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mir408 regulates grain yield and photosynthesis via a phytocyanin protein.increasing grain yield is the most important object of crop breeding. here, we report that the elevated expression of a conserved microrna, osmir408, could positively regulate grain yield in rice (oryza sativa) by increasing panicle branches and grain number. we further showed that osmir408 regulates grain yield by down-regulating its downstream target, osucl8, which is an uclacyanin (ucl) gene of the phytocyanin family. the knock down or knock out of osucl8 also increases grain yield, while the ...201728904074
siz1-mediated sumoylation during phosphate homeostasis in plants: looking beyond the tip of the iceberg.availability of phosphate (pi) is often limited in rhizospheres in different agroclimatic zones and adversely affects growth and development of plants. to circumvent this impasse, there is an urgent need and global consensus to develop pi use efficient crops. to achieve this goal, it is essential to identify the molecular entities that exert regulatory influences on the sensing and signaling cascade governing pi homeostasis. siz1 encodes a small ubiquitin-like modifier (sumo e3) ligase, and play ...201728903074
plant stand p-loop ntpases: a current perspective of genome distribution, evolution, and function : plant stand p-loop ntpases: genomic organization, evolution, and molecular mechanism models contribute broadly to plant pathogen defense.stand p-loop ntpase is the common weapon used by plant and other organisms from all three kingdoms of life to defend themselves against pathogen invasion. the purpose of this study is to review comprehensively the latest finding of plant stand p-loop ntpase related to their genomic distribution, evolution, and their mechanism of action. earlier, the plant stand p-loop ntpase known to be comprised of only nbs-lrrs/ap-atpase/nb-arc atpase. however, recent finding suggests that genome of early gree ...201728900732
soil co2 venting as one of the mechanisms for tolerance of zn deficiency by rice in flooded soils.we sought to explain rice (oryza sativa) genotype differences in tolerance of zinc (zn) deficiency in flooded paddy soils and the counter-intuitive observation, made in earlier field experiments, that zn uptake per plant increases with increasing planting density. we grew tolerant and intolerant genotypes in a zn-deficient flooded soil at high and low planting densities and found (a) plant zn concentrations and growth increased with planting density and more so in the tolerant genotype, whereas ...201728898428
transcription factor oswrky53 positively regulates brassinosteroid signaling and plant architecture.brassinosteroids (brs) are a class of steroid hormones regulating multiple aspects of plant growth, development, and adaptation. compared with extensive studies in arabidopsis (arabidopsis thaliana), the mechanism of br signaling in rice (oryza sativa) is less understood. here, we identified oswrky53, a transcription factor involved in defense responses, as an important regulator of rice br signaling. phenotypic analyses showed that oswrky53 overexpression led to enlarged leaf angles and increas ...201728894020
comprehensive survey and evolutionary analysis of genome-wide mirna genes from ten diploid oryza species.micrornas (mirnas) are non-coding rnas that play versatile roles in post-transcriptional gene regulation. although much is known about their biogenesis, and gene regulation very little is known about their evolutionary relation among the closely related species.201728893199
transcription of mcra gene decreases upon prolonged non-flooding period in a methanogenic archaeal community of a paddy-upland rotational field soil.methanogenic archaea survive under aerated soil conditions in paddy fields, and their community is stable under these conditions. changes in the abundance and composition of an active community of methanogenic archaea were assessed by analyzing mcra gene (encoding α subunit of methyl-coenzyme m reductase) and transcripts during a prolonged drained period in a paddy-upland rotational field. paddy rice (oryza sativa l.) was planted in the flooded field and rotated with soybean (glycine max [l.] me ...201728890994
performance of five plant species in removal of nitrogen and phosphorus from an experimental phytoremediation system in the ningxia irrigation area.agricultural non-point source (anps) pollution is an important contributor to elevated nitrogen (n) and phosphorus (p) in surface waters, which can cause serious environmental problems. considerable effort has therefore gone into the development of methods that control the anps input of n and p to surface waters. phytoremediation has been extensively used because it is cost-effective, environmentally friendly, and efficient. the n and p loads from agricultural drainage are a potential threat to ...201728890992
influence of rapeseed cake on iron plaque formation and cd uptake by rice (oryza sativa l.) seedlings exposed to excess cd.a soil spiking experiment at two cd levels (0.72 and 5.20 mg kg-1) was conducted to investigate the effects of rapeseed cake (rsc) at application rates of 0%, 0.75%, 1.5%, and 3.0% (w/w) on iron plaque formation and cd uptake by rice (oryza sativa l.) seedlings. the use of rsc did result in a sharp decrease in soil bioavailability of cd and a significant increase in rice growth, soil ph and organic matter. application of rsc increased the amount of iron plaque formation and this effectively inhi ...201728889221
contribution of dark septate fungi to the nutrient uptake and growth of rice plants.the use of dark septate fungi (dse) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. this study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. four dark septate fungi isolates that were identified by internal transcribed spacer phylogeny were inoculated in rice seeds (cv. piauí). the resulting root colonization was est ...201828888828
improved short-term drought response of transgenic rice over-expressing maize c4 phosphoenolpyruvate carboxylase via calcium signal cascade.to understand the link between long-term drought tolerance and short-term drought responses in plants, transgenic rice (oryza sativa l.) plants over-expressing the maize c4-pepc gene encoding phosphoenolpyruvate carboxylase (pc) and wild-type (wt) rice plants were subjected to peg 6000 treatments to simulate drought stress. compared with wt, pc had the higher survival rate and net photosynthetic rate after 16days of drought treatment, and had higher relative water content in leaves after 2h of d ...201728888162
mapping and comparative proteomic analysis of the starch biosynthetic pathway in rice by 2d page/ms.our results not only provide a comprehensive overview of the starch biosynthetic pathway in the developing endosperm but also reveal some important protein markers that regulate the synthesis of starch. in human diets, rice (oryza sativa l.) is an important source of starch, a substantial amount of which is accumulated in developing endosperm. a better understanding of the complicated pathways involved in starch biosynthesis is needed to improve the yield and quality of rice and other cereal cro ...201728887709
genetic dissection of grain traits in yamadanishiki, an excellent sake-brewing rice cultivar.the grain traits of yamadanishiki, an excellent sake-brewing rice cultivar in japan, are governed by multiple qtls, namely, a total of 42 qtls including six major qtls. japanese rice wine (sake) is produced using brewing rice (oryza sativa l.) that carries traits desirable for sake-brewing, such as a larger grain size and higher white-core expression rate (wce) compared to cooking rice cultivars. however, the genetic basis for these traits in brewing rice cultivars is still unclear. we performed ...201728887658
