Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| microbiological conversion of a beta- and gamma-eudesmol mixture by rhizopus. | a mixture of beta- and gamma-eudesmols was microbiologically biotransformed by rhizopus stolonifer atcc 6227. positions-2 and 3, in both substrates, proved to be accessible by hydroxylase enzyme. four different metabolites were isolated and their structures were elucidated by different spectroscopic methods. the structures of these metabolites were established as eudesma-3-en-2 beta,11-diol; eudesma-4-en-3 beta,11-diol; eudesma-4(15)-en-2 beta,11-diol and eudesma-4(15)-en-3 beta,11-diol. | 2002 | 11836934 |
| control of post-harvest decay of apples by pre-harvest and post-harvest application of ammonium molybdate. | ammonium molybdate was tested as a potential fungicide for use in apples (cv golden delicious) against blue and grey mould, important post-harvest diseases of pome fruits. in tests in vivo at 20 degrees c, ammonium molybdate (15 mm) reduced lesion diameters of penicillium expansum, botrytis cinerea and rhizopus stolonifer by 84%, 88% and 100% respectively. when apples treated with ammonium molybdate were stored at 1 degree c for three months, a significant reduction in severity and incidence of ... | 2001 | 11802595 |
| biological control of postharvest pear diseases using a bacterium, pantoea agglomerans cpa-2. | epiphytic microorganisms isolated from the fruits and leaf surfaces of apples and pears were screened for antagonistic activity against penicillium expansum on pears. from 247 microorganisms tested for antagonistic properties against p. expansum, a bacterium strain identified as pantoea agglomerans (cpa-2) was selected. this bacterium was very effective against botrytis cinerea, p. expansum and rhizopus stolonifer. complete control at the three tested concentrations (2 x 10(7), 8 x 10(7) and 1 x ... | 2001 | 11759762 |
| structurally novel bioconversion products of the marine natural product sarcophine effectively inhibit jb6 cell transformation. | sarcophytol a (1) and b (2) (see chart 1) are cembrane-type diterpenes known to inhibit tumor promotion. indicative of this inhibitory response, we currently report sarcophytol a (1) mediates dose-dependent diminution of 12-o-tetradecanoylphorbol 13-acetate (tpa)-induced transformation of jb6 cells. moreover, a structurally related furanocembrane diterpene, sarcophine (3), isolated in good yield from the red sea soft coral sarcophyton glaucum, was also found to serve as an effective inhibitor of ... | 1998 | 11672397 |
| microbiological oxygenation of bridgehead azabicycloalkanes. | a series of n-substituted bridgehead azabicycloalkanes has been prepared and examined as substrates for microbiological oxygenation using the fungi beauveria bassiana, rhizopus nigricans, aspergillus ochraceus, and rhizopus arrhizus. oxygenation using b. bassiana of n-tosyl-7-azabicyclo[2.2.1]heptane gave n-[p-(hydroxymethyl)benzenesulfonyl]-7-azabicyclo[2.2.1]heptane (56% yield), of n-(phenyloxycarbonyl)-7-azabicyclo[2.2.1]heptane gave the 2-endo-ol (56% yield, 51% ee), of n-boc-7-azabicyclo[2. ... | 1997 | 11671535 |
| functional cloning, based on azole resistance in saccharomyces cerevisiae, and characterization of rhizopus nigricans redox carriers that are differentially involved in the p450-dependent response to progesterone stress. | the filamentous fungus rhizopus nigricans responds to treatment with progesterone by inducing p450-associated redox carriers. selection for azole resistance following expression of a cdna library constructed with rna from progesterone-treated r. nigricans in the yeast saccharomyces cerevisiae led to the identification of cpr1-fl and cyb5-1 cdnas, which code for functionally competent nadph-cytochrome p450 reductase and cytochrome b5, respectively. the central region (cpr2-cs) of an additional re ... | 2001 | 11523811 |
| microbial transformation of pyrethrosin. | microbial transformation of the germacranolide pyrethrosin (1) using rhizopus nigricans nrrl 1477 has resulted in the isolation of 6 alpha-acetoxy-1 beta,4 alpha-dihydroxy-5,7 alpha h,8 beta h-eudesm-11 beta,13-dihydro-8,12-olide (5), a new eudesmanolide-type metabolite, in addition to the previously reported eudesmanolides: 2, 3, 4, and 6. the structure elucidation of these metabolites was based primarily on 1d and 2d nmr analyses. the isolated metabolites exhibited cytotoxic, antifungal, and a ... | 2001 | 11520238 |
| biosorption of cr (vi) from aqueous solution by rhizopus nigricans. | the study was aimed to quantify the cr sorption ability of powdered biomass of rhizopus nigricans at the best operating conditions. the influence of solution ph, agitation, cr (vi) concentration, biomass dosage, contact time, biomass particle size and temperature were studied. the optimum ph for biosorption of cr (vi) was found to be 2.0. higher adsorption percentage was noted at lower initial concentrations of cr ions, while the adsorption capacity of the biomass increased with increasing conce ... | 2001 | 11396911 |
| ribonuclease rs from rhizopus stolonifer: lowering of optimum temperature in the presence of urea. | rnase rs showed an approx. 2-fold increase in its activity when incubated in the presence of 2 m urea at 37 degrees c. the increase in its activity, in the presence of urea, was comparable to the activity at its optimum temperature, i.e. 45 degrees c. compared to the native enzyme at 37 degrees c, the k(m) and v(max) of rnase rs at 45 degrees c and in the presence of 2 m urea at 37 degrees c showed an increase while k(cat)/k(m) decreased. arrhenius plots in the presence and absence of urea showe ... | 2001 | 11342027 |
