Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| aquatic plant azolla as the universal feedstock for biofuel production. | the quest for sustainable production of renewable and cheap biofuels has triggered an intensive search for domestication of the next generation of bioenergy crops. aquatic plants which can rapidly colonize wetlands are attracting attention because of their ability to grow in wastewaters and produce large amounts of biomass. representatives of azolla species are some of the fastest growing plants, producing substantial biomass when growing in contaminated water and natural ecosystems. together wi ... | 2016 | 27777623 |
| significantly enhancing recombinant alkaline amylase production in bacillus subtilis by integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization. | alkaline amylase has significant potential for applications in the textile, paper and detergent industries, however, low yield of which cannot meet the requirement of industrial application. in this work, a novel artp mutagenesis-screening method and fermentation optimization strategies were used to significantly improve the expression level of recombinant alkaline amylase in b. subtilis 168. | 2016 | 27777616 |
| multi-omics analysis on the pathogenicity of enterobacter cloacae enhku01 isolated from sewage outfalls along the ningbo coastline. | the acquisition of iron is important for the pathogenicity of bacteria and blood. three different culture environments (fe stimulation, blood agar plate and normal plate) were used to stimulate enterobacter cloacae, and their respective pathogenicities were compared at the proteomic, mrna and metabolomic levels. | 2016 | 27777513 |
| depth and well type related to groundwater microbiological contamination. | use of groundwater from private wells in households has increased considerably, owing to a better cost/benefit ratio than that of water provided by local utilities for a fee. however, this water is usually untreated, which makes it a vehicle for diseases. thus, monitoring this water is necessary to ensure its integrity and quality. we aimed to evaluate the physical, chemical, and microbiological parameters of untreated groundwater drawn from different types of wells, and the antimicrobial suscep ... | 2016 | 27775681 |
| trojan horse antibiotics-a novel way to circumvent gram-negative bacterial resistance? | antibiotic resistance has been emerged as a major global health problem. in particular, gram-negative species pose a significant clinical challenge as bacteria develop or acquire more resistance mechanisms. often, these bacteria possess multiple resistance mechanisms, thus nullifying most of the major classes of drugs. novel approaches to this issue are urgently required. however, the challenges of developing new agents are immense. introducing novel agents is fraught with hurdles, thus adapting ... | 2016 | 27773991 |
| [increasing incidence of community-acquired pneumonia caused by atypical microorganisms]. | knowing the most common microorganisms in our environment can help us to make proper empirical treatment decisions. the aim is to identify those microorganisms causing community-acquired pneumonia. | 2016 | 27773623 |
| a cluster of kpc-2 and vim-2-producing klebsiella pneumoniae st833 isolates from the pediatric service of a venezuelan hospital. | klebsiella pneumoniae is a bacterial pathogen that has developed resistance to multiple antibiotics and is a major cause of nosocomial infections worldwide. carbapenemase-producing klebsiella pneumoniae have been isolated in many hospitals in venezuela, but they have not been well-studied. the aim of this study was to characterize carbapenem-resistant klebsiella pneumoniae isolates from the pediatric service of a hospital located in anzoategui state, in the eastern part of venezuela. | 2016 | 27770796 |
| bacteriological examination of the abdominal effusion in bacterial peritonitis. | this study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (api20e, api staph, api strep, api ana, biomerieux). rapid tests for detection of oxidase and catalase activity were also used. suscepti ... | 2016 | 27770528 |
| high third-generation cephalosporin resistant enterobacteriaceae prevalence rate among neonatal infections in dakar, senegal. | neonatal infection constitutes one of senegal's most important public health problems, with a mortality rate of 41 deaths per 1,000 live births. | 2016 | 27765017 |
| development of a prediction model for bacteremia in hospitalized adults with cellulitis to aid in the efficient use of blood cultures: a retrospective cohort study. | cellulitis is a common infectious disease. although blood culture is frequently used in the diagnosis and subsequent treatment of cellulitis, it is a contentious diagnostic test. to help clinicians determine which patients should undergo blood culture for the management of cellulitis, a diagnostic scoring system referred to as the bacteremia score of cellulitis was developed. | 2016 | 27756213 |
| application and uses of electronic noses for clinical diagnosis on urine samples: a review. | the electronic nose is able to provide useful information through the analysis of the volatile organic compounds in body fluids, such as exhaled breath, urine and blood. this paper focuses on the review of electronic nose studies and applications in the specific field of medical diagnostics based on the analysis of the gaseous headspace of human urine, in order to provide a broad overview of the state of the art and thus enhance future developments in this field. the research in this field is ra ... | 2016 | 27754437 |
| successful treatment with fecal microbiota transplantation in patients with multiple organ dysfunction syndrome and diarrhea following severe sepsis. | the dysbiosis of intestinal microbiota plays an important role in the development of gut-derived infections, making it a potential therapeutic target against multiple organ dysfunction syndrome (mods) after sepsis. however, the effectiveness of fecal microbiota transplantation (fmt) in treating this disease has been rarely investigated. | 2016 | 27751177 |
| antimicrobial effects of silver nanoparticles stabilized in solution by sodium alginate. | to investigate the effect of nanosilver particles in solution stabilized in a matrix of sodium alginate on the growth and development of pathogenic bacteria such as staphylococcus aureus, enterococcus faecalis, escherichia coli, proteus vulgaris, enterobacter cloacae, the antibiotic-resistant strain of pseudomonas aeruginosa, the yeast-like fungus candida albicans, and the luminescent bacteria photobacterium leiognathi sh1. | 2016 | 27747311 |
