Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| assessment of gastroenteric viruses from wastewater directly discharged into uruguay river, uruguay. | the aim of this study was to assess the viral contamination of group a rotavirus (rva), norovirus (nov), and human astrovirus (hastv) in sewage directly discharged into uruguay river and to characterize rva genotypes circulating in uruguay. for this purpose, sewage samples (n = 96) were collected biweekly from march 2011 to february 2012 in four uruguayan cities: bella unión, salto, paysandú, and fray bentos. each sample was concentrated by ultracentrifugation method. qualitative and quantitativ ... | 2014 | 24777819 |
| occurrence and characterization of rotavirus a in broilers, layers, and broiler breeders from brazilian poultry farms. | rotaviruses are a major cause of diarrhea in humans and animals, including several mammalian and avian species. using different pcr protocols, we report the occurrence of rotavirus a in 21 (53.84%; 21/39) from 39 fecal pool samples of broilers, layers, and broiler breeders from brazilian avian farms. we typed the g5, g8, g11, g19, and p[31] genotypes. | 2014 | 24758129 |
| the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain and mortality in 4 danish pig herds. | the study evaluated the effect of new neonatal porcine diarrhoea syndrome (nnpds) on average daily gain (adg) and mortality and described the clinical manifestations in four herds suffering from the syndrome. nnpds is a diarrhoeic syndrome affecting piglets within the first week of life, which is not caused by enterotoxigenic escherichia coli (etec), clostridium perfringens (c. perfringens) type a/c, clostridium difficile (c. difficile), rotavirus a, coronavirus, cystoisospora suis, strongyloide ... | 2014 | 24755093 |
| distinct evolutionary origins of g12p[8] and g12p[9] group a rotavirus strains circulating in brazil. | g12 group a rotavirus (rva) are currently recognized as a globally emerging genotype and have been described in combination with several p-types. in brazil, g12 rva strains have been described in the southern (2003) and northern (2008-2010) regions, in combination with the p[9] and p[6] genotype, respectively. to date, few complete genomes of g12 rva strains have been described (none from brazilian strains), considering g12p[9] genotype just one strain, rva/human-tc/tha/t152/1998/g12p[9], has th ... | 2014 | 24747965 |
| molecular analysis of non structural rotavirus group a enterotoxin gene of bovine origin from india. | the rotavirus enterotoxin nsp4 (nonstructural protein 4), plays a pivotal role in viral morphogenesis as well as pathogenesis. in this study, the nsp4 gene of rotavirus group a (rva) isolates of bovine origin isolated in several states of india from 2008 to 2011 were characterized. the complete open reading frame of 23 rva strains were sequenced and analyzed phylogenetically. genotype e1 was detected for the first time in bovines from india, in addition to the more common bovine genotype e2. seq ... | 2014 | 24747605 |
| [prognosis of rotavirus infection in children]. | the aim of the research was to develop the method for predicting the course of rotavirus gastroenteritis in children.under the supervision were 3607 children aged from 9 days to 5 years with the diagnosis of "acute gastroenteritis" and "acute gastroenterocolitis". the diagnosis of rotavirus infection was on the basis of a set of clinical, epidemiological data and the results of para-clinical and bacteriological studies and data of detection of rotavirus antigen strain. genotyping of rotavirus gr ... | 2014 | 24743127 |
| the first caprine rotavirus detected in argentina displays genomic features resembling virus strains infecting members of the bovidae and camelidae. | rotavirus group a (rva) is a major cause of diarrhea in humans and young animals including small ruminants. the purpose of this study was to identify rva in dairy goat kids, and to characterize the complete genomic constellation and genetic relatedness with other rva strains. four out of twenty fecal samples from diarrheic and non-diarrheic goat kids were positive for rva by elisa. a representative sample was selected for further genome analyses. the rva strain rva/goat-wt/arg/0040/2011/g8p[1] d ... | 2014 | 24742949 |
| identification of novel bovine group a rotavirus g15p[14] strain from epizootic diarrhea of adult cows by de novo sequencing using a next-generation sequencer. | there are few reports describing diarrhea of adult cattle caused by group a rotaviruses. here, we report the identification of a novel bovine group a rotavirus from diarrhea of adult cows. a group a rotavirus was detected from an epizootic outbreak of diarrhea in adult cows with a decrease in milk production in japan in 2013. the comprehensive genomic analyses from fecal samples by viral metagenomics using a next-generation sequencer revealed that it had an unreported genotype combination g15p[1 ... | 2014 | 24725447 |
| [serological survey of antibodies against viral diseases of public health interest in llamas (lama glama) from jujuy province, argentina]. | llama population from argentina is mainly concentrated in the andean puna, jujuy. llamas represent an important economic resource for the andean communities. the aim of this study was to investigate the prevalence of antibodies against viral antigens associated to viral diseases of economic impact (neonatal diarrhea, reproductive and respiratory syndromes). a total of 349 serum samples from adult llamas were analyzed. the obtained antibody prevalence was 100 % for rotavirus a and 70 % for bovine ... | 2014 | 24721276 |
| a two-tube multiplex reverse transcription pcr assay for simultaneous detection of viral and bacterial pathogens of infectious diarrhea. | diarrhea caused by viral and bacterial infections is a major health problem in developing countries. the purpose of this study is to develop a two-tube multiplex pcr assay using automatic electrophoresis for simultaneous detection of 13 diarrhea-causative viruses or bacteria, with an intended application in provincial centers for diseases control and prevention, china. the assay was designed to detect rotavirus a, norovirus genogroups gi and gii, human astrovirus, enteric adenoviruses, and human ... | 2014 | 24711998 |
| rotavirus infection among sudanese children younger than 5 years of age: a cross sectional hospital-based study. | in sudan, rotavirus has been one of the important causative agents of diarrhea among children. rotavirus a is well known as the leading cause of diarrhea in young children worldwide. it was estimated to account for 41% of hospitalized cases of acute gastroenteritis among children in sub-saharan africa. this study aimed to determine the prevalence and the common clinical presentations of rotavirus a infection among sudanese children with gastroenteritis seeking management in hospitals. | 2013 | 24711878 |
| full-length genomic analysis of porcine rotavirus strains isolated from pigs with diarrhea in northern italy. | group a rotaviruses (rva) cause acute dehydrating diarrhea in young of man and many animal species, including pigs. swine rva has an important economic impact on the farming industry, and pigs represent a potential reservoir for zoonotic transmission of rva to humans. to investigate the genetic diversity of porcine rva strains in italy and identify their possible zoonotic characteristics, 25 rva-positive feces were collected from diarrheic pigs in northern italy, in 2009-2010; all viral strains ... | 2014 | 24704759 |
