Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| nf-kappab activation suppresses host cell apoptosis during rickettsia rickettsii infection via regulatory effects on intracellular localization or levels of apoptogenic and anti-apoptotic proteins. | rickettsia rickettsii, a gram-negative and obligate intracellular bacterium, is the causative agent of rocky mountain spotted fever. in human infections, the primary target of r. rickettsii infection is vascular endothelium. our laboratory has shown that activation of nuclear transcription factor-kappa b (nf-kappab) during r. rickettsii infection of cultured human endothelial cells protects against apoptosis by preventing the activation of apical caspases-8 and -9, and the effector caspase-3. to ... | 2004 | 15135541 |
| rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the united states. | ticks, including many that bite humans, are hosts to several obligate intracellular bacteria in the spotted fever group (sfg) of the genus rickettsia. only rickettsia rickettsii, the agent of rocky mountain spotted fever, has been definitively associated with disease in humans in the united states. herein we describe disease in a human caused by rickettsia parkeri, an sfg rickettsia first identified >60 years ago in gulf coast ticks (amblyomma maculatum) collected from the southern united states ... | 2004 | 14999622 |
| antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens. | the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ... | 2004 | 14765731 |
| fatal spotted fever rickettsiosis, minas gerais, brazil. | the emergence and reemergence of a serious infectious disease are often associated with a high case-fatality rate because of misdiagnosis and inappropriate or delayed treatment. the current reemergence of spotted fever rickettsiosis caused by rickettsia rickettsii in brazil has resulted in a high proportion of fatal cases. we describe two familial clusters of brazilian spotted fever in the state of minas gerais, involving six children 9 months to 15 years of age; five died. immunohistochemical i ... | 2003 | 14718082 |
| rickettsia species infecting amblyomma cooperi ticks from an area in the state of são paulo, brazil, where brazilian spotted fever is endemic. | owing to the potential role of the tick amblyomma cooperi in the enzootic cycle of rickettsia rickettsii, the etiologic agent of brazilian spotted fever (bsf), this study evaluated infection by rickettsia species in a. cooperi ticks collected from an area in brazil where bsf is endemic. among a total of 40 a. cooperi adult ticks collected in an area of bsf endemicity in the state of são paulo, pcr analysis detected dna of rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positiv ... | 2004 | 14715737 |
| spotted-fever-group rickettsioses in north asia. | 2003 | 14667766 | |
| rocky mountain spotted fever as a cause of macular star figure. | an 86-year-old woman with a history of tick bites in the previous months developed subnormal visual acuity in both eyes, keratic precipitates, anterior chamber and vitreous cells, optic disc edema, retinal hemorrhages, and retinal arteriolar sheathing. she had no fever or skin rash. three weeks later, binocular macular star figures appeared. brain imaging was negative; cerebrospinal fluid disclosed a lymphocytic pleocytosis and elevated protein. the serum rickettsia rickettsii antibody test was ... | 2003 | 14663310 |
| evaluation of a pcr assay for quantitation of rickettsia rickettsii and closely related spotted fever group rickettsiae. | a spotted fever rickettsia quantitative pcr assay (sq-pcr) was developed for the detection and enumeration of rickettsia rickettsii and other closely related spotted fever group rickettsiae. the assay is based on fluorescence detection of sybr green dye intercalation in a 154-bp fragment of the rompa gene during amplification by pcr. as few as 5 copies of the rompa gene of r. rickettsii can be detected. sq-pcr is suitable for quantitation of r. rickettsii and 10 other genotypes of spotted fever ... | 2003 | 14662926 |
| implications of presumptive fatal rocky mountain spotted fever in two dogs and their owner. | a dog was examined because of petechiation, an inability to stand, pale mucous membranes, a possible seizure, and thrombocytopenia. tick-borne illness was suspected, but despite treatment, the dog died. eight days later, a second dog owned by the same individual also died. the dog was not examined by a veterinarian, but rocky mountain spotted fever (rmsf) was suspected on the basis of clinical signs. two weeks after the second dog died, the owner was examined because of severe headache, fever, n ... | 2003 | 14627095 |
| isolation of bartonella washoensis from a dog with mitral valve endocarditis. | we report the first documented case of bartonella washoensis bacteremia in a dog with mitral valve endocarditis. b. washoensis was isolated in 1995 from a human patient with cardiac disease. the main reservoir species appears to be ground squirrels (spermophilus beecheyi) in the western united states. based on echocardiographic findings, a diagnosis of infective vegetative valvular mitral endocarditis was made in a spayed 12-year-old female doberman pinscher. a year prior to presentation, the re ... | 2003 | 14605197 |