| characterization of fungal 17beta-hydroxysteroid dehydrogenases. | to promote understanding of the evolution of the steroid hormone signalling and hydroxysteroid dehydrogenases (hsds), comparative characterization of fungal 17beta-hsds was performed. constitutive 17beta-hsd activity was determined in cytosols of the fungi: cochliobolus lunatus, pleospora herbarum, fusarium lini, trichoderma viride, mucor spinosus, rhizopus nigricans and pleurotus ostreatus. the reaction equilibrium in all species except p. ostreatus was shifted towards reduction. the preferenti ... | 2000 | 11126752 |
| regioselective enzymatic acylations of polyhydroxylated eudesmanes: semisynthesis, theoretical calculations, and biotransformation of cyclic sulfites. | different lipase enzymes have been tested in order to perform regioselective acetylations on the eudesmane tetrol from vulgarin. high yields (95%) of 1,12-diacetoxy derivative (4) were achieved in 1 h with candida antarctica lipase (cal). however, only the 12-acetyl derivative (6) was obtained in similar yield with mucor miehei (mml) or candida cylindracea (ccl) lipases. the enzymatic protection at c-1 and c-12 has been used to form eudesmane cyclic-sulfites between c-6 and c-4 atoms. the r/s-su ... | 2000 | 11101376 |
| optimization of the solid state fermentation process to obtain tempeh from hardened chickpeas (cicer arietinum l.). | solid state fermentation (ssf) represents a technological alternative for a great variety of cereals and legumes, or combination of them, to improve their nutritional quality and to obtain edible products with palatable sensorial characteristics. the objective of this work was to find the best conditions of fermentation temperature and time to obtain tempeh from hardened chickpeas (cicer arietinum l.) applying ssf. response surface methodology (rsm) was applied over three response variables (phy ... | 2000 | 11030476 |
| g-protein coupled progesterone receptors in the plasma membrane of fungus rhizopus nigricans. | we have demonstrated simultaneous existence of progesterone receptors and gtpase activity in the membranes prepared from the filamentous fungus rhizopus nigricans. the results obtained with pertussis toxin treated fungal mycelium suggest that these receptors do not couple to gi-go-proteins and play a role in the induction of steroid hydroxylating enzyme system by steroid substrates in the fungus. | 2000 | 11005662 |
| microcalorimetric investigation of the toxic action of cd(2+) on rhizopus nigricans growth. | the microcalorimetric bioassay for acute cellular toxicity is based on metabolic heat production from cultured cells. microcalorimetry is a quantitative, inexpensive, and versatile method for toxicology research. the biological response to toxicants is the inhibition of the heat production rate in cells and toxicity is expressed as the concentration of toxicant that is 50% effective in this inhibition (ic(50)). in this paper, the effect of cd(2+) on rhizopus nigricans growth was investigated at ... | 2000 | 10989139 |
| antifungal effects of hydrolysable tannins and related compounds on dermatophytes, mould fungi and yeasts. | a series of hydrolysable tannins and related compounds was evaluated for antifungal activities against filamentous fungi (epidermophyton floccosum; microsporum canis; microsporum gypseum; trichophyton mentagrophytes; trichophyton rubrum; trichophyton tonsurans; trichophyton terrestre; penicillium italicum; aspergillus fumigatus; mucor racemosus; rhizopus nigricans) and opportunistic yeasts (candida albicans; candida glabrata; candidata krusei; cryptococcus neoformans), using the agar dilution me ... | 2000 | 10928561 |
| thermochemical studies of the toxic actions of heavy metal ions on rhizopus nigricans. | by using a lkb2277 bioactivity monitor (heat conduction microcalorimeter), stopped-flow method, the thermogenetic curves of rhizopus nigricans growth at 25 degrees c inhibited by four kinds of heavy metal ions are determined, parameters such as growth rate constants k, inhibitory ratio i, half inhibitory concentration ic50 et al. are obtained. the experimental results show that heavy metal ions can inhibit rhizopus nigricans growth obviously, low concentration of cu2+ has promoting action. the i ... | 1999 | 10903119 |
| biotransformation of methyl ent-17-hydroxy-16beta-kauran-19-oate by rhizopus stolonifer. | methyl ent-17-hydroxy-16beta-kauran-19-oate was fed to a 2-day-old culture of the fungus rhizopus stolonifer, fermenting at room temperature (25 degrees c) in an orbital shaker (21). after 11 days, both broth and mycelia were extracted with ethyl acetate. two novel compounds were isolated from this experiment: methyl ent-9alpha,17-dihydroxy-16beta-kauran-19-oate and methyl ent-7alpha,17-dihydroxy-16beta-kauran-19-oate. their structures were fully confirmed by spectroscopic methods. | 2000 | 10855722 |
| evaluation of the mycological status of luncheon meat with special reference to aflatoxigenic moulds and aflatoxin residues. | the luncheon meat samples analyzed, which were produced locally by the two main luncheon meat producing companies in egypt were relatively highly contaminated either by moulds and yeasts in general, aflatoxigenic species and aflatoxin residues in particular. the most frequently encountered fungi from the samples were yeasts, aspergillus niger, a. flavus, penicillium chrysogenum, rhizopus stolonifer, mucor circinelloides. less common were cladosporium sphaerospermum, alternaria alternata, mycosph ... | 1999 | 10823186 |