| evaluation of the rapid test for human rotavirus a in thai children with acute gastroenteritis. | rapid tests are widely used to detect rotavirus a; however, pediatricians are concerned whether the rapid test can still accurately detect the virus. therefore, this study evaluated the performance of the rotavirus rapid test by comparing it to the one-step rt-pcr method. | 2014 | 24697131 |
| virucidal effect of commercially available disinfectants on equine group a rotavirus. | although many disinfectants are commercially available in the veterinary field, information on the virucidal effects of disinfectants against equine group a rotavirus (rva) is limited. we evaluated the performance of commercially available disinfectants against equine rva. chlorine- and iodine-based disinfectants showed virucidal effects, but these were reduced by the presence of organic matter. glutaraldehyde had a virucidal effect regardless of the presence of organic matter, but the effect wa ... | 2014 | 24681569 |
| sequence and structural analyses of nsp4 proteins from human group a rotavirus strains detected in tunisia. | the nsp4 protein of group a rotavirus (rva) has been recognized as a viral enterotoxin and plays important roles in viral pathogenesis and morphogenesis. domains involved in structural and functional interactions have been proposed mainly based on the simian sa11 strain. | 2014 | 24679587 |
| full-genome sequence of a rare human g3p[9] rotavirus strain. | this is a report of the complete genomic sequence of a rare rotavirus group a g3-p[9]-i2-r2-c2-m2-a3-n2-t1-e2-h3 strain designated rva/human-wt/usa/12us1134/2012/g3p[9]. | 2014 | 24675848 |
| predominance of rotavirus g8 genotype in hospitalized children with acute gastroenteritis in yasuj, iran. | rotavirus infection is the most common cause of gastroenteritis among children worldwide. in this study, we were evaluated the occurrence of rotavirus infection and genotypes causing gastroenteritis among children under 5 years in yasuj, iran. | 2014 | 24668711 |
| evaluation of a multiplex pcr assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients. | we evaluated a multiplex pcr assay, the seeplex diarrhoea ace detection, that simultaneously detects 15 enteric pathogens, including salmonella spp., shigella spp., vibrio spp., toxin b producer clostridium difficile, campylobacter spp., clostridium perfringens, yersinia enterocolitica, aeromonas spp., escherichia coli o157:h7, verocytotoxin-producing escherichia coli, adenovirus, group a rotavirus, norovirus gi and gii, and astrovirus. we compared this assay with clinical methods routinely used ... | 2014 | 24656922 |
| fluorescence in situ hybridization investigation of potentially pathogenic bacteria involved in neonatal porcine diarrhea. | neonatal diarrhea is a multifactorial condition commonly present on pig farms and leads to economic losses due to increased morbidity and mortality of piglets. immature immune system and lack of fully established microbiota at birth predispose neonatal piglets to infection with enteric pathogens. the microorganisms that for decades have been associated with enteritis and diarrhea in suckling piglets are: rotavirus a, coronavirus, enterotoxigenic escherichia coli (etec), clostridium perfringens t ... | 2014 | 24628856 |
| diarrhoea-related hospitalizations in children before and after implementation of monovalent rotavirus vaccination in mexico. | to assess, by socioeconomic setting, the effect of nationwide vaccination against species a rotavirus (rva) on childhood diarrhoea-related hospitalizations in mexico. | 2014 | 24623905 |
| surveillance of pathogens causing gastroenteritis and characterization of norovirus and sapovirus strains in shenzhen, china, during 2011. | viral gastroenteritis is one of the most common diseases in humans, and it is primarily caused by rotaviruses (rvs), astroviruses (astvs), adenoviruses (advs), noroviruses (novs), and sapoviruses (savs). in this study, we determined the distribution of viral gastroenteritis and human calicivirus (hucvs) in acute gastroenteritis patients in shenzhen, china, during 2011. real-time rt-pcr was used to detect norovirus (nov), group a rotavirus (rv), adenovirus (adv), and astrovirus (astv). from a tot ... | 2014 | 24610551 |
| complete genomic sequence analyses of the first group a giraffe rotavirus reveals close evolutionary relationship with rotaviruses infecting other members of the artiodactyla. | group a rotaviruses (rva) have been established as significant contributory agents of acute gastroenteritis in young children and many animal species. in 2008, we described the first rva strain detected in a giraffe calf (rva/giraffe-wt/irl/girrv/2008/g10p[11]), presenting with acute diarrhoea. molecular characterisation of the vp7 and vp4 genes revealed the bovine-like genotypes g10 and p[11], respectively. to further investigate the origin of this giraffe rva strain, the 9 remaining gene segme ... | 2014 | 24582453 |
| utility of specific biomarkers to assess safety of swine manure for biofertilizing purposes. | swine production is an important economic activity in brazil, and there is interest in the development of clean production mechanisms to support sustainable agro-industrial activities. the biomass derived from swine manure has good potential to be used as a biofertilizer due to its high nutrient concentration. however, the land application of manure should be based on safety parameters such as the presence of pathogens that can potentially infect animals and people. this study was designed to as ... | 2014 | 24565860 |
| [research progress of real-time quantitative pcr method for group a rotavirus detection]. | group a rotavirus is one of the most significant etiological agents which causes acute gastroenteritis among infants and young children worldwide. so far, several method which includes electron microscopy (em), enzyme immunoassay (eia), reverse transcription-polymerase chain reaction (rt-pcr)and real-time quantitative pcr has been established for the detection of rotavirus. compared with other methods, real-time quantitative pcr have advantages in specificity, sensitivity, genotyping and quantit ... | 2013 | 24520772 |
| [isolation and characterization of rotavirus from bat]. | bats are considered as important animal reservoirs for many pathogenic viruses to humans. the viral metagenomic analysis was performed to study gut and lung tissues of 30 insectivorous bats collected in yunnan province and 26 reads were noted to group a rotavirus (rva). further rt-pcr screening on bat samples and in vitro viral isolation on cell cultures confirmed the presence of a novel rva, named as rva/bat-tc/myas33/2013/g3p[10], in one of 30 stoliczka's trident bats. the vp7 gene of this str ... | 2013 | 24520769 |
