baranton et postic, 1993 was demonstrated using salivary gland extract (sge) from ixodes ricinus (l., 1758) ticks and c3h mice. injection of borrelia spirochaetes together with sge increased the level of bacteraemia and accelerated the appearance of bacteria in the urinary bladder, compared with the injection of spirochaetes alone. more i. ricinus nymphs became infected when feeding on mice inoculated with b. afzeli ... | 2002 | 12194488 |
| horses and borrelia: immunoblot patterns with five borrelia burgdorferi sensu lato strains and sera from horses of various stud farms in austria and from the spanish riding school in vienna. | grazing animals are continuously exposed to tick bites. consequently, one may expect that horses will become infected with the various pathogens carried by ticks including borrelia burgdorferi sensu lato. whether horses may develop clinical disease due to this pathogen is controversially discussed. we were interested to learn about the infection of horses with borrelia burgdorferi sensu lato within one season by studying the dynamics of the humoral immune response in paired blood samples. the ma ... | 2002 | 12141766 |
| epitope mapping of the outer surface protein a (ospa) of the spirochete borrelia burgdorferi using a panel of monoclonal antibodies and lanthanide competition fluoroimmunoassay. | a panel of fourteen different monoclonal antibodies was used for detection and analysis of antigenic determinants located on the outer surface protein a (ospa) of the spirochete borrelia burgdorferi, which is a causative agent of tick-borne borreliosis (lyme disease). two main and several minor partially overlapping antigenic determinants have been found on the surface of the ospa protein of borrelia burgdorferi sensu stricto (strain 297) by lanthanide competition fluoroimmunoassay. one of the m ... | 2002 | 12126471 |
| three major lyme borrelia genospecies (borrelia burgdorferi sensu stricto, b. afzelii and b. garinii) identified by pcr in cerebrospinal fluid from patients with neuroborreliosis in sweden. | the lyme borrelia genospecies borrelia afzelii and b. garinii have previously been isolated using a culture method in swedish patients with lyme borreliosis (lb). there are reports suggesting that the genospecies distribution in human tissue specimens as determined by molecular methods is different from that obtained by culture. in the present study, we developed a nested pcr for detection of lyme borrelia-specific dna in cerebrospinal fluid from swedish patients with lb. the genospecies were su ... | 2002 | 12069016 |
| retrobulbar neuritis associated with borrelia afzelii infection. | to report retrobulbar neuritis caused by borrelia afzelii culturally proved from cerebrospinal fluid (csf). | 2002 | 12061028 |
| dna microarray assessment of putative borrelia burgdorferi lipoprotein genes. | a dna microarray containing fragments of 137 borrelia burgdorferi b31 putative lipoprotein genes was used to examine lyme disease spirochetes. dna from b. burgdorferi sensu stricto b31, 297, and n40; borrelia garinii ip90; and borrelia afzelii p/gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. these data show that a dna microarray can globally examine the genes encodin ... | 2002 | 12011030 |
| [lyme borreliosis--incidence of serum anti-myelin antibodies]. | the method of enzyme immunoassay (elisa) was used for detection of antibodies against the basic protein myelin (antimyelin antibodies) for a group of serum samples (n 36) with positive anti-borrelia immunoglobulins igg and igm (elisa-borrelia afzelii) and their immune complexes (elisa-peg). antimyelin antibodies (elisa-doxa kit-myelin basic protein antibodies) were assessed in 31% (n 11) of examined serum samples of patients with the working diagnosis of lyme borreliosis. statistical analysis (p ... | 2002 | 11987581 |
| [a patient with neuroborreliosis presenting gadolinium-enhanced mri lesions in bilateral facial nerves]. | we report a 33-year-old man with bilateral facial paralysis due to neuroborreliosis. about three weeks after rhinorrhea and fever lasting four days, he noticed fatigue in the legs and paresthesia in all four extremities. another week later, he developed paresthesia in his tongue and bilateral facial muscle weakness, and was admitted to our hospital. on admission, neurological examination revealed moderate bilateral facial muscle weakness, mild paresthesia in the tongue and four extremities, and ... | 2001 | 11968752 |
| [borrelia strains that cause erythema migrans in alsace, france]. | in europe, borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii are known as pathogens among the borrelia burgdorferi sensu lato group. since it is not yet known which borrelia are responsible for lyme borreliosis in france, the objective of this study was to identify the species of borrelia responsible for erythema migrans in the region of alsace, france. | 2002 | 11937923 |
| dynamics of dissemination and outer surface protein expression of different european borrelia burgdorferi sensu lato strains in artificially infected ixodes ricinus nymphs. | unfed ixodes ricinus nymphs were infected with eight different strains and clones of borrelia afzelii and b. garinii by capillary feeding. except one b. afzelii clone, all expressed ospc in culture. tick midguts and salivary glands were investigated at different time intervals for the presence of borreliae and for ospa and ospc phenotypes by immunofluorescence with simultaneous staining of ospa and ospc with monoclonal antibodies. both species were transmittable to i. ricinus. all ospc-expressin ... | 2002 | 11923373 |
| lyme disease and current aspects of immunization. | lyme disease is a tick-borne multisystem disease that affects primarily the skin, nervous system, heart and joints. at least three species of borrelia burgdorferi sensu lato, namely borrelia burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii, can cause the disease. this review will focus mainly on the pathophysiology of lyme arthritis, the long-term outcome of lyme disease, and the recently licensed vaccine against lyme disease. | 2002 | 11879534 |
| acrodermatitis chronica atrophicans and serologic confirmation of infection due to borrelia afzelii and/or borrelia garinii by immunoblot. | 1998 | 11864312 | |
| detection of borrelia afzelii, borrelia burgdorferi sensu stricto, borrelia garinii and group vs116 by pcr in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans. | objective: to evaluate the diagnostic performance of two polymerase chain reaction (pcr) procedures using skin biopsies of 20 erythema migrans (em) and 24 acrodermatitis chronica atrophicans (aca) patients. methods: one assay amplified a fragment of the outer surface protein (osp) a gene. the second method amplified the spacer region between the 5s and 23s rrna genes; hybridization of this fragment allowed identification of borrelia burgdorferi sensu lato species. results: among em patients, bot ... | 1997 | 11864084 |
| experimental infection of laboratory mice and rabbits with several isolates of borrelia burgdorferi sensu lato; comparison of antigens from different genospecies in serological measurement of immune responses. | infectivity of and immune responses to 28 finnish borrelia burgdorferi sensu lato isolates was studied in 3-4-week-old outbred nmri and inbred balb/c/hy laboratory mice; rabbits were also inoculated. twenty-one isolates were found to detectably infect mice. a variation among isolates in degree of infectivity was observed. higher infection rate and higher average elisa readings were recorded for intradermal than intraperitoneal inoculations. the results suggest differences between borrelia genosp ... | 2002 | 11848128 |
