Publications

variable methylation and differential replication of genomic dna in cultured carrot root expiants during growth induction as influenced by hormonal treatments.	the methylation and amplification pattern of genomic dna of carrot root expiants (daucus carota l.) undergoes transitory changes during the cultural cycle. a high degree of variation was observed as early as 36 h after the incubation of fresh expiants in the nutrient medium and, depending on the hormonal treatment significant modifications occurred during 14 days of culture. proliferative tissue conditioned by kinetin showed an extensive reduction in dna methylation. changes in the dna amplifica ...	1991	24213171
the impact of domestication on the genetic variability in the orange carrot, cultivated daucus carota ssp. sativus and the genetic homogeneity of various cultivars.	isozyme analysis of wild and domesticated accessions indicated that domestication of the cultivated carrot daucus carota ssp. sativus resulted in an insignificant reduction of all genetic variability and genetic distance estimates. although they are less variable genetically, cultivated forms maintain a high proportion of observed heterozygosity. relative to the overall genetic variability of the species, samples from four common cultivars 'red cored chantenay', 'scarlet nantes', 'danvers half l ...	1991	24213074
microtubule-binding proteins from carrot : i. initial characterization and microtubule bundling.	microtubules (mts) participate in several processes of fundamental importance to growth and development in higher plants, yet little is known about the proteins with which they associate. information about these molecules is important because they probably play a role in mediating functional and structural differences between various mt arrays. as a first step in gaining insight into this problem, we have isolated, from suspension-cultured cells of carrot (daucus carota l.), non-tubulin proteins ...	1989	24212347
modulation of auxin-binding proteins in cell suspensions : ii. isolation and initial characterization of carrot cell variants impaired in somatic embryogenesis.	cultured cell lines from carrot (daucus carota l.) with little or no embryogenic potential were examined for the auxin-binding capacity of their membranes. the lines belonged to different classes: (a) wild-type lines kept in culture for different periods (the longer the period, the lower being their embryogenic potential); (b) variants, isolated after mutagenesis, showing normal growth but a lack of embryogenic response; (c) auxin-resistant lines, isolated as colonies on solid media containing 4 ...	1992	24203204
the effect of growth retardants on anthocyanin production in carrot cell suspension cultures.	the effect of growth retardants on anthocyanin production was studied in wild carrot (daucus carota) cell suspension cultures. paclobutrazol [(2rs,3rs) - 1 - (4-chlorophenyl) - 4,4 -dimethyl-2-(1,2,4-triazol-1-yl) pentan-3-ol], uniconazole [(e)-1-(4-chlorophenyl-4,4 -) dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol], tetcyclacis [5-(4-chloro-phenyl) -3,4,5,9,10-pentaaza-tetracyclo-5, 4, 10(2,6), o(8,11) - dodeca-3, 9-diene], ancymidol [α-cyclopropyl - 4 - methoxy-α(pyrimidine-5-yl)benzyl alcohol] ...	1992	24203145
unique patterns of mitochondrial genes, transcripts and proteins in different male-sterile cytoplasms of daucus carota.	restriction fragment analysis of mitochondrial and chloroplast dnas from a brown anther and a petaloid cytoplasmic male-sterile (cms) line revealed unique patterns for each cms line distinct from those of normal fertile cytoplasms, but identical restriction fragments for all chloroplast dnas. the restauration of fertility through the introduction of nuclear restorer genes had no effect on the overall mitochondrial dna (mtdna) structure. the genomic environment and transcription patterns of sever ...	1992	24202587
patterns of expression of the jim4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in daucus carota l.	spatiotemporal patterns of expression of the cell-surface arabinogalactan-protein epitope defined by monoclonal antibody jim4 (j.p. knox et al., 1989, development 106, 47-56) have been characterized by indirect immunofluorescence during the process of somatic embryogenesis in daucus carota l. the jim 4 epitope (j4e) occurred on cells established in culture from hypocotyl explants which appeared to derive, at least in part, from the epidermal cells of the hypocotyl. cultures maintained in the pre ...	1990	24201958
identification of a wound-induced inhibitor of a nuclear factor that binds the carrot extensin gene.	following wounding of carrot (daucus carota l.) roots, the activity of a nuclear factor (egbf-1) that binds a 5'-region of the carrot extensin gene declines to undetectable levels within 48 h. mixing of nuclear extracts from wounded roots with nuclear extracts from unwounded roots has demonstrated the existence of a wound-induced inhibitor of egbf-1. inhibition of egbf-1 dna-binding activity by nuclear extracts from wounded roots is shown to be specific for egbf-1, and to be destroyed by heat tr ...	1989	24201846
induction of chalcone synthase in cell suspension cultures of carrot (daucus carota l. spp. sativus) by ultraviolet light: evidence for two different forms of chalcone synthase.	two cell lines of carrot (daucus carota l. spp. sativus), grown as cell-suspension cultures in the dark, were irradiated with ultraviolet light (315-420 nm) 10 d after the onset of cultivation. chalcone synthase (chs) enzyme activity was induced in both cell lines. anthocyanin synthesis was only stimulated in the anthocyanin-containing cell line dcb. parallel to the increase in chs activity there was an increase with time in the amount of one chs form with an isoelectric point of 6.5 and a molec ...	1989	24201661
structural analysis of the cell walls regenerated by carrot protoplasts.	a procedure was developed to isolate protoplasts rapidly from carrot (daucus carota l. cv. danvers) cells in liquid culture. high purity of cell-wall-degrading enzymes and ease of isolation each contributed to maintenance of viability and initiation of regeneration of the cell wall by a great majority of the protoplasts. we used this system to re-evaluate the chemical structure and physical properties of the incipient cell wall. contrary to other reports, callose, a (1 → 3)β-d-glucan whose synth ...	1989	24201658
site-specific binding of a nuclear factor to the carrot extensin gene is influenced by both ethylene and wounding.	experiments conducted in vitro using the electrophoretic mobility shift assay have shown that a single region of the extensin gene of carrot (daucus carota l.) interacts with a protein factor designated extensin gene binding factor-1 (egbf-1) present in nuclear extracts obtained from carrot roots. this interaction is sequence-specific as judged by the failure of other plant gene sequences to compete with the extensin gene for egbf-1 binding. the egbf-1 activity is organspecific, not being expres ...	1989	24201417