| first molecular detection of group a rotavirus in urban and hospital sewage systems by nested-rt pcr in shiraz, iran. | group a rotaviruses are the most significant cause of acute gastroenteritis in children worldwide. rotaviruses are shed in high numbers and dispersed widely throughout bodies of water in the environment. this represents a significant health hazard for humans, mainly due to the stability of the viruses during wastewater treatment processes. this study was conducted to evaluate the prevalence of rotaviruses, to determine g genotypes of circulating rotaviruses and to assess the efficiency of rotavi ... | 2013 | 24499551 |
| simultaneous detection of five enteric viruses associated with gastroenteritis by use of a pcr assay: a single real-time multiplex reaction and its clinical application. | we developed a highly sensitive reverse transcription and multiplex real-time pcr (rtpcr) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups i and ii; sapovirus genogroups i, ii, iv, and v; human rotavirus a; adenovirus serotypes 40 and 41; and human astrovirus. in comparison to monoplex rtpcr assays, the sensitivities and specificities of the multiplex rtpcr ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clini ... | 2014 | 24478418 |
| genetic diversity of rotavirus strains circulating in environmental water and bivalve shellfish in thailand. | rotavirus is a common cause of acute diarrhea in young children worldwide. this study investigated the prevalence and molecular characterization of rotavirus in environmental water and oyster samples in thailand. a total of 114 water samples and 110 oyster samples were collected and tested for group a rotavirus using rt-nested pcr. rotavirus genotype was identified by phylogenetic analysis of the vp7 genetic sequences. group a rotavirus was detected in 21 water samples (18.4%) and six oyster sam ... | 2014 | 24469269 |
| circulation of group a rotaviruses among neonates of human, cow and pig: study from assam, a north eastern state of india. | rotavirus (rv) infections are worldwide in distribution causing high morbidity and mortality in human and animal neonates. human settlements in close proximity of animals aids for genetic re-assortment of the virus by interspecies transmission and consequent emergence of new viral antigenic strain. therefore, the present study was designed to explore rv incidence in a single approach from human and animal neonates sharing similar environment. altogether, 200 diarrheal samples from children (50), ... | 2013 | 24426283 |
| group a rotaviruses in children with gastroenteritis in a canadian pediatric hospital: the prevaccine era. | a publicly funded, group a rotavirus (rva) vaccination program was implemented in quebec in november 2011. | 2013 | 24421793 |
| diversity of rotavirus strains causing diarrhea in <5 years old chinese children: a systematic review. | we conducted a systematic review of the diversity and fluctuation of group a rotavirus strains circulating in china. | 2014 | 24416267 |
| detection and genetic characterization of human enteric viruses in oyster-associated gastroenteritis outbreaks between 2001 and 2012 in osaka city, japan. | enteric viruses are an important cause of viral food-borne disease. shellfish, especially oysters, are well recognized as a source of food-borne diseases, and oyster-associated gastroenteritis outbreaks have on occasion become international occurrences. in this study, 286 fecal specimens from 88 oyster-associated gastroenteritis outbreaks were examined for the presence of 10 human enteric viruses using antigenic or genetic detection methods in order to determine the prevalence of these infection ... | 2014 | 24415518 |
| molecular analysis of group a rotaviruses detected in adults and adolescents with severe acute gastroenteritis in italy in 2012. | hospital-based surveillance of acute gastroenteritis caused by rotavirus has produced ample knowledge on the infection in children, whereas little is known on rotavirus infection among adults. the italian surveillance program rotanet-italia collected 1,595 samples from patients admitted to hospital with gastroenteritis in 2012. all patients presented with vomiting, diarrhea, fever, and/or abdominal pain. forty-two samples obtained by the rotanet-italia (2.6%) were from adolescents or adults (10- ... | 2014 | 24415411 |
| genetic diversity of group a rotavirus in swine in canada. | group a rotaviruses (rva) in pigs have been poorly investigated in canada. in a continued effort to fill this gap, ten finisher swine farms in quebec, canada, were sampled over a nine-month period. the presence of rva was detected in healthy pigs on all farms investigated during the entire sampling period. the genotypes detected included g2, g5, g9 and g11; p[6], p[7], p[13], p[27] and p[34]; and i5 and i14. the predominant types were g2, p[13] and i5, which is different from previous global rep ... | 2014 | 24402632 |
| group a rotavirus and bacterial agents associated with diarrhoea-induced hospitalisations in children below 5 years of age in jammu. | out of 210 faecal samples collected from children below 5 years attending different hospitals in jammu and exhibiting clinical signs of diarrhoea, 41.9% samples were found positive for group a rotavirus by rna-page. escherichia coli isolated in the study belonged to nine serogroups, out of which o69 was most frequent, being present in 12.38% samples. e. coli serogroups well recognised as enteropathogens viz. o69, o20 and o153 were present in 27.6% samples. other bacterial pathogens associated wi ... | 2014 | 24399390 |
| molecular epidemiologic trends of diarrhea-causing virus infection from clinical specimens in cheonan, korea, in 2010-2012. | the enteric viruses have high health significance in humans, ranging from poliomyelitis, hepatitis, and gastroenteritis to innocuous infections, and the human enteric viral infection is the one of the most common diseases during early childhood. this study was conducted from july 2010 to october 2012. | 2014 | 24375926 |
| detection of picobirnavirus and rotavirus in diarrhoeic faecal samples of cattle and buffalo calves in mumbai metropolis, western india. | in this study 113 diarrhoeic faecal samples obtained from buffalo (n = 68) and cattle (n = 45) calves under 1 years of age were analysed in order to determine the presence of rotavirus infection and the frequency of picobirnavirus excretion. eleven (9.73%) samples positive for group a rotavirus were identified through rna-polyacrylamide gel electrophoresis (rna-page), while 4 (3.53%) samples showed a bisegmented genome with a typical picobirnavirus pattern. this is the first report of picobirnav ... | 2013 | 24362776 |
| prevalence of rotavirus genotypes in south korea in 1989-2009: implications for a nationwide rotavirus vaccine program. | the epidemiology of human group a rotavirus was analyzed by examining genotypic data acquired from 1989 to 2009 in south korea. this information was derived from all the available published articles on rotavirus studies in south korea, retrieved from both the pubmed and koreamed databases. four common g types (g1, g2, g3, and g4) and three common p types (p[8], p[4], and p[6]) accounted for approximately 93% and 99% of the rotavirus reports, respectively. the g9 type was frequently detected afte ... | 2013 | 24348658 |