| mycobacterium marinum escapes from phagosomes and is propelled by actin-based motility. | mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection. numerous intracellular pathogens, including listeria monocytogenes, shigella flexneri, and rickettsia rickettsii, exploit the host cytoskeleton by using actin-based motility for cell to cell spread during infection. here we show that mycobacterium marinum, a natural pathogen of fish and frogs and an occa ... | 2003 | 14597736 |
| [study on spotted fever group rickettsiae in guangdong province]. | to explore the existence of spotted fever group rickettsiae (sfgr) in guangdong province. | 2003 | 14521792 |
| serologically detected "new" tick-borne zoonoses in eastern croatia. | to establish serologically a contact with causative agents of human monocytic and granulocytic ehrlichiosis, human babesiosis, recently detected rickettsioses, and lyme disease in individuals with a history of tick bite from three counties in eastern croatia. | 2003 | 14515426 |
| the rocky mountain spotted fever group of rickettsias. | 1965 | 14318051 | |
| [experimental transmission of rickettsiae to arthropods]. | 1964 | 14249721 | |
| results of a five-year survey for certain enzootic diseases in the fauna of western utah. | 1965 | 14248984 | |
| [sensitivity of the bat artibus lituratus palmarum to rickettsia rickettsii]. | 1963 | 14190216 | |
| studies on infectious diseases in wild animals in utah. v. experimental rocky mountain spotted fever in the coyote, canis latrans lestes merriam. | 1963 | 14170550 | |
| effect of dichlorotetrafluoroethane on the infectivity of viruses and rickettsiae sensitive to trichlorotrifluoroethane. | 1963 | 14131240 | |
| [pullulation of rickettsia ricketsii in rats infected by intraperitoneal injections of blood]. | 1963 | 14101730 | |
| ecology of rocky mountain spotted fever in western montana. i. isolation of rickettsia rickettsii from wild mammals. | 1962 | 14041199 | |
| [experiments on the problem of the transovarian transmission of rickettsia]. | 1962 | 14000181 | |
| cell-wall constituents of rickettsiae and psittacosis-lymphogranuloma organisms. | 1963 | 13942448 | |
| factors affecting the growth of rickettsias of the spotted fever group in fertile hens' eggs. | 1962 | 13917322 | |
| spotted fever vaccine; potency assay by direct challenge of vaccinated mice with toxin of rickettsia rickettsii. | 1961 | 13866804 | |
| the transmission of low virulent strains of rickettsia rickettsii. | 1961 | 13737980 | |
| identification of rickettsia rickettsii in the wood tick, dermacentor andersoni, by means of fluorescent antibody. | 1960 | 13688983 | |
| the effect of red blood cells on experimental infection of white rats with the r strain of rickettsia rickettsii. | 1958 | 13508659 | |
| persistence of rickettsia rickettsii in a patient recovered from rocky mountain spotted fever. | 1954 | 13212060 | |
| the epidemiology of rocky mountain spotted fever. ii. studies on the biological survival mechanism of rickettsia rickettsii. | 1954 | 13207101 | |
| recovery of a rickettsia of the spotted fever group from microtus pennsylvanicus from virginia. | 1954 | 13167137 | |
| the epidemiology of rocky mountain spotted fever. i. the characterization of strain virulence of rickettsia rickettsii. | 1953 | 13080263 | |
| a quantitative analysis of the factors involved in the variations in virulence of rickettsiae. | 1953 | 13076181 | |
| natural infection of the tick, amblyomma cajennense, with rickettsia rickettsii in panama. | 1953 | 13065638 | |
| interference phenomenon in animal infections with rickettsiae of rocky mountain spotted fever. | 1953 | 13037839 | |
| [artificial transmission of rickettsia rickettsii (rocky mountain spotted fever) on insects, especially on body lice]. | 1952 | 13015971 | |
| spotted fever rickettsiosis in coronel fabriciano, minas gerais state. | we report cases of spotted fever rickettsiosis in coronel fabriciano municipality of minas gerais state, brazil. the cases occurred in may and june of 2000. during this period there were two deaths among children from an area named pedreira in a periurban area of this municipality. in a boy who died with clinical manifestations of brazilian spotted fever, a necropsy revealed the presence of a spotted fever group rickettsia. the serological results confirm the difficulty in the differential diagn ... | 2003 | 12937725 |
| cross-protection between distantly related spotted fever group rickettsiae. | two excellent c3h/hen mouse models of spotted fever rickettsioses caused by the distantly related organisms, rickettsia conorii and rickettsia australis, were utilized to evaluate the possibility of the stimulation of broad cross-protective immunity. sublethal infection stimulated complete immunity, that is absence of disease, after challenge with a dose of the heterologous rickettsia that uniformly killed naïve mice. in contrast, heterologous immune sera did not protect mice against a lethal do ... | 2003 | 12922124 |
| the immunologic response of dogs to bartonella vinsonii subspecies berkhoffii antigens: as assessed by western immunoblot analysis. | bartonella vinsonii subspecies berkhoffii is a recently recognized zoonotic pathogen that causes endocarditis, granulomatous rhinitis, and granulomatous lymphadenitis in dogs. isolation of b. vinsonii (berkhoffii) from blood or tissue samples is frequently unsuccessful; therefore, diagnosis is primarily dependent on serologic or molecular testing modalities. because previous canine serologic studies have used an indirect immunofluorescence assay (ifa), without western immunoblot (wi) confirmatio ... | 2003 | 12918816 |