a rice genetic improvement boom by next generation sequencing.rice (oryza sativa l.) is a staple food crop for people worldwide, and a key goal has been to increase its grain yield. an increasing population that relies on a decreasing level of farmland has rendered the traditional method for the isolation and use of genetic loci in rice breeding unsatisfactory. recently, the rapid development in next generation sequencing (ngs) has boosted the number of genome sequences for hundreds to thousands of rice varieties. a mutmap strategy and bulk segregation ana ...201728885178
efficient crispr/cas9-mediated genome editing using a chimeric single-guide rna molecule.the crispr/cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. however, targeted gene editing is inefficient and requires the simultaneous delivery of a dna template for homology-directed repair (hdr). here, we used crispr/cas9 to generate targeted double-strand breaks and to deliver an rna repair template for hdr in rice (oryza sativa). we used chimeric single-guide rna (cgrna) molecules carrying both sequences for target site specificity (to generate the doub ...201728883826
malonylome analysis in developing rice (oryza sativa) seeds suggesting that protein lysine malonylation is well-conserved and overlaps with acetylation and succinylation substantially.in recent years, lysine malonylation has garnered wide spread interest due to its potential regulatory roles. while studies have been performed in bacteria, mouse, and human, the involvement and the biological function of this modification in plant are still largely unknown. we examined the global proteome profile of lysine malonylation in developing rice seeds using affinity enrichment followed by lc-ms/ms analysis. we identified 421 malonylated lysine sites across 247 proteins. functional anal ...201828882676
isolation and identification of five cold-inducible promoters from oryza sativa.five promoters of the cold-inducible rice genes were isolated. the quantitative and qualitative expression analyses in the high generation transgenic rice suggest that the genes are stably induced by low temperature. cold-inducible promoters are highly desirable for stress-inducible gene expression in crop genetic engineering. in this study, five rice genes, including osaba8ox1, osmyb1r35, oserf104, oscyp19-4, and osabcb5, were found to be transcriptionally induced by cold stress. the promoters ...201828879616
mutations in the gene of the gα subunit of the heterotrimeric g protein are the cause for the brachytic1 semi-dwarf phenotype in barley and applicable for practical breeding.short-culm mutants have been widely used in breeding programs to increase lodging resistance. in barley (hordeum vulgare l.), several hundreds of short-culm mutants have been isolated over the years. the objective of the present study was to identify the brachytic1 (brh1) semi-dwarfing gene and to test its effect on yield and malting quality.201828878591
genome-wide analysis and expression profiling of zinc finger homeodomain (zhd) family genes reveal likely roles in organ development and stress responses in tomato.zinc finger homeodomain proteins (zhd) constitute a plant-specific transcription factor family with a conserved dna binding homeodomain and a zinc finger motif. members of the zhd protein family play important roles in plant growth, development, and stress responses. genome-wide characterization of zhd genes has been carried out in several model plants, including arabidopsis thaliana and oryza sativa, but not yet in tomato (solanum lycopersicum).201728874115
degrading and phytoextracting atrazine residues in rice (oryza sativa) and growth media intensified by a phase ii mechanism modulator.atrazine (atz) residue in farmland is one of the environmental contaminants seriously affecting crop production and food safety. understanding the regulatory mechanism for atz metabolism and degradation in plants is important to help reduce atz potential toxicity to both plants and human health. here, we report our newly developed engineered rice overexpressing a novel phase ii metabolic enzyme glycosyltransfearse1 (argt1) responsible for transformation of atz residues in rice. our results showe ...201728872855
micrornas regulate the main events in rice drought stress response by manipulating the water supply to shoots.micrornas (mirnas) are small endogenous regulatory rnas that are involved in a variety of biological processes related to proliferation, development, and response to biotic and abiotic stresses. mirna profiles of rice (oryza sativa l. cv. ir64.) leaves in a partial root zone drying (prd) system were analysed using a high-throughput sequencing approach to identify mirnas associated with drought signalling. the treatments performed in this study were as follows: well-watered ("wet" roots, ww), whe ...201728872648
the iron-chelate transporter osysl9 plays a role in iron distribution in developing rice grains.rice osysl9 is a novel transporter for fe(ii)-nicotianamine and fe(iii)-deoxymugineic acid that is responsible for internal iron transport, especially from endosperm to embryo in developing seeds. metal chelators are essential for safe and efficient metal translocation in plants. graminaceous plants utilize specific ferric iron chelators, mugineic acid family phytosiderophores, to take up sparingly soluble iron from the soil. yellow stripe 1-like (ysl) family transporters are responsible for tra ...201728871478
a chemical-induced, seed-soaking activation procedure for regulated gene expression in rice.inducible gene expression has emerged as a powerful tool for plant functional genomics. the estrogen receptor-based, chemical-inducible system xve has been used in many plant species, but the limited systemic movement of inducer β-estradiol in transgenic rice plants has prohibited a wide use of the xve system in this important food crop. here, we constructed an improved chemical-regulated, site-specific recombination system by employing the xve transactivator in combination with a cre/loxp-frt s ...201728871269
molecular mapping of qtls associated with lodging resistance in dry direct-seeded rice (oryza sativa l.).dry direct-seeded rice (dsr) is an alternative crop establishment method with less water and labor requirement through mechanization. it provides better opportunities for a second crop during the cropping season and therefore, a feasible alternative system to transplanted lowland rice. however, lodging is one of the major constraints in attaining high yield in dsr. identification of qtls for lodging resistance and their subsequent use in improving varieties under dsr will be an efficient breedin ...201728871266
integrating early transcriptomic responses to rhizotoxins in rice (oryza sativa. l.) reveals key regulators and a potential early biomarker of cadmium toxicity.as sessile organisms, plants were constantly challenged with biotic and abiotic stresses. transcriptional activation of stress-responsive genes is a crucial part of the plant adaptation to environmental changes. here, early response of rice root to eight rhizotoxic stressors: arsenate, copper, cadmium, mercury, chromate, vanadate, ferulic acid and juglone, was analyzed using published microarray data. there were 539 general stress response (gsr) genes up-regulated under all eight treatments, inc ...201728868059