| epidemiology of rotavirus diarrhea and diversity of rotavirus strains among children less than 5 years of age with acute gastroenteritis in mauritius: june 2008 to december 2010. | rotavirus is an important etiologic agent of diarrhea worldwide and ongoing surveillance is essential to monitor strain diversity. to describe the epidemiology of rotavirus disease and circulating rotavirus strains in mauritius, surveillance for rotavirus diarrhea was conducted at 2 regional hospitals from june 2008 through december 2010. | 2014 | 24343614 |
| hospital-based surveillance for rotavirus gastroenteritis in children younger than 5 years of age in ethiopia: 2007-2012. | rotavirus surveillance was initiated in ethiopia to estimate the burden of rotavirus gastroenteritis in children <5 years of age, to generate data to assist the policy-making process for new vaccine introduction and to monitor impact of vaccination on disease burden after introduction. | 2014 | 24343610 |
| real-time rt-pcr assays to differentiate wild-type group a rotavirus strains from rotarix(®) and rotateq(®) vaccine strains in stool samples. | group a rotaviruses (rva) are the leading cause of severe diarrhea in young children worldwide. two live-attenuated rva vaccines, rotarix(®) and rotateq(®) are recommended by world health organization (who) for routine immunization of all infants. rotarix(®) and rotateq(®) vaccines have substantially reduced rva associated mortality but occasionally have been associated with acute gastroenteritis (age) cases identified in vaccinees and their contacts. high-throughput assays are needed to monitor ... | 2014 | 24342877 |
| improved detection of gastrointestinal pathogens using generalised sample processing and amplification panels. | we aimed to streamline the diagnosis of gastrointestinal disease by producing multiplexed real time polymerase chain reaction (pcr) panels employing universal sample processing for dna and rna containing pathogens. a total of 487 stored, previously characterised stool samples comprising bacterial, viral, protozoan and clostridium difficile positive samples were tested using four multiplexed real time pcr panels. a further 81 pre-selected clinical samples from a teaching hospital were included to ... | 2014 | 24300711 |
| complete genotype constellation of human rotavirus group a circulating in thailand, 2008-2011. | this study has identified diverse and re-assorted group a rotavirus (rva) strains by sequence and phylogenetic analysis of the 11 genomic segments. the 22 cases investigated in this study were collected from children with diarrhea between 2008 and 2011. the rva genomic constellations identified in this study were identified as g1-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1 22.7% (5/22); g2-p[4]-i2-r2-c2-m2-a2-n2-t2-e2-h2 27.3% (6/22); g3-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1 18.2% (4/22); g3-p[9]-i3-r3-c3-m3-a3-n ... | 2014 | 24296010 |
| a fut2 gene common polymorphism determines resistance to rotavirus a of the p[8] genotype. | attachment to carbohydrates of the histo-blood group type of several human rotavirus strains (rva) has recently been described. synthesis of these ligands requires a functional fut2 enzyme, suggesting that fut2 null homozygote (ie, nonsecretor) individuals may not be recognized by most human rva strains. whereas such individuals represent 20% of the control population, this retrospective study determined that none of 51 patients infected by p[8] rotavirus strains were nonsecretors. the lack of α ... | 2014 | 24277741 |
| g8p[6] rotaviruses isolated from amerindian children in mato grosso do sul, brazil, during 2009: close relationship of the g and p genes with those of bovine and bat strains. | during the 2009 national group a rotavirus (rva) surveillance, five unusual strains of the human g8p[6] genotype were detected in brazilian indian children with acute gastroenteritis. the aim of this study was to carry out sequence analysis of the two outer capsid proteins (vp4 and vp7) and the inner capsid protein (vp6) of the g8p[6] strains detected in order to provide further information on the genetic relationship between human and animal rva. a total of 68 stool samples, collected in mato g ... | 2014 | 24259191 |
| complete genome analysis of a rare human g3p[9] rotavirus posing as an au-1 like strain. | we performed phylogenetic and sequence analysis by basic local alignment search tool (blast) of a complete human rotavirus (hrv) genome isolated from a hospitalized child with acute gastroenteritis in thailand. | 2013 | 24255863 |
| molecular characterization of human g8p[4] rotavirus strains in italy: proposal of a more complete subclassification of the g8 genotype in three major lineages. | in 2011, two children with acute rotavirus gastroenteritis were hospitalized in sardinia, italy. two rva strains with g8p[4] genotype were detected in their stools, and were named ss56/2011 and ss65/2011. the aim of the study was to characterize these two rare strains, collected within a national rva gastroenteritis surveillance program. eight of the 11 rva genes were sequenced and phylogenetic analysis performed. vp7 amino acid sequence was also analyzed. sequencing of genes encoding the vp4, v ... | 2014 | 24252348 |
| detection and characterization of potentially zoonotic viruses in faeces of pigs at slaughter in germany. | pigs can harbour a variety of viruses in their gastrointestinal tract. some of them are closely related to human viruses and are therefore suspected to have a zoonotic potential. only little is known about the presence of those viruses in pigs at slaughter. however, by contamination of meat with zoonotic viruses during the slaughtering process, food-borne transmission to humans may be possible. here we analyzed 120 faecal samples of pigs at slaughter from 3 different geographical regions of germ ... | 2014 | 24247020 |
| phylogenetic analysis of the rotavirus genotypes originated from children < 5 years of age in 16 cities in south korea, between 2000 and 2004. | the purpose of this study was to examine the diversity of the g and p types of human rotavirus strains isolated in south korea during 2000 to 2004. | 2012 | 24159485 |
| detection of rotavirus in children with acute gastroenteritis in porto velho, rondonia, brazil. | rotavirus group a (rva) infection was ascertained in 591 fecal samples from children 0 to 6 years old with acute gastroenteritis. the vaccination status was also verified in all 591 subjects, with 302 (51.0 % ± 4.0 %) participants fully vaccinated against rotavirus. forty-two of the vaccinated children (13.9 % ± 3.9 %) tested positive for rva infection. of the 289 unvaccinated children (49.0 % ± 4.0 %), 61 (21.1 % ± 4.7 %) had stools positive for rva. this study suggests that the proportion of a ... | 2014 | 24158345 |
| epidemiological and genetic analysis of human group c rotaviruses isolated from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012. | group c rotavirus (gcrv) infection has been described in several parts of the world, predominantly as sporadic cases of acute gastroenteritis. little is known about the yearly changes in the gcrv strains from diarrheal outbreaks. stool samples collected from outbreaks of acute gastroenteritis in yokohama, japan, between 2006 and 2012 that were negative for norovirus, sapovirus, and group a rotavirus, were screened for gcrv using a reverse passive hemagglutination method. the gcrv strains were ch ... | 2014 | 24154950 |
