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sizing of bdellovibrio during growth.a technique for the counting and relative sizing of host-independent bdellovibrio during growth, with the aid of a modified coulter counter, is described.19751100613
ribonucleic acid destruction and synthesis during intraperiplasmic growth of bdellovibrio bacteriovorus.during growth of bdellovibrio bacteriovorus on (2-14c)uracil-labeled escherichia coli approximately 50% of the radioactivity is incorporated by the bdellovibrio and most of the remainder is released as free nucleic acid bases. kinetic studies showed that 50 and 30s ribosomal particles and 23 and 16s ribosomal ribonucleic acid (rna) of e. coli are almost completely degraded by the first 90 min in a 210- to 240-min bdellovibrio developmental cycle. synthesis of bdellovibrio ribosomal rna was first ...19751097411
application of the deoxyribonucleic acid/ribonucleic acid hybridization technique in bdellovibrio as a model for studying ribonucleic acid turnover in host-parasite systems.the kinetics of host ribonucleic acid (rna) degradation and its resynthesis into bdellovibrio-specific polyribonucleotides has been studied. the kinetics of rna turnover was followed during a one-step synchronous growth cycle of bdellovibrio growing within 32-po4-labeled escherichia coli host cells. the species of labeled rna present at any given time was ascertained through the specificity of the deoxyribonuclei acid (dna)/rna hybridization technique. at nearsaturating levels of rna and at zero ...19751096825
membrane damage and incorporation of escherichia coli components into bdellovibrio bacteriovorus.cytoplasmic membrane of e. coli is degraded within 20 min following infection with bdellovibrio. 50% of cellular 42-k is lost during the first 10 min. the cytoplasmic membrane, 20 min after infection, centrifugated on a sucrose gradient produces a wide band containing the main enzyme activities (succinic dehydrogenase and lactic dehydrogenase) bound to the membrane. the incorporation into bdellovibrio of labelled host cell constituents during intracellular growth has been studied at successive i ...19751096504
energy efficiency of intraperiplasmic growth of bdellovibrio bacteriovorus.the y-atp (energy efficiency) of intraperiplasmic growth of bdellovibrio bacteriovorus was determined from the distribution of radioactivity of the substrate organism ([u-14c]escherichia coli) btween co2 and bdellovibrio cells at the end of growth. a "best" y-atp value of 18.5 was obtained from single growth cycle experiments and an average value of 25.9 from multicycle experiments. both values are much higher than the usual value of 10.5 for bacteria growing in rich media. the bases for the unu ...19751090596
incorporation of long-chain fatty acids of the substrate organism by bdellovibrio bacteriovorus during intraperiplasmic growth.data are presented showing that a large proportion of the fatty acids of bdellovibrio bacteriovorus grown intraperiplasmically are derived unaltered from the fatty acids of its substrate organism. those fatty acids of the bdellovibrio not homologous with those of the substrate organism are derived mainly by metabolic alteration of preexisting fatty acids in the latter. de novo synthesis from acetate occurs only to a small extent. these characteristics of bdellovibrio physiology are in part respo ...19751090595
utilization of nucleoside monophosphates per se for intraperiplasmic growth of bdellovibrio bacteriovorus.during growth of bdellovibrio bacteriovorus on escherichia coli, there was a marked preferential use of e. coli phosphorus over exogenous orthophosphate even though the latter permeated into the intraperiplasmic space where the bdellovibrio was growing. this preferential use occurred to an equal extent for lipid phosphorus and nucleic acid phosphorus. exogenous thymidine-5'-monophosphate competed effectively with [3h]thymine residues of e. coli as a precursor for bdellovibrio deoxyribonucleic ac ...19751090594
effects of methotrexate on intraperiplasmic and axenic growth of bdellovibrio bacteriovorus.the intraperiplasmic growth rate and cell yield of wild-type bdellovibrio bacteriovorus 109j, growing on escherichia coli of normal composition as the substrate, were not markedly inhibited by 10-3 m methotrexate (4-amino-n10-methylpteroylglutamic acid). in contrast, the growth rate and cell yield of the mutant 109ja, growing axenically in 0.5% yeast extract +0.15% peptone, were strongly inhibited by 10-4 and 10-3 m methotrexate. thymine, thymidine, and thymidine-5'-monophosphate, in increasing ...19751090593
