Publications
Title | Abstract | Year Filter | PMID(sorted descending) Filter |
---|
fruiting branch k(+) level affects cotton fiber elongation through osmoregulation. | potassium (k) deficiency in cotton plants results in reduced fiber length. as one of the primary osmotica, k(+) contributes to an increase in cell turgor pressure during fiber elongation. therefore, it is hypothesized that fiber length is affected by k deficiency through an osmotic pathway, so in 2012 and 2013, an experiment was conducted to test this hypothesis by imposing three potassium supply regimes (0, 125, 250 kg k ha(-1)) on a low-k-sensitive cultivar, siza 3, and a low-k-tolerant cultiv ... | 2016 | 26834777 |
comparative fiber property and transcriptome analyses reveal key genes potentially related to high fiber strength in cotton (gossypium hirsutum l.) line md52ne. | individual fiber strength is an important quality attribute that greatly influences the strength of the yarn spun from cotton fibers. fiber strength is usually measured from bundles of fibers due to the difficulty of reliably measuring strength from individual cotton fibers. however, bundle fiber strength (bfs) is not always correlated with yarn strength since it is affected by multiple fiber properties involved in fiber-to-fiber interactions within a bundle in addition to the individual fiber s ... | 2016 | 26833213 |
small interfering rnas from bidirectional transcripts of ghmml3_a12 regulate cotton fiber development. | natural antisense transcripts (nats) are commonly observed in eukaryotic genomes, but only a limited number of such genes have been identified as being involved in gene regulation in plants. in this research, we investigated the function of small rna derived from a nat in fiber cell development. using a map-based cloning strategy for the first time in tetraploid cotton, we cloned a naked seed mutant gene (n1 ) encoding a mybmixta-like transcription factor 3 (mml3)/ghmyb25-like in chromosome a12, ... | 2016 | 26832840 |
functional characterization of a dihydroflavanol 4-reductase from the fiber of upland cotton (gossypium hirsutum). | dihydroflavanol 4-reductase (dfr) is a key later enzyme involved in two polyphenols' (anthocyanins and proanthocyanidins (pas)) biosynthesis, however it is not characterized in cotton yet. in present reports, a dfr cdna homolog (designated as ghdfr1) was cloned from developing fibers of upland cotton. silencing ghdfr1 in cotton by virus-induced gene silencing led to significant decrease in accumulation of anthocyanins and pas. more interestingly, based on lc-ms analysis, two pa monomers, (-)-epi ... | 2016 | 26821011 |
field-based high-throughput plant phenotyping reveals the temporal patterns of quantitative trait loci associated with stress-responsive traits in cotton. | the application of high-throughput plant phenotyping (htpp) to continuously study plant populations under relevant growing conditions creates the possibility to more efficiently dissect the genetic basis of dynamic adaptive traits. toward this end, we employed a field-based htpp system that deployed sets of sensors to simultaneously measure canopy temperature, reflectance, and height on a cotton (gossypium hirsutum l.) recombinant inbred line mapping population. the evaluation trials were conduc ... | 2016 | 26818078 |
a cotton mirna is involved in regulation of plant response to salt stress. | the present study functionally identified a new microrna (microrna ovual line 5, mirnvl5) with its target gene ghchr from cotton (gossypium hirsutum). the sequence of mirnvl5 precursor is 104 nt long, with a well developed secondary structure. ghchr contains two dc1 and three phd cys/his-rich domains, suggesting that ghchr encodes a zinc-finger domain-containing transcription factor. mirnvl5 and ghchr express at various developmental stages of cotton. under salt stress (50-400 mm nacl), mirnvl5 ... | 2016 | 26813144 |
influence of dual-bt protein corn on bollworm, helicoverpa zea (boddie), survivorship on bollgard ii cotton. | similar cry proteins are expressed in both bt corn, zea mays l., and cotton, gossypium hirsutum (l.), commercial production systems. at least one generation of corn earworm, helicoverpa zea (boddie), completes development on field corn in the mid-south before dispersing across the landscape into other crop hosts like cotton. a concern is that bt corn hybrids may result in selection for h. zea populations with a higher probability of causing damage to bt cotton. the objective of this study was to ... | 2016 | 26809264 |
two-dimensional gel electrophoresis-based analysis provides global insights into the cotton ovule and fiber proteomes. | proteomic analysis of upland cotton was performed to profile the global detectable proteomes of ovules and fibers using two-dimensional electrophoresis (2de). a total of 1,203 independent protein spots were collected from representative 2de gels, which were digested with trypsin and identified by matrix-assisted laser desorption and ionization-time-offlight/ time-of-flight (maldi-tof/tof) mass spectrometry. the mass spectrometry or tandem mass spectrometry (ms or ms/ms) data were then searched a ... | 2016 | 26803300 |
cotton ghmyb7 is predominantly expressed in developing fibers and regulates secondary cell wall biosynthesis in transgenic arabidopsis. | the secondary cell wall in mature cotton fibers contains over 90% cellulose with low quantities of xylan and lignin. however, little is known regarding the regulation of secondary cell wall biosynthesis in cotton fibers. in this study, we characterized an r2r3-myb transcription factor, ghmyb7, in cotton. ghmyb7 is expressed at a high level in developing fibers and encodes a myb protein that is targeted to the cell nucleus and has transcriptional activation activity. ectopic expression of ghmyb7 ... | 2016 | 26803299 |
genome-wide transcriptomic and proteomic analyses of bollworm-infested developing cotton bolls revealed the genes and pathways involved in the insect pest defence mechanism. | cotton bollworm, helicoverpa armigera, is a major insect pest that feeds on cotton bolls causing extensive damage leading to crop and productivity loss. in spite of such a major impact, cotton plant response to bollworm infection is yet to be witnessed. in this context, we have studied the genome-wide response of cotton bolls infested with bollworm using transcriptomic and proteomic approaches. further, we have validated this data using semi-quantitative real-time pcr. comparative analyses have ... | 2016 | 26799171 |
relative fitness of helicoverpa armigera (lepidoptera: noctuidae) on seven host plants: a perspective for ipm in brazil. | the cotton bollworm helicoverpa armigera (hübner) (lepidoptera: noctuidae) is a widespread pest of many cultivated and wild plants in europe, africa, asia, and australia. in 2013, this species was reported in brazil, attacking various host crops in the midwestern and northeastern regions of the country and is now found countrywide. aiming to understand the effects of different host plants on the life cycle of h. armigera, we selected seven species of host plants that mature in different seasons ... | 2016 | 26798139 |
online co2 and h2 o oxygen isotope fractionation allows estimation of mesophyll conductance in c4 plants, and reveals that mesophyll conductance decreases as leaves age in both c4 and c3 plants. | mesophyll conductance significantly, and variably, limits photosynthesis but we currently have no reliable method of measurement for c4 plants. an online oxygen isotope technique was developed to allow quantification of mesophyll conductance in c4 plants and to provide an alternative estimate in c3 plants. the technique is compared to an established carbon isotope method in three c3 species. mesophyll conductance of c4 species was similar to that in the c3 species measured, and declined in both ... | 2016 | 26778088 |
