Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain lactobacillus johnsonii ncc933 and vaginal strain lactobacillus gasseri ks120.1 against enteric, uropathogenic and vaginosis-associated pathogens. | the mechanism underlying the killing activity of lactobacillus strains against bacterial pathogens appears to be multifactorial. here, we investigate the respective contributions of hydrogen peroxide and lactic acid in killing bacterial pathogens associated with the human vagina, urinary tract or intestine by two hydrogen peroxide-producing strains. in co-culture, the human intestinal strain lactobacillus johnsonii ncc933 and human vaginal strain lactobacillus gasseri ks120.1 strains killed ente ... | 2010 | 20082639 |
| metronidazole is still the drug of choice for treatment of anaerobic infections. | metronidazole has been used for the treatment of infections for >45 years and is still successfully used for the treatment of trichomoniasis, amoebiasis, and giardiasis. anaerobic bacterial infections caused by bacteroides species, fusobacteria, and clostridia respond favorably to metronidazole therapy. good clinical results in the treatment of vaginosis due to gardnerella vaginalis have also been reported. rates of resistance to metronidazole are still generally low; however, several studies ha ... | 2010 | 20067388 |
| high vaginal concentrations of atopobium vaginae and gardnerella vaginalis in women undergoing preterm labor. | to estimate the relationship between vaginal quantification of the main microorganisms related with bacterial vaginosis and the risk of preterm delivery among women with preterm labor. | 2010 | 20027045 |
| glycerol monolaurate inhibits candida and gardnerella vaginalis in vitro and in vivo but not lactobacillus. | we investigated the effects of glycerol monolaurate (gml) on lactobacillus, candida, and gardnerella vaginalis human vaginal microflora. our previous work demonstrated that 6 months of gml treatment vaginally does not alter lactobacillus counts in monkeys. candida and g. vaginalis are commonly associated with vaginal infections in women, many becoming chronic or recurrent. in vitro growth inhibition studies determined the effects of gml (0 to 500 microg/ml) against multiple candida species and g ... | 2010 | 20008774 |
| comparison of main lactobacillus species between healthy women and women with bacterial vaginosis. | the normal microbial flora of the vagina plays an important role in preventing genital and urinary tract infections in women. thus an accurate understanding of the composition and ecology of the ecosystem is important to understanding the etiology of these diseases. this study aimed to compare the characteristics of main lactobacillus species between healthy women and women with bacterial vaginosis (bv) by quantitative culture and pcr methods. main lactobacillus species include l. crispatus, l. ... | 2009 | 19951608 |
| in vitro inhibitory activity of human vaginal lactobacilli against pathogenic bacteria associated with bacterial vaginosis in kenyan women. | lactobacilli have been shown to inhibit in vitro growth of many pathogens and have been used as probiotics to treat a broad range of gastrointestinal and/or vaginal disorders. we sought to determine the in vitro inhibitory potential of lactobacilli of vaginal origin to some bacteria associated with bacterial vaginosis (bv), to characterize the inhibitory substances produced by these lactobacilli and to assess h2o2 production. vaginal specimens were obtained by swabbing the lateral vaginal walls ... | 2010 | 19925874 |
| analysis of adherence, biofilm formation and cytotoxicity suggests a greater virulence potential of gardnerella vaginalis relative to other bacterial-vaginosis-associated anaerobes. | worldwide, bacterial vaginosis (bv) is the most common vaginal disorder in women of childbearing age. bv is characterized by a dramatic shift in the vaginal microflora, involving a relative decrease in lactobacilli, and a proliferation of anaerobes. in most cases of bv, the predominant bacterial species found is gardnerella vaginalis. however, pure cultures of g. vaginalis do not always result in bv, and asymptomatic women are sometimes colonized with low numbers of g. vaginalis. thus, there is ... | 2010 | 19910411 |
| bacterial vaginosis: a cause of infertility? | bacterial vaginosis (bv) is a common disorder of the genital tract in women characterized by an alteration of the normal acidic lactobacilli-predominant vaginal ecosystem to a vaginal environment dominated by gardnerella vaginalis, mycoplasma species and anaerobes, with an increase in ph. the present study evaluated whether bv is associated with reproductive complications in women. bv was screened with a gram stain of vaginal smear and interpretation was done using the nugent score. wet mount an ... | 2009 | 19833694 |
| treatment and management of bacterial vaginosis in pregnancy: current and future perspectives. | bacterial vaginosis accounts for the majority of cases of vaginal discharge and has been consistently linked to an increased risk of preterm delivery. bacterial vaginosis is characterized by the reduced number or absence of hydrogen peroxide-producing lactobacillus spp., which promotes the overgrowth of anaerobic bacteria, including gardnerella vaginalis, mycoplasma hominis, bacteroides spp., and mobiluncus spp. black race, higher-risk sexual activity, frequent vaginal douching and the substanti ... | 2006 | 19803898 |
| inflammatory events as detected in cervical smears and squamous intraepithelial lesions. | the dutch cytological coding system, kopac, enables to code for eight inflammatory events, that is koilocytosis (related to human papillomavirus (hpv)), trichomonas, dysbacteriosis [related to bacterial vaginosis (bv)], candida, gardnerella, actinomyces, chlamydia, and non-specific inflammation (leucocytosis). this study presents an analysis of 1,008,879 smears. of each smear, the age of the woman and the reason for smear taking (screening or indication) was available. the cytoscores (per mille) ... | 2010 | 19795485 |
| multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres. | bacterial vaginosis (bv) is a recurrent condition that is associated with a range of negative outcomes, including the acquisition of human immunodeficiency virus and other sexually transmitted diseases, preterm births, and pelvic inflammatory disease. in contrast to the lactobacillus-dominated normal vaginal microbiota, bv is characterized by a lack of lactobacilli and an abundance of anaerobic and gram-negative organisms, including gardnerella vaginalis and atopobium vaginae. to date, the labor ... | 2009 | 19794034 |
| gardnerella infection as distinguished from cervical dysbacteriosis: the advantageous spin-off of cervical screening. | to evaluate cytologic diagnoses of dysbacteriosis and gardnerella infection and to obtain insight into the diagnostic problems of gardnerella. | 2009 | 19697722 |
| changes in genital anatomy and microbiology in girls between age 6 and age 12 years: a longitudinal study. | to study changes in genital anatomy and occurrence of human papillomavirus and gardnerella vaginalis in girls resulting from growth and development. | 2010 | 19643638 |
| colonization of gardnerella vaginalis in semen of infertile men: prevalence, influence on sperm characteristics, relationship with leukocyte concentration and clinical significance. | to determine the prevalence of gardnerella vaginalis in semen from infertile men and their negative effects on semen quality. | 2009 | 19602896 |
| in vitro activity of secnidazole against atopobium vaginae, an anaerobic pathogen involved in bacterial vaginosis. | bacterial vaginosis is a polymicrobial syndrome. the most important marker for bacterial vaginosis is the presence of gardnerella vaginalis and atopobium vaginae. in this study, the in vitro susceptibilities to metronidazole and secnidazole of 16 strains of a. vaginae were tested with the agar dilution method. we observed an mic range for metronidazole of 4-64 mg/l (mic(50), 8 mg/l; mic(90), 32 mg/l) and an mic range for secnidazole of 4-128 mg/l (mic(50), 16 mg/l; mic(90), 64 mg/l). according t ... | 2010 | 19548924 |
| vertebral osteomyelitis and discitis due to gardnerella vaginalis. | gardnerella vaginalis is a facultative anaerobic gram-variable pleomorphic rod that forms part of the normal vaginal flora. it is most commonly associated with infection of the genital tract in women, but recognition of extravaginal g. vaginalis infection is becoming more frequent. we describe an unusual case of g. vaginalis vertebral osteomyelitis and discitis in a 38-year-old woman with no apparent predisposing factors. | 2009 | 19541786 |
| comparison of oral and vaginal metronidazole for treatment of bacterial vaginosis in pregnancy: impact on fastidious bacteria. | bacterial vaginosis (bv) is a common condition that is associated with preterm birth and acquisition of complex communities of vaginal bacteria that include several fastidious species. treatment of bv in pregnancy has mixed effects on the risk of preterm delivery, which some hypothesize is due to variable antibiotic efficacy for the fastidious bacteria. both oral and intravaginal metronidazole can be used to treat bacterial vaginosis in pregnancy, but little is known about the impact of differen ... | 2009 | 19515236 |
| microflora of the penile skin-lined neovagina of transsexual women. | the microflora of the penile skin-lined neovagina in male-to-female transsexuals is a recently created microbial niche which thus far has been characterized only to a very limited extent. yet the knowledge of this microflora can be considered as essential to the follow-up of transsexual women. the primary objective of this study was to map the neo-vaginal microflora in a group of 50 transsexual women for whom a neovagina was constructed by means of the inverted penile skin flap technique. second ... | 2009 | 19457233 |
| bacterial vaginosis: an overview for 2009. | bacterial vaginosis has been appreciated as a unique clinical entity for well over 50 years. its essential manifestations are well established: a loss of the normal bacterial population of the vagina and their replacement by other species. investigations into this condition have led to a better understanding of its prevalence and epidemiology. microbiologic and biochemical studies have exposed the remarkably complex pathophysiologic events that occur with bacterial vaginosis. several major morbi ... | 2009 | 19399292 |
| oleate lipase activity in gardnerella vaginalis and reconsideration of existing biotype schemes. | gardnerella vaginalis is a facultative gram positive organism that requires subculture every 1-2 days to maintain viability. it has been linked with bacterial vaginosis (bv), a syndrome that has been associated with increased risk for preterm delivery, pelvic inflammatory disease and hiv acquisition. about 10% of the g. vaginalis isolates have been reported to produce sialidase, but there have not been any studies relating sialidase production and biotype. sialidase activity is dramatically incr ... | 2009 | 19386125 |
| antibody-based detection and inhibition of vaginolysin, the gardnerella vaginalis cytolysin. | bacterial vaginosis (bv) is the most common vaginal infection worldwide and is associated with significant adverse sequelae. we have recently characterized vaginolysin (vly), the human-specific cytotoxin produced by gardnerella vaginalis and believed to play a critical role in the pathogenesis of bv and its associated morbidities. we hypothesize that novel antibody-based strategies may be useful for detection of vly and for inhibition of its toxic effects on human cells. using purified toxin as ... | 2009 | 19370149 |
| simultaneous identification of 14 genital microorganisms in urine by use of a multiplex pcr-based reverse line blot assay. | the aim of this study was to develop and evaluate a sensitive method for the simultaneous identification of 14 urogenital potential pathogens. a multiplex pcr-based reverse line blot (mpcr/rlb) assay was developed to detect 14 urogenital pathogens or putative pathogens, namely trichomonas vaginalis, streptococcus pneumoniae, neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma parvum, u. urealyticum, gardnerella vaginalis, haemophilus influenzae, herpes simplex virus type 1 (hsv1) and hsv2, ... | 2009 | 19357202 |
