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dust mite infestation in cooking flour: experimental observations and practical recommendations.the first documented case of oral mite anaphylaxis has recently been reported in thailand, with mites possibly originating from cooking flour.201526141033
sensory and physicochemical evaluation of low-fat chicken mortadella with added native and modified starches.the objective of this work was to evaluate the effect of adding different starches (native and modified) on the physicochemical, sensory, structural and microbiological characteristics of low-fat chicken mortadella. two formulations containing native cassava and regular corn starch, coded cass (5.0 % of cassava starch) and corn (5.0 % of regular corn starch), and one formulation produced with physically treated starch coded as mod1 (2.5 % of novation 2300) and chemically modified starch coded as ...201526139901
two-step production of gamma-aminobutyric acid from cassava powder using corynebacterium glutamicum and lactobacillus plantarum.production of gamma-aminobutyric acid (gaba) from crop biomass such as cassava in high concentration is desirable, but difficult to achieve. a safe biotechnological route was investigated to produce gaba from cassava powder by c. glutamicum g01 and l. plantarum gb01-21. liquefied cassava powder was first transformed to glutamic acid by simultaneous saccharification and fermentation with c. glutamicum g01, followed by biotransformation of glutamic acid to gaba with resting cells of l. plantarum g ...201526115763
factors influencing somatic embryogenesis, regeneration, and agrobacterium-mediated transformation of cassava (manihot esculenta crantz) cultivar tme14.routine production of large numbers of transgenic plants is required to fully exploit advances in cassava biotechnology and support development of improved germplasm for deployment to farmers. this article describes an improved, high-efficiency transformation protocol for recalcitrant cassava cultivar tme14 preferred in africa. factors that favor production of friable embryogenic calli (fec) were found to be use of dkw medium, crushing of organized embryogenic structures (oes) through 1-2 mm siz ...201526113851
industrial production of 2,3-butanediol from the engineered corynebacterium glutamicum.the platform chemical 2,3-butanediol (2,3-bdo) is a valuable product that can be converted into several petroleum-based chemicals via simple chemical reactions. here, we produced 2,3-bdo with the non-pathogenic and rapidly growing corynebacterium glutamicum. to enhance the 2,3-bdo production capacity of c. glutamicum, we introduced buda encoding acetolactate decarboxylase from klebsiella pneumoniae, a powerful 2,3-bdo producer. additionally, budb (encoding α-acetolactate synthase) and budc (enco ...201526113219
biocatalytic synthesis of starch esters by immobilized lipase on magnetic microparticles.enzymatic esterification is an efficient approach for modifying starch to functionalized biomaterials. in this study, conversion of cassava starch to fatty acid acyl esters using immobilized thermomyces lanuginosus lipase on fe3o4 microparticles modified with 3-aminopropyltriethoxysilane and covalently linked by 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and n-hydroxysuccinimide (fe3o4-ap-ed-lipase) in a solvent-free system was studied. an optimized reaction containing 5% w/v gelatinized st ...201526107973
intake of partially defatted brazil nut flour reduces serum cholesterol in hypercholesterolemic patients--a randomized controlled trial.thyroid hormones can lower levels of atherogenic lipoproteins, and selenium is important in thyroid hormone homeostasis. we aimed to investigate the effects of a healthy diet associated with the brazil nut (bertholletia excelsa) in dyslipidemic and hypertensive patients.201526077768
effect of steaming process on new formulation and physical properties of earthworm-based fish pellets for african catfish (clarias gariepinus).fish feed has been recognized as one of main part/unit in aquaculture industry. however, current fish feed faces few challenges in terms of health aspects and cost issues. alternatively, new nutritional and economical/low cost formulation of fish pellets was designed by combination of earthworm powder and other economical ingredients such as fishmeal, soybean waste, rice bran and tapioca flour. the formulation was calculated using pearson's square and optimized by one-factor-at-time (ofat) metho ...201426031027
low cost medium for spore production of bacillus kku02 and kku03 and the effects of the produced spores on growth of giant freshwater prawn (macrobrachium rosenbergii de man).in order to extend the shelf life of 2 high potential bacillus probiotic isolates which were bacillus kku02 and bacillus kku03, the spore forms of these 2 bacillus isolates were studied for using as probiotic instead. the low cost medium for spore production of these 2 bacillus isolates was examined in order to produce probiotic spores for feeding the shrimps. it was found that cassava at 100 g l(-1) and supplemented with 20.0 g l(-1) dextrose, 0.1 g l(-1) mgso4 and 2.0 g l(-1) (nh4)2so4 showed ...201426031020
cassava brown streak disease: a threat to food security in africa.cassava brown streak disease (cbsd) has emerged as the most important viral disease of cassava (manihot esculenta) in africa and is a major threat to food security. cbsd is caused by two distinct species of ipomoviruses, cassava brown streak virus and ugandan cassava brown streak virus, belonging to the family potyviridae. previously, cbsd was reported only from the coastal lowlands of east africa, but recently it has begun to spread as an epidemic throughout the great lakes region of east and c ...201526015320
the agrobacterium tumefaciens ti plasmid virulence gene vire2 reduces sri lankan cassava mosaic virus infection in transgenic nicotiana benthamiana plants.cassava mosaic disease is a major constraint to cassava cultivation worldwide. in india, the disease is caused by indian cassava mosaic virus (icmv) and sri lankan cassava mosaic virus (slcmv). the agrobacterium ti plasmid virulence gene vire2, encoding a nuclear-localized, single-stranded dna binding protein, was introduced into nicotiana benthamiana to develop tolerance against slcmv. leaf discs of transgenic n. benthamiana plants, harboring the vire2 gene, complemented a vire2 mutation in a. ...201526008704
heterologous expression of melea3: a 10 kda late embryogenesis abundant protein of cassava, confers tolerance to abiotic stress in escherichia coli with recombinant protein showing in vitro chaperone activity.late embryogenesis abundant (lea) proteins are small molecular weight proteins involved in acquisition of tolerance to drought, salinity, high temperature, cold, and freezing stress in many plants. previous studies revealed a cdna sequence coding for a 10 kda atypical lea protein, named melea3, predicted to be located into mitochondria with potential role in salt stress response of cassava (manihot esculenta crantz). here we aimed to produce the recombinant melea3 protein by heterologous express ...201525990084
phylogeny and expression pattern of starch branching enzyme family genes in cassava (manihot esculenta crantz) under diverse environments.starch branching enzyme (sbe) is one of the key enzymes involved in starch biosynthetic metabolism. in this study, six sbe family genes were identified from the cassava genome. phylogenetic analysis divided the mesbe family genes into dicot family a, b, c, and the new group. tissue-specific analysis showed that mesbe2.2 was strongly expressed in leaves, stems cortex, and root stele, and mesbe3 had high expression levels in stem cortex and root stele of plants in the rapid growth stage under fiel ...201525981533
