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hyphomonas atlanticus sp. nov., isolated from the atlantic ocean and emended description of the genus hyphomonas.a taxonomic study was carried out on strains 22ii1-22f38(t) and 22ii-s13e, which were isolated from sea water and sediment from the atlantic ocean, respectively. the two strains were gram-negative, oxidase and catalase positive, oval to pear shaped, and motile by a single polar flagellum. phylogenetic analysis based on 16s rrna gene sequences indicated that both strains belonged to the genus hyphomonas, with highest sequence similarity (98.2%) to the type strains h. jannaschiana dsm 5153(t) and ...201425066824
an unusual cause of recurrent chylothorax: gorham syndrome. 201425057476
redescription of platynaspisflavoguttata (gorham) (coleoptera, coccinellidae) and notes on nomenclature of platynaspiskapuri chakraborty & biswas.platynaspisflavoguttata (gorham) (coleoptera: coccinellidae) is redescribed and the male genitalia are illustrated for the first time. it is also recorded from sri lanka for the first time. platynaspisbimaculata (hoang, 1983) is a new junior synonym of platynaspisbimaculata pang & mao, 1979 (new synonym). platynaspiskapuri chakraborty & biswas, 2000, the replacement name for platynaspisbimaculata pang & mao, 1979 established by ukrainsky (2007), is also the new replacement name for platynaspisbi ...201425057247
comparison of the effects of blending and juicing on the phytochemicals contents and antioxidant capacity of typical korean kernel fruit juices.four korean kernel fruit (apple, pear, persimmon, and mandarin orange) juices were obtained by household processing techniques (i.e., blending, juicing). whole and flesh fractions of each fruit were extracted by a blender or a juicer and then examined for phytochemical content (i.e., organic acids, polyphenol compounds). the antioxidant capacity of each juice was determined by ferric reducing antioxidant power (frap) and 2,2-diphenyl-1-picrylhydrazyl (dpph) assays. results revealed that juices t ...201425054109
steam explosion treatment for ethanol production from branches pruned from pear trees by simultaneous saccharification and fermentation.this study investigated the production of ethanol from unutilized branches pruned from pear trees by steam explosion pretreatment. steam pressures of 25, 35, and 45 atm were applied for 5 min, followed by enzymatic saccharification of the extracted residues with cellulase (cellic ctec2). high glucose recoveries, of 93.3, 99.7, and 87.1%, of the total sugar derived from the cellulose were obtained from water- and methanol-extracted residues after steam explosion at 25, 35, and 45 tm, respectively ...201425036499
a proteomics sample preparation method for mature, recalcitrant leaves of perennial plants.sample preparation is key to the success of proteomics studies. in the present study, two sample preparation methods were tested for their suitability on the mature, recalcitrant leaves of six representative perennial plants (grape, plum, pear, peach, orange, and ramie). an improved sample preparation method was obtained: tris and triton x-100 were added together instead of chaps to the lysis buffer, and a 20% tca-water solution and 100% precooled acetone were added after the protein extraction ...201425028960
characterization and regulation of the resistance-nodulation-cell division-type multidrug efflux pumps mdtabc and mdtuvw from the fire blight pathogen erwinia amylovora.the gram-negative bacterium erwinia amylovora is the causal agent of the devastating disease fire blight in rosaceous plants such as apple, pear, quince, raspberry, and cotoneaster. in order to survive and multiply in a host, microbes must be able to circumvent the toxic effects of antimicrobial plant compounds, such as flavonoids and tannins. e. amylovora uses multidrug efflux transporters that recognize and actively export toxic compounds out of the cells. here, two heterotrimeric resistance-n ...201425012600
growth medium alterations improve in vitro cold storage of pear germplasm.development of new fruit cultivars is dependent on genetic resource collections such as those at the pomological garden of the institute of horticulture and viticulture near almaty, kazakhstan. the pear germplasm collection of the pomological garden contains 615 cultivars and three species. in vitro cold storage of the collection would provide additional security to the field collection.201424997837
gorham-stout disease mimicking spondyloarthritis. 201424986965
proteogenomic analysis of the venturia pirina (pear scab fungus) secretome reveals potential effectors.a proteogenomic analysis is presented for venturia pirina, a fungus that causes scab disease on european pear (pyrus communis). v. pirina is host-specific, and the infection is thought to be mediated by secreted effector proteins. currently, only 36 v. pirina proteins are catalogued in genbank, and the genome sequence is not publicly available. to identify putative effectors, v. pirina was grown in vitro on and in cellophane sheets mimicking its growth in infected leaves. secreted extracts were ...201424965097
improvement of a dry formulation of pseudomonas fluorescens eps62e for fire blight disease biocontrol by combination of culture osmoadaptation with a freeze-drying lyoprotectant.to study the effect of lyoprotectants and osmoadaptation on viability of pseudomonas fluorescens eps62e during freeze-drying and storage and to evaluate the formulation in terms of efficacy in biocontrol and fitness on pear flowers.201424947806
arac/xyls family stress response regulators rob, soxs, plia, and opia in the fire blight pathogen erwinia amylovora.transcriptional regulators of the arac/xyls family have been associated with multidrug resistance, organic solvent tolerance, oxidative stress, and virulence in clinically relevant enterobacteria. in the present study, we identified four homologous arac/xyls regulators, rob, soxs, plia, and opia, from the fire blight pathogen erwinia amylovora ea1189. previous studies have shown that the regulators mara, rob, and soxs from escherichia coli mediate multiple-antibiotic resistance, primarily by upr ...201424936054
tc1-like transposable elements in plant genomes.the tc1/mariner superfamily of transposable elements (tes) is widespread in animal genomes. mariner-like elements, which bear a ddd triad catalytic motif, have been identified in a wide range of flowering plant species. however, as the founding member of the superfamily, tc1-like elements that bear a dd34e triad catalytic motif are only known to unikonts (animals, fungi, and entamoeba).201424926322
ultrastructure of plant leaf cuticles in relation to sample preparation as observed by transmission electron microscopy.the leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (tem) in only few studies. attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. with the aim of critically examining the effect of cuticle isolation and preparation for tem analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and european pear were assessed, followin ...201424895682
[is the wait-and-see approach in gorham syndrome a therapeutic option?]. 201424890889
