Publications

clostridium histolyticum infection.		1949	18146655
characterization of the gastrointestinal mucosa-associated microbiota of pigs and chickens using culture-based and molecular methodologies.	the microbiota of the gastrointestinal tract (git) can play an important role in the healthy status of farm animals and in the safety of the whole food chain. in this study, the mucosa-associated microbiota of the git of pigs and chickens was analyzed by culture methods and fluorescence in situ hybridization combined with flow cytometry (fcm-fish). in all pig git sections, lactic acid bacteria, enterobacteriaceae, atopobium, bacteroides, and clostridium histolyticum were the predominant bacteria ...	2007	18095433
imbalance in the composition of the duodenal microbiota of children with coeliac disease.	coeliac disease (cd) is the most common immune-mediated enteropathy characterized by chronic inflammation of the small intestinal mucosa. the ingestion of gluten is responsible for the symptoms of cd, but other environmental factors are also thought to play a role in this disorder. in this study, the composition of the duodenal microbiota of coeliac children with active disease, symptom-free cd patients on a gluten-free diet and control children was determined. bacteriological analyses of duoden ...	2007	18033837
structure of the claudin-binding domain of clostridium perfringens enterotoxin.	clostridium perfringens enterotoxin is a common cause of food-borne and antibiotic-associated diarrhea. the toxin's receptors on intestinal epithelial cells include claudin-3 and -4, members of a large family of tight junction proteins. toxin-induced cytolytic pore formation requires residues in the nh(2)-terminal half, whereas residues near the cooh terminus are required for binding to claudins. the claudin-binding cooh-terminal domain is not toxic and is currently under investigation as a pote ...	2008	17977833
in vitro binding of matrix metalloproteinase-2 (mmp-2), mmp-9, and bacterial collagenase on collagenous wound dressings.	chronic wounds are characterized by failure in wound-healing response and a delay in healing or nonclosure of the wounds. this results in a high effort in clinical treatment and/or home care. a major difference between acute wounds and chronic wounds is the imbalance of proteinase inhibitors and proteinase activity that regulates the degradation and regeneration of the extracellular matrix proteins. collagen and collagen/oxidized regenerated cellulose dressings act as a competitive substrate for ...	2007	17650099
antiadhesive effects of mitomycin c and streptopeptidase a in rats with intraperitoneal adhesions.	intraabdominal adhesion formation is a frequent problem after major abdominal surgery. for many years, there have been various attempts to decrease adhesions by using systemic and local drugs and mechanical barriers. in this study we aimed to evaluate the antifibrinolytic antiadhesive effects of mitomycin c (mmc) and streptopeptidase a (sa) against intraabdominal adhesions. forty-eight rats were divided into six groups, each with eight rats. group 1 (sham group) rats were laparotomized by transv ...	2007	17579872
effect of bacterial collagenase on resin-dentin bonds degradation.	the objective of this study is to evaluate the effect of a bacterial collagenase on the degradation of resin-dentin bonds. human dentin surfaces were bonded with: an etch-&-rinse self-priming adhesive (sb), a two-step self-etching primer/adhesive (seb), and a 1-step self-etching adhesive (oub). composite build-ups were constructed. the bonded teeth were stored (24 h, 3 months, 1 year) in distilled water or in a buffered bacterial collagenase solution. half of the specimens were stored as intact ...	2007	17569004
differences between the fecal microbiota of coeliac infants and healthy controls.	coeliac disease (cd) is an immune-mediated enteropathy with a multifactorial aetiology, characterized by chronic inflammation of the small intestinal mucosa. although evidence suggests that the gut microbiota contributes to other chronic inflammatory disorders, its possible role in cd has not been determined. in this study, the composition of the fecal microbiota of coeliac children and age-matched controls was investigated by culture-dependent and -independent methodologies, using fluorescent i ...	2007	17489434