| current state of resistance to antibiotics of last-resort in south africa: a review from a public health perspective. | a review of the literature was undertaken to delineate the current level and mechanisms of resistance to carbapenems, colistin, and tigecycline in south africa. thirty-two english publications and 32 national institute of communicable diseases communiqués identified between early january 2000 and 20 may, 2016 showed substantial reports of ndm (n = 860), oxa-48 (n = 584), vim (n = 131), and imp (n = 45) carbapenemases within this period, mainly in klebsiella pneumoniae (n = 1138), acinetobacter b ... | 2016 | 27747206 |
| fortified interpenetrating polymers - bacteria resistant coatings for medical devices. | infections arising from contaminated medical devices are a serious global issue, contributing to antibiotic resistance and imposing significant strain on healthcare systems. since the majority of medical device-associated infections are biofilm related, efforts are being made to generate either bacteria-repellent or antibacterial coatings aimed at preventing bacterial colonisation. here, we utilise a nanocapsule mediated slow release of a natural antimicrobial to improve the performance of a bac ... | 2016 | 27746915 |
| the species accuracy of the most probable number (mpn) european union reference method for enumeration of escherichia coli in marine bivalves. | continuous european union programmes with specified methods for enumeration of escherichia coli in bivalves for human consumption are currently running. the objective of this research was to examine the species accuracy of the five times three tube most probable number (mpn) eu reference method used for detection of e. coli in marine bivalves. among 549 samples of bivalves harvested from norwegian localities during 2014 and 2015, a total number of 200 bacterial isolates were prepared from random ... | 2016 | 27746195 |
| identification of a novel imi carbapenemase variant (imi-9) in enterobacter cloacae complex. | 2016 | 27742202 | |
| a prospective study of the importance of enteric fever as a cause of non-malarial febrile illness in patients admitted to chittagong medical college hospital, bangladesh. | fever is a common cause of hospital admission in bangladesh but causative agents, other than malaria, are not routinely investigated. enteric fever is thought to be common. | 2016 | 27737634 |
| effect of high n-acetylcysteine concentrations on antibiotic activity against a large collection of respiratory pathogens. | the effect of high n-acetylcysteine (nac) concentrations (10 and 50 mm) on antibiotic activity against 40 strains of respiratory pathogens was investigated. nac compromised the activity of carbapenems (of mostly imipenem and, to lesser extents, meropenem and ertapenem) in a dose-dependent fashion. we demonstrated chemical instability of carbapenems in the presence of nac. with other antibiotics, 10 mm nac had no major effects, while 50 mm nac sporadically decreased (ceftriaxone and aminoglycosid ... | 2016 | 27736757 |
| cytotoxicity of methanol extracts of annona muricata, passiflora edulis and nine other cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines. | cancer cells rapidly acquire resistance leading to treatment failures. in the present study, we have evaluated the cytotoxicity of 17 methanol extracts from 11 cameroonian medicinal plants against the sensitive leukemia ccrf-cem cells and the best ones were further tested on a panel of 8 other human cancer cell lines, including various mdr phenotypes as well as against the normal aml12 hepatocytes. | 2016 | 27730025 |
| the diversity of citrus endophytic bacteria and their interactions with xylella fastidiosa and host plants. | the bacterium xylella fastidiosa is the causal agent of citrus variegated chlorosis (cvc) and has been associated with important losses in commercial orchards of all sweet orange [citrus sinensis (l.)] cultivars. the development of this disease depends on the environmental conditions, including the endophytic microbial community associated with the host plant. previous studies have shown that x. fastidiosa interacts with the endophytic community in xylem vessels as well as in the insect vector, ... | 2016 | 27727362 |
| antibacterial activity of six medicinal cameroonian plants against gram-positive and gram-negative multidrug resistant phenotypes. | infectious diseases due to multidrug-resistant bacteria are one of the causes of treatment failures contributing to an increase in mortality and/or morbidity. in this study, we evaluated the antibacterial potential of different parts of six medicinal plants namely alstonia boonei, ageratum conyzoides, croton macrostachys, cassia obtusifolia, catharanthus roseus and paullinia pinnata against a panel of 36 multi-drug resistant (mdr) gram-negative and gram-positive bacteria. | 2016 | 27724917 |
| cdia effectors from uropathogenic escherichia coli use heterotrimeric osmoporins as receptors to recognize target bacteria. | many gram-negative bacterial pathogens express contact-dependent growth inhibition (cdi) systems that promote cell-cell interaction. cdi+ bacteria express surface cdia effector proteins, which transfer their c-terminal toxin domains into susceptible target cells upon binding to specific receptors. cdi+ cells also produce immunity proteins that neutralize the toxin domains delivered from neighboring siblings. here, we show that cdiaec536 from uropathogenic escherichia coli 536 (ec536) uses ompc a ... | 2016 | 27723824 |
| detection of cronobacter sakazakii in powdered infant formula using an immunoliposome-based immunomagnetic concentration and separation assay. | this study aimed to optimize the applicability of an immunoliposome-based immunomagnetic concentration and separation assay to facilitate rapid detection of cronobacter sakazakii in powdered infant formula (pif). to determine the detection limit, specificity, and pre-enrichment incubation time (0, 4, 6, and 8 h), assay tests were performed with different cell numbers of c. sakazakii (2 × 10(0) and 2 × 10(1) cfu/ml) inoculated in 10 g of pif. the assay was able to detect as few as 2 cells of c. s ... | 2016 | 27721500 |
| characterization of escherichia coli and other enterobacteriaceae in producer-distributor bulk milk. | the current study was undertaken to characterize escherichia coli and other enterobacteriaceae in raw and pasteurized producer-distributor bulk milk (pdbm). a total of 258 samples were collected from purchase points in 8 provinces in south africa. the samples were tested for antibiotic residues, phosphatase, total aerobic bacteria, coliforms, and e. coli counts. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used for identification of isolates. escherichia coli ... | 2016 | 27720154 |
