Publications

infection due to chromobacteria.		1951	14880467
infection due to chromobacteria; report of 11 cases.		1951	14867953
toward pku enzyme replacement therapy: pegylation with activity retention for three forms of recombinant phenylalanine hydroxylase.	phenylketonuria (pku) is a disease in which phenylalanine and phenylalanine-derived metabolites build up to neurotoxic levels due to mutations in the phenylalanine hydroxylase gene (pah). enzyme replacement therapy is a viable option to supply active pah. however, the inherent protease sensitivity and potential immunogenicity of pah have precluded adoption of this approach. in this report, we have used polyethylene glycol derivatization (pegylation) to produce protected forms of pah for potentia ...	2004	14741785
characterisation of steryl esterase activities in commercial lipase preparations.	triglycerides, steryl esters, resin acids, free fatty acids and sterols are lipophilic extractives of wood (commonly referred to as pitch or wood resin) and have a negative impact on paper machine runnability and quality of paper. thus, enzymes capable of modifying these compounds would be potential tools for reducing pitch problems during paper manufacture. in this work, 19 commercial lipase preparations were tested for their ability to degrade steryl esters, which may play a significant role i ...	2004	14741769
microbial genomics: tropical treasure?	a brazilian consortium has unveiled the genomic dna sequence of the purple-pigmented bacterium chromobacterium violaceum, a dominant component of the tropical soil microbiota. the sequence provides insight into the abundant potential of this organism for biotechnological and pharmaceutical applications.	2004	14738751
lipase-catalyzed kinetic resolution on solid-phase via a "capture and release" strategy.	the lipase-catalyzed kinetic resolution of (r/s)-3-phenylbutyric acid 2 using solid-supported cyclohexane-1,3-dione (chd) 6 is described. in each case the predominant enantiomer observed, after cleavage from the resin, was (r)-(-)-3-phenylbutyric acid (r)-2 (ee > 99%) rather than the expected (s)-enantiomer of 2. this observation is in contrast to the fact that chromobacterium viscosum lipase shows high enantiospecificity for the (s)-enantiomer in the corresponding solution-phase hydrolysis reac ...	2003	14611219
cytotoxicity and potential antiviral evaluation of violacein produced by chromobacterium violaceum.	natural products are an inexhaustible source of compounds with promising pharmacological activities including antiviral action. violacein, the major pigment produced by chromobacterium violaceum, has been shown to have antibiotic, antitumoral and anti-trypanosoma cruzi activities. the goal of the present work was to evaluate the cytotoxicity of violacein and also its potential antiviral properties. the cytotoxicity of violacein was investigated by three methods: cell morphology evaluation by inv ...	2003	14595466
production of acylated homoserine lactones by different serotypes of vibrio anguillarum both in culture and during infection of rainbow trout.	onehundred and forty-eight out of onehundred and fifty strains of vibrio anguillarum isolated from vibriosis in danish marine aquaculture produced bacterial communication signals, acylated homoserine lactones, eliciting a response in the agrobacterium tumefaciens (pzlr4) monitoring system. one strain, a serotype o4, induced a strong response in the chromobacterium violaceum (cv026) monitoring system. profiles of ahls determined by tlc separation revealed the presence of at least four ahls and a ...	2003	14529176
improvement in enzyme activity and stability by addition of low molecular weight polyethylene glycol to sodium bis(2-ethyl-l-hexyl)sulfosuccinate/isooctane reverse micellar system.	the activity and stability of chromobacterium viscosum lipase (glycerolester hydrolase, ec 3.1.1.3)-catalyzed olive oil hydrolysis in sodium bis (2-ethyl-l-hexyl)sulfosuccinate (aot)/isooctane reverse micelles is increased appreciably when low molecular weight polyethylene glycol (peg 400) is added to the reverse micelles. to understand the effect of peg 400 on the phase behavior of the reverse micellar system, the phase diagram of aot/ peg 400/water/isooctane system was studied. the influences ...	2003	14515025
the complete genome sequence of chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability.	chromobacterium violaceum is one of millions of species of free-living microorganisms that populate the soil and water in the extant areas of tropical biodiversity around the world. its complete genome sequence reveals (i) extensive alternative pathways for energy generation, (ii) approximately 500 orfs for transport-related proteins, (iii) complex and extensive systems for stress adaptation and motility, and (iv) widespread utilization of quorum sensing for control of inducible systems, all of ...	2003	14500782
