Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| Hazara virus infection is lethal for adult type I interferon receptor-knockout mice and may act as a surrogate for infection with the human pathogenic Crimean-Congo Haemorrhagic Fever virus. | Hazara virus (HAZV) is closely related to Crimean-Congo Haemorrhagic Fever virus (CCHFV). HAZV has not been reported to cause human disease; work with infectious material can be carried out at containment level (CL)-2. By contrast, CCHFV causes a haemorrhagic fever in humans and requires CL-4 facilities. A disease model of HAZV infection in mice deficient in the type I interferon receptor is reported in this study. Dose response effects were seen, with higher doses resulting in a shorter time to ... | 2011 | 22090213 |
| parotitis associated with crimean congo hemorrhagic fever virus. | crimean congo hemorrhagic fever (cchf) is a potentially fatal tick-borne viral disease, the course of which may accompanied by various clinical findings. | 2011 | 22079303 |
| relationship between nf-κb1 and nf-κbia genetic polymorphisms and crimean-congo hemorrhagic fever. | background: crimean-congo hemorrhagic fever (cchf) is an acute viral hemorrhagic fever caused by the crimean-congo hemorrhagic fever virus (cchfv). nuclear factor (nf)-κb regulates the expression of hundreds of genes, including inflammatory and immunoregulatory, cell cycle regulating, and anti-apoptotic genes. nf-κbia (iκbα) encodes an inhibitory version of the nf-κb proteins. methods: this study is the first to investigate the association between nf-κb1 - 94w/d and nf-κbia 3→utr a→g polymorphis ... | 2011 | 22066734 |
| re-emergence of crimean-congo hemorrhagic fever virus in central africa. | crimean-congo hemorrhagic fever (cchf) is a severe tick-borne disease well recognized through europe and asia where diagnostic tests and medical surveillance are available. however, it is largely neglected in africa, especially in the tropical rainforest of central africa where only sporadic human cases have been reported and date back to more than 30 years. we describe here an isolated human case that occurred in the democratic republic of the congo in 2008 and performed phylogenetic analysis t ... | 2011 | 22022629 |
| mice orally immunized with a transgenic plant expressing the glycoprotein of crimean-congo hemorrhagic fever virus. | while crimean-congo hemorrhagic fever (cchf) has a high mortality rate in humans, the associated virus (cchfv) does not induce clinical symptoms in animals, but animals play an important role in disease transmission to humans. our aim in this study was to examine the immunogenicity of the cchfv glycoprotein when expressed in the root and leaf of transgenic plants via hairy roots and stable transformation of tobacco plants, respectively. after confirmatory analyses of transgenic plant lines and q ... | 2011 | 22012978 |
| Bacterial expression of Crimean-Congo hemorrhagic fever virus nucleoprotein and its evaluation as a diagnostic reagent in an indirect ELISA. | Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne viral zoonosis distributed widely in Africa, Asia, Russia and the Balkans. The emergence and re-emergence of CCHFV emphasize the importance of increasing both human and veterinary surveillance and developing diagnostic capacity. Recombinant CCHFV nucleocapsid protein (NP) has been expressed using insect cells and mammalian cells and used as a diagnostic tool but bacterial expression has not been described previously. The S gene of CCH ... | 2012 | 22001274 |
| impact of ebola mucin-like domain on antiglycoprotein antibody responses induced by ebola virus-like particles. | ebola virus (ebov) glycoprotein (gp), responsible for mediating host-cell attachment and membrane fusion, contains a heavily glycosylated mucin-like domain hypothesized to shield gp from neutralizing antibodies. to test whether the mucin-like domain inhibits the production and function of anti-gp antibodies, we vaccinated mice with ebola virus-like particles (vlps) that express vesicular stomatitis virus g, wild-type ebov gp (ebgp), ebov gp without its mucin-like domain (δmucgp), or ebov gp with ... | 2011 | 21987758 |
| production of cchf virus-like particle by a baculovirus-insect cell expression system. | crimean-congo haemorrhagic fever virus (cchfv) is a tick-born virus of the nairovirus genus within the bunyaviridae family, which is widespread and causes high fatality. the nucleocapsid of cchfv is comprised of n proteins that are encoded by the s segment. in this research, the n protein of cchfv was expressed in insect cells using a recombinant baculovirus. under an electron microscope, virus-like particles (vlps) with various size and morphology were observed in cytoplasmic vesicles in the in ... | 2011 | 21979573 |
| using next generation sequencing to identify yellow fever virus in uganda. | in october and november 2010, hospitals in northern uganda reported patients with suspected hemorrhagic fevers. initial tests for ebola viruses, marburg virus, rift valley fever virus, and crimean congo hemorrhagic fever virus were negative. unbiased pcr amplification of total rna extracted directly from patient sera and next generation sequencing resulted in detection of yellow fever virus and generation of 98% of the virus genome sequence. this finding demonstrated the utility of next generati ... | 2012 | 21962764 |
| tick cell lines for study of crimean-congo hemorrhagic fever virus and other arboviruses. | abstract continuous cell lines derived from many of the vectors of tick-borne arboviruses of medical and veterinary importance are now available. their role as tools in arbovirus research to date is reviewed and their potential application in studies of tick cell responses to virus infection is explored, by comparison with recent progress in understanding mosquito immunity to arbovirus infection. a preliminary study of propagation of the human pathogen crimean-congo hemorrhagic fever virus (cc ... | 2011 | 21955214 |
