Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| survival of ascaris eggs and hygienic quality of human excreta in vietnamese composting latrines. | for centuries farmers in vietnam have fertilized their fields with human excreta collected directly from their household latrines. contrary to the official guideline of six-month storage, the households usually only store human excreta for three to four months before use, since this is the length of time that farmers have available to produce fertilizer between two cropping seasons. this study aimed to investigate whether hygienically safe fertilizer could be produced in the latrines within this ... | 2009 | 20003550 |
| anthelmintic effects of phytogenic feed additives in ascaris suum inoculated pigs. | two experiments were performed to determine the anthelmintic effect of some phytogenic feed additives on a mild infection of ascaris suum in growing and finishing pigs. usually, an infection of a. suum is controlled by using conventional synthetic drugs. organic farmers, however, prefer a non-pharmaceutical approach to worm control. therefore, phytotherapy could be an appropriate alternative. in the first experiment, a commercial available organic starter diet was supplemented with 3% of a herb ... | 2010 | 19954891 |
| sanitation composting process in different seasons. ascaris suum as model. | the influence of aerobic composting of organic wastes (straw, sawdust, wood brash and sludge from wwtp) on the viability of the model parasite eggs were studied in production conditions of the industrial composting plant topvar, inc. in topolcany, slovak republic. during summer and winter seasons (2005-2006), the survival of non-embryonated ascaris suum eggs was evaluated with respect to aerobic composting processes and air temperature, which varied from -6 degrees c to +12 degrees c in winter a ... | 2010 | 19932605 |
| prevalence of nematodes in danish industrialized sow farms with loose housed sows in dynamic groups. | the aim of this study was to investigate the prevalence of nematodes in sows and gilts in modern pig herds which practiced loose housing of sows and gilts. seventy hundred and ninety fecal samples were examined from seventy-nine farms. oesophagostomum spp. was found in 15% of farms and ascaris suum was found in 76% of the farms. trichuris suis was found in a very few instances. no other nematodes were found. the prevalence of oesophagostomum spp. within an infected farm was approximately 50% and ... | 2010 | 19897307 |
| nematicidal activity of two monoterpenoids and ser-2 tyramine receptor of caenorhabditis elegans. | in vitro cultures of two nematodes (caenorhabditis elegans and ascaris suum) were established to study the nematicidal activity of three monoterpenoids (thymol, carvacrol and p-cymene). toxicity of thymol and carvacrol was found for the two nematodes tested. the study was then aimed to address whether nematode tyramine receptor (tyrr) could interact with the two compounds by using hek293 mammalian cells transfected with a c. elegans tyrr (ser-2) sequence, in hope of developing a high-throughput ... | 2010 | 19896925 |
| temperature and deactivation of microbial faecal indicators during small scale co-composting of faecal matter. | small scale co-composting of faecal matter from dry toilet systems with shredded plant material and food waste was investigated in respect to heat development and deactivation of faecal indicators under tropical semiarid conditions. open (uncovered) co-composting of faecal matter with shredded plant material alone did not generate temperatures high enough (<55 degrees c) to reduce the indicators sufficiently. the addition of food waste and confinement in chambers, built of concrete bricks and wo ... | 2010 | 19889525 |
| investigation of intestinal parasites in pig feces that are also human pathogens. | a total of 238 pig fecal specimens were collected from pig farms in corlu (tekirdağ), ayazma, and arnavutköy (istanbul) during the summer. out of the 238 pig specimens, 105 were from pigs younger than 6 months and 133 from pigs older than 6 months. these were investigated for intestine parasites in particular the ones that are human pathogens. cryptosporidium spp. was detected in 21 fecal specimens (8.8%), giardia spp. in 9 (3.7%), balantidium coli cysts in 4 (1.6%) and ascaris suum eggs in 9 (4 ... | 2009 | 19851968 |
| incentive mechanisms for liver lesion control in finishing pigs in the netherlands. | liver lesion prevalence in slaughtered finishing pigs in the netherlands remained relatively high from the mid-1990s until 2004, although sufficient measures existed to control the main cause, an infection with the roundworm ascaris suum. in july 2004 a new incentive mechanism was installed to induce finishing pig producers to increase control of a. suum infections. this paper compares the effectiveness of two dutch incentive mechanisms: a collective insurance - in place prior to july 2004 - and ... | 2010 | 19819576 |
| inhaled vs subcutaneous effects of a dual il-4/il-13 antagonist in a monkey model of asthma. | pitrakinra is a recombinant protein derived from human interleukin-4 (il-4) that binds to il-4ralpha and acts as a competitive antagonist of il-4 and il-13. the studies reported here compare the dose-ranging effects of pitrakinra on allergen-induced airway hyperresponsiveness (ahr) and airway eosinophilia when administered subcutaneously (s.c.) or by inhalation to the ascaris suum-sensitive cynomolgus monkey for the purpose of elucidating the primary site of pitrakinra's anti-asthmatic action. | 2010 | 19796211 |
| genetic influence on the kinetics and associated pathology of the early stage (intestinal-hepatic) migration of ascaris suum in mice. | the generative mechanism(s) of aggregation and predisposition to ascaris lumbricoides and a. suum infections in their host population are currently unknown and difficult to elucidate in humans and pigs for ethical/logistical reasons. a recently developed, optimized murine model based on 2 inbred strains, putatively susceptible (c57bl/6j) and resistant (cba/ca) to infection, was exploited to elucidate further the basis of the contrasting parasite burdens, most evident at the pulmonary stage. we e ... | 2010 | 19765333 |
| a putative nuclear growth factor-like globular nematode specific protein. | expressed sequence tags (ests) are an effective approach for discovery of novel genes. in the current study, approximately 250 ests of the cattle parasitic nematode setaria digitata were examined and a cdna clone identified whose coding sequence could not be functionally annotated by searching over publicly available genome, protein, est and sts databases. here, we report the extensive characterization of this orf (up) and its homologues using a bioinformatic approach. uncharacterized protein (s ... | 2009 | 19759810 |
| origin and evolution of the unique tetra-domain hemoglobin from the hydrothermal vent scale worm branchipolynoe. | hemoglobin is the most common respiratory pigment in annelids. it can be intra or extracellular, and this latter type can form large multimeric complexes. the hydrothermal vent scale worms branchipolynoe symmytilida and branchipolynoe seepensis express an extracellular tetra-domain hemoglobin (hb) that is unique in annelids. we sequenced the gene for the single-domain and tetra-domain globins in these two species. the single-domain gene codes for a mature protein of 137 amino acids, and the tetr ... | 2010 | 19755665 |