jerusalem artichoke and chicory inulin in bakery products affect faecal microbiota of healthy volunteers.	a study was conducted to test the effects of jerusalem artichoke inulin (ja) or chicory inulin (ch) in snack bars on composition of faecal microbiota, concentration of faecal scfa, bowel habit and gastrointestinal symptoms. forty-five volunteers participated in a double-blind, randomized, placebo-controlled, parallel-group study. at the end of a 7 d run-in period, subjects were randomly assigned to three groups of fifteen subjects each, consuming either snack bars with ch or ja, or snack bars wi ...	2007	17445348
lethal factor of clostridium histolyticum kills cells by apoptosis.	in this study, for the first time, clostridium histolyticum lethal factor was purified by ammonium sulfate precipitation of culture broth, centrifugation through an amicon filter device and hydrophobic interaction chromatography. the purified lethal factor was devoid of proteolytic activity. at a concentration of 150 ng ml(-1) the lethal factor killed 50% of hela cells within 24 h of exposure. abrogation of actin filaments, activation of caspases, fragmentation of nuclear dna as well as ultrastr ...	2007	17328764
analysis of contribution of collagen fibre component in viscoelastic behaviour of periodontal ligament using enzyme probe.	the aims of this study are to observe microscopic changes in the periodontal ligament (pdl) collagen fibres after collagenase treatment, to analyse stress-relaxation behaviour of pdl specimens treated with collagenase, and to elucidate the contribution of the collagen component to the viscoelastic behaviour of the pdl. transverse sections of rat mandibular first molars (n=24) were treated in vitro with 0, 8, 16, or 24 units of bacterial collagenase for 4h at 37 degrees c. histological specimens ...	2007	17266965
factors influencing the collagenase digestion phase of human islet isolation.	substantial advances in human islet isolation technology have occurred during the past decade. however, it is still difficult to recover the entire quantity of islets contained in a pancreas. a major obstacle to successful human islet isolation has been the variability of the collagenase digestion phase of islet isolation. future advances in enzyme technology will make it possible to optimally liberate islets with enzyme blends "tailor-made" for each individual donor pancreas. such innovative st ...	2007	17220782
molecular characterisation of the gut microflora of healthy and inflammatory bowel disease cats using fluorescence in situ hybridisation with special reference to desulfovibrio spp.	inflammatory bowel disease (ibd) is a common cause of chronic large bowel diarrhoea in cats. although the aetiology of ibd is unknown, an immune-mediated response to a luminal antigen is thought to be involved. as knowledge concerning the colonic microflora of cats is limited and requires further investigation, the purpose of this study was to determine the presence of specific bacterial groups in normal and ibd cats, and the potential role they play in the health of the host. total bacterial po ...	2007	17217390
the proteinases of clostridium histolyticum.		1940	16747287
enzymic adaptation in clostridium histolyticum.		1938	16746800
studies on the endo-enzymes, particularly the peptidases, of clostridium histolyticum.		1938	16746799
proteinase secretion and growth of clostridium histolyticum.		1938	16746798
studies on the proteinase of clostridium histolyticum.		1937	16746452
qsar analysis of some 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases and bacterial collagenase.	a quantitative structure-activity relationship (qsar) study has been performed on 5-amino-2-mercapto-1,3,4-thiadiazole based inhibitors of matrix metalloproteinases (mmps) and a bacterial collagenase known as clostridium histolyticum collagenase (chc) to understand the structural features influencing the affinity of these inhibitors towards the enzyme. the compounds in the selected series were characterized by topological and fragmental descriptors calculated using quasar module of molecular ope ...	2006	16682189