effects of modified biochar on rhizosphere microecology of rice (oryza sativa l.) grown in as-contaminated soil.biochar was carbon-rich and generated by high-temperature pyrolysis of biomass under oxygen-limited conditions. due to the limitations of surface functional groups and the weakness of surface activity in the field of environmental remediation, the raw biochar frequently was chemically modified to improve its properties with a new performance. in this study, a kind of high-efficiency and low-cost amino biochar modified by nano zero-valent iron (abc/nzvi) was synthesized and applied to paddy soil ...201728866780
effects of ethylene and gibberellins on the elongation of rice seedlings (oryza sativa l.).three-day-old rice seedlings treated with ethylene showed elongation of the 2nd and 3rd leaves. this ethylene-stimulated elongation was not observed in the presence of uniconazole-p or prohexadione, both gibberellin (ga) biosynthesis inhibitors, suggesting that ga was involved in the response. an analysis of endogenous gas by gc-ms revealed that the ga1 level was reduced in the 3rd leaf in response to ethylene. dose-response experiments showed that the responsiveness to ga1 was enhanced by ethyl ...199728862568
identification and fine mapping of a new gene, bph31 conferring resistance to brown planthopper biotype 4 of india to improve rice, oryza sativa l.rice (oryza sativa l.) is the staple food for more than 3.5 billion people, mainly in asia. brown planthopper (bph) is one of the most destructive insect pests of rice that limits rice production. host-plant resistance is one of the most efficient ways to overcome bph damage to the rice crop.201728861736
mapk kinase 10.2 promotes disease resistance and drought tolerance by activating different mapks in rice.mitogen-activated protein kinase (mapk) cascades, with each cascade consisting of a mapk kinase kinase (mapkkk), a mapk kinase (mapkk), and a mapk, have important roles in different biological processes. however, the signal transduction in rice mapk cascades remains to be elucidated. we show that the structural non-canonical mapkk, mpkk10.2, enhances rice resistance to xanthomonas oryzae pv. oryzicola (xoc), which causes bacterial streak disease, and increases rice tolerance to drought stress by ...201728857351
evolutionary modes of emergence of short interspersed nuclear element (sine) families in grasses.short interspersed nuclear elements (sines) are non-autonomous transposable elements which are propagated by retrotransposition and constitute an inherent part of the genome of most eukaryotic species. knowledge of heterogeneous and highly abundant sines is crucial for de novo (or improvement of) annotation of whole genome sequences. we scanned poaceae genome sequences of six important cereals (oryza sativa, triticum aestivum, hordeum vulgare, panicum virgatum, sorghum bicolor, zea mays) and bra ...201728857316
large-scale deployment of a rice 6 k snp array for genetics and breeding applications.fixed arrays of single nucleotide polymorphism (snp) markers have advantages over reduced representation sequencing in their ease of data analysis, consistently higher call rates, and rapid turnaround times. a 6 k snp array represents a cost-benefit "sweet spot" for routine genetics and breeding applications in rice. selection of informative snps across species and subpopulations during chip design is essential to obtain useful polymorphism rates for target germplasm groups. this paper summarize ...201728856618
plant glyoxylate/succinic semialdehyde reductases: comparative biochemical properties, function during chilling stress, and subcellular localization.plant nadph-dependent glyoxylate/succinic semialdehyde reductases 1 and 2 (cytosolic glyr1 and plastidial/mitochondrial glyr2) are considered to be of particular importance under abiotic stress conditions. here, the apple (malus × domestica borkh.) and rice (oryza sativa l.) glyr1s and glyr2s were characterized and their kinetic properties were compared to those of previously characterized glyrs from arabidopsis thaliana [l.] heynh. the purified recombinant glyrs had an affinity for glyoxylate a ...201728855911
large-scale phenomics analysis of a t-dna tagged mutant population.rice, oryza sativa l., is one of the most important crops in the world. with the rising world population, feeding people in a more sustainable and environmentally friendly way becomes increasingly important. therefore, the rice research community needs to share resources to better understand the functions of rice genes that are the foundation for future agricultural biotechnology development, and one way to achieve this goal is via the extensive study of insertional mutants. we have constructed ...201728854617
dissecting combining ability effect in a rice ncii-iii population provides insights into heterosis in indica-japonica cross.combining ability is a measure for selecting elite parents that make the highest contributions to hybrid performance. however, the genetic bases of combining ability and how they contributed to heterosis is seldomly known.201728853048
influence of bacterial strains on oryza sativa grown under arsenic tainted soil: accumulation and detoxification response.the present study was conducted to study the role of bacterial inoculants in growth, accumulation and tolerance responses in rice grown in arsenic (as) contaminated soil. results revealed that out of five isolated bacterial strains, strain bbau/mmm1 (babasaheb bhimrao ambedkar university/mari matamandir) exhibited resistant to as(iii) to the level of 400 μm as(iii) in comparison to other strain which showed toxicity. the isolated strain bbau/mmm1 1was characterized as gram negative, rod shape, s ...201728850869
macromolecular traits in the african rice oryza glaberrima and in glaberrima/sativa crosses, and their relevance to processing.molecular properties of proteins and starch were investigated in 2 accessions of oryza glaberrima and oryza sativa, and in one nerica cross between the 2 species, to assess traits that could be relevant to transformation into specific foods. protein nature and organization in o. glaberrima were different from those in o. sativa and in nerica. despite the similar cysteine content in all samples, thiol accessibility in o. glaberrima proteins was higher than in nerica or in o. sativa. inter-protein ...201728850662
limitation of unloading in the developing grains is a possible cause responsible for low stem non-structural carbohydrate translocation and poor grain yield formation in rice through verification of recombinant inbred lines.remobilisation of non-structural carbohydrates (nsc) from leaves and stems and unloading into developing grains are essential for yield formation of rice. in present study, three recombinant inbred lines of rice, r91, r156 and r201 have been tested for source-flow-sink related attributes determining the nature of nsc accumulation and translocation at two nitrogen levels in the field. compared to r91 and r156, r201 had lower grain filling percentage, harvest index, and grain yield. meanwhile, r20 ...201728848573
regulation of rice root development by a retrotransposon acting as a microrna sponge.it is well documented that transposable elements (tes) can regulate the expression of neighbouring genes. however, their ability to act in trans and influence ectopic loci has been reported rarely. we searched in rice transcriptomes for tissue-specific expression of tes and found them to be regulated developmentally. they often shared sequence homology with co-expressed genes and contained potential microrna-binding sites, which suggested possible contributions to gene regulation. in fact, we ha ...201728847366
