Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| black teeth: beauty or caries prevention? practice and beliefs of the kammu people. | to be beautiful and caries-free, kammu women in laos and vietnam habitually paint their teeth black. although this practice existed for many generations, it is now known only among the elderly. | 2006 | 16515671 |
| anticariogenic activity of macelignan isolated from myristica fragrans (nutmeg) against streptococcus mutans. | the occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic streptococcus mutans. preliminary antibacterial screening revealed that the extract of myristica fragrans, widely cultivated for the spice and flavor of foods, possessed strong inhibitory activity against s. mutans. the anticariogenic compound was successfully isolated from the methanol extract of m. fragrans by repeated silica gel chromatography, and its structure was identified as macelignan by inst ... | 2006 | 16492529 |
| in vitro antibacterial activity of the peptide psvp-10 against streptococcus mutans and streptococcus sobrinus with and without glycocalyx. | the antibacterial activity of the peptide psvp-10 obtained from pseudomonas sp. r10 against streptococcus mutans and streptococcus sobrinus was investigated. one hundred and twenty strains of s. mutans and 120 strains of s. sobrinus with and without glycocalyx were isolated from saliva samples in trypticase-yeast-cysteine-sucrose-bacitracin (tycsb) agar. bacterial identification was made by polymerase chain reaction. glycocalyx production was observed in modified tycsb agar and confirmed with a ... | 2006 | 16472991 |
| photosensitization of in vitro biofilms by toluidine blue o combined with a light-emitting diode. | in natural ecosystems, micro-organisms grow preferentially attached to surfaces, forming matrix-enclosed biofilms. the aim of this study was to determine photodestruction levels in biofilms after subjecting them to photodynamic therapy. biofilms of streptococcus mutans, s. sobrinus, and s. sanguinis were grown on enamel slabs for 3, 5 or 7 d. both the number of viable micro-organisms and the concentration of water-insoluble polysaccharides were analysed, and mineral loss (deltaz) analyses were p ... | 2006 | 16460343 |
| cariogenic virulence characteristics of mutans streptococci isolated from caries-active and caries-free adults. | the objective of the study was to compare aciduricity (ability, to live in acid), acidogenicity (ability to produce acid), and intracellular polysaccharide production of mutans streptococci (ms) strains isolated from caries-active (ca, with one or more cavitated lesions) and caries-free (cf, with no clinically observable new caries in the last five years) adults. forty-three ms strains from 17 of 17 ca adults, and 14 strains from eight of 12 cf adults were investigated. ms isolates' growth, surv ... | 2005 | 16454241 |
| effect of mixed mutans streptococci colonization on caries development. | to evaluate the clinical importance of mixed mutans streptococci colonization in predicting caries in preschool children. | 2006 | 16390341 |
| influence of biosurfactant on interactive forces between mutans streptococci and enamel measured by atomic force microscopy. | although interactive forces, influenced by environmental conditions, between oral bacteria and tooth surfaces are important for the development of plaque, they have never been estimated. it is hypothesized that interactive forces, as measured by atomic force microscopy, between enamel with or without a pellicle and two strains of mutans streptococci become less attractive by the application of a streptococcus mitis bms biosurfactant coating. upon approach of each of the strains toward bare and p ... | 2006 | 16373681 |
| cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica. | cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (cis). when chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. testing of the adsorption and desorption ability of cis using various microbes such as escherichia coli, aeromonas hydrophila, pseudomonas aeruginosa, bacillus subtilis, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, ... | 2005 | 16332887 |
| inhibitory activity of glass-ionomer cements on cariogenic bacteria. | this study evaluated the antibacterial activity of the glass-ionomer cements vitrebond (3m espe), ketac molar (3m espe) and fuji ix (gc america) against s mutans, s sobrinus, l acidophilus and a viscosus, using the agar diffusion test. inocula were obtained by the seed of indicators cultures in bhi broth incubated at 37 degrees c for 24 hours. base layers containing 15 ml of bhi agar and 300 microl of each bacteria suspension were prepared in petri dishes. six wells measuring 4 mm in diameter we ... | 2005 | 16268399 |
| synergistic inhibition by combination of fluoride and xylitol on glycolysis by mutans streptococci and its biochemical mechanism. | the purpose of this study was to evaluate the combined inhibitory effect of fluoride and xylitol on acid production by mutans streptococci, streptococcus mutans nctc10449 and streptococcus sobrinus 6715, from glucose under strictly anaerobic conditions at fixed ph 5.5 and 7.0. the bacteria were grown in a tryptone-yeast extract broth under strictly anaerobic conditions (n2: 80%; h2: 10%; co2: 10%). reaction mixtures for acid production from glucose contained bacterial cells with fluoride (0-6.4 ... | 2005 | 16251799 |
| differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | it is difficult to distinguish mutans streptococci on the species level, and even more so on the subspecies level. intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) (icm) was applied to reference strains of five of the species of the mutans group (streptococcus criceti, streptococcus downei, streptococcus mutans, streptococcus ratti, streptococcus sobrinus), nonmutans streptococci (streptococcus oralis, streptococcus mitis, streptococcus sali ... | 2005 | 16101961 |
| quantitative analysis of the adhesion of cariogenic streptococci to orthodontic metal brackets. | the aim of this study was to analyze the adhesion of cariogenic streptococci to orthodontic metal brackets in terms of the type of bacterial strains, the incubation time, and saliva coating. two strains of streptococcus mutans (s. mutans lm7 and s. mutans omz65) and two strains of s. sobrinus (s. sobrinus b13 and s. sobrinus 6715) were used. twenty metal brackets were incubated with either unstimulated whole saliva or phosphate-buffered saline for two hours. the bacterial adhesion assays were th ... | 2005 | 16097239 |
| levels of mutans streptococci and lactobacilli in plaque on aged restorations of an ion-releasing and a universal hybrid composite resin. | the purpose of this in vivo study was to evaluate the cariogenic microflora of plaque on aged restorations of a hydroxyl, fluoride, and calcium ion-releasing composite resin (ircr) (ariston phc), and to compare it intra-individually with a universal hybrid composite resin and enamel. each of 19 subjects received one proximal restoration of the ircr, one proximal universal hybrid composite resin restoration (cr) and each subject had one non-filled proximal enamel control surface to make intra-ind ... | 2005 | 16095058 |
