Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| [bacterial recombinant l-asparaginases: properties, structure and anti-proliferative activity]. | for more than 40 years l-asparaginases are used in combined therapy of acute lymphoblastic leukemia in children and the range of tumors sensitive to these enzymes constantly extends. this review summarizes results of studies aimed at creation of new systems for heterological expression of bacterial l-asparaginases as erwinia carotovora (ewa), helicobacter pylori (hpa), yersinia pseudotuberculosis (ypa) and rhodospirillum rubrum (rra); special attention is paid to isolation of purified enzymes an ... | 2015 | 26215408 |
| genetic, molecular and physiological basis of variation in drosophila gut immunocompetence. | gut immunocompetence involves immune, stress and regenerative processes. to investigate the determinants underlying inter-individual variation in gut immunocompetence, we perform enteric infection of 140 drosophila lines with the entomopathogenic bacterium pseudomonas entomophila and observe extensive variation in survival. using genome-wide association analysis, we identify several novel immune modulators. transcriptional profiling further shows that the intestinal molecular state differs betwe ... | 2015 | 26213329 |
| novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium pantoea sp. strain 3.5.1. | phosphorus is an important macronutrient, but its availability in soil is limited. many soil microorganisms improve the bioavailability of phosphate by releasing it from various organic compounds, including phytate. to investigate the diversity of phytate-hydrolyzing bacteria in soil, we sampled soils of various ecological habitats, including forest, private homesteads, large agricultural complexes, and urban landscapes. bacterial isolate pantoea sp. strain 3.5.1 with the highest level of phytas ... | 2015 | 26209662 |
| a single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens. | a novel lysozyme from cauliflower was purified in a single step, for the first time, using sephadex g100 column chromatography. the purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its molecular mass was calculated to be 22.0 kda. the purified lysozyme showed activity between 30 to 60 °c with 40 °c as the optimum temperature for its maximal activity. although the purified lysozyme was functional at ph ranges betwee ... | 2015 | 26208688 |
| pseudomonas syringae effector avirulence protein e localizes to the host plasma membrane and down-regulates the expression of the nonrace-specific disease resistance1/harpin-induced1-like13 gene required for antibacterial immunity in arabidopsis. | many bacterial pathogens of plants and animals deliver effector proteins into host cells to promote infection. elucidation of how pathogen effector proteins function not only is critical for understanding bacterial pathogenesis but also provides a useful tool in discovering the functions of host genes. in this study, we characterized the pseudomonas syringae pv tomato dc3000 effector protein avirulence protein e (avre), the founding member of a widely distributed, yet functionally enigmatic, bac ... | 2015 | 26206852 |
| l-asparaginase from streptomyces griseus niot-vkma29: optimization of process variables using factorial designs and molecular characterization of l-asparaginase gene. | marine actinobacteria are known to be a rich source for novel metabolites with diverse biological activities. in this study, a potential extracellular l-asparaginase was characterised from the streptomyces griseus niot-vkma29. box-behnken based optimization was used to determine the culture medium components to enhance the l-asparaginase production. ph, starch, yeast extract and l-asparagine has a direct correlation for enzyme production with a maximum yield of 56.78 iu ml(-1). a verification ex ... | 2015 | 26206135 |
| tsv-n1: a novel dna algal virus that infects tetraselmis striata. | numbering in excess of 10 million per milliliter of water, it is now undisputed that aquatic viruses are one of the major factors shaping the ecology and evolution of earth's microbial world. nonetheless, environmental viral diversity and roles remain poorly understood. here we report the first thorough characterization of a virus (designated tsv) that infects the coastal marine microalga tetraselmis striata. unlike previously known microalgae-infecting viruses, tsv is a small (60 nm) dna virus, ... | 2015 | 26193304 |
| oxylipins in moss development and defense. | oxylipins are oxygenated fatty acids that participate in plant development and defense against pathogen infection, insects, and wounding. initial oxygenation of substrate fatty acids is mainly catalyzed by lipoxygenases (loxs) and α-dioxygenases but can also take place non-enzymatically by autoxidation or singlet oxygen-dependent reactions. the resulting hydroperoxides are further metabolized by secondary enzymes to produce a large variety of compounds, including the hormone jasmonic acid (ja) a ... | 2015 | 26191067 |
| drosophila anti-nematode and antibacterial immune regulators revealed by rna-seq. | drosophila melanogaster activates a variety of immune responses against microbial infections. however, information on the drosophila immune response to entomopathogenic nematode infections is currently limited. the nematode heterorhabditis bacteriophora is an insect parasite that forms a mutualistic relationship with the gram-negative bacteria photorhabdus luminescens. following infection, the nematodes release the bacteria that quickly multiply within the insect and produce several toxins that ... | 2015 | 26162375 |
| catalytic mechanisms of fe(ii)- and 2-oxoglutarate-dependent oxygenases. | mononuclear non-heme fe(ii)- and 2-oxoglutarate (2og)-dependent oxygenases comprise a large family of enzymes that utilize an fe(iv)-oxo intermediate to initiate diverse oxidative transformations with important biological roles. here, four of the major types of fe(ii)/2og-dependent reactions are detailed: hydroxylation, halogenation, ring formation, and desaturation. in addition, an atypical epimerization reaction is described. studies identifying several key intermediates in catalysis are conci ... | 2015 | 26152721 |
| two different evolutionary lines of filamentous phages in ralstonia solanacearum: their effects on bacterial virulence. | the integration and excision of various filamentous phage genomes into and out of their host chromosomes occurs by site-specific recombination. the mechanisms proposed for these events include reactions mediated by phage-encoded recombinases and host recombination systems. site-specific integration of filamentous phages plays a vital role in a variety of biological functions of the host, such as phase variation of certain pathogenic bacterial virulence factors. the importance of these filamentou ... | 2015 | 26150828 |
| the regulatory function of lexa is temperature-dependent in the deep-sea bacterium shewanella piezotolerans wp3. | the sos response addresses dna lesions and is conserved in the bacterial domain. the response is governed by the dna binding protein lexa, which has been characterized in model microorganisms such as escherichia coli. however, our understanding of its roles in deep-sea bacteria is limited. here, the influence of lexa on the phenotype and gene transcription of shewanella piezotolerans wp3 (wp3) was investigated by constructing a lexa deletion strain (wp3δlexa), which was compared with the wild-ty ... | 2015 | 26150814 |