| induction of dominant lethal mutations by ascaris trypsin inhibitor in male mice. | trypsin inhibitor (ati) isolated from ascaris suum, a gastrointestinal nematode parasite, was tested for the induction of dominant lethal mutations in male mice. dominant lethal effects of ati for the main stages of germ cell development were analyzed by mating at specific time points after dosing. three groups of adult balb/c males received 50, 100 or 250mg/kg body weight (bw) single intraperitoneal (ip) injection of ati in sterile phosphate-buffered saline (pbs). the control group received con ... | 2010 | 19737609 |
| cpg-containing immunostimulatory dna sequences elicit tnf-alpha-dependent toxicity in rodents but not in humans. | cpg-containing immunostimulatory dna sequences (iss), which signal through tlr9, are being developed as a therapy for allergic indications and have proven to be safe and well tolerated in humans when administrated via the pulmonary route. in contrast, iss inhalation has unexplained toxicity in rodents, which express tlr9 in monocyte/macrophage lineage cells as well as in plasmacytoid dcs (pdcs) and b cells, the principal tlr9-expressing cells in humans. we therefore investigated the mechanisms u ... | 2009 | 19726873 |
| crystallization of mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode ascaris suum with the specific inhibitor flutolanil. | in adult ascaris suum (roundworm) mitochondrial membrane-bound complex ii acts as a rhodoquinol-fumarate reductase, which is the reverse reaction to that of mammalian complex ii (succinate-ubiquinone reductase). the adult a. suum rhodoquinol-fumarate reductase was crystallized in the presence of octaethyleneglycol monododecyl ether and n-dodecyl-beta-d-maltopyranoside in a 3:2 weight ratio. the crystals belonged to the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters a = 123.75, ... | 2009 | 19724139 |
| coprological survey of parasitic infections in pigs and cattle in slaughterhouse in osaka, japan. | a coprological survey was performed at a slaughterhouse in osaka, japan, from 2004 to 2007 on 129 pigs reared in 8 prefectures, and on 213 cattle reared in 21 prefectures. eimeria spp., trichuris suis, ascaris suum and metastrongylus spp. infections were found in 52 (40.3%), 32 (24.8%), 19 (14.7%) and 3 pigs (2.3%), respectively, while eimeria spp., capillaria bovis and trichuris sp. infections were detected in 163 (76.5%), 15 (7.0%) and 8 cattle (3.8%), respectively. our results suggest that en ... | 2009 | 19721362 |
| functional response to inhaled salbutamol and/or ipratropium bromide in ascaris suum-sensitised cats with allergen-induced bronchospasms. | knowledge about the use of inhaled bronchodilators in cats with so-called 'feline asthma' is limited and relies on the experience of clinicians treating these patients. a randomised controlled four-way crossover study was therefore designed to compare the effects of salbutamol (sal, 100 μg), ipratropium bromide (ib, 20 μg) and a combination of both (sal/ib, 100 μg/20 μg), delivered through a pressurised metered-dose inhaler (pmdi) connected to a spacing chamber, on allergen-induced bronchospasms ... | 2010 | 19699123 |
| boosting the suppressive effects of ascaris suum components in ifn-gamma-deficient mice. | high molecular weight components from ascaris suum extract suppress ovalbumin-specific immunity in mice. in ifn-gamma-deficient mice, ovalbumin-specific delayed-type hypersensitivity reactions are more strongly downregulated by these suppressive components. here, the cellularity of the delayed-type hypersensitivity reaction in ifn-gamma-deficient mice and the increased downregulation induced by ascaris suum components were analyzed. il-12p40-dependent neutrophilic influx was predominant. subopti ... | 2009 | 19684988 |
| population dynamics of ascaris suum in trickle-infected pigs. | the population dynamics of ascaris suum was studied by long-term exposure of pigs to infective eggs. the pigs were experimentally inoculated with 25 a. suum eggs/kg/day, and 7, 8, and 8 pigs were necropsied at weeks 4, 8, and 14 postinoculation (pi), respectively. despite the fact that the pigs were continuously reinfected, dramatic reductions in numbers of liver lesions (white spots) and migrating lung larvae were observed as a function of time. however, even at the end of the study, a few larv ... | 2009 | 19673589 |
| production of ascaris suum as14 protein and its fusion protein with cholera toxin b subunit in rice seeds. | plants are attractive vaccine production and oral delivery systems. cereals are excellent candidate for edible vaccines, which can express and store high levels of proteins for extended periods of time without degradation. in this study, we produced a 14-kda protective surface antigen of ascaris suum l3 larvae and its fusion chimera with a mucosal carrier molecule cholera toxin b subunit (ctb) in rice (oryza sativa l.) under the control of the endosperm-specific glutelin-b promoter. we found tha ... | 2009 | 19652493 |
| prevalence of antibodies against ascaris suum and its association with allergic manifestations in 4-year-old children in the netherlands: the piama birth cohort study. | the association between helminth infections and childhood atopic diseases remains controversial. the majority of studies have been carried out in tropical areas, whereas less information is available from western countries with low intensity of helminth infections. in the netherlands, the infection of pigs with ascaris suum is very common, particularly on pig farms with outdoor facilities. this helminth can also infect humans, causing visceral larva migrans. this study aims at determining the pr ... | 2009 | 19644714 |
| ige cross-reactivity between ascaris and domestic mite allergens: the role of tropomyosin and the nematode polyprotein aba-1. | analysis of cross-reactivity between the nematode ascaris ssp. and dust mites, two important allergen sources in the tropics, will contribute in understanding their influence on asthma and atopy. the objective of this study was to investigate immunoglobulin e (ige) cross-reactivity between ascaris and two domestic mites in the tropics. | 2009 | 19624559 |
| the nicotinic acetylcholine receptors of the parasitic nematode ascaris suum: formation of two distinct drug targets by varying the relative expression levels of two subunits. | parasitic nematodes are of medical and veterinary importance, adversely affecting human health and animal welfare. ascaris suum is a gastrointestinal parasite of pigs; in addition to its veterinary significance it is a good model of the human parasite ascaris lumbricoides, estimated to infect approximately 1.4 billion people globally. anthelmintic drugs are essential to control nematode parasites, and nicotinic acetylcholine receptors (nachrs) on nerve and muscle are the targets of cholinergic a ... | 2009 | 19609360 |
| evolution of asabf (ascaris suum antibacterial factor)-type antimicrobial peptides in nematodes: putative rearrangement of disulfide bonding patterns. | asabf (ascaris suum antibacterial factor)-type antimicrobial peptides are defensin-like cysteine-rich peptides that are widely distributed in the phylum nematoda. in known members of asabf-type antimicrobial peptides, an array consisting of eight cysteine residues is completely conserved. in this study, we report a novel member asabf-6cys-alpha, which contains only six cysteine residues, in the pig round worm a. suum. the two cysteine residues deleted in asabf-6cys-alpha were not identical to a ... | 2009 | 19560487 |