| the influence of a novel propolis on mutans streptococci biofilms and caries development in rats. | a flavonoids-free brazilian propolis (type 6) showed biological effects against mutans streptococci and inhibited the activity of glucosyltransferases. this study evaluated the influence of the ethanolic extract of a novel type of propolis (eep) and its purified hexane fraction (eeh) on mutans streptococci biofilms and the development of dental caries in rats. the chemical composition of the propolis extracts were examined by gas chromatography/mass spectrometry. the effects of eep and eeh on st ... | 2006 | 16054589 |
| tansmission, diversity and virulence factors of sreptococcus mutans genotypes. | dental caries is an infectious and transmissible disease, in which many genetic, environmental and behavioral risk factors interact. the mutans streptococci (ms), mainly streptococcus mutans and streptococcus sobrinus are the microorganisms most strongly associated with this disease. the main virulence factors associated with ms cariogenicity include adhesion, acidogenicity and acid tolerance. these properties work together to modify the physico-chemical properties of the biofilm, resulting in e ... | 2005 | 16050484 |
| effect of xylitol-containing chewing gums on interdental plaque-ph in habitual xylitol consumers. | the aim was to investigate the effect of high and low amounts of xylitol on the interdental plaque-ph, directly and after sucrose challenge, in schoolchildren with habitual consumption. | 2005 | 16040446 |
| antibacterial activities and bonding of mmsa/tbb resin containing amphiphilic lipids. | the purpose of this study was to investigate the antibacterial activity of mma/tbb resin containing newly developed amphiphilic lipids. the amphiphilic lipids, c10-l-ala/pts and c12-l-ala/pts, synthesized from the reaction of n-alkyl alcohol and l-alanine were dissolved in mma at concentrations of 0.5, 1.0, 1.5, and 2.0 mol%. resin mixtures of pmma powder and each mma liquid containing lipid and tbb were prepared for all tests. both lipids gave antibacterial effect to mma/ tbb resin. the additio ... | 2005 | 16022446 |
| the complex oral microflora of high-risk individuals and groups and its role in the caries process. | the involvement of the oral biofilm in the caries process requires re-evaluation. the essential role of mutans streptococci (streptococcus mutans and streptococcus sobrinus) in the caries process is not proven. acid production by dental plaque is not dependent upon the presence of mutans streptococci; caries occurs in the absence of these species and their presence does not necessarily indicate caries activity. other oral bacteria, non-mutans streptococci, actinomyces spp. and bifidobacterium sp ... | 2005 | 16008631 |
| effects of sanguinaria in fluoride-containing dentifrices on the remineralisation of subsurface carious lesion in vitro. | the purpose of this study was to investigate the potential cariostatic benefits of dentifrice containing both fluoride (f) and sanguinaria (sg) in a silica abrasive formula using the microbial de- and re-mineralisation cycle model. | 2005 | 15997962 |
| salivary actinomyces naeslundii genospecies 2 and lactobacillus casei levels predict pregnancy outcomes. | gravida's poor periodontal health is emerging as a modifiable independent risk factor for preterm delivery and low birth weight. | 2005 | 15974839 |
| effect of acidulated phosphate fluoride (apf) gel on the adherence of cariogenic bacteria to resin composites. | to evaluate the effect of 1.23% acidulated phosphate fluoride (apf) and the subsequent polishing on the in vitro adherence of mutans streptococci to resin composites in a model that simulated the oral cavity conditions. | 2005 | 15973825 |
| longitudinal study of dental caries incidence associated with streptococcus mutans and streptococcus sobrinus in pre-school children. | streptococcus mutans and streptococcus sobrinus are known to be associated with the development of dental caries. in this study these bacteria were detected in pre-school children (each with primary dentition, age range 3-5 years, n = 60) using a pcr method, and then their presence was compared with the incidence of dental caries over a 1-year period. plaque samples were collected from all erupted tooth sites using a sterile toothbrush. dental examinations at the beginning of the study (baseline ... | 2005 | 15947431 |
| in vitro and in vivo effects of isolated fractions of brazilian propolis on caries development. | recently, two chemically different types of brazilian propolis (type-3 and -12) were shown to have cariostatic properties. this study aimed to evaluate the influence of their isolated fractions on mutans streptococci viability, glucosyltransferases (gtfs) activity and caries development in rats. the ethanolic extracts of propolis (eeps) were serially fractionated into hexane (h-fr), chloroform, ethyl acetate, and ethanol. the ability of the four fractions and eep to inhibit streptococcus mutans ... | 2005 | 15913934 |
| effects of disinfectants on resilient denture-lining materials contaminated with staphylococcus aureus, streptococcus sobrinus, and candida albicans. | resilient denture-lining materials are more susceptible to microbial adhesion than are denture base acrylic resin. as denture hygiene is essential to maintain the serviceability of the denture, disinfectant solutions have been suggested as a method for effectively cleaning resilient liners. the aim of this study was to determine the effectiveness of four different disinfectant solutions on four different resilient lining materials contaminated with staphylococcus aureus, streptococcus sobrinus, ... | 2005 | 15892535 |
| susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells. | antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated. | 2005 | 15886266 |
| chemical compositions and antimicrobial activities of four different anatolian propolis samples. | propolis means a gum that is gathered by bees from various plants. it is known for its biological properties, having antibacterial, antifungal and healing properties. the aims of this study were to evaluate the antimicrobial activity of four different anatolian propolis samples on different groups of microorganisms including some oral pathogens and comparison between their chemical compositions. ethanol extracts of propolis (eep) were prepared from four different anatolian propolis samples and e ... | 2005 | 15881836 |
| six-month polyol chewing-gum programme in kindergarten-age children: a feasibility study focusing on mutans streptococci and dental plaque. | to investigate the use of polyol-containing chewing gums in a day-care centre (kindergarten) setting as a means to affect the growth of mutans streptococci and dental plaque. | 2005 | 15880962 |