| distribution of clinically relevant borrelia genospecies in ticks assessed by a novel, single-run, real-time pcr. | a lightcycler-based pcr protocol was developed which targets the ospa gene for the identification and quantification of the different borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (hybprobe) and an internally labeled primer. the detection limit of the pcr was 1 to 10 spirochetes. a melting temperature determined from the melting curve of the amplified product immediately after thermal cycling allowed the differentiation of the ... | 2002 | 11773090 |
| [criteria for evaluation of immunoblots using borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto for diagnosis of lyme borreliosis]. | the immunoblot was prepared from genotypes borrelia afzelii (kc 90), borrelia garinii (m 192) and borrelia burgdorferi sensu stricto (b 31). sera of 63 patients with different forms of lyme borreliosis were examined and 40 healthy donors in the endemic area of the disease. in class igm in the group of patients significantly more frequently antibodies against ospc, p39, p41 b. afzelii, p39, p41, p66, p83 b. garinii and ospc1, ospa, b. burgdorferi sensu stricto were found. in class igg there were ... | 2001 | 11769176 |
| further characterization of complement regulator-acquiring surface proteins of borrelia burgdorferi. | the three genospecies borrelia burgdorferi, borrelia garinii, and borrelia afzelii, all causative agents of lyme disease, differ in their susceptibilities to human complement-mediated lysis. we recently reported that serum resistance of borrelias correlates largely with their ability to bind the human complement regulators fhl-1/reconectin and factor h. to date, two complement regulator-acquiring-proteins (crasp-1 and crasp-2) have been identified in serum-resistant b. afzelii isolates (p. kraic ... | 2001 | 11705962 |
| [use of indirect micromodification of immunoenzyme technique for serologic detection of ixodes tick-borne borreliosis (comparative analysis)]. | three hundred and seventy one sera sampled in different period since the onset of the disease from 214 patients with ixodes tick-borne borreliosdes (itbb) caused by borrelia afzelii and b. garinii in the perm region (russia) and 299 sera from 222 patients with other diseases were simultaneously tested for igm and igg antibodies by using immunofluorescence assay (ifa) with standard antigen from strain ip-21 b. afzelli by indirect micromodification of enzyme immunoassay (imelisa) on slides and rou ... | 2001 | 11702452 |
| lyme disease borrelia spp. in ticks and rodents from northwestern china. | in may 1999, field surveys of lyme disease spirochetes were conducted around the tianshan mountains in xinjiang uygur autonomous region in northwestern people's republic of china. ixodes persulcatus ticks were obtained in a tianchi lake valley with primary forest, while the tick fauna was poor in the semidesert or at higher altitudes in this region. species identities were confirmed by molecular analysis in which an internal transcribed spacer sequence was used. of 55 adult ticks, 22 (40%) were ... | 2001 | 11679340 |
| routine diagnosis of borrelia burgdorferi (sensu lato) infections using a real-time pcr assay. | to establish a one-tube fluorogenic real-time pcr assay for routine detection of borrelia burgdorferi (sensu lato) dna in various clinical specimens. | 2001 | 11678928 |
| borrelia burgdorferi sensu lato and ehrlichia spp. in ixodes ticks from southern norway. | we report the results of a study of the prevalence of ehrlichia and borrelia species in 341 questing ixodes ricinus ticks from two locations in southern norway. the prevalences of borrelia burgdorferi sensu lato and ehrlichia spp. were, respectively, 16 and 11.5% at site 1 and 17 and 6% at site 2. prevalence and species composition of borrelia and ehrlichia varied with location and date of collection. the dominant borrelia species at both sites was borrelia afzelii, followed by borrelia burgdorf ... | 2001 | 11574588 |
| isolation of borrelia afzelii from a dog. | 2001 | 11486768 | |
| evidence of ehrlichiosis agents found in ticks (acari: ixodidae) collected from migratory birds. | two ehrlichia pathogens were found in immature ixodes ricinus (l.) ticks collected from migratory passerine birds in the curonian spit area of the baltic region of russia (kaliningrad enclave). during the spring and fall of 2000, 1,606 passerine birds (eight species) were collected; 6.8% of them (110/1,606) were infested by ticks, and 51.8% (57/110) of tick clusters contained various human pathogenic microorganisms. human monocytic ehrlichiosis (hme) and human granulocytic ehrlichiosis (hge) age ... | 2001 | 11476325 |
| sensitive detection of borrelia burgdorferi sensu lato dna and differentiation of borrelia species by lightcycler pcr. | in order to differentiate species within the borrelia burgdorferi sensu lato complex, lightcyler pcr and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the reca gene, were performed. it was demonstrated that nested lightcycler pcr amplification of p66 is more sensitive in the detection of borrelia dna than amplification of the reca gene. b. burgdorferi sensu stricto could be differentiated from borrelia garinii and borrelia afzelii by melting ... | 2001 | 11427590 |
| visualization of borrelia burgdorferi sensu lato by fluorescence in situ hybridization (fish) on whole-body sections of ixodes ricinus ticks and gerbil skin biopsies. | the objective of this study was to visualize borreliae directly in whole-body sections of ixodes ricinus by fluorescence in situ hybridization (fish). borrelia afzelii mono-infected or borrelia burgdorferi sensu stricto (ss)/b. afzelii double-infected nymphs were fixed, embedded in cold polymerizing resin and sectioned. the same sample processing was applied to skin biopsies taken from a mongolian gerbil after an infectious tick-bite. fish was carried out using 16s-rrna-directed, fluorescence-la ... | 2001 | 11390674 |
| inhibition of borrelia burgdorferi-tick interactions in vivo by outer surface protein a antibody. | borrelia burgdorferi outer surface protein (osp) a is preferentially expressed by spirochetes in the ixodes scapularis gut and facilitates pathogen-vector adherence in vitro. here we examined b. burgdorferi-tick interactions in vivo by using abs directed against ospa from each of the three major b. burgdorferi sensu lato genospecies: b. burgdorferi sensu stricto, borrelia afzelii, and borrelia garinii. abs directed against b. burgdorferi sensu stricto (isolate n40) destroy the spirochete and can ... | 2001 | 11390491 |
| isolation and characterization of borrelia burgdorferi sensu lato strains in an area of italy where lyme borreliosis is endemic. | between 1993 and 1998, we isolated borrelia burgdorferi sensu lato from 55 of the 119 patients with clinically diagnosed lyme borreliosis who were admitted to "san martino" hospital in belluno, veneto, an adriatic region in northeastern italy where lyme borreliosis is endemic. upon hospitalization, all patients presented erythema migrans. isolates were typed using ribosomal dna pcr-restriction fragment length polymorphism (rflp) analysis of the rrfa-rrlb intergenic spacer. of the 41 isolates typ ... | 2001 | 11376066 |