| ascaris suum infection negatively affects the response to a mycoplasma hyopneumoniae vaccination and subsequent challenge infection in pigs. | since their first introduction more than a century ago, vaccines have become one of the most cost-effective tools to prevent and manage infectious diseases in human and animal populations. it is vital to understand the possible mechanisms that may impair optimal vaccine efficacy. the hypothesis posed in this study was that a concurrent ascaris suum infection of pigs vaccinated with a mycoplasma hyopneumoniae (mh) vaccine would modulate the protective immune response to a subsequent challenge inf ... | 2009 | 19524617 |
| effects of nickel on eosinophil survival. | accessories, watches, coins and other items containing metal sometimes cause contact dermatitis and metal allergy. among metals, nickel in alloys is ionized by sweat on the surface of the skin and exhibits particularly marked irritancy and allergenicity. although eosinophils play important roles in allergy, the effects of nickel on eosinophils have not been elucidated. | 2009 | 19494506 |
| a prostaglandin d2 receptor antagonist modifies experimental asthma in sheep. | prostaglandin (pg) d(2) is the major cylooxygenase metabolite released by mast cells upon allergen stimulation, and elicits responses through either the prostanoid dp1 receptor and/or the chemoattractant receptor homologous molecule expressed on t-helper type 2 (th2) cells (crth2/dp2). experimental evidence suggests that stimulation of one or both these receptors contributes to asthma pathophysiology. | 2009 | 19486034 |
| sequence analysis of bs-ag2 gene from baylisascaris schroederi of giant panda and evaluation of the efficacy of a recombinant bs-ag2 antigen in mice. | the gene of bs-ag2 from baylisascaris schroederi was amplified, cloned and sequenced. sequence analysis indicated that the nucleotide sequences of the bs-ag2 from adult, l2 and l3 of b. schroederi were completely identical. a homology search performed by blast revealed that bs-ag2 shared the highest amino acid sequence identity with as16 protein from ascaris suum (94%). the recombinant bs-ag2 proteins can be successfully expressed in escherichia coli bl21 (de3). the rbs-ag2 was used to evaluate ... | 2009 | 19428912 |
| cytogenetic study of ascaris trypsin inhibitor in cultured human lymphocytes with metabolic activation. | the trypsin inhibitor (ati) isolated from gastrointestinal nematode ascaris suum was tested in vitro for induction of chromosome aberrations and sister chromatid exchanges (sce). genotoxicity assessment of purified ati was carried out on metaphase plates received from peripheral blood lymphocyte macroculture (48 h test of structural chromosome aberrations and 72 h test of sce) with exogenous metabolic activation. ati was tested in dose of 25, 50 and 100 microg per ml of culture. kinetics of cell ... | 2009 | 19417546 |
| allergen dose dependency of the early- and late-phase cutaneous response in the cynomolgus monkey. | cutaneous administration of allergen provides a means to confirm an allergic status, investigate the pathogenesis of allergic diseases, and/or provide a mechanism to evaluate the benefit of new potential therapeutics. | 2009 | 19400909 |
| the geometry and motion of nematode sperm cells. | the nematode sperm cell crawls by recycling major sperm protein (msp) from dimers into subfilaments, filaments, and filament complexes, as a result of thermal writhing in the presence of hydrophobic patches. polymerization near leading edges of the cell intercolates msp dimers onto the tips of growing filament complexes, forcing them against the cell boundary, and extending the cytoskeleton in the direction of motion. strong adhesive forces attach the cell to the substrate in the forward part of ... | 2009 | 19396868 |
| bioinformatic analysis of abundant, gender-enriched transcripts of adult ascaris suum (nematoda) using a semi-automated workflow platform. | expressed sequence tag (est) data representing transcripts with a high level of differential hybridization in suppressive-subtractive hybridization (ssh)-based microarray analysis between adult female and male ascaris suum were subjected to detailed bioinformatic analysis. a total of 361 ests clustered into 209 sequences, of which 52 and 157 represented transcripts that were enriched in female and male a. suum, respectively. thirty (57.7%) of the 'female' subset of 52 sequences had orthologues/h ... | 2009 | 19361552 |
| localized th1-, th2-, t regulatory cell-, and inflammation-associated hepatic and pulmonary immune responses in ascaris suum-infected swine are increased by retinoic acid. | pigs infected with ascaris suum or controls were given 100 microg (low-dose) or 1,000 microg (high-dose) all-trans retinoic acid (atra)/kg body weight in corn oil or corn oil alone per os on days after inoculation (dai) -1, +1, and +3 with infective eggs. treatment with atra increased interleukin 4 (il4) and il12p70 in plasma of infected pigs at 7 dai and augmented bronchoalveolar lavage (bal) eosinophilia observed at 7 and 14 dai. to explore potential molecular mechanisms underlying these obser ... | 2009 | 19332534 |
| ascaris suum nadh-methemo(myo)globin reductase systems recovering differential functions of hemoglobin and myoglobin, adapting to environmental hypoxia. | we reported previously that ascaris suum cytochrome b5, specifically expressed in this nematode at the adult stage and dually localized in extracellular perienteric fluid and hypodermis, is involved in both perienteric nadh-methemoglobin and cytosolic nadh-metmyoglobin reduction, where cytochrome b5 functions as an electron carrier between nadh-mediated cytochrome b5 reductase and substrates, methemo(myo)globins to reduce the nonfunctional globins back to functional ferrous hemo(myo)globins. to ... | 2009 | 19332145 |
| identification of an ascaris g protein-coupled acetylcholine receptor with atypical muscarinic pharmacology. | acetylcholine (ach) is a neurotransmitter/neuromodulator in the nematode nervous system and induces its effects through interaction with both ligand-gated ion channels (lgics) and g protein-coupled receptors (gpcrs). the structure, pharmacology and physiological importance of lgics have been appreciably elucidated in model nematodes, including parasitic species where they are targets for anthelmintic drugs. significantly less, however, is understood about nematode ach gpcrs, termed gars (g prote ... | 2009 | 19327362 |
| mitochondrial dna sequence divergence among meloidogyne incognita, romanomermis culicivorax, ascaris suum, and caenorhabditis elegans. | mitochondrial dna sequences were obtained from the nadh dehydrogenase subunit 3 (nd3), large rrna, and cytochrome b genes from meloidogyne incognita and romanomermis culicivorax. both species show considerable genetic distance within these same genes when compared with caenorhabditis elegans or ascaris suum, two species previously analyzed. caenorhabditis, ascaris, and meloidogyne were selected as representatives of three subclasses in the nematode class secernentea: rhabditia, spiruria, and dip ... | 1993 | 19279810 |
| major sperm protein genes from globodera rostochiensis. | three genes in the major sperm protein (msp) gene family from the potato cyst nematode globodera rostochiensis were cloned and sequenced. in contrast to the absence of introns in caenorhabditis elegans msp genes, these genes in g. rostochiensis contained a 57 nucleotide intron, with normal exon-intron boundaries, in the same relative location as the intron in onchocerca volvulus. the msp genes of g. rostochiensis had putative caat, tata, and polyadenylation signals. the predicted g. rostochiensi ... | 1993 | 19279808 |