[isolation of chromobacterium prodigiosum in a case of chronic atypical pneumonia].		1961	14473317
association of pigment with the cell envelope of serratia marcescens (chromobacterium prodigiosum).		1960	14435346
formation of 5-hydroxytryptophan from tryptophan by chromobacterium violaceum.		1955	14394084
[pathogenicity of serratia marcescens or chromobacterium prodigiosum].		1964	14292685
immunological properties of bacterial cell wall mucopeptides.		1965	14268620
[immunochemical analysis of protein fractions of chromogenic (yellow) bacteria isolated from the blood of paratyphoid patients].		1964	14263623
cyanide formation by chromobacterium violaceum.	michaels, ruth (columbia university, new york, n.y.), and w. a. corpe. cyanide formation by chromobacterium violaceum. j. bacteriol. 89:106-112. 1965.-the formation of cyanide by a chromobacterium violaceum strain was studied with growing cultures and with nonproliferating cells grown in complex and chemically defined media. most of the cyanide was produced during the log-phase growth of the organism, and accumulated in the culture supernatant fluid. a synergistic effect of glycine and methionin ...	1965	14255648
[results of a 7 years survey on enterobacteriaceae from butcher's meat in dalat and surroundings (central vietnam)].		1964	14250522
factors influencing growth and polysaccharide formation by strains of chromobacterium violaceum.	corpe, william a. (columbia university, new york, n.y.). factors influencing growth and polysaccharide formation by strains of chromobacterium violaceum. j. bacteriol. 88:1433-1441. 1964.-gelatinous chromobacterium violaceum strains elaborate a fibrous exopolysaccharide that forms a matrix in which growing cells are embedded. stable, nongelatinous variants arising from gelatinous cultures were of two types: (i) those that had lost the ability to produce polysaccharide, and (ii) those that had lo ...	1964	14234803
effect of mutation on dna-composition of some bacteria.		1964	14218440
[a comparative study of the nuclease activity of various species of saprophytic bacteria].		1964	14208279
the effects of selected strains of pigmented microorganisms on small free-living amoebae.		1964	14206897
6-hydroxytryptophan formation by chromobacterium violaceum.		1964	14206055
a humoral antibody associated with endotoxin resistance.		1964	14200101
the synthesis of n-acetyl-alpha-and n-acetyl-beta-d-glucosamine i-phosphates (2-acetamido-2-deoxy-alpha- and beta-d-glucose i-phosphates).		1964	14192914
[comparative evaluation of the effectiveness of bacteria-trapping devices in the determination of bacterial concentrations of aerosols].		1963	14187229
isolation of d-fucosamine from bacillus cereus.		1964	14167834
infection and the water-closet.		1964	14133601
urease activity of intact and disrupted bacteria.		1964	14120692
some applications of deoxyribonucleic acid base composition in bacterial taxonomy.		1963	14108436
modification of host responses to bacterial endotoxins. i. specificity of pyrogenic tolerance and the role of hypersensitivity in pyrogenicity, lethality, and skin reactivity.	evidence is presented suggesting that the apparent non-specificity of pyrogenic tolerance observed with gram-negative bacterial endotoxins is due to related antigenic determinants associated with the macromolecular toxins. this is based on results obtained in rabbits from pyrogenic cross-tolerance tests with selected endotoxins. in these tests, purified endotoxins from escherichia coli (coo8) and chromobacterium violaceum (cv) gave results one might expect with non-reciprocal cross-reacting anti ...	1963	14078002
decomposition and binding action of a polysaccharide from chromobacterium violacium in soil.	martin, j. p. (university of california, riverside) and s. j. richards. decomposition and binding action of a polysaccharide from chromobacterium violaceum in soil. j. bacteriol. 85:1288-1294. 1963.-the decomposition rate and binding effect in soil of a polysaccharide from chromobacterium violaceum was compared with that of a variety of bacterial and plant polysaccharides and more complex organic residues. most of the polysaccharides tested decomposed more readily than mature plant residues and ...	1963	14047219
extracellular accumulation of pyrroles in bacterial cultures.	aerobacter aerogenes, paracolobactrum aerogenoides, spirillum serpens, and gelatinous strains of chromobacterium violaceum produced an extracellular, ether-soluble, ehrlich-positive substance when grown in media prepared with gelatin hydrolysate. the substance has been tentatively identified as pyrrole-2-carboxylic acid. both hydroxy-l-proline and allo-d-hydroxyproline have been shown to be precursors of the material. gelatinous strains of chromobacterium violaceum, but not the other positive cu ...	1963	14023126