notes on lycocerus kiontochananus (pic, 1921) and description of two new species of lycocerus gorham from china (coleoptera, cantharidae).the lycocerus kiontochananus species complex is revised; l. perroudi (pic, 1937) stat. rev., originally in cantharis, is resurrected from synonymy with l. kiontochananus (pic, 1921); l. atropygidialis (pic, 1937) stat. nov., originally in cantharis, is upgraded from cantharis perroudi var. atropygidialis pic, 1937 and resurrected from synonymy with l. kiontochananus; l. kejvali švihla, 2004 syn. nov. is considered as a junior synonym with l. kiontochananus. the male of l. perroudi and the female ...201424871519
nomenclatural notes on some checkered beetle (coleoptera: cleridae) types of the natural history museum, london (bmnh).lectotypes were designated (and holotypes and paralectotypes recognized) for 44 species of hydnocerinae, including the type species for isolemidia, parmius, paupris, allelidea, blaesiopthalmus and lemidia, four species of enoclerus (clerinae), and 14 species of cymatodera (tillinae). annotations include comments on additional type material, new type locality, previous (type series) locality, and questionable or mysterious types. phyllobaenus pallipes (gorham) and p. rufithorax (gorham) are synon ...201424870084
revision of the checkered beetle genus erymanthus spinola, 1841 (coleoptera, cleridae, clerinae).the  genus  erymanthus  spinola,  1841  is  taxonomically  revised  to  include  sixteen  species.  the species e. transversopustulatus n. sp. is described as new. erymanthus concoloripennis pic, 1932, n. syn. is placed in synonymy with e. bicolor chapin, 1924, e. horridus var. semirufus kuwert, 1893 is recognized as a distinct and valid species e. semirufus; e. variegatus pic, 1950 n. syn. is synonymized with e. pustulosus quedenfeldt, 1888. a key to the species, color photographs, genital figu ...201424869839
a new genus with six new species of typhlopolycystidinae evdonin, 1977 (platyhelminthes, kalyptorhynchia, polycystididae).five new species of the new taxon brunetorhynchus n. gen. are described: b. deconincki n. sp., b. microstylis n. sp. and b. complicatus n. sp. are from the mediterranean, b. canariensis n. sp. is from the canary island lanzarote, b. cannoni n. sp. is from the australian east coast and one species from the galapagos, formerly described as limipolycystis spec., is transferred to the new genus as b. dubius n. sp.. as in limipolycystis, these species have a single stylet, (accessory stylet type ii), ...201424869820
anaerobic co-digestion of dairy cattle manure and pear waste.anaerobic co-digestion of pre-treated dairy cattle manure (lcm) with pear waste after a storage period (plf) was tested at four inclusion levels: 0%, 25%, 75% and 100%. inclusion levels consisted in the replacement of the volatile solids (vs) from the lcm with the vs from plf keeping the organic loading rate around 1.1 ± 0.4 g svl(-1)d(-1). the introduction of the co-substrate clearly enhanced methane production rate (mpr) in comparison to single substrate (phase i) as phases ii and iii, respect ...201424865319
gorham-stout syndrome: a case report.gorham-stout syndrome, is an extremely rare disease of the bone, characterized for vascular and lymphatic channels proliferation in bony segments and consequent osseous resorption. there are around 200 cases reported around the world. although bisphosphonates are used for symptoms relief, there is no standardized treatment established. we present a case that was diagnosed in our centre secondary to a resistant epistaxis and a literature review of this condition.201424825048
phylogenetic analyses provide the first insights into the evolution of ovate family proteins in land plants.the ovate gene encodes a nuclear-localized regulatory protein belonging to a distinct family of plant-specific proteins known as the ovate family proteins (ofps). ovate was first identified as a key regulator of fruit shape in tomato, with nonsense mutants displaying pear-shaped fruits. however, the role of ofps in plant development has been poorly characterized.201424812252
identification of a pantoea biosynthetic cluster that directs the synthesis of an antimicrobial natural product.fire blight is a destructive disease of apple and pear caused by the enteric bacterial pathogen, erwinia amylovora. e. amylovora initiates infection by colonizing the stigmata of apple and pear trees, and entering the plants through natural openings. epiphytic populations of the related enteric bacterium, pantoea, reduce the incidence of disease through competition and antibiotic production. in this study, we identify an antibiotic from pantoea ananatis brt175, which is effective against e. amyl ...201424796857
tritrichomonas foetus: characterisation of ecto-phosphatase activities in the endoflagelar form and their possible participation on the parasite's transformation and cytotoxicity.the protist parasite tritrichomonas foetus displays a pear-shaped (ps) and a pseudocystic or endoflagellar form (eff). here, we characterised the ecto-phosphatase activity on the surface of eff and compare its biochemical properties to that of the ps regarding rate of substrate hydrolysis, ph activation profile and sensitivity to well-known phosphatases inhibitors. two strains exhibiting low- and high-cytotoxicity were used. the enzyme activities of ps and eff exhibited similar characteristics o ...201424793018
occurrence of patulin and its dietary intake through pear, peach, and apricot juices in italy.pear, peach, apricot, and mixed juices (125 samples) were purchased in italian supermarkets and organic food shops and analysed for patulin content. an overall incidence of 34.4% was observed in the fruit juices, with a mean contamination level of 3.6 µg kg(-1). no one sample exceeded 50 µg kg(-1), the maximum permitted limit according to european legislation. however, 19 positive samples contained more than 10 µg kg(-1) of patulin, which is the maximum level permitted for baby food. the inciden ...200824784810
antimicrobial potential of sicilian honeys against commensal escherichia coli and pathogenic salmonella serovar infantis.the purpose of this study was to investigate the antibacterial effect of 71 locally produced honeys from different botanical sources collected from apiarist's open markets in sicily.201324779285
γ-aminobutyric acid induces resistance against penicillium expansum by priming of defence responses in pear fruit.the results from this study showed that treatment with γ-aminobutyric acid (gaba), at 100-1000 μg/ml, induced strong resistance against blue mould rot caused by penicillium expansum in pear fruit. moreover, the activities of five defence-related enzymes (including chitinase, β-1,3-glucanase, phenylalnine ammonialyase, peroxidase and polyphenol oxidase) and the expression of these corresponding genes were markedly and/or promptly enhanced in the treatment with gaba and inoculation with p. expansu ...201424767023
