Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| genetic characterization of circulating african swine fever viruses in nigeria (2007-2015). | sequencing and analysis of three discrete genome regions of african swine fever viruses (asfv) from archival samples collected in 2007-2011 and active and passive surveillance between 2012 and 2015 in nigeria were carried out. analysis was conducted by genotyping of three single-copy african swine fever (asf) genes. the e183l and b646l genes that encode structural proteins p54 and p72, respectively, were utilized to delineate genotypes before intragenotypic resolution by characterization of the ... | 2016 | 27480888 |
| the challenge of detecting classical swine fever virus circulation in wild boar (sus scrofa): simulation of sampling options. | classical swine fever (csf) is one of the most important viral diseases of domestic pigs ( sus scrofa domesticus) and wild boar ( sus scrofa ). for at least 4 decades, several european union member states were confronted with outbreaks among wild boar and, as it had been shown that infected wild boar populations can be a major cause of primary outbreaks in domestic pigs, strict control measures for both species were implemented. to guarantee early detection and to demonstrate freedom from diseas ... | 2016 | 27479901 |
| absence of autophagy promotes apoptosis by modulating the ros-dependent rlr signaling pathway in classical swine fever virus-infected cells. | a growing number of studies have demonstrated that both macroautophagy/autophagy and apoptosis are important inner mechanisms of cell to maintain homeostasis and participate in the host response to pathogens. we have previously reported that a functional autophagy pathway is trigged by infection of classical swine fever virus (csfv) and is required for viral replication and release in host cells. however, the interplay of autophagy and apoptosis in csfv-infected cells has not been clarified. in ... | 2016 | 27463126 |
| development of a duplex lateral flow assay for simultaneous detection of antibodies against african and classical swine fever viruses. | classical swine fever (csf) and african swine fever (asf) are both highly contagious diseases of domestic pigs and wild boar and are clinically indistinguishable. for both diseases, antibody detection is an integral and crucial part of prevention and control measures. the purpose of our study was to develop and initially validate a duplex pen-side test for simultaneous detection and differentiation of specific antibodies against csf virus (csfv) and asf virus (asfv). the test was based on the ma ... | 2016 | 27400954 |
| a mathematical model that simulates control options for african swine fever virus (asfv). | a stochastic model designed to simulate transmission dynamics of african swine fever virus (asfv) in a free-ranging pig population under various intervention scenarios is presented. the model was used to assess the relative impact of the timing of the implementation of different control strategies on disease-related mortality. the implementation of biosecurity measures was simulated through incorporation of a decay function on the transmission rate. the model predicts that biosecurity measures i ... | 2016 | 27391689 |
| genetic diversity of ruminant pestivirus strains collected in northern ireland between 1999 and 2011 and the role of live ruminant imports. | the genus pestivirus within the family flaviviridae includes bovine viral diarrhoea virus (bvdv) types 1 and 2, border disease virus (bdv) and classical swine fever virus. the two recognised genotypes of bvdv are divided into subtypes based on phylogenetic analysis, namely a-p for bvdv-1 and a-c for bvdv-2. | 2015 | 27354911 |
| a rapid strategy for the isolation of new faustoviruses from environmental samples using vermamoeba vermiformis. | the isolation of giant viruses is of great interest in this new era of virology, especially since these giant viruses are related to protists. giant viruses may be potentially pathogenic for many species of protists. they belong to the recently described order of megavirales. the new lineage faustovirus that has been isolated from sewage samples is distantly related to the mammalian pathogen african swine fever virus. this virus is also specific to its amoebal host, vermamoeba vermiformis, a pro ... | 2016 | 27341059 |
| pestivirus npro directly interacts with interferon regulatory factor 3 monomer and dimer. | interferon regulatory factor 3 (irf3) is a transcription factor involved in the activation of type i alpha/beta interferon (ifn-α/β) in response to viral infection. upon viral infection, the irf3 monomer is activated into a phosphorylated dimer, which induces the transcription of interferon genes in the nucleus. viruses have evolved several ways to target irf3 in order to subvert the innate immune response. pestiviruses, such as classical swine fever virus (csfv), target irf3 for ubiquitination ... | 2016 | 27334592 |
| different rna splicing mechanisms contribute to diverse infective outcome of classical swine fever viruses of differing virulence: insights from the deep sequencing data in swine umbilical vein endothelial cells. | molecular mechanisms underlying rna splicing regulation in response to viral infection are poorly understood. classical swine fever (csf), one of the most economically important and highly contagious swine diseases worldwide, is caused by classical swine fever virus (csfv). here, we used high-throughput sequencing to obtain the digital gene expression (dge) profile in swine umbilical vein endothelial cells (suvec) to identify different response genes for csfv by using both shimen and c strains. ... | 2016 | 27330868 |
| a synthetic molecular system capable of mirror-image genetic replication and transcription. | the overwhelmingly homochiral nature of life has left a puzzle as to whether mirror-image biological systems based on a chirally inverted version of molecular machinery could also have existed. here we report that two key steps in the central dogma of molecular biology, the template-directed polymerization of dna and transcription into rna, can be catalysed by a chemically synthesized d-amino acid polymerase on an l-dna template. we also show that two chirally mirrored versions of the 174-residu ... | 2016 | 27325097 |
| complete genome sequence of a novel sub-subgenotype 2.1g isolate of classical swine fever virus from china. | current subgenotype 2.1 isolates of classical swine fever virus (csfv) play a dominant role in csf outbreaks in china, and a novel sub-subgenotype 2.1g of csfv was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. in this study, complete genome of 2.1g isolate gd19/2011 collected from guangdong province of china in 2011 was sequenced. it was found to be 12,298 nucleotides (nt) in length, including a 375-nt 5'utr, a 11,697-nt opening reading ... | 2016 | 27318930 |
| hypervariable antigenic region 1 of classical swine fever virus e2 protein impacts antibody neutralization. | envelope glycoprotein e2 of classical swine fever virus (csfv) is the major antigen that induces neutralizing antibodies and confers protection against csfv infection. there are three hypervariable antigenic regions (har1, har2 and har3) of e2 that are different between the group 1 vaccine c-strain and group 2 clinical isolates. this study was aimed to characterize the antigenic epitope region recognized by monoclonal antibody 4f4 (mab-4f4) that is present in the group 2 field isolate hz1-08, bu ... | 2016 | 27317266 |