| diversity of rotavirus strains circulating in northern brazil after introduction of a rotavirus vaccine: high prevalence of g3p[6] genotype. | rotavirus a (rva) is the most common cause of severe acute gastroenteritis in infants and young children worldwide, causing 453,000 deaths annually. in brazil, the most frequent genotype identified was g1 during almost three decades in the pre-vaccination period; however, after anti-rotavirus vaccine introduction, there was a predominance of g2 genotype. the aim of this study was to determine the g and p genotypes of rotaviruses isolated from children under 5 years of age with acute gastroenteri ... | 2014 | 24136444 |
| added value of multiplex luminex gastrointestinal pathogen panel (xtag® gpp) testing in the diagnosis of infectious gastroenteritis. | the luminex gastrointestinal pathogen panel (xtag(®) gpp) detects in one assay the most common gastroenteritis-causing pathogens and toxins, namely adenovirus 40/41, norovirus genogroup (ng) i/ii, rotavirus a, clostridium difficile toxin a/b, campylobacter sp., escherichia coli o157, enterotoxigenic e. coli heat-labile enterotoxin/heat-stable enterotoxin, salmonella sp., shiga-toxin producing e. coli, shiga-like toxin (stx)1/2, shigella sp., vibrio cholerae, yersinia enterocolitica, cryptosporid ... | 2014 | 24131399 |
| detection and molecular characterization of diarrhea causing viruses in single and mixed infections in children: a comparative study between bangladesh and turkey. | the incidence and mortality caused by diarrhea differ among countries. the prevalence of different enteric viruses, their molecular characteristics, and infections with multiple viruses might affect the disease incidence and mortality caused by diarrhea. the objective of this study was to determine the distribution and molecular characteristics of enteric viruses in children with diarrhea in turkey and bangladesh. a total of 288 stool samples that were negative for group a rotavirus were collect ... | 2014 | 24105741 |
| rotavirus in adults, brazil, 2004-2011: g2p[4] dominance and potential impact on vaccination. | the aim of this study was to monitor rotavirus (rv) infections in adults >18 years with acute gastroenteritis during 2004-2011 national brazilian rv surveillance. in addition, to characterize the rv group a (rva) strains in order to gain insight into the supposed vaccine selective pressure imposed to brazilian children population. | 2014 | 24076114 |
| simultaneous detection of waterborne viruses by multiplex real-time pcr. | norovirus, rotavirus group a, the hepatitis a virus, and coxsackievirus are all common causes of gastroenteritis. conventional diagnoses of these causative agents are based on antigen detection and electron microscopy. to improve the diagnostic potential for viral gastroenteritis, internally controlled multiplex real-time polymerase chain reaction (pcr) methods have been recently developed. in this study, individual real-time pcrs were developed and optimized for specific detections of norovirus ... | 2013 | 24037661 |
| characterization of a novel g3p[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses. | bats are considered important animal reservoirs for many viruses pathogenic to humans. an approach based on viral metagenomics was used to study gut specimens from 78 insectivorous bats in yunnan province, china. seventy-four reads were found to be related to group a rotavirus (rva). further reverse transcription-pcr screening and viral isolation on cell cultures confirmed the presence of a novel rva strain, named rva/bat-tc/mslh14/2012/g3p[3], in 1 (6%) of 16 lesser horseshoe bats. full genomic ... | 2013 | 24027312 |
| characterization of the nsp4 gene of group a human rotavirus g1p[8] strains circulating in sapporo, japan from 1987 to 2000. | the genetic diversity of the nsp4 gene of rotavirus g1p[8] strains obtained in sapporo was analyzed, japan from 1987 to 2000. sixty-four strains, which were distributed across the whole study period, were included. all g1p[8] nsp4 genes detected in this study belonged to genotype e1, which divided into at least three lineages. the sapporo rotavirus g1p[8] isolates were found in each lineage. the mean estimated substitution rate was 1.40 × 10(-3) nucleotide substitutions per site per year, which ... | 2014 | 24026858 |
| identification of circulating porcine-human reassortant g4p[6] rotavirus from children with acute diarrhea in china by whole genome analyses. | p[6] group a rotavirus (rva) strains identified in four stool specimens collected from children with acute diarrhea in guangxi province, southern china in 2010, with unknown g type were further analyzed by full genomic analysis. it was revealed by whole genome sequencing that 11 genomic cognate gene segments of these p[6] rva strains shared almost 100% nucleotide identities and all exhibited an identical g4-p[6]-i1-r1-c1-m1-a8-n1-t1-e1-h1 genotype constellation. phylogenetic analyses of vp7, vp1 ... | 2013 | 24012957 |
| etiological study of enteric viruses and the genetic diversity of norovirus, sapovirus, adenovirus, and astrovirus in children with diarrhea in chongqing, china. | enteric viruses are a major cause of diarrhea in children, especially those <5 years old. identifying the viral agents is critical to the development of effective preventive measures. this study aimed to determine the prevalence of common enteric viruses in children <5 years old presented with diarrhea to the children's hospital of chongqing medical university. | 2013 | 24004442 |
| detection of rotavirus from hospitalized diarrheic children in uttar pradesh, india. | in the present study 220 stool samples collected from diarrheic children admitted to different hospitals and nursing homes of uttar pradesh and uttarakhand were screened for rotavirus. of 220 diarrheic samples screened 46 samples were found to be positive for rotavirus by rna page. all the isolates exhibited 4-2-3-2 migration pattern suggesting group a rotavirus. both long and short electropherotypes were prevalent in these regions. six different electropherotypes were detected in this study per ... | 2012 | 23997341 |
| global distribution of group a rotavirus strains in horses: a systematic review. | group a rotavirus (rva) is a major cause of diarrhea and diarrhea-related mortality in foals in parts of the world. in addition to careful horse farm management, vaccination is the only known alternative to reduce the rva associated disease burden on horse farms. the precise evaluation of vaccine effectiveness against circulating strains needs enhanced surveillance of equine rvas in areas where vaccine is already available or vaccine introduction is anticipated. therefore, we undertook the overv ... | 2013 | 23994380 |
| molecular characterization of the porcine group a rotavirus nsp2 and nsp5/6 genes from são paulo state, brazil, in 2011/12. | rotaviruses are responsible for the acute diarrhea in various mammalian and avian species. the nonstructural proteins nsp2 and nsp5 are involved in the rotavirus replication and the formation of viroplasm, cytoplasmic inclusion bodies within which new viral particles morphogenesis and viral rna replication occur. there are few studies on the genetic diversity of those proteins; thus this study aims at characterizing the diversity of rotavirus based on nsp2 and nsp5 genes in rotaviruses circulati ... | 2013 | 23970830 |