| a simple 1-d physical model for the crawling nematode sperm cell. | we develop a one-dimensional physical model of the crawling movement of simple cells: the sperm of a nematode, ascaris suum. the model is based on the assumptions that polymerization and bundling of the cytoskeletal filaments generate the force for extension at the front, and that energy stored in the gel formed from the filament bundles is subsequently used to produce the contraction that pulls the rear of the cell forward. the model combines the mechanics of protrusion and contraction with che ... | 2003 | 19277214 |
| microevolutionary patterns and molecular markers: the genetics of geographic variation in ascaris suum. | molecular markers have been used only rarely to characterize the population genetic structure of nematodes. published studies have suggested that different taxa may show distinct genetic architectures. isoenzyme and rapd markers have been used to investigate geographic variation of ascaris suum at the level of infrapopulations (nematodes within individual hosts), within localities, and among geographic regions. independent estimates of genetic differentiation among population samples based on is ... | 1996 | 19277145 |
| maintenance of dispersed reproductive cells from male and female ascaris suum. | in vitro cultivation of tissues and cells provides an experimental methodology to define and manipulate physiological mechanisms that are not possible with in vivo techniques. tissues from the germinative-growth zones of adult ascaris suum gonads were excised and minced, and then enzymatically dispersed and transferred to an artificial, perienteric fluid-fetal calf-serum-medium complex. cells were maintained in a viable state for 8 days, with medium replacement every 48 hours. during this period ... | 1997 | 19274146 |
| prophylactic effects of omega-3 polyunsaturated fatty acids and luteolin on airway hyperresponsiveness and inflammation in cats with experimentally-induced asthma. | the aim of this study was to assess the preventive effects of omega-3 polyunsaturated fatty acids (omega3 pufa) and luteolin supplementation on allergen-induced inflammation in eight ascaris suum (as)-sensitised cats. airway responsiveness (ar) tests were performed and venous blood and bronchoalveolar lavage fluid (balf) collected before and following a single (as-stimulated) allergen exposure, as well as at the end of a 4-week treatment period, which was followed by a second as-challenge. the o ... | 2010 | 19231257 |
| [pulmonary eosinophilia caused by visceral larva due to ascaris suum]. | 2009 | 19227919 | |
| high heritability for ascaris and trichuris infection levels in pigs. | aggregated distributions of macroparasites within their host populations are characteristic of most natural and experimental infections. we designed this study to measure the amount of variation that is attributable to host genetic factors in a pig-helminth system. in total, 195 piglets were produced after artificial insemination of 19 sows (danish landrace-yorkshire crossbreds) with semen selected from 13 individual duroc boars (1 or 2 sows per boar; mean litter size: 10.3; 5-14 piglets per lit ... | 2009 | 19142203 |
| can helminths or helminth-derived products be used in humans to prevent or treat allergic diseases? | recent epidemiological and experimental data indicate that infection with helminths can protect humans from the development of allergic disorders by immunosuppressive mechanisms that involve the induction of il-10 and/or regulatory t cells. furthermore, helminth-derived immune modulators suppress allergic responses in mice. trichuris suis therapy has been shown to be safe and efficacious in treating inflammatory bowel disease in humans. has the time come to treat patients who have allergic disea ... | 2009 | 19138565 |
| the level of sugars and synthesis of trehalose in ascaris suum tissues. | the activities of trehalose-6-phosphate synthase (tps) and trehalose-6-phosphate phosphatase (tpp) were observed in muscles, individual parts of the reproductive system and haemolymph of ascaris suum. the highest activity of tps was detected in the upper uterus, while the lowest activity of tps was detected in the ovary and oviduct of the nematode. relatively high activity was detected in muscles, haemolymph and two remaining parts of the uterus. the tpp activity was the highest in lower length ... | 2009 | 19138450 |
| the dynamics of genetically marked ascaris suum infections in pigs. | summary: the genotypes of both host and parasite may influence the outcome of parasitic infections, but few attempts have been made to quantify the effect of parasite genotype on macroparasite infections of socio-economic importance. we examined variation in particular traits during the infection in pigs with the parasitic nematode ascaris suum. we infected 26 pigs with mixtures of equal proportions of embryonated eggs from 4 single female worms each with a unique mtdna haplotype--the eggs from ... | 2009 | 19091154 |
| the house fly (musca domestica) as a potential vector of metazoan parasites caught in a pig-pen in germany. | in the present study a total of 224 specimens of the synanthropic house fly (musca domestica) were caught in a pig-pen of an organic farmer in dormagen (germany). the flies were examined for their potential as a carrier of metazoan parasites. on the exoskeletons and in the intestines of the flies the eggs and/or larvae of four endoparasite nematode species of domestic pigs (ascaris suum, strongyloides ransomi, metastrongylus sp., undetermined strongylida) were isolated. also one ectoparasite spe ... | 2009 | 19081196 |
| inactivation of ascaris eggs in source-separated urine and feces by ammonia at ambient temperatures. | sustainable management of toilet waste must prevent disease transmission but allow reuse of plant nutrients. inactivation of uterus-derived ascaris suum eggs was studied in relation to ammonia in source-separated urine without additives and in human feces to which urea had been added, in order to evaluate ammonia-based sanitation for production of safe fertilizers from human excreta. urine was used concentrated or diluted 1:1 and 1:3 with tap water at 4, 14, 24, and 34 degrees c. fecal material, ... | 2009 | 19060175 |
| preclinical safety and pharmacology of an anti-human interleukin-13 monoclonal antibody in normal macaques and in macaques with allergic asthma. | interleukin-13 (il-13) plays a central role in chronic airway diseases, including asthma. these studies were conducted to evaluate the safety of administration of a human anti-il-13 monoclonal antibody (mab) to normal macaques and in macaques with allergic asthma. in addition, serum and bronchioalveolar lavage fluid were collected from allergic cynomolgus macaques in order to identify potential surrogate markers of il-13 pharmacology that could be useful for subsequent clinical trials. in vitro ... | 2008 | 19037805 |
| dose-dependent impact of larval ascaris suum on host body weight in the mouse model. | ascaris lumbricoides and ascaris suum are important helminth parasites of humans and pigs, respectively. although it is now well established that the presence of mature adult worms in the host intestine contributes to significant nutritional morbidity, the impact of larval migratory ascariasis is far less well understood. the development of a mouse model to explore susceptibility and resistance to larval ascariasis in the lungs provided an opportunity to observe the impact of larval migration on ... | 2009 | 19021921 |