| retinal vasculitis associated with asymptomatic gardnerella vaginalis infection: a new clinical entity. | to report on 3 cases of retinal vasculitis associated with asymptomatic gardnerella vaginalis (gv) infection. | 2009 | 19294572 |
| does progesterone inhibit bacteria-stimulated interleukin-8 production by lower genital tract epithelial cells? | progesterone (p(4)) has been clinically shown to prevent the recurrence of preterm birth. the mechanism(s) of action is unclear, but may involve modulation of the immunologic inflammatory response of the lower genital tract. we evaluated the effects of p(4) on interleukin-8 (il-8) production by vaginal and cervical epithelial cells stimulated with bacterial species that are commonly associated with preterm birth. | 2009 | 19290856 |
| [septic shock caused by gardnerella vaginalis and peptostreptococcus species after cesarean section]. | a 41-year old multisectio patient got a life-threatening postoperative infection with gardnerella vaginalis and peptostreptococcus spp. perioperative treatment with cefuroxime and metronidazol is recommended. | 2009 | 19284925 |
| the human vaginal bacterial biota and bacterial vaginosis. | the bacterial biota of the human vagina can have a profound impact on the health of women and their neonates. changes in the vaginal microbiota have been associated with several adverse health outcomes including premature birth, pelvic inflammatory disease, and acquisition of hiv infection. cultivation-independent molecular methods have provided new insights regarding bacterial diversity in this important niche, particularly in women with the common condition bacterial vaginosis (bv). pcr method ... | 2009 | 19282975 |
| effect of sulfasalazine on basal and bacteria-stimulated interleukin-8 production by endocervical epithelial cells. | sulfasalazine (sasp) inhibits lipopolysaccharide-induced nuclear-factor kappa b activation and interleukin-8 (il-8) production by cultured explants of placenta, amnion and choriodecidua. bacteria-induced il-8 production in the cervix is a potential mechanism for premature cervical ripening that may lead to preterm birth. our objective was to determine if sasp inhibits il-8 production by endocervical cells stimulated with bacterial pathogens associated with preterm birth. | 2009 | 19239421 |
| validation of an immunologic diagnostic kit for infectious vaginitis by trichomonas vaginalis, candida spp., and gardnerella vaginalis. | fempure is a kit for the rapid diagnosis of vaginitis by trichomonas vaginalis, candida spp., and gardnerella vaginalis, based on aggregation of latex particles joined to specific antibodies. the validation of the method involved the parameters specificity, detection limit, robustness, clinical sensitivity, and clinical specificity. also, samples analyzed in parallel by the validated test and other recognized tests conducted by external laboratory were included. the method was specific for the 3 ... | 2009 | 19216938 |
| gene polymorphisms of toll-like and related recognition receptors in relation to the vaginal carriage of gardnerella vaginalis and atopobium vaginae. | host genetic factors have previously been found to act as determinants of differential susceptibility to major infectious diseases. it is less clear whether such polymorphisms may also impose on pathogen recognition in mucosal overgrowth conditions such as bacterial vaginosis, an anaerobic overgrowth condition characterised by the presence of a vaginal biofilm consisting of the gram-positive anaerobes gardnerella vaginalis and atopobium vaginae. we selected 34 single nucleotide polymorphisms per ... | 2009 | 19200604 |
| adhesion of human probiotic lactobacillus rhamnosus to cervical and vaginal cells and interaction with vaginosis-associated pathogens. | the ability of a probiotic lactobacillus rhamnosus strain (lcr35) to adhere to cervical and vaginal cells and to affect the viability of two main vaginosis-associated pathogens, prevotella bivia, gardnerella vaginalis, as well as candida albicans was investigated. | 2009 | 19190778 |
| cervicovaginal cytological abnormalities in patients with human immunodeficiency virus infection, in relation to disease stage, cd4 cell count and viral load. | the objective of the present study was to assess infections and cytologic abnormalities in cervicovaginal smears from 153 hiv-positive women and 169 hiv-negative followed up at the uftm school of medicine between may 1999 and may 2002. the medical records and cervicovaginal smears were reviewed and the hiv-positive group was classified according to cd4 cell count, hiv viral load, antiretroviral therapy and hiv subgroups (with or without disease; with or without therapy) and compared to hiv-negat ... | 2009 | 19170167 |
| changes in vaginal bacterial concentrations with intravaginal metronidazole therapy for bacterial vaginosis as assessed by quantitative pcr. | several fastidious bacteria have been associated with bacterial vaginosis (bv) using broad-range bacterial pcr methods such as consensus sequence 16s rrna gene pcr, but their role in bv remains poorly defined. we describe changes in vaginal bacterial concentrations following metronidazole therapy for bv. vaginal swabs were collected from women with bv diagnosed using amsel clinical criteria, and vaginal fluid was assessed by gram stain to generate nugent scores. follow-up swabs were collected 1 ... | 2009 | 19144794 |
| clinical evaluation of affirm vp iii in the detection and identification of bacterial vaginosis. | 2008 | 19129080 | |
| bacterial vaginosis presence in sexually active women in tuzla canton area. | the goal of our research was to determine the presence of bacterial vaginosis in sexually active women in tuzla canton area. diagnosis determination for bacterial vaginosis was conducted on the basis of three out of four internationally accepted criteria according to amsel and isolation and identification of gardnerella vaginalis (g. vaginalis) by standard microbiological procedures. bacterial vaginosis was diagnosed in 20,5 % (41/200) women who asked for gynaecologist's help due to their person ... | 2008 | 19125702 |