| serologic evidence of infection with ehrlichiae and spotted fever group rickettsiae among residents of gag island, indonesia. | the causative agents of scrub and murine typhus are considered endemic to indonesia. however, the presence of spotted fever group rickettsiae and ehrlichiae have not been previously described in this country. during an investigation of arthropod-borne diseases on gag island, located northwest of the island of new guinea in eastern indonesia, the prevalence of antibody to the etiologic agents of monocytic ehrlichiosis, spotted fever rickettsiosis, and scrub and murine typhus were determined. anal ... | 2003 | 12875301 |
| molecular and functional analysis of the lepb gene, encoding a type i signal peptidase from rickettsia rickettsii and rickettsia typhi. | the type i signal peptidase lepb genes from rickettsia rickettsii and rickettsia typhi, the etiologic agents of rocky mountain spotted fever and murine typhus, respectively, were cloned and characterized. sequence analysis of the cloned lepb genes from r. rickettsii and r. typhi shows open reading frames of 801 and 795 nucleotides, respectively. alignment analysis of the deduced amino acid sequences reveals the presence of highly conserved motifs that are important for the catalytic activity of ... | 2003 | 12867468 |
| genetic analysis of isolates of rickettsia rickettsii that differ in virulence. | we examined the degree of intraspecies genetic polymorphisms present among 13 r. rickettsii strains isolated from the blood of patients and ixodid ticks from north and south america. preliminary confirmation of these isolates as r. rickettsii was done by evaluating rsai and psti restriction fragment length polymorphic (rflp) sites in a 631 bp fragment of the rompa gene amplified with primers encompassing the rr190.70-701 nt region. all isolates had the same profile, which was identical to that p ... | 2003 | 12860712 |
| interactions of rickettsia rickettsii with endothelial nuclear factor-kappab in a "cell-free" system. | 2003 | 12860701 | |
| rickettsial actin-based motility: behavior and involvement of cytoskeletal regulators. | actin-based motility (abm) is employed by spotted fever group (sfg) rickettsiae, such as rickettsia rickettsii, to promote cell-to-cell spread. time-lapse video microscopy revealed that abm is not strictly confined to sfg rickettsiae as typhus group r. typhi moved at approximately the same rate as r. rickettsii (approximately 4 micro m/min), but in a highly erratic fashion. a number of common behaviors were observed between abm of r. typhi and r. rickettsii, such as entrance into plasma membrane ... | 2003 | 12860687 |
| rickettsia rickettsii infection in the pine vole, microtus pinetorum: kinetics of infection and quantitation of antioxidant enzyme gene expression by rt-pcr. | the pine vole, microtus pinetorum, was evaluated as a laboratory animal model for infection with rickettsia rickettsii. voles demonstrated signs of acute disease, and 45% of infected animals died following intraperitoneal infection with 3 x 10(6) plaque forming units of r. rickettsii. spleen, liver, kidney, lung, brain, testes and blood were analyzed for rickettsial burden by a quantitative pcr assay. the distribution of rickettsiae in tissues during the course of infection was determined by imm ... | 2003 | 12860675 |
| physician knowledge of the diagnosis and management of rocky mountain spotted fever: mississippi, 2002. | rocky mountain spotted fever (rmsf), a tick-borne illness that has its highest incidence in the south central and southeastern united states, is often a diagnostic challenge, as patients frequently present with nonspecific symptoms during the early stages of illness. rmsf has a high case fatality rate among untreated individuals, and the median time from onset of symptoms to death is only eight days, making early recognition and treatment of rmsf crucial. in two mississippi public health distric ... | 2003 | 12860642 |
| rickettsialpox in new york city: a persistent urban zoonosis. | rickettsialpox, a spotted fever rickettsiosis, was first identified in new york city (nyc) in 1946. during the next five years, approximately 540 additional cases were identified in nyc. however, during the subsequent five decades, rickettsialpox received relatively little attention from clinicians and public health professionals, and reporting of the disease diminished markedly. during february 2001 through august 2002, 34 cases of rickettsialpox in nyc were confirmed at cdc from cutaneous biop ... | 2003 | 12860597 |
| ecology and epidemiology of spotted fever group rickettsiae and new data from their study in russia and kazakhstan. | rickettsiae represent a wide range of pathogenicity from classic and new pathogens to endosymbionts of eukaryotic cells. recent studies of rickettsiae have widened the number of representatives of genus rickettsia, especially in the spotted fever group (sfg). rickettsiae of sfg are tick-borne microorganisms with effective transovarial and transstadial transmission. the main hosts are ticks (dermacentor, rhipicephalus, haemophysalis, ixodes, and amlyomma). strategy of maintenance of tick microorg ... | 2003 | 12860595 |
| nuclear factor kappa b protects against host cell apoptosis during rickettsia rickettsii infection by inhibiting activation of apical and effector caspases and maintaining mitochondrial integrity. | apoptotic host cell death is a critical determinant in the progression of microbial infections and outcome of resultant diseases. the potentially fatal human infection caused by rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, involves the vascular endothelium of various organ systems of the host. earlier studies have shown that survival of endothelial cells (ec) during this infection depends on their ability to activate the transcription factor nuclear factor kappa b ... | 2003 | 12819104 |