| identification of ehrlichia spp. and borrelia burgdorferi in ixodes ticks in the baltic regions of russia. | the presence and distribution of ehrlichia spp. and borrelia burgdorferi sensu lato was demonstrated among ixodid ticks collected in the baltic regions of russia, where lyme borreliosis is endemic. a total of 3,426 ixodes ricinus and 1,267 ixodes persulcatus specimens were collected, and dark-field microscopy showed that 265 (11.5%) i. ricinus and 333 (26.3%) i. persulcatus ticks were positive. from these samples, 472 dark-field-positive and 159 dark-field-negative ticks were subjected to pcr an ... | 2001 | 11376063 |
| western blot analysis for diagnosis of lyme disease in acute facial palsy. | lyme borreliosis has been implicated in the pathogenesis of acute peripheral facial palsy (apfp). few studies, however, have used western blot analyses to confirm the serological diagnosis. | 2001 | 11359146 |
| complement evasion by borrelia burgdorferi: serum-resistant strains promote c3b inactivation. | the most characteristic features of the lyme disease pathogens, the borrelia burgdorferi sensu lato (s.l.) group, are their ability to invade tissues and to circumvent the immune defenses of the host for extended periods of time, despite elevated levels of borrelia-specific antibodies in serum and other body fluids. our aim in the present study was to determine whether b. burgdorferi is able to interfere with complement (c) at the level of c3 by accelerating c3b inactivation and thus to inhibit ... | 2001 | 11349031 |
| [first detection of ehrlichiosis detected serologically and with the polymerase chain reaction in patients with borreliosis in the czech republic]. | infectious complications caused by genus ehrlichia were confirmed when samples taken from patients with lyme borreliosis with non-characteristic development and those sent directly by physician who treated the patient were examined. laboratory diagnostics of ehrlichiosis was based on hematological examination of blood, on anamnestic and epidemiological data, on indirect immunofluorescence serology verified using western blot test with specific antigen and by determination of dna agents using pol ... | 2001 | 11347209 |
| [antibodies against borrelia afzelli in patients with an early stage of lyme disease]. | the aim of the study was evaluation of specific immune response against borrelia afzelii in patients with erythema migrans, as a sign of an early stage of lyme borreliosis. the study was performed in 42 patients, residents of podlasie province, who were initially evaluated by indirect immunofluorescence assay as a seropositive for borrelia burgdorferi sensu lato. antibodies against particular antigens of b. afzelii were demonstrated using a western-blot technique. evaluation of results showed th ... | 2001 | 11344697 |
| isolation of borrelia burgdorferi sensu lato from blood of patients with erythema migrans. | we assessed the isolation rate of borrelia burgdorferi sensu lato from blood in european patients with typical erythema migrans and evaluated the course and outcome of their illness. | 2001 | 11339477 |
| [culture of borrelia burgdorferi sensu lato from patients in the czech republic]. | spirochete of borrelia burgdorferi sensu lato were isolated in a modified bsk medium from 26 patients of 275 investigated suffering from early and late borreliosis. the isolates were specified by phenotype and genotype analysis using monoclonal antibodies, by immunochemical methods and by including species specific 16s rrna, ospa and ospc primers and probes in a polymerase chain reaction with hybridization. borrelia afzelii was found in 9 patients, borrelia garinii in 16 and b. burgdorferi sensu ... | 2001 | 11329731 |
| distinct levels of genetic diversity of borrelia burgdorferi are associated with different aspects of pathogenicity. | different species of pathogenic borrelia show different symptoms and tick vector specificity. even within regions where only one species is found, lyme disease progresses very differently from one patient to another. since borrelia shows very little recombination either within or between species, alleles of a gene can be used to mark clones. the ospc gene is highly variable within each species and can be used to define groups of related clones. it has been previously shown that only four out of ... | 2001 | 11316368 |
| cell-mediated immune response to high-passage borrelia spirochetes in c57bl/6 mice is strictly dependent on antigen specificity. | inbred c57bl/6 mice were challenged with high-passage borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto and tested for antigen specific t-cell response in vitro. sonicated preparations of washed spirochetes were potent cell activators, capable of stimulating polyclonal proliferation after 72h of culture while increasing the incubation time up to 120h provoked specific cell-mediated response. isolated murine spleocytes previously sensitized to b. burgdorferi sensu lato but ... | 2001 | 11297365 |
| characterization of lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern sweden. | southern sweden is an area of lyme borreliosis (lb) endemicity, with an incidence of 69 cases per 100,000 inhabitants. the most frequent clinical manifestations are erythema migrans (77%) and neuroborreliosis (16%). there was no record of human borrelia strains being isolated from patients in this region before the prospective study reported here. borrelia spirochetes were isolated from skin and cerebrospinal fluid (csf) from lb patients living in the region. a total of 39 strains were character ... | 2001 | 11283044 |
| isolation and polymerase chain reaction typing of borrelia afzelii from a skin lesion in a seronegative patient with generalized ulcerating bullous lichen sclerosus et atrophicus. | a 64-year-old woman presented with bullous and ulcerating lichen sclerosus et atrophicus (lsa) on the neck, trunk, genital and perigenital area and the extremities. histology of lesional skin showed the typical manifestations of lsa; in one of the biopsies spirochaetes were detected by silver staining. despite treatment with four courses of ceftriaxone with or without methylprednisone for up to 20 days, progression of lsa was only stopped for a maximum of 1 year. spirochaetes were isolated from ... | 2001 | 11251580 |
| rapid typing of borrelia burgdorferi sensu lato species in specimens from patients with different manifestations of lyme borreliosis. | to further investigate the pathogenic potential of different borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of lyme borreliosis were analyzed by pcr and reverse line blotting (rlb). in samples from lyme arthritis patients, b. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, borrelia garinii and borrelia afzelii, respectively, were exclusively detected. the results demonstrate that pcr-rl ... | 2001 | 11230440 |
| treatment resistant lyme arthritis caused by borrelia garinii. | lyme arthritis is caused in europe by three main pathogenic species of borrelia burgdorferi sensu lato: borrelia burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii. because few synovial samples have yet been analysed by species-specific dna amplification methods, further studies are needed to define the spectra of clinical manifestations associated with these different species. two cases of treatment resistant lyme arthritis are reported here, in which dna amplification of the fla ... | 2001 | 11171694 |