| accuracy of the clinical diagnosis of vaginitis compared with a dna probe laboratory standard. | to estimate the accuracy of the clinical diagnosis of the three most common causes of acute vulvovaginal symptoms (bacterial vaginosis, candidiasis vaginitis, and trichomoniasis vaginalis) using a traditional, standardized clinical diagnostic protocol compared with a dna probe laboratory standard. | 2009 | 19104364 |
| what's the treatment for bacterial vaginosis? | a 28-weeks-pregnant patient complained of vaginal irritation and a malodorous white discharge that didn't improve after she used an over-the-counter cream indicated for yeast infections. what's a more effective treatment? | 2008 | 19033972 |
| gardnerella vaginalis acute hip arthritis in a renal transplant recipient. | we report the case of an acute hip septic arthritis caused by gardnerella vaginalis in a 48-year-old woman under immunosuppressive therapy for kidney transplantation. after surgical resection of the hip and 6 weeks of combination antibiotic therapy, a total hip prosthesis was successfully implanted with no recurrence after 4 years. | 2009 | 19020054 |
| preterm labor and bacterial vaginosis-associated bacteria among urban women. | bacterial vaginosis (bv) affects millions of women, is extremely prevalent and is frequently chronic. we recognize numerous microbiologic variations among women with bv and this variability may explain the limited effectiveness of metronidazole in curing bv and/or reducing the risk of spontaneous preterm birth (sptb) among bv-positive pregnant women. we assessed the independent role of seven common bv-associated bacteria on the risk of spontaneous preterm birth (sptb) among urban pregnant women. | 2009 | 18999913 |
| utility of reflexive semen cultures for detecting bacterial infections in patients with infertility and leukocytospermia. | this study evaluated the benefit of reflexive semen cultures in peroxidase-positive samples drawn from 17,142 consecutive samples analyzed, in which 4.7% (807/17,142) had leukocytospermia and 12.7% of those (103/807) grew significant bacterial colonies on culture. considering the potential ramifications of the use of samples with bacteriospermia, we conclude that reflexive culture results are cost-effective and prudent. | 2009 | 18980764 |
| longitudinal trajectory of bacterial vaginosis during pregnancy. | this study was undertaken to characterize the course of bacterial vaginosis in pregnancy and to discern the bacterial morphotypes responsible for infection. | 2008 | 18928996 |
| spermicidal activity of the safe natural antimicrobial peptide subtilosin. | bacterial vaginosis (bv), a condition affecting millions of women each year, is primarily caused by the gram-variable organism gardnerella vaginalis. a number of organisms associated with bv cases have been reported to develop multidrug resistance, leading to the need for alternative therapies. previously, we reported the antimicrobial peptide subtilosin has proven antimicrobial activity against g. vaginalis, but not against the tested healthy vaginal microbiota of lactobacilli. after conducting ... | 2008 | 18923673 |
| prevotella bivia as a source of lipopolysaccharide in the vagina. | to compare vaginal lipopolysaccharides (lps) concentrations between patients with and without bacterial vaginosis (bv), to evaluate the correlation between prevotella bivia colonization density and lps concentration, and to determine the impact of lps on loss of dopamine neurons (da). | 2008 | 18849004 |
| importance of isolation and biotypization of gardnerella vaginalis in diagnosis of bacterial vaginosis. | the natural habitat of gardnerella vaginalis is a vagina since it could be located among 69% of women who have no signs of vaginal infection and in the vagina of as many as 13.5% girls. g. vaginalis is almost certainly identified among women diagnosed with bacterial vaginosis as well as in the urethra of their sexual partner. the increase in prevalence and concentration of g. vaginalis among patients diagnosed with this syndrome confirms that g. vaginalis plays a significant role in its pathogen ... | 2008 | 18816262 |
| prevalence of gardnerella vaginalis in male sexual partners of women with and without bacterial vaginosis. | 2009 | 18797426 | |
| quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study. | the real-time polymerase chain reaction (pcr) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (bv) and candidiasis (ca) was performed. statistical analysis revealed that the bv condition is characterised by a great variability among subjects and that it is associated with a significant increase of prevotella, atopobium, veillonella and gardnerella vaginalis, and a drop in lactobacillus. on the contrary, the vaginal micr ... | 2009 | 18762999 |
| reassessment of bv causation. | 2008 | 18725563 | |
| bacterial vaginosis: a sexually enhanced disease. | 2008 | 18663053 | |
| efficacy and tolerance of metronidazole and miconazole nitrate in treatment of vaginitis. | to evaluate the efficacy and tolerability of a vaginal pessary containing 750 mg of metronidazole and 200 mg of miconazole nitrate used daily for 7 days in the treatment of vaginitis. | 2008 | 18603245 |
| quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems. | transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ... | 2008 | 18579722 |
| cytologically diagnosed gardnerella vaginalis infection and cervical (pre)neoplasia as established in population-based cervical screening. | cervical inflammation has been proposed as a cofactor in the development of cervical cancer. the purpose of this study was to document the prevalence of cervical (pre)neoplastic changes in asymptomatic women with a cytologically diagnosed gardnerella vaginalis infection. | 2008 | 18565490 |