| impact of hiv infection on the presentation, outcome and host response in patients admitted to the intensive care unit with sepsis; a case control study. | sepsis is a prominent reason for intensive care unit (icu) admission in patients with hiv. we aimed to investigate the impact of hiv infection on presentation, outcome and host response in sepsis. | 2016 | 27719675 |
| carbapenem-resistant klebsiella pneumoniae influences the outcome of early infections in liver transplant recipients. | infections remain a leading cause of morbidity and mortality among liver transplant (lt) recipients. the aim of our study was to define the factors associated with outcome of early bacterial and fungal infections in a cohort of patients who underwent lt at the university hospital of ancona over a nine year period. | 2016 | 27716164 |
| antimicrobial susceptibility testing in predicting the presence of carbapenemase genes in enterobacteriaceae in south africa. | carbapenem-resistant enterobacteriaceae (cre) is a concern in south africa and worldwide. it is therefore important that these organisms be accurately identified for infection prevention control purposes. | 2016 | 27716102 |
| biodegradation of malathion, α- and β-endosulfan by bacterial strains isolated from agricultural soil in veracruz, mexico. | the objective of this study was to evaluate the capacity of two bacterial strains isolated, cultivated, and purified from agricultural soils of veracruz, mexico, for biodegradation and mineralisation of malathion (diethyl 2-(dimethoxyphosphorothioyl) succinate) and α- and β-endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6-9-methano-2,4,3-benzodioxathiepine-3-oxide). the isolated bacterial strains were identified using biochemical and morphological characterization and the analysis ... | 2016 | 27715499 |
| resistance mechanisms. | by definition, the terms sepsis and septic shock refer to a potentially fatal infectious state in which the early administration of an effective antibiotic is the most significant determinant of the outcome. because of the global spread of resistant bacteria, the efficacy of antibiotics has been severely compromised. s. pneumonia, escherichia coli (e. coli), klebsiella, acinetobacter, and pseudomonas are the predominant pathogens of sepsis and septic shock. it is common for e. coli, klebsiella, ... | 2016 | 27713884 |
| effects of selected diazotrophs on maize growth. | laboratory, greenhouse, and field experiments were conducted at the university of kwazulu-natal, pietermaritzburg, south africa in the 2010/2011 and 2011∖2012 seasons to study the effects of eight strains of diazotrophic bacteria on the growth and yield of maize. maize seeds were treated with bacillus megaterium (v16), pseudomonas sp. (stb5, a3, a6, and a61), burkholderia ambifaria (v9), enterobacter cloacae (l1) and pantoea ananatis (lb5), aiming to stimulate plant growth, and maintain or incre ... | 2016 | 27713756 |
| armadillidin h, a glycine-rich peptide from the terrestrial crustacean armadillidium vulgare, displays an unexpected wide antimicrobial spectrum with membranolytic activity. | antimicrobial peptides (amps) are key components of innate immunity and are widespread in nature, from bacteria to vertebrate animals. in crustaceans, there are currently 15 distinct amp families published so far in the literature, mainly isolated from members of the decapoda order. up to now, armadillidin is the sole non-decapod amp isolated from the haemocytes of armadillidium vulgare, a crustacean isopod. its first description demonstrated that armadillidin is a linear glycine-rich (47%) cati ... | 2016 | 27713732 |
| meropenem-induced neutropenia in a neonate. | postmarketing surveillance has associated meropenem with the development of hematologic abnormalities, including agranulocytosis, neutropenia, and leukopenia, but the exact incidence in children is unknown. the case describes a full-term, 26-day-old neonate admitted for a sepsis workup. she was found to have a blood culture positive for enterobacter cloacae and suspected meningitis and was initiated on meropenem 40 mg/kg/dose intravenously every 8 hours. on day 14 of antibiotic treatment, the pa ... | 2016 | 27713676 |
| a novel fe(iii) dependent bioflocculant from klebsiella oxytoca gs-4-08: culture conditions optimization and flocculation mechanism. | in this work, the effect of cultivation factors on the flocculation efficiency (fe) of bioflocculant p-gs408 from klebsiella oxytoca was optimized by the response surface methodology. the most significant factor, i.e. culture time, was determined by gray relational analysis. a total of 240 mg of purified p-gs408 was prepared from 1 liter of culture solution under the optimal conditions. gc-ms analysis results indicated that the polysaccharide of p-gs408 mainly contains rhamnose and galactose, an ... | 2016 | 27713559 |
| lessons learned from surveillance of antimicrobial susceptibilities of pseudomonas aeruginosa at a large academic medical center. | this research report assessed the differences in resistance rates and antimicrobial usage-versus-susceptibility relationships of pseudomonas aeruginosa found in various hospital patient care areas. a simplified case control study was also performed to identify patient-specific risk factors associated with cefepime-resistant p. aeruginosa isolates. last, we determined the consequence of combining mucoid and non-mucoid derived antimicrobial susceptibilities of p.aeruginosa into hospital antibiogra ... | 2010 | 27713288 |
| macrolide resistance in streptococcus pneumoniae. | streptococcus pneumoniae is a common commensal and an opportunistic pathogen. suspected pneumococcal upper respiratory infections and pneumonia are often treated with macrolide antibiotics. macrolides are bacteriostatic antibiotics and inhibit protein synthesis by binding to the 50s ribosomal subunit. the widespread use of macrolides is associated with increased macrolide resistance in s. pneumoniae, and the treatment of pneumococcal infections with macrolides may be associated with clinical fai ... | 2016 | 27709102 |
| are community-based hospitals safe from carbapenem-resistant enterobacteriaceae in korea? | 2016 | 27704734 | |
| extended-spectrum ß-lactamase-producing enterobacteriaceae as a common cause of urinary tract infections in sri lanka. | extended-spectrum ß-lactamase-producing enterobacteriaceae (esbl-pe) are increasingly reported as pathogens in urinary tract infections (utis). however, in sri lanka, the clinical and molecular epidemiology of esbl-pe implicated in utis has not been well described. | 2016 | 27704730 |