| characterization of the ftsz gene from ehrlichia chaffeensis, anaplasma phagocytophilum, and rickettsia rickettsii, and use as a differential pcr target. | degenerate primers corresponding to highly conserved regions of previously characterized ftsz genes were used to pcr amplify a portion of the ftsz gene from the genomic dna of ehrlichia chaffeensis (ftsz(ech)), anaplasma phagocytophilum (ftsz(ap)), and rickettsia rickettsii (ftsz(rr)). genome walking was then used to amplify the 5' and 3' termini of the genes. the dna sequences of the resulting amplification products yielded open reading frames coding for proteins with molecular masses of 42.0, ... | 2003 | 12804116 |
| antibodies reactive to rickettsia rickettsii among children living in the southeast and south central regions of the united states. | the reported annual incidence of rocky mountain spotted fever in the united states is 2.2 per million, but studies have suggested that human infection with rickettsia rickettsii may be more common. this study estimated the prevalence of antibodies reactive to r rickettsii among children living in the southeastern and south central united states. | 2003 | 12742879 |
| platelet aggregation in dogs experimentally infected with rickettsia rickettsii. | platelet aggregation studies were performed on nine beagle dogs experimentally infected with rickettsia rickettsia. platelets from dogs with rocky mountain spotted fever tended to be more aggregable than controls. | 1990 | 12684943 |
| fort chaffee revisited: the epidemiology of tick-borne rickettsial and ehrlichial diseases at a natural focus. | a retrospective cohort study was conducted among troops training at fort chaffee, arkansas, from may through june 1997, to identify infections caused by tick-borne pathogens. serum samples were tested by ifas for antibodies to selected rickettsia and ehrlichia species and by an investigational eia for spotted fever group rickettsia lipopolysaccharide antigens. of 1,067 guardsmen tested, 162 (15.2%) had antibodies to one or more pathogens. of 93 guardsmen with paired serum samples, 33 seroconvert ... | 2001 | 12653142 |
| urban zoonoses caused by bartonella, coxiella, ehrlichia, and rickettsia species. | the last half of the 20th century witnessed an increase in the occurrence and recognition of urban zoonoses caused by members of the genera bartonella, coxiella, ehrlichia, and rickettsia, all traditionally considered to be members of the family rickettsiaceae. in recent years, new human pathogens (bartonella elizabethae, bartonella henselae, and rickettsia felis) have been recognized in urban environments. other newly recognized pathogens (ehrlichia chaffeensis and ehrlichia phagocytophila in t ... | 2001 | 12653141 |
| brazilian spotted fever: description of a fatal clinical case in the state of rio de janeiro. | we describe a case of brazilian spotted fever in a previously healthy young woman who died with petechial rash associated to acute renal and respiratory insufficiency 12 days following fever, headache, myalgia, and diarrhea. serologic test in a serum sample, using an immunofluorescence assay, revealed reactive igm/igg. | 2002 | 12621675 |
| effects of ectopically expressed neuronal wiskott-aldrich syndrome protein domains on rickettsia rickettsii actin-based motility. | neuronal wiskott-aldrich syndrome protein (n-wasp) and the actin-related protein 2/3 (arp2/3) complex have emerged as critical host proteins that regulate pathogen actin-based motility. actin tail formation and motility in listeria monocytogenes require the arp2/3 complex but bypasses n-wasp signaling. motility of shigella flexneri and vaccinia virus requires both n-wasp and the arp2/3 complex. functional roles for these cytoskeletal regulatory proteins in actin-based motility of rickettsia rick ... | 2003 | 12595475 |
| deep origin of plastid/parasite atp/adp translocases. | membrane proteins that transport atp and adp have been identified in mitochondria, plastids, and obligate intracellular parasites. the mitochondrial atp/adp transporters are derived from a broad-specificity transport family of eukaryotic origin, whereas the origin of the plastid/parasite atp/adp translocase is more elusive. here we present the sequences of five genes coding for atp/adp translocases from four species of rickettsia. the results are consistent with an early duplication and divergen ... | 2003 | 12574860 |
| comparison of pcr assays for detection of the agent of human granulocytic ehrlichiosis, anaplasma phagocytophilum. | human granulocytic ehrlichiosis is an emerging infectious disease in the united states and europe, and pcr methods have been shown to be effective for the diagnosis of acute infections. numerous pcr assays and primer sets have been reported in the literature. the analytical sensitivities (limits of detection) of 13 published pcr primer sets were compared using dna extracted from serial dilutions of anaplasma phagocytophilum-infected hl-60 cells. the specificity of the assays that were able to de ... | 2003 | 12574272 |