| molecular quantification of gardnerella vaginalis and atopobium vaginae loads to predict bacterial vaginosis. | bacterial vaginosis (bv) is a poorly detected public health problem that is associated with preterm delivery and for which no reliable diagnostic tool exists. | 2008 | 18513147 |
| interrelationships within the bacterial flora of the female genital tract. | analysis of 240 consecutive vaginal swabs using the compatibility profile technique revealed that only 2 bacteria have the ability to be a sole isolate and as such a candidate to be a major aerobic regulator of the bacterial flora of the female genital tract (bffgt). compatibility profiles of lactobacillus and gardnerella vaginalis have shown that these organisms shared compatibility profiling for the majority of the normal bacterial constituents of the female genital tract. dominance disruption ... | 1997 | 18476156 |
| labial and vaginal microbiology: effects of extended panty liner use. | objective: the goals of this study were 1) to better define the labial microflora and 2) to evaluate whether extended non-menstrual use of panty liners would increase genital carriage of undesirable bacteria and predispose to infection.methods: healthy female volunteers (224) were prospectively randomized into panty liner wear groups a (always((r)) deodorant) and b (always((r)) non-deodorant) and into a control group c (no panty liner wear) with instructions for non-menstrual +/- menstrual use > ... | 1997 | 18476146 |
| in vitro ability of the group b streptococci to inhibit gram-positive and gram-variable constituents of the bacterial flora of the female genital tract. | the purpose of this study was to analyze the ability of septicemic and nonsepticemic isolates of group b streptococci (gbs) to inhibit in vitro the principal bacterial groups found in the normal bacterial flora of the female genital tract. | 1995 | 18476028 |
| virulence attributes of low-virulence organisms. | the vast majority of infections involving female pelvic structures arise from organisms that are members of the normal flora. in addition, exogenous organisms that invade through the lower genital tract must interact with organisms that are part of the host's flora. in contrast to the concept that the normal flora is entirely innocuous, recent research has begun to identify what appear to be virulence attributes among these ordinarily low-virulence organisms. most of our understanding of virulen ... | 1994 | 18475373 |
| frequency of infectious agents for vaginitis in patients with a cytological diagnosis of atypical squamous cells of undetermined significance. | to evaluate the presence of infectious agents for vaginitis in patients with ascus. | 2008 | 18459549 |
| antibiotic resistance of gardnerella vaginalis in recurrent bacterial vaginosis. | fifty strains of gardnerella vaginalis isolated from 321 high vaginal swabs over a period of five months were tested for their antibiotic sensitivity. sixty eight per cent of all isolates were resistant to metronidazole while 76% were sensitive to clindamycin. all the strains isolated from cases with recurrence of infection were resistant to metronidazole. clindamycin therapy has a better clinical efficacy than metronidazole in cases of recurrent bacterial vaginosis. | 2008 | 18445953 |
| gardnerella vaginalis and lactobacillus sp in liquid-based cervical samples in healthy and disturbed vaginal flora using cultivation-independent methods. | our objective was to determine the morphotype of the adherent bacteria in liquid-based cytology (lbc) in smears with healthy and disturbed vaginal flora. and to use pcr technology on the same fixed cell sample to establish dna patterns of the 16s rna genes of the bacteria in the sample. thirty samples were randomly selected from a large group of cervical cell samples suspended in a commercial coagulant fixative "(boonfix)." pcr was used to amplify dna of five bacterial species: lactobacillus aci ... | 2008 | 18418885 |
| atopobium vaginae triggers an innate immune response in an in vitro model of bacterial vaginosis. | bacterial vaginosis is the most common vaginal disorder among women of reproductive age. the pathogenesis of bacterial vaginosis is poorly understood, but is defined by a transition in the vaginal flora from the predominant lactobacillus species to other bacterial species such as atopobium vaginae and gardnerella vaginalis. this change is associated with an increase in vaginal cytokine secretion. we hypothesize that vaginal epithelial cells respond to bacterial vaginosis-associated bacteria by t ... | 2008 | 18403235 |
| the polymicrobial hypothesis of bacterial vaginosis causation: a reassessment. | the cause of bacterial vaginosis remains controversial. the two leading hypotheses are that gardnerella vaginalis is the specific aetiologic agent versus the polymicrobial hypothesis that g. vaginalis acts in concert with other bacteria, principally anaerobes, to produce the disease. here we reassess the prevailing polymicrobial hypothesis, finding it conceptually problematical. no host initiating factors as postulated have been identified, nor is there conclusive evidence that g. vaginalis lack ... | 2008 | 18397551 |
| the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh). | in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ... | 2008 | 18397461 |
| functional and phylogenetic characterization of vaginolysin, the human-specific cytolysin from gardnerella vaginalis. | pore-forming toxins are essential to the virulence of a wide variety of pathogenic bacteria. gardnerella vaginalis is a bacterial species associated with bacterial vaginosis (bv) and its significant adverse sequelae, including preterm birth and acquisition of human immunodeficiency virus. g. vaginalis makes a protein toxin that generates host immune responses and has been hypothesized to be involved in the pathogenesis of bv. we demonstrate that g. vaginalis produces a toxin (vaginolysin [vly]) ... | 2008 | 18390664 |