prevalence and temporal stability of selected clostridial groups in irritable bowel syndrome in relation to predominant faecal bacteria.	the differences in faecal bacterial population between irritable bowel syndrome (ibs) and control subjects have been reported in several studies. the aim of the present study was to compare the predominant and clostridial faecal microbiota of ibs subjects and healthy controls by applying denaturing gradient gel electrophoresis (dgge) and a recently developed multiplexed and quantitative hybridization-based technique, transcript analysis with the aid of affinity capture (trac). according to the r ...	2006	16585652
some serological aspects of the s-r change in clostridium histolyticum.		1937	16560063
variability in morphological and biochemical properties of clostridium histolyticum (weinberg and seguin).		1937	16560062
proteolysis and the selective destruction of amino acids by clostridium sporogenes and clostridium histolyticum.		1929	16559359
vacuolization of hela cells by a partially purified clostridium histolyticum cytotoxin.	clostridium histolyticum vacuolating cytotoxin was partially purified from culture broth using ammonium sulfate precipitation, gel filtration and hydrophobic interaction chromatography. the toxin caused vacuolization of hela cells visible under a light microscope after 2 h and distinct after 8 h. transmission electron microscopy revealed the presence of numerous vacuoles, condensation of the mitochondrial matrix, increased cytoplasm density and increased amounts of heterochromatin. apoptosis was ...	2006	16553808
effect of a milk formula with prebiotics on the intestinal microbiota of infants after an antibiotic treatment.	antibiotics exert deleterious effects on the intestinal microbiota, favoring the emergence of opportunistic bacteria and diarrhea. prebiotics are nondigestible food components that stimulate the growth of bifidobacteria. our aim was to evaluate the effects on the intestinal microbiota of a prebiotic-supplemented milk formula after an antibiotic treatment. a randomized, double-blind, controlled clinical trial was carried out in 140 infants 1-2 y of age distributed into two groups after a 1-wk amo ...	2006	16492988
new worlds to explore.		2006	16439327
inhibition of collagenase by naturally-occurring flavonoids.	we examined the inhibitory activities of various flavonoids, including the flavanones, flavones/isoflavones and flavonols, on collagenase from clostridium histolyticum to establish their therapeutic potential against skin inflammation and photoaging. in general, the flavonols were stronger inhibitors than the flavones/isoflavones, and this indicated the importance of the c-3 hydroxyl substitution. quercetin was the most active flavonoid among those tested, and it showed an ic50 of 286 microm. th ...	2005	16276971
differences between the gut microflora of children with autistic spectrum disorders and that of healthy children.	children with autistic spectrum disorders (asds) tend to suffer from severe gastrointestinal problems. such symptoms may be due to a disruption of the indigenous gut flora promoting the overgrowth of potentially pathogenic micro-organisms. the faecal flora of patients with asds was studied and compared with those of two control groups (healthy siblings and unrelated healthy children). faecal bacterial populations were assessed through the use of a culture-independent technique, fluorescence in s ...	2005	16157555
determination of the cytotoxic effect of clostridium histolyticum culture supernatant on hela cells in the presence of protease inhibitors.	clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. this includes lethal toxin, clostripain and high-potassium-sensitive toxin. since the number of c. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of c. histolyticum. in this study we evaluated in vitro the influe ...	2005	16051065
[substrate specificity of collagenase of streptomyces sp. 1349 and keratinase of streptomyces sp. 1382].	the paper determines the action specificity concerning the bonding type of collagenases of strepomyces sp. 1349 and keratinases of streptomyces sp. 1382. experimental data obtained evidence for the wide specificity of the obtained enzymatic drugs. it has been established that both collagenases and keratinases display high specificity in respect of the bonds made by the residues of hydrophobic amino acids. collagenases of streptomyces sp. 1349, as to their wide action specificity, differed from c ...	2005	16018211
a novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo.	prebiotics are nondigestible food ingredients that encourage proliferation of selected groups of the colonic microflora, thereby altering the composition toward a more beneficial community. in the present study, the prebiotic potential of a novel galactooligosaccharide (gos) mixture, produced by the activity of galactosyltransferases from bifidobacterium bifidum 41171 on lactose, was assessed in vitro and in a parallel continuous randomized pig trial. in situ fluorescent hybridization with 16s r ...	2005	15987856
modulation of the fecal microbiota by the intake of a lactobacillus johnsonii la1-containing product in human volunteers.	lactobacillus johnsonii la1 (la1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. this study evaluates how the ingestion of different amounts of la1 influences the main bacterial populations of the fecal microbiota. eight asymptomatic volunteers participated in the study. after a basal period, they ingested daily 100 ml of a product containing 10(8) cfu ml(-1) of la1 during the first week, 200 ml during the second week a ...	2005	15970400
an unusual cause of soft tissue infection.		2005	15951543
improved enzymatic isolation of fibroblasts for the creation of autologous skin substitutes.	the number of medical applications using autologous fibroblasts is increasing rapidly. we investigated thoroughly the procedure to isolate cells from skin using the enzymatic tissue dissociation procedure. tissue digestion efficiency, cell viability, and yield were investigated in relation to size of tissue fragments, digestion volume to tissue ratio, digestion time, and importance of other protease activities present in clostridium histolyticum collagenase (chc) (neutral protease, clostripain, ...	2004	15723562
quantum theoretic qsar of benzene derivatives: some enzyme inhibitors.	our previously developed approach to the development of qsar equations for benzene derivatives, originally for phenylalkylamine hallucinogens, has been applied to four new systems: sulfonamide inhibitors of the enzymes carbonic anhydrase, thrombin, trypsin, and clostridium histolyticum collagenase. the novel features involve the energies and nodal orientations of pi-like orbitals, and an allowance for the symmetry of the benzene nucleus. the resulting equations give better fits, better predictiv ...	2004	15499995
factor xiii contrasts the effects of metalloproteinases in human dermal fibroblast cultured cells.	matrix metalloproteinases (mmps) are overexpressed in venous leg ulcers, determining a breakdown of the main extracellular matrix (ecm) components owing mainly to collagenase activities, and so playing a crucial role in ulcer pathogenesis. the authors studied the effects of coagulation factor xiii (fxiii), which cross-links collagen and other ecm components, in human fibroblast cultured cells in the presence and in the absence of matrix metalloproteinases from clostridium histolyticum collagenas ...	2004	15490040
production, purification, and some properties of clostridium histolyticum collagenase.		1950	15440885
outbreak of clostridium histolyticum infections in injecting drug users in england and scotland.	clostridial infections in injecting drug users in the united kingdom are a relatively new phenomenon that came to light in 2000 when cases of serious illness and deaths due to clostridium novyi were recorded. in the period december 2003 to april 2004, the anaerobe reference laboratory received twelve referrals of an extremely rare isolate, clostridium histolyticum, from cases of infection in injecting drug users submitted from nine different hospitals in england and scotland. molecular typing of ...	2004	15381836
interaction of aldehydes with collagen: effect on thermal, enzymatic and conformational stability.	stabilization of type i rat tail tendon (rtt) collagen by various aldehydes, viz. formaldehyde, gluteraldehyde, glyoxal and crotanaldehyde was studied to understand the effect of each on the thermal, enzymatic and conformational stability of collagen. the aldehydes have been found to increase the heat stability of rat tail tendon collagen fibres from 62 to 77-86 degrees c. the increase in thermal stability was found to be in a species dependent manner. the variation in the thermal stability of c ...	2004	15374680