| [emerging and reemerging rickettsiosis in an endemic area of minas gerais state, brazil]. | this article describes a serological survey for rickettsiosis in the county of novo cruzeiro, minas gerais state, brazil, in 1998, testing schoolchildren and dogs. sera included 331 samples from schoolchildren from an endemic area and 142 samples from schoolchildren from a non-endemic area in the county. all children examined were healthy and had not reported clinical symptoms of brazilian spotted fever prior to the serological survey. some 35 children in the endemic area were reactive to ricket ... | 2002 | 12488886 |
| phylogenetic analysis of the erythrocytic anaplasma species based on 16s rdna and groel (hsp60) sequences of a. marginale, a. centrale, and a. ovis and the specific detection of a. centrale vaccine strain. | phenotypic criteria for the identification of erythrocytic ruminant anaplasma species has relied on subjective identification methods such as host pathogenicity (virulence for cattle or sheep) and/or the location of anaplasma inclusion bodies within the host's red cells. sequence comparisons of new and available genbank accessions were investigated to elucidate the relationships among these closely related anaplasma species. twenty-one 16s rdna and groel (hsp60) sequences from 13 anaplasma margi ... | 2003 | 12488078 |
| rocky mountain spotted fever. | 2002 | 12458609 | |
| rickettsial, ehrlichial and bartonella infections of the myocardium and pericardium. | myocarditis and pericarditis are uncommon complications of human rickettsial, ehrlichial and bartonella infections. myocardial inflammation usually occurs in the setting of acute disseminated infection. organisms associated with myocarditis include: rickettsia rickettsii, r. conorii, orientia tsutsugamushi, coxiella burnetii, anaplasma phagocytophila (the causative agent of human granulocytic ehrlichiosis) and bartonella henselae. pericarditis has been described in the setting of r. conorii and ... | 2003 | 12456377 |
| fever and rash in a 3-year-old girl: rocky mountain spotted fever. | initial symptoms of rocky mountain spotted fever (rmsf), a tick-borne illness caused by rickettsia rickettsii, are nonspecific and include headache, gastrointestinal disturbances, malaise, and myalgias, followed by fever and rash. the classic triad of fever, rash, and history of tick exposure is uncommon at presentation. clinical manifestations of rmsf range from virtually asymptomatic to severe. because of the potentially fatal outcome of rmsf, presumptive clinical diagnosis and empiric antimic ... | 2002 | 12353892 |
| rickettsia rickettsii: as virulent as ever. | 2002 | 12201574 | |
| short report: concurrent rocky mountain spotted fever in a dog and its owner. | a sequential occurrence of rocky mountain spotted fever (rmsf) in a dog and its owner is described. diagnosis of rmsf in the animal guided subsequent testing for and diagnosis of the same disease in the human patient. previous reports of concurrent rmsf in dogs and their owners are reviewed, and the epidemiologic significance of this occurrence is discussed. | 2002 | 12135293 |
| rickettsia rickettsii infection of cultured human endothelial cells induces heme oxygenase 1 expression. | existing evidence suggests that oxidative insults and antioxidant defense mechanisms play a critical role in the host cell response during infection of endothelial cells by rickettsia rickettsii, the causative agent of rocky mountain spotted fever. heme oxygenase (ho), a rate-limiting enzyme in the pathway for heme catabolism, protects against oxidant damage in a variety of stress situations. here, we report on the expression of the inducible and constitutive ho isozymes, ho-1 and ho-2, during r ... | 2002 | 12117910 |
| endothelial cell apoptosis in sepsis. | to discuss a potential role for endothelial cell apoptosis in the pathogenesis of sepsis. | 2002 | 12004240 |
| rocky mountain spotted fever. | rocky mountain spotted fever (rmsf) is a fulminant tick-borne infection by rickettsia rickettsii. the classical diagnostic triad is fever, headache and rash in a patient with a recent tick bite. with prompt, appropriate antimicrobial therapy, patient outcomes are excellent. however, rmsf is often misdiagnosed, particularly during the initial stages of illness, and as a result, patient outcomes are sometimes suboptimal. | 2002 | 11982306 |
| atypical fulminant rickettsia rickettsii infection (brazilian spotted fever) presenting as septic shock and adult respiratory distress syndrome. | brazilian spotted fever, caused by rickettsia rickettsii, has been increasingly reported in brazil especially in the southeastern states. the severe and fulminant forms of the disease are not unusual but most of the reported fatal cases have shown some typical clinical clue, which leads the attending physician to a correct diagnosis. we report a probable case of atypical fulminant brazilian spotted fever that presented full-blown septic shock associated with adult respiratory distress syndrome ( ... | 2002 | 11980610 |
| rocky mountain spotted fever: a review for the pediatrician. | 2002 | 11905289 | |
| evidence of rodent-associated bartonella and rickettsia infections among intravenous drug users from central and east harlem, new york city. | we tested serum samples collected in 1997 and 1998 from a cohort of 204 injection drug users (idus) recruited from central and east harlem, new york city, new york, for antibodies reactive with seven rickettsial or bartonella spp. antigens. rodent-associated bartonella elizabethae and rickettsia akari were the primary etiologic agents of interest. the testing panel also included bartonella henselae, bartonella quintana, rickettsia prowazekii, rickettsia rickettsii, and rickettsia typhi. the high ... | 2001 | 11791987 |