| tick salivary gland extract inhibits killing of borrelia afzelii spirochetes by mouse macrophages. | salivary gland extract (sge) from ixodes ricinus ticks inhibited the killing of borrelia afzelii spirochetes by murine macrophages. sge also reduced the production of two major defense molecules of phagocytes, superoxide and nitric oxide. it is likely that the suppression of macrophage microbicidal mechanisms contributes to the inhibitory effect of tick saliva on the killing of b. afzelii spirochetes, thus facilitating the transmission of this important pathogen. | 2001 | 11119556 |
| the complement regulator factor h binds to the surface protein ospe of borrelia burgdorferi. | spirochete bacteria of the borrelia burgdorferi sensu lato complex cause lyme borreliosis. the three pathogenic subspecies borrelia garinii, borrelia afzelii, and borrelia burgdorferi sensu stricto differ in their disease profiles and susceptibility to complement lysis. we investigated whether complement resistance of borreliae could be due to acquisition of the main soluble inhibitors of the alternative complement pathway, factor h and the factor h-like protein 1. when exposed to nonimmune edta ... | 2001 | 11113124 |
| relative incompetence of european rabbits for lyme disease spirochaetes. | to determine whether rabbits may serve as reservoir hosts for lyme disease spirochaetes in europe, we compared their competence as hosts for borrelia afzelii, one of the most prevalent european spirochaetal variants, with that of the mongolian jird. to infect rabbits or jirds, at least 3 nymphal or adult ixodes ricinus ticks infected with spirochaetes fed to repletion on each animal. whereas jirds readily acquired tick-borne lyme disease spirochaetes and subsequently infected vector ticks, rabbi ... | 2000 | 11085249 |
| borreliacidal activity of early lyme disease sera against complement-resistant borrelia afzelii fem1 wild-type and an ospc-lacking fem1 variant. | sera obtained from 14 lyme borreliosis patients at early stages (stages i and ii) of the disease were examined for their borreliacidal properties against borrelia afzelii isolate fem1 by use of a growth inhibition assay. five of 14 immune sera exhibited borreliacidal activity against isolate fem1. heat-inactivated immune sera failed to kill the spirochaetes. immunoblotting experiments with outer-membrane preparations showed that ospc and 11 additional proteins of 14.0, 16.0, 17.7, 19.3, 21.7, 27 ... | 2000 | 11023189 |
| lyme disease. | lyme disease (ld) is the most common tick-borne disease in the us. the overall trend has been an average annual increase in cases since surveillance was initiated by the centers for disease control and prevention in 1982. to date, 10 different borrelia species have been described within the borrelia burgdorferi sensu lato complex, although only borrelia burgdorferi sensu strico, borrelia garinii, and borrelia afzelii have been associated with human disease. ixodes ticks often carry more than one ... | 2000 | 10910185 |
| diagnosis of lyme disease in two cows by the detection of borrelia burgdorferi dna. | two cows from different herds in a district of switzerland known to harbour ixodid ticks had erythematous lesions on the hairless skin of the udder, were in poor general condition with a poor appetite and decreased milk production, and had a stiff gait and swollen joints. borrelia burgdorferi sensu strictu dna was detected in samples of synovial fluid and milk from one of the cows and borrelia afzelii dna was detected in synovial fluid from the other by means of a real-time pcr. | 2000 | 10887997 |
| isolation of borrelia afzelii from overwintering culex pipiens biotype molestus mosquitoes. | during the years 1995-1996, a total of 1,743 overwintering culex pipiens biotype molestus female mosquitoes were tested for the presence of spirochetes in several localities in south moravia, czech republic.the spirochetes were observed in 5% of the mosquitoes investigated. one of the five isolated strains of spirochetes (br-84) was identified as borrelia afzelii. the potential role of mosquitoes in the ecology and epidemiology of lyme disease (ld) borreliae should be further investigated. | 1999 | 10885843 |
| rapid differentiation of borrelia garinii from borrelia afzelii and borrelia burgdorferi sensu stricto by lightcycler fluorescence melting curve analysis of a pcr product of the reca gene. | to differentiate the borrelia burgdorferi sensu lato genospecies, lightcycler real-time pcr was used for the fluorescence (sybr green i) melting curve analysis of borrelial reca gene pcr products. the specific melting temperature analyzed is a function of the gc/at ratio, length, and nucleotide sequence of the amplified product. a total of 32 dna samples were tested. of them three were isolated from b. burgdorferi reference strains and 16 were isolated from b. burgdorferi strains cultured from i ... | 2000 | 10878080 |
| antibodies against specific proteins of and immobilizing activity against three strains of borrelia burgdorferi sensu lato can be found in symptomatic but not in infected asymptomatic dogs. | in an area where lyme disease is endemic in the netherlands all dogs had positive titers by whole-cell enzyme-linked immunosorbent assay and appeared to be naturally infected by borrelia burgdorferi sensu lato. to compare the antibody responses of symptomatic dogs and asymptomatic controls, we performed western blots and in vitro immobilization assays to study antibody-dependent bactericidal activity. strains from three different genospecies were employed as the antigen source: b. burgdorferi st ... | 2000 | 10878052 |
| the cell-mediated immune response to borrelia afzelii, garinii and burgdorferi in c57bl/6 mice is dependent on antigen specificity. | inbred c57bl/6 mice were challenged with borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto and tested for antigen-specific t-cell response in vitro. the sonicated preparations of in vitro grown spirochetes were capable of stimulating polyclonal proliferation and specific cell-mediated response, depending on duration of the cell culture. murine splenocytes previously sensitized to b. burgdorferi sensu lato (s.l. ), but not those from control mice, could be induced for anti ... | 2000 | 10865176 |
| colorimetric in vitro susceptibility testing of penicillins, cephalosporins, macrolides, streptogramins, tetracyclines, and aminoglycosides against borrelia burgdorferi isolates. | the spectrum of antibiotic susceptibility of borrelia burgdorferi has been only partially defined. in the present study the effectiveness of 12 antimicrobials, belonging to six different antibiotic classes have been tested against borrelia burgdorferi s.s. (n=3), borrelia garinii (n=3), borrelia afzelii (n=3), borrelia valaisiana (n=1), and borrelia bissettii (n=1) isolates. these isolates were analysed by a new standardised colorimetric minimal inhibitory concentration (mic) method based upon c ... | 2000 | 10856671 |
| occurrence of borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii in the ixodes ricinus ticks from eastern slovakia. | a total of 2816 unfed adults nymphs of ixodes ricinus ticks were collected from vegetation in kosice (eastern slovakia) from 1994 to 1997. prevalence of borrelia burgdorferi s. l. in i. ricinus ticks, detected by dark field microscopy, varied and depended upon the year and the habitat of the collected ticks. the lowest prevalence was observed in 1994 (4.8%). during 1995 it increased to 17.2% and during the next two years decreased to 15.5% and 14.2%. the rate of infection varied from 2.1 to 23.3 ... | 2000 | 10845258 |