increasing stringiness of low-fat mozzarella string cheese using polysaccharides.when fat content of pasta filata cheese is lowered, a loss of fibrous texture occurs and low-fat (lf) mozzarella cheese loses stringiness, making it unsuitable for the manufacture of string cheese. we investigated the use of various polysaccharides that could act as fat mimetics during the stretching and extruding process to aid in protein strand formation and increase stringiness. low-fat mozzarella cheese curd was made, salted, and then 3.6-kg batches were heated in hot (80°) 5% brine, stretch ...201525981060
cytokinin-dependent secondary growth determines root biomass in radish (raphanus sativus l.).the root serves as an essential organ in plant growth by taking up nutrients and water from the soil and supporting the rest of the plant body. some plant species utilize roots as storage organs. sweet potatoes (ipomoea batatas), cassava (manihot esculenta), and radish (raphanus sativus), for example, are important root crops. however, how their root growth is regulated remains unknown. in this study, we characterized the relationship between cambium and radial root growth in radish. through a c ...201525979997
identification and distribution of the nbs-lrr gene family in the cassava genome.plant resistance genes (r genes) exist in large families and usually contain both a nucleotide-binding site domain and a leucine-rich repeat domain, denoted nbs-lrr. the genome sequence of cassava (manihot esculenta) is a valuable resource for analysing the genomic organization of resistance genes in this crop.201525948536
yield and properties of ethanol biofuel produced from different whole cassava flours.the yield and properties of ethanol biofuel produced from five different whole cassava flours were investigated. ethanol was produced from five different whole cassava flours. the effect of quantity of yeast on ethanol yield, effect of whole cassava flour to acid and mineralized media ratio on the yield of ethanol produced, and the physical properties of ethanol produced from different cassava were investigated. physical properties such as distillation range, density, viscosity, and flash point ...201325937983
prevalence and genetic diversity of endosymbiotic bacteria infecting cassava whiteflies in africa.cassava provides over half of the dietary requirement for more than 200 million poor in africa. in recent years, cassava has been affected by an epidemic of a virus disease called cassava brown streak disease (cbsd) that is spreading in much of eastern and central africa, affecting food security and the economic development of the poor. the viruses that cause cbsd are transmitted by the insect vector whitefly (bemisia tabaci), which have increased to very high numbers in some african countries. ...201525933928
sequences enhancing cassava mosaic disease symptoms occur in the cassava genome and are associated with south african cassava mosaic virus infection.cassava is an important food security crop in sub-saharan africa. two episomal begomovirus-associated sequences, named sequences enhancing geminivirus symptoms (segs1 and segs2), were identified in field cassava affected by the devastating cassava mosaic disease (cmd). the sequences reportedly exacerbated cmd symptoms in the tolerant cassava landrace tme3, and the model plants arabidopsis thaliana and nicotiana benthamiana, when biolistically co-inoculated with african cassava mosaic virus-camer ...201625920485
resistance to sri lankan cassava mosaic virus (slcmv) in genetically engineered cassava cv. ku50 through rna silencing.cassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. cassava cultivar ku50, the most widely grown cultivar for non-food purposes is susceptible to sri lankan cassava mosaic virus (slcmv). the objective of this work was to engineer resistance to slcmv by rna interference (rnai) in order to increase biomass yield, an important aspect for bioethanol production. here, we produced transgenic ku50 lines expressing dsrna homol ...201525901740
efficient reduction of the formation of by-products and improvement of production yield of 2,3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered klebsiella oxytoca in mineral salts medium.klebsiella oxytoca kms005 (∆adhe∆acka-pta∆ldha) was metabolically engineered to improve 2,3-butanediol (bdo) yield. elimination of alcohol dehydrogenase e (adhe), acetate kinase a-phosphotransacetylase (acka-pta), and lactate dehydrogenase a (ldha) enzymes allowed bdo production as a primary pathway for nadh re-oxidation, and significantly reduced by-products. kms005 was screened for the efficient glucose utilization by metabolic evolution. kms005-73t improved bdo production at a concentration o ...201525895450
physicochemical properties and responses in microcirculation of native tapioca starch-based plasma expander.plasma expanders (pes) such as hydroxyethyl strach are widely used for volume replacement. the plantation and production of tapioca in thailand is abundant which may provide a new source for pes starch with novel properties. this work investigated the properties and circulatory effects of native tapioca starch-based pe (tpe). various formulations of mixture between native tapioca starch and 0.9% sodium chloride solution were prepared and characterized in order to obtain the proper physicochemica ...201625892486
a genetic map of cassava (manihot esculenta crantz) with integrated physical mapping of immunity-related genes.cassava, manihot esculenta crantz, is one of the most important crops world-wide representing the staple security for more than one billion of people. the development of dense genetic and physical maps, as the basis for implementing genetic and molecular approaches to accelerate the rate of genetic gains in breeding program represents a significant challenge. a reference genome sequence for cassava has been made recently available and community efforts are underway for improving its quality. cas ...201525887443
antidiarrheal activity of ethanolic extract of manihot esculenta crantz leaves in wistar rats.use of manihot esculenta crantz (mec) plant has been mentioned in literature of food and agriculture organization of united nations, central tuber crops research institute and many others. it is also known commonly as tapioca, continues to be a crop of food security for the millions of people, especially in the developing countries of the globe including india. medicinal uses of this plant including diarrhea have been mentioned in literature, but scientific evidence is lacking.201725878462
use of dried waste of cassava starch extraction for feeding lactating cows.the aim of this study was to determine the best level of utilization of dried waste of cassava starch extraction (wcsed) as a substitute for corn for lactating cows. four lactating cows were fed diets with increasing levels (0%, 33%, 66% and 100%) of wcsed as a substitute for corn. the intake and digestibility of dry matter and nutrients, milk production and composition, blood parameters of glucose and urea and microbial synthesis of the diets were evaluated. there was a reduction in dry matter ...201525860971