influence of a specific xyloglucan-nonasaccharide derived from cell walls of suspension-cultured cells of daucus carota l. on regenerating carrot protoplasts.	a xyloglucan oligosaccharide was isolated from cell walls of daucus carota l. suspension-cultured cells. from analytical data (gel-permeation chromatography, thin-layer chromatography, monosaccharide analysis, methylation analysis) it can be concluded that this oligosaccharide preparation consists mainly of a nonasaccharide known as xg9 (glc4xyl3galfuc). this nonasaccharide showed excellent "anti-auxin" properties in the pea-stem bioassay, with 80% inhibition of 2,4-dichlorophenoxyacetic acid (2 ...	1990	24197092
pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices.	monoclonal antibodies recognizing un-esterified (jim5) and methyl-esterified (jim7) epitopes of pectin have been used to locate these epitopes by indirect immunofluorescence and immunogold electron microscopy in the root apex of carrot (daucus carota l.). both antibodies labelled the walls of cells in all tissues of the developing root apex. immunogold labelling observed at the level of the electron microscope indicated differential location of the pectin epitopes within the cell walls. the un-e ...	1990	24196931
rapid changes in amplification and methylation pattern of genomic dna in cultured carrot root explants (daucus carota l.).	rapid genomic dna variation due to methylation and copy number alteration was observed in carrot root explants 6 h after inoculation and during a 36-h period of exponential callus growth. de novo methylation and amplification of restricted bspni fragments of low molecular weight occurred before cell cycle activation and should, therefore, be independent of progression through the s-phase of the cell cycle. growth regulators seemed to influence the amplification pattern indirectly by regulating c ...	1993	24196052
tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes.	somatic embryogenesis of carrot (daucus carota l.) is inhibited by the glycosylation inhibitor tunicamycin. this inhibition is reversible by the addition of correctly glycosylated glycoproteins which have been secreted into the culture medium. to identify the proteins responsible for complementation, glycoproteins present in the medium of embryo cultures were purified and tested for their activity in the tunicamycin inhibition/ complementation assay. a 38-kda glycoprotein was purified that could ...	1991	24194238
metabolic changes in carrot cells in response to simultaneous treatment with ultraviolet light and a fungal elicitor.	ultraviolet light induces anthocyanin biosynthesis in cell cultures of an afghan cultivar of daucus carota (daucus carota l. ssp. sativus). simultaneous treatment with a fungal elicitor from pythium aphanidermatum results in an inhibition of the catalytic activity of chalcone synthase (chs), which in turn correlates with an inhibition of anthocyanin biosynthesis. on immunoblots, one isoenzyme (40 kda) of chs disappears upon elicitor treatment. on an mrna level, only the mrna for the 40-kda-chs i ...	1991	24194154
gene expression during induction of somatic embryogenesis in carrot cell suspensions.	we report the identification, via their cdnas, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (daucus carota l.) cells cultured in an auxin-free medium. their expression is roughly associated with the first morphogenetic, or globular, stage. a cdna library (λ gt 10) was established using poly(a)(+) -rich rnas from cells deprived of auxin for 8 d. by differential screening a number of clones corresponding to early-induced embryogenic genes were ...	1991	24193528
acylation of anthocyanins with hydroxycinnamic acids via 1-o-acylglucosides by protein preparations from cell cultures of daucus carota l.	protein preparations from cell-suspension cultures of an afghan cultivar of daucus carota (carrot) catalyzed the formation of acylated anthocyanins from a cyanidin triglycoside isolated from the carrot cultures using (1-o-sinapoyl-, 1-o-feruloyl-) and 1-o-(p-coumaroyl)-β-glucose as acyl donors. the enzyme activities can be classified as 1-o-hydroxycinnamoyl-β-glucose: cyanidin 3-o-(2″-o-xylosyl-6″-o-glucosylgalactoside) 6‴-o-hydroxycinnamoyltransferases (ec 2.3.1.-).	1992	24186789
partial amino-acid sequence of ecp31, a carrot embryogenic-cell protein, and enhancement of its accumulation by abscisic acid in somatic embryos.	ecp31, an embryogenic-cell protein from carrot (daucus carota l.), was purified by sequential column-chromatographic steps and digested by v8 protease on a nitrocellulose membrane. the resultant peptides were separated by reverse-phased column chromatography and sequenced. the sequences obtained were 70-80% homologous to those of a late-embryogenesis-abundant protein (d34) from cotton (baker et al, 1988, plant mol. biol. 11, 227-291). the level of ecp31 in somatic embryos of carrot was increased ...	1992	24186729
initiation and proliferation of carrot callus using a combination of antibiotics.	calli were initiated from carrot (daucus carota l. subsp. sativus hoffm.) root expiants and grown on media supplemented with a combination of carbenicillin at 0.3 mg/ml and vancomycin at 0.1 mg/ml in the absence of plant hormones or hormone analogs. the growth rate was about half of that obtained with a combination of α-naphthaleneacetic acid and n(6)-benzyladenine at 1 mg/l each. carbenicillin in the combination with vancomycin could be replaced by penicillin g; other penicillins tested in this ...	1991	24186530
levels of p34(cdc2)-like protein in dividing, differentiating and dedifferentiating cells of carrot.	p34(cdc2) is a key cell-cycle protein in fission yeast that is necessary for progress in the cell cycle from the g1 to the s phase and from g2 through mitosis. homologues of p34(cdc2) have been found in all eukaryotes that have been investigated. levels of p34(cdc2)-like protein were studied by quantitative western blotting in developing cotyledons of daucus carota l. (carrot) seedlings, in expiants from the same seedlings transferred to tissueculture media with and without 2,4-dichlorophenoxyac ...	1991	24186410
immunohistochemical localization of a glycoprotein, gp80, in the outermost layer of the developing endosperm of immature seeds of carrot.	a glycoprotein (gp80) from the immature fruits of carrot (daucus carota l.) plants was purified through the sequential application of salt extraction, (nh4)2so4 precipitation, chromatography on columns of concanavalin a-sepharose, hydroxylapatite, and boronate, and electroelution, with subsequent sodium dodecylsulfate-polyacrylamide gel electrophoresis (sds-page). its apparent relative molecular mass (mr) was estimated to be 80 k by both sds-page and gel filtration at high ionic strength. the mr ...	1991	24186342
chlorsulfuron resistance in daucus carota cell lines and plants:involvement of gene amplification.	daucus carota l. cell lines stably resistant to the herbicide chlorsulfuron (cs) have been isolated according to a stepwise selection. studies carried out during different selection steps show that the specific activity of the target enzyme acetohydroxyacid synthase (ahas) increases along with cs resistance. southern hybridization analysis performed with abrassica napus ahas probe in a cs highly-resistant cell line reveals the presence of a greatly amplifiedecori fragment of genomic dna. this in ...	1994	24186104
production of somatic hybrids between daucus carota l. and nicotiana tabacum.	protoplasts of a kanamycin-resistant (kr, nuclear genome), streptomycin-resistant (sr, chloroplast genome) and chlorophyll-deficient (a1, nuclear genome) nicotiana tabacum (kr-sa) cell suspension cultures or x-ray-irradiated mesophyll protoplasts of kanamycin- and streptomycin-resistant green plants (kr-sr) were fused with protoplasts of a cytoplasmic male-sterile (cms) daucus carota l. cell suspension cultures by electrofusion. somatic hybrid plants were selected for kanamycin resistance and th ...	1994	24185885