genome-wide analysis of duf221 domain-containing gene family in oryza species and identification of its salinity stress-responsive members in rice.duf221 domain-containing genes (ddp genes) play important roles in developmental biology, hormone signalling transduction, and responses to abiotic stress. therefore to understand their structural and evolutionary relationship, we did a genome-wide analysis of this important gene family in rice. further, through comparative genomics, ddp genes from oryza sativa subsp. (indica), nine different wild species of rice and arabidopsis were also identified. we also found an expansion of the ddp gene fa ...201728846681
mitigation of cd accumulation in paddy rice (oryza sativa l.) by fe fertilization.cadmium uptake in rice is believed to be mediated by the fe transport system. phyto-available cd can be changed by fe fertilization of substrates. this work investigated whether and how fe fertilization affects mitigation of cd accumulation in paddy rice. a 90-d soil column experiment was conducted to study the change of cd and fe availability in soil after fe fertilization (ionic and chelated fe). a low-cd accumulating cultivar (ty116) and a high-cd accumulating cultivar (jy841) were grown in t ...201728843203
regulatory network and genetic interactions established by osmads34 in rice inflorescence and spikelet morphogenesis.grasses display highly diversified inflorescence architectures that differ in the arrangement of spikelets and flowers and determine cereal yields. however, the molecular basis underlying grass inflorescence morphogenesis remains largely unknown. here we investigate the role of a functionally diversified sepallata mads-box transcription factor, osmads34, in regulating rice (oryza sativa l.) inflorescence and spikelet development. microarray analysis showed that, at the very early stages of inflo ...201728843032
reduced arsenic accumulation in indica rice (oryza sativa l.) cultivar with ferromanganese oxide impregnated biochar composites amendments.the effects of biochar (bc) and ferromanganese oxide biochar composites (fmbc1 and fmbc2) on as (arsenic) accumulation in rice were determined using a pot experiment. treatments with bc or fmbc improved the dry weights of rice roots, stems, leaves, and grains in soils containing different as contamination levels. compared to bc treatment, fmbc treatments significantly reduced as accumulation in different parts of the rice plants (p < 0.05), and fmbc2 performed better than fmbc1 did. furthermore, ...201728841500
polyphasic characterization of bacteria obtained from upland rice cultivated in cerrado soil.this work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16s rrna sequencing) and plant growth promotion. results showed a great morphological, metabolic and genetic variability among bacterial isolates. all isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, c ...201728838812
whole genome sequencing and comparative transcriptome analysis of a novel seawater adapted, salt-resistant rice cultivar - sea rice 86.rice (oryza sativa) is critical for human nutrition worldwide. due to a growing population, cultivars that produce high yields in high salinity soil are of major importance. here we describe the discovery and molecular characterization of a novel sea water adapted rice strain, sea rice 86 (sr86).201728835208
gene flow from single and stacked herbicide-resistant rice (oryza sativa): modeling occurrence of multiple herbicide-resistant weedy rice.provisia™ rice (pv), a non-genetically engineered (ge) quizalofop-resistant rice, will provide growers with an additional option for weed management to use in conjunction with clearfield® rice (cl) production. modeling compared the impact of stacking resistance traits versus single traits in rice on introgression of the resistance trait to weedy rice (also called red rice). common weed management practices were applied to 2-, 3- and 4-year crop rotations, and resistant and multiple-resistant wee ...201828834651
genome-wide identification and characterization of the gmsnrk2 family in soybean.sucrose non-fermenting-1 (snf1)-related protein kinase 2s (snrk2s) that were reported to be involved in the transduction of abscisic acid (aba) signaling, play important roles in response to biotic and abiotic stresses in plants. compared to the systemic investigation of snrk2s in arabidopsisthaliana and oryza sativa, little is known regarding snrk2s in soybean, which is one of the most important oil and protein crops. in the present study, we performed genome-wide identification and characteriz ...201728832544
transcriptional regulatory network of wox11 is involved in the control of crown root development, cytokinin signals, and redox in rice.the rice root system is mainly composed of post-embryonic shoot-borne roots called crown roots. wox11, encoding a wuschel-related homeobox domain transcription factor, is a key regulator of crown root growth and development in rice (oryza sativa. l). in addition to specifically activating crown root development, wox11 is also involved in lateral root initiation, root hair formation, and abiotic stresses. however, the gene regulatory network downstream of wox11 remains largely unknown. here, we s ...201728830102
modulation of aba signaling by altering vxgφl motif of pp2cs in oryza sativa.the abscisic acid (aba) signaling pathway is regulated by clade a type 2c protein phosphatases (pp2cas) in plants. in the presence of aba, pp2cs release stress/aba-activated protein kinases by binding to aba-bound receptors (pyl/rcars) for activation. although the wedging tryptophan in pp2cs is critical in the interaction with pyl/rcars in arabidopsis and rice, it remains elusive as to how other interface regions are involved in the interaction. here, we report the identification of a conserved ...201728827170
field experiment for determining lead accumulation in rice grains of different genotypes and correlation with iron oxides deposited on rhizosphere soil.paddy rice (oryza sativa l.) is a major staple crop in asia. however, heavy metal accumulation in paddy soil poses a health risk for rice consumption. although plant uptake of pb is usually low, pb concentrations in rice plants have been increasing with pb contamination in paddy fields. it is known that iron oxide deposits in the rhizosphere influence the absorption of soil pb by rice plants. in this study, 14 rice cultivars bred in taiwan, including ten japonica cultivars (hl21, kh145, tc192, t ...201828826122
stomatal development: focusing on the grasses.the development and patterning of stomata in the plant epidermis has emerged as an ideal system for studying fundamental plant developmental processes. over the past twenty years most studies of stomata have used the model dicotyledonous plant arabidopsis thaliana. however, cultivated monocotyledonous grass (or gramineae) varieties provide the majority of human nutrition, and future research into grass stomata could be of critical importance for improving food security. recent studies using brac ...201728826033
metals induce transient folding and activation of the twister ribozyme.twister is a small ribozyme present in almost all kingdoms of life that rapidly self-cleaves in variety of divalent metal ions. we used activity assays, bulk fret and single-molecule fret (smfret) to understand how different metal ions promote folding and self-cleavage of the oryza sativa twister ribozyme. although most ribozymes require additional mg2+ for catalysis, twister inverts this expectation, requiring 20-30 times less mg2+ to self-cleave than to fold. transition metals such as co2+, ni ...201728825710