detection of plant-based adulterants in turmeric powder using dna barcoding.in its powdered form, turmeric [curcuma longa l. (zingiberaceae)], a spice of medical importance, is often adulterated lowering its quality.201525853978
microbiological and chemical characteristics of tarubá, an indigenous beverage produced from solid cassava fermentation.the aim of this work was to identify and characterize the microbiota present during fermentation and in the final beverage, tarubá, by culture-dependent and -independent methods. in addition, target chemical compounds (carbohydrates, organic acids, and ethanol) were evaluated. lactic acid bacteria (lab) and mesophilic bacteria were the predominant microorganisms. among them, lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides, and bacillus subtilis were frequently isolated a ...201525846929
comparative study of the methane production based on the chemical compositions of mangifera indica and manihot utilissima leaves.leaves of mangifera indica (mi, mango leaves) and manihot utilissima (mu, cassava leaves) are available in tropical regions and are the most accessible vegetal wastes of kinshasa, capital of democratic republic of congo. these wastes are not suitably managed and are not rationally valorized. they are abandoned in full air, on the soil and in the rivers. they thus pollute environment. by contrast, they can be recuperated and treated in order to produce methane (energy source), organic fertilizer ...201525825684
[chemical characterization and quantification of fructooligosaccharides, phenolic compounds and antiradical activity of andean roots and tubers grown in northwest of argentina].there is great interest in consuming foods that can provide the nutrients for a good nutrition and other health beneficial compounds. the aim of this work was to determine the chemical composition of native foods of the andean region and to quantify some functional com-ponents. proximal composition, vitamin c, total phenolic compounds, antiradical activity (dpph) in peel and pulp, dietary fiber soluble and insoluble, fructooligosaccharides (fos), total and resistant starch (in tubers and raw roo ...201425799690
phylogenetic analysis and biolistic infectivity of a cloned sri lankan cassava mosaic virus dna-a from tamil nadu, india on nicotiana benthamiana.the complete nucleotide sequence of sri lankan cassava mosaic virus (slcmv) dna-a isolated from cassava in southern india was analyzed, a phylogenetic analysis with other begomoviral nucleotide sequences was performed and an efficient inoculation method of nicotiana benthamiana with the cloned dna was developed utilizing a biolistic device. the nucleotide sequence showed the conservation of all functional begomoviral protein domains and aligned most closely with begomoviruses reported from the i ...201525790052
microbial population, chemical composition and silage fermentation of cassava residues.in order to effectively use the cassava (manihot esculenta crantz) residues, including cassava leaves, peel and pulp for livestock diets, the chemical and microbiological composition, silage preparation and the effects of lactic acid bacteria (lab) inoculants on silage fermentation of cassava residues were studied. these residues contained 10(4) to 10(5) lab and yeasts, 10(3) to 10(4) coliform bacteria and 10(4) aerobic bacteria in colony forming units (cfu) on a fresh matter (fm) basis. the mol ...201525781881
high-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity-reducing process.viscosity reduction has a great impact on the efficiency of ethanol production when using roots and tubers as feedstock. plant cell wall-degrading enzymes have been successfully applied to overcome the challenges posed by high viscosity. however, the changes in cell wall polymers during the viscosity-reducing process are poorly characterized. comprehensive microarray polymer profiling, which is a high-throughput microarray, was used for the first time to map changes in the cell wall polymers of ...201725757626
classification of cassava genotypes based on qualitative and quantitative data.we evaluated the genetic variation of cassava accessions based on qualitative (binomial and multicategorical) and quantitative traits (continuous). we characterized 95 accessions obtained from the cassava germplasm bank of embrapa mandioca e fruticultura; we evaluated these accessions for 13 continuous, 10 binary, and 25 multicategorical traits. first, we analyzed the accessions based only on quantitative traits; next, we conducted joint analysis (qualitative and quantitative traits) based on th ...201525730029
studies on differential behavior of cassava mosaic geminivirus dna components, symptom recovery patterns, and their sirna profiles.cassava mosaic disease caused by cassava mosaic geminiviruses (cmgs) with bipartite genome organization is a major constraint for production of cassava in the african continent and the indian sub-continent. currently, there are eleven recognized species of cmgs, and several diverse isolates represent them, with vast amount of sequence variability, reflecting into diversity of symptom severity/phenotypes. here, we make a systematic effort to study the infection dynamics of several species of cmgs ...201525724177
molecular characterization of a new begomovirus associated with leaf yellow mosaic disease of jatropha curcas in india.during a survey in june 2011, severe leaf yellow mosaic disease was observed on about 45 % plants of jatropha curcas growing in the katerniaghat wildlife sanctuary in india. an association of a begomovirus with disease was detected in 15 out of 20 samples by pcr using begomovirus genus-specific primers and total dna isolated from symptomatic leaf samples. for identification of the begomovirus, the complete genome was amplified using a phi-29 dna-polymerase-based rolling-circle amplification kit ...201525716923
characteristics of wet and dried distillers grains on in vitro ruminal fermentation and effects of dietary wet distillers grains on performance of hanwoo steers.two experiments were conducted to evaluate the nutrient composition, in vitro dry matter disappearance (ivdmd) and organic matter disappearance (ivomd) of three kinds of distillers grains (dg); i) wet distillers grains (wdg, krw 25/kg), ii) dried distillers grains (ddg, krw 280/kg), iii) dried distillers grains with solubles (ddgs, krw 270/kg) produced from tapioca 70% and rice 30%, and to evaluate dietary effects of wdg on the performance of hanwoo steers. in exp. 1, twelve-wdg, four-ddg and on ...201525715686
an overview of protein identification studies in cassava.cassava (manihot esculenta crantz) belongs to the euphorbiaceae family and is originated from the southern amazon basin. the storage root is the most important product of cassava as food for more than 800 million people in africa, asia and latin america. in this review, we present a retrospective of studies aiming the identification of cassava proteins, starting from the first investigations using sds-page and classical two-dimensional gel electrophoresis (2de) to recent studies with advanced te ...201525707473
correlation of chemical compositions of cassava varieties to their resistance to prostephanus truncatus horn (coleoptera: bostrichidae).the preference of cassava as a major host by prostephanus truncatus horn is a major constraint to ample production of cassava, manihot esculenta crantz and storage. this study analyzed the nutritional and secondary metabolite compositions in 15 cassava varieties, evaluated levels of damage and reproduction by p. truncatus, and assessed their resistance to attack. one hundred grams of dried cassava chips in 250-ml kilner jars were infested with 10 adult larger grain borerof 0-10 days old and held ...201525700536