sodium-hydrogen exchanger inhibitory potential of malus domestica, musa × paradisiaca, daucus carota, and symphytum officinale.	the involvement of sodium-hydrogen exchangers (nhe) has been described in the pathophysiology of diseases including ischemic heart and brain diseases, cardiomyopathy, congestive heart failure, epilepsy, dementia, and neuropathic pain. synthetic nhe inhibitors have not achieved much clinical success; therefore, plant-derived phytoconstituents may be explored as nhe inhibitors.	2014	24184990
isozyme patterns in zygotic and somatic embryogenesis of carrot.	isozyme patterns of carrot (daucus carota l.) zygotic embryos between the torpedo stage up to 5-day-old seedlings have been compared with those of the similar stages from the embryogenic cell suspension culture to the late somatic plantlet. somatic embryos blocked at the torpedo stage by β-cyclodextrine have also been analyzed. all these stages have been analyzed with respect to seven different enzyme systems: arylesterase, glucosephosphate isomerase, phosphogluconate dehydrogenase, alcohol dehy ...	1996	24178665
biosynthesis of p-hydroxybenzoic acid in elicitor-treated carrot cell cultures.	carrot (daucus carota l.) cells respond to treatment with fungal elicitors by synthesizing wallbound p-hydroxybenzoic acid (p-hba). the biosynthetic pathway to p-hba is still hypothetical. tracer experiments with l-phenylalanine indicate the involvement of the general phenylpropanoid pathway. 3,4 (methylenedioxy) innamic acid, an inhibitor of hydrocycinnamate coa ligase, inhibits the accumulation of anthocyanins in carrot, while it does not interfere with p-hba synthesis. thus p-hba biosynthesis ...	1992	24178394
cdna cloning of an extracellular dermal glycoprotein of carrot and its expression in response to wounding.	suspension-cultured cells of carrot (daucus carota l.) synthesize and secrete a glycoprotein that is normally found only in dermal tissues (epidermis, endodermis and periderm). this protein, previously called gp57, is now referred to as edgp (e xtracellular d ermal g lyco p rotein). we purified sufficient quantities of edgp to obtain amino-acid sequences on two internal tryptic peptides and screened a cdna library of young carrot roots with antiserum to edgp and with oligonucleotides correspondi ...	1992	24178334
dna methylation and dc8-gus transgene expression in carrot (daucus carota l.).	dna methylation has been associated with gene activity in differentiating and developing plant tissues. the objective of this study was to determine the involvement of methylation in the expression of a gene transferred into carrot (daucus carota l.) tissues by particle bombardment. expression of the dc8-gus gene construct in response to treatments with 5-azacytidine (s-azac) and to in vitro methylation by methylases was investigated by histochemical assay of gus activity. the 5-azac treatment i ...	1996	24178214
identification and localisation of a nucleoporin-like protein component of the plant nuclear matrix.	salt-detergent extraction of purified plant nuclei yields a fraction enriched in putative structural proteins known as the "nuclear matrix". compared with mammalian nuclear matrices, which contain three major proteins, plant nuclear matrices are complex, containing at least 100 polypeptides. in order to characterise more fully the plant nuclear matrix we have used antibodies raised against both yeast (saccharomyces cerevisiae) and mammalian (rat) nuclear pore proteins. we have shown that the nuc ...	1992	24178083
transgenic plants containing the phosphinothricin-n-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) differently from untransformed plants.	l-phosphinothricin (l-pt)-resistant plants were constructed by introducing a modified phosphinothricin-n-acetyl-transferase gene (pat) via agrobacterium-mediated gene transfer into tobacco (nicotiana tabacum l), and via direct gene transfer into carrot (daucus carota l). the metabolism of l-pt was studied in these transgenic, pt-resistant plants, as well as in the untransformed species. the degradation of l-pt, (14)c-labeled specifically at different c-atoms, was analysed by measuring the releas ...	1992	24177979
production and analysis of asymmetric hybrid plants between monocotyledon (oryza sativa l.) and dicotyledon (daucus carota l.).	asymmetric hybrid plants were obtained from fused protoplasts of a monocotyledon (oryza sativa l.) and a dicotyledon (daucus carota l.). x-ray-irradiated protoplasts isolated from a cytoplasmic malesterile (cms) carrot suspension culture were fused with iodoacetoamide-treated protoplasts isolated from a 5-methyltryptophan (5mt)-resistant rice suspension culture by electrofusion. the complementary recovered cells divided and formed colonies, which were then cultivated on regeneration medium suppl ...	1994	24177855
effect of pulsed electric field treatment on enzyme kinetics and thermostability of endogenous ascorbic acid oxidase in carrots (daucus carota cv. nantes).	the objective of this research was to study the enzyme kinetics and thermostability of endogenous ascorbic acid oxidase (aao) in carrot purée (daucus carota cv. nantes) after being treated with pulsed electric field (pef) processing. various pef treatments using electric field strength between 0.2 and 1.2kv/cm and pulsed electrical energy between 1 and 520kj/kg were conducted. the enzyme kinetics and the kinetics of aao thermal inactivation (55-70°c) were described using michaelis-menten model a ...	2014	24176379
physiological aspects of genome variability in tissue culture. ii. growth phase-dependent quantitative variability of repetitive bstni fragments of primary cultures of daucus carota l.	systematic investigations on the occurrence of differential dna replication in carrot cultures, expressed at the total genome level, were performed. the genome of daucus carota l. could be characterized by a pattern of repetitive bstni fragments that was independent of tissue specificity or cultivar differences. characterization of the genomic dna of the secondary phloem of carrot roots, in comparison to the dna of the induced primary cultures at different growth phases, revealed dramatic differ ...	1995	24169922
physiological aspects of genome variability in tissue culture. i. growth phase-dependent differential dna methylation of the carrot genome (daucus carota l.) during primary culture.	investigations were performed on growth phase-dependent ecorii site-specific dna methylation of the carrot genome during primary culture to elucidate physiological aspects of genome dna variability in tissue culture. while dna methylation of the root cambium and the secondary phloem and petioles of carrot leaves were strikingly different, the methylation level of the secondary phloem seemed to be independent of cultivar origin, the age of the plants and the extent of secondary root growth. as wa ...	1995	24169921