frankliniella fusca resistance to neonicotinoid insecticides: an emerging challenge for cotton pest management in the eastern united states. | over the past two decades, neonicotinoid seed treatments have become the primary method to manage tobacco thrips, frankliniella fusca hinds, on seedling cotton. because this insect is highly polyphagous and the window of insecticide exposure is short, neonicotinoid resistance was expected to pose a minimal risk. however, reports of higher than expected f. fusca seedling damage in seed-treated cotton fields throughout the mid-south and southeast us production regions suggested neonicotinoid resis ... | 2016 | 26775623 |
genetic diversity of the two commercial tetraploid cotton species in the gossypium diversity reference set. | a diversity reference set has been constructed for the gossypium accessions in the us national cotton germplasm collection to facilitate more extensive evaluation and utilization of accessions held in the collection. a set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid gossypium accessions representative of the range of diversity of the improved and wild accessions of g. hirsutum and g. barbadense. the reference set contained 410 g. barbad ... | 2016 | 26774060 |
functional divergence of ghcfe5 homoeologs revealed in cotton fiber and arabidopsis root cell development. | in ghcfe5 homoeologs, ghcfe5d interacted with more actin homologs and stronger interaction activity than ghcfe5a. ghcfe5d - but not ghcfe5a -overexpression severely disrupted actin cytoskeleton organization and significantly suppressed cell elongation. homoeologous genes are common in polyploid plants; however, their functional divergence is poorly elucidated. allotetraploid upland cotton (gossypium hirsutum, aadd) is the most widely cultivated cotton; accounting for more than 90 % of the world' ... | 2016 | 26759310 |
identification and characterization of micrornas in the plant parasitic root-knot nematode meloidogyne incognita using deep sequencing. | the root-knot nematode meloidogyne incognita is among the most damaging plant-parasitic pests of several crops including cotton (gossypium hirsutum) and tomato (lycopersicon escultentum). recently, a genome has become available for m. incognita, which greatly facilitates investigation of the interactions between m. incognita and its plant hosts at the molecular level and enables formation of hypotheses concerning development at the cellular level. micrornas (mirnas) are a class of small rna mole ... | 2016 | 26743520 |
resistance to glufosinate is proportional to phosphinothricin acetyltransferase expression and activity in libertylink(®) and widestrike(®) cotton. | insertion of the gene encoding phosphinothricin acetyltransferase (pat) has resulted in cotton plants resistant to the herbicide glufosinate. however, the lower expression and commensurate reduction in pat activity is a key factor in the low level of injury observed in the widestrike(®) cotton and relatively high level of resistance observed in libertylink(®) cotton. libertylink(®) cotton cultivars are engineered for glufosinate resistance by overexpressing the bar gene that encodes phosphinothr ... | 2016 | 26733464 |
breeding potential of introgression lines developed from interspecific crossing between upland cotton (gossypium hirsutum) and gossypium barbadense: heterosis, combining ability and genetic effects. | upland cotton (gossypium hirstum l.), which produces more than 95% of the world natural cotton fibers, has a narrow genetic base which hinders progress in cotton breeding. introducing germplasm from exotic sources especially from another cultivated tetraploid g. barbadense l. can broaden the genetic base of upland cotton. however, the breeding potential of introgression lines (ils) in upland cotton with g. barbadense germplasm integration has not been well addressed. this study involved six ils ... | 2016 | 26730964 |
partial dominance, overdominance, epistasis and qtl by environment interactions contribute to heterosis in two upland cotton hybrids. | based on two recombinant inbred line (ril) populations, two corresponding backcross (bc) populations were constructed to elucidate the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). the yield, and yield components, of these populations were evaluated in three environments. at the single-locus level, 78 and 66 quantitative trait loci (qtl) were detected using composite interval mapping in ril and bc populations, respectively, and 29 qtl were identified based on mid-parental ... | 2015 | 26715091 |
atrav1 and atrav2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering. | there is a longstanding problem of an inverse relationship between cotton fiber qualities versus high yields. to better understand drought stress signaling and adaptation in cotton (gossypium hirsutum) fiber development, we expressed the arabidopsis transcription factors related_to_aba-insensitive3/viviparous1/(rav1) and atrav2, which encode apetala2-basic3 domain proteins shown to repress transcription of flowering_locus_t (ft) and to promote stomatal opening cell-autonomously. in three years o ... | 2015 | 26706061 |
polyamine and its metabolite h2o2 play a key role in the conversion of embryogenic callus into somatic embryos in upland cotton (gossypium hirsutum l.). | the objective of this study was to increase understanding about the mechanism by which polyamines (pas) promote the conversion of embryogenic calli (ec) into somatic embryos in cotton (gossypium hirsutum l.). we measured the levels of endogenous pas and h2o2, quantified the expression levels of genes involved in the pas pathway at various stages of cotton somatic embryogenesis (se), and investigated the effects of exogenous pas and h2o2 on differentiation and development of ec. putrescine (put), ... | 2015 | 26697030 |
delineating the glycoproteome of elongating cotton fiber cells. | the data presented here delineates the glycoproteome component in the elongating cotton fiber cells attained using complementary proteomic approaches followed by protein and n-linked glycosylation site identification (kumar et al., 2013) [1]. utilizing species specific protein sequence databases in proteomic approaches often leads to additional information that may not be obtained using cross-species databases. in this context we have reanalyzed our glycoproteome dataset with the gossypium arbor ... | 2015 | 26693171 |
promoter isolation and characterization of ghao-like1, a gossypium hirsutum gene similar to multicopper oxidases that is highly expressed in reproductive organs. | cotton is one of the most economically important cultivated crops. it is the major source of natural fiber for the textile industry and an important target for genetic modification for both biotic stress and herbicide tolerance. therefore, the characterization of genes and regulatory regions that might be useful for genetic transformation is indispensable. the isolation and characterization of new regulatory regions is of great importance to drive transgene expression in genetically modified cro ... | 2016 | 26692462 |
microarray-based large scale detection of single feature polymorphism in gossypium hirsutum l. | microarrays offer an opportunity to explore the functional sequence polymorphism among different cultivars of many crop plants. the affymetrix microarray expression data of five genotypes of gossypium hirsutum l. at six different fibre developmental stages was used to identify single feature polymorphisms (sfps). the background corrected and quantile-normalized log2 intensity values of all probes of triplicate data of each cotton variety were subjected to sfps call by using sam procedure in r la ... | 2015 | 26690522 |
transposable elements play an important role during cotton genome evolution and fiber cell development. | transposable elements (tes) usually occupy largest fractions of plant genome and are also the most variable part of the structure. although traditionally it is hallmarked as "junk and selfish dna", today more and more evidence points out te's participation in gene regulations including gene mutation, duplication, movement and novel gene creation via genetic and epigenetic mechanisms. the recently sequenced genomes of diploid cottons gossypium arboreum (aa) and gossypium raimondii (dd) together w ... | 2016 | 26687725 |