hypovirulence of the phytopathogenic fungus botryosphaeria dothidea: association with a coinfecting chrysovirus and a partitivirus.botryosphaeria dothidea is an important pathogenic fungus causing fruit rot, leaf and stem ring spots and dieback, stem canker, stem death or stool mortality, and decline of pear trees. seven double-stranded rnas (dsrnas; dsrnas 1 to 7 with sizes of 3,654, 2,773, 2,597, 2,574, 1,823, 1,623, and 511 bp, respectively) were identified in an isolate of b. dothidea exhibiting attenuated growth and virulence and a sectoring phenotype. characterization of the dsrnas revealed that they belong to two dsr ...201424760881
gastrointestinal tract involvement of gorham's disease with expression of d2-40 in duodenum.we present a case of a 13-year-old boy with gorham's disease involving the thoracic and lumbar spine, femur, and gastrointestinal (gi) tract, which was complicated by recurrent chylothorax and gi bleeding. the presenting symptoms were intermittent abdominal pain, back pain, and melena. esophagogastroduodenoscopy and colonoscopy showed no abnormal lesions, but duodenal biopsy showed marked dilation of the lymphatics in the mucosa and submucosa, which revealed positive staining with a d2-40 immuno ...201424749089
glucitol dehydrogenase from peach (prunus persica) fruits is regulated by thioredoxin h.glucitol (gol) is a major photosynthetic product in plants from the rosaceae family. herein we report the molecular cloning, heterologous expression and characterization of gol dehydrogenase (goldhase, ec 1.1.1.14) from peach (prunus persica) fruits. the recombinant enzyme showed kinetic parameters similar to those reported for orthologous enzymes purified from apple and pear fruits. the activity of recombinant goldhase was strongly inhibited by cu(2+) and hg(2+), suggesting that it might have c ...201424747954
dissolved organic matter release and retention in ultisols in relation to land use patterns.the application of organic fertilizer to maintain soil fertility and crop yield has been practiced for thousands of years in china. this practice improves soil carbon sequestration, due to the high level of dissolved organic matter (dom) in organic manure. in this study, batch equilibrium studies were conducted to examine the capacity of three ultisols from areas under different land use patterns to retain dissolved organic carbon (doc) and dissolved organic nitrogen (don) extracted from rape ca ...201424704143
rare bone diseases and their dental, oral, and craniofacial manifestations.hereditary diseases affecting the skeleton are heterogeneous in etiology and severity. though many of these conditions are individually rare, the total number of people affected is great. these disorders often include dental-oral-craniofacial (doc) manifestations, but the combination of the rarity and lack of in-depth reporting often limit our understanding and ability to diagnose and treat affected individuals. in this review, we focus on dental, oral, and craniofacial manifestations of rare bo ...201424700690
the draft genome sequence of european pear (pyrus communis l. 'bartlett').we present a draft assembly of the genome of european pear (pyrus communis) 'bartlett'. our assembly was developed employing second generation sequencing technology (roche 454), from single-end, 2 kb, and 7 kb insert paired-end reads using newbler (version 2.7). it contains 142,083 scaffolds greater than 499 bases (maximum scaffold length of 1.2 mb) and covers a total of 577.3 mb, representing most of the expected 600 mb pyrus genome. a total of 829,823 putative single nucleotide polymorphisms ( ...201424699266
draft genome sequence of xylella fastidiosa pear leaf scorch strain in taiwan.the draft genome sequence of xylella fastidiosa pear leaf scorch strain pls229, isolated from the pear cultivar hengshan (pyrus pyrifolia) in taiwan, is reported here. the bacterium has a genome size of 2,733,013 bp, with a g+c content of 53.1%. the pls229 genome was annotated and has 3,259 open reading frames and 50 rna genes.201424652975
chemical composition and antibacterial activity of opuntia ficus-indica f. inermis (cactus pear) flowers.opuntia ficus-indica f. inermis (cactus pear) flowers have wide application in folk medicine. however, there are few reports focusing on their biological activity and were no reports on their chemical composition. the nutrient composition and hexane extracts of opuntia flowers at 4 flowering stages and their antibacterial and antifungal activities were investigated. the chemical composition showed considerable amounts of fiber, protein, and minerals. potassium (k) was the predominant mineral fol ...201424650181
antibacterial and antioxidant activities in extracts of fully grown cladodes of 8 cultivars of cactus pear.the antimicrobial and antioxidant activities of some cultivars of the nopal cactus have not been determined. in this study, 8 cultivars of nopal cacti from mexico were assayed for phenolic content, antioxidant activities, and antimicrobial activities against campylobacter jejuni, vibrio cholera, and clostridium perfringens. plant material was washed, dried, and macerated in methanol. minimum bactericidal concentrations (mbcs) were determined using the broth microdilution method. antioxidant acti ...201424621296
antimicrobial and antiradical activity of extracts obtained from leaves of three species of the genus pyrus.in this study, extracts were obtained from leaves of pyrus communis l., pyrus elaeagrifolia pall., and pyrus pyrifolia (bum.) nak. these extracts were tested for antiradical and antibacterial activity, as well as for the amount of total phenolic compounds, hydroquinone and arbutin. the antiradical activity was measured using 2,2-diphenyl-1-picrylhydrazyl radical and antibacterial activity with the disk diffusion method. the amount of phenolic compounds was determined using folin ciocalteu's phen ...201424621262
revision of the asian species of afidentula kapur, 1958 (coleoptera: coccinellidae: epilachnini).the asian species of afidentula kapur are revised, diagnosed and illustrated. the following new synonyms are proposed: afidentula stephensi (mulsant, 1850) (= afidentula erberi fürsch, 1984); afidentula thanhsonensis hoang, 1977 (=afidentula cucphuongensis hoang, 1977). the status of afidentula herbigrada (mulsant, 1850) as a synonym of afidentula bisquadripunctata (gyllenhal, 1808) is confirmed. one new species: afidentula semisqualens sp. nov. and three new subspecies: a. manderstjernae bielaw ...201324614446
complex regulation of secondary metabolism controlling pathogenicity in the phytopathogenic fungus alternaria alternata.the filamentous fungus alternaria alternata includes seven pathogenic variants (pathotypes), which produce different host-selective toxins and cause disease on different plants. the japanese pear, strawberry and tangerine pathotypes produce ak-toxin, af-toxin and act-toxin, respectively, which have a common structural moiety, 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid (eda). here, we identified a new gene, akt7 (ak-toxin biosynthetic gene 7), from the japanese pear pathotype, which encodes ...201424611558