| expression, purification and immobilization of tannase from staphylococcus lugdunensis mtcc 3614. | enzymes find their applications in various industries, due to their error free conversion of substrate into product. tannase is an enzyme used by various industries for degradation of tannin. biochemical characterization of a specific enzyme from one organism to other is one of the ways to search for enzymes with better traits for industrial applications. here, tannase encoding gene from staphylococcus lugdunensis was cloned and suitability of the enzyme in various conditions was analysed to fin ... | 2016 | 27704471 |
| community-onset bloodstream and other infections, caused by carbapenemase-producing enterobacteriaceae: epidemiological, microbiological, and clinical features. | background. because most infections caused by carbapenemase-producing enterobacteriaceae (cpe) begin during hospitalization, there are limited data about community-onset (co) infections caused by cpe. our aim is to describe the frequency of co infections caused by cpe as well as the clinical features of co bloodstream infections (co-bsis). methods. this study includes retrospective case series of co infections caused by cpe in a tertiary hospital from january 2010 to july 2014. any clinical sa ... | 2016 | 27703997 |
| accuracy of conventional identification methods used for enterobacteriaceae isolates in three nigerian hospitals. | enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. multidrug-resistant enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. in developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria. in t ... | 2016 | 27703855 |
| therapeutic drug monitoring of meropenem in neonate with necrotizing enterocolitis: a challenge. | necrotizing enterocolitis (nec) continues to be a major cause of neonatal morbidity and mortality. we describe the added value of therapeutic drug monitoring by presenting the case of a preterm infant with severe nec treated with meropenem. dosing strategy will achieve adequate patient outcome when treating pathogens with elevated mic. as safe as meropenem is, there are not enough data for 40 mg/kg, every 8 h infused over 4 h; accordingly, strict monitoring of blood levels is mandatory. based on ... | 2016 | 27703820 |
| role of gut microbiota in the aetiology of obesity: proposed mechanisms and review of the literature. | the aetiology of obesity has been attributed to several factors (environmental, dietary, lifestyle, host, and genetic factors); however none of these fully explain the increase in the prevalence of obesity worldwide. gut microbiota located at the interface of host and environment in the gut are a new area of research being explored to explain the excess accumulation of energy in obese individuals and may be a potential target for therapeutic manipulation to reduce host energy storage. several me ... | 2016 | 27703805 |
| protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics. | a functional comparative genome analysis is essential to understand the mechanisms underlying bacterial evolution and adaptation. detection of functional orthologs using standard global sequence similarity methods faces several problems; the need for defining arbitrary acceptance thresholds for similarity and alignment length, lateral gene acquisition and the high computational cost for finding bi-directional best matches at a large scale. we investigated the use of protein domain architectures ... | 2016 | 27703668 |
| distribution of esbls, ampc β-lactamases and carbapenemases among enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the asia-pacific region during 2008-14: results from the study for monitoring antimicrobial resistance trends (smart). | to investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (esbls, ampc β-lactamases and carbapenemases) among enterobacteriaceae isolates that caused intra-abdominal infections (iais) in patients hospitalized in the asia-pacific region during 2008-14. | 2017 | 27703058 |
| aminoglycoside antibiotics for nih category ii chronic bacterial prostatitis: a single-cohort study with one-year follow-up. | although fluoroquinolones are first-line agents for the treatment of national institutes of health (nih) category ii chronic bacterial prostatitis (cbp), therapy with these agents is not always feasible due to the increasing worldwide resistance of causative uropathogens. new therapeutic options are urgently required, as drugs such as β-lactam antibiotics distribute poorly to prostatic sites of infection and trimethoprim therapy is often unfeasible due to high resistance rates. the present study ... | 2016 | 27698761 |
| bee venom (apis mellifera) an effective potential alternative to gentamicin for specific bacteria strains: bee venom an effective potential for bacteria. | mellitine, a major component of bee venom (bv, apis mellifera), is more active against gram positive than gram negative bacteria. moreover, bv has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. in addition, wasp venom has been reported to have antibacterial properties. the aim of this study was to evaluate the antibacterial activity of bv against selected gram positive and gram negative bacterial strains of me ... | 2016 | 27695631 |
| multidisciplinary approach to giant paratesticular liposarcoma. | primary paratesticular tumours are very rare and the spermatic cord (sct) is the most frequent site of origin, with 20% of malignancy. although liposarcoma is the most frequent histotype (46.6 %), less than 200 cases have been reported in the literature. we report the case of a 56-year-old man who presented a giant scrotal mass of 25 years of evolution and measuring 40 × 40 cm. it could be considered the greatest paratesticular liposarcoma described to date. computed tomogaphy (ct) revealed mass ... | 2016 | 27695588 |
| applicability of an in-house saponin-based extraction method in bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system for identification of bacterial and fungal species in positively flagged blood cultures. | we used an in-house saponin-based extraction method to evaluate the performance of the bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof/ms) system for the identification of bacteria and fungi in 405 positively flagged blood culture bottles. results obtained from maldi-tof/ms were compared with those obtained using conventional phenotypic identification methods. of the 405 positively flagged blood culture bottles, 365 showed monomicrobal grow ... | 2016 | 27695442 |
| role of nf-kβ factor rel2 during plasmodium falciparum and bacterial infection in anopheles dirus. | anopheles mosquitoes transmit malaria which is one of the world's most threatening diseases. anopheles dirus (sensu stricto) is among the main vectors of malaria in south east asia. the mosquito innate immune response is the first line of defence against malaria parasites during its development. the immune deficiency (imd) pathway, a conserved immune signaling pathway, influences anti-plasmodium falciparum activity in anopheles gambiae, an. stephensi and an. albimanus. the aim of the study was t ... | 2016 | 27688040 |