inheritance of chloroplast and mitochondrial dna in alloplasmic forms of the genus daucus.	the inheritance of mitochondrial (mt) and chloroplast (ct) dna in the progeny from interspecific crosses between the cultivated carrot (daucus carota sativus) and wild forms of the genus daucus was investigated by analysis of mt and ct rflps in single plants of the parental and filial generations. we observed a strict maternal inheritance of the organellar dnas in all interspecific crosses examined. previous studies on putative f2 plants from a cross between daucus muricatus x d. carota sativus ...	1995	24169891
mitochondrial dna diversity and male sterility in natural populations of daucus carota ssp carota.	mitochondrial variability was investigated in natural populations of wild carrot (daucus carota ssp carota) in different regions: south of france, greece, and various sites in the mediterranean basin and asia. total dna was digested with two restriction endonucleases (ecorv and hindiii) and probed with three mitochondrial dma-specific genes (coxi, atp6, and coxii). twenty-five different mitochondrial types were found in 80 analyzed individuals. thirteen mitotypes were found among the 7 french po ...	1995	24169681
a new protoplast culture system in daucus carota l. and its applications for mutant selection and transformation.	petiole protoplasts from in vitro-grown carrot plants are a very good alternative to traditionally obtained protoplasts from suspension cultures. high plating and regeneration efficiencies were obtained in most of the breeding lines that were tested. the embedding of the protoplasts in alginate was crucial for initiating cell division and further development. several streptomycin resistant and chlorophyll-deficient plant lines were selected for using the petiole protoplast system. maternally inh ...	1996	24162412
systemic allergic dermatitis caused by apiaceae root vegetables.	immediate hypersensitivity reactions to root vegetables of the umbelliferae plant family (apiaceae) is well known. delayed-type hypersensitivity is rarely reported.	2014	24102077
prediction model for cadmium transfer from soil to carrot (daucus carota l.) and its application to derive soil thresholds for food safety.	at present, soil quality standards used for agriculture do not fully consider the influence of soil properties on cadmium (cd) uptake by crops. this study aimed to develop prediction models for cd transfer from a wide range of chinese soils to carrot (daucus carota l.) using soil properties and the total or available soil cd content. path analysis showed soil ph and organic carbon (oc) content were the two most significant properties exhibiting direct effects on cd uptake factor (ratio of cd con ...	2013	24079518
dissimilarity of contemporary and historical gene flow in a wild carrot (daucus carota) metapopulation under contrasting levels of human disturbance: implications for risk assessment and management of transgene introgression.	transgene introgression from crops into wild relatives may increase the resistance of wild plants to herbicides, insects, etc. the chance of transgene introgression depends not only on the rate of hybridization and the establishment of hybrids in local wild populations, but also on the metapopulation dynamics of the wild relative. the aim of the study was to estimate gene flow in a metapopulation for assessing and managing the risks of transgene introgression.	2013	24052560
cooked carrot volatiles. aeda and odor activity comparisons. identification of linden ether as an important aroma component.	ms with gc-ri evidence was found for the presence of linden ether in cooked carrot (daucus carota). evaluation of the gc effluent from cooked carrot volatiles using aroma extract dilution analysis (aeda) found linden ether with the highest flavor dilution (fd) factor. others with 10-fold lower fd factors were β-ionone, eugenol, the previously unidentified β-damascenone, (e)-2-nonenal, octanal (+ myrcene), and heptanal. all other previously identified volatiles showed lower fd factors. odor thres ...	2013	24000828
theoretical and experimental models of hormetic fusion tubulogenesis.	hormetic morphogens are morphogens such as transforming growth factor beta (tgf-β) in mammals and auxin in plants that induce hormetic responses. for example, in vitro, tgf-β stimulates and inhibits cell proliferation at low and high concentrations respectively. i developed a model of hormetic morphogen gradient control of the morphogenesis of the fusion of bilateral aortic precursors (anlagen) that form the aorta during development; and validated the model with findings obtained by daucus carot ...	2013	23930100
biosynthesis of carotenoids in carrot: an underground story comes to light.	carrot (daucus carota) is a biannual plant that accumulates massive amounts of carotenoid pigments in the storage root. although the root of carrot plants was white before domestication, intensive breeding generated the currently known carotenoid-rich varieties, including the widely popular orange carrots that accumulate very high levels of the pro-vitamin a carotenoids β-carotene and, to a lower extent, α-carotene. recent studies have shown that the developmental program responsible for the acc ...	2013	23876238
exploring insights for virulent gene inhibition of multidrug resistant salmonella typhi, vibrio cholerae, and staphylococcus areus by potential phytoligands via in silico screening.	in our recent studies on prevalence of multidrug resistant pathogens in byramangala reservoir, karnataka, india, we identified salmonella typhi, staphylococcus aureus, and vibrio cholerae which had acquired multiple drug resistance (mdr) and emerged as superbugs. hence, there is a pressing demand to identify alternative therapeutic remedies. our study focused on the screening of herbal leads by structure-based virtual screening. the virulent gene products of these pathogens towards kanamycin(aph ...	2014	23876154
dcsto: carrot stowaway-like elements are abundant, diverse, and polymorphic.	we investigated nine families of stowaway-like miniature inverted-repeat transposable elements (mites) in the carrot genome, named dcsto1 to dcsto9. all of them were at-rich and shared a highly conserved 6 bp-long tir typical for stowaways. the copy number of dcsto1 elements was estimated as ca. 5,000 per diploid genome. we observed preference for clustered insertions of dcsto and other mites. distribution of dcsto1 hybridization signals revealed presence of dcsto1 clusters within euchromatic re ...	2013	23775534
quality of carrots as affected by pre- and postharvest factors and processing.	the aim of this review is to provide an update on factors contributing to quality of carrots, with special focus on the role of pre- and postharvest factors and processing. the genetic factor shows the highest impact on quality variables in carrots, causing a 7-11-fold difference between varieties in content of terpenes, β-carotene, magnesium, iron and phenolics as well as a 1-4-fold difference in falcarindiol, bitter taste and sweet taste. climate-related factors may cause a difference of up to ...	2013	23744724
indirect effects on mutualisms: parasitism of bumble bees and pollination service to plants.	researchers increasingly recognize the important role of mutualisms in structuring communities and view positive interactions in a community context rather than as simple pairwise interactions. indirect effects, such as those that predators have on lower trophic levels, are a key process in community ecology. however, such top-down indirect effects have rarely been extended to mutualisms. antagonists of one mutualist have the potential to negatively affect the second mutualist through negative e ...	2013	23691664