| biofumigation on post-harvest diseases of fruits using a new volatile-producing fungus of ceratocystis fimbriata. | a variety of volatile organic compounds (vocs) produced by ceratocystis fimbriata have strong bioactivity against a wide range of fungi, bacteria and oomycetes. mycelial growth, conidial production, and spore germination of fungi and oomycetes were significantly inhibited after exposure to cultures of c. fimbriata, and colony formation of bacteria was also inhibited. two post-harvest diseases, peach brown rot caused by monilinia fructicola and citrus green mold caused by penicillium digitatum, w ... | 2015 | 26147922 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| microbial population dynamics in response to pectobacterium atrosepticum infection in potato tubers. | endophytes are microbes and fungi that live inside plant tissues without damaging the host. herein we examine the dynamic changes in the endophytic bacterial community in potato (solanum tuberosum) tuber in response to pathogenic infection by pectobacterium atrosepticum, which causes soft rot in numerous economically important crops. we quantified community changes using both cultivation and next-generation sequencing of the 16s rrna gene and found that, despite observing significant variability ... | 2015 | 26118792 |
| structural and biochemical characterization of aidc, a quorum-quenching lactonase with atypical selectivity. | quorum-quenching catalysts are of interest for potential application as biochemical tools for interrogating interbacterial communication pathways, as antibiofouling agents, and as anti-infective agents in plants and animals. herein, the structure and function of aidc, an n-acyl-l-homoserine lactone (ahl) lactonase from chryseobacterium, is characterized. steady-state kinetics show that zinc-supplemented aidc is the most efficient wild-type quorum-quenching enzymes characterized to date, with a k ... | 2015 | 26115006 |
| cytoplasmic genome types of european potatoes and their effects on complex agronomic traits. | various wild species germplasm has been used in european potato breeding since the first introduction of potato (solanum tuberosum l.) to europe. as the plant cytoplasmic genome including chloroplast and mitochondrial genomes is transmitted only through the maternal parent, cytoplasmic markers are useful tools in breeding programs to determine cytoplasmic genome types and to trace maternal ancestors. the potato cytoplasmic genome can be distinguished into six distinct types (m, p, a, w, t, and d ... | 2015 | 26112802 |
| application of zinc chloride precipitation method for rapid isolation and concentration of infectious pectobacterium spp. and dickeya spp. lytic bacteriophages from surface water and plant and soil extracts. | this is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against pectobacterium spp. and dickeya spp. from environmental samples. the isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. the method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of zncl2, resuscitation of b ... | 2015 | 26099750 |
| application of zinc chloride precipitation method for rapid isolation and concentration of infectious pectobacterium spp. and dickeya spp. lytic bacteriophages from surface water and plant and soil extracts. | this is the first report describing precipitation of bacteriophage particles with zinc chloride as a method of choice to isolate infectious lytic bacteriophages against pectobacterium spp. and dickeya spp. from environmental samples. the isolated bacteriophages are ready to use to study various (ecological) aspects of bacteria-bacteriophage interactions. the method comprises the well-known precipitation of phages from aqueous extracts of the test material by addition of zncl2, resuscitation of b ... | 2015 | 26099750 |
| endophytic bacterial diversity in the phyllosphere of amazon paullinia cupana associated with asymptomatic and symptomatic anthracnose. | endophytes colonize an ecological niche similar to that of phytopathogens, which make them candidate for disease suppression. anthracnose is a disease caused by colletotrichum spp., a phytopathogen that can infect guarana (paullinia cupana), an important commercial crop in the brazilian amazon. we investigated the diversity of endophytic bacteria inhabiting the phyllosphere of asymptomatic and symptomatic anthracnose guarana plants. the pcr-denaturation gradient gel electrophoresis (pcr-dgge) fi ... | 2015 | 26090305 |
| taking advantage of electric field induced bacterial aggregation for the study of interactions between bacteria and macromolecules by capillary electrophoresis. | the quantification of interaction stoichiometry and binding constant between bacteria (or other microorganism) and (macro)molecules remains a challenging issue for which only a few adapted methods are available. in this paper, a new methodology was developed for the determination of the interaction stoichiometry and binding constant between bacteria and (macro)molecules. the originality of this work is to take advantage of the bacterial aggregation phenomenon to directly quantify the free ligand ... | 2015 | 26086209 |
| a three-protein charge zipper stabilizes a complex modulating bacterial gene silencing. | the hha/ymoa nucleoid-associated proteins help selectively silence horizontally acquired genetic material, including pathogenicity and antibiotic resistance genes and their maintenance in the absence of selective pressure. members of the hha family contribute to gene silencing by binding to the n-terminal dimerization domain of h-ns and modifying its selectivity. hha-like proteins and the h-ns n-terminal domain are unusually rich in charged residues, and their interaction is mostly electrostatic ... | 2015 | 26085102 |
| negative regulation of aba signaling by wrky33 is critical for arabidopsis immunity towards botrytis cinerea 2100. | the arabidopsis mutant wrky33 is highly susceptible to botrytis cinerea. we identified >1680 botrytis-induced wrky33 binding sites associated with 1576 arabidopsis genes. transcriptional profiling defined 318 functional direct target genes at 14 hr post inoculation. comparative analyses revealed that wrky33 possesses dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. we confirmed known wrky33 targets involved in hormone signaling and phy ... | 2015 | 26076231 |
| synergisms between microbial pathogens in plant disease complexes: a growing trend. | plant diseases are often thought to be caused by one species or even by a specific strain. microbes in nature, however, mostly occur as part of complex communities and this has been noted since the time of van leeuwenhoek. interestingly, most laboratory studies focus on single microbial strains grown in pure culture; we were therefore unaware of possible interspecies and/or inter-kingdom interactions of pathogenic microbes in the wild. in human and animal infections, it is now being recognized t ... | 2015 | 26074945 |
| bacillus oryzicola sp. nov., an endophytic bacterium isolated from the roots of rice with antimicrobial, plant growth promoting, and systemic resistance inducing activities in rice. | biological control of major rice diseases has been attempted in several rice-growing countries in asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. two novel endophytic bacillus species, designated strains yc7007 and yc7010(t), with anti-microbial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at ji ... | 2015 | 26060434 |