| national survey of colistin resistance among carbapenemase-producing enterobacteriaceae and outbreak caused by colistin-resistant oxa-48-producing klebsiella pneumoniae, france, 2014. | from january 2014 to december 2014, 972 consecutive non-replicate carbapenemase-producing enterobacteriaceae isolates from colonised or infected patients were collected at the associated french national reference centre as part of the french national survey on antimicrobial resistance. it included 577 klebsiella spp. (59%), 236 escherichia coli (24%), 108 enterobacter spp. (11%), 50 citrobacter spp. (5%), and a single salmonella spp. isolate (0.1%). of 561 k. pneumoniae isolates, 35 were found t ... | 2016 | 27685838 |
| the effect of tellurite on highly resistant freshwater aerobic anoxygenic phototrophs and their strategies for reduction. | six fresh water aerobic anoxygenic phototrophs (erythromicrobium ezovicum, strain e1; erythromicrobium hydrolyticum, e4(1); erythromicrobium ramosum, e5; erythromonas ursincola, kr99; sandaracinobacter sibiricus, rb 16-17; and roseococcus thiosulfatophilus, rb3) possessing high level resistance to teo₃(2-) and the ability to reduce it to elemental te were studied to understand their interaction with this highly toxic oxyanion. tested organic carbon sources, ph, and level of aeration all had an i ... | 2015 | 27682119 |
| carbapenem resistance in gram-negative bacteria: the not-so-little problem in the little red dot. | singapore is an international travel and medical hub and faces a genuine threat for import and dissemination of bacteria with broad-spectrum resistance. in this review, we described the current landscape and management of carbapenem resistance in gram-negative bacteria (gnb) in singapore. notably, the number of carbapenem-resistant enterobacteriaceae has exponentially increased in the past two years. resistance is largely mediated by a variety of mechanisms. polymyxin resistance has also emerged ... | 2016 | 27681907 |
| architecture of class 1, 2, and 3 integrons from gram negative bacteria recovered among fruits and vegetables. | the spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. to understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). although low levels of resistance have been detected, the bacter ... | 2016 | 27679611 |
| pharmacokinetics of gentamicin eluted from a regenerating bone graft substitute: in vitro and clinical release studies. | deep bone and joint infections (dbji) are directly intertwined with health, demographic change towards an elderly population, and wellbeing.the elderly human population is more prone to acquire infections, and the consequences such as pain, reduced quality of life, morbidity, absence from work and premature retirement due to disability place significant burdens on already strained healthcare systems and societal budgets.dbjis are less responsive to systemic antibiotics because of poor vascular p ... | 2016 | 27678329 |
| uv-b radiation stress causes alterations in whole cell protein profile and expression of certain genes in the rice phyllospheric bacterium enterobacter cloacae. | among the different types of uv radiation, uv-b radiation (280-315 nm) has gained much attention mainly due to its increasing incidence on the earth's surface leading to imbalances in natural ecosystems. this study deals with the effects of uv-b radiation on the proteome and gene expression in a rice phyllospheric bacterium, enterobacter cloacae. of the five bacteria isolated from rice leaves, e. cloacae showed the highest level of resistance to uv-b and total killing occurred after 8 h of conti ... | 2016 | 27672388 |
| occurence of arma and rmtb aminoglycoside resistance 16s rrna methylases in extended-spectrum β-lactamases producing escherichia coli in algerian hospitals. | the aim of this study, was to characterize the extended-spectrum-β-lactamases (esbls) producing clinical strains of escherichia coli isolated between january 2009 and june 2012 from algerian hospitals and to determine the prevalence of 16s rrna methylase among them. sixty-seven esbl-producers were detected among the 239 isolates included: 52 ctx-m-15-producers, 5 ctx-m-3-producers, 5 ctx-m-1-producers, 2 ctx-m-14-producers, 2 shv-12-producers and one tem-167-producer. among the esbl-producing st ... | 2016 | 27672380 |
| necrotizing fasciitis in a healthy pediatric patient caused by enterobacter cloacae and serratia marcescens: a discussion of diagnosis and management. | 2016 | 27670553 | |
| enrichment of the lung microbiome with gut bacteria in sepsis and the acute respiratory distress syndrome. | sepsis and the acute respiratory distress syndrome (ards) are major causes of mortality without targeted therapies. although many experimental and clinical observations have implicated gut microbiota in the pathogenesis of these diseases, culture-based studies have failed to demonstrate translocation of bacteria to the lungs in critically ill patients. here, we report culture-independent evidence that the lung microbiome is enriched with gut bacteria both in a murine model of sepsis and in human ... | 2016 | 27670109 |
| identification and molecular characterization of bacteriophage phiaxp-2 of achromobacter xylosoxidans. | a novel achromobacter xylosoxidans bacteriophage, phiaxp-2, was isolated from hospital sewage in china. the phage was morphologically and microbiologically characterized, and its one-step growth curve, host range, genomic sequence, and receptor were determined. its morphology showed that phiaxp-2 belongs to the family siphoviridae. microbiological characterization demonstrated that ph 7 is most suitable for phage phiaxp-2; its titer decreased when the temperature exceeded 50 °c; phiaxp-2 is sens ... | 2016 | 27669904 |
| the dissemination of multidrug-resistant enterobacter cloacae throughout the uk and ireland. | enterobacter cloacae is a clinically important gram-negative member of the enterobacteriaceae, which has increasingly been recognized as a major pathogen in nosocomial infections. despite this, knowledge about the population structure and the distribution of virulence factors and antibiotic-resistance determinants of this species is scarce. in this study, we analysed a systematic collection of multidrug-resistant e. cloacae isolated between 2001 and 2011 from bloodstream infections across hospit ... | 2016 | 27669519 |