| pre-clinical evaluation of a real-time pcr assay on a portable instrument as a possible field diagnostic tool: experiences from the testing of clinical samples for african and classical swine fever viruses. | african swine fever (asf) and classical swine fever (csf) are two highly infectious transboundary animal diseases (tads) that are serious threats to the pig industry worldwide, including in china, the world's largest pork producer. in this study, a duplex real-time pcr assay was developed for the rapid detection and differentiation of african swine fever virus (asfv) and classical swine fever virus (csfv). the assay was performed on a portable, battery-powered pcr thermocycler with a low sample ... | 2016 | 27311689 |
| enhanced protective immunity of the chimeric vector-based vaccine radv-sfv-e2 against classical swine fever in pigs by a salmonella bacterial ghost adjuvant. | classical swine fever (csf) is a highly contagious swine disease caused by classical swine fever virus (csfv). previously, we demonstrated that radv-sfv-e2, an adenovirus-delivered, semliki forest virus replicon-vectored marker vaccine against csf, is able to protect pigs against lethal csfv challenge. from an economical point of view, it will be beneficial to reduce the minimum effective dose of the vaccine. this study was designed to test the adjuvant effects of salmonella enteritidis-derived ... | 2016 | 27301745 |
| classical swine fever virus ns5a protein changed inflammatory cytokine secretion in porcine alveolar macrophages by inhibiting the nf-κb signaling pathway. | classical swine fever (csf) caused by csf virus (csfv) is a highly contagious disease of the pigs. a number of studies have suggested that csfv non-structural (ns) 5a protein is involved in csfv-associated pathogenesis, but its mechanism is still uncertain. the aim of this study was to investigate the roles of ns5a protein in csfv-associated pathogenesis in cultured porcine alveolar macrophages (pams). | 2016 | 27296632 |
| establishment of a nanoparticle-assisted rt-pcr assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus. | porcine epidemic diarrhea virus (pedv) can cause serious disease and even death in neonatal piglets, resulting in serious damage to the swine industry worldwide. open reading frame 3 (orf3) is the only accessory gene in the pedv genome. previous studies have indicated that pedv vaccine strains have a partial deletion in orf3. in this study, a nanoparticle-assisted polymerase chain reaction (nanoparticle-assisted rt-pcr) assay targeting the orf3 of pedv was developed to distinguish pedv field str ... | 2016 | 27287433 |
| piglets with maternally derived antibodies from sows immunized with radv-sfv-e2 were completely protected against lethal csfv challenge. | classical swine fever (csf) is an economically important infectious disease of pigs caused by classical swine fever virus (csfv). to facilitate the eradication of csf in endemic areas, a marker vaccine enabling differentiation of infected from vaccinated animals (diva) is urgently needed. previously, we have demonstrated that the diva vaccine radv-sfv-e2, an adenovirus-vectored semliki forest virus replicon expressing the e2 glycoprotein of csfv, induces complete protection from lethal csfv chal ... | 2016 | 27283854 |
| establishment and characterization of a chimeric infectious cdna clone of classical swine fever virus. | classical swine fever virus (csfv) causes a highly contagious disease among swine that has an important economic impact worldwide. there are two important csfv strains in china, shimen and hog cholera lapinized virus (hclv). shimen strain is highly virulent while hclv, also referred to as c-strain, is a live attenuated vaccine strain considered to be one of the most effective and safest live vaccines. in this study, a chimeric infectious cdna clone of csfv named pt7sm-c was engineered by replaci ... | 2016 | 27265471 |
| diagnosis and genotyping of african swine fever viruses from 2015 outbreaks in zambia. | in early 2015, a highly fatal haemorrhagic disease of domestic pigs resembling african swine fever (asf) occurred in north western, copperbelt, and lusaka provinces of zambia. molecular diagnosis by polymerase chain reaction targeting specific amplification of p72 (b646l) gene of asf virus (asfv) was conducted. fourteen out of 16 domestic pigs from the affected provinces were found to be positive for asfv. phylogenetic analyses based on part of the p72 and the complete p54 (e183l) genes revealed ... | 2016 | 27247062 |
| identification of a new genotype of african swine fever virus in domestic pigs from ethiopia. | african swine fever (asf) is an important emerging transboundary animal disease (tad), which currently has an impact on many countries in africa, eastern europe, the caucasus and the russian federation. the current situation in europe shows the ability of the virus to rapidly spread, which stands to threaten the global swine industry. at present, there is no viable vaccine to minimize spread of the disease and stamping out is the main source of control. in february 2011, ethiopia had reported it ... | 2016 | 27211823 |
| african swine fever virus georgia isolate harboring deletions of 9gl and mgf360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge. | african swine fever virus (asfv) produces a contagious disease of domestic pigs that results in severe economic consequences to the swine industry. control of the disease has been hampered by the unavailability of vaccines. we recently reported the development of two experimental vaccine strains (asfv-g-δ9gl and asfv-g-δmgf) based on the attenuation of the highly virulent and epidemiologically relevant georgia2007 isolate. deletion of the 9gl gene or six genes of the mgf360/505 group produced tw ... | 2016 | 27182007 |
| improved strategy for molecular characterization of african swine fever viruses from sardinia, based on analysis of p30, cd2v and i73r/i329l variable regions. | african swine fever virus (asfv) is the aetiological agent of a highly lethal haemorrhagic disease affecting pigs that inflicts significant economic damage on the swine industry. asf is present in many african countries, in several eastern and central european countries and in sardinia (italy). sequence analyses of variable genomic regions have been extensively used for molecular epidemiological studies of asfv isolates. previous sequencing data of genes that codify for viral protein p54, p72 an ... | 2016 | 27174805 |
| complete genome sequence of a classical swine fever virus isolate belonging to new subgenotype 2.1d from henan province, central china. | we report here the complete genome sequence of hen1505, a field isolate of classical swine fever virus belonging to the new subgenotype 2.1d. hen1505 distinguishes itself from other classical swine fever virus (csfvs) by 1 amino acid substitution in position 159 (threonine by isoleucine), which led to the loss of one n-glycosylation site in the n(pro) protein. | 2016 | 27174260 |
| towards a peptide-based suspension array for the detection of pestivirus antibodies in swine. | classical swine fever (csf) is a highly contagious and lethal disease in swine. serological tests for the diagnosis of csf need not only to detect antibodies against csfv, but also need to differentiate these from antibodies against other pestiviruses. to investigate the possibilities of specific peptide-based serology, various synthetic peptides that represent a well-described linear epitope of the csfv e2 protein (tavspttlr) were used to test the viability of a peptide-based suspension array f ... | 2016 | 27166561 |