| viral and bacterial infections associated with camel (camelus dromedarius) calf diarrhea in north province, saudi arabia. | diarrhea and deaths in new-born camel calves were noticed by veterinary investigators and pastoralist in saudi arabia to be very high. hence, it is thought to be necessary to investigate this problem from the virological and bacteriological point of view. the role of pathogenic bacteria and viruses in six different towns of north province (al-assafia, arar, domat aljandal, hail, skaka and khoa) in saudi arabia was studied. survey was conducted in diarrheic camel calves aged 12 months or younger. ... | 2011 | 23961160 |
| evidence of natural transmission of group a rotavirus between domestic pigs and wild boars (sus scrofa) in japan. | group a rotaviruses (rvas) are a major cause of acute dehydrating diarrhea in infants and young animals worldwide. rvas have also been detected in several wild and zoo animals, indicating wide susceptibility of wild animals. however, the role of wild animals in the infection cycle of rvas is unclear. wild boars are indigenous in many countries in the world. japanese wild boars (sus scrofa leucomystax) have been migrating close to human habitats in japan, indicating the possibility of natural tra ... | 2013 | 23958658 |
| detection of bovine group a rotavirus using rapid antigen detection kits, rt-pcr and next-generation dna sequencing. | we investigated the sensitivity of human rotavirus rapid antigen detection (rad) kits, rt-pcr and next-generation dna sequencing (ngs) for detection of bovine group a rotavirus (rva). the dipstick 'eiken' rota (dipstick) showed the highest sensitivity out of the seven rad kits against all selected strains in limited dilution analyses, which was consistent with the results of equine rotavirus previously reported. rt-pcr had 10(0)-10(3)-fold higher sensitivity than the dipstick. the ngs using rt-p ... | 2013 | 23912876 |
| group a rotavirus and norovirus display sharply distinct seasonal profiles in belém, northern brazil. | several viruses have been associated with acute gastroenteritis (age), and group a rotavirus (rva) and norovirus (nov) are the most prevalent. this study aimed to assess their prevalence among children hospitalised for diarrhoea during a three-year surveillance study. from may 2008-april 2011, overall positivity rates of 21.6% (628/2904) and 35.4% (171/483) were observed for rva and nov, respectively. the seasonality observed indicated distinct patterns when both viruses were compared. this find ... | 2013 | 23903985 |
| case-control study of microbiological etiology associated with calf diarrhea. | calf diarrhea is a major economic burden for the us cattle industry. a variety of infectious agents are implicated in calf diarrhea and co-infection of multiple pathogens is not uncommon in diarrheic calves. a case-control study was conducted to assess infectious etiologies associated with calf diarrhea in midwest cattle farms. a total of 199 and 245 fecal samples were obtained from diarrheic and healthy calves, respectively, from 165 cattle farms. samples were tested by a panel of multiplex pcr ... | 2013 | 23886509 |
| homologous 2',5'-phosphodiesterases from disparate rna viruses antagonize antiviral innate immunity. | efficient and productive virus infection often requires viral countermeasures that block innate immunity. the ifn-inducible 2',5'-oligoadenylate (2-5a) synthetases (oass) and ribonuclease (rnase) l are components of a potent host antiviral pathway. we previously showed that murine coronavirus (mhv) accessory protein ns2, a 2h phosphoesterase superfamily member, is a phosphodiesterase (pde) that cleaves 2-5a, thereby preventing activation of rnase l. the pde activity of ns2 is required for mhv re ... | 2013 | 23878220 |
| novel g10p[14] rotavirus strain, northern territory, australia. | we identified a genotype g10p[14] rotavirus strain in 5 children and 1 adult with acute gastroenteritis from the northern territory, australia. full genome sequence analysis identified an artiodactyl-like (bovine, ovine, and camelid) g10-p[14]-i2-r2-c2-m2-a11-n2-t6-e2-h3 genome constellation. this finding suggests artiodactyl-to-human transmission and strengthens the need to continue rotavirus strain surveillance. | 2013 | 23876354 |
| detection of unusual g6 rotavirus strains in italian children with diarrhoea during the 2011 surveillance season. | two rare g6 rotavirus a (rva) strains, designated as rva/human-wt/ita/cec06/2011/g6p[6] and rva/human-wt/ita/pg05/2011/g6p[9], were identified in stool specimens from children hospitalized in central italy. after pcr genotyping, the samples cec06 and pg05 gave g-ud-p[6] and g-ud-p[9] genotypes, respectively. to determine the g-type and to characterize further the two strains, sequencing of 8 of the 11 genomic segments was performed. cec06 and pg05 strains were found to possess unusual genotype c ... | 2013 | 23868788 |
| sensitive and specific quantitative detection of rotavirus a by one-step real-time reverse transcription-pcr assay without antecedent double-stranded-rna denaturation. | a real-time quantitative reverse transcription-pcr (qrt-pcr) assay using the recombinant thermostable thermus thermophilus (rtth) enzyme was developed to detect and quantify rotavirus a (rva). by using rtth polymerase, significant improvement was achieved over the existing real-time rt-pcr assays, which require denaturation of the rva double-stranded rna (dsrna) prior to assay setup. using a dsrna transcript for segment 7, which encodes the assay target nsp3 gene, the limit of detection for the ... | 2013 | 23850952 |
| evaluation of luminex xtag gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. | acute diarrheal disease (add) can be caused by a range of pathogens, including bacteria, viruses, and parasites. conventional diagnostic methods, such as culture, microscopy, biochemical assays, and enzyme-linked immunosorbent assays (elisa), are laborious and time-consuming and lack sensitivity. combined, the array of tests performed on a single specimen can increase the turnaround time (tat) significantly. we validated a 19plex laboratory-developed gastrointestinal pathogen panel (gpp) using l ... | 2013 | 23850948 |
| rabbit colony infected with a bovine-like g6p[11] rotavirus strain. | group a rotaviruses (rvas) are the main etiological agent of infantile diarrhea in both humans and animals worldwide. a limited number of studies have investigated the molecular characteristics of rva strains in stool specimens of rabbits, with only a few lapine rva strains isolated and (partially) characterized to date. the most common g/p-genotype combinations found in rabbits are g3p[14] and g3p[22]. in this study a rva strain was isolated from the small intestine of a 9-week-old rabbit from ... | 2013 | 23830050 |
| zoonotic transmission of reassortant porcine g4p[6] rotaviruses in hungarian pediatric patients identified sporadically over a 15 year period. | genotype g4p[6] rotavirus a (rva) strains collected from children admitted to hospital with gastroenteritis over a 15 year period in the pre rotavirus vaccine era in hungary were characterized in this study. whole genome sequencing and phylogenetic analysis was performed on eight g4p[6] rva strains. all these rva strains shared a fairly conservative genomic configuration (g4-p[6]-i1/i5-r1-c1-m1-a1/a8-n1-t1/t7-e1-h1) and showed striking similarities to porcine and porcine-derived human rva strain ... | 2013 | 23792183 |