| validation and application of a real-time pcr protocol for the specific detection and quantification of clavibacter michiganensis subsp. sepedonicus in potato. | clavibacter michiganensis subsp. sepedonicus (cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. although several pcr assays have been developed for detection, they cannot afford specific detection of cms. therefore, in this study, a computational genome analysis was performed to compare the sequenced gen ... | 2015 | 26060431 |
| virulence factors of erwinia amylovora: a review. | erwinia amylovora, a gram negative bacteria of the enterobacteriaceae family, is the causal agent of fire blight, a devastating plant disease affecting a wide range of host species within rosaceae and a major global threat to commercial apple and pear production. among the limited number of control options currently available, prophylactic application of antibiotics during the bloom period appears the most effective. pathogen cells enter plants through the nectarthodes of flowers and other natur ... | 2015 | 26057748 |
| plant expansins: diversity and interactions with plant cell walls. | expansins were discovered two decades ago as cell wall proteins that mediate acid-induced growth by catalyzing loosening of plant cell walls without lysis of wall polymers. in the interim our understanding of expansins has gotten more complex through bioinformatic analysis of expansin distribution and evolution, as well as through expression analysis, dissection of the upstream transcription factors regulating expression, and identification of additional classes of expansin by sequence and struc ... | 2015 | 26057089 |
| functional diversity of jasmonates in rice. | phytohormone jasmonates (ja) play essential roles in plants, such as regulating development and growth, responding to environmental changes, and resisting abiotic and biotic stresses. during signaling, ja interacts, either synergistically or antagonistically, with other hormones, such as salicylic acid (sa), gibberellin (ga), ethylene (et), auxin, brassinosteroid (br), and abscisic acid (aba), to regulate gene expression in regulatory networks, conferring physiological and metabolic adjustments ... | 2015 | 26054241 |
| sfp-type pptase inactivation promotes bacterial biofilm formation and ability to enhance wheat drought tolerance. | paenibacillus polymyxa is a common soil bacterium with broad range of practical applications. an important group of secondary metabolites in p. polymyxa are non-ribosomal peptide and polyketide derived metabolites (nrps/pks). modular non-ribosomal peptide synthetases catalyze main steps in the biosynthesis of the complex secondary metabolites. here we report on the inactivation of an a26 sfp-type 4'-phosphopantetheinyl transferase (sfp-type pptase). the inactivation of the gene resulted in loss ... | 2015 | 26052312 |
| burkholderia cenocepacia lipopolysaccharide modification and flagellin glycosylation affect virulence but not innate immune recognition in plants. | burkholderia cenocepacia causes opportunistic infections in plants, insects, animals, and humans, suggesting that "virulence" depends on the host and its innate susceptibility to infection. we hypothesized that modifications in key bacterial molecules recognized by the innate immune system modulate host responses to b. cenocepacia. indeed, modification of lipopolysaccharide (lps) with 4-amino-4-deoxy-l-arabinose and flagellin glycosylation attenuates b. cenocepacia infection in arabidopsis thali ... | 2015 | 26045541 |
| genome sequence of the potato plant pathogen dickeya dianthicola strain rns04.9. | dickeya dianthicola is one of the causative agents of soft rot and blackleg diseases, which are currently identified in european countries in a wide range of crops. here, we report the draft genome sequence of d. dianthicola strain rns04.9, which was isolated from a potato plant with blackleg symptoms in 2004. | 2015 | 26044429 |
| ethylene response factor 96 positively regulates arabidopsis resistance to necrotrophic pathogens by direct binding to gcc elements of jasmonate - and ethylene-responsive defence genes. | the erf (ethylene responsive factor) family is composed of transcription factors (tfs) that are critical for appropriate arabidopsis thaliana responses to biotic and abiotic stresses. here we identified and characterized a member of the erf tf group ix, namely erf96, that when overexpressed enhances arabidopsis resistance to necrotrophic pathogens such as the fungus botrytis cinerea and the bacterium pectobacterium carotovorum. erf96 is jasmonate (ja) and ethylene (et) responsive and erf96 trans ... | 2015 | 26038230 |
| pectoplate: the simultaneous phenotyping of pectin methylesterases, pectinases, and oligogalacturonides in plants during biotic stresses. | degradation of pectin, a major component of plant cell wall, is important for fungal necrotrophs to achieve a successful infection. the activities of pectin methylesterases (pmes) from both plants and pathogens and the degree and pattern of pectin methylesterification are critical for the outcome of plant-pathogen interaction. partial degradation of pectin by pectin degrading enzymes releases oligogalacturonides (ogs), elicitors of plant defense responses. few analytical techniques are available ... | 2015 | 26029230 |
| enhanced arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases. | upon recognition of microbe-associated molecular patterns (mamps) such as the bacterial flagellin (or the derived peptide flg22) by pattern-recognition receptors (prrs) such as the flagellin sensing2 (fls2), plants activate the pattern-triggered immunity (pti) response. the l-type lectin receptor kinase-vi.2 (lecrk-vi.2) is a positive regulator of arabidopsis thaliana pti. cysteine-rich receptor-like kinases (crks) possess two copies of the c-x8-c-x2-c (duf26) motif in their extracellular domain ... | 2015 | 26029224 |
| cyclic lipopeptide biosynthetic genes and products, and inhibitory activity of plant-associated bacillus against phytopathogenic bacteria. | the antibacterial activity against bacterial plant pathogens and its relationships with the presence of the cyclic lipopeptide (clp) biosynthetic genes ituc (iturin), bmyb (bacillomycin), fend (fengycin) and srfaa (surfactin), and their corresponding antimicrobial peptide products have been studied in a collection of 64 strains of bacillus spp. isolated from plant environments. the most frequent antimicrobial peptide (amp) genes were bmyb, srfaa and fend (34-50% of isolates). most isolates (98.4 ... | 2015 | 26024374 |
| antimicrobial action of an endophytic fungi from sophor flavescens and structure identification of its active constituent. | endophytic fungus bs002 was isolated and characterized from sophora flavescens by plate method, which has broad antimicrobial activity. isolation and trace of a new bioactive compound from the fungus' culture extracts with the method of column chromatography and tlc biological autoradiography was conducted. finally, it was identified as 6,7-(2'e) dibutenyl-5,8-dihydroxy-(z)-cyclooct-2-ene-1,4-dione by nuclear magnetic resonance, infrared and liquid chromatography-mass spectrometry. the compound ... | 2014 | 26019517 |