| in vitro adaptation and genome analysis of a sub-subgenotype 2.1c isolate of classical swine fever virus. | classical swine fever (csf) still causes substantial economic losses in the pig industry in china. this study reports the isolation and characterization of a field csf virus named gd53/2011 from pig kidney tissue collected during a csf outbreak in guangdong province, china. phylogenetic analysis based on the full-length e2 gene sequence revealed that this isolate belongs to csfv sub-subgenotype 2.1c. to further understand the replication characteristics, gd53/2011 was subsequently adapted in pk- ... | 2016 | 27155669 |
| antiviral role of ifitm proteins in african swine fever virus infection. | the interferon-induced transmembrane (ifitm) protein family is a group of antiviral restriction factors that impair flexibility and inhibit membrane fusion at the plasma or the endosomal membrane, restricting viral progression at entry. while ifitms are widely known to inhibit several single-stranded rna viruses, there are limited reports available regarding their effect in double-stranded dna viruses. in this work, we have analyzed a possible antiviral function of ifitms against a double strand ... | 2016 | 27116236 |
| african swine fever virus serotype-specific proteins are significant protective antigens for african swine fever. | african swine fever (asf) is an emerging disease threat for the swine industry worldwide. no asf vaccine is available and progress is hindered by lack of knowledge concerning the extent of asfv strain diversity and the viral antigens conferring type-specific protective immunity in pigs. available data from vaccination/challenge experiments in pigs indicate that asf protective immunity may be haemadsorption inhibition (hai) serotype-specific. recently, we have shown that two asfv proteins, cd2v ( ... | 2016 | 27114233 |
| safety and immunogenicity of a ge/gi/tk gene-deleted pseudorabies virus variant expressing the e2 protein of classical swine fever virus in pigs. | classical swine fever (csf) and pseudorabies (pr) are both major infectious diseases of pigs, causing enormous economic losses to the swine industry in many countries. a marker vaccine that enables differentiation of infected from vaccinated animals (diva) is highly desirable for control and eradication of these two diseases in endemic areas. since late 2011, pr outbreaks have been frequently reported in many bartha-k61-vaccinated pig farms in china. it has been demonstrated that a pseudorabies ... | 2016 | 27113530 |
| african swine fever virus undergoes outer envelope disruption, capsid disassembly and inner envelope fusion before core release from multivesicular endosomes. | african swine fever virus (asfv) is a nucleocytoplasmic large dna virus (ncldv) that causes a highly lethal disease in domestic pigs. as other ncldvs, the extracellular form of asfv possesses a multilayered structure consisting of a genome-containing nucleoid successively wrapped by a thick protein core shell, an inner lipid membrane, an icosahedral protein capsid and an outer lipid envelope. this structural complexity suggests an intricate mechanism of internalization in order to deliver the vi ... | 2016 | 27110717 |
| recoding structural glycoprotein e2 in classical swine fever virus (csfv) produces complete virus attenuation in swine and protects infected animals against disease. | controlling classical swine fever (csf) mainly involves vaccination with live attenuated vaccines (lav). experimental csfv lavs has been lately developed through reverse genetics using several different approaches. here we present that codon de-optimization in the major csfv structural glycoprotein e2 coding region, causes virus attenuation in swine. four different mutated constructs (pcsfm1-pcsfm4) were designed using various mutational approaches based on the genetic background of the highly v ... | 2016 | 27110709 |
| prokaryotic expression, purification and antigenicity analysis of african swine fever virus pk205r protein. | african swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. the pk205r protein of the african swine fever virus (asfv) is largely expressed in the early stages of infection, which has given the k205r gene extensive attention. in this study, the asfv k205r was cloned and expressed in escherichia coli bl21 (de3). expression of histidine-tagged pk205r with a molecular mass of 44 kda was determined by 12% sodium dodecyl sulfate-polyacrylami ... | 2016 | 27096786 |
| genetic diversity of subgenotype 2.1 isolates of classical swine fever virus. | as the causative agent of classical swine fever, the economically devastating swine disease worldwide, classical swine fever virus (csfv) is currently classified into the 11 subgenotypes, of which subgenotype 2.1 is distributed worldwide and showing more genetic diversity than other subgenotypes. prior to this report, subgenotype 2.1 was divided into three sub-subgenotypes (2.1a-2.1c). to further analyze the genetic diversity of csfv isolates in china, 39 csfv isolates collected between 2004 and ... | 2016 | 27085291 |
| detection system based on magnetoelastic sensor for classical swine fever virus. | in this paper, a magnetoelastic (me) sensing system for the detection of classical swine fever virus (csfv) is presented. the detection system comprises a test paper and a measurement circuit. the test paper consists mainly of an me biosensor to detect the csfv. based on the impedance analysis technique, the measurement circuit is designed to measure the resonance frequency of the me biosensor. the anti-csfv igg is immobilized onto the me sensor surface to form the me biosensor through a physica ... | 2016 | 27078750 |
| development of a novel single-step reverse genetics system for the generation of classical swine fever virus. | we describe an alternative reverse genetics system for generating classical swine fever virus (csfv) based on swine rna polymerase i promoter (pspi)-mediated vrna transcription. the recombinant plasmid pspti/sm harboring a full-length csfv shimen strain cdna, flanked by a swine rna polymerase i (pol i) promoter sequence at the 5' end and a murine pol i terminator sequence at the 3' end, was constructed. when the plasmid pspti/sm was introduced into pk-15 cells by transfection, an infectious csfv ... | 2016 | 27068166 |
| functional characterization and inhibition of the type ii dna topoisomerase coded by african swine fever virus. | dna topoisomerases are essential for dna metabolism and while their role is well studied in prokaryotes and eukaryotes, it is less known for virally-encoded topoisomerases. african swine fever virus (asfv) is a nucleo-cytoplasmic large dna virus that infects ornithodoros ticks and all members of the family suidae, representing a global threat for pig husbandry with no effective vaccine nor treatment. it was recently demonstrated that asfv codes for a type ii topoisomerase, highlighting a possibl ... | 2016 | 27060564 |
| evaluation of specific humoral immune response in pigs vaccinated with cell culture adapted classical swine fever vaccine. | to determine an efficient vaccination schedule on the basis of the humoral immune response of cell culture adapted live classical swine fever virus (csfv) vaccinated pigs and maternally derived antibody (mda) in piglets of vaccinated sows. | 2016 | 27057117 |
| sequence-based comparative study of classical swine fever virus genogroup 2.2 isolate with pestivirus reference strains. | this study was undertaken with the aim to compare and establish the genetic relatedness between classical swine fever virus (csfv) genogroup 2.2 isolate and pestivirus reference strains. | 2015 | 27047198 |
| pathology and molecular diagnosis of classical swine fever in mizoram. | clinical histopathological and molecular diagnosis of classical swine fever disease in pigs of mizoram. | 2015 | 27047001 |