| immunization with a portion of rickettsial outer membrane protein a stimulates protective immunity against spotted fever rickettsiosis. | two approaches for presentation of a part of the rickettsial outer membrane protein a (ompa) of rickettsia rickettsii, namely (1) recombinant mycobacterium vaccae (rmv) or (2) recombinant dna vaccine, stimulated protective immunity against a lethal challenge with the closely related bacterium, r. conorii, in mice. after primary immunization with rmv and booster immunization with homologous recombinant protein, 67 and 55% of mice were protected against challenge in two experiments. dna vaccinatio ... | 2001 | 11738766 |
| risk of lyme disease: perceptions of residents of a lone star tick-infested community. | lone star ticks (amblyomma americanum) have been suggested as a vector of the agent of lyme disease (borrelia burgdorferi sensu lato) in the usa, based on associations with an infection manifesting mainly as erythema migrans. in laboratory experiments, however, they failed to transmit b. burgdorferi sensu stricto. | 2001 | 11693973 |
| spotted fever in brazil: a seroepidemiological study and description of clinical cases in an endemic area in the state of são paulo. | during 1985-1995, illnesses clinically and epidemiologically compatible with brazilian spotted fever were identified in 17 patients in the county of pedreira, in the state of são paulo, brazil. spotted-fever group rickettsial infection was confirmed by serology and/or immunostaining of tissues in 10 of these patients. immunostaining confirmed infection in a 37-year-old pregnant patient, although rickettsial antigens were not demonstrable in the tissues of the fetus. a serosurvey was conducted in ... | 2001 | 11693878 |
| serologic and molecular evidence of coinfection with multiple vector-borne pathogens in dogs from thailand. | forty-nine dogs from thailand were evaluated for serologic evidence of exposure or polymerase chain reaction (pcr) evidence of infection with vectorborne pathogens, including ehrlichia sp. (ehrlichia canis, ehrlichia chaffeensis, ehrlichia equi, and ehrlichia risticii), bartonella vinsonii subsp. berkhoffi (bvb), spotted fever group (sfg) rickettsiae (rickettsia rickettsii), typhus group (tg) rickettsiae (rickettsia canada, rickettsia prowazekii, and rickettsia typhi), and babesia sp. (babesia c ... | 2001 | 11596732 |
| phylogeny of rickettsia spp. inferred by comparing sequences of 'gene d', which encodes an intracytoplasmic protein. | 'gene d' is the ps120-protein-encoding gene, first described in rickettsia conorii and rickettsia japonica. sequence analysis of a 3030 bp fragment of 'gene d' in 24 representatives of the genus rickettsia was carried out to complete phylogenetic analyses previously inferred by comparison of gene sequences encoding citrate synthase, 17 kda antigen and rompa and rompb. the phylogenetic relationships between rickettsiae were inferred from the comparison of both the gene and the derived protein seq ... | 2001 | 11491333 |
| serological evidence for the continued presence of human rickettsioses in southern india. | rickettsiosis is generally believed to have disappeared from many parts of india. however, the serological testing of 37 residents of southern india who presented with fever of unknown aetiology in 1996-1998 confirmed that spotted fever, epidemic/endemic typhus and scrub typhus continue to occur in southern india. the epidemiology and magnitude of the problem need to be evaluated. | 2001 | 11454249 |
| quantitative analyses of variations in the injury of endothelial cells elicited by 11 isolates of rickettsia rickettsii. | eleven isolates of spotted fever group rickettsiae from the blood of patients or ixodid ticks from north and south america were characterized. all isolates were identified as rickettsia rickettsii using restriction fragment length polymorphism analysis of a 532-bp rompa gene fragment obtained by pcr. the ability of the r. rickettsii isolates to elicit cytopathic effects and parameters of oxidative injury were examined in cultured human ea.hy 926 endothelial cells. cytopathic effects were determi ... | 2001 | 11427428 |
| [ehrlichiosis infection prevalence in spain or cross reactions]. | 2001 | 11333754 | |
| assessing the association between the geographic distribution of deer ticks and seropositivity rates to various tick-transmitted disease organisms in dogs. | to determine whether the geographic distribution of deer ticks (ixodes scapularis) was associated with the distribution of dogs seropositive for various tick-transmitted disease organisms (ie, borrelia burgdorferi, rickettsia rickettsii, the human granulocytic ehrlichiosis [hge] agent, ehrlichia canis, and bartonella vinsonii subsp berkhoffii). | 2001 | 11318358 |
| molecular cloning, sequence and characterization of cjst, a putative protease from rickettsia rickettsii. | the cloning and sequencing of a gene from rickettsia rickettsii which confers haemolytic activity on escherichia coli strain tb1 is described. the open reading frame of the haemolysis-promoting gene, cjst, is 1041 bp and encodes a putative protein with a molecular mass of 33 825 da. cjst has high sequence similarity to several bacterial proteases, particularly type iv signal peptidases. cell lysates from an e. coli clone containing cjst in puc19 (pjon1) exhibited greater protease activity in fun ... | 2001 | 11312615 |