constitutive expression of the tzs gene from agrobacterium tumefaciens virg mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation. | key message : virg mutant strains of a nopaline type of agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. constitutive cytokinin expression from the tzs gene in the virg mutant strains is responsible for the improvement. strains of agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. two disarmed a. tumefaciens nopaline strains with either a virgn54d constitutively active mutation or virgi77v ... | 2016 | 26650837 |
the genome sequence of sea-island cotton (gossypium barbadense) provides insights into the allopolyploidization and development of superior spinnable fibres. | gossypium hirsutum contributes the most production of cotton fibre, but g. barbadense is valued for its better comprehensive resistance and superior fibre properties. however, the allotetraploid genome of g. barbadense has not been comprehensively analysed. here we present a high-quality assembly of the 2.57 gigabase genome of g. barbadense, including 80,876 protein-coding genes. the double-sized genome of the a (or at) (1.50 gb) against d (or dt) (853 mb) primarily resulted from the expansion o ... | 2015 | 26634818 |
partial dominance, overdominance and epistasis as the genetic basis of heterosis in upland cotton (gossypium hirsutum l.). | determination of genetic basis of heterosis may promote hybrid production in upland cotton (gossypium hirsutum l.). this study was designed to explore the genetic mechanism of heterosis for yield and yield components in f2: 3 and f2: 4 populations derived from a hybrid 'xinza no. 1'. replicated yield field trials of the progenies were conducted in 2008 and 2009. phenotypic data analyses indicated overdominance in f1 for yield and yield components. additive and dominance effects at single-locus l ... | 2015 | 26618635 |
transformation and evaluation of cry1ac+cry2a and gtgene in gossypium hirsutum l. | more than 50 countries around the globe cultivate cotton on a large scale. it is a major cash crop of pakistan and is considered "white gold" because it is highly important to the economy of pakistan. in addition to its importance, cotton cultivation faces several problems, such as insect pests, weeds, and viruses. in the past, insects have been controlled by insecticides, but this method caused a severe loss to the economy. however, conventional breeding methods have provided considerable break ... | 2015 | 26617613 |
virus-induced gene silencing in cultivated cotton (gossypium spp.) using tobacco rattle virus. | the study described here has optimized the conditions for virus-induced gene silencing (vigs) in three cultivated cotton species (gossypium hirsutum, g. arboreum, and g. herbaceum) using a tobacco rattle virus (trv) vector. the system was used to silence the homolog of the arabidopsis thaliana chloroplastos alterados 1 (atcla1) gene, involved in chloroplast development, in g. herbaceum, g. arboreum, and six commercial g. hirsutum cultivars. all plants inoculated with the trv vector to silence cl ... | 2016 | 26603442 |
susceptibility to insecticides and activities of glutathione s-transferase and esterase in populations of lygus lineolaris (hemiptera: miridae) in mississippi. | lygus lineolaris (palisot de beauvois) is a serious pest of cotton (gossypium hirsutum l.) in mississippi, particularly in the delta region. this may be due to decreased insecticide susceptibility in that region. research has revealed populations of l. lineolaris in the delta region with high levels of insecticide resistance; however, comparisons with populations in the remainder of the state are limited. | 2016 | 26598042 |
pseudomonas induces salinity tolerance in cotton (gossypium hirsutum) and resistance to fusarium root rot through the modulation of indole-3-acetic acid. | abiotic stresses cause changes in the balance of phytohormones in plants and result in inhibited root growth and an increase in the susceptibility of plants to root rot disease. the aim of this work was to ascertain whether microbial indole-3-acetic acid (iaa) plays a role in the regulation of root growth and microbially mediated control of root rot of cotton caused by fusarium solani. seed germination and seedling growth were improved by both nacl and mg2so4 (100 mm) solutions when treated with ... | 2015 | 26587006 |
stable transformation and expression of ghexpa8 fiber expansin gene to improve fiber length and micronaire value in cotton. | cotton fiber is multigenic trait controlled by number of genes. previous studies suggest that one of these genes may be responsible for switching cotton fiber growth on and off to influence the fiber quality produced from a cotton seed. in the present study, the gossypium hirsutum ghexpa8 fiber expansin gene was introduced into local cotton variety niab 846 by using an agrobacterium-mediated gene transformation. the neomycin phosphotransferase (nptii) gene was used as a selection marker for scre ... | 2015 | 26583018 |
transcriptome analysis of cotton (gossypium hirsutum l.) genotypes that are susceptible, resistant, and hypersensitive to reniform nematode (rotylenchulus reniformis). | reniform nematode is a semi-endoparasitic nematode species causing significant yield loss in numerous crops, including cotton (gossypium hirsutum l.). an rna-sequencing analysis was conducted to measure transcript abundance in reniform nematode susceptible (dp90 & sg747), resistant (barbren-713), and hypersensitive (lonren-1) genotypes of cotton (gossypium hirsutum l.) with and without reniform nematode infestation. over 90 million trimmed high quality reads were assembled into 84,711 and 80, 35 ... | 2015 | 26571375 |
a and d genomes spatial separation at somatic metaphase in tetraploid cotton: evidence for genomic disposition in a polyploid plant. | chromosomal dispositions were analyzed on the metaphase plate of tetraploid cotton (aadd). at metaphase, the two subgenomes, a and d, were separated in a radial pattern in which the small d subgenome chromosomes tended to concentrate at the center and the large a subgenome chromosomes were scattered about the periphery on the metaphase plate. although the ordered chromosome arrangement was disturbed in an artificial hexaploid (aaddgg), the separation pattern could be recovered after the majority ... | 2015 | 26568399 |
integrated mapping and characterization of the gene underlying the okra leaf trait in gossypium hirsutum l. | diverse leaf morphology has been observed among accessions of gossypium hirsutum, including okra leaf, which has advantages and disadvantages in cotton production. the okra leaf locus has been mapped to chromosome 15 of the dt subgenome, but the underlying gene has yet to be identified. in this study, we used a combination of targeted association analysis, f2 population-based fine mapping, and comparative sequencing of orthologues to identify a candidate gene underlying the okra leaf trait in g. ... | 2016 | 26567355 |
functional characterization of ghsoc1 and ghmads42 homologs from upland cotton (gossypium hirsutum l.). | in arabidopsis flowering pathway, mads-box genes encode transcription factors, with their structures and functions highly conserved in many species. in our study, two mads-box genes ghsoc1 and ghmads42 (gossypium hirsutum l.) were cloned from upland cotton ccri36 and transformed into arabidopsis. ghsoc1 was additionally transformed into upland cotton. comparative analysis demonstrated sequence conservation between ghsoc1 and ghmads42 and genes of other plant species. tissue-specific expression a ... | 2016 | 26566835 |