gaba accretion reduces lsi-1 and lsi-2 gene expressions and modulates physiological responses in oryza sativa to provide tolerance towards arsenic.gaba counteracts wide range of stresses through regulation of gaba shunt pathway in plants. although, gaba assisted tolerance against as toxicity in plants is still unexplored. we have examined gaba induced tolerance in rice seedlings with two exposure periods of gaba i.e., short term and long term. results showed that accumulation of gaba reduced the expressions of lsi-1 and lsi-2 transporter genes, which ultimately decreased the accumulation of as in rice seedlings. the accumulation of gaba al ...201728821860
lazy genes mediate the effects of gravity on auxin gradients and plant architecture.a rice (oryza sativa) mutant led to the discovery of a plant-specific lazy1 protein that controls the orientation of shoots. arabidopsis (arabidopsis thaliana) possesses six lazy genes having spatially distinct expression patterns. branch angle phenotypes previously associated with single lazy genes were here studied in roots and shoots of single and higher-order atlazy mutants. the results identify the major contributors to root and shoot branch angles and gravitropic behavior of seedling hypoc ...201728821594
genetic dissection of seedling stage salinity tolerance in rice using introgression lines of a salt tolerant landrace nona bokra.salinity is an important abiotic stress affecting rice production worldwide. development of salt tolerant varieties is the most feasible approach for improving rice productivity in salt affected soils. in rice, seedling stage salinity tolerance is crucial for better crop establishment. quantitative trait loci (qtl) mapping using introgression lines (ils) is useful for identification and simultaneous transfer of desirable alleles into elite genetic background. in the present study, 138 ils derive ...201728821187
rice peroxisomal ascorbate peroxidase knockdown affects ros signaling and triggers early leaf senescence.h2o2, which is continually produced by aerobic metabolism, is a cytotoxic molecule when in high levels. however, low levels can act as a signaling molecule able to regulate the expression of stress responses, senescence, programmed cell death, plant growth, and development. ascorbate peroxidase (apx) enzyme plays an essential role in the control of intracellular h2o2 levels. here, the function of a gene encoding a peroxisomal apx (osapx4) from rice (oryza sativa l.) was studied. osapx4 gene expr ...201728818384
functional characterization of rice cw-domain containing zinc finger proteins involved in histone recognition.histone recognition is important for understanding the mechanisms of histone modification, which play a pivotal role in transcriptional regulation during plant development. here, we identified three cysteine-tryptophan (cw)-domain containing zinc finger (zf) proteins involved in histone recognition, namely oscw-zf3, oscw-zf5 and oscw-zf7. protein sequence analysis showed that they have two unknown motifs in addition to the cw domain. all three oscw-zfs were expressed in aerial tissues, with rela ...201728818372
genome wide association analysis of cold tolerance at germination in temperate japonica rice (oryza sativa l.) varieties.a pool of 200 traditional, landraces and modern elite and old cultivars of rice, mainly japonica varieties adapted to temperate regions, have been used to perform a genome wide association study to detect chromosome regions associated to low temperature germination (ltg) regulation using a panel of 1672 snp markers. phenotyping was performed by determining growth rates when seeds were germinated at 25° and 15°c in order to separate the germination vigorousness from cold tolerance effects. as exp ...201728817683
comparative studies on tolerance of rice genotypes differing in their tolerance to moderate salt stress.moderate salt stress, which often occurs in most saline agriculture land, suppresses crop growth and reduces crop yield. rice, as an important food crop, is sensitive to salt stress and rice genotypes differ in their tolerance to salt stress. despite extensive studies on salt tolerance of rice, a few studies have specifically investigated the mechanism by which rice plants respond and tolerate to moderate salt stress. two rice genotypes differing in their tolerance to saline-alkaline stress, don ...201728814283
rapid and targeted introgression of fgr gene through marker-assisted backcrossing in rice (oryza sativa l.).while it is crucial for developing countries like malaysia to achieve self-sufficiency in rice (oryza sativa l.), it is equally critical to be able to produce high-quality rice, specifically fragrant rice, which demands are often met through importation. the present study was aimed at developing high-yielding fragrant rice, in a timely and cost-effective manner. a marker-assisted backcross (mabc) approach was optimised to introgress the fragrance gene (fgr) into two high-yielding malaysian varie ...201728813631
association analysis of arsenic-induced straighthead in rice (oryza sativa l.) based on the selected population with a modified model.a rice physiological disorder makes mature panicle keep erect with empty grains termed as "straighthead." straighthead causes yield losses and is a serious threat to rice production worldwide. here, a new study of association mapping was conducted to identify qtl involved in straighthead. a subset of 380 accessions was selected from the usda rice core collection and genotyped with 72 genome-wide ssr markers. an optimal model implemented with principle components (pcs) was used in this associatio ...201728812021
crosstalk between diurnal rhythm and water stress reveals an altered primary carbon flux into soluble sugars in drought-treated rice leaves.plants retain rhythmic physiological responses when adapting to environmental challenges. however, possible integrations between drought conditions and those responses have not received much focus, especially regarding crop plants, and the relationship between abiotic stress and the diurnal cycle is generally not considered. therefore, we conducted a genome-wide analysis to identify genes showing both diurnal regulation and water-deficiency response in rice (oryza sativa). among the 712 drought- ...201728811563
the conserved and unique genetic architecture of kernel size and weight in maize and rice.maize (zea mays) is a major staple crop. maize kernel size and weight are important contributors to its yield. here, we measured kernel length, kernel width, kernel thickness, hundred kernel weight, and kernel test weight in 10 recombinant inbred line populations and dissected their genetic architecture using three statistical models. in total, 729 quantitative trait loci (qtls) were identified, many of which were identified in all three models, including 22 major qtls that each can explain more ...201728811335
cropping systems affect paddy soil organic carbon and total nitrogen stocks (in rice-garlic and rice-fava systems) in temperate region of southern china.the accumulation of soil organic carbon (soc) in agricultural soils is critical to food security and climate change. however, there is still limited information on the dynamic trend of soc sequestration following changes in cropping systems. paddy soils, typical of temperate region of southern china, have a large potential for carbon (c) sequestration and nitrogen (n) fixation. it is of great importance to study the impacts of changes in cropping systems on stocks of soc and total nitrogen (tn) ...201728810521