| pas-1, a protein from ascaris suum, modulates allergic inflammation via il-10 and ifn-gamma, but not il-12. | helminths and their products have a profound immunomodulatory effect upon the inductive and effector phases of inflammatory responses, including allergy. we have demonstrated that pas-1, a protein isolated from ascaris suum worms, has an inhibitory effect on lung allergic inflammation due to its ability to down-regulate eosinophilic inflammation, th2 cytokine release and ige antibody production. here, we investigated the role of il-12, ifn-gamma and il-10 in the pas-1-induced inhibitory mechanis ... | 2008 | 19008120 |
| hepatic lesions caused by migrating larvae of ascaris suum in chickens. | group a consisted of chickens infected with a single dose of ascaris suum and group b of chickens infected with two successive doses. at days 1, 3, 7, 14 and 21 after the first or second infection dose, six chickens from each group were sacrificed. in both groups, larvae were recovered from the livers on days 1, 3, and 7 and lungs on days 3 and 7. no larvae were detected in chickens on day 14. clear white lesions were noticed only on the livers from chickens of group b at day 7 but had disappear ... | 2008 | 18981674 |
| fate of pathogenic microorganisms and indicators in secondary activated sludge wastewater treatment plants. | this study was undertaken to investigate the removal of pathogenic microorganisms and their indicators in a laboratory scale biological treatment system that simulated the secondary treatment process of a wastewater treatment plant (wwtp). four groups of microorganisms including bacteria, viruses, protozoa and helminths as well as the selected indicators were employed in the investigation. the results demonstrated that approximately 2-3 log10 removal of the microbial indicators was achieved in t ... | 2009 | 18977580 |
| some aspects of nematode infection in pigs from small herds. | two traditionally maintained, small herds from southern poland, with 8 and 12 sows, respectively, were surveyed coprologically during 2006-2007. in one of the herds, while deworming a group of sows with levamisole, faecal samples were collected on day -7, day 0 (the day of treatment) and day 10, in order to assess the therapeutic effect of the drug. coprological investigation was performed also in 26 fatteners originating from other small farms and slaughtered in a local abattoir, with their int ... | 2008 | 18942544 |
| molecular characterization and real-time pcr transcriptional analysis of dictyocaulus viviparus major sperm proteins. | major sperm proteins (msps) represent a protein family occurring in nematodes only. identification of the 3' and 5' untranslated region (utr) completed the so far partial msp complementary dna sequences of the bovine lungworm dictyocaulus viviparus. the full-length transcript contains sequence tracts consistent with the kozak and polyadenylation consensus sequence. on genomic level, three full-length sequences differing in three nucleotides were determined containing a 65-bp phase zero intron. c ... | 2009 | 18853187 |
| oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (as16) of ascaris suum fused with cholera toxin b subunit. | cereal crops such as maize and rice are considered attractive for vaccine production and oral delivery. here, we evaluated the rice oryza sativa for production of as16-an antigen protective against the roundworm ascaris suum. the antigen was produced as a chimeric protein fused with cholera toxin b subunit (ctb), and its expression level in the endosperm reached 50 microg/g seed. feeding the transgenic (tg) rice seeds to mice elicited an as16-specific serum antibody response when administered in ... | 2009 | 18763047 |
| effects of sdpnflrf-amide (pf1) on voltage-activated currents in ascaris suum muscle. | helminth infections are of significant concern in veterinary and human medicine. the drugs available for chemotherapy are limited in number and the extensive use of these drugs has led to the development of resistance in parasites of animals and humans (geerts and gryseels, 2000; kaplan, 2004; osei-atweneboana et al., 2007). the cyclooctadepsipeptide, emodepside, belongs to a new class of anthelmintic that has been released for animal use in recent years. emodepside has been proposed to mimic th ... | 2009 | 18760280 |
| role of residues in the adenosine binding site of nad of the ascaris suum malic enzyme. | ascaris suum mitochondrial malic enzyme catalyzes the divalent metal ion dependent conversion of l-malate to pyruvate and co(2), with concomitant reduction of nad(p) to nad(p)h. in this study, some of the residues that form the adenosine binding site of nad were mutated to determine their role in binding of the cofactor and/or catalysis. d361, which is completely conserved among species, is located in the dinucleotide-binding rossmann fold and makes a salt bridge with r370, which is also highly ... | 2008 | 18725329 |
| helminth products as a potential therapeutic strategy for inflammatory diseases. | helminths secrete several molecules that can modulate the immune responses, favoring their evasion and perpetuate their survival in the host. these molecules interfere with antigen presentation, cell proliferation and activation, antibody production, cause cell death, and stimulate regulatory responses. here, we focus on some helminth products and address their immunomodulatory effects in the host immune system and, also, we describe some anti-inflammatory properties of an ascaris suum-derived i ... | 2008 | 18691141 |
| the mitochondrial genome of toxocara canis. | toxocara canis (ascaridida: nematoda), which parasitizes (at the adult stage) the small intestine of canids, can be transmitted to a range of other mammals, including humans, and can cause the disease toxocariasis. despite its significance as a pathogen, the genetics, epidemiology and biology of this parasite remain poorly understood. in addition, the zoonotic potential of related species of toxocara, such as t. cati and t. malaysiensis, is not well known. mitochondrial dna is known to provide g ... | 2008 | 18682828 |
| intestinal transcriptomes of nematodes: comparison of the parasites ascaris suum and haemonchus contortus with the free-living caenorhabditis elegans. | the nematode intestine is a major organ responsible for nutrient digestion and absorption; it is also involved in many other processes, such as reproduction, innate immunity, stress responses, and aging. the importance of the intestine as a target for the control of parasitic nematodes has been demonstrated. however, the lack of detailed knowledge on the molecular and cellular functions of the intestine and the level of its conservation across nematodes has impeded breakthroughs in this applicat ... | 2008 | 18682827 |
| parasitic nematode modulation of allergic disease. | incidence of allergic diseases such as asthma has increased at an alarming rate in western countries in the past few decades. however, in parts of the world in which parasitic nematode infections are highly prevalent, allergy remains uncommon. hence, it has been postulated that nematodes offer humans protection against this type of disease. this article reviews the evidence to support this idea, considering data from human studies and results from investigations into the protective effects of ne ... | 2008 | 18682103 |
| the actions of caenorhabditis elegans neuropeptide-like peptides (nlps) on body wall muscle of ascaris suum and pharyngeal muscle of c. elegans. | the actions of six neuropeptide-like peptides (nlps) were investigated on ascaris suum dorsal body wall muscle and caenorhabditis elegans pharyngeal muscle. the nlps tested on a. suum muscle, viz, nlp-1a, nlp-2a and b, had no direct effect on the muscle but all reversibly reduced acetylcholine (ach)-induced contractions of the muscle, nlp-2a being the most potent, with an ic50 value of 78 microm. nlp-2a also reversibly reduced ach-induced depolarizations of a. suum muscle resting membrane potent ... | 2008 | 18652392 |