| [genetic diversity of rotavirus group a: correlation between g3 type and severity of the infection. valencia, venezuela]. | genetic and antigenic rotavirus (rv) variabilities may have implications in the severity of the infection caused by these agents; however the studies are not conclusive. for that purpose, the mean severity scores of diarrhea episodes caused by rv types g1 and g3 were compared, at ciudad hospitalaria "dr. enrique tejera" in valencia, venezuela, between 2001-2005. rv were identified by elisa, g and p types by rt-pcr. the severity of infection was determined using the ruuska-vesikari system and the ... | 2013 | 23781711 |
| rotavirus shedding in symptomatic and asymptomatic children using reverse transcription-quantitative pcr. | reverse transcription-real-time polymerase chain reaction (rt-qpcr) for the vp6 gene was used to study group a rotavirus shedding in children with symptomatic and asymptomatic rotavirus infection. sequential stool samples (n = 345) from 10 children with rotavirus associated diarrhea and from five children (n = 161) with asymptomatic rotavirus infection were collected over a period of 2 months. a rt-qpcr assay on the samples using a rotavirus vp6 plasmid standard demonstrated high reproducibility ... | 2013 | 23775335 |
| continuous genotyping for human rotavirus group a. | no abstract available. | 2012 | 23770884 |
| human g9p[8] rotavirus strains circulating in cameroon, 1999-2000: genetic relationships with other g9 strains and detection of a new g9 subtype. | group a rotaviruses (rv-a) are the leading cause of viral gastroenteritis in children worldwide and genotype g9p[8] is one of the five most common genotypes detected in humans. in order to gain insight into the degree of genetic variability of g9p[8] strains circulating in cameroon, stool samples were collected during the 1999-2000 rotavirus season in two different geographic regions in cameroon (southwest and western regions). by rt-pcr, 15 g9p[8] strains (15/89=16.8%) were identified whose gen ... | 2013 | 23770141 |
| simultaneous detection of group a rotavirus in swine and rat on a pig farm in brazil. | this study investigated the occurrence of rotavirus in porcine and rattus norvegicus, at the same time, on a pig farm in the city of jaguariúna, são paulo, brazil. swine (n = 21) and rat (n = 6) fecal samples were analyzed by nested rt-pcr assay. rotavirus occurred in seven porcine and two rat samples. a total of three pig and one rat samples were further submitted to genetic sequencing. the partial nsp5 gene phylogeny showed that all strains were segregated in the genotype h1. these results poi ... | 2013 | 23766702 |
| one year survey of human rotavirus strains suggests the emergence of genotype g12 in cameroon. | in this study the emergence of rotavirus a genotype g12 in children <5 years of age is reported from cameroon during 2010/2011. a total of 135 human stool samples were p and g genotyped by reverse transcriptase pcr. six different rotavirus vp7 genotypes were detected, including g1, g2, g3, g8, g9, and g12 in combinations with p[4], p[6] and p[8] vp4 genotypes. genotype g12 predominated in combination with p[8] (54.1%) and p[6] (10.4%) genotypes followed by g1p[6] (8.2%), g3p[6] (6.7%), g2p[4] (5 ... | 2013 | 23765785 |
| phylogenetic analyses of the vp4 and vp7 genes of porcine group a rotaviruses in sao paulo state, brazil: first identification of g5p[23] in piglets. | this study determined the group a rotavirus occurrence in pig farms from 7 different cities in são paulo state, brazil. out of 143 samples, 70 tested positive. sequence analyses of 37 strains indicated that the strains had the g3, g5, g9, and p[6], p[13]/p[22]-like, and p[23] genotypes. | 2013 | 23761160 |
| prevalence and molecular genotyping of group a rotaviruses in iranian children. | rotavirus is the leading cause of acute gastroenteritis in worldwide young children. effective vaccines to prevent rotavirus infection are currently available, although their clinical use is still limited, and rotavirus still causes many episodes of infantile gastroenteritis, mainly during the winter season. the aim of this study was to evaluate the prevalence of rotavirus infection in children aged <5-years-old who were hospitalised for gastroenteritis. one hundred and sixty-three stool samples ... | 2012 | 23729998 |
| molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients. | to describe the genotypic variability of group a rotavirus (rva) found in immunosuppressed and non-immunosuppressed pediatric patients treated at the hospital de clínicas da universidade federal do paraná (hc-ufpr), curitiba, paraná. | 2013 | 23684457 |
| whole-genomic analysis of g3p[23], g9p[23] and g3p[13] rotavirus strains isolated from piglets with diarrhea in thailand, 2006-2008. | group a rotavirus (rva) is the most common cause of severe acute viral gastroenteritis in humans and animals worldwide. this study characterized the whole genome sequences of porcine rvas, 2 g3p[23] strains (cmp40/08 and cmp48/08), 1 g9p[23] strain (cmp45/08), and 1 g3p[13] strain (cmp29/08). these strains were collected from diarrheic piglets less than 7weeks of age in 4 pig farms in chiang mai, thailand, in 2008. the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5 genes of cmp40/08 and cmp48/ ... | 2013 | 23681022 |
| recombinant monovalent llama-derived antibody fragments (vhh) to rotavirus vp6 protect neonatal gnotobiotic piglets against human rotavirus-induced diarrhea. | group a rotavirus (rva) is the leading cause of severe diarrhea in children. the aims of the present study were to determine the neutralizing activity of vp6-specific llama-derived single domain nanoantibodies (vhh nanoabs) against different rva strains in vitro and to evaluate the ability of g6p[1] vp6-specific llama-derived single domain nanoantibodies (vhh) to protect against human rotavirus in gnotobiotic (gn) piglets experimentally inoculated with virulent wa g1p[8] rotavirus. supplementati ... | 2013 | 23658521 |
| review of group a rotavirus strains reported in swine and cattle. | group a rotavirus (rva) infections cause severe economic losses in intensively reared livestock animals, particularly in herds of swine and cattle. rva strains are antigenically heterogeneous, and are classified in multiple g and p types defined by the two outer capsid proteins, vp7 and vp4, respectively. this study summarizes published literature on the genetic and antigenic diversity of porcine and bovine rva strains published over the last 3 decades. the single most prevalent genotype combina ... | 2013 | 23642647 |
| detection of rotavirus infection in bovine calves by rna-page and rt-pcr. | a total of 128 diarrhoeic faecal samples were collected from cattle and buffalo calves from pantnagar and dehradun during winter months. of the 110 cattle calves screened by rna-page, rotavirus was detected in 13 samples (11.81%) while no sample from buffalo calves was found positive. all samples were found to have long electropherotype and two distinct electropherotypes having segment variation were observed. the overall prevalence of rotavirus was 10.15% (13/128). rt-pcr targeting group specif ... | 2011 | 23637494 |