| sensitivity of african swine fever virus to type i interferon is linked to genes within multigene families 360 and 505. | african swine fever virus (asfv) causes a lethal haemorrhagic disease of pigs. there are conflicting reports on the role of interferon in asfv infection. we therefore analysed the interaction of asfv with porcine interferon, in vivo and in vitro. virulent asfv induced biologically active ifn in the circulation of pigs from day 3-post infection, whereas low virulent our t88/3, which lacks genes from multigene family (mgf) 360 and mgf505, did not. infection of porcine leucocytes enriched for dendr ... | 2016 | 27043071 |
| evidence for tunisian-like pestiviruses presence in small ruminants in italy since 2007. | the genus pestivirus, which belongs to the flaviviridae family, includes ssrna+ viruses responsible for infectious diseases in pigs, cattle, sheep, goats and other domestic and wild ruminants. like most of the rna viruses, pestivirus has high genome variability with practical consequences on disease epidemiology, diagnosis and control. in addition to the officially recognized species in the genus pestivirus, such as bvdv-1, bvdv-2, bdv and csfv, other pestiviruses have been detected. furthermore ... | 2016 | 27041547 |
| african swine fever virus introduction into the eu in 2014: experience of latvia. | african swine fever (asf) virus was introduced in latvia in june 2014. thirty-two outbreaks in domestic pigs and 217 cases in wild boar were notified in 2014. twenty-eight outbreaks (87.5%) were primary outbreaks. the contagiosity within pig herds was low. failure to use simple biosecurity measures to reduce the chance of virus introduction, for example by inadvertent feeding of locally produced virus contaminated fodder were the main causes for the outbreaks in backyard holdings. the infection ... | 2016 | 27033903 |
| identification and functional analysis of porcine basic helix-loop-helix transcriptional factor 3 (tcf3) and its alternative splicing isoforms. | the transcription factor 3 (tcf3) is a basic helix-loop-helix transcription factor and is essential for lymphocyte development and epithelial-mesenchymal transition. the splicing isoform, genomic organization and physiological roles of tcf3 have not been elucidated well in pig. based on rna-seq database, four alternative splicing isoforms were identified. splicing isoforms tcf3(e12), tcf3(e47), and tcf3a expressed globally in porcine tissues, but tcf3b mainly expressed in spleen and endoderm der ... | 2016 | 27033898 |
| development of single dilution immunoassay to detect e2 protein specific classical swine fever virus antibody. | classical swine fever virus (csfv) is the causative agent of a highly contagious disease in swine. the disease is endemic in different parts of the world and vaccination is the only way to protect pigs from csfv infection. the virus surface protein e2 is the major immunogenic protein eliciting protective immunity against csfv infection in swine. the whole virus antigen cannot differentiate csfv from other pestiviruses as it cross reacts with border disease and bovine viral diarrhoea viruses. com ... | 2016 | 27032503 |
| what can pestiviral endonucleases teach us about innate immunotolerance? | pestiviruses including bovine viral diarrhea virus (bvdv), border disease virus (bdv) and classical swine fever virus (csfv), occur worldwide and are important pathogens of livestock. a large part of their success can be attributed to the induction of central immunotolerance including b- and t-cells upon fetal infection leading to the generation of persistently infected (pi) animals. in the past few years, it became evident that evasion of innate immunity is a central element to induce and maint ... | 2016 | 27021825 |
| development of cross-priming amplification for direct detection of the african swine fever virus, in pig and wild boar blood and sera samples. | african swine fever (asf) is considered a major threat to the production of pigs worldwide. the asf aetiological agent, asfv, is the sole member of the asfivirus genus, belonging to the asfarviridae family. an effective asf vaccine is not currently available, thus the only measures of asf spread control include, reliable and fast diagnosis. officially approved, diagnostic methods include, virus isolation, serological assays, including enzyme-linked immunosorbent assay and immunoperoxidase assay ... | 2016 | 27002564 |
| recombinant newcastle disease virus expressing african swine fever virus protein 72 is safe and immunogenic in mice. | african swine fever (asf) is a lethal hemorrhagic disease that affects wild and domestic swine. the etiological agent of asf is african swine fever virus (asfv). since the first case was described in kenya in 1921, the disease has spread to many other countries. no commercial vaccines are available to prevent asf. in this study, we generated a recombinant newcastle disease virus (rndv) expressing asfv protein 72 (p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a ... | 2016 | 26980334 |
| transmission routes of african swine fever virus to domestic pigs: current knowledge and future research directions. | african swine fever (asf) is a major threat to the pig industry in europe. since 2007, asf outbreaks have been ongoing in the caucasus, eastern europe and the baltic countries, causing severe economic losses for many pig farmers and pork producers. in addition, the number of asf cases in wild boar populations has dramatically increased over the past few years. evidence supports direct contact with infectious domestic pigs and wild boars, and consumption of contaminated feed, as the main transmis ... | 0 | 26966305 |
| safety of classical swine fever virus vaccine strain lom in pregnant sows and their offspring. | the present study aimed to evaluate the safety of the classical swine fever virus (csfv) vaccine strain lom in pregnant sows. pregnant sows with free csfv antibody were inoculated with a commercial lom vaccine during early pregnancy (day 38; n=3) or mid-pregnancy (days 49-59; n=11). in pregnant sows vaccinated during the early stages of gestation, abortion (day 109) was observed in one case, with two stillbirths and seven mummified fetuses. the viability of live-born piglets was 34.9% in sows va ... | 2016 | 26947495 |
| african swine fever virus: current state and future perspectives in vaccine and antiviral research. | african swine fever (asf) is among the most significant of swine diseases for which no effective vaccines and antivirals are available. the disease, which is endemic in africa, was introduced to trans-caucasian countries and the russian federation in 2007, where it remains prevalent today among domestic pigs and wild boars. although some measures were implemented, asf continues to pose a global risk for all countries, and thereby highlighting the importance of vaccine and antiviral research. in ... | 2016 | 26931386 |
| classical swine fever virus vs. classical swine fever virus: the superinfection exclusion phenomenon in experimentally infected wild boar. | two groups with three wild boars each were used: group a (animals 1 to 3) served as the control, and group b (animals 4 to 6) was postnatally persistently infected with the cat01 strain of csfv (primary virus). the animals, six weeks old and clinically healthy, were inoculated with the virulent strain margarita (secondary virus). for exclusive detection of the margarita strain, a specific qrt-pcr assay was designed, which proved not to have cross-reactivity with the cat01 strain. the wild boars ... | 2016 | 26919741 |