spectral reflectance properties of gossypium hirsutum leaves after heavy metal toxicity. | the study demonstrated a link between heavy metal induced stress and optical properties of the leaves of gossypium hirsutum. this work was conducted using the pot culture experiment. three replications and four concentrations of chromium (5%, 10%, 20% and 35%) with a control to assess the growth of plants were used. this experiment was conducted till 80 days. on 80th day the experiment was terminated and the leaves were plucked and air-dried. portion of the air-dried, powdered cotton leaves was ... | 2014 | 26563065 |
the cotton wrky gene ghwrky41 positively regulates salt and drought stress tolerance in transgenic nicotiana benthamiana. | wrky transcription factors constitute a very large family of proteins in plants and participate in modulating plant biological processes, such as growth, development and stress responses. however, the exact roles of wrky proteins are unclear, particularly in non-model plants. in this study, gossypium hirsutum wrky41 (ghwrky41) was isolated and transformed into nicotiana benthamiana. our results showed that overexpression of ghwrky41 enhanced the drought and salt stress tolerance of transgenic ni ... | 2015 | 26562293 |
stable integration and expression of a cry1ia gene conferring resistance to fall armyworm and boll weevil in cotton plants. | boll weevil is a serious pest of cotton crop. effective control involves applications of chemical insecticides, increasing the cost of production and environmental pollution. the current genetically modified bt crops have allowed great benefits to farmers but show activity limited to lepidopteran pests. this work reports on procedures adopted for integration and expression of a cry transgene conferring resistance to boll weevil and fall armyworm by using molecular tools. | 2016 | 26558603 |
molecular cloning, expression analysis and subcellular localization of a transparent testa 12 ortholog in brown cotton (gossypium hirsutum l.). | transparent testa 12 (tt12) is a kind of transmembrane transporter of proanthocyanidins (pas), which belongs to a membrane-localized multidrug and toxin efflux (mate) family, but the molecular basis of pas transport is still poorly understood. here, we cloned a full-length tt12 cdna from the fiber of brown cotton (gossypium hirsutum), named ghtt12 (genbank accession no. kf240564), which comprised 1733 bp with an open reading frame (orf) of 1503 bp and encoded a putative protein containing 500 am ... | 2016 | 26548815 |
analysis of upland cotton (gossypium hirsutum) response to verticillium dahliae inoculation by transcriptome sequencing. | verticillium wilt is one of the main diseases in cotton (gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con-trolled effectively. at present, the molecular mechanism that confers resistance to this disease is unclear. transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. in this study, the transcriptome of a disease-resistant inbred cot-ton line inoculated with verticillium da ... | 2015 | 26535625 |
microrna and mrna expression profiling analysis revealed the regulation of plant height in gossypium hirsutum. | dwarf cottons are more resistant to damage from wind and rain and associated with stable, increased yields, and also desirable source for breeding the machine harvest varieties. in an effort to uncover the transcripts and mirna networks involved in plant height, the transcriptome and small rna sequencing were performed based on dwarf mutant ari1327 (a1), tall-culm mutant ari3697 (a3) and wild type ari971 (a9) in gossypium hirsutum. | 2015 | 26517985 |
a bil population derived from g. hirsutum and g. barbadense provides a resource for cotton genetics and breeding. | to provide a resource for cotton genetics and breeding, an interspecific hybridization between gossypium hirsutum cv. emian22 and g. barbadense acc. 3-79 was made. a population of 54 bils (backcross inbred lines, bc1f8) was developed with the aim of transferring g. barbadense genes into g. hirsutum in order to genetically analyze these genes' function in a g. hirsutum background and create new germplasms for breeding. preliminary investigation of the morphological traits showed that the bils had ... | 2015 | 26517274 |
molecular cloning and expression analysis of five ghraxs in upland cotton (gossypium hirsutum l.). | the formation of axillary meristems in leaf axils is a prerequisite for the development of lateral shoots, which largely contribute to plant architecture. several transcription factor-encoding genes, including cuc3, rax, las, lof1, and rox, have been cloned by screening for axillary meristem mutants in arabidopsis thaliana. these genes will facilitate our understanding of the mechanisms underlying axillary meristem development. in this study, we report the cloning of five genes from cotton (goss ... | 2015 | 26505359 |
characterization and development of chloroplast microsatellite markers for gossypium hirsutum, and cross-species amplification in other gossypium species. | cotton is an important economic crop worldwide; its fiber, commonly known as cotton lint, is the main natural source for the textile industry. sixty chloroplast microsatellites were identified and characterized from the complete sequence of the gossypium hirsutum chloroplast genome using a bioinformatic approach. twenty chloroplast microsatellite loci were polymorphic in the 66 gossypium germplasm accessions. a total of 85 alleles were detected, with allele numbers varying from 2-7 per locus. po ... | 2015 | 26505340 |
ghnac12, a neutral candidate gene, leads to early aging in cotton (gossypium hirsutum l). | nac (nam, ataf, and cuc) is one of the largest transcription factor families in plants, and its members play various roles in plant growth, development, and the response to biotic and abiotic stresses. currently, 77 nac genes have been reported in cotton (gossypium hirsutum l.). and ghnac12 showed up-regulation during leaf senescence, but its role in this process is poorly understood. in the present study, a preliminary function analysis of ghnac12 was performed during leaf senescence. qrt-pcr a ... | 2016 | 26496006 |
leafy cotyledon1-casein kinase i-tcp15-phytochrome interacting factor4 network regulates somatic embryogenesis by regulating auxin homeostasis. | somatic embryogenesis (se) is an efficient tool for the propagation of plant species and also, a useful model for studying the regulatory networks in embryo development. however, the regulatory networks underlying the transition from nonembryogenic callus to somatic embryos during se remain poorly understood. here, we describe an upland cotton (gossypium hirsutum) casein kinase i gene, ghcki, which is a unique key regulatory factor that strongly affects se. overexpressing ghcki halted the format ... | 2015 | 26491146 |
sequence composition of bac clones and ssr markers mapped to upland cotton chromosomes 11 and 21 targeting resistance to soil-borne pathogens. | genetic and physical framework mapping in cotton (gossypium spp.) were used to discover putative gene sequences involved in resistance to common soil-borne pathogens. chromosome (chr) 11 and its homoeologous chr 21 of upland cotton (g. hirsutum) are foci for discovery of resistance (r) or pathogen-induced r (pr) genes underlying qtls involved in response to root-knot nematode (meloidogyne incognita), reniform nematode (rotylenchulus reniformis), fusarium wilt (fusarium oxysporum f.sp. vasinfectu ... | 2015 | 26483808 |
overexpression of ghwrky27a reduces tolerance to drought stress and resistance to rhizoctonia solani infection in transgenic nicotiana benthamiana. | wrky proteins constitute transcriptional regulators involved in various biological processes, especially in coping with diverse biotic and abiotic stresses. however, in contrast to other well-characterized wrky groups, the functions of group iii wrky transcription factors are poorly understood in the economically important crop cotton (gossypium hirsutum). in this study, a group iii wrky gene from cotton, ghwrky27a, was isolated and characterized. our data indicated that ghwrky27a localized to t ... | 2015 | 26483697 |