| regulation of the type i-f crispr-cas system by crp-camp and galm controls spacer acquisition and interference. | the crispr-cas prokaryotic 'adaptive immune systems' represent a sophisticated defence strategy providing bacteria and archaea with protection from invading genetic elements, such as bacteriophages or plasmids. despite intensive research into their mechanism and application, how crispr-cas systems are regulated is less clear, and nothing is known about the regulation of type i-f systems. we used pectobacterium atrosepticum, a gram-negative phytopathogen, to study crispr-cas regulation, since it ... | 2015 | 26007654 |
| cyclic nucleotide gated channel gene family in tomato: genome-wide identification and functional analyses in disease resistance. | the cyclic nucleotide gated channel (cngc) is suggested to be one of the important calcium conducting channels. nevertheless, genome-wide identification and systemic functional analysis of cngc gene family in crop plant species have not yet been conducted. in this study, we performed genome-wide identification of cngc gene family in the economically important crop tomato (solanum lycopersicum l.) and analyzed function of the group ivb slcngc genes in disease resistance. eighteen cngc genes were ... | 2015 | 25999969 |
| effects of plant antimicrobial phenolic compounds on virulence of the genus pectobacterium. | pectobacterium spp. are among the most devastating necrotrophs, attacking more than 50% of angiosperm plant orders. their virulence strategy is based mainly on the secretion of exoenzymes that degrade the cell walls of their hosts, providing nutrients to the bacteria, but conversely, exposing the bacteria to plant defense compounds. in the present study, we screened plant-derived antimicrobial compounds, mainly phenolic acids and polyphenols, for their ability to affect virulence determinants in ... | 2015 | 25981538 |
| experimental approaches to identify small rnas and their diverse roles in bacteria--what we have learnt in one decade of mica research. | nowadays the identification of small rnas (srnas) and characterization of their role within regulatory networks takes a prominent place in deciphering complex bacterial phenotypes. compared to the study of other components of bacterial cells, this is a relatively new but fast-growing research field. although reports on new srnas appear regularly, some srnas are already subject of research for a longer time. one of such srnas is mica, a srna best described for its role in outer membrane remodelin ... | 2015 | 25974745 |
| edin expression in the fat body is required in the defense against parasitic wasps in drosophila melanogaster. | the cellular immune response against parasitoid wasps in drosophila involves the activation, mobilization, proliferation and differentiation of different blood cell types. here, we have assessed the role of edin (elevated during infection) in the immune response against the parasitoid wasp leptopilina boulardi in drosophila melanogaster larvae. the expression of edin was induced within hours after a wasp infection in larval fat bodies. using tissue-specific rnai, we show that edin is an importan ... | 2015 | 25965263 |
| phytase activity in lichens. | phytase activity was investigated in 13 lichen species using a novel assay method. the work tested the hypothesis that phytase is a component of the suite of surface-bound lichen enzymes that hydrolyse simple organic forms of phosphorus (p) and nitrogen (n) deposited onto the thallus surface. hydrolysis of inositol hexaphosphate (insp6 , the substrate for phytase) and appearance of lower-order inositol phosphates (insp5 -insp1 ), the hydrolysis products, were measured by ion chromatography. phyt ... | 2015 | 25963718 |
| xocr, a luxr solo required for virulence in xanthomonas oryzae pv. oryzicola. | xanthomonas oryzae pv. oryzicola (xoc) causes bacterial leaf streak (bls) in rice, a serious bacterial disease of rice in asia and parts of africa. the virulence mechanisms of xoc are not entirely clear and control measures for bls are poorly developed. the solo luxr proteins are widespread and shown to be involved in virulence in some plant associated bacteria (pab). here, we have cloned and characterized a pab luxr solo from xoc, named as xocr. mutation of xocr almost completely impaired the v ... | 2015 | 25932456 |
| plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential. | rhizosphere, the interface between soil and plant roots, is a chemically complex environment which supports the development and growth of diverse microbial communities. the composition of the rhizosphere microbiome is dynamic and controlled by multiple biotic and abiotic factors that include environmental parameters, physiochemical properties of the soil, biological activities of the plants and chemical signals from the plants and bacteria which inhabit the soil adherent to root-system. recent a ... | 2015 | 25926899 |
| recent progress in the use of 'omics technologies in brassicaceous vegetables. | continuing advances in 'omics methodologies and instrumentation is enhancing the understanding of how plants cope with the dynamic nature of their growing environment. 'omics platforms have been only recently extended to cover horticultural crop species. many of the most widely cultivated vegetable crops belong to the genus brassica: these include plants grown for their root (turnip, rutabaga/swede), their swollen stem base (kohlrabi), their leaves (cabbage, kale, pak choi) and their inflorescen ... | 2015 | 25926843 |
| ex situ conservation priorities for the wild relatives of potato (solanum l. section petota). | crop wild relatives have a long history of use in potato breeding, particularly for pest and disease resistance, and are expected to be increasingly used in the search for tolerance to biotic and abiotic stresses. their current and future use in crop improvement depends on their availability in ex situ germplasm collections. as these plants are impacted in the wild by habitat destruction and climate change, actions to ensure their conservation ex situ become ever more urgent. we analyzed the sta ... | 2015 | 25923711 |
| perception of pathogenic or beneficial bacteria and their evasion of host immunity: pattern recognition receptors in the frontline. | plants are continuously monitoring the presence of microorganisms to establish an adapted response. plants commonly use pattern recognition receptors (prrs) to perceive microbe- or pathogen-associated molecular patterns (mamps/pamps) which are microorganism molecular signatures. located at the plant plasma membrane, the prrs are generally receptor-like kinases (rlks) or receptor-like proteins (rlps). mamp detection will lead to the establishment of a plant defense program called mamp-triggered i ... | 2015 | 25904927 |
| comprehensive functional analysis of the 18 vibrio cholerae n16961 toxin-antitoxin systems substantiates their role in stabilizing the superintegron. | the role of chromosomal toxin-antitoxin (ta) systems, which are ubiquitous within the genomes of free-living bacteria, is still debated. we have scanned the vibrio cholerae n16961 genome for class 2 ta genes and identified 18 gene pair candidates. interestingly, all but one are located in the chromosome 2 superintegron (si). the single ta found outside the si is located on chromosome 1 and is related to the well-characterized hipab family, which is known to play a role in antibiotic persistence. ... | 2015 | 25897030 |