| a promising trigene recombinant human adenovirus vaccine against classical swine fever virus. | classical swine fever (csf) vaccine based on hadv-5 had achieved an efficient protection in swine. both classical swine fever virus (csfv) e0 glycoprotein and e2 glycoprotein were the targets for neutralizing antibodies and related to immune protection against csf. interleukin-2 (il2), as an adjuvant, also had been used in csf vaccine research. in this study, coexpression of the csfv e0, e2, and il2 genes by hadv-5 (radv-e0-e2-il2) was constructed and immunized to evaluate its efficacy. three ex ... | 2016 | 26918463 |
| domain organization of vaccinia virus helicase-primase d5. | poxviridae are viruses with a large linear double-stranded dna genome coding for up to 250 open reading frames and a fully cytoplasmic replication. the double-stranded dna genome is covalently circularized at both ends. similar structures of covalently linked extremities of the linear dna genome are found in the african swine fever virus (asfarvirus) and in the phycodnaviridae we are studying the machinery which replicates this peculiar genome structure. from our work with vaccinia virus, we giv ... | 2016 | 26912611 |
| different evolutionary patterns of classical swine fever virus envelope proteins. | classical swine fever virus (csfv) is the causative agent of classical swine fever, which is a highly contagious disease of the domestic pig as well as wild boar. the proteins e(rns), e1, and e2 are components of the viral envelope membrane. they are also implicated in virus attachment and entry, replication, and (or) anti-immune response. here, we studied the genetic variations of these envelope proteins in the evolution of csfv. the results reveal that the envelope proteins underwent different ... | 2016 | 26911308 |
| genetic diversity and positive selection analysis of classical swine fever virus envelope protein gene e2 in east china under c-strain vaccination. | classical swine fever virus (csfv) causes an economically important and highly contagious disease of pigs worldwide. c-strain vaccination is one of the most effective ways to contain this disease. since 2014, sporadic csf outbreaks have been occurring in some c-strain vaccinated provinces of china. to decipher the disease etiology, 25 csfv e2 genes from 169 clinical samples were cloned and sequenced. phylogenetic analyses revealed that all 25 isolates belonged to subgenotype 2.1. twenty-three of ... | 2016 | 26903966 |
| guanylate-binding protein 1, an interferon-induced gtpase, exerts an antiviral activity against classical swine fever virus depending on its gtpase activity. | many viruses trigger the type i interferon (ifn) pathway upon infection, resulting in the transcription of hundreds of interferon-stimulated genes (isgs), which define the antiviral state of the host. classical swine fever virus (csfv) is the causative agent of classical swine fever (csf), a highly contagious viral disease endangering the pig industry in many countries. however, anti-csfv isgs are poorly documented. here we screened 20 isgs that are commonly induced by type i ifns against csfv i ... | 2016 | 26889038 |
| rna interference screening of interferon-stimulated genes with antiviral activities against classical swine fever virus using a reporter virus. | classical swine fever (csf) caused by classical swine fever virus (csfv) is a highly contagious and often fatal disease of pigs, which leads to significant economic losses in many countries. viral infection can induce the production of interferons (ifns), giving rise to the transcription of hundreds of ifn-stimulated genes (isgs) to exert antiviral effects. although numerous isgs have been identified to possess antiviral activities against different viruses, rare anti-csfv isgs have been reporte ... | 2016 | 26868874 |
| assessment of the geographic distribution of ornithodoros turicata (argasidae): climate variation and host diversity. | ornithodoros turicata is a veterinary and medically important argasid tick that is recognized as a vector of the relapsing fever spirochete borrelia turicatae and african swine fever virus. historic collections of o. turicata have been recorded from latin america to the southern united states. however, the geographic distribution of this vector is poorly understood in relation to environmental variables, their hosts, and consequently the pathogens they transmit. | 2016 | 26829327 |
| effect of o. porcinus tick salivary gland extract on the african swine fever virus infection in domestic pig. | african swine fever is a haemorrhagic disease in pig production that can have disastrous financial consequences for farming. no vaccines are currently available and animal slaughtering or area zoning to restrict risk-related movements are the only effective measures to prevent the spread of the disease. ornithodoros soft ticks are known to transmit the african swine fever virus (asfv) to pigs in farms, following the natural epidemiologic cycle of the virus. tick saliva has been shown to modulate ... | 2016 | 26828597 |
| complete genome sequence analysis of acute and mild strains of classical swine fever virus subgenotype 3.2. | we report the complete genome sequences of two classical swine fever virus strains (jj9811 and yi9908). both belong to subgenotype 3.2. strain jj9811 causes mild symptoms and strain yi9908 causes acute symptoms. the sequences were 95.7% homologous at the nucleotide level and 95.6% homologous at the amino acid level. | 2016 | 26823570 |
| a multiplex pcr for simultaneous detection of classical swine fever virus, african swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines. | in this assay, we developed and evaluated a multiplex pcr (mpcr) for its ability in detecting multiple infections of swine simultaneously. four pairs of primers were used to detect five viruses. specific primers were designed for classical swine fever virus (csfv), african swine fever virus (asfv) and pseudorabies (prv). a pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respirato ... | 2015 | 26812812 |
| fkbp8 interact with classical swine fever virus ns5a protein and promote virus rna replication. | the non-structural 5a (ns5a) protein of classical swine fever virus (csfv) is proven to be involved in viral replication and can also modulate cellular signaling and host cellular responses via to its ability to interact with various cellular proteins. fkbp8 is also reported to promote virus replication. here, we show that ns5a specifically interacts with fkbp8 through coimmunoprecipitation and gst-pulldown studies. additionally, confocal microscopy study showed that ns5a and fkbp8 colocalized i ... | 2016 | 26748656 |
| [research progress in the core proteins of the classical swine fever virus]. | the core protein (cp) of the classical swine fever virus (csfv) is one of its structural proteins. apart from forming the nucleocapsid to protect internal viral genomic rna, this protein is involved in transcriptional regulation. also, during viral infection, the cp is involved in interactions with many host proteins. in this review, we combine study of this protein with its disorders, structural/functional characteristics, as well as its interactions with the non-structural proteins ns3, ns5b a ... | 2015 | 26738299 |
| african swine fever virus transmission cycles in central europe: evaluation of wild boar-soft tick contacts through detection of antibodies against ornithodoros erraticus saliva antigen. | african swine fever (asf) is one of the most complex viral diseases affecting both domestic and wild pigs. it is caused by asf virus (asfv), the only dna virus which can be efficiently transmitted by an arthropod vector, soft ticks of the genus ornithodoros. these ticks can be part of asfv-transmission cycles, and in europe, o. erraticus was shown to be responsible for long-term maintenance of asfv in spain and portugal. in 2014, the disease has been reintroduced into the european union, affecti ... | 2016 | 26728767 |