isolation and characterization of a carrot nucleolar protein with structural and sequence similarity to the vertebrate pescadillo protein.	the nuclear matrix is involved in many nuclear events, but its protein architecture in plants is still not fully understood. a cdna clone was isolated by immunoscreening with a monoclonal antibody raised against nuclear matrix proteins of daucus carota l. its deduced amino acid sequence showed about 40% identity with the pescadillo protein of zebrafish and humans. primary structure analysis of the protein revealed a pescadillo n-terminus domain, a single breast cancer c-terminal domain, two nucl ...	2013	23683933
influence of boron on carrot cell wall structure and its resistance to fracture.	plant cell wall structure integrity and associated tissue mechanical properties is one of key determinants for the perceived texture of plant-based foods. carrots (daucus carota) were used to investigate the effect of mineral supply of boron (b) and/or calcium (ca), during plant growth, on the plant cell wall structure and mechanical properties of matured root tissues. five commercial cultivars of carrots, kuroda (orange), dragon purple, kuttiger white, yellow, and nutri-red, were cultivated und ...	2010	23654242
comparison of protective and curative potential of daucus carota root extract on renal ischemia reperfusion injury in rats.	daucus carota linn (apiaceae), a useful vegetable, is traditionally used in treating kidney and hepatic dysfunctions.	2013	23627465
molecular characterisation of a calmodulin gene, vccam1, that is differentially expressed under aluminium stress in highbush blueberry.	calmodulin (cam), a small acidic protein, is one of the best characterised ca(2+) sensors in eukaryotes. this ca(2+) -regulated protein plays a critical role in decoding and transducing environmental stress signals by activating specific targets. many environmental stresses elicit changes in intracellular ca(2+) activity that could initiate adaptive responses under adverse conditions. we report the first molecular cloning and characterisation of a calmodulin gene, vccam1 (vaccinium corymbosum ca ...	2013	23627459
genetic structure and domestication of carrot (daucus carota subsp. sativus) (apiaceae).		2013	23594914
plant community characteristics and their responses to environmental factors in the water level fluctuation zone of the three gorges reservoir in china.	the annual water level regulating of the three gorges reservoir prolonged the submerged duration (from 2 to 8 months) and resulted in the reversal of natural flood rhythms (winter submerged). these changes might alter plant community characteristics in the water level fluctuation zone (wlfz). the aim of this study was to determine the plant community characteristics in the wlfz and their responses to the environmental factors (i.e., annual hydrological regulation, topographic characteristics, so ...	2013	23589274
an eco-metabolomic study of host plant resistance to western flower thrips in cultivated, biofortified and wild carrots.	domestication of plants and selection for agronomic traits may reduce plant secondary defence metabolites relative to their ancestors. carrot (daucus carota l.) is an economically important vegetable. recently, carrot was developed as a functional food with additional health-promoting functions. biofortified carrots contain increased concentrations of chlorogenic acid as an antioxidant. chlorogenic acid is involved in host plant resistance to western flower thrips (frankliniella occidentalis), o ...	2013	23583013
antibiotic uptake by plants from manure-amended soils.	antibiotics are extensively given to livestock to promote growth and reduce diseases. therefore, animal manure often contains antibiotics. once manure is applied to agricultural land to improve soil productivity, crops would be exposed to antibiotics which may persist in soils from a few to several hundred days. the objective of this study was to evaluate the uptake of gentamicin and streptomycin by carrot (daucus carota), lettuce (lactuca sativa) and radish (rhaphanus sativus) from manure-amend ...	2013	23581689
yield, chemical composition and nutritional quality responses of carrot, radish and turnip to elevated atmospheric carbon dioxide.	future concentration of carbon dioxide in the atmosphere is very important due to its apparent economic and environmental impact in terms of climate change. however, a compressive assessment of its effect on the nutritional and chemical characteristics of food crops has yet to be established. in the present study the impact of elevated atmospheric co2 on the yield, chemical composition and nutritional quality of three root vegetables, carrot (daucus carota l. cv. t-1-111), radish (raphanus sativ ...	2013	23576218
spontaneous spectinomycin resistance mutations detected after biolistic transformation of daucus carota l.	spectinomycin resistant mutant carrot (daucus carota l.) callus lines detected in the experiments on biolistic transformation of plastome were analyzed. it has been found that this antibiotic resistance is determined by point nucleotide substitutions at two distinct sites of the chloroplast gene rrn16, coding for 16s rrna, namely, g1012t, g1012c, and a1138g. the detected mutations are localized to the 16s rrna region forming helix h34, which contains spectinomycin binding site, and lead to its d ...	2011	23572997
effect of processing parameters on physico-chemical and culinary quality of dried carrot slices.	the investigation was carried out to evaluate the carrot (daucus carota) cultivars, to optimize the pre-treatments, time and temperature combination for drying carrot slices and to assess the suitability of the dried product for culinary preparations. among the 4 cultivars, ('pc-34', 'sel-21', 'ambala local' and 'nantes') the last one showed the best physico-chemical characteristics for dehydration. the dried carrot slices with highly desirable physico-chemical characteristics could be prepared ...	2011	23572730
in vivo antioxidant and hepatoprotective activity of methanolic extracts of daucus carota seeds in experimental animals.	to assess the in vivo antioxidfant and hepatoprotective activity of methanolic extract of daucus carota (d. carota) seeds in experimental animals.	2012	23569935
assessment of allelopathic properties of aloe ferox mill. on turnip, beetroot and carrot.	turnip (brassica rapa var. rapa l.), beetroot (beta vulgaris l.) and carrot (daucus carota l.) are common vegetables in south africa. the allelopathic potential of aqueous leaf and root extracts of aloe ferox mill.- a highly valued medicinal plant- was evaluated against seed germination and seedling growth of the three vegetables in petri dish experiments. the extracts were tested at concentrations of 2, 4, 6, 8, and 10 mg/ml. leaf extract concentrations above 4 mg/ml inhibited the germination o ...	2012	23558992
levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in daucus carota.	plant carotenoids are synthesized and accumulated in plastids through a highly regulated pathway. lycopene β-cyclase (lcyb) is a key enzyme involved directly in the synthesis of α-carotene and β-carotene through the cyclization of lycopene. carotenoids are produced in both carrot (daucus carota) leaves and reserve roots, and high amounts of α-carotene and β-carotene accumulate in the latter. in some plant models, the presence of different isoforms of carotenogenic genes is associated with an org ...	2013	23555569