| immunological and protective effects of diepitopic subunit dental caries vaccines. | as a prelude to development of broader-spectrum vaccines for dental caries, we explored the immune potential of constructs combining epitopes from mutans streptococcal glucosyltransferases (gtf) and glucan binding protein b (gbpb). two diepitopic peptide constructs were synthesized in a multiple antigenic peptide (map) format. both constructs contained syi, a 20-mer gbpb peptide that included a sequence having major histocompatibility complex class ii binding characteristics. one diepitopic cons ... | 2005 | 15845483 |
| optimization of conditions for the efficient production of mutan in streptococcal cultures and post-culture liquids. | the strain streptococcus sobrinus ccug 21020 was found to produce water-insoluble and adhesive mutan. the factors influencing both stages of the mutan production, i.e. streptococcal cultures and glucan synthesis in post-culture supernatants were standardized. the application of optimized process parameters for mutan production on a larger scale made it possible to obtain approximately 2.2 g of water-insoluble glucan per 11 of culture supernate--this productivity was higher than the best reported ... | 2005 | 15813222 |
| [mgb probes detect streptococcus mutans and streptococcus sobrinus in real time]. | to detect and distinguish streptococcus mutans (s. mutans) and streptococcus sobrinus (s. sobrinus) quickly in epidemiology and investigate the distribution of s. mutans in the oral of children with rampant caries. | 2005 | 15804014 |
| the influence of biosurfactants released by s. mitis bms on the adhesion of pioneer strains and cariogenic bacteria. | the influence of streptococcus mitis bms biosurfactants on the adhesion of eight pioneer and four cariogenic oral bacterial strains was, for a first screening, examined in a microtiter plate assay. the adhesion to pellicle-coated wells of three cariogenic strains was inhibited >70% by the biosurfactants, while only one pioneer strain showed >70% reduction. the reduction for the other strains did not exceed 50%. subsequently, adhesion of streptococcus mutans atcc 25175 and streptococcus sobrinus ... | 2004 | 15788225 |
| arbitrarily primed polymerase chain reaction for fingerprinting the genotype identification of mutans streptococci in children with down syndrome. | this study investigated the possible intrafamilial similarity of mutans streptococcal strains in some families with a child with down syndrome using chromosomal dna fingerprinting. the isolates were genotyped using arbitrarily primed polymerase chain reaction with the opa 02 and opa 03 primers. the results showed that five children with down syndrome harbored mutans streptococci genotypes different from those of their mothers. a matching of genotypes was observed within the control pair (mother/ ... | 2005 | 15779663 |
| cranberry high molecular weight constituents promote streptococcus sobrinus desorption from artificial biofilm. | dental biofilm harbouring oral bacteria is highly correlated with the progression of dental diseases. disruption of biofilm formation via anti-adhesion agents is an alternative means to the antibacterial approach. previous studies have shown that high molecular weight non-dialysable material (ndm) derived from cranberry juice inhibits the adhesion of escherichia coli and the coaggregation of a variety of oral bacteria. in addition, it inhibits the formation of glucans and fructans synthesised by ... | 2005 | 15737520 |
| antimicrobial activity of croton cajucara benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. | we have previously demonstrated that a linalool-rich essential oil from croton cajucara benth presents leishmanicidal activity. in the present study, we demonstrate that this essential oil inhibits the growth of reference samples of candida albicans, lactobacillus casei, staphylococcus aureus, streptococcus sobrinus, porphyromonas gingivalis and streptococcus mutans cell suspensions, all of them associated with oral cavity disease. the purified linalool fraction was only inhibitory for c. albica ... | 2005 | 15720570 |
| low salivary iga activity to cell-surface antigens of mutans streptococci related to hla-drb1*04. | mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. these differences are not readily explained, though several factors are believed to influence the colonization. one factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin a (iga). in a previous study, differences in salivary iga reactions to oral streptococci were observed between human leukocyte antigen (hla)-dr4-positi ... | 2005 | 15720566 |
| expression of the gtfi gene from streptococcus sobrinus in streptococcus anginosus using integration-mediated transformation system. | we have constructed a streptococcus anginosus transformant expressing the gtfi gene from streptococcus sobrinus, using a previously developed integration-mediated transformation system to introduce foreign genes onto the oral streptococcal chromosome, and attempted to evaluate the gene expression. in this system, one cloning plasmid and three pacyc184 derivatives, anchor, heterodimer, and integration plasmids were used for the construction of a series of integrants via homologous recombination. ... | 2004 | 15716022 |
| influence of dentin on the effectiveness of antibacterial agents. | the influence of dentin on the effectiveness of three antibacterial agents (triclosan, glutaraldehyde, naocl) on streptococcus mutans, s. sobrinus, and lactobacillus acidophilus was tested using the agar diffusion method with and without bovine dentin discs (200 mum and 500 mum thickness) placed between bacteria and test substances. the effect of 0.3% triclosan on all tester strains (100%) was reduced after passage through 500 mum dentin discs to 0% (l. acidophilus) and to 22% and 28% (s. mutans ... | 2005 | 15671825 |
| initial biofilm formation of streptococcus sobrinus on various orthodontics appliances. | biofilms accumulate on hard and soft surface in the oral cavity. accumulation of biofilms on orthodontic appliance bear scientific and clinical interest. the objection of this study was to examine the formation of dental biofilm by streptococcus sobrinus on different types of orthodontics appliances, using a model consisting of host and bacterial constituents. the adsorption pattern of saliva to the orthodontics appliances was determined by means of gel electrophoresis coupled with computerized ... | 2004 | 15525380 |
| single-molecule imaging of the dynamic interactions between macromolecules. | in recent years, the development of single-molecule detection techniques has allowed the dynamic properties of biomolecules, which are normally obscured in conventional ensemble measurements, to be measured. one of these single-molecule detection techniques allows the measurement of dissociation and association events of individual molecules to be measured. this technique is based on the unique premise that the mobility between molecules that are bound and the mobility between those that are fre ... | 2004 | 15518396 |