| a retrospective analysis of isolates from patients with vaginitis in a private greek obstetric/gynecological hospital (2003-2006). | vaginitis is a common cause of complaints in women in various parts of the world. through our literature search, we identified noteworthy differences in the etiology of vaginitis based on the geographic location. this study was conducted to describe microbial etiology in a population of symptomatic women with vaginitis in greece. | 2008 | 18376352 |
| [protease and phospholipase activities of candida albicans isolated from vaginal secretions with different ph values]. | even though vulvovaginal candidiasis and bacterial vaginosis are seldom simultaneously found, we have detected this association at an above average frequency. thus, we set out to study the activity of proteinases and phospholipases, virulence factors of candida albicans, to assess their role in the above mentioned association. of a total of 70 candida isolates were retrieved from samples of vaginal secretions analyzed at our diagnostic service, 65 were identified as c. albicans (a group of n=26 ... | 2008 | 18338921 |
| erythema multiforme associated with gardnerella vaginosis and elevated circulating cxcr3+ b cells. | 2008 | 18312462 | |
| placental histological inflammation and reproductive tract infections in a low risk pregnant population in latvia. | to investigate the correlation of reproductive tract infections (rti) and endogenous vaginal flora at first antenatal consultation with placental histological inflammation. | 2008 | 18307078 |
| vaginal il-8 levels are positively associated with candida albicans and inversely with lactobacilli in hiv-infected women. | il-8/cxcl8 is induced during infections, but has not been reported for candida albicans colonization of the female genital tract. cervicovaginal lavage (cvl) samples were collected from 406 hiv-infected women. il-8 levels were evaluated by elisa and compared with levels of c. albicans detected by potassium hydroxide (koh) and pcr. levels of lactobacilli, gardnerella vaginalis and mycoplasma hominis were also determined by pcr. il-8 was significantly higher in samples from women with candida, and ... | 2008 | 18243333 |
| quantitative determination by real-time pcr of four vaginal lactobacillus species, gardnerella vaginalis and atopobium vaginae indicates an inverse relationship between l. gasseri and l. iners. | most studies of the vaginal microflora have been based on culture or on qualitative molecular techniques. here we applied existing real-time pcr formats for lactobacillus crispatus, l. gasseri and gardnerella vaginalis and developed new formats for atopobium vaginae, l. iners and l. jensenii to obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant women aged between 18 and 45 years. | 2007 | 18093311 |
| gardnerella vaginalis bacteremia in a previously healthy man: case report and characterization of the isolate. | gardnerella vaginalis in women causes vaginitis or infections in other sites, such as the urinary tract, but is an infrequent cause of bacteremia. bacteremia in men is very rare and is typically associated with immunocompromised states. here we describe g. vaginalis bacteremia in a previously healthy man with renal calculi and urosepsis. | 2008 | 18057138 |
| an adherent gardnerella vaginalis biofilm persists on the vaginal epithelium after standard therapy with oral metronidazole. | the purpose of this study was to determine the efficacy of standard treatment with oral metronidazole in the eradication of the bacterial vaginosis biofilm. | 2008 | 18005928 |
| isolation of the bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived bacillus amyloliquefaciens. | to purify and characterize an antimicrobial protein (bacteriocin) isolated from the dairy product-derived bacillus amyloliquefaciens. | 2008 | 17976171 |
| [streptococcus group b--association with aerobic vaginitis and ability to human cell lines activation]. | the aim of this study was to estimate: the frequency of aerobic vaginitis, susceptibility of the gbs isolated from vagina of non-pregnant women with and without cervicitis to selected antibiotics and chemotherapeutics and the proinflammatory cytokines production by hela, thp-i, u - 937 cells after stimulation by vaginal gbs. our results indicated low frequency of the aerobic vaginitis -4.5% among non-pregnant young women and ability of the vaginal gbs to release proinflammatory cytokines by huma ... | 2007 | 17929406 |
| rapid detection of atopobium vaginae and association with organisms implicated in bacterial vaginosis. | atopobium vaginae, a fastidious, anaerobic, gram-positive cocci-shaped bacterium that generates large quantities of lactic acid, is associated with bacterial vaginosis (bv). published nucleic acid amplification tests for identifying a. vaginae are directed toward the 16s ribosomal dna with suboptimal specificity and require isolation of the organism. here, sequencing of an a. vaginae genomic library has led to the development of a highly specific and sensitive real-time pcr test for detection of ... | 2008 | 17913456 |
| clinical trial of interconceptional antibiotics to prevent preterm birth: subgroup analyses and possible adverse antibiotic-microbial interaction. | the purpose of this study was to explore whether endometrial microbial colonization and plasma cell endometritis are risk factors for adverse pregnancy outcomes, and whether these outcomes are influenced by interactions between interconceptional antibiotics and the micro-flora. | 2007 | 17904962 |
| bacterial colonization in suspected sexually abused children. | previous studies concluded that symptomatic prepubertal children with gardnerella vaginalis infection should be investigated for child sexual abuse. gardnerella vaginalis is only one out of a group of organism that cause bacterial vaginosis. the aim of the study was to report the frequency of bacterial colonization as well as sexually transmitted diseases and to correlate these data with patient characteristics. | 2007 | 17868895 |