identification and expression analysis of micrornas during ovule development in rice (oryza sativa) by deep sequencing.microrna (mirna) expression profiles during rice ovule development revealed the possible mirna-mediated regulation between ovule sporophytic tissue and female gametophyte and the involvement of mirnas in programmed cell death. mirnas are 20-24-nucleotide small rnas that play key roles in the regulation of many growth and developmental processes in plants. rice ovule development comprises a series of biological events, which are regulated by complex molecular mechanisms. to gain insight into mirn ...201728808767
roles of rice phytochrome-interacting factor-like1 (ospil1) in leaf senescence.rice (oryza sativa) phytochrome-interacting factor-like1 (ospil1), a basic helix-loop-helix transcription factor, plays an important role in the elongation of internode cells. recently, we found that ospil1 participates in chlorophyll biosynthesis by directly upregulating several genes encoding components of the photosystem apparatus. here, we show that ospil1 negatively regulates leaf senescence in rice. during dark-induced senescence (dis), ospil1 mutants senesced earlier than wild type; this ...201728805492
three representative inter and intra-subspecific crosses reveal the genetic architecture of reproductive isolation in rice.systematic characterization of genetic and molecular mechanisms in the formation of hybrid sterility is of fundamental importance in understanding reproductive isolation and speciation. using ultra-high-density genetic maps, 43 single-locus quantitative trait loci (qtls) and 223 digenic interactions for embryo-sac, pollen, and spikelet fertility are depicted from three crosses between representative varieties of japonica and two varietal groups of indica, which provide an extensive archive for i ...201728805257
root transcriptomic analysis revealing the importance of energy metabolism to the development of deep roots in rice (oryza sativa l.).drought is the most serious abiotic stress limiting rice production, and deep root is the key contributor to drought avoidance. however, the genetic mechanism regulating the development of deep roots is largely unknown. in this study, the transcriptomes of 74 root samples from 37 rice varieties, representing the extreme genotypes of shallow or deep rooting, were surveyed by rna-seq. the 13,242 differentially expressed genes (degs) between deep rooting and shallow rooting varieties (h vs. l) were ...201728798764
c-terminal residues of rice translin are essential for octamer formation and nucleic acid binding.translin is a dna/rna binding protein involved in dna repair and rna metabolism. previously, we had shown that rice translin (221 amino acids) exhibits biochemical activities similar to that of the human translin protein. here we report the role of the c-terminal random coil in rice translin function by analyzing truncation (after 215th residue, tra - 215) and substitution mutant proteins (ser216ala, lys217ala, gln218ala, glu219ala). circular dichroism (cd) analysis of tra-215 showed deviations ...201728797959
genome-wide screening of oryza sativa ssp. japonica and indica reveals a complex family of proteins with ribosome-inactivating protein domains.ribosome-inactivating proteins (rips) are cytotoxic enzymes capable of halting protein synthesis by irreversible modification of ribosomes. although rips are widespread they are not ubiquitous in the plant kingdom. the physiological importance of rips is not fully elucidated, but evidence suggests a role in the protection of the plant against biotic and abiotic stresses. searches in the rice genome revealed a large and highly complex family of proteins with a rip domain. a comparative analysis r ...201728797946
how rice (oryza sativa l.) responds to elevated as under different si-rich soil amendments.several strategies exist to mitigate as impacts on rice and each has its set of trade-offs with respect to yield, inorganic as content in grain, and ch4 emissions. the addition of si to paddy soil can decrease as uptake by rice but how rice will respond to elevated as when soil is amended with si-rich materials is unresolved. here, we evaluated yield impacts and grain as content and speciation in rice exposed to elevated as in response to different si-rich soil amendments including rice husk, ri ...201728795805
development of 25 near-isogenic lines (nils) with ten bph resistance genes in rice (oryza sativa l.): production, resistance spectrum, and molecular analysis.a first set of 25 nils carrying ten bph resistance genes and their pyramids was developed in the background of indica variety ir24 for insect resistance breeding in rice. brown planthopper (nilaparvata lugens stal.) is one of the most destructive insect pests in rice. development of near-isogenic lines (nils) is an important strategy for genetic analysis of brown planthopper (bph) resistance (r) genes and their deployment against diverse bph populations. a set of 25 nils with 9 single r genes an ...201728795219
rna-seq data of oryza sativa cultivar kuku belang under peg treatment.drought stress is the main abiotic factor affecting rice production. rain-fed upland rice which is grown on unbounded fields and totally dependent on rainfall for moisture is more prone to drought stress compared to rice from other ecosystems. however, upland rice has adapted to this limited water condition, thus are more drought tolerant than rice from other ecosystems. we performed the first transcriptome sequencing of drought tolerant indica upland rice cultivar kuku belang to identify differ ...201728795103
catalytic and functional aspects of different isozymes of glycolate oxidase in rice.glycolate oxidase (glo) is a key enzyme for photorespiration in plants. there are four glo genes encoding and forming different isozymes in rice, but their functional differences are not well understood. in this study, enzymatic and physiological characteristics of the glo isozymes were comparatively analyzed.201728789632
identification and molecular mapping of indica high-tillering dwarf mutant htd4, a mild phenotype allelic mutant of d14 in rice (oryza sativa l.).metabolism of strigolactones (sls) can improve the efficiency of nutrient use by regulating the development of roots and shoots in crops, making them an important research focus for molecular breeding. however, as a very important plant hormone, the molecular mechanism of sl signal transduction still remains largely unknown. in this study, we isolated an indica high-tillering dwarf mutant 4 (htd4), a spontaneous mutant of rice, from the restorer line gui99. mapping and sequencing analysis showed ...201728787541
identification of the actual coding region for polyamine oxidase 6 from rice (ospao6) and its partial characterization.polyamines (pa) in plant play roles in growth and development and in responses to environmental stresses. the family of polyamine oxidases (pao) contributes to a balanced homeostasis of pas catalyzing two different reactions, terminal catabolic (tc) and back-conversion (bc) pathway, in pa catabolism. from the seven paos encoded by the rice genome (ospao1 - ospao7) ospao6 could so far not be characterized due to failure in obtaining the coding cdna based on accessions in the genomic databases. we ...201728786735
quantitation of glutathione s-transferases in rice (oryza sativa l.) roots exposed to cadmium by liquid chromatography-tandem mass spectrometry using isotope-labeled wing peptides as an internal standard.plant glutathione s-transferases (gsts, ec 2.5.1.18) are multifunctional enzymes involved in heavy metal cellular detoxification by conjugating the tripeptide (g-glu-cys-gly) glutathione to heavy metals. previous studies demonstrated that individual rice gsts were differentially induced by heavy metal exposure at the mrna transcript level. however, little information is available concerning changes in protein concentration of rice gsts under heavy metal stress. because the correlation between ch ...201728785299