viewpoints on vessels and vanishing bones in gorham-stout disease.gorham-stout disease (gsd) is a rare disorder characterized by the proliferation of endothelial-lined vessels in bone and the progressive destruction of bone. although jackson described the first case of gsd in 1838, the clinical and histological features of gsd were not defined until gorham and stout published their report on massive osteolysis in 1955. in the years since gorham and stout's groundbreaking publication, more than 300 cases of gsd have been described in the literature. these repor ...201424583233
case 203: gorham disease.a 77-year-old woman presented with pain in the right elbow of several years duration. there was no history of trauma, immunocompromise, or known malignancy. blood chemistry tests revealed an alkaline phosphatase level of 93 u/l (1.6 μkat/l) (30-120 u/l [0.5-2.0 μkat/l]), a sodium level of 139 mmol/l (135-147 mmol/l), a potassium level of 4.4 mmol/l (3.4-5.0 mmol/l), a calcium level of 2.38 mmol/l (2.2-2.55 mmol/l), a phosphate level of 1.81 mmol/l (0.85-1.5 mmol/l), a c-reactive protein level of ...201424568708
n-butyl sulfide as an attractant and coattractant for male and female codling moth (lepidoptera: tortricidae).research to discover and develop attractants for the codling moth, cydia pomonella l., has involved identification of the chemicals eliciting moth orientation to conspecific female moths, host fruits, fermented baits, and species of microbes. pear ester, acetic acid, and n-butyl sulfide are among those chemicals reported to attract or enhance attractiveness to codling moth. we evaluated the trapping of codling moth with n-butyl sulfide alone and in combination with acetic acid and pear ester in ...201424534117
conventional and real-time pcrs for detection of erwinia piriflorinigrans allow its distinction from the fire blight pathogen, erwinia amylovora.erwinia piriflorinigrans is a new pathogenic species of the bacterial genus erwinia that has been described recently in spain. accurate detection and identification of e. piriflorinigrans are challenging because its symptoms on pear blossoms are similar to those caused by erwinia amylovora, the causal agent of fire blight. moreover, these two species share phenotypic and molecular characteristics. two specific and sensitive conventional and real-time pcr protocols were developed to identify and ...201424509928
lectotype designations and nomenclatural changes in xylographus mellié (coleoptera, ciidae).we designate lectotypes and propose nomenclatural changes in xylographus mellié (coleoptera, ciidae) based on type specimens deposited in the museum of comparative zoology (usa), museum für naturkunde berlin (germany), the natural history museum (uk), muséum d'histoire naturelle de la ville de genève (switzerland), muséum national d'histoire naturelle (france), naturhistoriska riksmuseet (sweden) and naturhistorisches museum wien (austria). we designate lectotypes for the following species: cis ...201424493963
effects of pro-tex on zebrafish (danio rerio) larvae, adult common carp (cyprinus carpio) and adult yellowtail kingfish (seriola lalandi).aquaculture practices bring several stressful events to fish. stressors not only activate the hypothalamus-pituitary-interrenal-axis, but also evoke cellular stress responses. up-regulation of heat shock proteins (hsps) is among the best studied mechanisms of the cellular stress response. an extract of the prickly pear cactus (opuntia ficus indica), pro-tex, a soluble variant of tex-oe(®), may induce expression of hsps and reduce negative effects of cellular stress. pro-tex therefore is used to ...201424493298
the phenolic content and its involvement in the graft incompatibility process of various pear rootstocks (pyrus communis l.).this study investigates the influence of various rootstocks for pear on the phytochemical composition in the phloem above and below the graft union and the role of phenols in pear graft incompatibility. assays of phloem with cambium from 4-year-old 'conference', 'abate fetel' and 'williams' pear trees grafted on different rootstocks: quince ma, quince ba 29, fox 11, farold 40 (daygon), seedling pyrus communis l. and own rooted (p. communis l.) were analyzed with hplc-ms. the most abundant phenol ...201424484960
[electron microscopy and restriction analysis of bacteriophages isolated from quince and pear with symptoms of fire blight].phage populations of isolates from quince and pear affected with fire blight disease were studied using electron microscopy, restriction analysis and both agarose gel electrophoresis of particles and host range scoping method. the isolate from quince (pma1) comprises at least three phage populations and two phage variants that can be detected on different bacterial indicators. after titration of this isolate on erwinia amylovora the bacteriophage key of b1 morphotype with the genome size of 82.4 ...201424479316
gorham disease of mandible treated with post-operative radiotherapy. 201224455523
hydrogen sulfide prolongs postharvest storage of fresh-cut pears (pyrus pyrifolia) by alleviation of oxidative damage and inhibition of fungal growth.hydrogen sulfide (h2s) has proved to be a multifunctional signaling molecule in plants and animals. here, we investigated the role of h2s in the decay of fresh-cut pears (pyrus pyrifolia). h2s gas released by sodium hydrosulfide (nahs) prolonged the shelf life of fresh-cut pear slices in a dose-dependent manner. moreover, h2s maintained higher levels of reducing sugar and soluble protein in pear slices. h2s significantly reduced the accumulation of hydrogen peroxide (h2o2), superoxide radicals ( ...201424454881
analysis of mineral and heavy metal content of some commercial fruit juices by inductively coupled plasma mass spectrometry.the presence of potentially toxic elements and compounds in foodstuffs is of intense public interest and thus requires rapid and accurate methods to determine the levels of these contaminants. inductively coupled plasma mass spectrometry is a powerful tool for the determination of metals and nonmetals in fruit juices. in this study, 21 commercial fruit juices (apple, peach, apricot, orange, kiwi, pear, pineapple, and multifruit) present on romanian market were investigated from the heavy metals ...201324453811
control of plant defense mechanisms and fire blight pathogenesis through the regulation of 6-thioguanine biosynthesis in erwinia amylovora.fire blight is a devastating disease of rosaceae plants, such as apple and pear trees. it is characterized by necrosis of plant tissue, caused by the phytopathogenic bacterium erwinia amylovora. the plant pathogen produces the well-known antimetabolite 6-thioguanine (6tg), which plays a key role in fire blight pathogenesis. here we report that ycfr, a member of the lttr family, is a major regulator of 6tg biosynthesis in e. amylovora. inactivation of the regulator gene (ycfr) led to dramatically ...201424449489
localization of polysaccharides in isolated and intact cuticles of eucalypt, poplar and pear leaves by enzyme-gold labelling.the presence and characteristics of cuticle polysaccharides have been demonstrated by staining and spectroscopic methods, but their location in the cuticle remains unclear. furthermore, according to the prevailing model, polysaccharides are believed to be restricted to the cuticular layer and absent in the cuticle proper. with the aim of gaining insight into cuticular ultra-structure focussing on polysaccharides, cellulose and pectins have been identified and located in the transversal sections ...201424445334