isolation of a small rod with lytic activity against vibrio parahaemolyticus from fresh sea water.a small rod, capable of formine crater-like plaques on lawns of vibrio parahaemolyticus, was isolated from a marine environment. the isolate was a gram-negative straight rod with round ends and was small in size, equal to that of halophilic bdellovibrio strain 5501. the isolate appeared to have close taxonomic relationships to cytophaga, since this bacterium moved slowly in a gliding manner on a solid agar surface, hydrolyzed agar and starch, contained yellow pigment and was halophilic. the isol ...19761018344
[ii. the process of spontaneous bacteriolysis in water and micropredator bacteria].in her conference presented in public health school in ankara in june 1975, the author summarizes the present knowledge on spontaneous bacteriolysis process in water and gives information on the bacteria which are thought to be mostly responsible for auto-purification of water, with special emphasis on bdellovibrio bacteriovorus strains.1976933897
effect of light on bdellovibrio bacteriovorus.bdellovibrio underwent photooxidation by visible light in the presence of exogenous photosensitizer and by near-ultraviolet light (325 to 400 nm) in its absence. the colorless, host-dependent wild type was more sensitive to the lethal effect of light than was its pigmented, facultative parasitic mutant. the latter's ability to form colonies was much more sensitive to light than was its plaque-forming capability. the biosynthesis of the mutant pigment was inhibited by diphenylamine, though this i ...1977885837
ultrastructural changes during encystment and germination of bdellovibrio sp.under proper conditions, bdellovibrio sp. strain w cells develop into bdellocysts in appropriate prey bacteria. after attachment and penetration of the prey cell, the encysting bdellovibrio began to accumulate inclusion material and increase in size, and was surrounded by an outer layer of amorphous electrondense material. the cytoplasm of the encysting cell appeared more electron dense, and nuclear areas appeared more compact. during germination of bdellocysts, the outer wall was uniformly brok ...1977873889
characterization of bdellocysts of bdellovibrio sp.bdellovibrio sp. strain w will infect and produce resting cells, termed bdellocysts, in a variety of gram-negative bacteria. bdellocysts appeared to be produced only within susceptible prey and never in their absence. optimum conditions for encystment included infection of stationary-phase prey cells in 0.05 m potassium phosphate buffer (ph 7.5) at concentrations of prey and bdellovibrios of 2 x 10(9) cells per ml with a multiplicity of infection of unity. bdellocysts contained more deoxyribonuc ...1977873888
[electron microscopic study of the flagellae of bdellovibrio bacteriovorus]. 1977859458
[periodic changes of populations in a prey-predator system: escherichia coli-bdellovibrio bacteriovorus].the prey-predator system escherichia coli-bdellovibrio bacterivorus was investigated in a very poor medium. optical density recording and numerations of the bacterial species showed synchronous oscillations of the concentration of both microbial populations.1976820469
alterations in the cell wall of spirillum serpens vhl early in its association with bdellovibrio bacteriovorus 109d.in both freeze-etched and critical-point dried preparations examined by transmission and scanning electron microscopy, respectively, the outer surfaces of the cells of spirillum serpens vhl assume a wrinkled appearance 10-15 min after challenge by bdellovibrion bacteriovorus 109d. this wrinkling effect is believed (on circumstantial evidence) to be caused by the bdellovibrio's disruption of the cell wall lipoprotein of the spirillum. with the exception of those topological changes caused by wrin ...1976818972