| characterization of plasma membrane fraction from filamentous fungus rhizopus nigricans. | in the filamentous fungus rhizopus nigricans a steroid hydroxylating multienzyme system is inducible by progesterone and by several other steroids. the biological signal carried by progesterone might be mediated by receptors, located either in the plasma membrane or inside the cell. to elucidate the first possibility, plasma membrane fraction was examined for the presence of progesterone receptors. the isolation of plasma membrane from fungal homogenate containing different other membranes is di ... | 2000 | 10653169 |
| purification of cytochrome p450 from filamentous fungus rhyzopus nigricans. | it has been reported in our previous studies that steroid hydroxylation system of rhizopus nigricans involves cytochrome p450 and nadph-cytochrome p450 reductase in the electron transport system. both enzymes are membrane bound and are located in the microsomal preparations of progesterone induced fungal mycelia. in order to identify and characterize the cytochrome p450 component of fungal monoxygenase system, microsomal proteins from induced mycelia were subjected to high q anion exchange and m ... | 2000 | 10653160 |
| comparison of two monooxygenase systems with cytochrome p450 in filamentous fungus rhizopus nigricans. | besides the progesterone inducible steroid hydroxylase which catalyses the transformation of progesterone to 11alpha-hydroxyprogesterone the presence of another monooxygenase system in filamentous fungus rhizopus nigricans (r. nigricans)--lanosterol demethylase was confirmed. lanosterol demethylase was not inducible with progesterone. after subcellular fractionation both monooxygenase systems were found in microsomal fraction of fungus r. nigricans. to find out how to differentiate between the h ... | 2000 | 10653158 |
| isolation, partial length sequence and expression of steroid inducible hps 70 gene from rhizopus nigricans. | previous studies have shown that filamentous fungus rhizopus nigricans responds to addition of different steroids into growth medium with induction of hydroxylation system and that some steroids provoke stress response. the purpose of this study was to investigate whether those steroids provoke induction of hsp70 gene(s), well studied markers of stress response in different cells and organisms. the expression studies of fungal hsp70 gene(s) using northern blot analysis showed that fungal hsp70 m ... | 2000 | 10653154 |
| extracellular nuclease from rhizopus stolonifer: purification and characteristics of - single strand preferential - deoxyribonuclease activity. | an extracellular nuclease from rhizopus stolonifer (designated as nuclease rsn) was purified to homogeneity by chromatography on deae-cellulose followed by blue sepharose. the m(r) of the purified enzyme determined by native page was 67¿ omitted¿000 and it is a tetramer and each protomer consists of two unidentical subunits of m(r) 21¿ omitted¿000 and 13¿ omitted¿000. it is an acidic protein with a pi of 4.2 and is not a glycoprotein. the purified enzyme showed an obligate requirement of divalen ... | 1999 | 10594367 |
| induction of hsp70 in the fungus rhizopus nigricans. | we characterised two positive clones, rnh20/2 and rnh2/3, isolated from the cdna library prepared from the fungus rhizopus nigricans previously exposed to heat shock. nucleotide sequences of both cdna clones contained open reading frames for polypeptides with a significant amino acid identity to each other and to cytoplasmic members of hsp70s from different eukaryotic organisms. northern blot analysis, using a fragment of rnh2/3 as a probe, revealed that in the fungus r. nigricans the hsp70 tran ... | 1999 | 10558896 |
| the biotransformation of ent-kaur-16-en-19-oic acid by rhizopus stolonifer. | microbial transformation of ent-kaur-16-en-19-oic acid was carried out with r. stolonifer. after seven days of incubation, two metabolites, ent-7 alpha-hydroxy-kaur-16-en-19-oic acid and ent-12 beta-hydroxy-kaur-9(11),16-dien-19-oic acid, were isolated as a result of hydroxylation and hydroxylation/dehydrogenation, respectively. incubation for 15 days also afforded ent-16 beta,17-dihydroxy-kauran-19-oic acid. the metabolites were identified by spectroscopic methods. | 1999 | 10501025 |
| active site characterization of rnase rs from rhizopus stolonifer: involvement of histidine and lysine in catalysis and carboxylate in substrate binding. | chemical modification studies on purified rnase rs revealed the involvement of a single histidine, lysine and carboxylate residue in the catalytic activity of the enzyme. rna could not protect the enzyme against dep- and tnbs-mediated inactivation whereas, substrate protection was observed in case of edac-mediated inactivation of the enzyme. k(m) and k(cat) values of the partially inactivated enzyme samples suggested that while histidine and lysine are involved in catalysis, carboxylate is invol ... | 1999 | 10434056 |
| [aerosolized recombinant interferon-gamma prevent antigen-induced eosinophil recruitment in guinea pig trachea]. | in order to determine whether interferon-gamma (ifn-gamma) inhibits eosinphil infiltration in the trachea of asthmatic guinea pigs induced by rhizopus nigricans. | 1997 | 10374454 |
| studies on toxigenic fungi in roasted foodstuff (salted seed) and halotolerant activity of emodin-producing aspergillus wentii. | commercial roasted salted peanuts (3% nacl), popcorn (1% nacl), summer-squash (9% nacl), sunflower (3% nacl) and wild-melon (3% nacl) seeds are polluted with fungi, mostly aspergillus flavus, a. niger, penicillium chrysogenum, p. corylophilum and rhizopus stolonifer. contamination of popcorn with the fungi is about 10 times higher than in the other foods. these fungi, common also on unsalted seeds, are significantly inhibited in seeds (30% moisture content) treated with 9-21% nacl. the halotoler ... | 1998 | 9821293 |