| symptomatic candidiasis: using self sampled vaginal smears to establish the presence of candida, lactobacilli, and gardnerella vaginalis. | in a prospective cohort study, 10 symptomatic women with recurrent vulvovaginal candidiasis were taught how to prepare vaginal smears of their own vaginal fluids on days 7, 14, 21, and 28. the 40 smears were stained with the pas-method and examined by three different cytopathologists for presence of candida. thereafter, the smears were restained with giemsa-stain to determine presence of lactobacilli, gardnerella vaginalis ("clue cells") and neutrophils. all three cytopathologists unequivocally ... | 2007 | 17854078 |
| [vulvovaginitis: vaginal ph changes and associated microflora]. | to establish a correlation between ph vaginal and the microflora associated in carriers of vulvovaginites. | 2005 | 17768818 |
| effect of biofilm phenotype on resistance of gardnerella vaginalis to hydrogen peroxide and lactic acid. | bacterial vaginosis is the most common vaginal disorder worldwide. certain lactobacilli produce h2o2 and lactic acid, which normally suppress growth of anaerobes; however, in bacterial vaginosis, gardnerella vaginalis and other anaerobes proliferate, and the number of lactobacilli decreases. g. vaginalis colonizes the vaginal epithelium as a biofilm, which likely plays a role in colonization and relapsing infection. | 2007 | 17689638 |
| targeted pcr for detection of vaginal bacteria associated with bacterial vaginosis. | several novel bacterial species have been detected in subjects with bacterial vaginosis (bv) by using broad-range pcr assays, but this approach is insensitive for detecting minority species. we developed a series of taxon-directed 16s rrna gene pcr assays for more sensitive detection of key vaginal bacteria. we sought to determine the prevalence of each species in the vagina, its association with bv, and the utility of pcr for the microbiological diagnosis of bv. targeted pcr assays were develop ... | 2007 | 17687006 |
| probiotics for the treatment of women with bacterial vaginosis. | this review considers whether probiotics are effective agents for the treatment and/or prevention of bacterial vaginosis (bv). there seems to be an association between the absence of, or low concentrations of, vaginal lactobacilli and the development of bv. many studies have suggested that the presence of h2o2-producing vaginal lactobacilli may protect against bv, although some studies do not support this hypothesis. in-vitro studies have suggested that certain specific strains of lactobacilli a ... | 2007 | 17633390 |
| alloscardovia omnicolens gen. nov., sp. nov., from human clinical samples. | the taxonomic position of 12 isolates tentatively assigned to the genus bifidobacterium on the basis of a limited phenotypic characterization was examined. the isolates were collected between 1978 and 2005 in belgium, sweden and norway, and originated from various human clinical samples, including urine, blood, urethra, oral cavity, tonsil, and abscesses of lung and aortic valve. on the basis of band number and clustering analysis, repetitive dna element-based pcr fingerprinting using the boxa1r ... | 2007 | 17625172 |
| [the bacterial vaginosis--treatment problems]. | the changes in vaginal ecosystem (decreasing number of h2o2 producing lactobacillus spp. and increasing number of mobiluncus spp., gardnerella vaginalis, atopobium vaginae, prevotella spp., mycoplasma hominis, peptostreptococcus spp.) appears to be a major cause of bacterial vaginosis (bv). bacterial vaginosis is associated with infectious complications in pregnant and non-pregnant women and increasing risk of sti. the preventive role of natural vaginal microflora and problems of bv treatment ar ... | 2007 | 17607971 |
| [conjunctivitis due to gardnerella vaginalis]. | 2007 | 17583660 | |
| altered expression of adhesion molecules in inflammatory cervical smears. | the aim of the present study was to evaluate the expression of pan-cadherin and beta-catenin in cervical smears with various types of infectious agents. | 2008 | 17573909 |
| [frequency of gardnerella vaginalis in vaginal smears of hysterectomized women]. | this work intended to evaluate the frequency of gardnerella vaginallis in cervicalvaginal smears of hysterectomized women when compared to smears of non-hysterectomized women. | 2007 | 17568922 |
| rapid characterization of the normal and disturbed vaginal microbiota by application of 16s rrna gene terminal rflp fingerprinting. | bacterial vaginosis (bv) is a prevalent infection in women of reproductive age associated with numerous sequelae, including preterm delivery, amniotic fluid infections and an increased risk of acquiring sexually transmitted diseases. the vaginal microbiota in bv patients is characterized by a shift from lactobacilli to a diverse spectrum of mostly anaerobic bacteria. in this study, terminal restriction fragment length polymorphism (t-rflp) was used to characterize the vaginal bacterial communiti ... | 2007 | 17510259 |
| [biotypes and antibiotic resistance patterns of gardnerella vaginalis strains isolated from healthy women and women with bacterial vaginosis]. | as gardnerella vaginalis is accepted as a member of normal vaginal flora, it is one of the dominant species which has been related to bacterial vaginosis (bv). the aim of this study was to determine the isolation rate, biotypes and antibiotic resistance patterns of g.vaginalis from the vaginal swab samples of 408 women who were admitted to the outpatient clinics of family planning center. hippurate hydrolysis, lipase and beta-galactosidase tests were performed for biotyping the isolates, and aga ... | 2007 | 17427549 |
| isolation of gardnerella vaginalis from the genital tract of six mares. | 1992 | 17424118 | |
| [progress in the studies of gardnerella vaginal]. | gardnerella vaginal is one of the main pathogenic floras of female bacterial vaginosis. sexually transmitted, it causes fallopian pregnancy, premature rupture of the foetal membrane, neonate premature delivery and other bad pregnancy outcome. gardnerella vaginal is also involved in male urogenital tract infection. this review summarizes the recent advances in the studies of gardnerella vaginal in such aspects as its categorization, biocharacteristics, biogrouping, epidemiology, pathogenesis, dia ... | 2007 | 17393790 |