| simultaneous typing of seven porcine pathogens by multiplex pcr with a gexp analyser. | a novel high-throughput method was developed for simultaneous detection and differentiation of seven porcine pathogens by multiplex pcr based on a genomelab gene expression profiler (gexp) analyser. the pathogens included in this study were pseudorabies virus (prv), classical swine fever virus (csfv), african swine fever virus (asfv), porcine reproductive and respiratory syndrome virus (prrsv), porcine parvovirus (ppv), porcine circovirus type 2 (pcv-2) and japanese encephalitis virus (jev). sev ... | 2016 | 26706731 |
| [classical swine fever in wild boar in rhineland-palatinate: evaluation of the official control measures from 2005-2011]. | the present study describes the control strategy for fighting classical swine fever in wild boar in rhineland-palatinate from 2005 to 2011 and evaluates its effectiveness. the official control measures were based on the following three main pillars:--serological and virological monitoring: by means of serological monitoring classical swine fever outbreaks could be detected very early. increasing antibody prevalences indicated an imminent classical swine fever outbreak. this could be confirmed by ... | 2015 | 26697717 |
| evolution and molecular epidemiology of classical swine fever virus during a multi-annual outbreak amongst european wild boar. | classical swine fever is a viral disease of pigs that carries tremendous socio-economic impact. in outbreak situations, genetic typing is carried out for the purpose of molecular epidemiology in both domestic pigs and wild boar. these analyses are usually based on harmonized partial sequences. however, for high-resolution analyses towards the understanding of genetic variability and virus evolution, full-genome sequences are more appropriate. in this study, a unique set of representative virus s ... | 2016 | 26684209 |
| a recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus. | recently, porcine deltacoronavirus (pdcov) has been proven to be associated with enteric disease in piglets. diagnostic tools for serological surveys of pdcov remain in the developmental stage when compared with those for other porcine coronaviruses. in our study, an indirect enzyme-linked immunosorbent assay (elisa) (rpdcov-n-elisa) was developed to detect antibodies against pdcov using a histidine-tagged recombinant nucleocapsid (n) protein as an antigen. the rpdcov-n-elisa did not cross-react ... | 2016 | 26668175 |
| immune responses against classical swine fever virus: between ignorance and lunacy. | classical swine fever virus infection of pigs causes disease courses from life-threatening to asymptomatic, depending on the virulence of the virus strain and the immunocompetence of the host. the virus targets immune cells, which are central in orchestrating innate and adaptive immune responses such as macrophages and conventional and plasmacytoid dendritic cells. here, we review current knowledge and concepts aiming to explain the immunopathogenesis of the disease at both the host and the cell ... | 2015 | 26664939 |
| deletion of the thymidine kinase gene induces complete attenuation of the georgia isolate of african swine fever virus. | african swine fever virus (asfv) is the etiological agent of a contagious and often lethal viral disease of domestic pigs. there are no vaccines to control africa swine fever (asf). experimental vaccines have been developed using genetically modified live attenuated asfvs obtained by specifically deleting virus genes involved in virulence, including the thymidine kinase (tk) gene. tk has been shown to be involved in the virulence of several viruses, including asfv. here we report the constructio ... | 2016 | 26656424 |
| molecular typing and phylogenetic analysis of classical swine fever virus isolates from kerala, india. | classical swine fever (csf) is an economically important disease of pigs caused by csf virus (csfv) belonging to the genus pestivirus within the family flaviviridae. the disease is endemic in many countries including india. a comprehensive study was carried out to assess the type of csfv circulating in the south indian state of kerala. during the period 2013-2014, clinical samples were collected from 19 suspected csf outbreaks of domestic pigs in different districts of kerala. the samples were t ... | 2015 | 26645036 |
| genome sequence of african swine fever virus ba71, the virulent parental strain of the nonpathogenic and tissue-culture adapted ba71v. | the strain ba71v has played a key role in african swine fever virus (asfv) research. it was the first genome sequenced, and remains the only genome completely determined. a large part of the studies on the function of asfv genes, viral transcription, replication, dna repair and morphogenesis, has been performed using this model. this avirulent strain was obtained by adaptation to grow in vero cells of the highly virulent ba71 strain. we report here the analysis of the genome sequence of ba71 in ... | 2015 | 26618713 |
| generation and evaluation of a chimeric classical swine fever virus expressing a visible marker gene. | classical swine fever virus (csfv) is a noncytopathogenic virus, and the incorporation of an enhanced green fluorescent protein (egfp) tag into the viral genome provides a means of direct monitoring of viral infection without immunostaining. it is well established that the 3' untranslated region (3'-utr) of the csfv plays an important role in viral rna replication. although csfv carrying a reporter gene and chimeric csfv have been generated and evaluated, a chimeric csfv with a visible marker ha ... | 2016 | 26614259 |
| small-scale pig farmers' behavior, silent release of african swine fever virus and consequences for disease spread. | the expanding distribution of african swine fever (asf) is threatening the pig industry worldwide. most outbreaks occur in backyard and small-scale herds, where poor farmers often attempt to limit the disease's economic consequences by the emergency sale of their pigs. the risk of african swine fever virus (asfv) release via this emergency sale was investigated. simulation modeling was used to study asfv transmission in backyard and small-scale farms as well as the emergency sale of pigs, and th ... | 2015 | 26610850 |
| cholesterol flux is required for endosomal progression of african swine fever virions during the initial establishment of infection. | african swine fever virus (asfv) is a major threat for porcine production that has been slowly spreading in eastern europe since its first appearance in the caucasus in 2007. asfv enters the cell by endocytosis and gains access to the cytosol to start replication from late endosomes and multivesicular bodies. cholesterol associated with low-density lipoproteins entering the cell by endocytosis also follows a trafficking pathway similar to that of asfv. here we show that cholesterol plays an esse ... | 2015 | 26608317 |
| live attenuated african swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection. | african swine fever virus (asfv) is the causal agent of african swine fever, a hemorrhagic and often lethal porcine disease causing enormous economical losses in affected countries. endemic for decades in most of the sub-saharan countries and sardinia, the risk of asfv-endemicity in europe has increased since its last introduction into europe in 2007. live attenuated viruses have been demonstrated to induce very efficient protective immune responses, albeit most of the time protection was circum ... | 2015 | 26589145 |