| major sperm protein as a diagnostic antigen for onchocerciasis. | onchocerciasis, also known as river blindness, is the second leading infectious cause of blindness worldwide. in order to successfully control this disease, the development of efficient diagnostic tools as well as effective treatments is imperative. a number of proteins have been proposed as vaccine and diagnostic candidates, yet none have been successfully advanced to the point of general clinical use. we have prepared major sperm protein 2 (msp2) from onchocerca volvulus as a possible diagnost ... | 2008 | 18632276 |
| neuropeptide-like protein diversity in phylum nematoda. | this study reports the identification of nematode neuropeptide-like protein (nlp) sequelogs from the genbank expressed sequence tag (est) database, using blast (basic local alignment search tool) search methodology. search strings derived from peptides encoded by the 45 known caenorhabditis elegans nlp genes were used to identify more than 1000 ests encoding a total of 26 multi-species nlp sequelogs. the remaining 18 nlps (nlp-4, -16, -24 through -36, -39, -41 and -45) were identified only in c. ... | 2008 | 18602104 |
| differential effects of dexamethasone on the proximal and distal lung response to antigen challenge in allergic cynomolgus monkeys. | the proximal and distal portions of the lungs may respond differently to antigen challenge and bronchodilator treatment. this difference may contribute to differences in actual and perceived efficacy of therapies. in this study we used the forced oscillation technique (fot) to measure impedance in the pulmonary system and discern the effects of antigen challenge on proximal (large airway) and distal (small airway and lung parenchyma) portions of the lung. in addition we treated the animals with ... | 2008 | 18569230 |
| genomic-bioinformatic analysis of transcripts enriched in the third-stage larva of the parasitic nematode ascaris suum. | differential transcription in ascaris suum was investigated using a genomic-bioinformatic approach. a cdna archive enriched for molecules in the infective third-stage larva (l3) of a. suum was constructed by suppressive-subtractive hybridization (ssh), and a subset of cdnas from 3075 clones subjected to microarray analysis using cdna probes derived from rna from different developmental stages of a. suum. the cdnas (n = 498) shown by microarray analysis to be enriched in the l3 were sequenced and ... | 2008 | 18560474 |
| [prediction of the time of development and survival of ascarid eggs in the soil in the kursk region]. | the paper shows that the currently available methods used to predict the time of development and survival of ascarid eggs in the soil, by analyzing its temperature and moisture (bioclimatograms and nomograms), provide no precise information. at the same time soil chemical and man-made pollution by heavy metals and the presence of geomagnetic fields are disregarded. improved bioclimatographic and nomographic methods are proposed, with the correction factors being kept in mind. | 2008 | 18557360 |
| a laboratory study of survival of selected microorganisms after heat treatment of biowaste used in biogas plants. | the aim of the study was to assess the effect of pasteurisation, as set by the european regulation ec 1774/2002, on selected pathogens and indicator organisms. unpasteurised substrate (biowaste), including animal by-products from a full-scale biogas plant was heat treated under laboratory conditions at 70 degrees c and 55 degrees c for 30 min and 60 min. heat treatment at 55 degrees c for 60 min was not sufficient to achieve a hygienically acceptable product. heat treatment at 70 degrees c for 3 ... | 2008 | 18513960 |
| the prevalence of gastrointestinal helminth infections in pigs in kenya. | the prevalence of helminth infection, species spectrum and worm burdens in kenyan pigs was examined. a total of 115 gastrointestinal tracts (git) from 61 growers and 54 adult pigs were examined between february 2005 and january 2006. seventy eight (67.8%) had one or more helminth parasites, of which thirty six (31.3%) were mixed infection. ten types of helminth parasites encountered in descending order of prevalence were, oesophagostomum dentatum (39.1%), trichuris suis (32.2%), ascaris suum (28 ... | 2008 | 18509940 |
| what are the infectious larvae in ascaris suum and trichuris muris? | in the present study, larvae of ascaris suum and trichuris muris were investigated by light and electron microscopy after incubation in a hatching medium containing 89% phosphate-buffered saline (ph 7.4), 10% rpmi-1640 and 1% sodiumhypochlorite at 40 and 37 degrees c, respectively. the larvae were obtained from fertilised eggs of the worms during defined phases of development (a. suum, 36th-50th day of development; t. muris, once a week from week 16 to 20). light and electron micrographs of the ... | 2008 | 18504615 |
| the complete mitochondrial genomes for three toxocara species of human and animal health significance. | studying mitochondrial (mt) genomics has important implications for various fundamental areas, including mt biochemistry, physiology and molecular biology. in addition, mt genome sequences have provided useful markers for investigating population genetic structures, systematics and phylogenetics of organisms. toxocara canis, toxocara cati and toxocara malaysiensis cause significant health problems in animals and humans. although they are of importance in human and animal health, no information o ... | 2008 | 18482460 |
| protective effect of an extract from ascaris suum in experimental arthritis models. | we investigated the effect of an extract from a helminth (ascaris suum) in zymosan-induced arthritis (zya) or collagen-induced arthritis (cia). rats and mice, respectively, received 1 mg and 0.1 mg zymosan intra-articularly (i.a.). test groups received an a. suum extract either per os (p.o.) or intraperitoneally (i.p.) 30 min prior to i.a. zymosan. controls received saline. hypernociception was measured using the articular incapacitation test. cell influx, nitrite, and cytokine levels were asses ... | 2008 | 18411290 |
| a novel small heat shock protein 12.6 (hsp12.6) from brugia malayi functions as a human il-10 receptor binding protein. | phage display cdna expression library of the third stage larvae (l3) of brugia malayi was screened for identifying target(s) that bound to the human interleukin-10 receptor (huil10r). this iterative screening identified an insert that showed significant homology to caenorhabditis elegans hsp12.6. the gene was designated b. malayi hsp12.6 (bmhsp12.6) and has orthologues in several gastrointestinal nematode genome (ancylostoma caninum, ascaris lumbricoides and ascaris suum) but the gene or gene pr ... | 2008 | 18395809 |
| glucocorticoids ameliorate antigen-induced bronchial smooth muscle hyperresponsiveness by inhibiting upregulation of rhoa in rats. | to determine the mechanism(s) of the inhibitory effect of glucocorticoids on airway hyperresponsiveness in allergic bronchial asthma, the effects of systemic treatment with glucocorticoids on bronchial smooth muscle hyperresponsiveness and rhoa upregulation were investigated in rats with allergic bronchial asthma. rats were sensitized and repeatedly challenged with 2,4-dinitrophenylated ascaris suum antigen. animals were also treated with prednisolone or beclomethasone (each 10 mg/kg, i.p.) once ... | 2008 | 18391481 |