[apropos of the use of sodium gluatmate in lyophilization of bacteria].		1963	14020341
peroxides and mutations.		1963	14002158
characterization of a 4-amino-4:6-dideoxy aldohexose from chromobacterium violaceum.		1962	14000219
studies on the cell wall of chromobacterium violaceum: the separation of lipopolysaccharide and mucopeptide by phenol extraction of whole cells.		1963	14000218
isolation of a new amino sugar from chromobacterium violaceum.	smith, e. j. (duke university, durham, n.c.), j. m. leatherwood, and r. w. wheat. isolation of a new amino sugar from chromobacterium violaceum. j. bacteriol. 84:1007-1010. 1962.-an investigation of the amino sugars and reducing compounds of a lipopolysaccharide of chromobacterium violaceum revealed the presence of a previously unreported cationic reducing compound. evidence indicates that the compound is a 6-deoxyhexosamine with an amino group at other than the c-2 position.	1962	13989399
intracellular nucleotides isolated from chromobacterium violaceum.		1962	13989398
divergent pathways of indole metabolism in chromobacterium violaceum.		1962	13987555
isolation of guanosine 2',3'-cyclic phosphate from chromobacterium violaceum.		1962	13898776
[studies on the formation of volatile acids by microorganisms from the family of achromobacter. pseudomonas and chromobacterium by means of paper chromatography].		1959	13851129
physiological aspects of violacein biosynthesis in nonproliferating cells.		1959	13815863
incorporation of c14-labeled substrates into violacein.		1960	13815862
the extracellular polysaccharide of gelatinous strains of chromobacterium violaceum.		1960	13812063
infection due to chromobacteria. report of a case of pneumonia due to chromobacterium prodigiosum successfully treated with kanamycin.		1960	13799665
a pigment-producing spoilage bacterium responsible for violet discoloration of refrigerated market milk and cream.	a psychrophilic strain of bacteria identified as chromobacterium lividum was established as the causative agent of an outbreak of violet discoloration in refrigerated, pasteurized retail milk and cream. the organism was rod-shaped, gram-negative, and produced viscid colonies with abundant violet pigment on tryptone glucose yeast extract agar. growth was abundant at 4 c but none occurred at 37 c. growth in milk was characterized by a dark violet ring at the surface after a few days, and the deep ...	1961	13749823
accumulation of indole compounds in cultures of chromobacterium violaceum.		1961	13695636
the serology and pathogenicity of the genus chromobacterium.		1959	13654734
particulate-bound pigment of serratia marcescens (chromobacterium prodigiosum).		1959	13652969
isolation of chromobacterium prodigiosum from empyema.		1959	13641437
nutritional requirements of chromobacterium violaceum.		1959	13630861
production of hemolysis by chromobacterium violaceum.		1959	13626901
the isolation of d-fucosamine from the specific polysaccharide of chromobacterium violaceum (nctc 7917).		1958	13607435
[culture of bacteria by symbiotic procedure with a new form of metagenetic plate. ii].		1958	13595735
[culture of bacteria by symbiotic procedure with a new form of metagenetic plate. i].		1958	13595734
a water-soluble, diffusible pigment produced by a strain of serratia marcescens (chromobacterium prodigiosum).		1958	13590216
[chromobacteria in human pathology; human chromobacterium infections].		1958	13543121
[classification of pseudomonadaceae].		1957	13470460
the isolation of d-glycero-d-galactoheptose and other sugar components from the specific polysaccharide of chromobacterium violaceum (bn).		1957	13459897
pseudohaemoptysis due to chromobacterium prodigiosum.		1957	13417607
the change from polar to peritrichous flagellation in chromobacterium spp.		1956	13357717
cultural and biochemical characteristics of the genus chromobacterium.		1956	13357716
5-hydroxytryptophan formation and tryptophan metabolism in chromobacterium violaceum.		1956	13341050
nitrate reduction in the genus chromobacterium.		1956	13340700
[not available].		1955	13329728
morphological and physiological characteristics of the genus chromobacterium.		1956	13319251
[isolation of chromobacterium violaceum from hepatic lesions seen in a monkey cercopithecus cephus; study and pathogenecity].		1954	13218378
[biological research on gelatinolytic, fibrinolytic and antidotal enzymes present in culture filtrates of certain microorganisms and in particular in penicillium notatum, actinomyces griseus, bacillus subtilis, pseudomonas aeruginosa, proteus, chromobacterium prodigiosum and staphylococcus pyogenes].		1954	13195286