microct reveals domesticated rice (oryza sativa) within pottery sherds from early neolithic sites (4150-3265 cal bp) in southeast asia.rice (oryza sativa) was domesticated in the yangtze valley region at least 6000-8000 years ago, yet the timing of dispersal of domesticated rice to southeast asia is contentious. often rice is not well-preserved in archaeobotanical assemblages at early neolithic sites in the wet tropics of southeast asia and consequently rice impressions in pottery have been used as a proxy for rice cultivation despite their uncertain taxonomic and domestication status. in this research, we use microct technolog ...201728785094
polymeric nanoparticles as a metolachlor carrier: water-based formulation for hydrophobic pesticides and absorption by plants.pesticide formulation is highly desirable for effective utilization of pesticide and environmental pollution reduction. studies of pesticide delivery system such as microcapsules are developing prosperously. in this work, we chose polymeric nanoparticles as a pesticide delivery system and metolachlor was used as a hydrophobic pesticide model to study water-based mpeg-plga nanoparticle formulation. preparation, characterization results showed that the resulting nanoparticles enhanced "water solub ...201728783335
the polyketide synthase ospks2 is essential for pollen exine and ubisch body patterning in rice.lipid and phenolic metabolism are important for pollen exine formation. in arabidopsis, polyketide synthases (pkss) are essential for both sporopollenin biosynthesis and exine formation. here, we characterized the role of a polyketide synthase (ospks2) in male reproduction of rice (oryza sativa). recombinant ospks2 catalyzed the condensation of fatty acyl-coa with malonyl-coa to generate triketide and tetraketide α-pyrones, the main components of pollen exine. indeed, the ospks2 mutant had defec ...201728783252
foliar application of aspartic acid lowers cadmium uptake and cd-induced oxidative stress in rice under cd stress.cadmium (cd) contamination of farmland soils is a widespread problem around the globe, and rice (oryza sativa l.) tends to accumulate more cd and is considered as one of the major sources of cd intake in humans, especially consuming rice-derived products. the current study investigated the effects of foliar applied aspartic acid (asp) on growth parameters, biomass, chlorophyll concentration, gas exchange characteristics, cd uptake, and antioxidative capacity in the shoots and roots of rice seedl ...201728780693
heavy metals (lead, cadmium, methylmercury, arsenic) in commonly imported rice grains (oryza sativa) sold in saudi arabia and their potential health risk.the levels of heavy metals (lead, cadmium, methylmercury and arsenic) were determined in 37 brands of imported rice commonly consumed in saudi arabia after soaking and rinsing with water, and their potential health risks to residents were estimated by three indices: hazard quotient (hq), hazard index (hi) and cancer risk (cr). the mean levels of lead, cadmium, methylmercury and total arsenic in soaked (rinsed) rice grains were 0.034 (0.057), 0.015 (0.027), 0.004 (0.007) and 0.202 (0.183) μg/g dr ...201728780210
seed priming with se alleviate as induced phytotoxicity during germination and seedling growth by restricting as translocation in rice (oryza sativa l c.v. iet-4094).interactive aspect of among selenium (se) and as (as) to mitigate as induced phytotoxicity in rice during germination and seedling growth has been based on mostly to petriplates and hydroponic mode of experiments. in this investigation we explore the consequences of sowing se primed rice seeds in as spiked soil. unprimed, hydroprimed and se primed rice (iet-4094) seeds sown in as spiked soil, with five replications, arranged in complete randomized design for evaluating the impacts of seed primin ...201728779704
increment of root membrane permeability caused by microcystins result in more elements uptake in rice (oryza sativa).we conducted an indoor culture experiment to evaluate the phytotoxic effect of microcystins (mcs) on rice. after a 30day exposure, mcs induced a clear inhibition in rice growth, as well as a disruption of its antioxidant system and lipid peroxidation. we observed an increase in root membrane permeability; the conductivity of the leakage solution of the roots at 50 and 500μgl-1 was significantly increased by 77% and 136%, respectively, compared to the control. uptake of microelements (fe, mn, cu ...201728778041
multiple method analysis of tio2 nanoparticle uptake in rice (oryza sativa l.) plants.understanding the translocation of nanoparticles (nps) into plants is challenging because qualitative and quantitative methods are still being developed and the comparability of results among different methods is unclear. in this study, uptake of titanium dioxide nps and larger bulk particles (bps) in rice plant (oryza sativa l.) tissues was evaluated using three orthogonal techniques: electron microscopy, single-particle inductively coupled plasma mass spectroscopy (spicp-ms) with two different ...201728777911
simultaneous improvement and genetic dissection of salt tolerance of rice (oryza sativa l.) by designed qtl pyramiding.breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to enhance the rice productivity of abiotic stresses prone areas. it also poses the greatest challenge to plant breeders to breed rice varieties for such stress prone conditions. here, we carried out a designed qtl pyramiding experiment to develop high yielding "green super rice" varieties with significantly improved tolerance to salt stress and grain yield. using the f4 population derived from a cross b ...201728775730
thymol ameliorates cadmium-induced phytotoxicity in the root of rice (oryza sativa) seedling by decreasing endogenous nitric oxide generation.thymol has been developed as medicine and food preservative due to its immune-regulatory effect and antimicrobial activity, respectively. however, little is currently known about the role of thymol in the modulation of plant physiology. in the present study, we applied biochemical and histochemical approaches to investigate thymol-induced tolerance in rice (oryza sativa) seedlings against cd (cadmium) stress. thymol at 20 μm recovered root growth completely upon cdcl2 exposure. thymol pronounced ...201728771007
effect of selenium application on arsenic uptake in rice (oryza sativa l.).alluvial aquifers of the agrarian state of punjab of southwestern arid zone used for irrigation of rice crops are rich in arsenic concentration. in the present study, rice (oryza sativa l.) crops were raised in pots in a greenhouse with a purpose to study whether selenium (se) application was effective in ameliorating as uptake. the rice crop was irrigated with arsenic laced water (0, 2.5, 5.0, 10.0 μm as l-1) throughout the growing period, without and with selenium (0.05 and 0.10 mg kg-1) added ...201728770430
silicon supplementation alters the composition of herbivore induced plant volatiles and enhances attraction of parasitoids to infested rice plants.silicon (si) is important in plant defenses that operate in a direct manner against herbivores, and work in rice (oryza sativa) has established that this is mediated by the jasmonate signaling pathway. plant defenses also operate indirectly, by the production of herbivore induced plant volatiles (hipvs) that attract predators and parasitoids of herbivores. these indirect defenses too are mediated by the jasmonate pathway but no earlier work has demonstrated an effect of si on hipvs. in this stud ...201728769965