| genome mining: prediction of lipopeptides and polyketides from bacillus and related firmicutes. | bacillus and related genera in the bacillales within the firmicutes harbor a variety of secondary metabolite gene clusters encoding polyketide synthases and non-ribosomal peptide synthetases responsible for remarkable diverse number of polyketides (pks) and lipopeptides (lps). these compounds may be utilized for medical and agricultural applications. here, we summarize the knowledge on structural diversity and underlying gene clusters of lps and pks in the bacillales. moreover, we evaluate by us ... | 2015 | 25893081 |
| transcriptional profile of sweet orange in response to chitosan and salicylic acid. | resistance inducers have been used in annual crops as an alternative for disease control. wood perennial fruit trees, such as those of the citrus species, are candidates for treatment with resistance inducers, such as salicylic acid (sa) and chitosan (chi). however, the involved mechanisms in resistance induced by elicitors in citrus are currently few known. | 2015 | 25887907 |
| csra impacts survival of yersinia enterocolitica by affecting a myriad of physiological activities. | a previous study identified a yersinia enterocolitica transposon mutant, gy448, that was unable to export the flagellar type three secretion system (t3ss)-dependent phospholipase, ypla. this strain was also deficient for motility and unable to form colonies on lauria-bertani agar medium. preliminary analysis suggested it carried a mutation in csra. csra in escherichia coli is an rna-binding protein that is involved in specific post-transcriptional regulation of a myriad of physiological activiti ... | 2015 | 25885058 |
| effects of two salts compounds on mycelial growth, sporulation, and spore germination of six isolates of botrytis cinerea in the western north of algeria. | six isolates of botrytis cinerea were isolated from leaves and stems of different tomato varieties taken from four areas in the northwest of algeria where tomato is mostly grown in greenhouses and high tunnels. the purpose of this research was to determine the effect of two salts, nacl and cacl2, on three stages of botrytis cinerea's life cycle. all isolates tested were stimulated in 50 to 150 ppm; nacl was the most effective treatment to increase mycelial growth at two tested concentrations. ho ... | 2015 | 25883657 |
| plant immunity triggered by engineered in vivo release of oligogalacturonides, damage-associated molecular patterns. | oligogalacturonides (ogs) are fragments of pectin that activate plant innate immunity by functioning as damage-associated molecular patterns (damps). we set out to test the hypothesis that ogs are generated in planta by partial inhibition of pathogen-encoded polygalacturonases (pgs). a gene encoding a fungal pg was fused with a gene encoding a plant polygalacturonase-inhibiting protein (pgip) and expressed in transgenic arabidopsis plants. we show that expression of the pgip-pg chimera results i ... | 2015 | 25870275 |
| draft genome sequences for canadian isolates of pectobacterium carotovorum subsp. brasiliense with weak virulence on potato. | pectobacterium carotovurum subsp. brasiliense causes soft rot and blackleg diseases on potato. here, we report the draft genome sequences of three weakly virulent p. carotovurum subsp. brasiliense strains isolated in canada. analysis of these genome sequences will help to pinpoint differences in virulence among p. carotovurum subsp. brasiliense strains from tropical/subtropical and temperate regions, such as canada and united states. a small number of key factors for adaptation to this bacterium ... | 2015 | 25858837 |
| an update on polygalacturonase-inhibiting protein (pgip), a leucine-rich repeat protein that protects crop plants against pathogens. | polygalacturonase inhibiting proteins (pgips) are cell wall proteins that inhibit the pectin-depolymerizing activity of polygalacturonases secreted by microbial pathogens and insects. these ubiquitous inhibitors have a leucine-rich repeat structure that is strongly conserved in monocot and dicot plants. previous reviews have summarized the importance of pgip in plant defense and the structural basis of pg-pgip interaction; here we update the current knowledge about pgips with the recent findings ... | 2015 | 25852708 |
| rpon1, but not rpon2, is required for twitching motility, natural competence, growth on nitrate, and virulence of ralstonia solanacearum. | the plant pathogen ralstonia solanacearum has two genes encoding for the sigma factor σ(54): rpon1, located in the chromosome and rpon2, located in a distinct "megaplasmid" replicon. in this study, individual mutants as well as a double mutant of rpon were created in r. solanacearum strain gmi1000 in order to determine the extent of functional overlap between these two genes. by virulence assay we observed that rpon1 is required for virulence whereas rpon2 is not. in addition rpon1 controls othe ... | 2015 | 25852679 |
| the f-box protein max2 contributes to resistance to bacterial phytopathogens in arabidopsis thaliana. | the arabidopsis thaliana f-box protein more axillary growth2 (max2) has previously been characterized for its role in plant development. max2 appears essential for the perception of the newly characterized phytohormone strigolactone, a negative regulator of polar auxin transport in arabidopsis. | 2015 | 25849639 |
| fusarium oxysporum triggers tissue-specific transcriptional reprogramming in arabidopsis thaliana. | some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including arabidopsis thaliana. to investigate and compare transcriptional changes triggered by f. oxysporum in different arabidopsis tissues, we infected soil-grown pla ... | 2015 | 25849296 |
| the physcomitrella patens unique alpha-dioxygenase participates in both developmental processes and defense responses. | plant α-dioxygenases catalyze the incorporation of molecular oxygen into polyunsaturated fatty acids leading to the formation of oxylipins. in flowering plants, two main groups of α-doxs have been described. while the α-dox1 isoforms are mainly involved in defense responses against microbial infection and herbivores, the α-dox2 isoforms are mostly related to development. to gain insight into the roles played by these enzymes during land plant evolution, we performed biochemical, genetic and mole ... | 2015 | 25848849 |
| characterization of films based on chitosan lactate and its blends with oxidized starch and gelatin. | minimal inhibitory concentration (mic) of chitosan lactate (chl) was tested against bacteria and phytopathogenic fungi. then, the structural, physicochemical and antimicrobial properties of films based on chl, oxidized potato starch (ops), and gelatin (gel) were investigated. with the exception of rhizopus nigricans, chl was effective against the target organisms. gram-positive bacteria (staphylococcus aureus and bacillus cereus) were more sensitive to chl than gram-negative bacteria (pectobacte ... | 2015 | 25841370 |