| longitudinal study of transmission, diversity, and stability of streptococcus mutans and streptococcus sobrinus genotypes in brazilian nursery children. | the aim of this study was to perform a follow-up evaluation of the streptococcus mutans and streptococcus sobrinus colonization profile of children's oral cavities, which included the pattern of vertical transmission from mother to child, genotypic diversity, and stability of the strains. the subjects were 16 mother-child pairs, who were monitored for 20 months. samples of saliva, tongue dorsum, alveolar ridge mucosa, and dental plaque from the children were collected bimonthly. saliva samples f ... | 2004 | 15472319 |
| clinical outcomes for early childhood caries (ecc): the influence of salivary mutans streptococci levels. | to assess the relationship between clinical outcomes for children treated for early childhood caries (ecc) and salivary mutans streptococci (ms) levels. | 2004 | 15471521 |
| detection of cariogenic bacteria genes by a combination of allele-specific polymerase chain reactions and a novel bioluminescent pyrophosphate assay. | we developed a novel bioluminescent assay for detection of pyrophosphate in polymerase chain reaction (pcr) product. the principle of this method is as follows: pyrophosphate released by pcr is converted to adenosine 5'-triphosphate (atp) by pyruvate phosphate dikinase in the presence of the substrate pyruvate phosphate and the coenzyme adenosine 5'-monophosphate; subsequently, atp concentration is determined by firefly luciferase reaction. the detection limit of pyrophosphate is 1.56 x 10(-15)m ... | 2004 | 15450805 |
| adaptive acid tolerance response of streptococcus sobrinus. | streptococcus mutans and streptococcus sobrinus are the bacteria most commonly associated with human dental caries. a major virulence attribute of these and other cariogenic bacteria is acid tolerance. the acid tolerance mechanisms of s. mutans have begun to be investigated in detail, including the adaptive acid tolerance response (atr), but this is not the case for s. sobrinus. an analysis of the atr of two s. sobrinus strains was conducted with cells grown to steady state in continuous chemost ... | 2004 | 15375118 |
| validation and inter-examiner agreement of mutans streptococci levels in plaque and saliva of 10-year-old children using simple chair-side tests. | since there are few validation studies of chair-side tests of oral mutans streptococci, we compared a commercially available strip test with the conventional laboratory assay. two plaque samples obtained from the mesial surfaces of the upper right and lower left permanent molars of sixty-five 10-year-old children (boys = 38, girls = 27) were cultured and incubated using chair-side site strip tests (dentocult sm, orion diagnostica). two plaque sampling tools, namely dental floss and micro-brush, ... | 2004 | 15370635 |
| composition of the salivary microflora during habitual consumption of fluoridated milk. | the aim was to evaluate the effect of habitual consumption of fluoridated milk on the composition of the salivary microflora. the study group comprised 20 healthy schoolchildren and young adults with a mean age of 13.6 years and the investigation had a randomized double-blind crossover design with a washout period of 1 month. after professional tooth-cleaning at baseline, the subjects were supplied with either fluoridated (250 ml, 5 ppm f) or non-fluoridated milk for one daily intake during a pe ... | 2004 | 15370633 |
| mutans streptococci, lactobacilli in saliva and acidity from organisms in dental plaque: changes after restorative treatment. | the effect of restorative dental treatment was evaluated on mutans streptococci and lactobacilli in saliva, as well as acid production by plaque bacteria. we used semiquantitative culture kits at time points up to 6 months following treatment. changes in eating habits and oral hygiene during the study period were ruled out using a questionnaire. mutans streptococci, lactobacilli, and acidity all had decreased significantly at 1 week after treatment. acidity often was the first variable to return ... | 2004 | 15366622 |
| time of initial acquisition of mutans streptococci by human infants. | the aim of this study was to detect and monitor the acquisition of mutans streptococci (ms) in healthy brazilian children. samples of 4 different sites (saliva, tongue dorsum, dental ridges, and dental plaque, if teeth were present) were collected from 33 edentulous nursery school infants (5.9+/-1.5 month-old), using sterilized swabs, bi-monthly for 24 months. saliva samples from the mothers were collected only once. after inoculation, and incubation typical morphotype colonies, were isolated an ... | 2004 | 15366617 |
| reduction of salivary s. aureus and mutans group streptococci by a preprocedural chlorhexidine rinse and maximal inhibitory dilutions of chlorhexidine and cetylpyridinium. | staphylococcus aureus and mutans group streptococci can cause, among many other diseases, infective endocarditis and postoperative infections. the reduction of the number of these microorganisms in the oral cavity prior to surgical procedures has been related to a decreased incidence of such occurrences. the aim of this study was to evaluate the effect of a single preprocedural rinse with 0.12% chlorhexidine solution (periogard) on the salivary counts of s aureus and mutans group streptococci an ... | 2004 | 15366527 |
| detection of cariogenic bacterial genes by microchip electrophoresis. | allele-specific pcr primers were designed, based on the dextranase (dex) gene, to identify streptococcus mutans and streptococcus sobrinus in dental plaque; subsequently, pcr products were detected via microchip electrophoresis (me). in order to amplify the dex gene fragment of s. mutans and s. sobrinus, the following two pcr methods were established. duplex allele-specific pcr primers were designed on a region of low dna homology; furthermore, 211 and 126-bp fragments were amplified for s. muta ... | 2004 | 15358306 |
| salivary iga to cariogenic bacteria in hiv-positive children and its correlation with caries prevalence and levels of cariogenic microorganisms. | the interrelationship of hiv infection, dental caries and mucosal immune responses remains controversial. in our study population of 40 hiv-infected and 40 healthy control children (ages 2-5 years) there was a significantly higher prevalence of dental caries in hiv-infected children (p<0.05). the extent of caries correlated with the severity of hiv disease. to determine whether the immunosuppression that ensues after hiv infection could contribute to the increased caries prevalence, the concentr ... | 2004 | 15327638 |