quantitative analysis of the impact of the organic matrix on the fluoride effect on erosion progression in human dentine using longitudinal microradiography.	after an initial demineralisation, an intensive fluoridation is capable of inhibiting the erosive mineral loss in dentine completely, which might be related to the presence of the exposed organic dentine matrix. aim of the present study was, therefore, to evaluate whether fluoride is also effective when the organic material is removed. the study was a cyclic de- and remineralisation model over 5 days in vitro. samples from human coronal dentine were demineralised with citric acid (ph 2.3; 6 x 10 ...	2004	15353250
inhibition effects of (+)-catechin-aldehyde polycondensates on proteinases causing proteolytic degradation of extracellular matrix.	inhibition effects of (+)-catechin-aldehyde polycondensates against the activity of proteinases, clostridium histolyticum collagenase (chc) and human neutrophil elastase (hne) causing proteolytic degradation of extracellular matrix (ecm), have been investigated. in normal tissues, a balance is reached between the formation and destruction of ecm, leading to a state of homeostasis. however, uncontrolled destruction of ecm contributes to tumor invasion and metastasis. in the measurement of the inh ...	2004	15207729
[the influences of lanthanum,cerium and fluorine ions to the activity of collagenase degrading cemental collagens]	the influences of trace elements lanthanum,cerium and fluorine to the activity of clostridium histolyticum collagenase(chc) during the period of chc degrading the cemental root collagen in vitro is studied.the results reveal that la(3+) can significantly inhibit the activity of the chc than ce(3+) and f(-). therefore it suggests that lanthanum may be used as a new kind of element in preventing root caries.the mechanisms of above functions are also discussed.	1997	15159961
the structure-function relationship in the clostripain family of peptidases.	in this study we investigate the active-site structure and the catalytic mechanism of clostripain by using a combination of three separate techniques: affinity labelling, site-directed mutagenesis and molecular modelling. a benzamidinyl-diazo dichlorotriazine dye (bdd) was shown to act as an efficient active site-directed affinity label for clostridium histolyticum clostripain. the enzyme, upon incubation with bdd in 0.1 m hepes/naoh buffer ph 7.6, exhibits a time-dependent loss of activity. the ...	2004	15009210
the serological identification of cultures of clostridium histolyticum as group iii of the proteolytic clostridia.		1952	14888966
the alpha, beta and gamma antigens of clostridium histolyticum (weinberg & séguin, 1916).		1950	14778941
enzymes of clostridium welchii type a and clostridium histolyticum that disintegrate decalcified human tooth dentine.		1950	14778940
protease inhibitors: synthesis of bacterial collagenase and matrix metalloproteinase inhibitors incorporating succinyl hydroxamate and iminodiacetic acid hydroxamate moieties.	a series of succinyl hydroxamates/bishydroxamates as well as a new structural type of matrix metalloproteinase (mmp)/bacterial protease (bp) inhibitors, incorporating iminodiacetic (ida) hydroxamate/bishydroxamate moieties, has been synthesized and tested for interaction with four vertebrate proteases, mmp-1, mmp-2, mmp-8 and mmp-9, and a bp, the collagenase isolated from clostridium histolyticum (chc). the new derivatives generally showed inhibition constants in the range of 8-62 nm against the ...	2003	14506914
toxicity of enzymes from clostridium histolyticum.		1960	14403502
[on proteins and their reduction products. xvi. on the specificity of collagenase from clostridium histolyticum and its application to the determination of the primary structure of collagen. (preliminary report)].		1959	14401656
debridement of experimental burns with clostridium histolyticum h-4 enzymes.		1960	14399293
multiplicity of clostridium histolyticum collagenases.		1964	14240635
enzymatic debridement of third-degree burns on guinea pigs by clostridium histolyticum proteinases.	webster, marion e. (walter reed army institute of research, washington, d. c.), patricia l. altieri, david a. conklin, sanford berman, joseph p. lowenthal, and raymond b. gochenour. enzymatic debridement of third-degree burns on guinea pigs by clostridium histolyticum proteinases. j. bacteriol. 83:602-608. 1962.-an attempt has been made to correlate in vitro activities of clostridium histolyticum h-4 proteinases, as measured against azocasein, azocoll, gelatin, and collagen, with their ability t ...	1962	14005493