| regulation of bacterial virulence by csr (rsm) systems. | most bacterial pathogens have the remarkable ability to flourish in the external environment and in specialized host niches. this ability requires their metabolism, physiology, and virulence factors to be responsive to changes in their surroundings. it is no surprise that the underlying genetic circuitry that supports this adaptability is multilayered and exceedingly complex. studies over the past 2 decades have established that the csra/rsma proteins, global regulators of posttranscriptional ge ... | 2015 | 25833324 |
| bacterial expansins and related proteins from the world of microbes. | the discovery of microbial expansins emerged from studies of the mechanism of plant cell growth and the molecular basis of plant cell wall extensibility. expansins are wall-loosening proteins that are universal in the plant kingdom and are also found in a small set of phylogenetically diverse bacteria, fungi, and other organisms, most of which colonize plant surfaces. they loosen plant cell walls without detectable lytic activity. bacterial expansins have attracted considerable attention recentl ... | 2015 | 25833181 |
| [cross-immunogenicity of various bacterial l-asparaginases]. | evaluate immune response in mice against various l-asparaginases and determine their cross-immunogenicity. | 2015 | 25816523 |
| the cost of phage resistance in a plant pathogenic bacterium is context-dependent. | parasites are ubiquitous features of living systems and many parasites severely reduce the fecundity or longevity of their hosts. this parasite-imposed selection on host populations should strongly favor the evolution of host resistance, but hosts typically face a trade-off between investment in reproductive fitness and investment in defense against parasites. the magnitude of such a trade-off is likely to be context-dependent, and accordingly costs that are key in shaping evolution in nature ma ... | 2015 | 25809535 |
| heterologous expression and efficient secretion of chitosanase from microbacterium sp. in escherichia coli. | a recombinant expression vector, pct7-chisp6h, was constructed for the secretory expression of mature peptide of chitosanase (mmschito) from microbacterium sp. ou01. the vector contains several elements, including t7 promoter, signal peptide sequence of mschito, 6 × his-tag sequence and pmaci restriction enzyme cloning site. in pct7-chisp6h, mmschito was fused into signal peptide sequence of mschito gene to construct recombinant plasmid pct7-chisp6h-mmschito. the recombinant plasmid was transfor ... | 2014 | 25805906 |
| heterologous expression and efficient secretion of chitosanase from microbacterium sp. in escherichia coli. | a recombinant expression vector, pct7-chisp6h, was constructed for the secretory expression of mature peptide of chitosanase (mmschito) from microbacterium sp. ou01. the vector contains several elements, including t7 promoter, signal peptide sequence of mschito, 6 × his-tag sequence and pmaci restriction enzyme cloning site. in pct7-chisp6h, mmschito was fused into signal peptide sequence of mschito gene to construct recombinant plasmid pct7-chisp6h-mmschito. the recombinant plasmid was transfor ... | 2014 | 25805906 |
| a novel expansin protein from the white-rot fungus schizophyllum commune. | a novel expansin protein (scexlx1) was found, cloned and expressed from the basidiomycete fungus schizophylum commune. this protein showed the canonical features of plant expansins. scexlx1 showed the ability to form "bubbles" in cotton fibers, reduce the size of avicel particles and enhance reducing sugar liberation from cotton fibers pretreated with the protein and then treated with cellulases. scexlx1 was able to bind cellulose, birchwood xylan and chitin and this property was not affected by ... | 2015 | 25803865 |
| genomic, proteomic and morphological characterization of two novel broad host lytic bacteriophages φpd10.3 and φpd23.1 infecting pectinolytic pectobacterium spp. and dickeya spp. | pectinolytic pectobacterium spp. and dickeya spp. are necrotrophic bacterial pathogens of many important crops, including potato, worldwide. this study reports on the isolation and characterization of broad host lytic bacteriophages able to infect the dominant pectobacterium spp. and dickeya spp. affecting potato in europe viz. pectobacterium carotovorum subsp. carotovorum (pcc), p. wasabiae (pwa) and dickeya solani (dso) with the objective to assess their potential as biological disease control ... | 2015 | 25803051 |
| a cluster of four homologous small rnas modulates c1 metabolism and the pyruvate dehydrogenase complex in rhodobacter sphaeroides under various stress conditions. | in bacteria, regulatory rnas play an important role in the regulation and balancing of many cellular processes and stress responses. among these regulatory rnas, trans-encoded small rnas (srnas) are of particular interest since one srna can lead to the regulation of multiple target mrnas. in the purple bacterium rhodobacter sphaeroides, several srnas are induced by oxidative stress. in this study, we focused on the functional characterization of four homologous srnas that are cotranscribed with ... | 2015 | 25777678 |
| antibacterial and antifungal activity of solvent extracts from plumeria obtusa linn. | extracts of plumeria obtusa are widely used in ethnomedicine and have been investigated for a variety of biological activities; however, the antimicrobial activity of p. obtusa flowers is poorly characterized. in this study, the antimicrobial activities of different solvents (petroleum ether, ethyl acetate, chloroform, isobutanol and ethanol) extracts from flowers of p. obtusa were investigated by a disc diffusion method against gram-positive bacteria, gram-negative bacteria and a fungus. all ex ... | 2014 | 25776586 |
| isolation and genomic characterization of the t4-like bacteriophage pm2 infecting pectobacterium carotovorum subsp. carotovorum. | in order to control pectobacterium carotovorum subsp. carotovorum, a novel virulent bacteriophage pm2 was isolated. bacteriophage pm2 can infect 48% of p. carotovorum subsp. carotovorum and 78% of p. carotovorum subsp. brasilliensis but none of atrosepticum, betavasculorum, odoriferum and wasabiae isolates had been infected with pm2. pm2 phage belongs to the family myoviridae, and contains a large head and contractile tail. it has a 170,286 base pair genome that encodes 291 open reading frames ( ... | 2015 | 25774115 |
| development of pcr and taqman pcr assays to detect pseudomonas coronafaciens, a causal agent of halo blight of oats. | pseudomonas coronafaciens causes halo blight on oats and is a plant quarantine bacterium in many countries, including the republic of korea. using of the certificated seed is important for control of the disease. since effective detection method of p. coronafaciens is not available yet, pcr and taqman pcr assays for specific detection of p. coronafaciens were developed in this study. pcr primers were designed from the draft genome sequence of p. coronafaciens lmg 5060 which was obtained by the n ... | 2015 | 25774107 |
| antimicrobial cyclic peptides for plant disease control. | antimicrobial cyclic peptides derived from microbes bind stably with target sites, have a tolerance to hydrolysis by proteases, and a favorable degradability under field conditions, which make them an attractive proposition for use as agricultural fungicides. antimicrobial cyclic peptides are classified according to the types of bonds within the ring structure; homodetic, heterodetic, and complex cyclic peptides, which in turn reflect diverse physicochemical features. most antimicrobial cyclic p ... | 2015 | 25774105 |