response of neisseria gonorrhoeae to bdellovibrio species.bdellovibrio species are small, highly motile bacteria that are predators upon other bacteria in nature. bdellovibrios attach to, penetrate, replicate within, and destroy prey that share the general characteristic of gram negativity. the lipopolysaccharide moiety of the cell membrane of target microorganisms appears to contain the principal receptor site for bdellovibrio attachment. since gonococci also contain lipopolysaccharide that is similar in many respects to that contained within gram-neg ...1976814101
[purity criteria for bdellovibrio bacteriovorus cultures].two-component cultures of bdellovibrio bacteriovorus, a bacterial parasite, are not always pure; sometimes they contain microbial forms, different from the host and parasite, which cannot be isolated by conventional techniques of inoculation on solid growth media. the only way to isolate them is to apply techniques used for the reversion of l-forms of bacteria. the isolated microorganisms have been identified. the criteria of purity were established for two-component cultures consisting of the h ...1975809647
[characteristics of spheroplast formation in the bacterial host in the process of interacting with bdellovibrio bacteriovorus]. 1975806155
interaction between bdellovibrio bacteriovorus and the cytoplasmic membrane of escherichia coli b.adsorption of bdellovibrio bacteriovours (bdv) on the surface of escherichia coli is accompanied by a sharp decrease in the initial rate of entry of alpha-methylglucoside-c-14 and thiomethlgalactopyranoside-c-14 into the host cell. interaction between the parasite and e. coli leads to the rapid departure of previously accumulated labeled glucosides and beta-galactosides from the bacteria. meanwhile the atpcontent in e. coli falls sharply. adsorption bdv e.coli spheroplasts was established as a f ...1975803849
[intracellular membrane structures of bdellovibrio bacteriovorus during development in the bacterial host cell].electron microscope investigation of intracellular membrane structures of bd. bacteriovorous during its intracellular growth and development has been carried out. increased amount of membrane structures has been stated. along with simply organized invaginations of plasmalemma, complicated membrane structures resembling mesosomes of grampositive bacteria are observed. localization of these structures testifies to their involvement in the synthesis of exotoxins and exoenzymes.1976797074
[detection of bdellovibrios and bacteriophages in the seawater near the shores].the frequency of occurrence of bdellovibrios and intestinal bacteriophages at the sites of contamination in the sea and along the sea shore and at the points remote from the sources of contamination was studied. at the contaminated sites bdellovibrios were revealed in 73.3-100% and bacteriophage in 75-83.4% of the samples. there was found to be a moderate correlative association between the bdellovibrios, bacteriophages and the coli titre. in the water of the shore region of the sea bdellovibrio ...1976795231
structure, synthesis, and post-transcriptional modification of ribosomal ribonucleic acid in bdellovibrio bacteriovorus.the structure, synthesis, and post-transcriptional modifications of 23-s and 16-s ribosomal rnas (rrnas) have been studied in the facultatively parasitic bacterium, bdellovibrio bacteriovorus. the mature 23-s and 16-s type of rrnas in bdellovibrio are larger than the analogous molecules in escherichia coli by at least 1.0 - 10(5) and 0.5 - 10(5) daltons, respectively, and have a conformation different from e. coli rrnas as judged by relative electrophoretic mobilities in polyacrylamide gels with ...1976791372
relationship of bdellovibrio elongation and fission to host cell size.the extent of bdellovibrio growth, and hence progeny produced in infected cells, appears to depend upon host cell size as determined from the ratio of ultimitate length of bdellovibrio to host cell area calculated from light microscopy.1976789349
bdellovibrio as symbiont; the associations of bdellovibrios with other bacteria interpreted in terms of a generalized scheme for classifying organismic associations. 1975785671
effect of virginiamycin on the growth cycle of bdellovibrio.the two components of virginiamycin, virginiamycin m (vm) and virginiamycin s (vs), were used to explore the life cycle of symbiosis-dependent and -independent strains of bdellovibrio bacteriovorus during multiplication in a two-membered system with either living or heat-inactivated escherichia coli or in axenic cultures. relatively high concentrations of these inhibitors separately were required to stop growth under all the conditions, but the minimum inhibitory concentration of the single comp ...1976769670