| open surgical versus minimal invasive necrosectomy of the pancreas-a retrospective multicenter analysis of the german pancreatitis study group. | necrotising pancreatitis, and particularly infected necrosis, are still associated with high morbidity and mortality. since 2011, a step-up approach with lower morbidity rates compared to initial open necrosectomy has been established. however, mortality and complication rates of this complex treatment are hardly studied thereafter. | 2016 | 27668746 |
| bacterial etiology and antibiotic resistance profile of community-acquired urinary tract infections in a cameroonian city. | introduction. community-acquired urinary tract infections (cautis) are usually treated empirically. geographical variations in etiologic agents and their antibiotic sensitivity patterns are common. knowledge of antibiotic resistance trends is important for improving evidence-based recommendations for empirical treatment of utis. our aim was to determine the major bacterial etiologies of cautis and their antibiotic resistance patterns in a cosmopolitan area of cameroon for comparison with prescri ... | 2016 | 27667998 |
| functional metagenomics of spacecraft assembly cleanrooms: presence of virulence factors associated with human pathogens. | strict planetary protection practices are implemented during spacecraft assembly to prevent inadvertent transfer of earth microorganisms to other planetary bodies. therefore, spacecraft are assembled in cleanrooms, which undergo strict cleaning and decontamination procedures to reduce total microbial bioburden. we wanted to evaluate if these practices selectively favor survival and growth of hardy microorganisms, such as pathogens. three geographically distinct cleanrooms were sampled during the ... | 2016 | 27667984 |
| ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate. | new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ... | 2016 | 27667823 |
| the differential importance of mutations within ampd in cephalosporin resistance of enterobacter aerogenes and enterobacter cloacae. | mechanisms leading to carbapenem and cephalosporin resistance were sought in enterobacter aerogenes isolates that were highly resistant to carbapenems but had no known carbapenemase. results were compared with recent work examining carbapenem-resistant enterobacter cloacae. eighteen carbapenem-resistant e. aerogenes were screened for known β-lactamase and carbapenemase genes, and novel carbapenemases were sought in whole-genome sequencing (wgs) data of the three most resistant isolates. for all ... | 2016 | 27665520 |
| comprehensive laboratory evaluation of a highly specific lateral flow assay for the presumptive identification of bacillus anthracis spores in suspicious white powders and environmental samples. | we conducted a comprehensive, multiphase laboratory evaluation of the anthrax biothreat alert(®) test strip, a lateral flow immunoassay (lfa) for the rapid detection of bacillus anthracis spores. the study, conducted at 2 sites, evaluated this assay for the detection of spores from the ames and sterne strains of b. anthracis, as well as those from an additional 22 strains. phylogenetic near neighbors, environmental background organisms, white powders, and environmental samples were also tested. ... | 2016 | 27661796 |
| splenic abscess owing to cancer at the splenic flexure: a case report and comprehensive review. | the cancer of the splenic flexure of the colon is a rare medical entity with severe morbidity because of its insidious onset. | 2016 | 27661050 |
| carbapenem-resistant enterobacteriaceae: prevalence and risk factors in a single community-based hospital in korea. | carbapenemase-producing enterobacteriaceae (cpe) are gram-negative bacteria with increasing prevalence of infection worldwide. in korea, 25 cases of cpe isolates were reported by the korea centers for disease control and prevention in 2011. most cpe cases were detected mainly at tertiary referral hospitals. we analyzed the prevalence and risk factors for carbapenem-resistant enterobacteriaceae (cre) in a mid-sized community-based hospital in korea. | 2016 | 27659436 |
| extraction of total nucleic acids from bacterial isolates using the biomérieux nuclisens easymag total nucleic acid extractor. | the biomerieux nuclisens easymag total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. forty eight isolates were extracted, yielding quantifiable amounts of dna for all isolates. the easymag is appropriate for dna extraction from bacterial isolates and will be incorporated in the clinical laboratory. | 2016 | 27658494 |
| correction: clinical impact of maldi-tof ms identification and rapid susceptibility testing on adequate antimicrobial treatment in sepsis with positive blood cultures. | [this corrects the article doi: 10.1371/journal.pone.0156299.]. | 2016 | 27658295 |
| a review of shv extended-spectrum β-lactamases: neglected yet ubiquitous. | β-lactamases are the primary cause of resistance to β-lactams among members of the family enterobacteriaceae. shv enzymes have emerged in enterobacteriaceae causing infections in health care in the last decades of the twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. likely originated from a chromosomal penicillinase of klebsiella pneumoniae, shv β-lactamases currently encompass a large number of allelic vari ... | 2016 | 27656166 |
| munchausen syndrome disguised as gossypiboma: an interesting case. | 2016 | 27651853 | |
| cathelicidin antimicrobial peptides with reduced activation of toll-like receptor signaling have potent bactericidal activity against colistin-resistant bacteria. | the world is at the precipice of a postantibiotic era in which medical procedures and minor injuries can result in bacterial infections that are no longer effectively treated by antibiotics. cathelicidins are peptides produced by animals to combat bacterial infections and to regulate innate immune responses. however, cathelicidins are potent activators of the inflammatory response. cathelicidins with reduced proinflammatory activity and potent bactericidal activity in the low micromolar range ag ... | 2016 | 27651360 |
| [analysis of the pathogenic characteristics of 162 severely burned patients with bloodstream infection]. | to analyze the distribution and drug resistance of pathogen isolated from severely burned patients with bloodstream infection, so as to provide reference for the clinical treatment of these patients. | 2016 | 27647068 |
| zinc pyrithione improves the antibacterial activity of silver sulfadiazine ointment. | pseudomonas aeruginosa, acinetobacter baumannii, and staphylococcus aureus are commonly associated with biofilm-associated wound infections that are recalcitrant to conventional antibiotics. as an initial means to identify agents that may have a greater propensity to improve clearance of wound-associated bacterial pathogens, we screened a food and drug administration-approved drug library for members that display bactericidal activity toward 72-h-established p. aeruginosa biofilms using an adeny ... | 2016 | 27642637 |