| a novel technique for identification of ascaris suum cohorts in pigs. | the objective of the present study was to develop a fast, cheap and reliable technique for identifying different cohorts of the swine parasite, ascaris suum. a polymerase chain reaction linked restriction fragment length polymorphism (pcr-rflp) technique on mt-dna was used to identify unique haplotypes of four gravid a. suum females on agarose gels after eggs were recovered from each of the worms. each of four pigs was inoculated with 2000 embryonated eggs originating from one of the four identi ... | 2008 | 18387743 |
| the role of filament-packing dynamics in powering amoeboid cell motility. | although several models have been proposed to account for how cytoskeleton polymerization drives protrusion in cell motility, the precise mechanism remains controversial. here, we show that, in addition to force exerted directly against the membrane by growing filaments, the way elongating filaments pack also contributes to protrusion by generating an expansion of the cytoskeleton gel. tomography shows that filament packing in the major sperm protein (msp) -based nematode sperm-motility machiner ... | 2008 | 18385381 |
| cloning and expression of ani s 9, a new anisakis simplex allergen. | the larvae of the nematode anisakis simplex parasitize seafood. when people eat raw or undercooked parasitized fish, they can suffer anisakiasis, an important immune human response to parasitic infection of the gastrointestinal tract. even more, allergic manifestations like angioedema, urticaria or anaphylaxis can occur in sensitized patients. the aim of this work was to clone ani s 9-cdna and overproduce this recombinant allergen in escherichia coli. the finding of this allergen was an unexpect ... | 2008 | 18378015 |
| modeling of asthma, copd and cystic fibrosis in sheep. | sheep naturally allergic to ascaris suum antigen have been used to study the pathophysiology of asthma and more recently allergic rhinitis, chronic obstructive pulmonary disease (copd) and cystic fibrosis. the utility of the model as it relates to the study of these diseases is discussed. | 2008 | 18339565 |
| interleukin-13 neutralization by two distinct receptor blocking mechanisms reduces immunoglobulin e responses and lung inflammation in cynomolgus monkeys. | interleukin (il)-13 is a key cytokine driving allergic and asthmatic responses and contributes to airway inflammation in cynomolgus monkeys after segmental challenge with ascaris suum antigen. il-13 bioactivity is mediated by a heterodimeric receptor (il-13ralpha1/il-4ralpha) and can be inhibited in vitro by targeting il-13 interaction with either chain. however, in cytokine systems, in vitro neutralization activity may not always predict inhibitory function in vivo. to address the efficacy of t ... | 2008 | 18337474 |
| visceral helminths of wild boars (sus scrofa leucomystax) in japan, with special reference to a new species of the genus morgascaridia inglis, 1958 (nematoda: schneidernematidae). | twenty-nine japanese wild boars (sus scrofa leucomystax), collected during the hunting seasons of 2005 and 2006 in the western part of the mainland of japan (honshu), were examined for their visceral helminths. eighteen helminth parasites were prevalent in them, including 17 nematoda species (metastrongylus elongatus, metastrongylus salmi, metastrongylus asymmetricus, metastrongylus pudendotechus, stephanurus dentatus, gnathostoma doloresi, physocephalus sexulata, ascarops strongylina, capillari ... | 2008 | 18328113 |
| lovastatin inhibits bronchial hyperresponsiveness by reducing rhoa signaling in rat allergic asthma. | recent studies revealed an importance of a monomeric gtp-binding protein, rhoa, in contraction of bronchial smooth muscle (bsm). rhoa and its downstream have been proposed as a new target for the treatment of airway hyperresponsiveness in asthma. statins are known to inhibit the functional activation of rhoa via the depletion of geranylgeranylpyrophosphate. to determine the beneficial effects of statins on the airway hyperresponsiveness in allergic bronchial asthma, we investigated the effects o ... | 2008 | 18296496 |
| proper positioning of the nicotinamide ring is crucial for the ascaris suum malic enzyme reaction. | the mitochondrial nad-malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate and co2. the role of the dinucleotide substrate in oxidative decarboxylation is probed in this study using site-directed mutagenesis to change key residues that line the dinucleotide binding site. mutant enzymes were characterized using initial rate kinetics, and isotope effects were used to obtain information on the contribution of these residues to binding energy and catalysis. results obtained for ... | 2008 | 18215074 |
| effects of the muscarinic agonist, 5-methylfurmethiodide, on contraction and electrophysiology of ascaris suum muscle. | contraction and electrophysiological effects of 5-methylfurmethiodide (mfi), a selective muscarinic agonist in mammals, were tested on ascaris suum muscle strips. in a contraction assay, mfi produced weak contraction and was less potent than levamisole and acetylcholine. atropine (3microm) a non-selective muscarinic antagonist in mammalian preparations, did not affect contractions produced by mfi. mecamylamine (3microm) a nicotinic antagonist in a. suum preparations, blocked the mfi contractions ... | 2008 | 18206155 |
| prenatal toxicity of ascaris pepsin inhibitor in mice. | the developmental toxicity of pepsin inhibitor isolated from ascaris suum, a gastrointestinal nematode parasite, was evaluated. an embryo-fetal development study was conducted in balb/c mice. groups of 21 mated females were treated by intraperitoneal injection (0.3 ml/30 g body weight) with 0.9% nacl solution vehicle or isolated ascaris pepsin inhibitor (api) at dose levels of 50, 100, 150 or 200mg/kg body weight/day on gestation days (gd) 6-15. maternal food consumption, body weight, and clinic ... | 2008 | 18191939 |
| shortening of the induction period of allergic asthma in cynomolgus monkeys by ascaris suum and house dust mite. | the development of non-human primate models of asthma requires a period of time (e.g., 0.5-1 year). to develop the models in a short period, male cynomolgus monkeys were sensitized with dinitrophenyl-ascaris suum (dnp-as) allergen by intraperitoneal and intramuscular injection and by intratracheal inhalation. all sensitized animals developed positive intradermal skin reaction to dnp-as. sensitization elevated allergen-specific ige levels in serum, the number of ccr4-positive t helper lymphocytes ... | 2008 | 18187925 |
| in vitro evaluation of the effect of the nematophagous fungi duddingtonia flagrans, monacrosporium sinense, and pochonia chlamydosporia on ascaris suum eggs. | the in vitro effect of four isolates of the nematophagous fungi duddingtonia flagrans (ac 001), monacrosporium sinense (sf 53), and pochonia chlamydosporia (vc 1 and vc 4) on eggs of ascaris suum was evaluated. one hundred thousand a. suum eggs were plated on 2% water-agar with the grown isolates and control without fungus. after 7, 14, and 21 days, 100 eggs were removed and classified according to the following parameters: type 1, lytic effect without morphological damage to eggshell; type 2, l ... | 2008 | 18185942 |