[the effect of chromobacterium prodigiosum on the growth of bacterium bifidum].		1954	13188176
outbreaks of disease in animals associated with chromobacterium violaceum. i. the disease in swine.		1954	13162910
bacterial endocarditis due to chromobacterium prodigiosum; report of a case.		1954	13149883
variation in pigmentation and morphology of colonies of gelatinous strains of chromobacterium species from soil.		1953	13096508
fatal infection by chromobacterium violaceum.		1953	13085740
studies on certain species of bacteria assigned to the genus chromobacterium.		1953	13022628
chromobacterial infections.		1952	12981933
novel bacteria degrading n-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria.	bacteria degrading the quorum-sensing (qs) signal molecule n-hexanoylhomoserine lactone were isolated from a tobacco rhizosphere. twenty-five isolates degrading this homoserine lactone fell into six groups according to their genomic rep-pcr and rrs pcr-rflp profiles. representative strains from each group were identified as members of the genera pseudomonas, comamonas, variovorax and rhodococcus: all these isolates degraded n-acylhomoserine lactones other than the hexanoic acid derivative, albei ...	2003	12904538
chromobacterium violaceum bacteremia: a case report.	chromobacterium violaceum is confined in tropical and subtropical regions, which can cause life-threatening disease. it is the only chromobacterium species that is pathogenic to humans. because of its rarity, clinicians often do not appreciate its importance when it is isolated. we report a fulminate fatal case of c. violaceum bacteremia in a 20-year-old male taiwanese. the clinical manifestations were fever and abdominal pain, followed by shock and pulmonary septic embolism. emergent laparotomy ...	2003	12886967
ym-254890, a novel platelet aggregation inhibitor produced by chromobacterium sp. qs3666.	a novel platelet aggregation inhibitor, ym-254890, was isolated from the culture broth of strain qs3666. this strain was isolated from a soil sample collected at okutama, tokyo, japan, and was identified as chromobacterium sp. by morphological and physiological criteria. ym-254890 was purified from the culture supernatant by solvent extraction, ods and silica gel flash chromatography, followed by preparative hplc. ym-254890 inhibited adp-induced platelet aggregation in human platelet-rich plasma ...	2003	12817809
[the inhibitory effect of extracts from galla chinensis on marine fouling bacteria].	to observe the activity of extracts from galla chinensis with different solvents against marine fouling bacteria in vitro.	2003	12795221
fatal septicaemia caused by chromobacterium violaceum.	we report a case of chromobacterium violaceum septicaemia in a 10-year old boy admitted to hospital in september 2001, two weeks after he suffered a crush injury of the left foot. in spite of surgical debridement and antibiotics his condition worsened and he developed multiple liver and lung abscesses. he died one week after admission and a blood culture grew chromobacterium violaceum.	2003	12795018
picosecond dynamics of a peptide from the acetylcholine receptor interacting with a neurotoxin probed by tailored tryptophan fluorescence.	a tryptophan analog, dehydro-n-acetyl-l-tryptophanamide (delta-nata), which is produced enzymatically via l-tryptophan 2',3'-oxidase from chromobacterium violaceum, is newly used for time-resolved fluorescence. the absorption and emission maxima of delta-nata at 332 and 417 nm, respectively, in 20% dimethylformamide-water are significantly shifted to the red with respect to those of tryptophan in water, permitting us to measure its fluorescence in the presence of tryptophan residues. we demonstr ...	2003	12785053
pcr-assisted contig extension: stepwise strategy for bacterial genome closure.	finishing is rate limiting for genome projects, and improvements in the efficiency of complete genome-sequence compilation will require improved protocols for gap closure. here we report a novel approach for extending shotgun contigs and closing gaps that we termed pcr-assisted contig extension (pace). pace depends on the capture of rare mismatched interactions that occur between arbitrary primers and template dna of unknown sequence, even under highly stringent conditions, by means of elevated ...	2003	12661167
violacein and its beta-cyclodextrin complexes induce apoptosis and differentiation in hl60 cells.	violacein, a pigment isolated from chromobacterium violaceum, has been reported to have multiple biological activities including in vitro antitumor effects. certain anticancer agents are known to induce apoptosis in human tumor cell lines. in this work, our aim was to investigate the effectiveness of violacein/beta-cyclodextrin (beta-cd)-containing systems to produce lethal effects in the human promyelocytic leukemia cell line hl60. using the mtt tetrazolium reduction test, ic(50) for the inclus ...	2003	12628314