| cdna sequence and deduced amino acid sequence of a fungal stress protein induced in rhizopus nigricans by steroids. | cdna clone was isolated from lambdagt11 library prepared from rhizopus nigricans after growing the fungus in the presence of progesterone. northern blot analysis of total rna showed that expression of corresponding mrna was up-regulated in r. nigricans after treatment with different steroids and after exposure of the fungus to heat shock or osmotic stress. sequence analysis revealed an open reading frame for a 364-amino-acid polypeptide. the predicted amino acid sequence exhibited significant si ... | 1998 | 9784403 |
| purification and characterization of nadph-cytochrome p450 reductase from filamentous fungus rhizopus nigricans. | we report here the isolation and partial characterization of a flavoprotein, nadph-cytochrome p450 (cytochrome c) reductase. the enzyme is a part of steroid 11 alpha-hydroxylating system and is associated with the microsomal fraction of the fungus rhizopus nigricans. fungal reductase was solubilized from microsomal membranes with triton x-100 and purified to apparent homogeneity by affinity and high-performance ion-exchange chromatography. a 350-fold purification of the enzyme with specific acti ... | 1998 | 9735172 |
| biological control of major postharvest pathogens on apple with candida sake. | epiphytic microorganisms isolated from apples, pears and the surfaces of apple leaves were screened for antagonistic activity against penicillium expansum (blue-mold), botrytis cinerea (gray-mold) and rhizopus nigricans (rhizopus rot) on apple (malus domestica). a total of 933 bacteria and yeasts were tested in primary screening against p. expansum. ninety-two strains reduced the lesion size on apples by more than 50%, 72 of which were isolated from the surface of apples. for secondary screening ... | 1998 | 9600605 |
| extracellular ribonuclease from rhizopus stolonifer: characteristics of an atypical--guanylic acid preferential--enzyme from ribonuclease t2 family. | an extracellular ribonuclease from rhizopus stolonifer (designated as rnase rs) was purified to homogeneity by chromatography on deae-cellulose followed by cm-cellulose. the mr of the purified enzyme determined by gel filtration and sds-page is 25,000 and 28,200, respectively. rnase rs is a glycoprotein and contains 10.5% neutral sugar. it is an acidic protein with a pi of 5.0 and has a blocked n-terminus. the optimum ph and temperature are 5.5 and 45 degrees c, respectively. rnase rs shows high ... | 1998 | 9528662 |
| equine pulmonary mycosis due to aspergillus niger and rhizopus stolonifer. | invasive pulmonary mycosis caused by aspergillus niger and rhizopus stolonifer is reported in a 2-year-old horse, one of three that died after being housed in a disused, uncleaned stable. lesions were characterized by thrombosis of the blood vessels with haemorrhage and tissue necrosis. fungal hyphae were observed both in thrombosed vessels and in adjacent necrotic tissue. in culture media inoculated with lung samples and samples from the bedding hay, two types of colony were recorded and identi ... | 1997 | 9447480 |
| fungi associated with post-harvest rot of black plum (vitex doniana) in nigeria. | the fungi associated with rot of vitex doniana fruits (blackplum) were isolated and identified. aspergillus niger, botryodiplodia theobromae, candida spp. penicillium chrysogenum, rhizopus stolonifer, fusarium pallidoroseum f. oxysporum and mucor mucedo were the primary rot causing fungi in contrast to cladosporium herbarum and mucor circinelloides which were just present as secondary colonizers. the rot fungi penetrated mainly through wounds and bruises on the surface of fruits. mature green fr ... | 1996 | 9208478 |
| interstitial pneumonitis induced in guinea pigs by the antigens of rhizopus nigricans. | rhizopus nigricans (rn) is one of the most common members of the mucorales that produces opportunistic infections and hypersensitivity states. data concerning experimental induction in guinea pigs of hypersensitivity pneumonitis with a glycoprotein antigen are presented. this antigen was obtained from the mycelial and metabolic products of the cultures and was aerosolized during 12 weeks. the presence of specific antibodies (igg and/or ige) was detected by serological techniques; histopathologic ... | 1997 | 9161935 |
| quality control of two rose bengal and modified drbc and dg18 media. | the effect of storage on the performance of four mycological media, dg18, drbc and two rose bengal agars, one from difco, the other recommended by the swedish standard institution, was investigated. the autoclaved media were stored (+4 degrees c) in the dark for up to 26 weeks. following each storage period, the media were remelted and poured and the plates stored (+4 degrees c) in the dark for a maximum of 8 weeks before inoculation with test microorganisms. all media contained antibiotics. bot ... | 1997 | 9105924 |
| [zygomycosis: review of 16 cases]. | zygomycosis (mucormycosis or phycomycosis) is the infection caused by zygomycetes (non partitioned mycelial fungi, usually saprophytes). all cases of zygomycosis diagnosed in the hospital 12 de octubre in madrid, spain, from 1976 to 1994 were reviewed. | 1996 | 9044638 |
| high incidence of aspergillus flavus and aflatoxins in stored groundnut in ghana and the use of a microbial assay to assess the inhibitory effects of plant extracts on aflatoxin synthesis. | groundnut samples from 21 selected markets in the 10 regions of ghana yielded high levels of the aflatoxigenic fungus aspergillus flavus on half-strength potato dextrose agar. the fungus was associated with 31.7 and 12.8%, respectively, of all damaged and undamaged kernels assayed. only 0.24% of total kernels assayed yielded a. parasiticus. other fungi detected from total kernels assayed were a. niger (34%), a. candidus (1.45%), a. tamarii (3.93%), a. ochraceous (5.26%), fusarium spp. (1.7%) pen ... | 1996 | 8981776 |