| ascaris suum cytochrome b5, an adult-specific secretory protein reducing oxygen-avid ferric hemoglobin. | the anaerobic parasitic nematode ascaris suum has an oxygen-avid hemoglobin in the perienteric fluid, the biological function of which remains elusive. here, we report that ascaris cytochrome b5 is expressed specifically in the intestinal parasitic stage and is secreted into the perienteric fluid, thus co-localizing with ascaris hemoglobin. we also found that cytochrome b5 reduces ascaris non-functioning ferric methemoglobin more efficiently than mammalian methemoglobin. furthermore, a computer ... | 2008 | 18158911 |
| experimental hatching of the eggs of ascaris suum. | 1948 | 18102225 | |
| chemokine receptor 2 blockade prevents asthma in a cynomolgus monkey model. | the pathophysiology of asthma is characterized by accumulation and activation of several cell types in the lung, which correlates with coordinated production of specific cytokines and chemokines. to study the effect of selective ccr2 chemokine receptor blockade on leukocyte recruitment to the lung and on bronchial function, we used a nonhuman primate model of allergic airway disease that closely resembles human asthma. allergic cynomolgus monkeys were treated with the antagonist anti-ccr2 (ccr2- ... | 2008 | 18029547 |
| multiple roles of arginine 181 in binding and catalysis in the nad-malic enzyme from ascaris suum. | the nad-malic enzyme catalyzes the oxidative decarboxylation of l-malate. structures of the enzyme indicate that arginine 181 (r181) is within hydrogen bonding distance of the 1-carboxylate of malate in the active site of the enzyme and interacts with the carboxamide side chain of the nicotinamide ring of nadh, but not with nad+. data suggested r181 might play a central role in binding and catalysis in malic enzyme, and it was thus changed to lysine and glutamine to probe its potential function. ... | 2007 | 18027982 |
| eosinophilic cholecystitis as a rare manifestation of visceral larva migrans. | eosinophilic cholecystitis is an infrequent form of cholecystitis. the etiology of eosinophilic cholecystitis is still obscure, and it is sometimes accompanied with several complications, but a simultaneous onset with pericarditis is very rare. we would like to make an alternative interpretation of our recent report "kaji k, yoshiji h, yoshikawa m, yamazaki m, ikenaka y, noguchi r, sawai m, ishikawa m, mashitani t, kitade m, kawaratani h, uemura m, yamao j, fujimoto m, mitoro a, toyohara m, yosh ... | 2007 | 18023114 |
| analysis of binding interactions of pepsin inhibitor-3 to mammalian and malarial aspartic proteases. | the nematode ascaris suum primarily infects pigs, but also causes disease in humans. as part of its survival mechanism in the intestinal tract of the host, the worm produces a number of protease inhibitors, including pepsin inhibitor-3 (pi3), a 17 kda protein. recombinant pi3 expressed in e. coli has previously been shown to be a competitive inhibitor of a subgroup of aspartic proteinases: pepsin, gastricsin and cathepsin e. the previously determined crystal structure of the complex of pi3 with ... | 2007 | 18004881 |
| benzenesulfonamide indole inhibitors of cytosolic phospholipase a2alpha: optimization of in vitro potency and rat pharmacokinetics for oral efficacy. | the synthesis and structure-activity relationship of a series of benzenesulfonamide indole inhibitors of cpla(2)alpha are described. substitution of the benzenesulfonamide led to analogues with 50-fold improvement in potency versus the unsubstituted benzenesulfonamide lead compound. rat pharmacokinetics in a minimal formulation was used to prioritize compounds, leading to the discovery of a potent inhibitor of cpla(2)alpha with oral efficacy in models of rat carrageenan paw edema and ascaris suu ... | 2008 | 17998165 |
| decontamination by anaerobic stabilisation of the environment contaminated with enteronematode eggs toxocara canis and ascaris suum. | investigations were carried out under operating conditions of field composting factory in brezno (slovak republic) to determine the effect of anaerobic stabilization of organic wastes from public areas on the survival of model helminth toxocara canis and ascaris suum eggs. due to anaerobic conditions, low temperature, low c:n ratio and changes in physical and chemical properties of organic waste, less than 64% of a. suum eggs remained viable after 150 days of stabilisation. the anaerobic stabili ... | 2008 | 17976979 |
| western blot antibody determination in sera from patients diagnosed with anisakis sensitization with different antigenic fractions of anisakis simplex purified by affinity chromatography. | using western blot techniques, the specificities of crude and purified (pak and pas) anisakis simplex antigens were compared against 24 sera from patients diagnosed with anisakis sensitization. all patients recognized a 60 kda protein against the a. simplex crude extract, while 37.5% and 12.5% reacted with proteins of 40 and 25 kda, respectively, when igg was tested. in the case of ige determination, 41.6% of sera were negative, while 12.5% and 20.8% appeared to cross-react against toxocara cani ... | 2007 | 17974043 |
| helminths of wild boar in the isolated population close to the northern border of its habitat area. | one hundred wild boars (sus scrofa) from a geographically isolated population on the island saaremaa of western estonia were examined for visceral helminths. seven helminth species, metastrongylus pudendotectus, m. salmi, m. elongatus, ascaris suum, trichuris suis, dicrocoelium dendriticum and taenia hydatigena larva, were found. the predominant helminths discovered were lung nematodes (prevalence 82%, mean intensity 96.2 per animal). a significant negative correlation was observed between the w ... | 2007 | 17964726 |
| a mouse fcgamma-fcepsilon protein that inhibits mast cells through activation of fcgammariib, sh2 domain-containing inositol phosphatase 1, and sh2 domain-containing protein tyrosine phosphatases. | a human fcgamma-fcepsilon fusion protein (ge2) designed to inhibit fcepsilonri signaling by coaggregating fcepsilonri with the inhibitory receptor fcgammariib has been shown to inhibit mast cell activation and block cutaneous anaphylaxis. a critical issue remained as to whether the mechanism of ge2 inhibition is competition for ige binding or inhibitory signaling through fcgammariib. | 2008 | 17949802 |
| decursin and decursinol angelate selectively inhibit nadh-fumarate reductase of ascaris suum. | nadh-fumarate reductase (nfrd) is a key enzyme in many anaerobic helminths. decursin and decursinol angelate have been isolated from the roots of angelica gigas nakai (apiaceae) as nfrd inhibitors. they inhibited ascaris suum nfrd with ic (50) values of 1.1 and 2.7 microm, respectively. their target is the electron transport enzyme complex i. since the inhibitory activities of decursin against bovine heart complexes are weak, it is a selective inhibitor of the nematode complex i. in contrast, de ... | 2007 | 17948169 |
| change of subunit composition of mitochondrial complex ii (succinate-ubiquinone reductase/quinol-fumarate reductase) in ascaris suum during the migration in the experimental host. | the mitochondrial metabolic pathway of the parasitic nematode ascaris suum changes dramatically during its life cycle, to adapt to changes in the environmental oxygen concentration. we previously showed that a. suum mitochondria express stage-specific isoforms of complex ii (succinate-ubiquinone reductase: sqr/quinol-fumarate reductase: qfr). the flavoprotein (fp) and small subunit of cytochrome b (cybs) in adult complex ii differ from those of infective third stage larval (l3) complex ii. howev ... | 2008 | 17933581 |