[detection of bdellovibrio bacteriovorus bacteria in the body of animals]. 1979761809
penicillin-induced formation of osmotically stable spheroplasts in nongrowing bdellovibrio bacteriovorus.bdellovibrio peptidoglycan is of typical gram-negative composition. the molar ratios of alanine:glutamic acid:diaminopimelic acid:muramic acid:glucosamine were about 2:1:1:1:1. nascent, nongrowing bdellovibrio bacteriovorus 109j were converted from highly motile vibrios to highly motile spheres when shaken in dilute buffer plus penicillin, cephalothin, bacitracin, or d-cycloserine. the spherical forms contained essentially no sedimentable peptidoglycan; i.e., they were spheroplasts. spheroplasts ...1978641013
characterization of germination and activation of bdellovibrio bdellocysts.a simple method of assaying germination of bdellocysts in liquid medium has been devised. bdellocysts can be induced to germinate by any of eight l-amino acids or the monovalent cations k+ and nh4+. l-glutamine was the best individual inducer of germination, although the resulting rate of germination was much slower than in a complex medium. the use of a defined germination medium containing l-glutamine, kcl, and nh4cl produced a faster rate of germination than did complex media. bdellocysts ger ...1978618835
chemotaxis of bdellovibrio bacteriovorus toward pure compounds.positive chemotaxis by bdellovibrio bacteriovorus strain uki2 was measured for 139 compounds. twenty-one compounds were attractants; sensitive attraction was elicited by acetate, propionate, thioacetate, malonate, cis-oxalacetate, d-glucose-6-phosphate, acetyl coenzyme a, ammonium ion, barium ion, manganous ion, and potassium ion. several of the attractants for b. bacteriovorus strain uki2 also were attractants to strains 6-5-s and 114; however, strains 109d and 109j were not attracted by the co ...1979500565
[interaction between bdellovibrio bacteriovorus and cholera vibrios in sewage]. 1979499847
[intracellular parasitism of bacteria]. 1979459961
[ultrastructural characteristics of bdellovibrio chlorellavorus].intracellular and extracellular polar lamellar systems were detected in bdellovibrio chlorellavorus, the obligate parasite of chlorella. they were observed in negatively contrasted preparations and thin sections. these systems are considered as an essential character of the bacterium, being connected with its parasitic behaviour.1979423805
chemotaxis by bdellovibrio bacteriovorus toward prey.a chemotaxis assay system that uses a modified boyden chamber was characterized and used for measurements of chemotaxis by bdellovibrio bacteriovorus strain uki2 toward several bacterial species. bacteria tested included both susceptible and nonsusceptible cells (escherichia coli, pseudomonas fluorescens, bacillus megaterium, and b. bacteriovorus strains uki2 and d). none was attractive to bdellovibrios when present at densities below 10(7) cells per ml. chemotaxis toward e. coli was studied mos ...1977410796
elongation and cell division in bdellovibrio bacteriovorus.elongation and division of bdellovibrio bacteriovorus were studied in axenic synchronous cultures. the cells elongate unidirectionally from one end attaining a length of several "unit cells", and then divide into the corresponding number of cells. the length of the filament and, consequently, the progeny number, vary within the range of two to several dozen cells, according to the conditions used. a protein and a low molecular weight component are required for normal division.1977410384
[several features of the antigenic structure of bdellovibrio bacteriovorus].a method of growing bdellovibrio bacteriovorus (bdv) based on the use of the host microbe in the state of reduced vital activity permitted to obtain bdv cultures which could be used for the preparation specific antisera. immunochemical analysis of 4 bdv strains showed them to possess individual antigenic components localized in the alpha 2-globulin zone. testing 17 bdv strains isolated from natural water bodies showed 11 of them to form precipitation lines with the antisera to 4 bdv strains.1977409009