| production of useful chemicals from sunflower oil by microbial biotransformation with rhizopus stolonifer nrrl 1478. | a number of microorganisms has been screened for growth on sunflower oil as a sole carbon source for production of useful chemicals. rhizopus stolonifer nrrl 1478 was found to transform the lipid contents of sunflower oil into dodecyl b-d-glucopyranoside and dodecanedioic acid in 15 and 25% yields respectively. the produced compounds were isolated and purified by column chromatography and their chemical identities were established using ms, ir, 1h and 13c nmr spectroscopy. | 1996 | 8934681 |
| microbial hydroxylation of 13-ethyl-17 beta-hydroxy-18,19-dinor-17 alpha-pregn-4-en-20-yn-3-one. | the microbial transformation of the racemic mixture of 13-ethyl-17 beta-hydroxy-18,19-dinor-17 alpha-pregn-4-en-20-yn-3-one (1) was investigated. rhizopus nigricans (as 3.2050), r. arrhizus (as 3.4523), aspergillus niger (as 3.2744), a. ochraceus (as 3.1408), and curvularia lunata (nrrl 4381) transformed 1 into its 10 beta-hydroxy derivative (2) as a major metabolite. biotransformation of 1 by aspergillus ochraceus as 3.1408 afforded 7 beta-hydroxy derivative (3) as the only product. | 1996 | 8837292 |
| occupational asthma in a coal miner. | occupational asthma in coal miners is hardly recognised. a report is presented of a coal miner whose clinical picture suggested a respiratory allergy which occurred only in the mine where he worked. serum specific ige levels, skin tests, and bronchial provocation tests with different commercial extracts showed sensitisation to rhizopus nigricans. rhizopus spp were found inside the mine, as demonstrated by cultures on petri plates. | 1996 | 8795682 |
| residues of dicloran in clingstone peaches after pre- and postharvest application. | trials were carried out over a period of several years in order to test the efficacy of pre- and postharvest treatments against fungi causing postharvest rots of stored clingstone peaches destined to canning. among the fungicides tested, dicloran (2, 6-dichloro-4-nitroaniline) was applied against botrytis cinerea, monilia cinerea and rhizopus stolonifer. the purpose of this work was to determine the residues of this fungicide in fresh, washed, peeled and canned fruits. no relevant data are repor ... | 1996 | 8720094 |
| identification and partial characterization of cytosolic progesterone-binding sites in the filamentous fungus rhizopus nigricans. | progesterone and some other steroids have been shown to induce a steroid 11alpha-hydroxylating enzyme system requiring cytochrome p450 in the filamentous fungus rhizopus nigricans. in the present work, we attempted to find out whether the mycelial cytosol contained progesterone-binding sites (pbs) which could function as receptors for p450-inducing steroids and might, therefore, be included in the induction process. two types of constitutive pbs, pbs-i and pbs-ii, were identified in the cytosol ... | 1996 | 8651707 |
| transmission of the effect of an antifungal agent within a single hypha. | a micro-compartment culture method was devised in which a single hypha of rhizopus stolonifer growing on an agar section traversed an antifungal non-diffusible barrier to another agar section; thus the local environment of the distal or proximal part of the hypha could be controlled independently. the responses in terms of hyphal extension of the test fungus to local application of amphotericin b in this culture system were estimated by using an automatic analysing system. after hyphae had trave ... | 1995 | 8597551 |
| degradation of stachyose, raffinose, melibiose and sucrose by different tempe-producing rhizopus fungi. | forty-six strains of tempe-forming rhizopus species were screened for their ability to grow on raffinose as the sole carbon source. six of the strains showed good growth and sporulation. these isolates were one rhizopus oligosporus, one rhizopus microsporus var. chinensis, three rhizopus oryzae and one rhizopus stolonifer. these six moulds and r. oligosporus strain nrrl 2710 were investigated for their metabolism of the raffinose family of alpha-galactoside carbohydrates. degradation experiments ... | 1995 | 8579835 |
| extracellular production of alpha-rhamnosidase by rhizopus nigricans. | extracellular production of alpha-rhamnosidase [ec 3.2.1.40] from an indigeneous fungal strain of r. nigricans has been demonstrated. the enzyme has been shown to follow michaelis-menten kinetics using p-nitrophenyl-alpha-l-rhamnopyranosid as a substrate. the ph and the temperature optima of the enzyme have been found to be around 6.5 and 60 degrees-80 degrees c respectively. | 1995 | 8557313 |
| comparison of methods for estimating the biomass of three food-borne fungi with different growth patterns. | to evaluate the effectiveness of steps taken to reduce the growth of molds in food and feed, methods that can accurately quantify the degree of fungal contamination of solid substrates are needed. in this study, the ergosterol assay has been evaluated by comparing the results of this assay with spore counts and hyphal length measurements made with a microscope and with cfu counts. three fungi with different growth patterns during cultivation on a synthetic agar substrate were used in these exper ... | 1993 | 8434921 |