| streptococcus mutans genotypes isolated from root and coronal caries. | the aim of the present study was to evaluate the colonization profile and clonal distribution of streptococcus mutans isolated from oral cavities that presented coronal and root caries lesions. the isolation and biochemical identification of mutans streptococci were carried out by using saliva samples, dental plaque, and tissue from the caries lesions. in order to confirm their molecular identity, s. mutans and streptococcus sobrinus were submitted to the pcr method, using specific primers for p ... | 2004 | 15316190 |
| a method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo. | this study was carried out to develop a method for mapping the distribution of cariogenic oral streptococci, streptococcus mutans and streptococcus sobrinus, from the outermost to the innermost plaque. ten consenting subjects were asked to form plaque by abstaining from tooth brushing over 3 days within in situ plaque-generating devices, which were placed on the upper molars. the plaque formed in the devices was separated into 8-10 layered fractions (100 microm thick). genomic dna was extracted ... | 2004 | 15316189 |
| the influence of mutanase and dextranase on the production and structure of glucans synthesized by streptococcal glucosyltransferases. | glucanohydrolases, especially mutanase [alpha-(1-->3) glucanase; ec 3.2.1.59] and dextranase [alpha-(1-->6) glucanase; ec 3.2.1.11], which are present in the biofilm known as dental plaque, may affect the synthesis and structure of glucans formed by glucosyltransferases (gtfs) from sucrose within dental plaque. we examined the production and the structure of glucans synthesized by gtfs b (synthesis of alpha-(1-->3)-linked glucans) or c [synthesis of alpha-(1-->6)- and alpha-(1-->3)-linked glucan ... | 2004 | 15280057 |
| molecular interactions of surface protein peptides of streptococcus gordonii with human salivary components. | oral streptococci play a large role in dental biofilm formation, and several types interact as early colonizers with the enamel salivary pellicle to form the primary biofilm, as well as to incorporate other bacteria on tooth surfaces. interactions of surface molecules of individual streptococci with the salivary pellicle on the tooth surface have an influence on the etiological properties of an oral biofilm. to elucidate the molecular interactions of streptococci with salivary components, bindin ... | 2004 | 15271944 |
| genetic basis for mutacin n and of its relationship to mutacin i. | the mutans streptococci (ms) are a group of 7 species of dental cariesassociated bacteria of which streptococcus mutans and streptococcus sobrinus are the most important in humans. many ms produce bacteriocin-like inhibitory substances (blis), some of which have been characterised as small peptides capable of inhibiting the growth of closely-related species. these peptides have most commonly been referred to as mutacins. s. mutans strains n and ua140 appear to have closely similar blis activitie ... | 2004 | 15232205 |
| hla, salivary iga and mutans streptococci--is there a relation? | the aim of the present studies was to investigate a possible relationship between the human leukocyte antigen (hla) complex, colonization of mutans streptococci and salivary immunoglobulin a (iga) antibodies against mutans streptococcal antigens. in the first study a strong inverse relationship between hla-dr4 and levels of mutans streptococci was observed for a group of renal transplant patients (i). in a group with healthy blood-donors a similar trend was observed (i). this tendency was also s ... | 2004 | 15224545 |
| [immunization with targeted fusion anticaries dna vaccine via intramuscular route:experiment with murine]. | to observe the expression of a targeted fusion anticaries dna vaccine pgja-p in muscular in vivo. to compare the levels of specific antibodies and anticaries efficacy generated by pgja-p and fusion anticaries dna vaccine pglua-p in gnotobiotic rats, and observe the kinetics of antibody responses in balb/c mice. | 2004 | 15200917 |
| effect of a high-molecular-weight component of cranberry on constituents of dental biofilm. | previous studies have shown that high molecular-weight non-dialysable material derived from cranberry juice (ndm) inhibits co-aggregation of a variety of oral bacteria. | 2004 | 15163648 |
| a caries vaccine? the state of the science of immunization against dental caries. | studies performed in numerous laboratories over several decades have demonstrated the feasibility of immunizing experimental rodents or primates with protein antigens derived from streptococcus mutans or streptococcus sobrinus against oral colonization by mutans streptococci and the development of dental caries. protection has been attributed to salivary iga antibodies which can inhibit sucrose-independent or sucrose-dependent mechanisms of streptococcal accumulation on tooth surfaces according ... | 2004 | 15153693 |
| effect of dentin on the antibacterial activity of dentin bonding agents. | dentin bonding agents with antibacterial effects may inhibit secondary caries formation and pulp inflammation by eliminating residual bacteria in and on dentin. therefore, the antibacterial effects of prime & bond nt (pb), prime & bond nt without fluoride (pbnf), gluma comfort bond (gl), abf, xeno cf ii (xe), 2-hydroxyethyl methacrylate (hema), triethylene glycol dimethacrylate (teg-dma), and 0.2% chlorhexidine were tested against streptococcus mutans, s. sobrinus, and lactobacillus acidophilus ... | 2004 | 15107649 |
| salivary iga reactions to cell-surface antigens of oral streptococci. | in the immunoblot technique, using whole bacteria cell extracts as antigens, both intra- and extracellular antigens are detected, which gives a large number of immunoglobulin a (iga) reactions (immunoblot bands) when incubated with saliva. it is important to distinguish which immunoblot bands represent bacterial cell-surface antigens, since these antigens could be involved in adhesion mechanisms and be available for blocking in vivo. | 2004 | 15107071 |
| inhibitory effects of cranberry juice on attachment of oral streptococci and biofilm formation. | cranberry juice is known to inhibit bacterial adhesion. we examined the inhibitory effect of cranberry juice on the adhesion of oral streptococci strains labeled with [3h]-thymidine to saliva-coated hydroxyapatite beads (s-ha). when the bacterial cells were momentarily exposed to cranberry juice, their adherence to s-ha decreased significantly compared with the control (p < 0.01). their hydrophobicity also decreased dependently with the concentration of cranberry juice. we also evaluated the inh ... | 2004 | 15107065 |
| effect of carbohydrate fatty acid esters on streptococcus sobrinus and glucosyltransferase activity. | mutans streptococci are oral bacteria with a key role in the initiation of dental caries, because their glucosyltransferases synthesize polysaccharides from sucrose that allow them to colonize the tooth surface. among the strategies to prevent dental caries that are being investigated are (1) the inhibition of bacterial growth of mutans streptococci or (2) the inhibition of glucosyltransferases involved in polysaccharide formation. pure fatty acid esters of sucrose, maltose and maltotriose were ... | 2004 | 15063188 |