regulated breakdown of escherichia coli deoxyribonucleic acid during intraperiplasmic growth of bdellovibrio bacteriovorus 109j.during growth of bdellovibrio bacteriovorus on [2-14c]deoxythymidine-labeled escherichia coli, approximately 30% of the radioactivity was released to the culture fluid as nucleoside monophosphates and free bases; the remainder was incorporated by the bdellovibrio. by 60 min after bdellovibrio attack, when only 10% of the e. coli deoxyribonucleic acid (dna) had been solubilized, the substrate cell dna was degraded to 5 x 10(5)-dalton fragments retained within the bdelloplast. kinetic studies show ...1979387743
[biological characteristics of bdellovibrio bacteriovorus]. 1979377864
some problems of the ecology and taxonomy of marine microvibrios.the bactericidal effect of water from the indian and pacific oceans and caspian and white seas on escherichia coli was studied. it is shown that a decrease in the viability of e. coli cells is accompanied by the appearance and active multiplication of small bacteria of a vibrioid form. two strains of such bacteria were isolated from a suspension of e. coli in seawater and purified from membranes of the substrate organism by the method of centrifugation in a ficoll gradient. the marine bacteria o ...1978375993
[dynamics of the interaction of bdellovibrio bacteriovorus with the bacterial host under aerobic and anaerobic conditions].bdellovibrio bacteriovorus can interact with the host bacterium cells under aerobic and anaerobic conditions. the dynamics of the interaction between the parasite and the host depended on the regime of incubation. the latent period and the time at which bd. bacteriovorus reached the stationary level became shorter under aerobic conditions. lysis of the host bacterium cells under aerobic conditions proceeded more effectively, at a higher rate, and after a shorter period of the constant titre than ...1979370513
further taxonomic characterization of the genus bdellovibrio.cultures of bdellovibrio isolated from different geographic locations have been studied in terms of deoxyribonucleic acid analysis (% g + c, genome size, and dna hybridization), cytochrome spectrum, and host range. isolates of the genus exhibit a broad range of % g + c ranging from 37 to 51% and the genome sizes extend from 1300 x 10(6) to 1700 x 10(6) daltons. dna hybridization continues to reveal a high level of genetic heterogeneity. bdellovibrio 3294 exhibits 32% relative reassociation to bd ...1978369668
metabolism of rna-ribose by bdellovibrio bacteriovorus during intraperiplasmic growth on escherichia coli.during intraperiplasmic growth of bdellovibrio bacteriovorus 109j on escherichia coli some 30 to 60% of the initial e. coli rna-ribose disappeared as cell-associated orcinol-positive material. the levels of rna-ribose in the suspending buffer after growth together with the rna-ribose used for bdellovibrio dna synthesis accounted for 50% or less of the missing rna-ribose. with intraperiplasmic growth in the presence of added u-14c-labeled cmp, gmp, or ump, radioactivity was found both in the resp ...1978363699
intraperiplasmic growth of bdellovibrio bacteriovorus 109j: solubilization of escherichia coli peptidoglycan.during penetration of bdellovibrio bacteriovorus into escherchia coli, two enzymatic activities, a glycanase and a peptidase, rapidly solubilized some 10 to 15% of the e. coli peptidoglycan. the glycanase activity, which solubilizes peptidoglycan amino sugars, came to a sharp halt with completion of the penetration process. peptidase activity, which cleaves diaminopimelic acid residues from the peptidoglycan, continued, but at a decreasing rate. by 90 min after bdellovibrio attack, some 30% of t ...1978357428
intraperiplasmic growth of bdellovibrio bacteriovorus 109j: attachment of long-chain fatty acids to escherichia coli peptidoglycan.during the initial stages of intraperiplasmic growth of bdellovibrio bacteriovorus on escherichia coli, the peptidoglycan of the e. coli becomes acylated with long-chain fatty acids, primarily palmitic acid (60%) and oleic acid (20%). the attachment of the fatty acids to the peptidoglycan involves a carboxylic-ester bond, i.e., they were removed by treatment with alkaline hydroxylamine. their linkage to the peptidoglycan does not involve a protein molecule. when the bdelloplast peptidoglycan was ...1978357411