characterization of acrd, a resistance-nodulation-cell division-type multidrug efflux pump from the fire blight pathogen erwinia amylovora.multidrug efflux pumps are membrane translocases that have the ability to extrude a variety of structurally unrelated compounds from the cell. acrd, a resistance-nodulation-cell division (rnd) transporter, was shown to be involved in efflux of highly hydrophilic aminoglycosides and a limited number of amphiphilic compounds in e. coli. here, a homologue of acrd in the plant pathogen and causal agent of fire blight disease erwinia amylovora was identified.201424443882
effect of different packaging materials and storage intervals on physical and biochemical characteristics of pear.the fruits of semi-soft pear (pyrus communis) cv. punjab beauty harvested at physiologically mature stage in 3rd week of july were packed in corrugated fiberboard boxes (cfb) with low density polyethylene (ldpe) liners, cfb with high density polyethylene (hdpe) liners, crates with ldpe liners, crates with hdpe liners, cfb, crates and wooden boxes and stored in walk-in-cool chamber at 0-1 °c and 90-95% rh. the fruits were analyzed after 30, 45, 60 and 75 days for physiological loss in weight (plw ...201324425900
water permeability of isolated cuticular membranes: the effect of cuticular waxes on diffusion of water.the water permeability of astomatous cuticular membranes isolated from citrus aurantium l. leaves, pear (pyrus communis l.) leaves and onion (allium cepa l.) bulb scales was determined before and after extraction of cuticular waxes with lipid solvents. in pear, the permeability coefficients for diffusion of tritiated water across cuticular membranes (cm) prior to extraction [p d(cm)] decreased by a factor of four during leaf expansion. in all three species investigated p d(cm) values of cuticula ...197624424766
production of single-cell oil from prickly-pear juice fermentation by cryptococcus curvatus grown in batch culture.the biomass of cryptococcus curvatus, an oleaginous yeast, reached 11 g/l and accumulated 46% (w/w) lipid when grown for 35 h in batch culture on diluted (25%) prickly-pear juice. the c:n ratio of the juice was about 50 g/g. the efficiency of substrate conversion was 0.48 g/g for biomass and 0.22 g/g for lipids. the extracted lipids were mainly oleic (18:1) and palmitic (16:0) acids and the quality of pipid approached that of palm oil.199424421128
asymmetrical jelly secretion of zygotes of pelvetia and fucus: an early polarization event.five hours before germination the zygotes of pelvetia fastigiata adhere to their substrate. a jelly layer covers the entire cell but most of the transparent jelly, artificially outlined by a layer of resin beads, is secreted at the prospective rhizoid pole. if the direction of the growth-orienting light is shifted after the asymmetrical secretion has already started, the direction of the secretion is also shifted. the polarization axis can be predicted by the site of the intensive jelly secretio ...197824414363
concanavalin a-mediated agglutination of plant plastids.cucumber (cucumis sativus l.) and pear (pyrus domestica medik.) fruit proplastids, and pea (pisum sativum l., cv. meteor) leaf chloroplasts, extracted by osmotic rupture of protoplasts isolated after degradation of the cell walls by cellulase and pectinase, agglutinated in the presence of con a. agglutination of cucumber proplastids was inhibited by anti-con a and by methyl α d-gluco/manno pyranosides but not by methyl α d-galactopyranoside. fluorescein isothiocyanate-conjugated con a (fitc-con ...197824413938
taxonomy and phylogenetic position of fomitiporia torreyae, a causal agent of trunk rot on sanbu-sugi, a cultivar of japanese cedar (cryptomeria japonica) in japan.trunk rot poses a substantial threat to sanbu-sugi, one of the most economically important cultivars of japanese cedar (cryptomeria japonica). the etiology of this disease, including its main agents, is incompletely known. this trunk rot was attributed to fomitiporia (phellinus) hartigii or f. (phellinus) punctata. here we phylogenetically analyzed dna sequences of four markers from a set of strains isolated from trunk-rot symptoms and recovered a single, monophyletic clade, indicating that a si ...201424396106
hypolipidaemic foods in china.with the changes in dietary pattern in china, in recent years, hyperlipidaemia has become an important problem in the pathogenesis of chronic degenerative diseases, especially the cardio-cerebro-vascular diseases. from studies on laboratory animals and people with hyperlipidaemia, a number of hypolipidaemic foods and beverages have been identified, of relevance to traditional chinese food culture. their absence from the diet may, in part account for the increasing prevalence of hyperlipidaemia i ...199624394619
diffuse lymphangiomatosis: gorham-stout syndrome. 201424390534
agrobacterium tumefaciens-mediated transformation of valsa mali: an efficient tool for random insertion mutagenesis.valsa mali is a causal agent of apple and pear trees canker disease, which is a destructive disease that causes serious economic losses in eastern asia, especially in china. the lack of an efficient transformation system for valsa mali retards its investigation, which poses difficulties to control the disease. in this research, a transformation system for this pathogen was established for the first time using a. tumefaciens-mediated transformation (atmt), with the optimal transformation conditio ...201324381526
flavorings as new sources of contamination by deteriogenic alicyclobacillus of fruit juices and beverages.this study aimed to report the incidence of alicyclobacillus and alicyclobacillus acidoterrestris in apple and pear flavorings (n=42) and to assess the effect of guaiacol-producing a. acidoterrestris strains on apple flavorings stored at 4, 20 and 45 °c. a real-time polymerase chain reaction (rt-pcr) method was used for simultaneous confirmation of alicyclobacilli. a total of six isolates were identified as a. acidoterrestris, and only one strain was not able to produce guaiacol. the storage of ...201424370970
the relationship between the morphology and structure and the quality of fruits of two pear cultivars (pyrus communis l.) during their development and maturation.the flavour and nutritional values of pears are appreciated by consumers worldwide, who, however, demand specific fruit quality, that is, attractive appearance, firmness and flavour, and health safety as well as long-term shelf life and storability. pear cultivars differ in terms of the above-mentioned traits; therefore, we undertook investigations to demonstrate the differences in structure of fruits of two pear cultivars that determine fruit quality in its broadest sense. the micromorphology, ...201324327806