| survey of mycoflora and mycotoxins in egyptian soybean seeds. | after four months in commercial storage, 100 soybean samples from different places of egyptian governorates were assayed for filamentous fungal growth at two incubation temperatures (28 and 45 degrees c). 73 species and 8 varieties belonging to 32 genera were isolated by the dilution plate method. at 28 degrees c, the common species were aspergillus flavus, a. fumigatus, a. niger and a. alutaceus, followed by a. terreus, penicillium chrysogenum, p. citrinum, mucor hiemalis, m. racemosus, emerice ... | 1993 | 8271157 |
| filamentous fungi and mycotoxin detected in coconut. | fifty-nine species and one variety belonging to 25 genera of fungi were isolated from 25 coconut samples on glucose-czapek's (25 genera and 55 species + 1 variety) and dichloran-glycerol (8 genera and 32 species + 1 variety) agar media at 28 degrees c. the common fungi on both media used were aspergillus flavus, a. niger, penicillium chrysogenum and cladosporium cladosporioides. on glucose-czapek's agar, a. flavus var. columnaris, p. oxalicum, alternaria alternata, rhizopus stolonifer and tricho ... | 1993 | 8212938 |
| fungal flora and mycotoxins of six kinds of nut seeds for human consumption in saudi arabia. | a wide range of moulds representing several genera and species, was recorded in this study from 5 seed samples of each almond, cashew nut, chestnut, hazelnut, pistachio nut and walnut collected from different markets in ar' ar, saudi arabia. the total counts of fungi were widely fluctuated between 1960-7704 and 1948-7434 colonies/g dry seeds on glucose-czapek's and glycerol agar media at 28 degrees c, respectively, and represented twenty genera, 53 species and 2 varieties of fungi. the prevalent ... | 1993 | 8159218 |
| investigation on the toxicity of fungi from rootstock snacks. | chicken embryo bioassay was used to monitor the toxicity of extracts from rootstock snack samples during a 210-day storage period. results show that the relative toxicity values which were initially very low increased significantly as from 120th day (when 32% mortality was recorded) up till the last day when 73% was obtained. toxicity of extracts from axenic cultures of 12 fungal species isolated from the snack samples was also determined. the strains of aspergillus chevalieri, rhizopus nigrican ... | 1994 | 8145803 |
| synthesis of enantio-manoyl oxides: modifiers of the activity of adenylatecyclase enzyme. | cyclization of methyl ent-8 alpha-hydroxylabd-13(16),14-dien-18-oate with m-chloroperbenzoic acid gave methyl (13s)-ent-16-hydroxy-8 alpha,13-epoxylabd-14-en-18-oate and its epimer at c-13. biotransformation of the former (which exhibits antileishmania activity) with rhizopus nigricans cultures produced the methyl (13s)-ent-11 beta,16-dihydroxy-8 alpha,13-epoxilabd-14-en-18-oate (carbomanoyl, which inhibits the activity of the adenylatecyclase enzyme), methyl (13s)-ent-3 beta,16-dihydroxy-8 alph ... | 1995 | 7772300 |
| the role of cytochrome p450(11 alpha) in detoxification of steroids in the filamentous fungus rhizopus nigricans. | the evidence was presented that steroid hydroxylating enzyme complex induced by substrate in the filamentous fungus rhizopus nigricans (r. nigricans) alleviated toxic effect(s) of the steroid on fungal growth. the growth inhibition of fungal mycelium observed in steroid-containing culture(s) became much more obvious when fungal mycelia were grown in the simultaneous presence of inducing steroid and the p450(11 alpha) inhibitor metyrapone. on the other hand, in experiments where we followed the f ... | 1995 | 7696148 |
| contribution of selected fungi to the reduction of cyanogen levels during solid substrate fermentation of cassava. | the effect of six individual strains of the dominant microflora in solid substrate fermenting cassava on cyanogen levels was examined. six out of eight batches of disinfected cassava root pieces were incubated for 72 h after inoculation with either of the fungi geotrichum candidum, mucor racemosus, neurospora sitophila, rhizopus oryzae and rhizopus stolonifer, or a bacillus sp., isolated from on-farm fermented cassava flours from uganda. one non-inoculated batch was incubated as a reference. lev ... | 1995 | 7577362 |
| studies on fungi associated with tomato fruits and effectiveness of some commercial fungicides against three pathogens. | among 39 fungal species and one variety belonging to 16 genera isolated from 116 healthy tomato fruits collected from markets in assiut, egypt, during 1994, aspergillus niger was found to be the most prevalent, being isolated from 84.6% of the samples. of 11 species recovered from 156 diseased tomatoes, alternaria alternata, rhizopus stolonifer and a. niger were the most common and isolated from 52.7%, 35.9% and 25.0% of the samples, respectively. experiments for comparison of the effects of med ... | 1995 | 7566057 |
| ultrastructure of spores of rhizopus nigricans after repeated freezing and thawing shocks. | disintegration of nuclear envelopes is the only ultrastructural change detectable by freeze-etching in dormant spores of the mould rhizopus nigricans, both dry and swollen, subjected to repeated freezing and thawing. the increase of the number of freeze-inactivated spores corresponds well with the increase of the number of damaged nuclei. this fact led us to formulate a hypothesis that the structure of the nucleus is the primary target of the freezing or thawing damage. as other biomembranes are ... | 1980 | 7399373 |
| aflatoxin and aflatoxicosis. i. fungal flora of some food and animal feeds with special references to aflatoxin-producing abilities. | a wide range of fungi, amounting to fifty-six species belonging to twenty-two genera, have been recovered from animal feeds and foodstuffs. the most frequent fungi were aspergillus niger, and a. flavus, followed by mucor racemosus, alternaria alternata. rhizopus stolonifer, penicillium corylophilum and p. notatum. three genera were found to be of moderate occurrence, namely, mucor, rhizopus and alternaria. the three following genera were of low occurrence: cladosporium, fusarium, and neurospora. ... | 1982 | 7070483 |