| detection and genotyping of borrelia burgdorferi sensu lato by polymerase chain reaction. | to isolate and genotype borrelia burgdorferi genospecies in serum samples of croatian patients with erythema migrans. | 2000 | 10810167 |
| an improved recombinant igg immunoblot for serodiagnosis of lyme borreliosis. | we have previously described the use of the following recombinant antigens for serodiagnostic immunoblots: p83/100, p39, ospc and p41 (flagellin) internal fragment [wilske et al. (1993) med microbiol immunol 182:255-270; rossler et al. (1997) j clin microbiol 35:2752-2758]. in our currently used immunoblot p83/100 is derived from strain pko (borrelia afzelii), p39 (bmpa) and ospc from strains pka2 (b. hurgdorferi sensu stricto), pko and pbi (b. garinii), respectively; the p41 (flagellin) interna ... | 1999 | 10776844 |
| distribution of twelve linear extrachromosomal dnas in natural isolates of lyme disease spirochetes. | we have analyzed a panel of independent north american isolates of the lyme disease agent spirochete, borrelia burgdorferi (sensu stricto), for the presence of linear plasmids with sequence similarities to the 12 linear plasmids present in the b. burgdorferi type strain, isolate b31. the frequency of similarities to probes from each of the 12 b31 plasmids varied from 13 to 100% in the strain panel examined, and these similarities usually reside on plasmids similar in size to the cognate b31 plas ... | 2000 | 10762248 |
| characterization of borrelia burgdorferi strains isolated from korea by 16s rdna sequence analysis and pcr-rflp analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons. | haenam strains of borrelia burgdorferi, which had been isolated from ixodes granulatus and apodemus agrarius in haenam, korea, were characterized by pcr-rflp analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons and by sequence analysis of the 16s rrna gene (rdna). the msel and dral restriction patterns of the 5s-23s intergenic spacer amplicons of haenam strains differed from those of other b. burgdorferi sensu lato strains. furthermore, in the phylogenetic tree based on the 16s rdna sequen ... | 2000 | 10758897 |
| new colorimetric microdilution method for in vitro susceptibility testing of borrelia burgdorferi against antimicrobial substances. | a newly developed colorimetric microdilution method was used to analyze the activity of 12 antimicrobial agents against nine borrelia burgdorferi isolates, including all three genospecies pathogenic for humans. in addition, in vitro antimicrobial resistance patterns of borrelia valaisiana and borrelia bissettii tick isolates were investigated. the applied test system is based upon color changes that occur in the presence of phenol red and result from the accumulation of nonvolatile acid produced ... | 2000 | 10706176 |
| identification of borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii in ixodes ricinus ticks from southern bohemia using monoclonal antibodies. | the prevalence of borrelia burgdorferi sensu lato (s.l.) in 761 ixodes ricinus ticks collected in different localities of southern bohemia was investigated. the minimum infection rate of nymphs, males and females as determined by dark-field microscopy was 4.8% in 1997. the total b. burgdorferi prevalence determined in ticks in 1998 was 11.04%. a significant local variability of the prevalence of b. burgdorferi was not found. nine isolates were identified at a genospecies level by immunoblotting ... | 2000 | 10705611 |
| [single-step polymerase chain reaction in the detection of borrelia burgdorferi and the genome species in the ixodes ricinus tick]. | the objective of the work to introduce screening pcr into the diagnosis of borrelia burgdorferi sensu lato in the vector selection of the most suitable primer, derived from chromosomal dna and detection of different genome species. the sensitivity of primers, described in the literature (ld, 16s, wk, 5s-23s) was tested by different amounts of dna strains of borrelias. the most sensitive primer--ld was used for detection of borrelias in the vector. ticks were collected in municipal parks from 199 ... | 1999 | 10658345 |
| transcriptional analysis of a superoxide dismutase gene of borrelia burgdorferi. | a single superoxide dismutase (sod) gene was identified in borrelia burgdorferi strains, borrelia afzelii ple and borrelia garinii pbi. recombinant enzymatic activity was detected only when sod expression was controlled by the lacz promoter in the cloning vector. northern blot analysis with sod- or seca-specific probes identified a common 3.7-kb transcript. reverse transcriptase-pcr analysis confirmed that seca and sod constitute a single transcriptional unit in b. burgdorferi. a transcriptional ... | 2000 | 10650199 |
| positive serology for lyme disease borrelias in primary cutaneous b-cell lymphoma: a study in 22 patients; is it a fortuitous finding? | the historical association of acrodermatitis chronica atrophicans (aca), now known to be a late manifestation of lyme disease caused by borrelia afzelii, with cutaneous lymphoma, and several small series of pcbcl with positive lyme disease borrelial serology initiated a study of this association. material and methods in the last 9 years, 30 patients with pcbcl have been observed and followed, 22 of them were tested for borrelial serology. the control group consisted of 85 patients with nhl (10 c ... | 1999 | 10641031 |
| determination of members of a borrelia afzelii-related group isolated from ixodes nipponensis in korea as borrelia valaisiana. | the 16s rrna sequences of the korean borrelia strains 5mt and 9mt, isolated from ixodes nipponensis, showed identities of 99.0-99.1% to that of b. afzelii. the strains were tentatively classified as belonging to the b. afzelii-related group. in this study, korean isolates, including these strains, were characterized further and compared with recently described new species. these strains generated a rflp pattern that has not been found previously in rflp analysis of the 5s-23s rrna intergenic spa ... | 1999 | 10555320 |
| molecular typing of borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. | borrelia burgdorferi sensu lato, the spirochete that causes human lyme borreliosis (lb), is a genetically and phenotypically divergent species. in the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the lb-related spirochetes and their potential association with distinct clinical syndromes. these methods include serotyping, multilocus enzyme electrophoresis, dna-dna reassociation analysis, rrna gene restri ... | 1999 | 10515907 |
| incidence of borrelia garinii and borrelia afzelii in ixodes ricinus ticks in an urban environment, prague, czech republic, between 1995 and 1998. | the presence of borrelia burgdorferi sensu lato and its individual genospecies was determined using a single-step polymerase chain reaction in a total of 779 ixodes ricinus ticks collected from an urban park in prague, czech republic. the incidence of borrelia burgdorferi sensu lato was 9.2% in 1995, 3.4% in 1996, 4.5% in 1997 and 2.8% in 1998. the borrelia garinii to borrelia afzelii ratio was 1.4:1 and it did not differ significantly throughout the study period. borrelia burgdorferi sensu stri ... | 1999 | 10482032 |