cassava peel as a replacement for corn in the diet of lactating cows.the aim of this study was to evaluate the effect of the replacement of corn with cassava peel in the diets of dairy cows. eight holstein cows were used. the experimental treatments consisted of four replacement levels of corn with cassava peel (0, 33, 66, and 100 %). the replacement of corn with cassava peel linearly decreased the intake and digestibility of dry matter and organic matter, but did not alter the intake of neutral detergent fiber and crude protein. in addition, there was a linear d ...201525686553
optimisation of a cheap and residential small-scale production of edible crickets with local by-products as an alternative protein-rich human food source in ratanakiri province, cambodia.the health status of the indigenous people of ratanakiri province, cambodia, is significantly lower compared with that of the rest of the nation. the domestication and mass production of insects may represent a sustainable, cost effective and high quality alternative source of protein to traditional livestock. this study aimed to optimise a cheap and residential cricket breeding system based on unused wild resources. the development of crickets, teleogryllus testaceus (walker), under seven diets ...201625683556
starch (tapioca) to carbon dots: an efficient green approach to an on-off-on photoluminescence probe for fluoride ion sensing.photoluminescent carbon dots of 4-5 nm size were prepared from starch (tapioca sago) through a solution method under mild conditions. the as-prepared carbon dots were used as photoluminescence probes for highly anion selective fluoride ion detection in aqueous solutions. a ready-to-use device is also demonstrated.201525679995
effects of a food-based intervention on markers of micronutrient status among indian women of low socio-economic status.intakes of micronutrient-rich foods are low among indian women of reproductive age. we investigated whether consumption of a food-based micronutrient-rich snack increased markers of blood micronutrient concentrations when compared with a control snack. non-pregnant women (n 222) aged 14-35 years living in a mumbai slum were randomised to receive a treatment snack (containing green leafy vegetables, dried fruit and whole milk powder), or a control snack containing foods of low micronutrient conte ...201525677713
endophytic fungi from the amazonian plant paullinia cupana and from olea europaea isolated using cassava as an alternative starch media source.endophytic fungi live inside plants, apparently do not cause any harm to their hosts and may play important roles in defense and growth promotion. fungal growth is a routine practice at microbiological laboratories, and the potato dextrose agar (pda) is the most frequently used medium because it is a rich source of starch. however, the production of potatoes in some regions of the world can be costly. aiming the development of a new medium source to tropical countries, in the present study, we u ...201325674409
chemical constituents from the stems of manihot esculenta.two new compounds, maniesculentins a (1) and b (6), together with four known ones were isolated from the stems of manihot esculenta crantz. the structures of the new compounds were elucidated by extensive spectroscopic methods including nmr spectroscopy and mass spectrometry. the two new compounds (1, 6) were assayed for antibacterial activity against four tested bacteria lines.201525673419
rheological, physical, and sensory attributes of gluten-free rice cakes containing resistant starch.in this study the effect of resistant starch (rs) addition on gluten-free cakes from rice flour and tapioca starch physical and sensorial properties was investigated. increase in rs concentration made cake batters less elastic (drop of g'(ω), g''(ω) values) and thinner (viscosity decreased). cakes specific volume increased with an increase in rs level and was maximized for 15 g/100 g rs, although porosity values were significantly unaffected by rs content. crumb grain analysis exhibited a decrea ...201525604540
cassava virus diseases: biology, epidemiology, and management.cassava (manihot esculenta crantz.) is the most important vegetatively propagated food staple in africa and a prominent industrial crop in latin america and asia. its vegetative propagation through stem cuttings has many advantages, but deleteriously it means that pathogens are passed from one generation to the next and can easily accumulate, threatening cassava production. cassava-growing continents are characterized by specific suites of viruses that affect cassava and pose particular threats. ...201525591878
control of sweet potato virus diseases.sweet potato (ipomoea batatas) is ranked seventh in global food crop production and is the third most important root crop after potato and cassava. sweet potatoes are vegetative propagated from vines, root slips (sprouts), or tubers. therefore, virus diseases can be a major constrain, reducing yields markedly, often more than 50%. the main viruses worldwide are sweet potato feathery mottle virus (spfmv) and sweet potato chlorotic stunt virus (spcsv). effects on yields by spfmv or spcsv alone are ...201525591876
the dynamics and environmental influence on interactions between cassava brown streak disease and the whitefly, bemisia tabaci.cassava brown streak disease (cbsd) is currently the most significant virus disease phenomenon affecting african agriculture. in this study, we report results from the most extensive set of field data so far presented for cbsd in africa. from assessments of 515 farmers' plantings of cassava, incidence in the coastal zone of tanzania (46.5% of plants; 87% of fields affected) was higher than in the lake zone (22%; 34%), but incidences for both zones were greater than previous published records. th ...201525585059
assessing the effect of composting cassava peel based substrates on the yield, nutritional quality, and physical characteristics of pleurotus ostreatus (jacq. ex fr.) kummer.cassava peel based substrate formulations as an alternative substrate were used to grow mushrooms. the effect of two compost heights, three composting periods on the mycelia growth, physical characteristics, yield, and nutritional qualities of pleurotus ostreatus (jacq. ex fr.) kummer was studied. mean mycelia growth of 16.2 cm after a period of seven (7) weeks was the best for 1.5 m compost height. cap diameter and stipe length differed significantly (p < 0.05) with the compost heights (0.8 m a ...201425580299
potential of tannin-rich plants for modulating ruminal microbes and ruminal fermentation in sheep.the objective of this work was to study nutritional strategies for decreasing methane production by ruminants fed tropical diets, combining in vitro and in vivo methods. the in vitro approach was used to evaluate the dose effect of condensed tannins (ct) contained in leaves of gliricidia sepium, leucaena leucocephala, and manihot esculenta (39, 75, and 92 g ct/kg dm, respectively) on methane production and ruminal fermentation characteristics. tannin-rich plants (trp) were incubated for 24 h alo ...201525568379
biogas production from vietnamese animal manure, plant residues and organic waste: influence of biomass composition on methane yield.anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. in developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. this study was therefore carried out with the objective of estimating the biogas production potential of typical vietnamese bioma ...201525557826