phagocyte nadph oxidase, but not inducible nitric oxide synthase, is essential for early control of burkholderia cepacia and chromobacterium violaceum infection in mice.	reactive oxygen and nitrogen intermediates have critical, partially overlapping roles in host defense against a variety of pathogens. using mice deficient in generating phagocyte superoxide (p47(phox)(-/-)) and mice deficient in generating inducible nitric oxide synthase (inos(-/-)), we examined the roles of these reactive species in host defense against burkholderia cepacia and chromobacterium violaceum, organisms known to have unusual virulence in chronic granulomatous disease. intraperitoneal ...	2003	12496167
order of substrate binding in bacterial phenylalanine hydroxylase and its mechanistic implication for pterin-dependent oxygenases.	phenylalanine hydroxylase (pah) is a pterin-dependent non-heme metalloenzyme that catalyzes the oxidation of phenylalanine to tyrosine, which is the rate-limiting step in the catabolism of phe. chromobacterium violaceum phenylalanine hydroxylase (cpah) has been prepared and its steady-state mechanism has been investigated. the enzyme requires iron for maximal activity. initial rate measurements, done in the presence of the 6,7-dimethyl-5,6,7,8-tetrahydropterin (dmph(4)) cofactor, yielded an aver ...	2003	12459906
degradation of aliphatic polyester films by commercially available lipases with special reference to rapid and complete degradation of poly(l-lactide) film by lipase pl derived from alcaligenes sp.	commercial lipases were examined for their degradation efficiency of aliphatic polyester films. in 100 days immersion of polyester films in lipase solutions at 37 degrees c at ph 7.0, lipase asahi derived from chromobacterium viscosum degraded polybutylene succinate-co-adipate (pbsa), poly (e-caprolactone) (pcl) and polybutylene succinate (pbs), and lipase f derived from rhizopus niveus degraded pbsa and pcl during 4-17 days. lipase f-ap15 derived from rhizopus orizae could degrade pbsa in 22 da ...	2002	12449316
a phase i trial of depsipeptide (fr901228) in patients with advanced cancer.	depsipeptide (fr901228) is a bicyclic peptide isolated from chromobacterium violaceum that has demonstrated potent in vitro cytotoxic activity against human tumor cell lines and in vivo efficacy against human tumor xenografts. the primary mechanism of action is through inhibition of histone deacetylase. initial development was halted due to significant cardiac toxicity. subsequent studies performed at the national cancer institute demonstrated administration without cardiotoxicity was possible b ...	2002	12440223
crystal structure of a thermostable lipase from bacillus stearothermophilus p1.	we describe the first lipase structure from a thermophilic organism. it shares less than 20% amino acid sequence identity with other lipases for which there are crystal structures, and shows significant insertions compared with the typical alpha/beta hydrolase canonical fold. the structure contains a zinc-binding site which is unique among all lipases with known structures, and which may play a role in enhancing thermal stability. zinc binding is mediated by two histidine and two aspartic acid r ...	2002	12417199
chromobacterium violaceum infection in children: a case of fatal septicemia with nasopharyngeal abscess and literature review.	this previously healthy 5-year-old boy initially presented with fever and purulent conjunctivitis. the course evolved rapidly into preseptal and facial cellulitis, nasopharyngeal abscess and sepsis. chromobacterium violaceum was isolated from conjunctival exudate and blood cultures. he received intravenous cefazolin therapy for 2 days, followed by penicillin, oxacillin and netilmicin. however, no improvement was noted, and he died on the fifth days of illness.	2002	12237610
chromobacterium violaceum: a case of diarrhea in senegal.	a 5-year-old infant with diarrhea had heavy growth of chromobacterium violaceum cultured from stool. this organism is restricted geographically between latitudes 35 degrees n and 35 degrees s. it can cause sepsis and various focal infections but is not a well-known cause of diarrhea.	2002	12182388
characterization of a new efflux pump, mexghi-opmd, from pseudomonas aeruginosa that confers resistance to vanadium.	vanadium has an antibacterial activity against pseudomonas aeruginosa, especially under conditions of iron limitation. some degree of resistance to v is inducible by prior exposure to the metal. one mutant (vs1) with a higher sensitivity to v was obtained by transposon mutagenesis of p. aeruginosa pa 59.20, a clinical isolate. this mutant had an insertion in a non-coding region, upstream of a cluster of four genes. three of them show similarities to genes corresponding to known p. aeruginosa ant ...	2002	12177331