| evidence for frequent ospc gene transfer between borrelia valaisiana sp. nov. and other lyme disease spirochetes. | molecular polymorphism of the ospc gene has been reported in borrelia burgdorferi sensu stricto, borrelia garinii and borrelia afzelii, the spirochetes causing human lyme borreliosis. to assess the genetic relationship between ospc genes from the recently described borrelia valaisiana sp. nov. and other b. burgdorferi sensu lato species, the ospc genes from eight b. valaisiana isolates were amplified by pcr, cloned and sequenced. the ospc genes of three b. valaisiana isolates were identical, but ... | 1999 | 10474195 |
| persistence of borrelia garinii and borrelia afzelii in patients with lyme arthritis. | we repeatedly detected dna of borrelia garinii or b. afzelii and borrelia-like structures in the blood, joint fluid or in the synovium of 10 patients with lyme arthritis by means of the polymerase chain reaction and immunoelectron microscopy at 2-4-month intervals in the course of two years. all samples were analyzed using primers which amplified the 16s rrna gene sequence of borrelia burgdorferi sensu lato and nucleotide sequences for the ospa gene. no cross hybridization occurred with dna from ... | 1999 | 10467661 |
| phenotypic and genetic characterization of a novel borrelia burgdorferi sensu lato isolate from a patient with lyme borreliosis. | borrelia burgdorferi sensu lato a14s was cultured from a skin biopsy specimen of a patient with erythema migrans in the netherlands. this isolate had a unique dna fingerprint pattern compared to 135 other b. burgdorferi sensu lato isolates. in this study, the isolate a14s was further characterized by protein analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and reactivity with various monoclonal antibodies. in addition, the 16s rrna, ospa, and ospc genes, as well ... | 1999 | 10449497 |
| salivary gland extract from ixodes ricinus ticks inhibits production of interferon-gamma by the upregulation of interleukin-10. | tick saliva has been shown to modulate host immunity by a so far unknown mechanism. we have demonstrated an inverted effect of salivary gland extract (sge), derived from partially fed ixodes ricinus females, on the production of two cytokines, interferon (ifn)-gamma and interleukin (il)-10, in vitro. while sge markedly suppressed the elaboration of ifn-gamma by mouse splenocytes stimulated with lipopolysaccharide (lps), the production of il-10 was increased in comparison with sge-untreated cultu ... | 1999 | 10417669 |
| comparative analysis and immunological characterization of the borrelia bdr protein family. | multiple circular and linear plasmids of lyme disease and relapsing fever borrelia spirochetes carry genes for members of the bdr (borrelia direct repeat) protein family. to define their common and divergent attributes, we first comprehensively compared the known homologs. bdr proteins with predicted sizes ranging from 10.7 to 30. 6 kda formed five homology groups, based on variable numbers of short direct repeats in a central domain and diverse n- and c-terminal domains. in a further characteri ... | 1999 | 10377099 |
| validity of interpretation criteria for standardized western blots (immunoblots) for serodiagnosis of lyme borreliosis based on sera collected throughout europe. | western blotting (wb; immunoblotting) is a widely used tool for the serodiagnosis of lyme borreliosis (lb), but so far, no generally accepted criteria for performance and interpretation have been established in europe. the current study was preceeded by a detailed analysis of wb with whole-cell lysates of three species of borrelia burgdorferi sensu lato (u. hauser, g. lehnert, r. lobentanzer, and b. wilske, j. clin. microbiol. 35:1433-1444, 1997). in that study, interpretation criteria for a pos ... | 1999 | 10364592 |
| osp17, a novel immunodominant outer surface protein of borrelia afzelii: recombinant expression in escherichia coli and its use as a diagnostic antigen for serodiagnosis of lyme borreliosis. | western blot analyses of the human humoral response of patients with lyme borreliosis have shown that a 17-kda protein is an immunodominant protein in late disease. immune electron microscopy with a monoclonal antibody against this protein revealed that the 17-kda protein is abundantly expressed on the surface of borrelia afzelii strain pko. therefore, the protein has been renamed outer surface protein (osp) 17. recombinant osp 17 of strain pko was expressed in escherichia coli and purified by c ... | 1999 | 10363678 |
| density of deer in relation to the prevalence of borrelia burgdorferi s.l. in ixodes ricinus nymphs in rambouillet forest, france. | the rambouillet forest, a lyme disease-endemic area near paris, france, was surveyed from september 1994 to october 1995 to determine the risk periods and zones for humans. firstly, during the period of ixodes ricinus activity, abundance of nymphs is greater in spring than in autumn. secondly, we observed significant variation in nymphal abundance between zones according to the density of cervids. the polymerase chain reaction (pcr) was used to detect dna of borrelia burgdorferi sensu lato in 46 ... | 1999 | 10356769 |
| sexual transmission of borrelia garinii by male ixodes persulcatus ticks (acari, ixodidae). | we investigated the transmission of borrelia garinii and borrelia afzelii between male and female ixodes persulcatus ticks. for this purpose the infection rate of partners from tick couples was determined by polymerase chain reaction and reverse line blot. in couples, where the male tick was infected with b. garinii, four out of nine female partners carried b. garinii. in eight couples, male ticks had a dual infection of b. afzelii and b. garinii and three female partners were infected by borrel ... | 1999 | 10356767 |
| isolation of borrelia afzelii from circumscribed scleroderma. | a 45-year-old man presented with circumscribed scleroderma (cs) on the extremities. histology of lesional skin showed the typical manifestations of scleroderma including a perivascular and interstitial infiltrate of lymphocytes and plasma cells; in one of the biopsies spirochaetes could be detected. despite treatment with penicillin, progression of cs was observed and spirochaetes were isolated from skin cultures obtained from active scleroderma lesions. these spirochaetes were identified as bor ... | 1999 | 10354035 |
| diagnostic detection and direct genotyping of borrelia burgdor feri regular by polymerase chain reaction in cerebrospinal fluid in lyme neuroborreliosis. | background: a dna target imbalance in favor of the plasmid-borne outer surface protein a (ospa) versus chromosomal genes has been thought to explain the relatively high diagnostic sensitivity of an ospa-based polymerase chain reaction (pcr) on joint fluid from patients with lyme arthritis. the aim of this study was to evaluate the diagnostic sensitivity of the ospa-based pcr on cerebrospinal fluid (csf) samples from patients with lyme neuroborreliosis and to perform dna sequence analysis on the ... | 1998 | 10089270 |
| transmission of borrelia afzelii from apodemus mice and clethrionomys voles to ixodes ricinus ticks: differential transmission pattern and overwintering maintenance. | this study deals with the ecology of lyme borreliosis in europe. the relationships between borrelia burgdorferi sensu lato spirochetes, clethrionomys and apodemus rodent reservoirs and the ixodes ricinus tick vector were investigated during 16 consecutive months in an enzootic area in switzerland. cultivation of ear skin biopsies was used to isolate spirochetes from c. glareolus, a. sylvaticus, a. flavicollis and glis glis. borrelia infection was more frequently observed in clethrionomys than in ... | 1999 | 10070659 |