effect of the borax mass and pre-spray medium temperature on droplet size and velocity vector distributions of intermittently sprayed starchy solutions.spray coating technology has demonstrated great potential in the slow release fertilizers industry. the better understanding of the key spray parameters benefits both the environment and low cost coating processes. the use of starch based materials to coat the slow release fertilizers is a new development. however, the hydraulic spray jet breakup of the non-newtonian starchy solutions is a complex phenomenon and very little known. the aim of this research was to study the axial and radial distri ...201525557285
large-scale snp discovery through rna sequencing and snp genotyping by targeted enrichment sequencing in cassava (manihot esculenta crantz).cassava (manihot esculenta crantz) is one of the most important crop species being the main source of dietary energy in several countries. marker-assisted selection has become an essential tool in plant breeding. single nucleotide polymorphism (snp) discovery via transcriptome sequencing is an attractive strategy for genome complexity reduction in organisms with large genomes. we sequenced the transcriptome of 16 cassava accessions using the illumina hiseq platform and identified 675,559 est-der ...201425551642
field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load.production of cassava (manihot esculenta crantz), a food security crop in sub-saharan africa, is threatened by the spread of cassava brown streak disease (cbsd) which manifests in part as a corky necrosis in the storage root. it is caused by either of two virus species, cassava brown streak virus (cbsv) and ugandan cassava brown streak virus (ucbsv), resulting in up to 100% yield loss in susceptible varieties.201425526680
cassava (manihot esculenta krantz) genome harbors knox genes differentially expressed during storage root development.in plants, homeodomain proteins play a critical role in regulating various aspects of plant growth and development. knox proteins are members of the homeodomain protein family. the knox transcription factors have been reported from arabidopsis, rice, and other higher plants. the recent publication of the draft genome sequence of cassava (manihot esculenta krantz) has allowed a genome-wide search for m. esculenta knox (meknox) transcription factors and the comparison of these positively identifie ...201425526192
motor impairments induced by microinjection of linamarin in the dorsal hippocampus of wistar rats.cassava, also known as yuca or manioc (manihot esculenta crantz), is a staple food in tropical and subtropical regions since it is an important source of carbohydrates. nevertheless, it contains cyanogenic compounds including lotaustralin and linamarin, which have been shown by experimental models to affect brain structures such as the thalamus, the piriform cortex, the hippocampus, and others. these findings may explain the presence of such neurological diseases as konzo and tropical ataxic neu ...201625524044
predicting the potential invasion suitability of regions to cassava lacebug pests (heteroptera: tingidae: vatiga spp.).cassava (manihot esculenta crantz) is one of the most important staple crops for small farmers in the tropics, feeding about 800 million people worldwide. it is currently cultivated in south and central america, africa and asia. the genus vatiga is widespread throughout the neotropical region. its species are sympatric and feed exclusively on cassava. the main objectives of this paper are: (1) to assess the potential distribution of vatiga, one of the most relevant pests of cassava; (2) to proje ...201525523908
high-resolution linkage map and chromosome-scale genome assembly for cassava (manihot esculenta crantz) from 10 populations.cassava (manihot esculenta crantz) is a major staple crop in africa, asia, and south america, and its starchy roots provide nourishment for 800 million people worldwide. although native to south america, cassava was brought to africa 400-500 years ago and is now widely cultivated across sub-saharan africa, but it is subject to biotic and abiotic stresses. to assist in the rapid identification of markers for pathogen resistance and crop traits, and to accelerate breeding programs, we generated a ...201425504737
feeding and lying behavior of heat-stressed early lactation cows fed low fiber diets containing roughage and nonforage fiber sources.in addition to reduced nutrient intake, an environmental thermal load may directly affect milk yield in heat-stressed dairy cows. feeding and lying behaviors of early lactation cows fed low fiber diets containing neutral detergent fiber (ndf) from roughage and nonforage fiber sources (nffs) were investigated under summer conditions in thailand. immediately after calving, 30 multiparous cows (87.5% holstein × 12.5% sahiwal) were randomly allocated to dietary treatments for 63 d in a completely ra ...201525497805
acrylamide levels in selected colombian foods.acrylamide (aa) levels in conventional (n = 112) and traditional (n = 43) colombian foods were analysed by gas chromatography with mass spectrometry (gc/ms) detection. samples included: infant powdered formula, coffee and chocolate powders, corn snacks, bakery products and tuber-, meat- and vegetable-based foods. there was a wide variability in aa levels among different foods and within different brands of the same food, especially for coffee powder, breakfast cereals biscuits and french fries s ...025494681
nutritional composition of "gari" analog produced from cassava (manihot esculenta) and cocoyam (colocasia esculenta) tuber.physicochemical properties ofgari analog produced from coprocessing of cassava (manihot esculenta crantz) and cocoyam (colocasia esculenta) were investigated. cassava tuber and cocoyam were coprocessed at different percentages before frying separately. proximate composition, mineral content, antinutritional factors, and sensory evaluation of various samples were determined. the results showed that the moisture content of all the samples was in the same range (7.28 ± 0.30 to 7.78 ± 0.14%). the pr ...201425493189
two new terpenoids from the stems of manihot esculenta.a new eremophilane sesquiterpene, sporogen ao-2 (1), and a new beyerane diterpene, thecacorin c (2), together with two known compounds, longifoamide-b (3) and methylcholestane-3β,5α,6β-triol (4), were isolated from the stems of manihot esculenta. the structures of the two new compounds were determined by spectroscopic techniques (uv, ir, ms, 1d, and 2d nmr). antimicrobial assay showed that compound 3 possessed modest inhibitory effects on saphylococcus aureus and methicillin-resistant s.aureus, ...201525492303
correlated changes in structure and viscosity during gelatinization and gelation of tapioca starch granules.melting of native tapioca starch granules in aqueous pastes upon heating is observed in situ using simultaneous small- and wide-angle x-ray scattering (saxs/waxs) and solution viscometry. correlated structure and viscosity changes suggest closely associated amylose and amylopectin chains in the semicrystalline layers, and the release of amylose chains for enhanced solution viscosity occurs largely after melting of the semicrystalline structure. before melting, waxs results reveal mixed crystals ...201425485122