| [hsp70 fused with the envelope glycoprotein e0 of classical swine fever virus enhances immune responses in balb/c mice]. | heat-shock protein (hsp) 70 potentiates specific immune responses to some antigenic peptides fused to it. here, the prokaryotic plasmids harboring the envelope glycoprotein e0 gene of classical swine fever virus (csfv) and/or the hsp70 gene of haemophilus parasuis were constructed and expressed in escherichia coli rosseta 2(r2). the fusion proteins were then purified. groups of balb/c mice were immunized with these fusion proteins, respectively, and sera collected 7 days after the third immuniza ... | 2015 | 26524908 |
| current status of african swine fever virus in a population of wild boar in eastern poland (2014-2015). | african swine fever virus (asfv) was detected in wild boar in eastern poland in early 2014. so far, 65 cases of asfv infection in wild boar have been recognised. the methods used for asfv detection included highly specific real-time pcr with a universal probe library (upl), enzyme-linked immunosorbent assay (elisa), and an immunoperoxidase test (ipt) for identification of anti-asfv antibodies. the positive asf cases were located near the border with belarus in sokółka and białystok counties. som ... | 2015 | 26497350 |
| creation of functional viruses from non-functional cdna clones obtained from an rna virus population by the use of ancestral reconstruction. | rna viruses have the highest known mutation rates. consequently it is likely that a high proportion of individual rna virus genomes, isolated from an infected host, will contain lethal mutations and be non-functional. this is problematic if the aim is to clone and investigate high-fitness, functional cdnas and may also pose problems for sequence-based analysis of viral evolution. to address these challenges we have performed a study of the evolution of classical swine fever virus (csfv) using de ... | 2015 | 26485566 |
| episodic adaptive diversification of classical swine fever virus rna-dependent rna polymerase ns5b. | classical swine fever virus (csfv) is the pathogen that causes a highly infectious disease of pigs and has led to disastrous losses to pig farms and related industries. the rna-dependent rna polymerase (rdrp) ns5b is a central component of the replicase complex (rc) in some single-stranded rna viruses, including csfv. on the basis of genetic variation, the csfv rdrps could be clearly divided into 2 major groups and a minor group, which is consistent with the phylogenetic relationships and virule ... | 2015 | 26485449 |
| construction of infectious cdna clone derived from a classical swine fever virus field isolate in bac vector using in vitro overlap extension pcr and recombination. | to develop reverse genetics system of rna viruses, cloning of full-length viral genome is required which is often challenging due to many steps involved. in this study, we report cloning of full-length cdna from an indian field isolate (csfv/ivri/vb-131) of classical swine fever virus (csfv) using in vitro overlap extension pcr and recombination which drastically reduced the number of cloning steps. the genome of csfv was amplified in six overlapping cdna fragments, linked by overlap extension p ... | 2015 | 26478540 |
| porcine mx1 fused to hiv tat protein transduction domain (ptd) inhibits classical swine fever virus infection in vitro and in vivo. | classical swine fever (csf) caused by csf virus (csfv) is highly contagious andcauses significant economic losses in the pig industry throughout the world. previously we demonstrated that porcine mx1 (pomx1), when fused to hiv tat protein transduction domain (ptd), inhibits csfv propagation in pk-15 cells, but it is unknown whether ptd-pomx1 exhibits antiviral activity in other porcine lines and it is efficacious for controlling csfv infection in pigs in china. | 2015 | 26472464 |
| geographic range of vector-borne infections and their vectors: the role of african wildlife. | the role of african wildlife in the occurrence of vector-borne infections in domestic animals has gained renewed interest as emerging and re-emerging infections occur worldwide at an increasing rate. in africa, biodiversity conservation and the expansion of livestock production have increased the risk of transmitting vector-borne infections between wildlife and livestock. the indigenous african pathogens with transboundary potential, such as rift valley fever virus, african horse sickness virus, ... | 2015 | 26470454 |
| ion channel activity of the csfv p7 viroporin in surrogates of the er lipid bilayer. | viroporins comprise a family of non-structural proteins that play significant and diverse roles during the replication cycle of many animal viruses. consequently, they have become promising targets for inhibitory drug and vaccine development. structure–function traits common to all members of the family are their small size (ca. 60–120 aa), high hydrophobicity, and the presence of helical domains that transverse the membrane and assemble into oligomeric-permeating structures therein. the possibi ... | 2016 | 26464198 |
| midgut proteome of an argasid tick, ornithodoros erraticus: a comparison between unfed and engorged females. | the argasid tick ornithodoros erraticus is the vector of african swine fever virus and of several borrelia species that cause human relapsing fever in the iberian peninsula. the tick midgut is part of the ectoparasite-host interface and expresses proteins that are vital for the survival of the tick. midgut proteins are therefore potential targets for drug and/or vaccine design aimed at the development of new strategies for tick control. thus, the aim of this work was the characterization of the ... | 2015 | 26459090 |
| identification of two amino acids within e2 important for the pathogenicity of chimeric classical swine fever virus. | our previous study demonstrated that a chimeric classical swine fever virus (csfv) vsm/ce2 containing the e2 gene of the vaccine c-strain on the genetic background of the virulent csfv strain shimen (vsm) was attenuated in swine but reversed to virulence after serial passages in pk15 cells. to investigate the molecular basis of the pathogenicity, the genome of the 11th passage vsm/ce2 variant (vsm/ce2-p11) was sequenced, and two amino acid mutations, t745i and m979k, within e2 of vsm/ce2-p11 wer ... | 2016 | 26454191 |
| characterization of cytopathogenicity of classical swine fever virus isolate induced by newcastle disease virus. | classical swine fever virus (csfv), the causative agent of classical swine fever, belongs to the family flaviviridae and genus pestivirus. some pestiviruses exhibit cytopathic effect in cell culture but exact phenomenon is unknown. over expression of ns2-3 gene, presence of defective interfering particle and exaltation of newcastle disease virus (end) phenomenon could be the reasons of cytopathogenicity. in the present study, a csfv isolate exhibiting cytopathic effect (cpe) in madin-darby canin ... | 2015 | 26436124 |
| an investigation of classical swine fever virus seroprevalence and risk factors in pigs in timor-leste. | classical swine fever virus (csfv) is a highly infectious pathogen of pigs and believed to be a major constraint to pig production in timor-leste. the ministry of agriculture and fisheries conducts vaccination campaigns in an attempt to control clinical disease, however, there is no empirical data available concerning the seroprevalence and distribution of csfv in timor-leste. to help address this knowledge deficit, a cross-sectional study to determine seroprevalence was conducted in the three d ... | 2015 | 26433742 |