intraperiplasmic growth of bdellovibrio bacteriovorus 109j: n-deacetylation of escherichia coli peptidoglycan amino sugars.during intraperiplasmic growth of bdellovibrio bacteriovorus on escherichia coli, the substrate cell peptidoglycan is extensively modified as it is converted to bdelloplast peptidoglycan. the initially lysozyme-sensitive peptidoglycan of e. coli was rapidly converted to a lysozyme-resistant form. the conversion was due to the n-deacetylation of a large portion of the peptidoglycan amino sugars. chemically acetylating the isolated peptidoglycan restored its sensitivity to lysozyme digestion. howe ...1978357410
[fluctuations in the numbers of bdellovibrio bacteriovorus and host bacteria in a 2-component system].the number of interacting organisms in a two-membered bacterial "bdellovibrio bacteriovorus--bacterium-host" system varied within a long period of time. the variations occurred in the counterphase and had a complicated irregular character with an altering period. the volterra principle of collisions can be applied to this type of interaction between microorganisms.1978351340
intraperiplasmic growth of bdellovibrio bacteriovorus on heat-treated escherichia coli.heat treatment (55 degrees c for 40 min) of cell suspensions in buffer (ca. 3 x 10(9) cells per ml) of escherichia coli ml35 caused a 4- to 4.5-log loss of cell viability. similar results were found for several other e. coli strains that were examined. as a result of this heat treatment, 260-nm- and 280-nm-absorbing materials were released into the suspending buffer, along with about 10% of the total cellular radioactivity, when cells uniformly labeled with (14)c were used. in comparison with un ...1978346559
growth of host dependent bdellovibrio in host cell free system.a particulate, subcellular fraction of escherichia coli was shown to promote the growth of host dependent (h-d) bdellovibrio in the absence of host cells. the growth promoting activity was enhanced by both cations and trypisn, and destroyed by pronase. during the axenic growth unipolar spheres appear in the elongating bdellovibrio forms. thymidine monophosphate was more readily incorporated than thymidine into the bdellovibrio dna during growth in the host free system.1978345991
effects of nuclei acid compounds on viability and cell composition of bdellovibrio bacteriovorus during starvation. 1978345990
[purification of bdellovibrio bacteriovorus of the cells and membranes of the bacteria hosts]. 1978342570
bdellovibrio and the intestinal flora of vertebrates.bdellovibrio strain ms7 force-fed to fish and frogs via an intragastric tube did not become an integral component of the intestinal microflora. strain ms7 fed to mice in drinking water was not recovered from the intestinal tract of mice. however, in vitro, the organism multiplied in intestinal contents of frogs and mice. bdellovibrio inoculated into rabbit ileal loops was greatly reduced in number within 24 h. it was concluded that strains ms7 could be considered nonpathogenic to animals, at lea ...1977337896
[effect of temperature on the dynamics of interaction of bdellovibrio bacteriovorus with host bacteria].the dynamics of the interaction between bdellovibrio bacteriovorus and the host bacterium was found to depend on temperature. the maximum rate of infection was found at 37 degrees c. the maximum yield of bdellovibrio and the maximum lysis of the host cells occurred at 22.5 degrees c. the cardinal points, at which no interaction was observed, have been determined. it is concluded that b. bacteriovorus belongs to mesophilis microorganisms.1977333239
osmoregulation in symbiosis-independent mutants of bdellovibrio bacteriovorus.bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with escherichia coli. the axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine.1977327930
[method for culturing bdellovibrio bacteriovorus on an agar gel]. 1977320098
[ultrastructure of micropredators of gram-positive bacilli and the degeneration of c perfringens]. 1976188562