effect of medium composition, flow rate, and signaling compounds on the formation of soluble extracellular materials by biofilms of chromobacterium violaceum.	biofilms of the homoserine-lactone-defective strain chromobacterium violaceum cv026 were grown on silicone surfaces in a flow chamber. the effect of medium flow rate, different levels of n-butanoyl-homoserine lactone, and nutrients on biofilm activity was studied by quantifying the proteins and exopolysaccharides attached to the cells. to compare the effect of each of the three variables within a wide range, the experiments were designed as a full factorial search with three levels for each vari ...	2002	12111158
structural comparison of bacterial and human iron-dependent phenylalanine hydroxylases: similar fold, different stability and reaction rates.	structure determination of bacterial homologues of human disease-related proteins provides an efficient path to understanding the three-dimensional fold of proteins that are associated with human diseases. however, the precise locations of active-site residues are often quite different between bacterial and human versions of an enzyme, creating significant differences in the biological understanding of enzyme homologs. to study this hypothesis, phenylalanine hydroxylase from a bacterial source h ...	2002	12096915
[bromo- and iodo-containing alkaloids from marine microorganisms and sponges].	the taxonomic distribution, structure, and biological activity of halogenated alkaloids isolated from marine microorganisms and sponges are reviewed. the structures of nearly 140 natural bromo- and iodo-containing alkaloids are shown.	2002	12077845
building blocks for the solution phase synthesis of oligonucleotides: regioselective hydrolysis of 3',5'-di-o-levulinylnucleosides using an enzymatic approach.	a short and convenient synthesis of 3'- and 5'-o-levulinyl-2'-deoxynucleosides has been developed from the corresponding 3',5'-di-o-levulinyl derivatives by regioselective enzymatic hydrolysis, avoiding several tedious chemical protection/deprotection steps. thus, candida antartica lipase b (cal-b) was found to selectively hydrolyze the 5'-levulinate esters, furnishing 3'-o-levulinyl-2'-deoxynucleosides 3 in >80% isolated yields. on the other hand, immobilized pseudomonas cepacia lipase (psl-c) ...	2002	12076150
chromobacterium violaceum septicemia.	chromobacterium violaceum is an extremely rare human pathogen. we report a rare case of septicemia with multiple abscesses and otitis media in a newborn caused by chromobacterium violaceum.	2002	12019559
[synthesis of signaling n-acyl-homoserine-lactones participating in quorum sensing in rhizosphere and soil bacteria pseudomonas and xanthomonas].	signaling molecules assigned to n-acyl-homoserine-lactones (ahl) serve as autoinducers for the genes controlling the quorum sensing regulatory system. in many gram-negative bacteria, ahl are the key factors responsible for density-dependent regulation of exoenzyme and secondary metabolite production; they also participate in interaction between bacteria and higher organisms. the soil and rhisosphere bacteria pseudomonas and xanthomonas from different geographical zones of russia and the former u ...	2002	12018176
fluorescent inhibitors reveal solvent-dependent micropolarity in the lipid binding sites of lipases.	triacylglycerol analogue p-nitrophenyl phosphonates specifically react with the active-site serine of lipolytic enzymes to give covalent lipase-inhibitor complexes, mimicking the first transition state which is involved in lipase-mediated ester hydrolysis. here we report on a new type of phosphonate inhibitors containing a polarity-sensitive fluorophore to monitor micropolarity around the active site of the enzyme in different solvents. the respective compounds are hexyl and methyl dimethylamino ...	2002	12009403
synthesis of monoacyl a-ring precursors of 1alpha,25-dihydroxyvitamin d3 through selective enzymatic hydrolysis.	an efficient synthesis of monoacylated 1alpha,25-dihydroxyvitamin d3 a-ring precursors 15, 16, 18, and 19 has been described through an enzymatic hydrolysis process. candida antarctica a lipase (cal-a) hydrolyzes the c-5 acetate ester in trans stereoisomers 9 and 13, with complete and high selectivity, respectively. in the case of cis isomers 11 and 14, chromobacterium viscosum lipase (cvl) is the enzyme of choice, exhibiting opposite selectivity for these two enantiomers. this lipase selectivel ...	2002	11846672