comparison of tannin levels in developing fruit buds of two orchard pear varieties using two techniques, folin-denis and protein precipitation assays.as part of a study into dietary selection by avian pest species, the levels of phenolic material in pear buds have been determined using two approaches, the folin-denis assay and an assay based on the precipitation of bovine serum albumin by astringent phenols. the results of these two assays do not correlate over the sampling period and their overall significance to ecological studies is discussed.198424318554
characterization of anatolian traditional quince cultivars, based on microsatellite markers.we conducted simple sequence repeat (ssr) analyses of 15 traditional quince (cydonia oblonga) cultivars from anatolian gene sources for molecular characterization and investigation of genetic relationships. three pear and two apple cultivars were used as references for ssr locus data analysis and to determine allele profiles between species. eight ssr loci that were developed from apple and pear were used, and a total of 44 alleles were found among quince cultivars. the ch01f02 locus was found t ...201324301958
the genus aspidimerus mulsant, 1850 (coleoptera, coccinellidae) from china, with descriptions of two new species.chinese members of the genus aspidimerus mulsant, 1850 are reviewed. ten species are recognized, including two new species: a. zhenkangicus huo & ren, sp. n. and a. menglensis huo & ren, sp. n. a. kabakovi hoàng is recorded from china for the first time. a. blandus (mader, 1954) is recognized as synonymous with a. ruficrus gorham, 1895 (syn. n.). aspidimerus rectangulatus kuznetsov & pang, 1991 and a. serratus kuznetsov & pang, 1991 are transferred to the genus pseudaspidimerus kapur, 1948 (comb ...201324294073
effectiveness of a bacteriophage in reducing listeria monocytogenes on fresh-cut fruits and fruit juices.listeria monocytogenes is a serious foodborne pathogen and new strategies to control it in food are needed. among them, bacteriophages hold attributes that appear to be attractive. the objective of this study was to investigate the efficacy of the bacteriophage listex p100 to control l. monocytogenes growth on melon, pear and apple products (juices and slices) stored at 10 °c. l. monocytogenes grew well in untreated fruit slices. in juices, the pathogen grew in untreated melon, survived in untre ...201424290636
biomolecular characterization of the levansucrase of erwinia amylovora, a promising biocatalyst for the synthesis of fructooligosaccharides.erwinia amylovora is a plant pathogen that affects rosaceae, such as apple and pear. in e. amylovora the fructans, produced by the action of a levansucrase (ealsc), play a role in virulence and biofilm formation. fructans are bioactive compounds, displaying health-promoting properties in their own right. their use as food and feed supplements is increasing. in this study, we investigated the biomolecular properties of ealsc using hpaec-pad, maldi-tof ms, and spectrophotometric assays. the enzyme ...201324274651
agrobacterium-mediated genetic transformation using cotyledons in japanese pear (pyrus pyrifolia).genetic transformation was successfully established producing both transformed adventitious shoots and calli in japanese pear (pyrus pyrifolia nakai) by using cotyledons as explants. cotyledons of five cultivars were co-cultivated with agrobacterium tumefaciens strain lba4404 carrying the pbin19-sgfp, which contained a green fluorescent protein gene and the neomycin phosphotransferase gene. in order to increase transformation efficiency, sonication and ethylenedioxybis (ethylamine)-n,n,n',n'-tet ...201324273422
simultaneous detection and differentiation of three viruses in pear plants by a multiplex rt-pcr.a multiplex rt-pcr (mrt-pcr) assay was developed for detection and differentiation of the apple stem pitting virus (aspv), apple stem grooving virus (asgv) and apple chlorotic leaf spot virus (aclsv), which are viruses frequently occurring in pear trees. different combinations of mixed primer pairs were tested for their specificity and sensitivity for the simultaneous detection of the three viruses. three primer pairs were used to amplify their fragments of 247bp, 358bp and 500bp, respectively. ...201424269332
a case of an idiopathic massive osteolysis with skip lesions.a patient with a 2-year history of pain in the left arm, and decreased strengths unrelieved by non-steroidal anti-inflammatory therapy, was being referred for repeating radiography. the radiologic examinations have demonstrated a unique pattern of non-contiguous osteolysis in the left elbow, proximal and distal radius, ulna, wrist, carpal bones, proximal and distal metacarpals and phalanges. multi-site biopsies were being performed and confirmed the diagnosis of massive osteolysis. to our knowle ...201624265571
vacuolar yield and alteration with aging of suspension-cultured cells from pear fruit.vacuoles were isolated from suspension-cultured pear fruit (pyrus communis l. passe crassane) cells of various ages. yield of vacuoles averaged 17% of protoplasts throughout all the experimental stages. the isolated vacuoles were progressively larger and more likely to stain with neutral red as cells advanced from division to growth and incipient senescence. a relationship between aging and vacuolar or membrane function was implicit in the size and staining characteristics of isolated vacuoles.198724248664
adventitious shoot regeneration from leaf tissue of three pear (pyrus sp.) cultivars in vitro.to develop an adventitious regeneration system for pear cultivars, several experiments were conducted with 2 cultivars of pyrus communis l. ('seckel' and 'louise bonne') and one cultivar of p. bretschneideri rehd. ('crystal pear'). half-leaves, taken from shoots proliferating on lepoivre medium, were plated in petri-dishes on medium supplemented with various combinations of cytokinins and auxins. cultures of the above cultivars had been established from mature trees. among the growth regulators ...198924240463
plant regeneration from mesophyll protoplasts of williams' bon chretien (syn. bartlett) pear (pyrus communis l.).leaf protoplasts of axenic shoot cultures of pyrus communis l. cv. williams' bon chretien (syn. bartlett) underwent cell wall regeneration and division to give multicellular colonies in a modified murashige and skoog medium which lacked ammonium ions, but supplemented with 1-naphthaleneacetic acid (naa), 4-indole-3yl-acetic acid, 6-benzylaminopurine (bap) and casein hydrolysate. protoplast-derived colonies gave callus on murashige and skoog salts medium with naa and bap and exhibited shoot regen ...198824240424
efficacy of nonpheromone communication disruptants of codling moth (cydia pomonella): effect of pheromone isomers and of distance between calling females and dispensers.field tests comparing the ability of codlemone, (e,e)-8,10-dodecadien-1-ol, and isomers of codlemone to disrupt pheromonal communication of codling moth were carried out. in a pear orchard, four nonisomerizing, gray septa dispensers were placed in the upper canopy of each tree containing a trap baited with 10 virgin female codling moths. the dispensers were at trap height and 70 cm from the edge of each trap. trap catches of released male codling moths in three test areas were compared simultane ...199624227482