the interactions between clostridium septicum and clostridium histolyticum toxins and antitoxins.		1962	13983973
[stability behavior of collagenase from clostridium histolyticum].		1963	13938916
debridement of burns on various animal species by clostridium histolyticum proteinases.		1962	13899369
[experimental inoculation of the chick embryo with clostridium histolyticum].		1961	13884989
large scale purification of proteinases from clostridium histolyticum filtrates.	conklin, david a. (walter reed army institute of research, washington, d. c.), marion e. webster, patricia l. altieri, sanford berman, joseph p. lowenthal, and raymond b. gochenour. large scale purification of proteinases from clostridium histolyticum filtrates. j. bacteriol. 82:589-594. 1961.-a method for the large scale preparation and partial purification of clostridium histolyticum proteinases by fractional precipitation with ammonium sulfate is described. conditions for adequate separation ...	1961	13880849
factors affecting the elaboration by clostridium histolyticum of proteinases capable of debriding third degree burn eschars on guinea pigs.	berman, sanford (walter reed army institute of research, washington, d. c.), joseph p. lowenthal, marion e. webster, patricia l. altieri, and raymond b. gochenour. factors affecting the elaboration by clostridium histolyticum of proteinases capable of debriding third degree burn eschars on guinea pigs. j. bacteriol. 82:582-588. 1961.-a comparison of three strains of clostridium histolyticum grown in two different media indicated that the h-4 strain produced the greatest yield of enzyme(s) capabl ...	1961	13867979
electron microscopic studies on ultrathin sections of spores of clostridium tetani and clostridium histolyticum, with special reference to sporulation and spore germination process.		1960	13774854
[clostripain, a protease from clostridium histolyticum. 1. purification activation by thiols].		1960	13758027
[on proteins and their decomposition products. 18. the hydrolysis of synthetic and natural substrates by collagenase from clostridium histolyticum].		1960	13714094
[clostripain, a protease from clostridium histolyticum. 2. specificity].		1960	13708985
[research on the odors emitted by anaerobic bacteria. iii. clostridium histolyticum].		1961	13701340
the use of clostridium histolyticum enzymes in the treatment of experimental third degree burns.		1959	13675991
studies on the collagenases of clostridium histolyticum.		1959	13661949
[study of immunological phenomena by means of tissue culture. iv. cytopathogenic effects of clostridium histolyticum toxin].		1959	13658453
[studies on clostridium histolyticum anatoxins using zone electrophoresis and counter diffusion on agar].		1959	13636341
clostridium histolyticum collagenase: its purification and properties.		1958	13534676
serological evidence for a specific clostridium histolyticum gelatinase.		1958	13525623
the cysteine-inactivated proteinase (alpha-antigen) of clostridium histolyticum.		1958	13525622
new proteolytic enzymes from clostridium histolyticum filtrates.		1958	13525621
effects of clostridium histolyticum enzymes upon burned and unburned tissues in vitro.		1958	13521674
[purification and chemical properties of anatoxin of clostridium histolyticum].		1957	13499480
nutritional studies with clostridium histolyticum.		1957	13462971
exopeptidases of clostridium histolyticum.		1957	13445228
[not available].		1956	13395010
pharmacologically-active polypeptide formed from blood globulin by a cysteine-activated protease from clostridium histolyticum.		1956	13373851
[ammonia production and toxicity of clostridium histolyticum cultured in a gelatinated medium].		1956	13363038
[the proteolytic enzymes of clostridium histolyticum; influence of complexon iii and of calcium on the stability of two of these enzymes].		1955	13260245
separation of proteolytic enzymes of clostridium histolyticum.		1954	13223777
[studies on the properties of clostridium histolyticum and clostridium septicum antisera].		1953	13124941
the haemolysin of clostridium histolyticum.		1953	13106224
the activity of clostridium histolyticum proteinase on synthetic substrates.		1953	13031635
a comparison of the lethal and hemolytic toxins of clostridium histolyticum.		1952	13006411
the partial purification of the lethal toxin of clostridium histolyticum.		1952	13006410