| [borrelia burgdorferi, taxonomy, pathogenicity and spread]. | borrelia burgdorferi sensu lato is a bacterial complex of at least 10 species, most of which are non pathogenic for humans. non pathogenic species seem to be more vector-specific, their distribution being restricted to that of this vector. in opposition, the three species pathogenic for humans, borrelia burgdorferi sensu stricto (b.b.s.s.), borrelia garinii (b.g.), and borrelia afzelii (b.a.), are transmitted by several species of ticks more diversified in their host choice. each of these three ... | 1998 | 9921400 |
| comparison of culture-confirmed erythema migrans caused by borrelia burgdorferi sensu stricto in new york state and by borrelia afzelii in slovenia. | the clinical manifestations of lyme borreliosis in north america and europe seem to differ, but a systematic comparison has never been done. | 1999 | 9890847 |
| specificity of infection-induced immunity among borrelia burgdorferi sensu lato species. | the specificity of infection-induced immunity in mice infected with cultured or host-adapted borrelia burgdorferi sensu lato, the agent of lyme disease, was examined. sera obtained from mice following infection with high and low doses of cultured b. burgdorferi sensu stricto, transplantation of infected tissue (host-adapted spirochetes), or tick-borne inoculation all showed protective activity in passive immunization assays. infection and disease were similar in mice infected with cultured spiro ... | 1999 | 9864193 |
| genetic heterogeneity of borrelia burgdorferi sensu lato in ixodes ricinus ticks collected in belgium. | borrelia burgdorferi sensu lato (s.l.), the etiological agent of lyme disease, is transmitted by the bite of ixodes ricinus. four hundred eighty-nine ticks, collected in four locations of a region of southern belgium where lyme disease is endemic, were examined for the presence of the spirochete. in a pcr test with primers that recognize a chromosomal gene of all strains, 23% of the ticks were found to be infected. the species b. burgdorferi s.l. comprises at least three pathogenic genomospecies ... | 1998 | 9774592 |
| clinical features of lyme borreliosis in the middle urals and distribution of borrelia burgdorferi sensu lato species in local ixodes persulcatus ticks. | from 1991 to 1993, we investigated the clinical features of lyme borreliosis (lb) in 864 patients from the sverdlovsk region (population 4.5 million) in the middle urals. ixodes persulcatus ticks were collected in the vicinity of yekaterinburg to determine the presence of borrelia burgdorferi sensu lato species. from 1991 to 1993, the number of patients with lb increased from 91 to 320 and 453, respectively. nearly all lb patients (97%) recalled a tick bite and the first signs of lb developed be ... | 1998 | 9728411 |
| augmented intrathecal secretion of interferon-gamma in response to borrelia garinii in neuroborreliosis. | the lyme disease agent borrelia garinii has been suggested to be neurotrop, preferentially affecting the nervous system. we compared the secretion of interferon-gamma in response to outer surface proteins from borrelia garinii and borrelia afzelii in 10 patients with neuroborreliosis. in cerebrospinal fluid, stimulation with borrelia garinii revealed higher numbers of interferon-gamma-secreting cells in all patients, whereas in blood, only five displayed higher numbers. this further strengthens ... | 1998 | 9726840 |
| vector competence of ixodes scapularis and ixodes ricinus (acari: ixodidae) for three genospecies of borrelia burgdorferi. | the vector competence of 2 tick species, ixodes ricinus (l.) and ixodes scapularis say, was determined and compared for 3 genospecies of borrelia burgdorferi. the 3 genospecies of b. burgdorferi used in the following experiments were borrelia burgdorferi sensu stricto (b-31 and b-31.d1 clone), borrelia afzelii (strain pgau. c3), and borrelia garinii (strain vs286 and vsbp). spirochetes from all 5 strains were inoculated intradermally into outbred mice; larval ticks of both species were subsequen ... | 1998 | 9701928 |
| failure of ixodes ticks to inherit borrelia afzelii infection. | to define conditions promoting inherited infection by lyme disease spirochetes in ixodes ticks, we variously infected ticks with borrelia afzelii and examined their progenies by dark-field microscopy, immunofluorescence, pcr, and serial passage. no episode of inherited infection was evident, regardless of instar or gender infected or frequency of exposure. we suggest that these spirochetes rarely, if ever, are inherited by vector ticks. | 1998 | 9687480 |
| lyme disease borrelia species in northeastern china resemble those isolated from far eastern russia and japan. | fifty-nine borrelia burgdorferi sensu lato culture isolates collected from northeastern china were characterized by 5s-23s rrna intergenic spacer restriction fragment length polymorphism (rflp) analysis and reactivity with monoclonal antibodies (mabs). among 59 culture isolates, 30 (50.8%) were borrelia garinii and 17 (28.8%) were borrelia afzelii, 2 were mixtures composed of b. garinii with rflp pattern b and b. garinii with pattern c, and 9 were mixtures composed of b. garinii and b. afzelii. ... | 1998 | 9647853 |
| expression of outer surface proteins a and c of borrelia afzelii in ixodes ricinus ticks and in the skin of mice. | several studies have described changes in the expression of proteins, especially of ospa and ospc, of b. burgdorferi sensu stricto during tick feeding. in this study, the expression of ospa and ospc of b. afzelii in unfed and feeding i. ricinus nymphs and in the subsequent adults was followed by means of the immunofluorescence test. spirochaetes expressing ospa and ospc were observed in 70% and 80%, respectively of the unfed nymphs. in feeding and in fully engorged ticks, spirochaetes expressed ... | 1998 | 9638876 |
| rate of infection of ixodes ricinus ticks with borrelia burgdorferi sensu stricto, borrelia garinii, borrelia afzelii and group vs116 in an endemic focus of lyme disease in italy. | a study to evaluate the natural rate of infection of ixodes ricinus with borrelia burgdorferi sensu lato was carried out in an endemic focus of lyme disease in the trieste area in northern italy. two-hundred and twenty-seven ticks collected in ten different stations were tested individually for the presence of the spirochetes using polymerase chain reaction techniques able to identify both borrelia burgdorferi sensu lato and the four genospecies (borrelia burgdorferi sensu stricto, borrelia gari ... | 1998 | 9629972 |
| borrelia burgdorferi stimulates the production of interleukin-10 in peripheral blood mononuclear cells from uninfected humans and rhesus monkeys. | heat-killed borrelia burgdorferi spirochetes stimulate in vitro production of interleukin-10 (il-10) at both mrna and protein levels in peripheral blood mononuclear cells (pbmc) of uninfected rhesus monkeys. a concomitant down-modulation of il-2 gene transcription was observed. neither il-4 nor gamma interferon gene expression was ostensibly affected by b. burgdorferi spirochetes. these phenomena were observed regardless of whether the stimulating spirochetes belonged to the b. burgdorferi sensu ... | 1998 | 9596735 |