energy metabolism of bdellovibrio bacteriovorus. ii. p/o ratio and atp pool turnover rate.the p/o ratio of bdellovibrio bacteriovorus, strain bd 109 sa, was evaluated by two different methods based on the determination of energy-rich phosphate bonds and either nadh oxidation or oxygen-uptake. p/o values calculated on the basis of nadh oxidation were up to 6, which has to be regarded as being overestimated. p/o values calculated from energy-rich phosphate bonds and oxygen uptake were around 2. the p/o values determined for escherichia coli b were similar. the loss of phosphorylation e ...1976179488
[health aspects of the study of bdellovibrio bacteriovorus as a natural factor in the self-purification of reservoirs]. 1975179245
[distribution of bdellovibrio bacteriovorus parasitizing pseudomonas fluorescens and serratia marcescens in river water]. 1979160878
glycolytic and tricarboxylic acid cycle enzyme activities during intraperiplasmic growth of bdellovibrio bacteriovorus on escherichia coli.selected enzyme activities were measured in extracts of the total cell pellets obtained at various times during aerobic intraperiplasmic growth of bdellovibrio bacteriovorus 109j on anaerobically grown escherichia coli substrate cells. initially, the glycolytic enzyme activities were associated with the input of e. coli and the tricarboxylic acid cycle enzyme activities with the input of bdellovibrios. during the first 90 min of bdellovibrio development, the glycolytic activities declined about ...1976135759
[bdellovibrio bacteriovorus as a factor in the self-purification of river water]. 1976134929
membrane-associated, energy-linked reactions in bdellovibrio bacteriovorus.disrupted cells of bdellovibrio bacteriovorus exhibited adenosine triphosphatase activity, 60 to 80% of which was in the soluble fraction. dicyclohexylcarbodiimide did not inhibit the adenosine triphosphatase activity in membrane particles. the particles did not show energy-linked transhydrogenase activity. the activity of non-energy-linked transhydrogenase as well as the rate of oxygen consumption were higher in membrane particles of the host-independent strain than in the host-dependent strain ...1976134028
[fatty acid composition of marine microvibrios]. 1979111825
[multiplication of bdellovibrio bacteriovorus in the cytoplasm of the bacterial host].the bacterial parasite bdellovibrio bacteriovorus was studied in the process of its interaction with the host bacterium pseudomonas fluorescens. as has been shown by time-lapse microcinematography, along with the normal growth of b. bacteriovorus in the periplasmatic space of the host bacterium, occasionally (4--5%) the parasite is located in the cytoplasm where the complete stage of its intracellular growth takes place with the release of progeny.197897504
[determination of the index of bdellovibrio bacteriovorus in the water of open reservoirs]. 197880485
antigenicity of bdellovibrios.antigenic relationships between 12 locally isolated bdellovibrios and 3 established reference strains (109d, 6-5-s, and uki2) were investigated. antigenicity of the strains was examined by use of the micro-complement fixation test, the serum and complement bactericidal test, and the immunodiffusion test. antisera were prepared against one of the local strains (ms7) and against one of the established reference strains (uki2). the complement fixation titers suggest a close relationship among all s ...197768711
interactions between bdellovibrio and its host cell.the bdellovibrios are extremely small bacteria with the unique property of being parasites of other (gram-negative) bacteria. in the presence of viable and susceptible bacteria a bdellovibrio cell physically 'attacks' an individual host cell, attaches to its surface, penetrates the cell wall, and multiples within the periplasmic (intramural) space of its prey. the invading bdellovibrio and its progeny degrade and consume the cellular constituents of the invaded host bacterium. this process final ...197936623
chemotaxis toward amino acids by bdellovibrio bacteriovorus.chemotaxis toward amino acids by bdellovibrio bacteriovorous strain uki2 was studied by the capillary technique of adler (j. gen. microbiol. 74:77-91, 1973). chemotaxis was shown to be optimal when the capillaries were incubated at between 15 and 40 degrees c for 30 min; the optimal ph was between 7.0 and 8.2. the chemotactic response was proportional to the density of the suspension of bdellovibrios up to a density of 10(8) cells/ml. b. bacteriovorus was attracted to l-asparagine, l-cysteine, l ...197717594
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