a cysteine-activated proteinase of clostridium histolyticum.		1952	13000153
a quantitative structure-activity relationship study on clostridium histolyticum collagenase inhibitors: roles of electrotopological state indices.	a quantitative structure-activity relationship (qsar) study has been made on eight different series of clostridium histolyticum collegenase (chc) inhibitors. these series are comprised of four different groups of sulfonylated amino acids and their corresponding hydroxamates. in each series, the inhibition potency of the compounds has been found to be significantly correlated with the electrotopological state (e-state) indices of nitrogen and sulfur atoms of the sulfonylated amino group in the mo ...	2003	12818668
a comparative qsar study on carbonic anhydrase and matrix metalloproteinase inhibition by sulfonylated amino acid hydroxamates.	a quantitative structure-activity relationship (qsar) study is made on the inhibition of a few isozymes of carbonic anhydrase (ca) and some matrix metalloproteinases (mmps), both zinc containing families of enzymes, by sulfonylated amino acid hydroxamates. for both enzymes, the inhibition potency of the hydroxamates is found to be well correlated with kier's first-order valence molecular connectivity index 1chi(v) of the molecule and electrotopological state indices of some atoms. from the resul ...	2003	12751815
protease inhibitors: synthesis of bacterial collagenase and matrix metalloproteinase inhibitors incorporating arylsulfonylureido and 5-dibenzo-suberenyl/suberyl moieties.	novel matrix metalloproteinase (mmp)/bacterial collagenase inhibitors are reported, considering the sulfonylated amino acid hydroxamates as lead molecules. a series of compounds was prepared by reaction of arylsulfonyl isocyanates with n-(5h-dibenzo[a,d]cyclohepten-5-yl)- and n-(10,11-dihydro-5h-dibenzo[a,d]cyclohepten-5-yl) methyl glycocolate, respectively, followed by the conversion of the coome to the carboxylate/hydroxamate moieties. the corresponding derivatives with methylene and ethylene ...	2003	12713832
a bacterial collagen-binding domain with novel calcium-binding motif controls domain orientation.	the crystal structure of a collagen-binding domain (cbd) with an n-terminal domain linker from clostridium histolyticum class i collagenase was determined at 1.00 a resolution in the absence of calcium (1nqj) and at 1.65 a resolution in the presence of calcium (1nqd). the mature enzyme is composed of four domains: a metalloprotease domain, a spacing domain and two cbds. a 12-residue-long linker is found at the n-terminus of each cbd. in the absence of calcium, the cbd reveals a beta-sheet sandwi ...	2003	12682007
collagenase-induced changes in articular cartilage as detected by electron-microscopic stereology, quantitative polarized light microscopy and biochemical assays.	the purpose of this study was to compare the ability of electron-microscopic (em) stereology with quantitative polarized light microscopy (plm) and biochemical collagen (hydroxyproline) and crosslink (pyridinoline) analyses to detect changes in the superficial collagen network of bovine articular cartilage after digestion in vitro with purified bacterial (clostridium histolyticum) collagenase (30 u/ml) for 24 and 48 h. collagen volume (v(v)) and surface (s(v)) densities of the uppermost third of ...	2002	12566629
a quantitative structure-activity relationship study on some matrix metalloproteinase and collagenase inhibitors.	a quantitative structure-activity relationship (qsar) study is made on some hydroxamic acid-based inhibitors of matrix metalloproteinases (mmps) and a bacterial collagenase, namely clostridium histolyticum collagenase (chc), that also belongs to an mmp family, m-31, using kier's valence molecular connectivity index (1)chi(v) of the substituents and electrotopological state (e-state) indices of some atoms. the results indicate that out of the four mmps (mmp-1, mmp-2, mmp-8, and mmp-9) studied, mm ...	2003	12517437
inhibition of collagenase and metalloproteinases by aloins and aloe gel.	the effects of aloe barbadensis gel and aloe gel constituents on the activity of microbial and human metalloproteinases have been investigated. clostridium histolyticum collagenase (chc) results dose-dependently inhibited by aloe gel and the activity-guided fractionation led to an active fraction enriched in phenolics and aloins. aloins have been shown to be able to bind and to inhibit chc reversibly and non-competitively. aloe gel and aloins are also effective inhibitors of stimulated granulocy ...	2003	12479983