identification of allergen-resolved threshold doses of carrot (daucus carota) by means of oral challenge and elisa.		2013	23498592
against the traffic: the first evidence for mitochondrial dna transfer into the plastid genome.	transfer of dna between different compartments of the plant cell, i.e., plastid, mitochondrion and nucleus, is a well-known phenomenon in plant evolution. six directions of inter-compartmental dna migration are possible in theory, however only four of them have been previously reported. these include frequent cases of mitochondrion and plastid to nucleus transfer, plastid to mitochondrion transfer, and rare nucleus to mitochondrion migrations. the connection between the plastid and mitochondrial ...	2012	23481035
effect of endogenous ascorbic acid oxidase activity and stability on vitamin c in carrots (daucus carota subsp. sativus) during thermal treatment.	the purpose of this research was to study the effect of endogenous ascorbic acid oxidase (aao) on vitamin c in carrots (daucus carota subsp. sativus), namely nantes, egmont gold and baby carrots during thermal treatment. enzyme-substrate reaction kinetics of aao were described using michaelis-menten equation. the estimated k(m) and v(max) values of aao ranged from 50.34 to 63.54 μm and 23.70 to 26.82 μmol/min, respectively. nantes carrots had the lowest aao activity. on the other hand, egmont go ...	2012	23442658
influence of field attack by carrot psyllid (trioza apicalis förster) on sensory quality, antioxidant capacity and content of terpenes, falcarindiol and 6-methoxymellein of carrots (daucus carota l.).	the effect of different degrees of attack by carrot psyllid (trioza apicalis) on quality parameters of carrots was studied in field experiments for two years. treatments were different degrees of physical insect protection by floating row cover. an increasing attack level of psyllids showed an enhancement effect on the antioxidant capacity (orac), content of falcarindiol, 6-methoxymellein, and terpenes, and scores for bitter taste, chemical flavor, terpene flavor, and toughness. carrot psyllid a ...	2013	23414489
wild vegetable mixes sold in the markets of dalmatia (southern croatia).	dalmatia is an interesting place to study the use of wild greens as it lies at the intersection of influence of slavs, who do not usually use many species of wild greens, and mediterranean culinary culture, where the use of multiple wild greens is common. the aim of the study was to document the mixtures of wild green vegetables which are sold in all the vegetable markets of dalmatia.	2013	23286393
reducing the bioavailability of pcbs in soil to plant by biochars assessed with triolein-embedded cellulose acetate membrane technique.	coupling with triolein-embedded cellulose acetate membrane (tecam) technique, hydroxypropyl β-cyclodextrins (hpcd) extraction method, and the greenhouse pot experiments, the influences of biochars on polychlorinated biphenyls (pcbs) bioavailability in soil to plant (brassica chinensis l. and daucus carota) were investigated. addition of 2% biochars to soils significantly reduced the uptake of pcbs in plant, especially for di-, tri- and tetra-chlorobiphenyls. pcbs concentrations in the roots of b ...	2013	23279904
influence of boron nutrition on net uptake and efflux of (32)p and (14)c-glucose in helianthus annuus roots and cell cultures of daucus carota.	(32)p and (14)c-glucose uptake were reduced under b deficiency in both daucus cell suspensions and helianthus roots. similarly, efflux rates were found to be smaller in b deficient material. efflux rates tended to be more affected than net uptake of both (32)p and glucose. this may explain why sunflower roots showed a higher glucose net uptake immediately after transferring from a b sufficient to a b deficient nutrient solution. the data confirm earlier findings of b as an essential element for ...	1985	23196176
selection and nuclear dna analysis of cell hybrids between daucus carota and oryza sativa.	in order to study the fate of the parental genomes in somatic cell hybrids between distantly related species, protoplasts from cultured cells of daucus carota and oryza sativa were fused. selective conditions resulted, exclusively, in the growth of hybrid colonies which combined the capacity to multiply of carrot cells with the natural resistance to a2ca of rice cells. a methodology for measuring the relative contribution of the parental cells to the hybrid nuclear genome has been worked out. th ...	1985	23196174
fusion of spinach mesophyll protoplasts with carrot root parenchyma protoplasts and the effect on spinach chloroplasts.	mesophyll protoplasts of spinach (spinacia oleracea l.) have been fused, using polyethylene glycol, with root parenchyma protoplasts of carrot (daucus carota l.) to enable studies of spinach chloroplasts in a foreign cytoplasm. after 24 h culture, electron microscopy of the fused protoplasts showed many intact chloroplasts, but some chloroplasts were completely disrupted, showing loss of chloroplast envelope and stroma. spinach protoplasts showed normal chloroplast structure 24 h after peg treat ...	1984	23196088
[cloning of eleutherococcus senticosus calmodulin gene and effect of endophytic fungus on expression amount of gene].	to clone calmodulin (cam) gene in eleutherococcus senticosus, and study the effect of endophytic fungi on expression amount of cam gene.	2012	23189731
morphological markers for the detection of introgression from cultivated into wild carrot (daucus carota l.) reveal dominant domestication traits.	hybridisation and subsequent introgression have recently received much attention in the context of genetically modified crops. but crop-wild hybrid detection in the field can be difficult, as most domestication traits seem to be recessive, and the hybrid phenotype may also depend on the direction of the cross or environmental factors. our aim was to develop a reliable set of morphological markers that differ between two wild and 13 cultivated carrots (daucus carota l.) and to evaluate their inhe ...	2013	23173917
carbonaceous soil amendments to biofortify crop plants with zinc.	carbonaceous soil amendments, comprising mixtures of biosolids and biochar, have been demonstrated to improve fertility while reducing nitrate leaching. we aimed to determine the efficacy of a biosolids/biochar soil amendment in biofortification of vegetables with zn, an element that is deficient in one third of humanity. we grew beetroot (beta vulgaris), spinach (spinacia oleracea), radish (raphanus sativus), broccoli (brassica oleracea), carrot (daucus carota), leek (allium ampeloprsum), onion ...	2013	23146312
clarification and pasteurisation effects on monomeric anthocyanins and percent polymeric colour of black carrot (daucus carota l.) juice.	black carrots (bcs) are a rich source of stable anthocyanins (acns). the purpose of this study was to evaluate the effects of clarification and pasteurisation on acns of black carrot juice (bcj). monomeric acns, acn profile and percent polymeric colour were determined during processing of bcj. while depectinisation and bentonite treatments resulted in 7% and 20% increases in monomeric acn content of bcj, respectively, gelatine-kieselsol treatment and pasteurisation resulted in 10% and 3-16% redu ...	2012	23107727