| inducibility of cytochrome p-450 and of nadph-cytochrome c reductase in progesterone treated filamentous fungi rhizopus nigricans and rhizopus arrhizus. | 1981 | 6785529 | |
| composition of the fungal flora of four cereal grains in saudi arabia. | using the grain-plate method and on glucose- czapek 's agar at 28 degrees c, fifty-eight species belonging to 26 genera were collected from barley (42 species and 19 genera), maize (29 species and 16 genera), sorghum (32 species and 17 genera) and wheat grains (42 species and 18 genera). the most frequent genera were aspergillus, penicillium, rhizopus, fusarium, and mucor followed by alternaria, drechslera , and curvularia. from the preceding genera aspergillus flavus, a. niger, penicillium nota ... | 1984 | 6727980 |
| d-homoandrostanes. 4. the incubation of some d-homo-5 alpha-androstanes with rhizopus nigricans. | nine dioxygenated d-homoandrostanes were incubated with rhizopus nigricans to investigate the effect of d-ring modification on microbiological hydroxylation. structure determination of the products by nmr spectroscopy, and in certain cases independent synthesis of their oxidised products, showed that in contrast to 5 alpha-androstanes the majority of the compounds were hydroxylated in the "reverse" mode, and only d-homo-5 alpha-androstane-3,17-dione was hydroxylated in the "normal" mode to any e ... | 1983 | 6658861 |
| solubilization and separation of microsomal cytochrome p-450 and nadph-cytochrome c reductase of rhizopus nigricans. | 1983 | 6408312 | |
| substrate effect on 2,3-butylene glycol production by rhizopus nigricans and penicillium expansum. | rhizopus nigricans and penicillium expansum produced 2,3-butylene glycol which accumulated in natural and artificial media with time. mycelial mats of p. expansum decreased the quantity of a diacetyl substrate and converted part of this substrate into acetylmethylcarbinol (amc) and 2,3-butylene glycol. mycelial mats of p. expansum also decreased amc substrate with the formation of 2,3-butylene glycol. 2,3-butylene glycol decreased slightly during incubation with the fungal mat. the formation of ... | 1967 | 6080717 |
| control of malate synthase formation in rhizopus nigricans. | the control of malate synthase formation in a fumaric acid-producing strain of rhizopus nigricans has been found to be similar in most respects to that of isocitrate lyase, the companion enzyme of the glyoxylate bypass. a basal level is formed in a casein hydrolysate medium, which is repressed by glucose. utilization of glucose during growth results in relief of glucose repression. any factor which stimulates growth promotes relief of glucose repression by enhancing the incorporation of represso ... | 1967 | 6074401 |
| mechanism of fumaric acid accumulation in rhizopus nigricans. | it is doubtful that the glyoxylate bypass plays a significant role in the accumulation of fumaric acid by fungi, as has been postulated. in high glucose media, which favor fumarate production, isocitrate lyase (threo-d(s) isocitrate glyoxylate lyase), which is the key enzyme of the glyoxylate bypass, is strongly repressed. the specific activity of this enzyme remains low as long as glucose is present in the medium, even though fumarate formation proceeds at a high level. in addition, the activit ... | 1967 | 6020565 |
| effects of chemical protection on repair of potentially lethal irradiation injury in rhizopus stolonifer sporangiospores. | 1967 | 6019779 | |
| ultrasound and the ultramicroscopic structure of rhizopus nigricans. | 1966 | 5990081 | |
| fungistatic effects of controlled atmospheres. | the fungistatic effects of controlled atmospheres composed of increased co(2) and decreased o(2) was studied in a manner such that the condition of stored fruit was not a factor in the growth of the fungi. varying concentrations of o(2) and co(2) were used. the fungi used were botrytis alli, rhizopus nigricans, and penicillium expansum. the results showed that controlled atmospheres, within the limits of concentrations usable for fruit storage, are effective fungistatic agents. | 1966 | 5951331 |
| inactivation of rhizopus stolonifer sporangiospores by single and combined treatments of heating, chilling, and gamma-irradiation. | 1969 | 5821201 | |
| organization of enzymes in the common aromatic synthetic pathway: evidence for aggregation in fungi. | centrifugation in sucrose density gradients of partially purified extracts from six species of fungi, i.e., rhizopus stolonifer, phycomyces nitens, absidia glauca (phycomycetes), aspergillus nidulans (ascomycetes), coprinus lagopus, and ustilago maydis (basidiomycetes), indicate that the five enzymes catalyzing steps two to six in the prechorismic acid part of the polyaromatic synthetic pathway sediment together. the sedimentation coefficients for these enzymes are very similar in the six specie ... | 1969 | 5802608 |
| ultrastructural changes in rhizopus stolonifer sporangiospores in response to anaerobiosis. | rhizopus stolonifer sporangiospores developed prominent ultrastructural changes under anoxia. mitochondria were scattered randomly throughout the cytoplasm in spores incubated aerobically for 3 hr, but moved to the periphery of the cytoplasm when the spores were exposed to anoxia. endoplasmic reticulum, inconspicuous in control spores, appeared near the plasmalemma and was frequently found connected to a nuclear membrane. the cytoplasm, otherwise usually uniformly dense and containing abundant r ... | 1969 | 5784225 |