| oral streptococci exhibit diverse susceptibility to human beta-defensin-2: antimicrobial effects of hbd-2 on oral streptococci. | we examined the antimicrobial effects of human beta-defensin-2 (hbd-2) on 17 species of oral streptococci to investigate the involvement of antimicrobial peptide activity in oral microflora development and the clinical use of the antimicrobial peptide for oral microflora control. oral streptococci exhibit diverse levels of susceptibility to human beta-defensin-2 (hbd-2). two major cariogenic bacterial species, streptococcus mutans ( s. mutans) and s. sobrinus, were found to be susceptible to the ... | 2004 | 15057473 |
| therapeutic vaccine against streptococcus sobrinus-induced caries. | streptococcus sobrinus produces a virulence-associated immunomodulatory protein (vip) which suppresses the host-specific immune response and induces the early production of il-10. in this study, we evaluated the effects of therapeutic immunization with this vip on the incidence of caries in s. sobrinus-infected rats. groups of wistar rats were orally infected with s. sobrinus and fed with sucrose-sweetened drinking water ad libitum. five days later, rats were immunized intranasally with active o ... | 2004 | 15044513 |
| influence of resin monomers on growth of oral streptococci. | ethyleneglycol dimethacrylate monomers have been previously reported to stimulate the growth of certain caries-associated bacteria on the basis of turbidity measurements. to elucidate the detail of this effect, we examined the influence of resin monomers on the growth of streptococcus sobrinus and streptococcus sanguis by determination of bacterial numbers (colony-forming units), morphological observation, and chemical analysis. although the absorbance values in the stationary phase of bacterial ... | 2004 | 15044503 |
| molecular characterization of dextranase from streptococcus rattus. | the complete nucleotide sequence of the dextranase gene of streptococcus rattus atcc19645 was determined. an open reading frame of the dextranase gene was 2,760 bp long and encoded a dextranase protein consisting of 920 amino acids with a molecular weight of 100,163 da and an isoelectric point of 4.67. the s. rattus dextranase purified from recombinant escherichia coli cells showed dextran-hydrolyzing activity with optimal ph (5.0) and temperature (40 c) similar to those of dextranases from stre ... | 2004 | 15031528 |
| a high molecular mass cranberry constituent reduces mutans streptococci level in saliva and inhibits in vitro adhesion to hydroxyapatite. | previous investigations showed that a high molecular mass, non-dialyzable material (ndm) from cranberries inhibits the adhesion of a number of bacterial species and prevents the co-aggregation of many oral bacterial pairs. in the present study we determined the effect of mouthwash supplemented with ndm on oral hygiene. following 6 weeks of daily usage of cranberry-containing mouthwash by an experimental group (n = 29), we found that salivary mutans streptococci count as well as the total bacteri ... | 2004 | 15019739 |
| [base composition of dna from fluoride resistant strains of streptococcus mutans and streptococcus sobrinus]. | to evaluate the genotype change of fluoride-resistant strains of s.mutans and s.sobrinus. | 2001 | 14994079 |
| colonization of streptococcus mutans and streptococcus sobrinus genotypes and caries development in children to mothers harboring both species. | the major route of early acquisition of mutans streptococci in humans is a vertical transmission from mother to child. the purpose of this longitudinal study was to examine the acquisition, distribution and persistence of streptococcus mutans and streptococcus sobrinus in children whose mothers harbored both species and to study the caries incidence in relation to colonization of these bacteria. fifteen mother-child pairs were followed during the child's first 7 years. stimulated salivary sample ... | 2004 | 14767165 |
| anti-cariogenic properties of a water-soluble extract from cacao. | the addition of a water-soluble extract from cacao-extracted powder (cepws) to a cariogenic model food, a white chocolate-like diet that contains 35% sucrose, significantly reduced caries scores in spf rats infected with streptococcus sobrinus 6715, compared to control rats fed a white chocolate-like diet. cepws markedly inhibited water-insoluble glucan (wig) synthesis through crude glucosyltransferases (gtfs) from streptococcus sobrinus b13n in vitro. gtf-inhibitor(s) in cepws was prepared thro ... | 2003 | 14730134 |
| multiplex fish analysis of a six-species bacterial biofilm. | established procedures use different and seemingly incompatible experimental protocols for fluorescent in situ hybridization (fish) with gram-negative and gram-positive bacteria. the aim of this study was to develop a procedure, based on fish and confocal laser scanning microscopy (clsm), for the analysis of the spatial organization of in vitro biofilms containing both gram-negative and gram-positive oral bacteria. biofilms composed of the six oral species actinomyces naeslundii, candida albican ... | 2004 | 14706749 |
| identification of nad+ synthetase from streptococcus sobrinus as a b-cell-stimulatory protein. | streptococcus sobrinus, one agent of dental caries, secretes a protein that induces lymphocyte polyclonal activation of the host as a mechanism of immune evasion. we have isolated from culture supernatants of this bacterium a protein with murine b-cell-stimulatory properties and subsequently cloned the relevant gene. it contains an open reading frame of 825 bp encoding a polypeptide with 275 amino acid residues and a molecular mass of 30 kda. the protein displays high sequence homology with nad( ... | 2004 | 14702311 |
| antibacterial activity of polyphenol components in oolong tea extract against streptococcus mutans. | the purpose of the present study was to determine the antibacterial activity of oolong tea extract on oral streptococci, including streptococcus mutans and streptococcus sobrinus, and to identify the response to its components. antibacterial activity was found when the extract was added to s. mutans cells in chemically defined medium but not in complex broth media. further, pretreatment with bovine serum albumin reduced the antibacterial activity. the extract showed antibacterial activity agains ... | 2004 | 14684970 |
| a comparison of the antibacterial efficacies of essential oils against oral pathogens. | cariogenic bacteria and periodontopathic bacteria are present in dental plaque as biofilms. in this study, we investigated the antibacterial effects of essential oils on the following oral bacteria: porphyromonas gingivalis, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, streptococcus mutans, and streptococcus sobrinus. we tested manuka oil, tea tree oil, eucalyptus oil, lavandula oil, and romarinus oil and determined their minimum inhibitory concentration and minimum bactericida ... | 2004 | 14678476 |