plants as biofactories: glyphosate-induced production of shikimic acid and phenolic antioxidants in wounded carrot tissue.	the use of plants to produce chemical compounds with pharmaceutical and nutraceutical applications has intensified in recent years. in this regard, genetic engineering is the most commonly used tool to generate crop lines with enhanced concentrations of desirable chemicals. however, growing genetically modified plants is still limited because they are perceived as potential biological hazards that can create an ecological imbalance. the application of postharvest abiotic stresses on plants induc ...	2012	23101679
molecular mapping of vernalization requirement and fertility restoration genes in carrot.	carrot (daucus carota l.) is a cool-season vegetable normally classified as a biennial species, requiring vernalization to induce flowering. nevertheless, some cultivars adapted to warmer climates require less vernalization and can be classified as annual. most modern carrot cultivars are hybrids which rely upon cytoplasmic male-sterility for commercial production. one major gene controlling floral initiation and several genes restoring male fertility have been reported but none have been mapped ...	2013	23015218
a bifasic response to cadmium stress in carrot: early acclimatory mechanisms give way to root collapse further to prolonged metal exposure.	very few studies have provided information about the effects of cadmium (cd) at histoanatomical and ultrastructural levels, along with potential localization of the metal in planta. in particular, from this standpoint, almost nothing is known in daucus carota l. (carrot), a particularly important species for in vitro and in vivo functional investigations. in this work we hypothesized that 36 μm cd, supplied for 1, 2, 3, 4, 7 and 14 days to 30-day-old in vitro-cultured plants, might induce an ear ...	2012	22868210
changes in carrot (daucus carota) parenchyma at higher temperatures detected in vivo by dynamic mechanical (thermal) analysis.	the outer parenchyma of carrot was tested using dynamic mechanical analysis (dma), in air with 90% humidity between 30 and 90°c. temperature plots of storage (sm i.e. elastic) and loss (lm i.e. inelastic) moduli were obtained. the sm and lm values were the basis for the calculation of the loss tangent (lt), the parameter expressing the ratio of inelastic to elastic parenchyma toughness. as expected, the tissue toughness decreased with increasing temperature. for both moduli, two characteristic t ...	2012	22836082
the antioxidant and anticancer effects of wild carrot oil extract.	daucus carota l. ssp. carota (apiacea) is used in traditional medicine in lebanon and in different regions throughout the world. the present study investigates the in vitro anticancer activities of daucus carota oil extract (dcoe) on four human cancer cell lines as well as its in vitro antioxidant activity. dcoe was extracted from the dried umbels with 50:50 acetone-methanol. the oil extract was analyzed by gas chromatography-mass spectrometry and screened for its antioxidant properties in vitro ...	2013	22815230
differential selection on carotenoid biosynthesis genes as a function of gene position in the metabolic pathway: a study on the carrot and dicots.	selection of genes involved in metabolic pathways could target them differently depending on the position of genes in the pathway and on their role in controlling metabolic fluxes. this hypothesis was tested in the carotenoid biosynthesis pathway using population genetics and phylogenetics.	2012	22737218
the potential of five winter-grown crops to reduce root-knot nematode damage and increase yield of tomato.	broccoli (brassica oleracea), carrot (daucus carota), marigold (tagetes patula), nematode-resistant tomato (solanum lycopersicum), and strawberry (fragaria ananassa) were grown for three years during the winter in a root-knot nematode (meloidogyne incognita) infested field in southern california. each year in the spring, the tops of all crops were shredded and incorporated in the soil. amendment with poultry litter was included as a sub-treatment. the soil was then covered with clear plastic for ...	2010	22736848
a protocol to remove colored metabolites and other inhibitors from plant tissues to facilitate rna isolation suitable for downstream applications.	this work developed a protocol to remove colored metabolites and other interfering substances to facilitate rna isolation. these metabolites otherwise hinder rna isolation and downstream applications. the developed protocol used sodium dodecyl sulphate, ethylenediamine tetra-acetic acid, and ethanol in a definite ratio that removed the said metabolites from the tissue and aided isolation of rna using the existing methods. the protocol was developed for red colored roots of arnebia euchroma and w ...	2012	22736512
predator-prey relationships on apiaceae at an organic farm.	orius insidiosus (say) and o. pumilio (champion) were confirmed to be sympatric in north central florida as the major predators of the florida flower thrips, frankliniella bispinosa (morgan), on flowers of queen anne's lace, daucus carota l. and false queen anne's lace, ammi majus l. f. bispinosa was the predominant thrips observed on both flowers but colonized d. carota to a greater extent and earlier in the season than a. majus. despite differences in the abundance of f. bispinosa on the two p ...	2012	22732606
nutritional content and antioxidant properties of pulp waste from daucus carota and beta vulgaris.	this study reports the chemical composition and antioxidant potential of pulp waste from two vegetables, carrot (daucus carota) and beetroot (beta vulgaris). different in vitro assays used for determining antioxidant potential of extracts of pulp wastes were: 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging capacity, reducing power and total antioxidant activity by phosphomolybdenum method. total polyphenols, tannins and antioxidative components such as vitamin c, total carotenoids and β- ...	2010	22691993
continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus gigaspora decipiens in root organ culture.	establishment of arbuscular mycorrhizal (am) germplasm collections is complex because of the obligate biotrophic nature of am fungi. only a few am species are routinely maintained in monoxenic culture with ri t-dna transformed roots as host. incorporation of new am species into this culture system is important for molecular, physiological, and taxonomical studies. here we report for the first time the successful monoxenic culture of gigaspora decipiens (ja2 strain) with transformed carrot (daucu ...	2012	22658317
molecular cloning, sequence characterization of a novel pepper gene nadp-icdh and its effect on cytoplasmic male sterility.	nadp-dependent isocitrate dehydrogenase (nadp-icdh) is an important enzyme involved in energy metabolism. the complete coding sequence of the pepper (capsicum annuum) nadp-icdh gene was amplified using a reverse transcriptase pcr based on the conserved sequence information of the tomato and other solanaceae plants and known highly homologous pepper ests. nucleotide sequence analysis revealed that the pepper nadp-icdh gene encodes a protein of 415 amino acids that has high homology with the prote ...	2012	22653649