| feline origin of rotavirus strain, tunisia, 2008. | in tunisia in 2008, an unusual g6p[9] rotavirus, rva/human-wt/tun/17237/2008/g6p[9], rarely found in humans, was detected in a child. to determine the origin of this strain, we conducted phylogenetic analyses and found a unique genotype constellation resembling rotaviruses belonging to the feline ba222-like genotype constellation. the strain probably resulted from direct cat-to-human transmission. | 2013 | 23631866 |
| [the genetic stability of recombinant adenovirus expressing human rotavirus vp6 gene which used ad41 as vector]. | to investigate the genetic stability of non-replicating recombinant adenovirus which used ad41 as vector and could express vp6 gene of group a rotavirus during continous passage, in order to develop the vaccine of rotavirus. | 2012 | 23627019 |
| phylogenetic analyses of typical bovine rotavirus genotypes g6, g10, p[5] and p[11] circulating in argentinean beef and dairy herds. | group a rotavirus (rva) is one of the main causes of neonatal calf diarrhea worldwide. rva strains affecting argentinean cattle mainly possess combinations of the g6, g10, p[5] and p[11] genotypes. to determine rva diversity among argentinean cattle, representative bovine rva strains detected in diarrheic calves were selected from a survey conducted during 1997-2009. the survey covered the main livestock regions of the country from dairy and beef herds. different phylogenetic approaches were use ... | 2013 | 23624201 |
| mean protein evolutionary distance: a method for comparative protein evolution and its application. | proteins are under tight evolutionary constraints, so if a protein changes it can only do so in ways that do not compromise its function. in addition, the proteins in an organism evolve at different rates. leveraging the history of patristic distance methods, a new method for analysing comparative protein evolution, called mean protein evolutionary distance (meaped), measures differential resistance to evolutionary pressure across viral proteomes and is thereby able to point to the proteins' rol ... | 2013 | 23613826 |
| impact of rotavirus vaccine on rotavirus genotypes and caliciviruses circulating in french cattle. | group a rotaviruses are a leading cause of neonatal calf diarrhoea worldwide and prevention of this disease includes vaccination against these viruses. in order to highlight the potential selection of rotavirus genotypes due to immune pressure driven by vaccination, the aim of this study was to compare group a rotavirus genotypes circulating in french diarrhoeic calves in rotavirus vaccinated herds (g6p[5] vaccine) with those in non-vaccinated herds during one calving season in 2010. this study ... | 2013 | 23579256 |
| phylogenetic analysis of g1p[6] group a rotavirus strains detected in northeast brazilian children fully vaccinated with rotarix™. | in 2009 the world health organization recommended the use of group a rotavirus (rva) vaccines in all national immunization programs (nips) in order to control severe rva gastroenteritis disease. in brazil, rotarix™ was introduced in the nip in march 2006, and a significant reduction in mortality rates among children ≤ 5 years old was observed, especially in the northern and northeastern brazil. in the current study the 11 gene segments of six brazilian g1p[6] rva strains, isolated in 2009 and 20 ... | 2013 | 23538335 |
| continued circulation of g12p[6] rotaviruses over 28 months in nepal: successive replacement of predominant strains. | rotavirus a causes severe diarrhoea in infants and young children worldwide. the migration pattern (electropherotype) of the double-stranded rna genome upon polyacrylamide gel electrophoresis has been used to define "strains" in molecular epidemiology. in temperate countries, distinct electropherotypes (strains) appear after the annual, off-seasonal interruption of rotavirus circulation. in nepal, rotavirus circulated year-round and an uncommon genotype g12p[6] predominated and persisted, provid ... | 2013 | 23533063 |
| evidence for genetic reassortment between human rotaviruses by full genome sequencing of g3p[4] and g2p[4] strains co-circulating in india. | rotavirus a causes severe diarrhoea in infants and young children worldwide. many unusual combinations of g and p genotypes have been observed in rotaviruses circulating in developing countries. mixed infection of a single individual with more than one strain is a mechanism by which genetic reassortants are formed with unusual g and p combinations. however, few studies have provided direct evidence for the formation of such unusual strains as a result of co-infection of co-circulating strains. h ... | 2013 | 23532829 |
| histophilus somni-induced infections in cattle from southern brazil. | the sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern brazil was investigated. two histophilus somni-associated syndromes were identified: systemic histophilosis (n = 4) and abortion (n = 1). the principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. pcr assays were used to amplify specific amplicons of the ovine herpesvirus 2, ... | 2013 | 23526124 |
| detection and characterisation of rotaviruses from children less than 5 years hospitalised with acute gastroenteritis in nagercoil. | group a rotavirus continues to be the major cause of severe gastroenteritis in young children in developing countries. in this study, we report the prevalence and genotype of rotaviruses identified from children <5 years of age hospitalised with acute gastroenteritis from nagercoil, tamil nadu from 2007-2010. from the 139 children included in the study, 71 samples (51%) were positive by elisa and 65 samples were positive by pcr-based methods. g1p[8] (44.6%) was the most commonly identified genot ... | 2016 | 23508433 |
| occurrence and investigation of enteric viral infections in pigs with diarrhea in china. | between february 2011 and february 2012, 985 and 324 samples were collected from diarrheal and healthy pigs, respectively, to detect porcine epidemic diarrhea virus (pedv), porcine kobuvirus (pkov), porcine bocavirus (pbov), porcine group a rotavirus (garv), and transmissible gastroenteritis virus (tgev). pedv and pkov clearly predominated in diarrheal pigs. compared to healthy pigs, a substantial prevalence of mixed infections was observed in diarrheal pigs (72.3 %) (p < 0.001). all of the coin ... | 2013 | 23494226 |
| identification of cellular calcium binding protein calmodulin as a regulator of rotavirus a infection during comparative proteomic study. | rotavirus (rv) being the major diarrhoegenic virus causes around 527000 children death (<5 years age) worldwide. in cellular environment, viruses constantly adapt and modulate to survive and replicate while the host cell also responds to combat the situation and this results in the differential regulation of cellular proteins. to identify the virus induced differential expression of proteins, 2d-dige (two-dimensional difference gel electrophoresis) based proteomics was used. for this, ht-29 cell ... | 2013 | 23437200 |