| enolase from streptococcus sobrinus is an immunosuppressive protein. | a strategy of streptococcus sobrinus, a major agent of dental caries, to survive and colonize the host consists of the production of a protein that suppresses the specific antibody responses. we have cloned the gene coding for a protein with immunosuppressive activity. it contains an open reading frame of 1302 base pairs encoding a polypeptide with 434 amino acid residues and a molecular mass of 46910 da. the gene product is homologous to enolases from several organisms. the polypeptide was expr ... | 2004 | 14678332 |
| anticaries efficacy of a new dentifrice for hypersensitivity. | to evaluate the expected anticaries efficacy of a new dentifrice containing stannous fluoride as the anticaries agent and potassium nitrate as the antihypersensitivity agent using a series of laboratory and animal studies. | 2003 | 14579872 |
| synergistic inhibitory effect of cationic peptides and antimicrobial agents on the growth of oral streptococci. | although chlorhexidine is one of the most efficacious antimicrobial agents used for the prevention of dental caries, side effects limit its application. the effects of gaegurin 6 (ggn6), an animal-derived cationic peptide, and its derivatives ptp6 and ptp12 on the growth of oral streptococci were investigated to assess the potential of these agents for use in the prevention of dental caries. the minimal inhibitory concentrations of the peptides for inhibition of the growth of oral streptococci ( ... | 2003 | 14571120 |
| quantitative evaluation of bacteria adherent to polyelectrolyte hema-based hydrogels. | the use of adhesive poly(hema)-based hydrogels is standard practice in dental restorative procedures. microorganisms, which potentially can cause oral pathologies, may colonize these polymers. in the present work, bacterial adhesion to polymers prepared with 2-hydroxyethyl methacrylate (hema) and to different molar ratios of 2-acrylamido-2-methylpropane-sulfonic acid (amps) and/or to 2-methacryloyloxyethyl-tri-methyl-ammonium chloride (metac) co-monomers were tested. a colorimetric assay system ... | 2003 | 14517857 |
| activity of human beta-defensin 3 alone or combined with other antimicrobial agents against oral bacteria. | the in vitro activities of human beta-defensin 3 (hbd-3) alone or combined with lysozyme, metronidazole, amoxicillin, and chlorhexidine were investigated with the oral bacteria streptococcus mutans, streptococcus sanguinis, streptococcus sobrinus, lactobacillus acidophilus, actinobacillus actinomycetemcomitans, and porphyromonas gingivalis. hbd-3 showed bactericidal activity against all of the bacterial species tested. the bactericidal effect was enhanced when the peptide was used in combination ... | 2003 | 14506056 |
| longitudinal study of intrafamilial mutans streptococci ribotypes. | to explore the intrafamilial homology and longitudinal stability of colonization by early acquired mutans streptococci, genomic fingerprinting was performed on 254 strains (192 streptococcus mutans and 62 streptococcus sobrinus strains) collected from 16 families (16 mother-child pairs, seven fathers and four siblings). genomic dna was digested by the restriction endonuclease hindiii, followed by gel electrophoresis, southern blotting, and hybridization with a digoxigenin-labeled 16s rdna probe, ... | 2003 | 12974680 |
| development of a 5' nuclease-based real-time pcr assay for quantitative detection of cariogenic dental pathogens streptococcus mutans and streptococcus sobrinus. | a 5' nuclease taqman pcr assay was developed for the quantitative detection of the major cariogenic bacteria streptococcus mutans and streptococcus sobrinus. the absolute and relative numbers of bacteria were measured by this method. this assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation. | 2003 | 12958287 |
| effects of low-concentrated chlorhexidine on growth of streptococcus sobrinus and primary human gingival fibroblasts. | the aim of this study was to investigate the in vitro effects of low concentrations of chlorhexidine (chx) on the proliferation of streptococcus sobrinus (atcc 33478) and primary human gingival fibroblasts (hgf). liquid cultures of bacteria or human gingival fibroblasts were exposed to chx concentrations ranging from 0.07 to 40 microm in microtiter plates at 37 degrees c for 24 h. bacteria or cells grown without chx served as controls. the effects of chx were determined either by measurements of ... | 2003 | 12938021 |
| identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16s ribosomal rna genes. | mutans streptococci are frequently isolated from dental plaque and carious lesions. these bacteria have been identified by conventional methods such as biochemical and serologic tests followed by the isolation of colonies on the mitis-salivarius agar, which are sometimes inconsistent. recently, species-specific polymerase chain reaction (pcr) has been reported to rapidly identify streptococcus mutans and streptococcus sobrinus. however, in the case of identification and classification into sever ... | 2003 | 12930526 |
| detection of streptococcus mutans and streptococcus sobrinus on the permanent first molars of the mosuo people in china. | streptococcus mutans and streptococcus sobrinus are associated with the development of dental caries. the purpose of this study was to differentiate these bacteria by morphology, biochemical characteristics and pcr, and to compare their occurrence with the prevalence of dental caries in the mosuo people. plaque samples were collected from the permanent first molar in 126 mosuo people (83 females, 43 males, aged 25-55 years, average age 36.1 +/- 7.73). dental status was recorded as dmft by who ca ... | 2003 | 12925830 |
| [rapid detection of streptococcus mutans and streptococcus sobrinus in human saliva by nested polymerase chain reaction]. | to establish a simple and rapid method to detect streptococcus mutans and streptococcus sobrinus simultaneously in human saliva. | 2003 | 12887805 |
| remote glucosyltransferase-microparticle vaccine delivery induces protective immunity in the oral cavity. | intranasally administered dental caries vaccines show significant promise for human application. alternate mucosal routes may be required, however, to induce caries-protective salivary iga antibody in children with respiratory diseases. since rectal mucosa contains inductive lymphoid tissue, we hypothesized that the rectal route could be used to induce salivary immunity to mutans streptococcal glucosyltransferase (gtf), resulting in protective immunity to experimental dental caries. we first exp ... | 2003 | 12823800 |
| nested pcr for detection of mutans streptococci in dental plaque. | mutans streptococci such as streptococcus mutans and streptococcus sobrinus have been implicated in human dental caries. in an attempt to develop a rapid and sensitive method for detecting strep. mutans and strep. sobrinus in dental plaque, a nested pcr amplification based on the 16s rrna gene was employed. | 2003 | 12803559 |