| formation and regeneration of protoplasts in the mold rhizopus nigricans. | 1968 | 5672584 | |
| [effect of some cultivation conditions on the ability of mucor fungi tieghemella hyalospora and rhizopus nigricans to effect 11 alpha-hydroxylation of steroid molecules]. | 1971 | 5567625 | |
| [rhizopus nigricans ehrenberg. first results of the physico-chemical and biological study of a culture filtrate]. | 1970 | 5500091 | |
| acidic polysaccharides from the cell wall of absidia cylindrospora, mucor mucedo, and rhizopus nigricans. | 1970 | 5471789 | |
| preliminary characterization of the hemolysin of rhizopus nigricans. | 1970 | 5466030 | |
| hemolytic activity of rhizopus nigricans and rhizopus arrhizus. | 1970 | 5466029 | |
| transformation of progesterone by rhizopus nigricans ref 129 as influenced by modification of the fermentation medium. | 1970 | 5460205 | |
| [method for determination of bacterial mobility by culture on rhizopus nigricans]. | 1970 | 5426400 | |
| transformation of progesterone by aspergillus niger 100 and rhizopus nigricans ref, 129. | 1970 | 5420621 | |
| microbiological transformations. i. hydroxylation of progesterone at c-11 by rhizopus nigricans and penicillium oxalicum. | 1969 | 5380069 | |
| protein synthesis during fungal spore germination. iv. transfer ribonucleic acid from germinated and ungerminated spores. | transfer ribonucleic acid (trna) fractions isolated from germinated and ungerminated spores of botryodiplodia theobromae and rhizopus stolonifer had acceptor activity for all 20 amino acids commonly found in protein, when tested with an enzyme fraction from germinated spores. accordingly, it is unlikely that the absence of trna for a particular amino acid limits protein synthesis in fungal spores. | 1969 | 5364287 |
| pyruvate carboxylase of rhizopus nigricans and its role in fumaric acid production. | 1969 | 5349282 | |
| the in vitro transformation of progesterone by rhizopus nigricans ref 129. | 1971 | 5160388 | |
| [rhizopus nigricans ehrenberg. studies on its nitrogen nutrition; correlative study of the ponderal growth of the mycelium, of the media ph and of the spectrum of culture filtrates as a function of time]. | 1971 | 5149948 | |
| [determination of the bacterial motility rate by cultivation with rhizopus nigricans]. | 1971 | 5146296 | |
| studies on fungal polysaccharides. ix. the acidic polysaccharide from the cell wall of rhizopus nigricans. | 1971 | 5145919 | |
| protein synthesis during fungal-spore germination. vi. analysis of transfer ribonucleic acid from germinated and ungerminated spores of rhizopus stolonifer. | 1972 | 5070713 | |
| changes in soluble ribonucleic acid polymerases associated with the germination of rhizopus stolonifer spores. | 1972 | 5043713 | |
| [hydroxylation of progesterone by rhizopus nigricans in the presence of chloramphenicol]. | 1970 | 4986691 | |
| [hydroxylation of progesterone by rhizopus nigricans]. | 1969 | 4981103 | |
| ultrastructural details in germinating sporangiospores of rhizopus stolonifer and rhizopus arrhizus. | electron microscope examination of sporangiospore sections from rhizopus stolonifer (ehrenb. ex fr.) lind. and r. arrhizus fischer revealed details on intracellular organization not previously reported. aldehyde fixation followed by chromeosmium postfixation permitted clear depiction of ribosomes hitherto unrevealed in these cells. mitochondria were diversiform. spore wall structures in the two species were generally similar, but outer contours differed sufficiently to permit easy species identi ... | 1968 | 4876136 |
| physiological and morphological correlation of rhizopus stolonifer spore germination. | sporangiospores of rhizopus stolonifer were examined at various stages of germination by scanning electron and phase-contrast microscopy. these observations were correlated with changes in spore dry weight, spore volume, respiration, and syntheses of ribonucleic acid, deoxyribonucleic acid, and protein during germination. | 1974 | 4811549 |
| [effect of the incubation temperature on the growth and spore formation of penicillium italicum and rhizopus nigricans treated with ionizing radiations]. | 1973 | 4777355 | |
| lomasome development in rhizopus stolonifer sporangiospores during anaerobiosis. | 1973 | 4752340 | |
| microbiological hydroxylation of steroids. 8. the pattern of monohydroxylation of diketones and keto-alcohols derived from 5 alpha-androstane with cultures of the fungus, rhizopus nigricans. | 1973 | 4739029 | |
| microbiological hydroxylation of steroids. vii. the pattern of dihydroxylation of mono-oxo-5 alpha-androstanes and 5 alpha-estranes with the fungus rhizopus nigricans. | 1973 | 4739028 | |
| characteristics of deoxyribonucleic acid polymerase isolated from spores of rhizopus stolonifer. | deoxyribonucleic acid (dna)-dependent dna polymerase was purified several hundredfold from germinated and ungerminated spores of the fungus rhizopus stolonifer. the partially purified enzymes from both spore stages exhibited identical characteristics; incorporation of [(3)h]deoxythymidine monophosphate into dna required mg(2+), dna, a reducing agent, and the simultaneous presence of deoxyguanosine triphosphate, deoxycytidine triphosphate, and deoxyadenosine triphosphate. heat-denatured and activ ... | 1973 | 4728271 |
| translocation of s 35 in rhizopus stolonifer. | 1972 | 4556581 | |
| [effect of the number of methyl groups in the progesterone molecule on its hydroxylation by rhizopus nigricans cultures]. | 1974 | 4444567 | |
| synthesis of ribonucleic acids during the germination of rhizopus stolonifer sporangiospores. | 1974 | 4435301 |