cotton square morphology offers new insights into host plant resistance to cotton fleahopper (hemiptera: miridae) in upland cotton. | cotton fleahopper, pseudatomoscelis seriatus (reuter) (hemiptera: miridae), is a piercing-sucking pest of cotton (gossypium hirsutum l.) that feeds preferentially on developing flower buds, called squares. heavy infestations cause yield reductions that result from abscission of squares damaged by the cotton fleahopper feeding. antixenosis, or nonpreference, has been reported as a mechanism of host plant resistance in cotton to cotton fleahopper. square structure, particularly the placement of th ... | 2016 | 26475922 |
evaluating pilose, a cultigen of gossypium hirsutum, as a source of resistance to cotton fleahopper (hemiptera: miridae). | cotton fleahopper (pseudatomoscelis seriatus reuter) (hemiptera: miridae) is a piercing-sucking insect that has emerged as a major pest of cotton (gossypium hirsutum l.) in texas. cotton fleahoppers feed on floral buds, commonly referred to as squares, causing damage and abscission, and subsequent yield loss. previous studies indicate that plant resistance to cotton fleahopper is present in upland cotton, but the mechanism of resistance remains undetermined. in this study, pilose, a cultigen of ... | 2015 | 26470352 |
relationship between piercing-sucking insect control and internal lint and seed rot in southeastern cotton (gossypium hirsutum l.). | in 1999, crop consultants scouting for stink bugs (hemiptera spp.) in south carolina discovered a formerly unobserved seed rot of cotton that caused yield losses ranging from 10 to 15% in certain fields. the disease has subsequently been reported in fields throughout the southeastern cotton belt. externally, diseased bolls appeared undamaged; internally, green fruit contain pink to dark brown, damp, deformed lint, and necrotic seeds. in greenhouse experiments, we demonstrated transmission of the ... | 2015 | 26470293 |
fall armyworm (lepidoptera: noctuidae) development, survivorship, and damage on cotton plants expressing insecticidal plant-incorporated protectants. | cotton, gossypium hirsutum (l.), plants expressing insecticidal crystal (cry) proteins of bacillus thuringiensis (bt) berliner are planted on significant acreage across the southern region of the united states. fall armyworm, spodoptera frugiperda (j. e. smith), can be a significant cotton pest in some years, but this species has not been a primary target of bt cotton technologies. the objective of this study was to quantify fall armyworm larval survivorship and fruiting form injury on transgeni ... | 2015 | 26470233 |
dirigent proteins from cotton (gossypium sp.) for the atropselective synthesis of gossypol. | gossypol is a defense compound in cotton plants for protection against pests and pathogens. gossypol biosynthesis involves the oxidative coupling of hemigossypol and results in two atropisomers owing to hindered rotation around the central binaphthyl bond. (+)-gossypol predominates in vivo, thus suggesting stereochemically controlled biosynthesis. the aim was to identify the factors mediating (+)-gossypol formation in cotton and to investigate their potential for asymmetric biaryl synthesis. a d ... | 2015 | 26460165 |
high genetic homogeneity points to a single introduction event responsible for invasion of cotton leaf curl multan virus and its associated betasatellite into china. | cotton leaf curl multan virus (clcumuv) is a whitefly transmitted geminivirus (wtg) endemic to the india subcontinent and is notorious as a causal agent of cotton leaf curl disease (clcud), a major constraint to cotton production in south asia. we found clcumuv infecting hibiscus rosa-sinensis in guangzhou, china in 2006. the spread and evolution of the invading clcumuv were monitored in the following nine years. | 2015 | 26445958 |
in silico study for diversing the molecular pathway of pigment formation: an alternative to manual coloring in cotton fibers. | diversity of colors in flowers and fruits is largely due to anthocyanin pigments. the flavonoid/anthocyanin pathway has been most extensively studied. dihydroflavonol 4-reductase (dfr) is a vital enzyme of the flavonoid pathway which displays major impact on the formation of anthocyanins, flavan 3-ols and flavonols. the substrate specificity of the dfr was found to play a crucial role in determination of type of anthocyanidins. altering the flavonoid/anthocyanin pathway through genetic engineeri ... | 2015 | 26442064 |
photosynthetic characteristics of the subtending leaf of cotton boll at different fruiting branch nodes and their relationships with lint yield and fiber quality. | to investigate photosynthetic characteristics of the subtending leaf at the 2-3rd and 10-11th fruiting branch (fbn, fb2-3, and fb10-11), and their relationship with cotton yield and quality, field experiments were conducted using two cotton cultivars, kemian 1 and sumian 15. the results showed that with fbn increasing, chlorophyll (chl) components, pn and non-photochemical quenching (npq) in the subtending leaf significantly declined, while soluble sugar, amino acid and their ratio (c ss/c aa) a ... | 2015 | 26442060 |
identification of early salt stress responsive proteins in seedling roots of upland cotton (gossypium hirsutum l.) employing itraq-based proteomic technique. | soil salinity is a major abiotic stress that limits plant growth and agricultural productivity. upland cotton (gossypium hirsutum l.) is highly tolerant to salinity; however, large-scale proteomic data of cotton in response to salt stress are still scant. here, an isobaric tag for relative and absolute quantitation (itraq)-based proteomic technique was employed to identify the early differentially expressed proteins (deps) from salt-treated cotton roots. one hundred and twenty-eight deps were id ... | 2015 | 26442045 |
cadmium (cd) localization in tissues of cotton (gossypium hirsutum l.), and its phytoremediation potential for cd-contaminated soils. | phytoremediation using economically valuable, large biomass, non-edible plants is a promising method for metal-contaminated soils. this study investigated cotton's tolerance for cd and remediation potential through analyzing cd bioaccumulation and localization in plant organs under different soil cd levels. results showed cotton presents good tolerance when soil cd concentration ≤20.26 mg kg(-1). cotton had good cd accumulation ability under low soil cd levels (<1.26 mg kg(-1)), with a tf value ... | 2015 | 26419249 |
ghwrky25, a group i wrky gene from cotton, confers differential tolerance to abiotic and biotic stresses in transgenic nicotiana benthamiana. | wrky transcription factors are involved in various processes, ranging from plant growth to abiotic and biotic stress responses. group i wrky members have been rarely reported compared with group ii or iii members, particularly in cotton (gossypium hirsutum). in this study, a group i wrky gene, namely, ghwrky25, was cloned from cotton and characterized. expression analysis revealed that ghwrky25 can be induced or deduced by the treatments of abiotic stresses and multiple defense-related signaling ... | 2016 | 26410829 |
genome-scale analysis of the cotton kcs gene family revealed a binary mode of action for gibberellin a regulated fiber growth. | production of β-ketoacyl-coa, which is catalyzed by 3-ketoacyl-coa synthase (kcs), is the first step in very long chain fatty acid (vlcfa) biosynthesis. here we identified 58 kcs genes from gossypium hirsutum, 31 from g. arboreum and 33 from g. raimondii by searching the assembled cotton genomes. the gene family was divided into the plant-specific fae1-type and the more general elo-type. kcs transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or ... | 2016 | 26399709 |