| glucan-binding proteins of the oral streptococci. | the synthesis of extracellular glucan is an integral component of the sucrose-dependent colonization of tooth surfaces by species of the mutans streptococci. in investigators' attempts to understand the mechanisms of plaque biofilm development, several glucan-binding proteins (gbps) have been discovered. some of these, the glucosyltransferases, catalyze the synthesis of glucan, whereas others, designated only as glucan-binding proteins, have affinities for different forms of glucan and contribut ... | 2003 | 12764072 |
| classification of oral streptococci by two-dimensional gel electrophoresis with direct activity stain for glycosyltransferases. | thirty eight strains of oral streptococci were divided into six types by two-dimensional gel electrophoresis (2-de) followed by glycosyltransferase (glt) activity stain: type 1, streptococcus mutans; type 2, streptococcus rattus; type 3, streptococcus sobrinus and streptococcus downei; type 4, streptococcus cricetus; type 5, streptococcus salivarius; and type 6, streptococcus sanguis, streptococcus oralis and streptococcus gordonii. in types 1, 2 and 5, two glucosyltransferases synthesizing wate ... | 2003 | 12753469 |
| mutans streptococci strains prevalence before and after cavity preparation during atraumatic restorative treatment. | critics argue that all carious dentine is not removed from the hand-prepared cavity during the atraumatic restorative treatment (art) procedure, and that the caries process is soon resumed. the aim of this study was to determine the effectiveness of art in removing carious tissue, by investigating the numbers of mutans streptococci and lactobacilli, with emphasis on the prevalence of streptococcus mutans and streptococcus sobrinus strains before, and after art treatment of dental caries. two mic ... | 2003 | 12753467 |
| effectiveness of dentine bonding agents against cariogenic bacteria in vitro: a comparison of two methods. | data obtained from studies on the antimicrobial properties of bonding agents are the subject of controversy, probably because of methodological differences. this study compared two commonly used in vitro methods, the disc agar diffusion test and the well agar diffusion test. agar plates were seeded with streptococcus sobrinus, lactobacillus gasseri, or actinomyces naeslundii. for the well diffusion test, wells cut out of the agar were filled with the test material, and for the disc method, discs ... | 2003 | 12753463 |
| a new selective medium for streptococcus mutans and the distribution of s. mutans and s. sobrinus and their serotypes in dental plaque. | a new selective medium (ms-mut) was developed for the isolation of streptococcus mutans from clinical specimens. the average growth recovery of s. mutans on ms-mut medium was 72.4% of that on ms medium. growth of streptococcus sobrinus was significantly inhibited on the medium with an average recovery of 0.034%. in 103 subjects, s. mutans was detected at 58.3, 75.0 and 95.7% in the dental plaque of caries-free (cf), caries-inactive (ci) and caries-active (ca) subjects, respectively. s. sobrinus ... | 2003 | 12740546 |
| the paradigm shift in the etiology, prevention, and management of dental caries: its effect on the practice of clinical dentistry. | treatment of dental caries as an infectious disease will require a paradigm shift in the way dentists and other health care professionals approach prevention and management of the disease. prevention of dental caries has relied upon patient cooperation and often requires significant lifestyle changes that are at best difficult to implement and maintain or at worst ignored. this paradigm shift in the etiology, prevention, and treatment of dental caries demands that clinicians redirect their energ ... | 2003 | 12693823 |
| effect of a novel type of propolis and its chemical fractions on glucosyltransferases and on growth and adherence of mutans streptococci. | flavonoids have been considered the main biologically active components in propolis. however, a new variety of flavonoid-free propolis was recently found and chemically classified as type 6. because it showed activity against oral microorganisms, this study evaluated the effects of the crude ethanolic extract of this propolis and its chemical fractions on the activity of purified glucosyltransferases (gtfs) and on the growth and adherence of mutans streptococci. the inhibitory effect of propolis ... | 2003 | 12673037 |
| kuwanon g: an antibacterial agent from the root bark of morus alba against oral pathogens. | kuwanon g was isolated from the ethyl acetate fraction of methanol extract of morus alba and its structure was elucidated by 13c-nmr, 1h-nmr and fab-ms. antibacterial activity of kuwanon g was investigated by the minimum inhibitory concentration (mic) test and the viable cell count method. mic of kuwanon g against streptococcus mutans causing dental caries was determined to be 8.0 microg/ml. the bactericidal test showed that kuwanon g completely inactivated s. mutans at the concentration 20 micr ... | 2003 | 12648813 |
| mass transport of macromolecules within an in vitro model of supragingival plaque. | the aim of this study was to examine the diffusion of macromolecules through an in vitro biofilm model of supragingival plaque. polyspecies biofilms containing actinomyces naeslundii, fusobacterium nucleatum, streptococcus oralis, streptococcus sobrinus, veillonella dispar, and candida albicans were formed on sintered hydroxyapatite disks and then incubated at room temperature for defined periods with fluorescent markers with molecular weights ranging from 3,000 to 900,000. subsequent examinatio ... | 2003 | 12620862 |
| [a study of tarnish induced by streptococcus sobrinus serotype g on co-cr, ni-cr and casting titanium alloys]. | the objective of this study was to investigate the tarnish induced by streptococcus sobrinus serotype g on co-cr, ni-cr and casting titanium alloys. | 2002 | 12593192 |
| the effect of polyol-combinant saliva stimulants on s. mutans levels in plaque and saliva of patients with mental retardation. | the effect of chewable saliva-stimulants on streptococcus mutans levels in dental plaque and paraffin-stimulated whole saliva among participants who were mentally disabled was investigated. over 64-days, 98 participants chewed one of four saliva-stimulating tablets five times/day. the tablets contained one of the following: xylitol (x) or sorbitol (s), or 1:1 mixtures of xylitol and erythritol (xe) or sorbitol and erythritol (se). consumption of xylitol and sorbitol in groups x and s was 5.4 gra ... | 2002 | 12580357 |
| [the isolation and identification of pathogenic bacteria from rampant caries in children]. | in order to prevent rampant caries in children, the predominant pathogenic bacteria of rampant caries in children were defined. | 2001 | 12539724 |