| the role of gene-environment interaction in predicting adverse pregnancy outcome. | this article focuses on the influence of gene-environment interaction on pregnancy outcome. in particular, we focus on those adverse outcomes related to subclinical infection and the resultant inflammation of gestational tissues. we identify genetic association studies on pregnancy-related disorders with an infectious/inflammatory etiology. all studies in this field have focused on spontaneous preterm delivery and/or preterm and premature rupture of membranes. we discuss those articles where an ... | 2007 | 17369098 |
| modulation of vaginal immune response among pregnant women with bacterial vaginosis by trichomonas vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, and yeast. | this study was undertaken to examine the influence of coinfections on vaginal innate and adaptive immunity, and microbial enzyme activities of pregnant women with bacterial vaginosis (bv). | 2007 | 17306653 |
| [causation and treatment of infectious leucorrhoea at the cocody university hospital (abidjan, côte d'ivoire)]. | to determine the frequency of different bacteria causing infectious leucorrhoea and to calculate the percentage of cures after standard treatment. | 2006 | 17284396 |
| an altered immunity hypothesis for the development of symptomatic bacterial vaginosis. | the hypothesis is advanced that the transition from a lactobacillus-dominated vaginal microflora to a microflora characteristic of bacterial vaginosis (bv), as well as development of the adverse consequences of bv in some women but not in others, are due to alterations in innate immunity. a microbial-induced inhibition of toll-like receptor expression and/or activity may block induction of proinflammatory immunity and lead to the proliferation of atypical vaginal bacteria. a lack of 70-kda heat- ... | 2007 | 17243059 |
| effect of lactobacillus challenge on gardnerella vaginalis biofilms. | bacterial vaginosis (bv) is the most common infectious condition in women. it is caused primarily by anaerobic bacteria which rapidly form biofilms recalcitrant to antibiotic treatment, elevate vaginal ph, induce inflammatory processes and displace indigenous lactobacilli from the vault. gardnerella vaginalis is commonly associated with these infections. microscopy analysis showed that within 72 h, viable g. vaginalis covered a surface area of 567 microm(2), reached a depth of 16 microm and a de ... | 2007 | 17234391 |
| use of dna hybridization test for diagnosing bacterial vaginosis in women with symptoms suggestive of infection. | the purpose of this study was to evaluate a dna hybridization test (affirm vpiii) as an alternative to gram stain for the rapid diagnosis of bacterial vaginosis in women with clinical signs of vaginal infection. vaginal specimens were collected from 321 symptomatic women, and analyzed for bacterial vaginosis by both gram stain using nugent criteria and dna hybridization test. sensitivity, specificity, positive predictive value, and negative predictive value of the dna hybridization test were det ... | 2006 | 17078859 |
| the international infections in pregnancy (iip) study: variations in the prevalence of bacterial vaginosis and distribution of morphotypes in vaginal smears among pregnant women. | the objective of the study was to determine the prevalence of bacterial vaginosis and the distribution of associated morphotypes among asymptomatic pregnant women in different countries. | 2006 | 17074543 |
| epidemiology of sexually transmitted diseases in río cuarto, argentina. | sexually transmitted diseases (std) are a group of transmittable diseases acquired fundamentally through sexual contact. std are a social problem resulting from demographic explosion and changes in sexual conduct, which affects teenagers and adults of all socioeconomic strata. the goal of this work was to establish the actual state of the different std within the studied population. samples of vaginal fluids, endocervical materials and urethral exudates taken from 2,630 patients during five year ... | 2001 | 17061502 |
| treatment of urinary tract infection by gardnerella vaginalis: a comparison of oral metronidazole versus ampicillin. | treatment options for bacterial vaginosis are numerous, however for urinary tract infection (uti) by gardnerella vaginalis have been not reported. our purpose was to compare the efficacy and treatment complications of oral metronidazole versus oral ampicillin for treatment of this condition in a prospective randomized, nonblinded study. fifty-seven women who had symptoms of uti and a positive culture for g. vaginalis were enrolled in the study. only forty-five subjects were considered valuable: ... | 2001 | 17061489 |
| lactobacillus strains isolated from the vaginal microbiota of healthy women inhibit prevotella bivia and gardnerella vaginalis in coculture and cell culture. | the purpose of this study was to investigate how human vaginal isolates of lactobacillus acidophilus, lactobacillus jensenii, lactobacillus gasseri and lactobacillus crispatus inhibit the vaginosis-associated pathogens gardnerella vaginalis and prevotella bivia. results show that all the strains in coculture condition reduced the viability of g. vaginalis and p. bivia, but with differing degrees of efficacy. the treatment of g. vaginalis- and p. bivia-infected cultured human cervix epithelial he ... | 2006 | 17059467 |
| [vaginosis in patients with antenatal foetus death]. | polymerase chain reaction (pcr) analysis of bacterial vaginosis as well as of vaginal anaerobe flora (mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, bacteroides spp. and mobiluncus curtisii) was performed in women with antenatal foetus death. specimens from forty women with this pathology were studied. control group consisted of 100 pregnant women of the adequate age groups and gestation periods, but with live foetus. vaginal smears treated by the use of polymerase chain reac ... | 2006 | 17057305 |