herbicide-resistant cotton (gossypium hirsutum) plants: an alternative way of manual weed removal. | cotton yield has been badly affected by different insects and weed competition. in past application of multiple chemicals is required to manage insects and weed control was achieved by different conventional means, such as hand weeding, crop rotation and polyculture, because no synthetic chemicals were available. the control methods shifted towards high input and target-oriented methods after the discovery of synthetic herbicide in the 1930s. to utilise the transgenic approach, cotton plants exp ... | 2015 | 26383095 |
genome-wide transcriptome analysis of cotton (gossypium hirsutum l.) identifies candidate gene signatures in response to aflatoxin producing fungus aspergillus flavus. | aflatoxins are toxic and potent carcinogenic metabolites produced from the fungi aspergillus flavus and a. parasiticus. aflatoxins can contaminate cottonseed under conducive preharvest and postharvest conditions. united states federal regulations restrict the use of aflatoxin contaminated cottonseed at >20 ppb for animal feed. several strategies have been proposed for controlling aflatoxin contamination, and much success has been achieved by the application of an atoxigenic strain of a. flavus i ... | 2015 | 26366857 |
dose and time dependent efficacy alteration of different defoliants on seed cotton yield. | field experiments were conducted during 2012 and 2013 to determine the effect of select defoliants i.e dropp ultra and ethrel, their optimal dose and suitable time of application on earliness and seed cotton yield in 3 american cotton cultivars. mrc7361bgii (3055.2 kg ha(-1)) and mrc7017bgii (2913.0 kg ha(-1)) resulted in significantly improved seed cotton yield as compared to f1 861 (2346.0 kg ha(-1)). dropp ultra? @ 200 ml ha(-1) revealed highest yield (3018.7 kg ha(-1)) followed by ethrel @ 2 ... | 2015 | 26364466 |
variovorax gossypii sp. nov., isolated from gossypium hirsutum. | a beige-pigmented bacterial strain (jm-310t), isolated from the healthy internal root tissue of 4-week-old cotton (gossypium hirsutum, cultivar 'des-119') in tallassee (macon county), alabama, usa, was studied taxonomically. the isolate produced small rod-shaped cells, which showed a gram-negative staining behaviour. a comparison of the 16s rrna gene sequence of the isolate revealed 99.2, 98.8, 98.7, 98.7, 98.1 and 97.6 % similarity to the 16s rrna gene sequences of the type strains of variovora ... | 2015 | 26341669 |
overexpression of a cotton (gossypium hirsutum) wrky gene, ghwrky34, in arabidopsis enhances salt-tolerance of the transgenic plants. | soil salinity is one of the most serious threats in world agriculture, and often influences cotton growth and development, resulting in a significant loss in cotton crop yield. wrky transcription factors are involved in plant response to high salinity stress, but little is known about the role of wrky transcription factors in cotton so far. in this study, a member (ghwrky34) of cotton wrky family was functionally characterized. this protein containing a wrky domain and a zinc-finger motif belong ... | 2015 | 26332661 |
comparisons of lygus lineolaris (hemiptera: miridae) populations from two distinct geographical regions of mississippi. | the tarnished plant bug, lygus lineolaris (palisot de beauvois) (hemiptera: miridae), is a major pest of cotton (gossypium hirsutum l.) in the state of mississippi. economic data indicate that l. lineolaris is a more serious pest of cotton in the delta region of mississippi than in the hills region; however, little data exist comparing the two populations. two experiments were undertaken to compare l. lineolaris from these two geographically distinct regions. in the first experiment, colonies of ... | 2015 | 26313997 |
influence of crop management and environmental factors on wolf spider assemblages (araneae: lycosidae) in an australian cotton cropping system. | wolf spiders (lycosidae) are the most abundant ground-hunting spiders in the australian cotton (gossypium hirsutum l.) agroecosystems. these spiders have potential in controlling pest bollworms, helicoverpa spp. (lepidoptera: noctuidae) in minimum-tilled fields. a study was carried out during a wet growing season (2011-2012) in narrabri, new south wales, australia, to determine how different crop rotations and tillage affect wolf spider assemblages in cotton fields. spider abundance and species ... | 2015 | 26308820 |
bacillus gossypii sp. nov., isolated from the stem of gossypium hirsutum. | a gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of gossypium hirsutum, was studied to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity comparisons, strain jm-267t was grouped in the genus bacillus, related most closely to the type strains of bacillus simplex and bacillus huizhouensis (both 97.8%), bacillus muralis (97.7%), bacillus butanolivorans and bacillus psychrosaccharolyticus (both 97.3%). 16s rrna gene s ... | 2015 | 26303844 |
a high-density ssr genetic map constructed from a f2 population of gossypium hirsutum and gossypium darwinii. | the cultivated allotetraploid species gossypium hirsutum, accounts for 90% of the world cotton production, has narrow genetic basis that's why its yield, quality or stress resistance breeding is stagnant. it is therefore, essential to explore desirable genes from gossypium darwinii which has enviable traits such as high fiber fineness, drought tolerance, fusarium and verticillium resistance. we used g. darwinii as primary plant materials in this study not only to enrich the genetic diversity of ... | 2015 | 26275937 |
gbexpatr, a species-specific expansin, enhances cotton fibre elongation through cell wall restructuring. | cotton provides us the most important natural fibre. high fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from gossypium barbadense to gossypium hirsutum is an important breeding strategy. we previously analysed the g. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre-specific α-expansins from g. barbadense, gbexpa2 and gbexpatr. gbexpa2 (from the dt genome) is a classical α-e ... | 2016 | 26269378 |
high resolution consensus mapping of quantitative trait loci for fiber strength, length and micronaire on chromosome 25 of the upland cotton (gossypium hirsutum l.). | cotton (gossypium hirsutum l.) is an important agricultural crop that provides renewable natural fiber resources for the global textile industry. technological developments in the textile industry and improvements in human living standards have increased the requirement for supplies and better quality cotton. upland cotton 0-153 is an elite cultivar harboring strong fiber strength genes. to conduct quantitative trait locus (qtl) mapping for fiber quality in 0-153, we developed a population of 19 ... | 2015 | 26262992 |
transcriptome analysis reveals that distinct metabolic pathways operate in salt-tolerant and salt-sensitive upland cotton varieties subjected to salinity stress. | salinity stress is one of the most devastating abiotic stresses in crop plants. as a moderately salt-tolerant crop, upland cotton (gossypium hirsutum l.) is a major cash crop in saline areas and a suitable model for salt stress tolerance research. in this study, we compared the transcriptome changes between the salt-tolerant upland cotton cultivar zhong 07 and salt-sensitive cultivar zhong g5 in response to nacl treatments. transcriptional regulation, signal transduction and secondary metabolism ... | 2015 | 26259172 |
host-associated differentiation in a highly polyphagous, sexually reproducing insect herbivore. | insect herbivores may undergo genetic divergence on their host plants through host-associated differentiation (had). much of what we know about had involves insect species with narrow host ranges (i.e., specialists) that spend part or all their life cycle inside their hosts, and/or reproduce asexually (e.g., parthenogenetic insects), all of which are thought to facilitate had. however, sexually reproducing polyphagous insects can also exhibit had. few sexually reproducing insects have been teste ... | 2015 | 26257868 |