physicochemical properties of flours and starches derived from traditional indonesian tubers and roots.flours and starches isolated from traditional tubers and roots grown in indonesia have physical and chemical properties suitable for certain food applications. compared to other flour samples, cassava and canna flours contained the highest amount of total starch (ts) (77.4 and 77.1 %, respectively). taro starch had the lowest amount of ts among other starch samples with 75.4 %. the highest amount of amylose was observed from yam and canna flours (25.2 and 23.2 %, respectively). among starch samp ...201425477633
involvement of mir160/mir393 and their targets in cassava responses to anthracnose disease.cassava is a starchy root crop for food and industrial applications in many countries around the world. among the factors that affect cassava production, diseases remain the major cause of yield loss. cassava anthracnose disease is caused by the fungus colletotrichum gloeosporioides. severe anthracnose attacks can cause tip die-backs and stem cankers, which can affect the availability of planting materials especially in large-scale production systems. recent studies indicate that plants over- or ...201525462963
effect of granule organisation on the behaviour of starches in the nmmo (n-methyl morpholine n-oxide) solvent system.the response of starches of different botanical origin to heating in 78% n-methyl morpholine n-oxide (nmmo) is compared with their behaviour in water. for all starches studied an exothermic transition is obtained in the nmmo system rather than the endothermic transition in water. in nmmo the transition temperatures are lower for a-type starches (wheat, rice and tapioca) than the c-type starches (sago and pea) and also potato which has a b-type polymorph. observations using a hot stage microscope ...201525458278
form follows function in geminiviral minichromosome architecture.a comprehensive survey on the viral minichromosomes of the begomoviruses abutilon mosaic virus, tomato yellow leaf curl sardinia virus, african cassava mosaic virus, indian cassava mosaic virus (family geminiviridae) during the course of infections in nicotiana benthamiana is summarized. using optimized one-dimensional and two-dimensional gel systems combined with blot hybridization and a standardized evaluation, discrete and heterogeneous virus-specific signals with different dna forms were com ...201525445344
biodegradation of atrazine by three transgenic grasses and alfalfa expressing a modified bacterial atrazine chlorohydrolase gene.the widespread use of atrazine and other s-triazine herbicides to control weeds in agricultural production fields has impacted surface and groundwater in the united states and elsewhere. we previously reported the cloning, sequencing, and expression of six genes involved in the atrazine biodegradation pathway of pseudomonas sp. strain adp, which is initiated by atza, encoding atrazine chlorohydrolase. here we explored the use of enhanced expression of a modified bacterial atrazine chlorohydrolas ...201525432082
cassava (manihot esculenta crantz).genetic transformation of plants is an indispensable technique used for fundamental research and crop improvement. recent advances in cassava (manihot esculenta crantz) transformation have facilitated the effective generation of stably transformed cassava plants with favorable traits. agrobacterium-mediated transformation of friable, embryogenic callus has evolved to become the most widely used approach and has been adopted by research laboratories in africa. this procedure utilizes axillary mer ...201525416250
transcriptional analysis of south african cassava mosaic virus-infected susceptible and tolerant landraces of cassava highlights differences in resistance, basal defense and cell wall associated genes during infection.cassava mosaic disease is caused by several distinct geminivirus species, including south african cassava mosaic virus-[south africa:99] (sacmv). to date, there is limited gene regulation information on viral stress responses in cassava, and global transcriptome profiling in sacmv-infected cassava represents an important step towards understanding natural host responses to plant geminiviruses.201425412561
management of whitefly-transmitted viruses in open-field production systems.whiteflies are a key pest of crops in open-field production throughout the tropics and subtropics. this is due in large part to the long and diverse list of devastating plant viruses transmitted by these vectors. open-field production provides many challenges to manage these viruses and in many cases adequate management has not been possible. diseases caused by whitefly-transmitted viruses have become limiting factors in open-field production of a wide range of crops, i.e., bean golden mosaic di ...201425410102
activity and abundance of methane-oxidizing bacteria in secondary forest and manioc plantations of amazonian dark earth and their adjacent soils.the oxidation of atmospheric ch4 in upland soils is mostly mediated by uncultivated groups of microorganisms that have been identified solely by molecular markers, such as the sequence of the pmoa gene encoding the β-subunit of the particulate methane monooxygenase enzyme. the objective of this work was to compare the activity and diversity of methanotrophs in amazonian dark earth soil (ade, hortic anthrosol) and their adjacent non-anthropic soil. secondly, the effect of land use in the form of ...201425374565
encapsulation of black carrot juice using spray and freeze drying.black carrot juice extracted using pectinase enzyme was encapsulated in three different carrier materials (maltodextrin 20de, gum arabic and tapioca starch) using spray drying at four inlet temperatures (150, 175, 200 and 225 ℃) and freeze drying at a constant temperature of - 53 ℃ and vacuum of 0.22-0.11 mbar with the constant feed mixture. the products were analyzed for total anthocyanin content, antioxidant activity, water solubility index, encapsulation efficiency and total colour change. fo ...201525367889
evaluation of coprocessed disintegrants produced from tapioca starch and mannitol in orally disintegrating paracetamol tablet.the study evaluated two novel coprocessed excipients (with two methods) as disintegrants in an orally disintegrating paracetamol tablet formulation. the tablets produced were assessed for mechanical properties with the use of friability and tensile strength while the release properties were assessed with wetting time, water absorption ratio, disintegration time and dissolution profile. the results obtained showed that the methods of coprocessing and disintegrant incorporation influenced the acti ...201425362809
engineering geminivirus resistance in jatropha curcus.jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. however, j. curcus is susceptible to the geminivirus indian cassava mosaic virus (icmv), and frequent viral disease outbreaks severely limit productivity. therefore the development of j. curcus to carry on durable virus resistance remains crucial and poses a major biotechnological challenge.201425352912
unexpected outcomes of thai cassava trade: a case of global complexity and local unsustainability.tracing the thai cassava (manihot esculenta) trade network, between 1960 and 2000, offers a compelling example of global complexity at work. the emergence of thailand's dominance of world export markets caught the world by surprise. the opening up of a european market for cassava was supposed to be met by brazilian and indonesian producers. instead, thailand took over the market by 1975. several factors facilitated this emergence including: entrepreneurial diasporic networks of thai-chinese trad ...200825328444