somatic hybridization of sexually incompatible top-fruit tree rootstocks, wild pear (pyrus communis var. pyraster l.) and colt cherry (prunus avium x pseudocerasus).mesophyll protoplasts of wild pear (pyrus communis var. pyraster l., pomoideae) were chemically fused with cell suspension protoplasts of cherry rootstock colt (prunus avium x pseudocerasus, prunoideae), following an electroporation treatment of the separate parental protoplast systems. fusion-treated protoplasts were cultured, on modified k8p medium, where it had been previously established that neither parental protoplasts were capable of division. somatic hybrid calli were recovered and, foll ...198924227027
evidence for quantitative responses during co-culture of pyrus communis protoplasts and erwinia amylovora.mesophyll protoplasts were isolated from axenic shoot cultures of pear cultivars, exhibiting different degrees of susceptibility to fire blight infection at the whole plant level and they were co-cultured with the wild-type strain cfbp 1430 of erwinia amylovora, and with an avirulent transposon mutant of the former (pmv 6046). results, as assessed in terms of the effects of bacteria on protoplast viability, the time to the onset of divisions, the percentage of the originally cultivated protoplas ...199024226825
analysis of the community structure of yeasts associated with the decaying stems of cactus. ii.opuntia species.a survey was made of yeast species associated with the decaying pads of 3 prickly pear cacti (opuntia phaeacantha, o. ficus-indica, ando. lindheimeri) in arizona and texas. yeast communities from 12 localities were compared among localities, amongopuntia species, and with previous data on yeast communities associated with columnar cacti. the results indicate thatopuntia necroses contain relatively more yeast species with broader physiological abilities in their communities than columnar necroses ...198324221704
analysis of the community structure of yeasts associated with the decaying stems of cactus. iii.stenocereus thurberi.yeast communities in necroses of organpipe cactus (stenocereus thurberi) were surveyed at 3 localities in arizona. quantitative analysis of random samples allows comparisons of the types and numbers of yeasts at 3 levels: within plants, between plants within a locality, and between localities. the analysis shows that the major source of variability is between plants. this pattern is identical with the pattern shown by agria cactus (stenocereus gummosus) and is thought to be due to sampling diffe ...198524221304
the ecology of the yeast flora in necroticopuntia cacti and of associateddrosophila in australia.a survey was made of the yeast communities isolated from necrotic tissue of 4 species of prickly-pear cacti (opuntia stricta, o. tomentosa, o. monacantha, ando. streptacantha) which have colonized in australia. yeast communities were sampled from a number of localities and at different times. cactus specific yeasts accounted for 80% of the total isolates, and the 3 most common species contributed 63% of the total. comparisons of the species compositions of the yeast communities indicated that th ...198424221180
combined effect of temperature and controlled atmosphere on storage and shelf-life of 'rocha' pear treated with 1-methylcyclopropene.the combination of temperature and atmosphere composition for storage of pyrus communis l. 'rocha' treated with 1-methylcyclopropene was investigated. fruits treated with 312 nl l(-1) 1-methylcyclopropene were stored at 0 ℃ and 2.5 ℃ in air and controlled atmosphere (ca) (3.04 kpa o2+ 0.91 kpa co2). fruits were removed from storage after 14, 26 and 35 weeks, transferred to shelf-life at approximately 22 ℃ and assessed for ripening and quality, symptoms of superficial scald and internal browning ...201524216324
novel molecular pathways in gorham disease: implications for treatment.rapid advances in evidence-based treatment schedules are a hallmark of modern oncology. in rare neoplastic diseases, however, clinical expertise is hard to build and evidence based on randomized trials almost impossible to collect. gorham disease is a rare form of lymphatic proliferation accompanied by osteolysis, which usually occurs in young adults. despite the fact that the clinical course of gorham disease is often devastating and occasionally fatal, insights into its biological background a ...201424214028
organogenesis from 'passe crassane' and 'old home' pear (pyrus communis l.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants.large numbers of highly viable mesophyll protoplasts were isolated from shoot cultures of the scion cv 'passe crassane' and the rootstock genotype 'old home' of common pear (pyrus communis l.). protoplasts were cultured for both genotypes either as liquid layers or as liquid-over-agar cultures, in ammonium-free ms medium with 0.5 m mannitol, 50 mg/l casein enzymatic hydrolysate (ceh), 2.0 mg/l naa and 1.0 mg/l bap, plus either 0.5 mg/l iaa (for 'old home') or 2.0 mg/l iaa (for 'passe crassane'). ...199224202928
a comparison of yeast communities found in necrotic tissue of cladodes and fruits ofopuntia stricta on islands in the caribbean sea and where introduced into australia.yeast communities growing in the decaying tissues (cladodes and fruits) ofopuntia stricta (prickly pear cactus) and associated yeast vectors (drosophila species) were compared in two geographic regions (caribbean and eastern australia). the australian yeast community provides an interesting comparison to the caribbean community, because the host planto. stricta was introduced to australia over 100 years ago. many of the yeasts found in the australian system also were introduced during a period o ...198724202645
two methods of haploidization in pear, pyrus communis l.: greenhouse seedling selection and in situ parthenogenesis induced by irradiated pollen.seedlings of 12 crosses involving pear varieties or hybrids were observed for the presence of haploid plants. on the basis of phenotypic characteristics, 17 plants corresponded to the haploid condition and, of these, 12 were determined by chromosome counting to be haploid (2n=x=17). in addition, and in order to induce in situ parthenogenesis, several pear varieties were pollinated with a selected clone carrying a homozygous dominant marker gene for the colour of red. this pollen had previously b ...199324190217
the use of luciferase as a reporter for response of plant cells to the fireblight pathogen erwinia amylovora.we have introduced the gene encoding luciferase from photinus pyralis into pear and tobacco cells in order to judge the reaction of plant tissue to damaging conditions such as incubation at high temperature or inoculation with a pathogen. the constitutive expression of the luciferase gene via a strong promoter slowly decreased during propagation of the transformed pear cell line. after various stress treatments the resulting luciferase activity and the atp content of the plant cells were determi ...199524185519