| prevalence and identification of burkholderia pseudomallei and near-neighbor species in the malabar coastal region of india. | accurate identification of pathogens with biowarfare importance requires detection tools that specifically differentiate them from near-neighbor species. burkholderia pseudomallei, the causative agent of a fatal disease melioidosis, is one such biothreat agent whose differentiation from its near-neighbor species is always a challenge. this is because of its phenotypic similarity with other burkholderia species which have a wide spread geographical distribution with shared environmental niches. m ... | 2016 | 27632353 |
| development of 11-plex mol-pcr assay for the rapid screening of samples for shiga toxin-producing escherichia coli. | strains of shiga toxin-producing escherichia coli (stec) are a serious threat to the health, with approximately half of the stec related food-borne illnesses attributable to contaminated beef. we developed an assay that was able to screen samples for several important stec associated serogroups (o26, o45, o103, o104, o111, o121, o145, o157) and three major virulence factors (eae, stx 1 , stx 2) in a rapid and multiplexed format using the multiplex oligonucleotide ligation-pcr (mol-pcr) assay che ... | 2016 | 27630828 |
| whole-genome multilocus sequence typing of extended-spectrum-beta-lactamase-producing enterobacteriaceae. | molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. whole-genome multilocus sequence typing (wgmlst) has emerged as a whole-genome sequencing (wgs)-based gene-by-gene typing method that may overcome these limitations and has been applied successfully for severa ... | 2016 | 27629900 |
| dental plaque bacteria with reduced susceptibility to chlorhexidine are multidrug resistant. | chlorhexidine (chx) is used in oral care products to help control dental plaque. in this study dental plaque bacteria were grown on media containing 2 μg/ml chlorhexidine gluconate to screen for bacteria with reduced chx susceptibility. the isolates were characterized by 16s rrna gene sequencing and antibiotic resistance profiles were determined using the disc diffusion method. | 2016 | 27629863 |
| zymomonas mobilis as a model system for production of biofuels and biochemicals. | zymomonas mobilis is a natural ethanologen with many desirable industrial biocatalyst characteristics. in this review, we will discuss work to develop z. mobilis as a model system for biofuel production from the perspectives of substrate utilization, development for industrial robustness, potential product spectrum, strain evaluation and fermentation strategies. this review also encompasses perspectives related to classical genetic tools and emerging technologies in this context. | 2016 | 27629544 |
| type 3 fimbriae encoded on plasmids are expressed from a unique promoter without affecting host motility, facilitating an exceptional phenotype that enhances conjugal plasmid transfer. | horizontal gene transfer (hgt), the transmission of genetic material to a recipient that is not the progeny of the donor, is fundamental in bacterial evolution. hgt is often mediated by mobile genetic elements such as conjugative plasmids, which may be in conflict with the chromosomal elements of the genome because they are independent replicons that may petition their own evolutionary strategy. here we study differences between type 3 fimbriae encoded on wild type plasmids and in chromosomes. u ... | 2016 | 27627107 |
| matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (maldi-tof ms) based microbial identifications: challenges and scopes for microbial ecologists. | matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (maldi-tof ms) based biotyping is an emerging technique for high-throughput and rapid microbial identification. due to its relatively higher accuracy, comprehensive database of clinically important microorganisms and low-cost compared to other microbial identification methods, maldi-tof ms has started replacing existing practices prevalent in clinical diagnosis. however, applicability of maldi-tof ms in the area of micr ... | 2016 | 27625644 |
| an irish outbreak of new delhi metallo-β-lactamase (ndm)-1 carbapenemase-producing enterobacteriaceae: increasing but unrecognized prevalence. | carbapenemase-producing enterobacteriaceae (cpe) may cause healthcare-associated infections with high mortality rates. new delhi metallo-β-lactamase-1 (ndm-1) is among the most recently discovered carbapenemases. | 2016 | 27624807 |
| maldi-tof ms versus vitek(®)2: comparison of systems for the identification of microorganisms responsible for bacteremia. | we evaluated the reliability and accuracy of the combined use of maldi-tof ms and classical id vitek2 to identify monomicrobial infection in blood culture bottles. in total, 70 consecutive positive blood cultures were included in this study. positive blood culture bottles were subjected to gram staining and subcultured on solid media. isolates grown from such culture media were used for classical id using vitek2 system. in parallel, an aliquot was subjected to a lysing-centrifugation method and ... | 2016 | 27620383 |
| structural differences in gut bacteria communities in developmental stages of natural populations of lutzomyia evansi from colombia's caribbean coast. | lutzomyia evansi, a phlebotomine insect endemic to colombia's caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in lutzomyia evansi. in this study, conventional microbiological methods and molecular tools were used to assess the compositio ... | 2016 | 27618991 |
| causality of small and large intestinal microbiota in weight regulation and insulin resistance. | the twin pandemics of obesity and type 2 diabetes (t2d) are a global challenge for health care systems. changes in the environment, behavior, diet, and lifestyle during the last decades are considered the major causes. a western diet, which is rich in saturated fat and simple sugars, may lead to changes in gut microbial composition and physiology, which have recently been linked to the development of metabolic diseases. | 2016 | 27617199 |
| use of maldi-tof ms and culturomics to identify mosquitoes and their midgut microbiota. | mosquitoes transmit a wide range of human parasitic and viral diseases. in recent years, new techniques such as maldi-tof ms have been developed to identify mosquitoes at the species level, which is key for entomological surveys. additionally, there is increasing interest in the mosquito microbiota and its role in vector capacity. | 2016 | 27613238 |