| a serological survey of rural dogs and cats on the southwestern canadian prairie for zoonotic pathogens. | a survey for antibodies against agents of plague, tularemia, and rocky mountain spotted fever (rmsf), and against sin nombre hantavirus (snv), bartonella henselae and b. clarridgeiae was conducted in the summer of 1995 using serum from rural dogs and cats living in the vicinity of four public parks in southeastern alberta and southwestern saskatchewan. antibodies to all pathogens were detected in all survey areas. overall prevalence rates were 0.075 for yersinia pestis, 0.089 for francisella tul ... | 2001 | 11257996 |
| rodent ectoparasites from two locations in northwestern florida. | from feb.-apr. 1999, 19 species of ectoparasitic arthropods (2 sucking lice, 4 fleas, 4 ticks, 2 mesostigmatid mites, 5 chiggers, 2 fur mites) were recovered from 106 rodents belonging to 5 species (cotton mouse, peromyscus gossypinus, n = 64; cotton rat, sigmodon hispidus, n = 23; eastern woodrat, neotoma floridana, n = 9; golden mouse, ochrotomys nuttalli, n = 9; eastern gray squirrel, sciurus carolinensis, n = 1) at tall timbers research station, leon county, florida. during the same period, ... | 2000 | 11217221 |
| canine rocky mountain spotted fever: a retrospective study of 30 cases. | rocky mountain spotted fever (rmsf) was diagnosed in 30 dogs examined at north carolina state university, veterinary teaching hospital between 1984 and 1997. historical, physical examination, and laboratory abnormalities were reviewed. diagnostic criteria included a four-fold rise in antibody titer to rickettsia rickettsii (r. rickettsii) (n=15) or a single r. rickettsii antibody titer of 1:1,024 or greater (n=15; when this initial titer was determined one week or more after the onset of clinica ... | 2001 | 11204476 |
| effect of antipyretic therapy on the duration of illness in experimental influenza a, shigella sonnei, and rickettsia rickettsii infections. | to determine whether antipyretic therapy prolongs the course of experimental influenza a, shigella sonnei, and rickettsia rickettsii infections. | 2000 | 11130213 |
| consequences of delayed diagnosis of rocky mountain spotted fever in children--west virginia, michigan, tennessee, and oklahoma, may-july 2000. | patients with rocky mountain spotted fever (rmsf), a tickborne infection caused by rickettsia rickettsii, respond quickly to tetracycline-class antibiotics (e.g., doxycycline) when therapy is started within the first few days of illness; however, untreated rmsf may result in severe illness and death. persons aged <10 years have the highest age-specific incidence of rmsf. this report summarizes the clinical course and outcome of rmsf in four children from four regions of the united states and und ... | 2000 | 11055741 |
| ultrastructure of rickettsia rickettsii actin tails and localization of cytoskeletal proteins. | actin-based motility (abm) is a mechanism for intercellular spread that is utilized by vaccinia virus and the invasive bacteria within the genera rickettsia, listeria, and shigella. within the rickettsia, abm is confined to members of the spotted fever group (sfg), such as rickettsia rickettsii, the agent of rocky mountain spotted fever. infection by each agent induces the polymerization of host cell actin to form the typical f (filamentous)-actin comet tail. assembly of the actin tail propels t ... | 2000 | 10899876 |
| report on ticks collected in the southeast and mid-west regions of brazil: analyzing the potential transmission of tick-borne pathogens to man. | specimens of ticks were collected in 1993, 1996, 1997, and 1998, mostly from wild and domestic animals in the southeast and mid-west regions of brazil. nine species of amblyommidae were identified: anocentor nitens, amblyomma cajennense, amblyomma ovale, amblyomma fulvum, amblyomma striatum, amblyomma rotundatum, boophilus microplus, boophilus annulatus, and rhipicephalus sanguineus. the potential of these tick species as transmitters of pathogens to man was analyzed. a flaviviridade flavivirus ... | 1999 | 10881097 |
| [isolation of rickettsia in vero cell culture]. | the diagnosis of spotted fever is based on characteristic signs and symptoms but requires laboratorial confirmation because of the possible differential diagnosis from other diseases like leptospirosis, enterovirus, meningococcemia and typhoid fever. laboratorial confirmation may be done by detection of specific antibodies which is possible only 5-10 days after the onset of the symptoms or by the isolation of rickettsiae from blood and/or skin biopsy and from ticks collected in the patient or in ... | 1999 | 10881078 |
| meningoencephalitis secondary to bacterial otitis media/interna in a dog. | central nervous system (cns) complications of bacterial otitis media/interna are an infrequent occurrence in human patients and have rarely been reported in the veterinary literature. early recognition of cns involvement and the use of appropriate diagnostic tests to characterize the nature of the lesion(s) are crucial in determining the best course of treatment. in this paper, the authors describe a dog with bacterial meningoencephalitis secondary to otitis media/interna. | 2000 | 10825096 |
| family cluster of rocky mountain spotted fever. | soon after a patient from tennessee died of rocky mountain spotted fever (rmsf), several family members developed symptoms suggestive of the disease and were treated presumptively for rmsf. fifty-four persons visiting the index patient's home were interviewed; serum samples were collected from 35. three additional cases of rmsf were confirmed, all of which occurred in first-degree relatives. time spent at the family home and going into the surrounding woods were significantly associated with dev ... | 1999 | 10825050 |