efficientagrobacterium-mediated transformation and recovery of transgenic plants from pear (pyrus communis l.).an efficient and reproducible method was established for genetic transformation of one pear variety (conferénce) usingagrobacterium tumefaciens-mediated gene transfer. wounded leaves of in vitro micropropagated plants were cocultivated with the disarmed strain eha101 harbouring the binary vector pfaj3000 carrying the chimaericnptii andgus genes. the protocol included a 3-6 month dark period on a regeneration medium solidified with gelrite, which contained 100 mg/l kanamycin. up to 42% of inocula ...199624177562
gorham disease of the lumbar spine with an abdominal aortic aneurysm: a case report.reports of gorham disease of the lumbar spine complicated by abdominal aortic aneurysms are rare.201424161363
identification of pyrus single nucleotide polymorphisms (snps) and evaluation for genetic mapping in european pear and interspecific pyrus hybrids.we have used new generation sequencing (ngs) technologies to identify single nucleotide polymorphism (snp) markers from three european pear (pyrus communis l.) cultivars and subsequently developed a subset of 1096 pear snps into high throughput markers by combining them with the set of 7692 apple snps on the irsc apple infinium® ii 8k array. we then evaluated this apple and pear infinium® ii 9k snp array for large-scale genotyping in pear across several species, using both pear and apple snps. t ...201324155917
taxonomic and nomenclatural notes on the genera themus motschulsky and lycocerus gorham (coleoptera, cantharidae).themus (s.str.) regalis (gorham, 1889), nom. rest.; themus (s.str.) scutulatus wittmer, 1983 = themus (s.str.) hmong kazantsev, 2007, syn. n.; themus (telephorops) coelestis (gorham, 1889) = themus violetipennis wang & yang, 1992, syn. n.; themus (telephorops) uniformis wittmer, 1983, stat. n. = themus (telephorops) cribripennis wittmer, 1983, syn. n.; themus (haplothemus) licenti pic, 1938, stat. rev., resurrected from synonymy with themus coriaceipennis (fairmaire, 1889); lycocerus aenescens ( ...201324146589
pear: a fast and accurate illumina paired-end read merger.the illumina paired-end sequencing technology can generate reads from both ends of target dna fragments, which can subsequently be merged to increase the overall read length. there already exist tools for merging these paired-end reads when the target fragments are equally long. however, when fragment lengths vary and, in particular, when either the fragment size is shorter than a single-end read, or longer than twice the size of a single-end read, most state-of-the-art mergers fail to generate ...201324142950
heat-induced oxidative injury contributes to inhibition of botrytis cinerea spore germination and growth.the inhibitory effect of heat treatment (ht) on botrytis cinerea, a major postharvest fungal pathogen, and the possible mode of action were investigated. spore germination and germ tube elongation of b. cinerea were both increasingly and significantly inhibited by ht (43 °c) for 10, 20 or 30 min. ht-induced gene expression of nadph oxidase a, resulted in the intracellular accumulation of reactive oxygen species. ht-treated b. cinerea spores exhibited higher levels of oxidative damage to proteins ...201424101365
syrian hamsters (mesocricetus auratus) with simultaneous intestinal giardia sp., spironucleus sp., and trichomonad infections.a commercial facility producing hamsters with a history of infection by dwarf tapeworm (hymenolepis nana) submitted 15 animals for necropsy and postmortem parasitological and microscopic examination. no tapeworms were detected grossly or microscopically. fecal examination including gastrointestinal mucosal smears demonstrated mixed intestinal bacteria and low numbers of giardia sp. histologic examination of small intestine demonstrated filling of the small intestinal crypts by large numbers of 7 ...201324081933
growth inhibition of erwinia amylovora and related erwinia species by neutralized short‑chain fatty acids.short-chain fatty acids (scfas) are used to preserve food and could be a tool for control of fire blight caused by erwinia amylovora on apple, pear and related rosaceous plants. neutralized acids were added to buffered growth media at 0.5–75 mm and tested at phs ranging from 6.8 to 5.5. particularly at low ph, scfas with a chain length exceeding that of acetic acid such as propionic acid were effective growth inhibitors of e. amylovora possibly due to uptake of free acid and its intracellular ac ...201324077735
gorham's disease of spine.gorham's disease is a rare disorder characterized by clinical and radiological disappearance of bone by proliferation of non-neoplastic vascular tissue. the disease was first reported by jackson in 1838 in a boneless arm. the disease was then described in detail in 1955 by gorham and stout. since then, about 200 cases have been reported in the literature, with only about 28 cases involving the spine. we report 2 cases of gorham's disease involving the spine and review related literature to gain ...201324066223
ultrasonography of the spleen, liver, gallbladder, caudal vena cava and portal vein in healthy calves from birth to 104 days of age.many of the ultrasonographic abdominal findings of adult cattle probably also apply to calves. however, significant changes associated with ruminal growth and transition from a milk to a roughage diet occur in young calves during the first few months, and it can be expected that ultrasonographic features of organs adjacent to the rumen such as spleen and liver also undergo significant changes. these have not been investigated to date and therefore the goal of this study was to describe ultrasono ...201324040969
rhizopus soft rot on pear (pyrus serotina) caused by rhizopus stolonifer in korea.rhizopus soft rot caused by rhizopus stolonifer occurred on pears (pyrus serotina) in the jinju city agricultural products wholesale market in korea from 2004 to 2005. the infection usually started from wounds due to cracking at harvest time. the lesions started as water-soaked, rapidly softened, then gradually expanded. the mycelia grew vigorously on the surface of the fruits and formed stolons. colonies on potato dextrose agar at 25℃ were white cottony to brownish black. sporangia were globose ...200624039490
evaluation of cinnamon essential oil microemulsion and its vapor phase for controlling postharvest gray mold of pears (pyrus pyrifolia).essential oil of cinnamon (cm) is a potential alternative to chemical fungicides. thus this work aimed to investigate the possible effects of cm microemulsions on decay developments and qualitative properties of pears.201424037944
a strategy to design efficient fermentation processes for traditional beverages production: prickly pear wine.this paper describes a methodology to establish an optimal process design for prickly pear wine production that preserves the peculiar and unique traits of traditional products, generating at the same time, technical information for appropriate design of both bioreactor and overall process. the strategy includes alcoholic fermentation optimization by the mixed native culture composed by pichia fermentans and saccharomyces cerevisiae, followed by malolactic fermentation optimization by oenococcus ...201324032574
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