| in vitro model of production of antibodies; a new approach to reveal the presence of key bacteria in polymicrobial environments. | there is a rapid emergence of multiple resistant gram-negative bacteria due to overuse of antibiotics in the treatment of infections. biofilms consist of polymicrobial communities that survive the host's defense system. the key bacteria in biofilms are slow growing and support an attachment and rapid growth of other microorganisms. current antimicrobial strategies often fail due to poor diagnosis of key pathogens in biofilms. the study aims to develop anti-bacterial human antibodies in vitro fro ... | 2016 | 27612600 |
| opportunistic infections in hiv-infected patients differ strongly in frequencies and spectra between patients with low cd4+ cell counts examined postmortem and compensated patients examined antemortem irrespective of the haart era. | aids-related mortality has changed dramatically with the onset of highly active antiretroviral therapy (haart), which has even allowed compensated hiv-infected patients to withdraw from secondary therapy directed against opportunistic pathogens. however, in recently autopsied hiv-infected patients, we observed that associations with a broad spectrum of pathogens remain, although detailed analyses are lacking. therefore, we focused on the possible frequency and spectrum shifts in pathogens associ ... | 2016 | 27611681 |
| sequence-based screening for rare enzymes: new insights into the world of amdases reveal a conserved motif and 58 novel enzymes clustering in eight distinct families. | arylmalonate decarboxylases (amdases, ec 4.1.1.76) are very rare and mostly underexplored enzymes. currently only four known and biochemically characterized representatives exist. however, their ability to decarboxylate α-disubstituted malonic acid derivatives to optically pure products without cofactors makes them attractive and promising candidates for the use as biocatalysts in industrial processes. until now, amdases could not be separated from other members of the aspartate/glutamate racema ... | 2016 | 27610105 |
| bacteriophages in clinical samples can interfere with microbiological diagnostic tools. | bacteriophages are viruses that infect bacteria, and they are found everywhere their bacterial hosts are present, including the human body. to explore the presence of phages in clinical samples, we assessed 65 clinical samples (blood, ascitic fluid, urine, cerebrospinal fluid, and serum). infectious tailed phages were detected in >45% of ascitic fluid and urine samples. three examples of phage interference with bacterial isolation were observed. phages prevented the confluent bacterial growth re ... | 2016 | 27609086 |
| incn st7 epidemic plasmid carrying blaimp-4 in enterobacteriaceae isolates with epidemiological links to multiple geographical areas in china. | to characterize blaimp-4-carrying plasmids originating from inpatients in hong kong. | 2017 | 27609049 |
| process optimization on micro-aeration supply for high production yield of 2,3-butanediol from maltodextrin by metabolically-engineered klebsiella oxytoca. | an optimization process with a cheap and abundant substrate is considered one of the factors affecting the price of the production of economical 2,3-butanediol (2,3-bd). a combination of the conventional method and response surface methodology (rsm) was applied in this study. the optimized levels of ph, aeration rate, agitation speed, and substrate concentration (maltodextrin) were investigated to determine the cost-effectiveness of fermentative 2,3-bd production by metabolically-engineered kleb ... | 2016 | 27603922 |
| results from the canadian nosocomial infection surveillance program on carbapenemase-producing enterobacteriaceae, 2010 to 2014. | carbapenemase-producing enterobacteriaceae (cpe) are increasing globally; here we report on the investigation of cpe in canada over a 5-year period. participating acute care facilities across canada submitted carbapenem-nonsusceptible enterobacteriaceae from 1 january 2010 to 31 december 2014 to the national microbiology laboratory. all cpe were characterized by antimicrobial susceptibilities, pulsed-field gel electrophoresis, multilocus sequence typing, and plasmid restriction fragment length p ... | 2016 | 27600052 |
| performance of an in-house real-time polymerase chain reaction for identification of mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting. | brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. in the present study, an in-house real-time polymerase chain reaction (pcr) assay targeting the mpt64 gene for identification of mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. from may 2011 to july 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or pra-hsp ... | 2016 | 27598243 |
| assessment of genetically engineered trabulsiella odontotermitis as a 'trojan horse' for paratransgenesis in termites. | the formosan subterranean termite, coptotermes formosanus is an invasive urban pest in the southeastern usa. paratransgenesis using a microbe expressed lytic peptide that targets the termite gut protozoa is currently being developed for the control of formosan subterranean termites. in this study, we evaluated trabulsiella odontotermitis, a termite-specific bacterium, for its potential to serve as a 'trojan horse' for expression of gene products in termite colonies. | 2016 | 27595984 |
| metabolic engineering of zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars. | to develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production from biomass, we seek to redirect the carbon flow of the model ethanologen zymomonas mobilis to produce desirable hydrocarbon intermediate 2,3-butanediol (2,3-bdo). 2,3-bdo is a bulk chemical building block, and can be upgraded in high yields to gasoline, diesel, and jet fuel. | 2016 | 27594916 |
| extended-spectrum β-lactamase producing enterobacteriaceae in bulk tank milk from german dairy farms. | although the dairy farm environment is a known source of extended-spectrum β-lactamase (esbl)-producing bacteria, surveillance data on esbl in the milk production chain are still scarce. this study aimed at estimating the dimensions of the problem for public health and animal welfare by surveying esbl-producing enterobacteriaceae in raw bulk tank milk in germany. samples from 866 dairy farms, comprising about 1% of the total number of dairy farms in germany, were first screened for presence of c ... | 2016 | 27592073 |
| enhancement of calibrachoa growth, secondary metabolites and bioactivity using seaweed extracts. | calibrachoa x hybrida (solanaceae) cultivars are widely used in north and south america as ornamental plants. their potential as a source of antimicrobial compounds might be enhanced by seaweed extract (swe) applications. | 2016 | 27589868 |