interspecific associations between cycloneda sanguinea and two aphid species (aphis gossypii and hyadaphis foeniculi) in sole-crop and fennel-cotton intercropping systems. | aphids cause significant damage to crop plants. studies regarding predator-prey relationships in fennel (foeniculum vulgare mill.) and cotton (gossypium hirsutum l.) crops are important for understanding essential ecological interactions in the context of intercropping and for establishing pest management programs for aphids. this study evaluated the association among hyadaphis foeniculi (passerini) (hemiptera: aphididae), aphis gossypii glover (hemiptera: aphididae) and cycloneda sanguinea (l.) ... | 2015 | 26241862 |
construction of bac contig maps of homoeologous chromosomes a12 and d12 of gossypium hirsutum l. acc. tm-1. | the gossypium hirsutum homoeologous chromosome 12 encodes important genes that contribute to fiber fuzz, lethality, gland development and male sterility. in this study a physical map of the cotton tm-1 chromosome 12 was constructed. a number of large-insert cotton genome libraries are available, and genome-wide physical mapping using large insert segments combined with bacterial cloning is a thriving area of genome research. however, sequencing of the cotton genome is difficult due to sequence r ... | 2015 | 26221184 |
the mitochondrial malate dehydrogenase 1 gene ghmmdh1 is involved in plant and root growth under phosphorus deficiency conditions in cotton. | cotton, an important commercial crop, is cultivated for its natural fibers, and requires an adequate supply of soil nutrients, including phosphorus, for its growth. soil phosporus exists primarily in insoluble forms. we isolated a mitochondrial malate dehydrogenase (mdh) gene, designated as ghmmdh1, from gossypium hirsutum l. to assess its effect in enhancing p availability and absorption. an enzyme kinetic assay showed that the recombinant ghmmdh1 possesses the capacity to catalyze the intercon ... | 2015 | 26179843 |
effect of the postfeeding interval on olfactory responses of thrips to herbivore-induced cotton plants. | we investigated the responses of 3 thrips species, frankliniella schultzei trybom, f. occidentalis pergrande, and thrips tabaci lindeman (thysanoptera: thripidae) to herbivore-damaged and undamaged cotton seedlings (gossypium hirsutum l. [malvales: malvaceae]) at a range of time intervals following damage by adult tetranychus urticae (koch), adult t. ludeni (zacher) (acari: tetranychidae) or helicoverpa armigera (hübner) (lepidoptera: noctuidae) larvae in olfactometer assays. the intensity/frequ ... | 2016 | 26172119 |
dynamic transcriptome analysis and volatile profiling of gossypium hirsutum in response to the cotton bollworm helicoverpa armigera. | in response to insect herbivory, plants emit elevated levels of volatile organic compounds for direct and indirect resistance. however, little is known about the molecular and genomic basis of defense response that insect herbivory trigger in cotton plants and how defense mechanisms are orchestrated in the context of other biological processes. here we monitored the transcriptome changes and volatile characteristics of cotton plants in response to cotton bollworm (cbw; helicoverpa armigera) larv ... | 2015 | 26148847 |
promoting flowering, lateral shoot outgrowth, leaf development, and flower abscission in tobacco plants overexpressing cotton flowering locus t (ft)-like gene ghft1. | flowering locus t (ft) encodes a mobile signal protein, recognized as major component of florigen, which has a central position in regulating flowering, and also plays important roles in various physiological aspects. a mode is recently emerging for the balance of indeterminate and determinate growth, which is controlled by the ratio of ft-like and terminal flower 1 (tfl1)-like gene activities, and has a strong influence on the floral transition and plant architecture. orthologs of ghft1 was pre ... | 2015 | 26136765 |
identification of mirnas and their targets in cotton inoculated with verticillium dahliae by high-throughput sequencing and degradome analysis. | micrornas (mirnas) are a group of endogenous small non-coding rnas that play important roles in plant growth, development, and stress response processes. verticillium wilt is a vascular disease in plants mainly caused by verticillium dahliae kleb., the soil-borne fungal pathogen. however, the role of mirnas in the regulation of verticillium defense responses is mostly unknown. this study aimed to identify new mirnas and their potential targets that are involved in the regulation of verticillium ... | 2015 | 26133244 |
predawn respiration rates during flowering are highly predictive of yield response in gossypium hirsutum when yield variability is water-induced. | respiratory carbon evolution by leaves under abiotic stress is implicated as a major limitation to crop productivity; however, respiration rates of fully expanded leaves are positively associated with plant growth rates. given the substantial sensitivity of plant growth to drought, it was hypothesized that predawn respiration rates (rpd) would be (1) more sensitive to drought than photosynthetic processes and (2) highly predictive of water-induced yield variability in gossypium hirsutum. two stu ... | 2015 | 26125121 |
development of molecular markers for genetic male sterility in gossypium hirsutum. | genetic male sterility (gms) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. sequence analysis on ms5 and ms6 loci in gossypium hirsutum was conducted to reveal genomic variation at these two loci between gms and wild-type g. hirsutum inbred lines, and sequence polymorphism linked to ms5 on a12 and ms6 on d12 was revealed. a haplotype marker set that consisted of four snps targeting both ms5 and ms6 gene r ... | 2017 | 26074724 |
the complexity of posttranscriptional small rna regulatory networks revealed by in silico analysis of gossypium arboreum l. leaf, flower and boll small regulatory rnas. | micrornas (mirnas) and secondary small interfering rnas (principally phased sirnas or trans-acting sirnas) are two distinct subfamilies of small rnas (srnas) that are emerging as key regulators of posttranscriptional gene expression in plants. both mirnas and secondary-sirnas (sec-sirnas) are processed from longer rna precursors by dicer-like proteins (dcls). gossypium arboreum l., also known as tree cotton or asian cotton, is a diploid, possibly ancestral relative of tetraploid gossypium hirsut ... | 2015 | 26070200 |
a new synthetic amphiploid (aaddaa) between gossypium hirsutum and g. arboreum lays the foundation for transferring resistances to verticillium and drought. | gossypium arboreum, a cultivated cotton species (2n = 26, aa) native to asia, possesses invaluable characteristics unavailable in the tetraploid cultivated cotton gene pool, such as resistance to pests and diseases and tolerance to abiotic stresses. however, it is quite difficult to transfer favorable traits into upland cotton through conventional methods due to the cross-incompatibility of g. hirsutum (2n = 52, aadd) and g. arboreum. here, we improved an embryo rescue technique to overcome the ... | 2015 | 26061996 |
tillage impact on herbicide loss by surface runoff and lateral subsurface flow. | there is worldwide interest in conservation tillage practices because they can reduce surface runoff, and agrichemical and sediment losses from farm fields. since these practices typically increase infiltration, their use may increase subsurface transport of water-soluble contaminants. thus, to assess long-term environmental benefits of conservation tillage data may be needed that quantify both surface and subsurface contaminant fluxes. this study focused on the herbicide fluometuron (n,n-dimeth ... | 2015 | 26057540 |