| tropical strains of ralstonia solanacearum outcompete race 3 biovar 2 strains at lowland tropical temperatures. | bacterial wilt, caused by members of the heterogenous ralstonia solanacearum species complex, is an economically important vascular disease affecting many crops. human activity has widely disseminated r. solanacearum strains, increasing their global agricultural impact. however, tropical highland race 3 biovar 2 (r3bv2) strains do not cause disease in tropical lowlands, even though they are virulent at warm temperatures. we tested the hypothesis that differences in temperature adaptation and com ... | 2015 | 25769835 |
| relationship between heat-labile enterotoxin secretion capacity and virulence in wild type porcine-origin enterotoxigenic escherichia coli strains. | heat-labile enterotoxin (lt) is an important virulence factor secreted by some strains of enterotoxigenic escherichia coli (etec). the prototypic human-origin strain h10407 secretes lt via a type ii secretion system (t2ss). we sought to determine the relationship between the capacity to secrete lt and virulence in porcine-origin wild type (wt) etec strains. sixteen wt etec strains isolated from cases of severe diarrheal disease were analyzed by gm1ganglioside enzyme-linked immunosorbent assay to ... | 2015 | 25768732 |
| extracellular production of a novel endo-β-agarase agaa from pseudomonas vesicularis ma103 that cleaves agarose into neoagarotetraose and neoagarohexaose. | the gene agaa, of the isolated marine bacterium pseudomonas vesicularis ma103, comprised 2958-bp nucleotides encoding a putative agarase agaa of 985 amino acids, which was predicted to contain a signal peptide of 29 amino acids in the n-terminus, a catalytic domain of glycoside hydrolase 16 (gh16) family, a bacterial immunoglobulin group 2 (big 2), and three carbohydrate binding modules 6 (cbm 6). the gene agaa was cloned and overexpressed in escherichia coli, and the optimum temperatures for ag ... | 2015 | 25768342 |
| the effect on the transcriptome of anemone coronaria following infection with rust (tranzschelia discolor). | in order to understand plant/pathogen interaction, the transcriptome of uninfected (1s) and infected (2i) plant was sequenced at 3'end by the gs flx 454 platform. de novo assembly of high-quality reads generated 27,231 contigs leaving 37,191 singletons in the 1s and 38,393 in the 2i libraries. estcalc tool suggested that 71% of the transcriptome had been captured, with 99% of the genes present being represented by at least one read. unigene annotation showed that 50.5% of the predicted translati ... | 2015 | 25768012 |
| the broad-spectrum antibiotic, zeamine, kills the nematode worm caenorhabditis elegans. | soil bacteria can be prolific producers of secondary metabolites and other biologically active compounds of economic and clinical importance. these natural products are often synthesized by large multi-enzyme complexes such as polyketide synthases (pkss) or non-ribosomal peptide synthases (nrpss). the plant-associated gram-negative bacterium, serratia plymuthica a153, produces several secondary metabolites and is capable of killing the nematode worm caenorhabditis elegans; a commonly used model ... | 2015 | 25767467 |
| plant secretomics: identification, isolation, and biological significance under environmental stress. | plant secretomes are the proteins secreted by the plant cells and are involved in the maintenance of cell wall structure, relationship between host and pathogen, communication between different cells in the plant, etc. amalgamation of methodologies like bioinformatics, biochemical, and proteomics are used to separate, classify, and outline secretomes by means of harmonizing in planta systems and in vitro suspension cultured cell system (sscs). we summed up and explained the meaning of secretome, ... | 2014 | 25763623 |
| phage display-based generation of novel internalizing antibody fragments for immunotoxin-based treatment of acute myeloid leukemia. | the current standard treatment for acute myeloid leukemia (aml) is chemotherapy based on cytarabine and daunorubicine (7 + 3), but it discriminates poorly between malignant and benign cells. dose-limiting off‑target effects and intrinsic drug resistance result in the inefficient eradication of leukemic blast cells and their survival beyond remission. this minimal residual disease is the major cause of relapse and is responsible for a 5-year survival rate of only 24%. more specific and efficient ... | 2015 | 25760770 |
| deciphering the dual effect of lipopolysaccharides from plant pathogenic pectobacterium. | lipopolysaccharides (lps) are a component of the outer cell surface of almost all gram-negative bacteria and play an essential role for bacterial growth and survival. lipopolysaccharides represent typical microbe-associated molecular pattern (mamp) molecules and have been reported to induce defense-related responses, including the expression of defense genes and the suppression of the hypersensitive response in plants. however, depending on their origin and the challenged plant, lps were shown t ... | 2015 | 25760034 |
| peg-chitosan and glycol-chitosan for improvement of biopharmaceutical properties of recombinant l-asparaginase from erwinia carotovora. | conjugation with the new branched copolymers, peg-chitosan and glycol-chitosan, is suggested to improve the therapeutic properties of l-asparaginase from erwinia carotovora (ewa). the structure and composition of such conjugates were optimized for maximal catalytic efficiency (kcat/km) under physiological conditions, yielding improvement by a factor of 3-6 compared to the native enzyme. this effect is attributed mainly to the shift of ph activity profile towards lower ph values due to the polyca ... | 2015 | 25754046 |
| studies on deimmunization of antileukaemic l-asparaginase to have reduced clinical immunogenicity--an in silico approach. | protein therapeutics, particularly of heterologous origin are shown to elicit immunogenic responses which result in adverse allergic reactions in spite of their promising clinical benefit. l-asparaginase is one such well known chemotherapeutic agent that has enhanced the survival rates to 90 % in the treatment of acute lymphoblastic leukaemia for past 30 years. but the use of this enzyme is accompanied by hypersensitive reactions ranging from allergy to anaphylactic shock which have a drastic in ... | 2015 | 25740072 |
| characterisation of the late blight resistance in potato differential mar9 reveals a qualitative resistance gene, r9a, residing in a cluster of tm-2 (2) homologs on chromosome ix. | the durable late blight resistance in potato plant ma r9 is genetically characterized. a novel r -gene is mapped. the monogenic nature and map positions of r9 are negated and rectified. late blight of potato (solanum tuberosum), caused by phytophthora infestans, can effectively be managed by genetic resistance. the mar9 differential plant provides durable resistance to a broad spectrum of late blight strains. this resistance is brought about by at least seven genes derived from s. demissum inclu ... | 2015 | 25725999 |