| experimental infection of cattle with several borrelia burgdorferi sensu lato strains; immunological heterogeneity of strains as revealed in serological tests. | twenty-three experimental cattle, mainly calves, were each inoculated 1-3 times with one of ten finnish borrelia burgdorferi sensu lato strains. all three genospecies were represented. borreliae were administered mainly by both intravenous (about 10(6) to 10(9) spirochaetes) and intradermal (10(4)) routes, and on six occasions subcutaneously (10(3)) only. for infectivity control and comparison purposes mice and rabbits were inoculated simultaneously. immune responses in cattle were monitored bot ... | 1998 | 9595625 |
| ixodes ricinus strains in europe. | an analysis of cuticular hydrocarbons (ch) of unfed adult ixodes ricinus ticks collected throughout europe showed that there are 10 distinct i. ricinus groups. studies on the seasonal and annual consistency of ch composition and possible effects of host and environmental factors suggested that chs may be used as a genuine genetic marker for i. ricinus. preliminary studies compared the vector competence of ticks from three of the most separated i. ricinus groups and the results suggested that the ... | 1998 | 9580422 |
| enzyme-linked immunosorbent assay using recombinant ospc and the internal 14-kda flagellin fragment for serodiagnosis of early lyme disease. | the outer surface protein c (ospc) and the internal 14-kda flagellin fragment of strain geho of borrelia burgdorferi sensu stricto were expressed as recombinant proteins in escherichia coli and were purified for use in an immunoglobulin m (igm) enzyme-linked immunosorbent assay (ospc-14-kda antigen elisa). no hint at disturbing protein-protein interferences, which might influence the availability of immunoreactive epitopes, was found when the recombinant antigens were combined in the elisa. the ... | 1998 | 9542898 |
| tick-borne borrelioses pathogen identification in ixodes ticks (acarina, ixodidae) collected in st. petersburg and kaliningrad baltic regions of russia. | two isolated baltic seashore populations of ixodes ticks were studied as vectors of different borrelia genospecies in russia by using darkfield microscopy and modified polymerase chain reaction (pcr). in the kalinigrad region (kurish spit, forests near the settlements of lesnoye and rybachy), 788 ixodes ricinus (l.) adults and nymphs were collected by flagging and studied by darkfield microscopy during 1995-1996. there were 88 darkfield microscopy positive specimens (11.2%) of which 69 were also ... | 1998 | 9538573 |
| antigenic and genetic structure of borrelia burgdorferi. | lyme borreliosis is a disease caused by the spirochaetes borrelia burgdorferi, borrelia afzelii and borrelia garinii and it is transmitted by ticks. most of the proteins (outer surface proteins, flagellar proteins and other uncertain location proteins) have a strong antigenic variability. osp a protein genetic and serological studies facilitated the differentiation of seven serotypes strongly correlated with the known genospecies. the genetic structure of these spirochaetes included a large line ... | 1997 | 9523423 |
| isolation of the spirochaete borrelia afzelii from the mosquito aedes vexans in the czech republic. | during the years 1993-1995, a total of 3580 culicine mosquitoes of six species were collected in south moravia, czech republic, and examined by dark-field microscopy for the presence of borreliae. females of aedes cantans, ae. sticticus, ae. vexans, culex pipiens and cx pipiens biotype molestus (but not ae. geniculatus or culiseta annulata) harboured spirochaetes, the frequencies ranging from 0.7% to 7.8%. one isolate (br-53) from ae. vexans was identified as borrelia afzelii genospecies. the po ... | 1998 | 9513946 |
| molecular typing of borrelia burgdorferi sensu lato by randomly amplified polymorphic dna fingerprinting analysis. | to study whether pathogenic clusters of borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic dna (rapd) fingerprinting with four arbitrary primers. the rapd patterns were reproducible up to the 95% similarity level as shown in duplicate experiments. in these experiments the purified dnas prepare ... | 1998 | 9508310 |
| analysis of the organization of multicopy linear- and circular-plasmid-carried open reading frames in borrelia burgdorferi sensu lato isolates. | plasmid cp8.3 of borrelia afzelii ip21 carries several open reading frames (orfs) and a 184-bp inverted repeat (ir) element. it has been speculated that this plasmid may encode factors involved in virulence or infectivity. in this report, we have characterized the distribution, molecular variability, and organization of orfs 1, 2, and 4 and the ir elements among isolates of the borrelia burgdorferi sensu lato complex. orfs 1 and 2 are contained within a segment of cp8.3 that is bordered by the i ... | 1998 | 9488408 |
| impact of strain heterogeneity on lyme disease serology in europe: comparison of enzyme-linked immunosorbent assays using different species of borrelia burgdorferi sensu lato. | for the standardization of serological tests for lyme borreliosis (lb) in europe, the influence of the heterogeneity of borrelia burgdorferi sensu lato must be assessed in detail. for this study four immunoglobulin m (igm) and igg enzyme-linked immunosorbent assays (elisas) with octyl-beta-d-glucopyranoside extracts of strains pko (borrelia afzelii), pbi (borrelia garinii), and pka2 and b31 (both b. burgdorferi sensu stricto) were compared. strains pko, pbi, and pka2 at the passages used for ant ... | 1998 | 9466753 |
| [the isolation and results of the study of the protein structure of spirochetes isolated from i. ricinus ticks in byelarus]. | four hundred and eighty ixodes ricinus (278 female and 202 male) samples collected in the natural biotopes of 13 administrative districts in southwest byelarus were studied via dsk-h medium (sigma) inoculation. twenty-four spirochetes isolates (3, 2, 1, and 18 in the brest, gomel, mogilev, and grodno regions, respectively) were obtained on the territory of byelarus. after adapted to the medium, most isolates (as many as 7 x 10(6)-5 x 10(7) microbial cells per ml) in the stationary phase. all the ... | 1997 | 9445994 |
| species-specific plasmid sequences for pcr identification of the three species of borrelia burgdorferi sensu lato involved in lyme disease. | species-specific sequences were shown to be carried by plasmids of the three main species of borrelia burgdorferi sensu lato involved in lyme disease. libraries of the 16-, 33-, and 25-kb plasmids of b. burgdorferi sensu stricto, borrelia garinii, and borrelia afzelii, respectively, were then built and used to isolate species-specific sequences. after sequencing of the cloned inserts, three sets of primers were designed. they were shown to determine species-specific pcr amplification products. t ... | 1998 | 9431963 |
| sudeck's atrophy in lyme borreliosis. | a patient with disseminated lyme borreliosis is reported. the patient suffered from erythema migrans and radicular pain. serologic tests routinely performed (ift, elisa, western blots with different strains and borrelia-ltt) were negative. however, borrelia burgdorferi (genotype borrelia afzelii) was cultivated from a skin biopsy. western blot with the patient's isolate and sera showed strong reactivity only with the 60 kda protein. in spite of immediate diagnosis and intravenous antibiotic trea ... | 1997 | 9427058 |