physicochemical properties of cassava starch oxidized by sodium hypochlorite.in this work, cassava starch was modified by treatment with sodium hypochlorite (naclo) at different concentrations (0.8, 2.0 and 5.0 % of active chlorine) and selected physicochemical properties of the oxidized starches were investigated. the native and modified samples were evaluated considering moisture, carboxyl content, apparent viscosity, susceptibility to syneresis, mid-infrared spectroscopy and crystallinity index. the treatment with naclo resulted in alterations in carboxyl content of t ...201425328206
construction and comparison of gene co-expression networks shows complex plant immune responses.gene co-expression networks (gcns) are graphic representations that depict the coordinated transcription of genes in response to certain stimuli. gcns provide functional annotations of genes whose function is unknown and are further used in studies of translational functional genomics among species. in this work, a methodology for the reconstruction and comparison of gcns is presented. this approach was applied using gene expression data that were obtained from immunity experiments in arabidopsi ...201425320678
corrin-based chemosensors for the assured detection of endogenous cyanide.cassava (manihot esculenta crantz) is a staple food for more than 500 million people, especially in africa and south america. however, its consumption bears risks as it contains cyanogenic glycosides that convert enzymatically to toxic cyanide during cell damage. to avoid serious health problems by unintentional cyanide intake, this dangerous product of decomposition must be removed before consumption. for monitoring such food processing procedures and for controlling the quality and safety of c ...201525317920
evolutionary liberties of the abutilon mosaic virus cluster.two new strains of abutilon mosaic virus (abmv; geminiviridae) from germany (stuttgart) and france (paris) have been characterized by circomics, direct pyrosequencing of rolling circle amplification (rca) products, as well as conventional cloning and sanger sequencing. rca combined with an analysis of restriction fragment length polymorphisms confirmed the completeness of the sequence determination and a close relationship of both isolates for dna a with 99 % nucleotide sequence identity. phylog ...201525315632
a standardized extract of ginkgo biloba prevents locomotion impairment induced by cassava juice in wistar rats.the long-term consumption of cassava (manihot esculenta crantz) juice produce neurotoxic effects in the rat, characterized by an increased motor activity in the open field test and presence of uncoordinated swim (i.e., lateral swimming), in the swim test; which has been associated with damage in the hippocampus (ca1). on the other hand, flavonoids content in the ginkgo biloba extract has been reported to produces neuroprotective effects at experimental level; therefore we hypothesized that g. bi ...201425309441
impact of edible chitosan-cassava starch coatings enriched with lippia gracilis schauer genotype mixtures on the shelf life of guavas (psidium guajava l.) during storage at room temperature.the effect of edible chitosan-cassava starch (ch-cs) coatings containing a mixture of lippia gracilis schauer genotypes (eom) on the shelf life of guavas during storage at room temperature for 10 days was studied. sixteen formulations were prepared with a range of chitosan and essential oil mixtures concentrations, and the in vitro antimicrobial activity was tested. formulations containing 2.0% cassava starch, 2.0% chitosan and 1.0%, 2.0% or 3.0% eom were most effective in inhibiting the growth ...201525308649
genome sequence of erinnyis ello granulovirus (erelgv), a natural cassava hornworm pesticide and the first sequenced sphingid-infecting betabaculovirus.cassava (manihot esculenta) is the basic source for dietary energy of 500 million people in the world. in brazil, erinnyis ello ello (lepidoptera: sphingidae) is a major pest of cassava crops and a bottleneck for its production. in the 1980s, a naturally occurring baculovirus was isolated from e. ello larva and successfully applied as a bio-pesticide in the field. here, we described the structure, the complete genome sequence, and the phylogenetic relationships of the first sphingid-infecting be ...201425280947
enzymatic and acid conversion of new starches from improved orphan crops: prospects for renewable materials uses in food and non-food industries.the enzymatic and acid hydrolysis have converted eight new starches into a range of chain lengths mainly including glucose, maltose, and maltodextrins as observed on tlc plates, irrespective to the starch variety and treatment. results of the enzymatic hydrolysis have highlighted the possibility of the use of v4 and v64, which can be labelled as "dietary fibres", to enhance the organoleptic qualities of foods and for fibre fortification of low-calorie products. concerning v66 and v69, they have ...201425279291
diversity and localization of bacterial endosymbionts from whitefly species collected in brazil.whiteflies (hemiptera: aleyrodidae) are sap-sucking insect pests, and some cause serious damage in agricultural crops by direct feeding and by transmitting plant viruses. whiteflies maintain close associations with bacterial endosymbionts that can significantly influence their biology. all whitefly species harbor a primary endosymbiont, and a diverse array of secondary endosymbionts. in this study, we surveyed 34 whitefly populations collected from the states of sao paulo, bahia, minas gerais an ...201425259930
improvement of the amides forming capacity of the arylacetonitrilase from pseudomonas fluorescens ebc191 by site-directed mutagenesis.the influence of different amino acid substitutions in the nitrilase from pseudomonas fluorescens ebc191 (nita) on the catalytical activity and the ability to form amides was investigated. the enzyme variant glu137ala was constructed because glutamate residues homologous to glu137 are highly conserved among different members of the nitrilase superfamily and it has been suggested that these residues are indispensable for the hydrolysis of amides by enzymes belonging to the nitrilase superfamily. ...201525248440
characterisation and evaluation of a novel feedstock, manihot glaziovii, muell. arg, for production of bioenergy carriers: bioethanol and biogas.the objective of this study was to characterise and evaluate a wild inedible cassava species, manihot glaziovii as feedstock for bioenergy production. tubers obtained from 3 different areas in tanzania were characterised and evaluated for bioethanol and biogas production. these bioenergy carriers were produced both separately and sequentially and their energy values evaluated based on these two approaches. composition analysis demonstrated that m. glaziovii is a suitable feedstock for both bioet ...201425237774
structural and functional analysis of hydroxynitrile lyase from baliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics.hydroxynitrile lyases (hnls) catalyze degradation of cyanohydrins to hydrogen cyanide and the corresponding ketone or aldehyde. hnls can also catalyze the reverse reaction, i.e., synthesis of cyanohydrins. although several crystal structures of s-selective hydroxynitrile lyases (s-hnls) have been reported, it remains unknown whether and how dynamics at the active site of s-hnls influence their broad substrate specificity and affinity. in this study, we analyzed the structure, dynamics and functi ...201425220808