| visualization of proteomics data using r and bioconductor. | data visualization plays a key role in high-throughput biology. it is an essential tool for data exploration allowing to shed light on data structure and patterns of interest. visualization is also of paramount importance as a form of communicating data to a broad audience. here, we provided a short overview of the application of the r software to the visualization of proteomics data. we present a summary of r's plotting systems and how they are used to visualize and understand raw and processed ... | 2015 | 25690415 |
| detection, identification and differentiation of pectobacterium and dickeya species causing potato blackleg and tuber soft rot: a review. | the soft rot enterobacteriaceae (sre) pectobacterium and dickeya species (formerly classified as pectinolytic erwinia spp.) cause important diseases on potato and other arable and horticultural crops. they may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the sre as well as in seed c ... | 2014 | 25684775 |
| detection, identification and differentiation of pectobacterium and dickeya species causing potato blackleg and tuber soft rot: a review. | the soft rot enterobacteriaceae (sre) pectobacterium and dickeya species (formerly classified as pectinolytic erwinia spp.) cause important diseases on potato and other arable and horticultural crops. they may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the sre as well as in seed c ... | 2014 | 25684775 |
| absence of lysogeny in wild populations of erwinia amylovora and pantoea agglomerans. | lytic bacteriophages are in development as biological control agents for the prevention of fire blight disease caused by erwinia amylovora. temperate phages should be excluded as biologicals since lysogeny produces the dual risks of host resistance to phage attack and the transduction of virulence determinants between bacteria. the extent of lysogeny was estimated in wild populations of e. amylovora and pantoea agglomerans with real-time polymerase chain reaction primers developed to detect e. a ... | 2015 | 25678125 |
| genomic insights of pectobacterium carotovorum strain m022 quorum-sensing activity through whole-genome sequencing. | pectobacterium carotovorum is known to cause serious damage to various major crops worldwide. here, we report the draft genome of pectobacterium carotovorum strain m022, a freshwater isolate from a malaysian waterfall, which has been reported as a plant pathogen and is able to communicate with n-acylhomoserine lactone-mediated quorum sensing. | 2015 | 25676763 |
| synthetic plant defense elicitors. | to defend themselves against invading pathogens plants utilize a complex regulatory network that coordinates extensive transcriptional and metabolic reprogramming. although many of the key players of this immunity-associated network are known, the details of its topology and dynamics are still poorly understood. as an alternative to forward and reverse genetic studies, chemical genetics-related approaches based on bioactive small molecules have gained substantial popularity in the analysis of bi ... | 2014 | 25674095 |
| synthetic plant defense elicitors. | to defend themselves against invading pathogens plants utilize a complex regulatory network that coordinates extensive transcriptional and metabolic reprogramming. although many of the key players of this immunity-associated network are known, the details of its topology and dynamics are still poorly understood. as an alternative to forward and reverse genetic studies, chemical genetics-related approaches based on bioactive small molecules have gained substantial popularity in the analysis of bi ... | 2014 | 25674095 |
| a metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (cautis). | long-term catheterization inevitably leads to a catheter-associated bacteriuria caused by multispecies bacterial biofilms growing on and in the catheters. the overall goal of the presented study was (1) to unravel bacterial community structure and function of such a uropathogenic biofilm and (2) to elucidate the interplay between bacterial virulence and the human immune system within the urine. to this end, a metaproteomics approach combined with in vitro proteomics analyses was employed to inve ... | 2015 | 25673765 |
| selection of a protein solubilization method suitable for phytopathogenic bacteria: a proteomics approach. | finding the best extraction method of proteins from lysed cells is the key step for detection and identification in all proteomics applications. these are important to complement the knowledge about the mechanisms of interaction between plants and phytopathogens causing major economic losses. to develop an optimized extraction protocol, strains of acidovorax citrulli, pectobacterium carotovorum subsp. carotovorum and ralstonia solanacearum were used as representative cells in the study of phytop ... | 2015 | 25670925 |
| draft genome sequence of a quorum-sensing bacterium, dickeya sp. strain 2b12, isolated from a freshwater lake. | dickeya sp. strain 2b12 was isolated from a freshwater lake in malaysia. here, we report the draft genome sequence of dickeya sp. 2b12 sequenced by the illumina miseq platform. with the genome sequence available, this genome sequence will be useful for the study of quorum-sensing activity in this isolate. | 2015 | 25657288 |
| contribution of diet to the composition of the human gut microbiota. | in the human gut, millions of bacteria contribute to the microbiota, whose composition is specific for every individual. although we are just at the very beginning of understanding the microbiota concept, we already know that the composition of the microbiota has a profound impact on human health. a key factor in determining gut microbiota composition is diet. preliminary evidence suggests that dietary patterns are associated with distinct combinations of bacteria in the intestine, also called e ... | 2015 | 25656825 |
| draft genome sequences of the three pectobacterium-antagonistic bacteria pseudomonas brassicacearum pp1-210f and pa1g7 and bacillus simplex ba2h3. | pectobacterium spp. are bacterial pathogens causing soft rot diseases on a wide range of plants and crops. we present in this paper the draft genome sequences of three bacterial strains, pseudomonas brassicacearum pp1-210f and pa1g7 and bacillus simplex ba2h3, which exhibit antagonistic activities against the pectobacterium plant pathogens. | 2015 | 25635020 |
| endophytic fungi: a reservoir of antibacterials. | multidrug drug resistant bacteria are becoming increasingly problematic particularly in the under developed countries of the world. the most important microorganisms that have seen a geometric rise in numbers are methicillin resistant staphylococcus aureus, vancomycin resistant enterococcus faecium, penicillin resistant streptococcus pneumonia and multiple drug resistant tubercule bacteria to name a just few. new drug scaffolds are essential to tackle this every increasing problem. these scaffol ... | 2014 | 25620957 |
| endophytic fungi: a reservoir of antibacterials. | multidrug drug resistant bacteria are becoming increasingly problematic particularly in the under developed countries of the world. the most important microorganisms that have seen a geometric rise in numbers are methicillin resistant staphylococcus aureus, vancomycin resistant enterococcus faecium, penicillin resistant streptococcus pneumonia and multiple drug resistant tubercule bacteria to name a just few. new drug scaffolds are essential to tackle this every increasing problem. these scaffol ... | 2014 | 25620957 |