high-throughput cryopreservation of plant cell cultures for functional genomics.	suspension-cultured cell lines from plant species are useful for genetic engineering. however, maintenance of these lines is laborious, involves routine subculturing and hampers wider use of transgenic lines, especially when many lines are required for a high-throughput functional genomics application. cryopreservation of these lines may reduce the need for subculturing. here, we established a simple protocol for cryopreservation of cell lines from five commonly used plant species, arabidopsis t ...	2012	22437846
content of carotenoids in roots of seventeen cultivars of daucus carota l.	the aim of this study was to compare the content of carotenoids in seventeen cultivars of carrots grown in poland. conventional orange cultivars with rarely grown were compared: white, yellow and purple with yellow core cultivars. to determine the content of carotenoids, extracts from lyophilized carrot roots were prepared and analyzed by spectrophotometric as well as hplc methods with dad detector. the highest content of carotenoids was found in cultivars: 'korund f(1)' (48 mg/100g of fresh wei ...	2012	22428130
[obtaining of hairy-root, callus and suspension carrot culture (daucus carota l.) able to accumulate human interferon alpha-2b].	here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of nantskaya and perfektzya variety with the highest level of recombinant human interferon alpha-2b accumulation exhibited the highest level of plant protein extract antiviral activity (up to 12.8 x 10(3) iu/mg tsp). the antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from parent organisms. however, the antiviral activity level of suspen ...	2016	22420216
fluorescent in situ hybridization with arbitrarily amplified dna fragments differentiates carrot (daucus carota l.) chromosomes.	carrot (daucus carota l.) chromosomes are small and poorly differentiated in size and morphology. here we demonstrate that fluorescent in situ hybridization (fish) signals derived from arbitrary pcr probes can be used for chromosome identification in carrot. to prepare probes, we searched for nonpolymorphic products abundantly amplified with arbitrary decamer primers in a group of accessions representing carrot genetic diversity. as a result, 13 fragments ranging in size from 517 to 1758 bp were ...	2012	22360760
mitochondrial gene diversity associated with the atp9 stop codon in natural populations of wild carrot (daucus carota ssp. carota).	mitochondrial genomes extracted from the wild populations of daucus carota have been used as a genetic resource by breeders of cultivated carrot, yet little is known concerning the extent of their diversity in nature. of special interest is an snp in the putative stop codon of the mitochondrial gene atp9 that has been associated previously with male-sterile and male-fertile phenotypic variants. in this study, either the sequence or pcr/rflp genotypes were obtained from the mitochondrial genes at ...	2012	22337997
alternative oxidase (aox) and phenolic metabolism in methyl jasmonate-treated hairy root cultures of daucus carota l.	methyl-jasmonate (mj)-treated hairy roots of daucus carota l. were used to study the influence of alternative oxidase (aox) in phenylpropanoid metabolism. phenolic acid accumulation, as well as total flavonoids and lignin content of the mj-treated hairy roots were decreased by treatment with salicylhydroxamic acid (sham), a known inhibitor of aox. the inhibitory effect of sham was concentration dependent. treatment with propyl gallate (pg), another inhibitor of aox, also had a similar inhibitory ...	2012	22326792
effects of bioactive compounds from carrots (daucus carota l.), polyacetylenes, beta-carotene and lutein on human lymphoid leukaemia cells.	new therapies for leukaemia are urgently needed. carrots have been suggested as a potential treatment for leukaemia in traditional medicine and have previously been studied in other contexts as potential sources of anticancer agents. indicating that carrots may contain bioactive compounds, which may show potential in leukaemia therapies. this study investigated the effects of five fractions from carrot juice extract (cje) on human lymphoid leukaemia cell lines, together with five purified bioact ...	2012	22263789
survival of three commercially available natural enemies exposed to michigan wildflowers.	flowering plants are often used in habitat management programs to conserve the arthropod natural enemies of insect pests. in this study, nine species of flowering plants representing six families commonly found in north america east of the rocky mountains were evaluated based on how much they extended the lifespans of three commercially available natural enemy species in cages with cut flower stems compared with cages containing water only. the natural enemies used in the experiments were a lady ...	2011	22251728
effects of genotype, soil type, year and fertilisation on sensory and morphological attributes of carrots (daucus carota l.).	the aim of the study was to investigate the effects of variety, soil type and fertilisation, and the interactions between these factors, on yield and quality traits of carrots.	2012	22231302
polyacetylenes in fresh and stored carrots (daucus carota): relations to root morphology and sugar content.	carrot roots contain polyacetylenes, reported to be both beneficial and distasteful when consumed by humans. this study aimed to investigate the relationships between polyacetylene contents, root morphology and sugar content in order to increase the opportunities to optimise the composition of polyacetylenes in carrots.	2012	22190221
the survival of e. coli o157:h7, s. typhimurium and l. monocytogenes in black carrot (daucus carota) juice.	in this study the survival and growth patterns of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in various concentrations of black carrot juice were investigated during incubation period at 4°c and 37°c for 7days. several parameters, such as juice concentration (%), ph, incubation temperature (°c) and time (days) were found effective on the survival of pathogens tested. although l. monocytogenes has been found to be the less resistant microorganism to the variable c ...	2011	22153385
identification of a dau c prplike protein (dau c 1.03) as a new allergenic isoform in carrots (cultivar rodelika).	up to 25% of food allergic subjects in central europe suffer from carrot allergy. until now, two isoforms of the major carrot (daucus carota) allergen dau c 1 have been described: dau c 1.01, comprising five variants (dau c 1.0101-dau c 1.0105) and dau c 1.02.	2012	22093066
effects of dietary fruits, vegetables and a herbal tea on the in vitro transport of cimetidine: comparing the caco-2 model with porcine jejunum tissue.	context: dietary botanicals are often consumed together with allopathic medicines, which may give rise to pharmacokinetic interactions. in vitro intestinal models are useful to identify botanical-drug interactions, but they may exhibit different expressions of transporters or enzymes. objective: to compare the effects of selected dietary botanical extracts on cimetidine transport across two in vitro intestinal models. materials and methods: bi-directional transport of cimetidine was measured ...	2011	22085278