immune response and disease resistance of shrimp fed biofloc grown on different carbon sources.the objective of this study was to document the immunological effects of growing shrimp in biofloc systems. the experiment consisted of four types of biofloc systems in which bioflocs were produced by daily supplementation of four different carbon sources, i.e. molasses, tapioca, tapioca-by-product, and rice bran, at an estimated c/n ratio of 15 and a control system without any organic carbon addition. each biofloc system was stocked with pacific white shrimp (litopenaeus vannamei) juveniles tha ...201425218685
nutrient composition and protein quality evaluation of eri silkworm (samia ricinii) prepupae and pupae.eri silkworm (samia ricinii) is a traditional source of food in northeast india, where it is grown primarily for silk and food uses. nutrient analysis showed that the proximate composition of eri silkworm prepupae and pupae grown on either castor or tapioca were comparable and it was a good source of protein (16g%), fat (8g%) and minerals. the amino acid scores of eri prepupae and pupae protein were 99 and 100, respectively, with leucine as the limiting amino acid in both cases. net protein util ...201125212147
molecular cloning and expression of a novel myb transcription factor gene in rubber tree.myb family proteins regulate a variety of cellular processes in plants. tapping panel dryness (tpd) in rubber tree (hevea brasiliensis muell. arg.) affects latex biosynthesis and causes serious losses to rubber producers. in this study, a novel sant/myb transcription factor gene down-regulated in tpd rubber tree, named as hbsm1, was isolated from rubber tree. the complete hbsm1 open reading frame (orf) was 948 bp in length. the deduced hbsm1 protein is 315 amino acids. hbsm1 belonged to 1rmyb su ...201425195053
thermodynamic analysis of sorption isotherms of cassava (manihot esculenta).sorption isotherms of cassava were determined experimentally using a static gravimetric method at 30, 45 and 60 °c and within the range of 0.10-0.90 water activity. at a constant water activity, equilibrium moisture content decreased with increasing temperature. the equilibrium moisture content increased with increasing water activity at a given temperature. the experimental results were modelled using seven sorption models using non-linear regression technique. results demonstrated that the gab ...201425190827
a high-throughput method for screening of aspergillus niger mutants with high transglycosylation activity by detecting non-fermentable reducing sugar.a novel high-throughput method was established for rapid screening of large numbers of aspergillus niger mutants with high transglucosylation activity by exploiting that yeast can hardly hydrolyze isomaltooligosaccharides (imo). supernatants of a. niger fermentation were incubated with saccharomyces cerevisiae to remove glucose and maltose, and the remaining non-reducing sugars, which is positively correlated with the amount of imo, the products of transglucosylation reaction, were used as indic ...201125187152
genetic parameters and prediction of genotypic values for root quality traits in cassava using reml/blup.the aim of this study was to estimate the genetic parameters and predict the genotypic values of root quality traits in cassava (manihot esculenta crantz) using restricted maximum likelihood (reml) and best linear unbiased prediction (blup). a total of 471 cassava accessions were evaluated over two years of cultivation. the evaluated traits included amylose content (aml), root dry matter (dmc), cyanogenic compounds (cyc), and starch yield (styi). estimates of the individual broad-sense heritabil ...201425177949
potato virus x-based expression vectors are stabilized for long-term production of proteins and larger inserts.plus-strand rna viruses such as potato virus x (pvx) are often used as high-yielding expression vectors in plants, because they tolerate extra transgene insertion and expression without disrupting normal virus functions. however, sequence redundancy due to promoter duplication often leads to genetic instability. although heterologous subgenomic promoter-like sequences (sgps) have been successfully used in tobacco mosaic virus vectors, only homologous sgp duplications have been used in pvx vector ...201425171768
utilization of by-products derived from bioethanol production process for cost-effective production of lactic acid.the by-products of bioethanol production such as thin stillage (ts) and condensed distillers solubles (cds) were used as a potential nitrogen source for economical production of lactic acid. the effect of those by-products and their concentrations on lactic acid fermentation were investigated using lactobacillus paracasei chb2121. approximately, 6.7 g/l of yeast extract at a carbon source to nitrogen source ratio of 15 was required to produce 90 g/l of lactic acid in the medium containing 100 g/ ...201425163666
development of a cassava core collection based on single nucleotide polymorphism markers.single nucleotide polymorphism (snp) markers were used in the largest cassava (manihot esculenta crantz) germplasm collection from brazil to develop core collections based on the maximization strategy. subsets with 61, 64, 84, 128, 256, and 384 cassava accessions were selected and named poheu, mst64, poran, mst128, mst256, and mst384, respectively. all the 798 alleles identified by 402 snp markers in the entire collection were captured in all core collections. only small alterations in the diver ...201425158266
sri lankan cassava mosaic virus replication associated protein (rep) triggers transposition of is426 in agrobacterium.we report a high rate of is426 transposition in agrobacterium tumefaciens in the presence of the sri lankan cassava mosaic virus (slcmv) replication associated protein gene (rep). upon conjugal transfer of the binary plasmid pcam-slcmv-rep with the slcmv rep gene in the sense orientation under the transcriptional control of the cauliflower mosaic virus (camv) 35s promoter into the a. tumefaciens vir helper strain eha105, the binary plasmid size increased in all 15 transconjugants studied. southe ...201425135797
rnaseq analysis of cassava reveals similar plant responses upon infection with pathogenic and non-pathogenic strains of xanthomonas axonopodis pv. manihotis.an rnaseq-based analysis of the cassava plants inoculated with xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a tale. cassava bacterial blight, a disease caused by the gram-negative bacterium xanthomonas axonopodis pv. manihotis (xam), is a major limitation to cassava production worldwide and especially in developing countries. the molecular mechanisms underlying cassava susceptibilit ...201425120000
optimization of ingredients for noodle preparation using response surface methodology.in the present investigation, the composite flour combinations using whole wheat flour (x1), tapioca flour (x2) and defatted soy flour (x3) for the noodle preparation were made through central composite rotatable design (ccrd). the analyzed responses were bulk density (bd), proximate composition, water uptake ratio (wur), cooking volume expansion (ve), water absorption capacity (wac), swelling capacity (sc), gruel solid loss (gsl), and sensory characteristics. a second order quadratic polynomial ...201425114340
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