pcr detection of clostridium chauvoei in pure cultures and in formalin-fixed, paraffin-embedded tissues.	the polymerase chain reaction (pcr) was used to amplify specific segments of the 16s ribosomal rna gene of clostridium chauvoei, a major pathogen of ruminants. three sets of primers were used to produce amplicons of 159, 836 and 959 base pairs (bp), respectively. the pcr was evaluated by testing clinically important strains of clostridium, including 21 strains of c. chauvoei, five strains each of clostridium septicum and clostridium perfringens and two strains each of clostridium novyi, clostrid ...	2003	12458172
mucosal and invading bacteria in patients with inflammatory bowel disease compared with controls.	endogenous intestinal bacteria and/or specific bacterial pathogens are suspected of being involved in the pathogenesis of inflammatory bowel diseases (ibd). the aim of this study was to investigate ibd tissues for different bacterial population groups harbouring the mucosal surface and/or invading the mucosa.	2002	12374228
free fatty acids inhibit the activity of clostridium histolyticum collagenase and human neutrophil elastase.	we investigated the ability of free fatty acids to inhibit the activity of clostridium histolyticum collagenase (ec 3.4.24.3) and human neutrophil elastase (ec 3.4.21.37). we determined the activity of collagenase by degradation of resorufin-labeled casein fluorimetrically. the determination of the elastase activity was performed by a spectrophotometric method using a 4-nitroanilide peptide substrate. we found that most of the tested fatty acids inhibited collagenase at concentrations between 50 ...	2002	12357383
protease inhibitors: synthesis of matrix metalloproteinase and bacterial collagenase inhibitors incorporating 5-amino-2-mercapto-1,3,4-thiadiazole zinc binding functions.	matrix metalloproteinase (mmp)/bacterial collagenase inhibitors incorporating 5-amino-2-mercapto-1,3,4-thiadiazole zinc binding functions are reported. a series of compounds was prepared by reaction of arylsulfonyl isocyanates or arylsulfonyl halides with phenylalanyl-alanine, followed by coupling with 5-amino-2-mercapto-1,3,4-thiadiazole in the presence of carbodiimides. these new compounds were assayed as inhibitors of human mmp-1, mmp-2, mmp-8 and mmp-9, and of the collagenase isolated from t ...	2002	12217351
bacterial protease inhibitors.	serine-, cysteine-, and metalloproteases are widely spread in many pathogenic bacteria, where they play critical functions related to colonization and evasion of host immune defenses, acquisition of nutrients for growth and proliferation, facilitation of dissemination, or tissue damage during infection. since all the antibiotics used clinically at the moment share a common mechanism of action, acting as inhibitors of the bacterial cell wall biosynthesis or affecting protein synthesis on ribosome ...	2002	12111749
screening of selected basidiomycetes for inhibitory activity on clostridium histolyticum collagenase and human leukocyte elastase.	collagenase and elastase play a critical role in the degradation of connective tissue. aqueous and dichloromethane extracts of 15 basidiomycetes were screened for their ability to inhibit the activity of collagenase and elastase. collagenase (ec 3.4.24.3) was not inhibited by aqueous extracts, but by dichloromethane extracts in concentrations of 200 microg/ml. for seven extracts an ic(50) less than 200 microg/ml could be observed. elastase (3.4.21.37) was inhibited by both aqueous and dichlorome ...	2002	11933148
collagen-binding domain of a clostridium histolyticum collagenase exhibits a broad substrate spectrum both in vitro and in vivo.	the substrate spectrum of the tandem collagen-binding domain (cbd) of clostridium histolyticumclass i collagenase (colg) was examined both in vitro and in vivo. cbd bound to insoluble type i, ii, iii and iv collagens in vitro, and to skin, aorta, tendon, kidney, trachea and corneal tissues containing various types of collagen fibrils or sheets. cbd bound to all kinds of collagen fibrils regardless of their diameters and also bound to sheet-forming collagen in the glomerular basal lamina or desce ...	2001	11913772