| first detection of antibodies against african swine fever virus in faeces samples. | african swine fever (asf) is a viral, highly lethal haemorrhagic disease of swine with no available vaccine or effective treatment. introduction of asf into a country triggers immediate restriction measures that cause significant economic losses and threatens spread to neighbouring countries. wild boar populations have been recently assigned an essential role in the spread of african swine fever virus (asfv) to european countries. therefore, effective surveillance and monitoring of wild boar pop ... | 2015 | 26431943 |
| in vitro coinfection and replication of classical swine fever virus and porcine circovirus type 2 in pk15 cells. | increasing clinical lines of evidence have shown the coinfection/superinfection of porcine circovirus type 2 (pcv2) and classical swine fever virus (csfv). here, we investigated whether pcv2 and csfv could infect the same cell productively by constructing an in vitro coinfection model. our results indicated that pcv2-free pk15 cells but not st cells were more sensitive to pcv2, and the pk15 cell line could stably harbor replicating csfv (pk15-csfv cells) with a high infection rate. confocal and ... | 2015 | 26431319 |
| genetic clustering of recent classical swine fever virus isolates from karnataka, india revealed the emergence of subtype 2.2 replacing subtype 1.1. | the phylogenetic analysis of 11 csfv isolates from karnataka, india obtained during the year 2012-13 was undertaken to obtain the most reliable genetic typing of the csfv isolates based on e2, ns5b and 5'utr genomic regions. the study indicated that all the 11 csfv isolates belonged to subgroup 2.2. the most reliable classification was obtained with sequence data from the ns5b region which separated all the isolates based on the history of outbreak and geographic origin. analysis of full length ... | 2015 | 26396984 |
| classical swine fever virus marker vaccine strain cp7_e2alf: genetic stability in vitro and in vivo. | recently, cp7_e2alf (suvaxyncsf marker), a live marker vaccine against classical swine fever virus, was licensed through the european medicines agency. for application of such a genetically engineered virus under field conditions, knowledge about its genetic stability is essential. here, we report on stability studies that were conducted to assess and compare the mutation rate of cp7_e2alf in vitro and in vivo. sequence analyses upon passaging confirmed the high stability of cp7_e2alf, and no re ... | 2015 | 26392285 |
| alterations of nuclear architecture and epigenetic signatures during african swine fever virus infection. | viral interactions with host nucleus have been thoroughly studied, clarifying molecular mechanisms and providing new antiviral targets. considering that african swine fever virus (asfv) intranuclear phase of infection is poorly understood, viral interplay with subnuclear domains and chromatin architecture were addressed. nuclear speckles, cajal bodies, and promyelocytic leukaemia nuclear bodies (pml-nbs) were evaluated by immunofluorescence microscopy and western blot. further, efficient pml pro ... | 2015 | 26389938 |
| experimental infection of ornithodoros erraticus sensu stricto with two portuguese african swine fever virus strains. study of factors involved in the dynamics of infection in ticks. | african swine fever (asf) is a frequently devastating hemorrhagic disease of domestic pigs and wild boar and ornithodoros erraticus sensu stricto argasid ticks are the only biological vectors of african swine fever virus (asfv) known to occur in europe. recently this disease emerged in eastern europe and russian federation, showing a huge potential for a rapid spread between countries. there is some risk of re-emergence of asf in the countries where these ticks exist, that can contribute for the ... | 2015 | 26366570 |
| generation and efficacy evaluation of a recombinant pseudorabies virus variant expressing the e2 protein of classical swine fever virus in pigs. | classical swine fever (csf) is an economically important infectious disease of pigs caused by classical swine fever virus (csfv). pseudorabies (pr), which is caused by pseudorabies virus (prv), is another important infectious disease of pigs and other animals. coinfections of pigs with prv and csfv occur occasionally in the field. the modified live vaccine bartha-k61 strain has played an important role in the control of pr in many countries, including china. since late 2011, however, increasing ... | 2015 | 26311244 |
| first molecular assessment of the african swine fever virus status of ornithodoros ticks from swaziland. | african swine fever (asf) is an economically significant haemorrhagic disease of domestic pigs. it is caused by the african swine fever virus (asfv), a deoxyribonucleic acid (dna)arbovirus. argasid ticks of the genus ornithodoros, which are widely distributed throughout southern africa, play a primary role in virus maintenance and spread within the endemic sylvatic cycle. the asf status of swaziland is unknown, but this land-locked country is surrounded by asf-positive countries, has a burgeonin ... | 2014 | 26304186 |
| genotyping of classical swine fever virus using high-resolution melt analysis. | discrimination between different field and vaccine strains of classical swine fever virus (csfv) is crucial for meaningful disease diagnosis and epidemiological investigation. in this study, a rapid method for differentiating vaccine strains and outbreak csfv isolates by combined rt-pcr and high-resolution melt (hrm) analysis has been developed. the assay is based on pcr amplification of short fragments from the most variable region of csfvgene e2, followed by hrm analysis of amplicons. real-tim ... | 2015 | 26300371 |
| complete genome sequence of classical swine fever virus strain jszl, belonging to a new subgenotype, 2.1d, isolated in china in 2014. | the complete genome sequence of classic swine fever virus (csfv) strain jszl was determined in this study. jszl was originally isolated from an immune pig farm in jiangsu province, china. jszl is more closely related to subgenotype 2.1b than to 2.1a and 2.1c. importantly, jszl was classified into a new subgenotype, 2.1d. | 2015 | 26294620 |
| eef1a interacts with the ns5a protein and inhibits the growth of classical swine fever virus. | the ns5a protein of classical swine fever virus (csfv) is involved in the rna synthesis and viral replication. however, the ns5a-interacting cellular proteins engaged in the csfv replication are poorly defined. using yeast two-hybrid screen, the eukaryotic elongation factor 1a (eef1a) was identified to be an ns5a-binding partner. the ns5a-eef1a interaction was confirmed by coimmunoprecipitation, glutathione s-transferase (gst) pulldown and laser confocal microscopy assays. the domain i of eef1a ... | 2015 | 26266418 |
| analysis of ttsuv1b antibody in porcine serum and its correlation with four antibodies against common viral infectious diseases. | the purpose of the present study was to evaluate the correlation between torque teno sus virus 1b (ttsuv1b) infection and other viral infections or vaccine immunization in conventional pigs. | 2015 | 26260234 |
| simultaneous detection of eight swine reproductive and respiratory pathogens using a novel gexp analyser-based multiplex pcr assay. | a new high-throughput genomelab gene expression profiler (gexp) analyser-based multiplex pcr assay was developed for the detection of eight reproductive and respiratory pathogens in swine. the reproductive and respiratory pathogens include north american porcine reproductive and respiratory syndrome virus (prrsv-na), classical swine fever virus (csfv), porcine circovirus 2 (pcv-2), swine influenza virus (siv) (including h1 and h3 subtypes), porcine parvovirus (ppv), pseudorabies virus (prv) and ... | 2015 | 26259690 |