interference between redroot pigweed (amaranthus retroflexus l.) and cotton (gossypium hirsutum l.): growth analysis. | redroot pigweed is one of the injurious agricultural weeds on a worldwide basis. understanding of its interference impact in crop field will provide useful information for weed control programs. the effects of redroot pigweed on cotton at densities of 0, 0.125, 0.25, 0.5, 1, 2, 4, and 8 plants m(-1) of row were evaluated in field experiments conducted in 2013 and 2014 at institute of cotton research, caas in china. redroot pigweed remained taller and thicker than cotton and heavily shaded cotton ... | 2015 | 26057386 |
itraq-facilitated proteomic profiling of anthers from a photosensitive male sterile mutant and wild-type cotton (gossypium hirsutum l.). | male sterility is a common phenomenon in flowering plants, and it has been successfully developed in several crops by taking advantage of heterosis. cotton (gossypium hirsutum l.) is an important economic crop, used mainly for the production of textile fiber. using a space mutation breeding technique, a novel photosensitive genetic male sterile mutant ccri9106 was isolated from the wild-type upland cotton cultivar ccri040029. to use ccri9106 in cotton hybrid breeding, it is of great importance t ... | 2015 | 26047712 |
genetic fine mapping and candidate gene analysis of the gossypium hirsutum ligon lintless-1 (li1) mutant on chromosome 22(d). | ligon lintless-1 (li1) is a gossypium hirsutum mutant that is controlled by a dominant gene that arrests the development of cotton fiber after anthesis. two f2 mapping populations were developed from mutant (li1 × h7124) f1 plants in 2012 and 2013; each was composed of 142 and 1024 plants, respectively. using these populations, li1 was mapped to a 0.3-cm region in which nine single-strand conformation polymorphism markers co-segregated with the li1 locus. in the published g. raimondii genome, th ... | 2015 | 26037218 |
mapping-by-sequencing of ligon-lintless-1 (li 1 ) reveals a cluster of neighboring genes with correlated expression in developing fibers of upland cotton (gossypium hirsutum l.). | mapping-by-sequencing and snp marker analysis were used to fine map the ligon-lintless-1 ( li 1 ) short fiber mutation in tetraploid cotton to a 255-kb region that contains 16 annotated proteins. the ligon-lintless-1 (li 1 ) mutant of cotton (gossypium hirsutum l.) has been studied as a model for cotton fiber development since its identification in 1929; however, the causative mutation has not been identified yet. here we report the fine genetic mapping of the mutation to a 255-kb region that co ... | 2015 | 26021293 |
inheritance and qtl mapping of leaf nutrient concentration in a cotton inter-specific derived ril population. | developing and deploying cotton cultivars with high nutrient uptake, use efficiency and tolerance to nutrient related soil stresses is desirable to assist sustainable soil management. genetic variation, heritability, selection response and quantitative trait loci (qtls) were investigated for five macronutrients (p, k, ca, mg, s) and five micronutrients (fe, mn, b, zn, and cu) in a recombinant inbred line (ril) population from an inter-specific cross between gossypium hirsutum cv. guazuncho 2, an ... | 2015 | 26020945 |
a novel nap member ghnap is involved in leaf senescence in gossypium hirsutum. | premature leaf senescence has a negative influence on the yield and quality of cotton, and several genes have been found to regulate leaf senescence. howeer, many underlying transcription factors are yet to be identified. in this study, a nap-like transcription factor (ghnap) was isolated from gossypium hirsutum. ghnap has the typical nac structure and a conserved novel subdomain in its divergent transcription activation region (tar). ghnap was demonstrated to be a nuclear protein, and it showed ... | 2015 | 25991739 |
novosphingobium gossypii sp. nov., isolated from gossypium hirsutum. | a gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain jm-1396(t)) producing a yellow pigment, was isolated from the healthy internal stem tissue of post-harvest cotton (gossypium hirsutum, cultivar 'des-119') grown at the plant breeding unit at the e.v. smith research center in tallassee (macon county), al, usa. 16s rrna gene sequence analysis of strain jm-1396(t) showed high sequence similarity values to the type strains of novosphingobium mathurense, novosphingobium panipatens ... | 2015 | 25985829 |
cloning and expression analysis of hsp70 gene from mangrove plant kandelia obovata under cold stress. | heat shock protein 70 (hsp70), the primary member of the hsps that play various stress-protective roles in plants. in this study, a hsp70 gene of kandelia obovata (kohsp70) was cloned by rapid amplification of cdna ends (race). the full-length of kohsp70 was 2255 bp, consisting of a 5'-terminal untranslated region (utr) of 118 bp, a 3'-terminal utr of 178 bp, and an open reading frame (orf) of 1959 bp. the orf (kohsp70) was predicted to encode a polypeptide of 652 amino acids with a theoretical ... | 2015 | 25980488 |
non-destructive high-throughput dna extraction and genotyping methods for cotton seeds and seedlings. | extensive use of targeted pcr-based genotyping is precluded for many plant research laboratories by the cost and time required for dna extraction. using cotton (gossypium hirsutum) as a model for plants with medium-sized seeds, we report here manual procedures for inexpensive non-destructive high-throughput extraction of dna suitable for pcr-based genotyping of large numbers of individual seeds and seedlings. by sampling only small amounts of cotyledon tissue of ungerminated seed or young seedli ... | 2015 | 25967902 |
molecular cloning and expression analysis of ghlof genes in upland cotton (gossypium hirsutum l.). | shoot branching, i.e., the timing and position of shoot growth, determines to a large extend the pattern of plant architecture, and is the result of the integration of a plant's genetic background and environmental cues. many genes that are involved in the formation and outgrowth of axillary buds have been cloned, but the exact mechanism is still unclear. branching pattern is an important agronomic trait in many crops, including cotton. in the present study, we cloned four genes from cotton, and ... | 2015 | 25966231 |
development of gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the g. anomalum and g. hirsutum genomes. | we reported the first development of gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. to continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as gossypium anomalum to cultivated allotetraploid cotton species. however, interspecific reproductive barriers limit gene transfer between species. in a previ ... | 2015 | 25957115 |
genetic mapping and comparative expression analysis of transcription factors in cotton. | transcription factors (tfs) play an important role in the regulation of plant growth and development. the study of the structure and function of tfs represents a research frontier in plant molecular biology. the findings of these studies will provide significant information regarding genetic improvement traits in crops. currently, a large number of tfs have been cloned, and their function has been verified. however, relatively few studies that genetically map tfs in cotton are available. to gene ... | 2015 | 25946129 |
overexpression of 3-deoxy-7-phosphoheptulonate synthase gene from gossypium hirsutum enhances arabidopsis resistance to verticillium wilt. | expression of dhs1 in cotton is induced upon infection by verticillium dahliae , and overexpression of ghdhs1 endows transgenic arabidopsis plants excellent verticillium resistance. verticillium wilt is caused by a soil-borne fungus verticillium dahliae. resistance in most cotton cultivars is either scarce or unavailable, making verticillium wilt a major obstacle in cotton production. here, we identified a 3-deoxy-7-phosphoheptulonate synthase (dhs, ec 4.1.2.15) gene from gossypium hirsutum, nam ... | 2015 | 25929795 |