tightly controlled expression of bhlh142 is essential for timely tapetal programmed cell death and pollen development in rice.male sterility is important for hybrid seed production. pollen development is regulated by a complex network. we previously showed that knockout of bhlh142 in rice (oryza sativa) causes pollen sterility by interrupting tapetal programmed cell death (pcd) and bhlh142 coordinates with tdr to modulate the expression of eat1. in this study, we demonstrated that overexpression of bhlh142 (oe142) under the control of the ubiquitin promoter also leads to male sterility in rice by triggering the prematu ...201728769961
oryza sativa brassinosteroid upregulated1 like1 induces the expression of a gene encoding a small leucine-rich-repeat protein to positively regulate lamina inclination and grain size in rice.oryza sativa brassinosteroid upregulated1 like1 (osbul1) positively affects lamina inclination and grain size. osbul1 knock-out (osbul1) plants as well as transgenic rice with reduced level of osbul1 expression produce erect leaves and small grains. here, we identified a putative downstream gene of osbul1, osbul1 downstream gene1 (osbdg1) encoding a small protein with short leucine-rich-repeats by cdna microarray analyses in the lamina joint and panicles of wild-type and osbul1 plants. transgeni ...201728769958
elevated carbon dioxide alleviates aluminum toxicity by decreasing cell wall hemicellulose in rice (oryza sativa).carbon dioxide (co2) is involved in plant growth as well as plant responses to abiotic stresses; however, it remains unclear whether co2 is involved in the response of rice (oryza sativa) to aluminum (al) toxicity. in the current study, we discovered that elevated co2 (600 μl·l-1) significantly alleviated al-induced inhibition of root elongation that occurred in ambient co2 (400 μl·l-1). this protective effect was accompanied by a reduced al accumulation in root apex. al significantly induced ci ...201728769823
molecular characterization of rice arsenic-induced ring finger e3 ligase 2 (osair2) and its heterogeneous overexpression in arabidopsis thaliana.arsenic (as) accumulation adversely affects the growth and productivity of plants and poses a serious threat to human health and food security. in this study, we identified one as-responsive really interesting new gene (ring) e3 ubiquitin ligase gene from rice root tissues during as stress. we named it oryza sativa as-induced ring e3 ligase 2 (osair2). expression of osair2 was induced under various abiotic stress conditions, including heat, salt, drought and as exposure. results of an in vitro u ...201728766720
panicle blast 1 (pb1) resistance is dependent on at least four qtls in the rice genome.rice blast is the most serious disease afflicting rice and there is an urgent need for the use of disease resistance (r) genes in blast tolerance breeding programs. pb1 is classified as a quantitative resistance gene and it does not have fungal specificity. pb1-mediated resistance develops in the latter stages of growth. however, some cultivars, such as kanto209 (k209), cultivar name satojiman, despite possessing pb1, do not exert resistance to rice blast during the reproductive stage.201728766258
the effects of chilling stress after anthesis on the physicochemical properties of rice (oryza sativa l) starch.this study investigates the effect of chilling stress, over a period of three days after anthesis, on the physicochemical properties of starches derived from six rice cultivars. chilling stress significantly affected the grain characteristics and physicochemical properties of rice starches, except for those of two varieties, nj 9108 and zd 18. in the other four rice cultivars subjected to chilling stress, the content of medium, and large sized granules showed a decrease, and an increase, respect ...201728764089
effect of corrosion inhibitor benzotriazole on the uptake and translocation of cd in rice (oryza sativa l.) under different exposure conditions.emerging contaminants that can complex with heavy metals might affect the speciation of coexisting metals and result in different ecological risks. as a widely used metal corrosion inhibitor, 1h-benzotriazole (btr) is frequently detected in the environments, sometimes at very high levels. in this study, rice (oryza sativa l.) was used to assess the ecological risk of combined exposure to cadmium (cd) and btr in plants and discuss the potential effects of exposure sequence on the uptake and trans ...201728763635
optimized mass spectrometry-based metabolite extraction and analysis for the geographical discrimination of white rice (oryza sativa l.): a method comparison study.in this study, we examined the effects of different extraction methods for the gc-ms- and lc-ms-based metabolite profiling of white rice (oryza sativa l.). in addition, the metabolite divergence of white rice cultivated in either korea or china was also evaluated. the discrimination analysis of each extraction method for white rice from korea and china and the corresponding discriminatory markers were estimated by unpaired t-test, principal component analysis, k-means cluster analysis, partial l ...201728762322
effects and mechanisms of meta-sodium silicate amendments on lead uptake and accumulation by rice.the objectives of this research were to study the effects of na2sio3 application on the uptake, translocation, and accumulation of pb in rice and to investigate the mechanisms of pb immobilization by na2sio3 in paddy rice soils and rice plants. pot experiments were conducted using a cd-pb-zn-polluted soil and oryza sativa l. ssp. indica cv. donglian 5. l3-edge x-ray absorption spectroscopy was used to identify pb species in soils and roots. the results showed that the application of na2sio3 incr ...201728762046
connectivity in gene coexpression networks negatively correlates with rates of molecular evolution in flowering plants.gene coexpression networks are a useful tool for summarizing transcriptomic data and providing insight into patterns of gene regulation in a variety of species. though there has been considerable interest in studying the evolution of network topology across species, less attention has been paid to the relationship between network position and patterns of molecular evolution. here, we generated coexpression networks from publicly available expression data for seven flowering plant taxa (arabidops ...201728759647
assessment and genetic analysis of heavy metal content in rice grain using an oryza sativa × o. rufipogon backcross inbred line population.heavy metal accumulation in rice is a growing concern for public health. backcross inbred lines derived from an interspecific cross of oryza sativa × o. rufipogon were grown in two distinct ecological locations (hangzhou and lingshui, china). the objective of this study was to characterise the contents of heavy metal in rice grains, and to identify quantitative trait loci (qtls) for heavy metal contents.201728758676
linking hydrogen-enhanced rice aluminum tolerance with the reestablishment of ga/aba balance and mirna-modulated gene expression: a case study on germination.although previous results showed that exogenous hydrogen (h2) alleviated aluminum (al) toxicity, the detailed mechanism remains unclear. here, we reported that the exposure of germinating rice seeds to al triggered h2 production, followed by a decrease of ga/aba ratio and seed germination inhibition. compared to inert gas (argon), h2 pretreatment not only strengthened h2 production and alleviated al-induced germination inhibition, but also partially reestablished the balance between ga and aba. ...201728756251
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