| in vitro activities of telithromycin (hmr 3647) against rickettsia rickettsii, rickettsia conorii, rickettsia africae, rickettsia typhi, rickettsia prowazekii, coxiella burnetii, bartonella henselae, bartonella quintana, bartonella bacilliformis, and ehrlichia chaffeensis. | in vitro activities of telithromycin compared to those of erythromycin against rickettsia spp., bartonella spp., coxiella burnetii, and ehrlichia chaffeensis were determined. telithromycin was more active than erythromycin against rickettsia, bartonella, and coxiella burnetii, with mics of 0.5 microg/ml, 0.003 to 0.015 microg/ml, and 1 microg/ml, respectively, but was inactive against ehrlichia chaffeensis. | 2000 | 10770788 |
| q fever endocarditis associated with extensive serological cross-reactivity. | 2000 | 10722459 | |
| in vitro susceptibilities of rickettsia and bartonella spp. to 14-hydroxy-clarithromycin as determined by immunofluorescent antibody analysis of infected vero cell monolayers. | the in vitro susceptibilities of rickettsia akari, rickettsia conorii, rickettsia prowazekii, rickettsia rickettsii, bartonella elizabethae, bartonella henselae and bartonella quintana to different concentrations of clarithromycin, 14-hydroxy-clarithromycin (the primary metabolite of clarithromycin) and tetracycline in vero cell cultures, were determined by enumeration of immunofluorescently-stained bacilli. the extent of antibiotic-induced inhibition of foci was recorded for each dilution of an ... | 2000 | 10702548 |
| post-transcriptional regulation of endothelial cell plasminogen activator inhibitor-1 expression during r. rickettsii infection. | intracellular infection of endothelial cells with rickettsia rickettsii results in increased steady-state levels of plasminogen activator inhibitor-1 (pai-1) mrna. control mechanisms governing such increased expression in response to this novel stimulus have not been defined. in this study, we compared the stability of pai-1 mrna in infected and uninfected endothelial cells (ec) and explored the requirement for de novo host cell protein synthesis in the infection-induced increase of steady-state ... | 2000 | 10702354 |
| involvement of protein kinase c in rickettsia rickettsii-induced transcriptional activation of the host endothelial cell. | our laboratory has reported on a biphasic pattern of nuclear factor kappab (nf-kappab) activation in cultured human umbilical vein endothelial cells during infection with rickettsia rickettsii, an obligate, intracellular bacterium, and the etiologic agent of rocky mountain spotted fever. transcriptional activation of the tissue factor (tf) gene during this infection has been shown to involve nf-kappab. to further understand the signal transduction events underlying these phenomena, we studied th ... | 1999 | 10569758 |
| serologic study of the prevalence of rickettsiosis in yucatán: evidence for a prevalent spotted fever group rickettsiosis. | because of the discovery of a spotted fever group rickettsiosis with signs and symptoms similar to dengue fever in yucatan, mexico, immunofluorescence assay (ifa) serology was performed on sera from 390 persons selected from a representative geographic distribution of rural yucatan to detect antibodies reactive with rickettsia rickettsii, r. akari, a thai strain (tt-118) that is most closely related to a rickettsia identified in amblyomma cajennense ticks in southern texas, and r. typhi. the ifa ... | 1999 | 10497980 |
| evidence of rickettsial spotted fever and ehrlichial infections in a subtropical territory of jujuy, argentina. | between november 1993 and march 1994, a cluster 6 pediatric patients with acute febrile illnesses associated with rashes was identified in jujuy province, argentina. immunohistochemical staining of tissues confirmed spotted fever group rickettsial infection in a patient with fatal disease, and testing of serum of a patient convalescing from the illness by using an indirect immunofluorescence assay (ifa) demonstrated antibodies reactive with spotted fever group rickettsiae. a serosurvey was condu ... | 1999 | 10463693 |
| characterization of monoclonal antibodies to an immunodominant protein of the etiologic agent of human granulocytic ehrlichiosis. | immunodominant proteins in the range of 42-45 kd are important for the serodiagnosis of human granulocytic ehrlichiosis (hge). antigens from human isolates of the etiologic agent of hge cultivated in hl-60 cells were used to immunize balb/c mice and generate a panel of hybridomas secreting monoclonal antibodies. using an enzyme immunoassay, an immunofluorescent assay (ifa), and western blotting, we showed that culture supernatants and ascites of these hybridomas were reactive with human isolates ... | 1999 | 10432075 |
| acute disseminated encephalomyelitis after rocky mountain spotted fever. | although acute disseminated encephalomyelitis has been observed after a variety of viral infections and an occasional bacterial infection, it has not been reported in association with rickettsial infections. reported is a 7-year-old male with magnetic resonance images and clinical manifestations suggestive of acute disseminated encephalomyelitis after a tick bite and serologically proven rocky mountain spotted fever. | 1999 | 10428440 |