| prompt administration of crimean-congo hemorrhagic fever (cchf) virus hyperimmunoglobulin in patients diagnosed with cchf and viral load monitorization by reverse transcriptase-pcr. | crimean-congo hemorrhagic fever virus (cchfv), a member of the genus nairovirus of the family bunyaviridae, causes a severe disease in humans with high mortality rates. in turkey, the number of patients with cchf has increased since 2002. here, we aimed to treat cchf patients with cchfv hyperimmunoglobulin. we prepared a cchfv hyperimmunoglobulin product from 22 individuals who survived cchf infection. a total of 26 cchf patients were enrolled into this study. for cchfv hyperimmunoglobulin admin ... | 2011 | 21937830 |
| Species distribution and detection of Crimean Congo Hemorrhagic Fever Virus (CCHFV) in field-collected ticks in Ankara Province, Central Anatolia, Turkey. | Ticks may act as vectors for a number of infectious diseases including Crimean Congo Hemorrhagic Fever (CCHF). The causative agent is Crimean Congo Hemorrhagic Fever Virus (CCHFV), a member of Bunyaviridae, causing extensive ecchymosis, visceral bleeding and hepatic dysfunction with a high fatality rate in the affected individuals. CCHF was initially recognized in Turkey in 2002 and the current number of reported cases exceeds 4,400. This study was conducted to confirm the presence of tick speci ... | 2012 | 21910017 |
| [use of rt-pcr and enzyme immunoassays for the specific diagnosis of crimean hemorrhagic fever]. | the objective of the investigation was to evaluate the efficiency of the rt-pcr kit "amplysens chf" produced by interlabservice of the central research institute of epidemiology and that of the elisa kits made by the d. i. ivanovsky research institute of virology for the specific diagnosis of crimean hemorrhagic fever (chf). examination of sera from chf patients from the astrakhan region showed that positive rt-pcr results were observed in 95.2 and 37.5% on days 4-8 and 9-13 after disease onset, ... | 2011 | 21899068 |
| crimean-congo hemorrhagic fever serosurvey in at-risk professionals, madagascar, 2008 and 2009. | crimean-congo hemorrhagic fever (cchf) is a zoonotic arboviral infection with hemorrhagic manifestation and often a fatal ending. human become infected mainly through tick bite or by crushing infected tick, by contact with blood or tissues from viraemic livestock or patient. cchf virus (cchfv) has been isolated once in madagascar but data on the epidemiology of the disease in the country are very scarce. | 2011 | 21889395 |
| Recent advances in the molecular and cellular biology of bunyaviruses. | The family Bunyaviridae of segmented, negative-stranded RNA viruses includes over 350 members that infect a bewildering variety of animals and plants. Many of these bunyaviruses are the causative agents of serious disease in their respective hosts, and are classified as emerging viruses because of their increased incidence in new populations and geographical locations throughout the world. Emerging bunyaviruses, such as Crimean-Congo hemorrhagic fever virus, tomato spotted wilt virus and Rift Va ... | 2011 | 21865443 |
| Epidemiology and mutational analysis of global strains of Crimean-Congo haemorrhagic fever virus. | Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality. Cases are reported in several countries in Africa, Europe, the Middle East, and Asia. Phylogenetic analyses based on the virus S (nucleocapsid), M (glycoprotein), and L (polymerase) genome segments sequences indicate distinct geographic lineages exist but their specific genetic characteristics require elucidation. In this work we collected all full length S segment sequences and generated a phylogenetic tree based on ... | 2011 | 21847754 |
| cardiac findings in children with crimean-congo hemorrhagic fever. | background: crimean-congo hemorrhagic fever (cchf) involves the multi-organ systems. the involvement of the heart in adult patients has been described previously. we investigated the electrocardiographic and echocardiographic findings of pediatric patients with cchf.<br /> material/methods: patients younger than 16 years of age diagnosed with cchf were enrolled in the study. the diagnosis of cchf infection was based upon typical clinical and epidemiological findings and serological tests. all pa ... | 2011 | 21804465 |
| crimean-congo hemorrhagic fever virus in hyalommid ticks, northeastern kenya. | as part of ongoing arbovirus surveillance, we screened ticks obtained from livestock in northeastern kenya in 2008 to assess the risk for human exposure to tick-borne viruses. of 1,144 pools of 8,600 hyalomma spp. ticks screened for congo-crimean hemorrhagic fever virus by reverse transcription pcr, 23 pools were infected, demonstrating a potential for human exposure. | 2011 | 21801635 |
| crimean-congo haemorrhagic fever in india. | 2011 | 21784269 | |
| crimean-congo hemorrhagic fever: a molecular survey on hard ticks (ixodidae) in yazd province, iran. | to determine the rate of crimean-congo hemorrhagic fever virus (cchfv) infection in hard ticks (ixodidae) in yazd province of iran. | 2011 | 21771418 |
| crimean congo hemorrhagic fever: requires vigilance and not panic. | crimean congo hemorrhagic fever (cchf) has been in the news with reports of its outbreak in india from gujarat. cchf is caused by a virus which is a member of the nairovirus genus of the family bunyaviridae. all of these viruses are transmitted by either ixodid or argasid ticks. humans get this infection after a bite of an infected tick or from one infected human to another by contact with infectious blood or body fluids. workers in livestock and agriculture industry, slaughterhouses, and veteri ... | 2011 | 21751625 |
| crimean-congo hemorrhagic fever virus in ticks collected from humans, livestock, and picnic sites in the hyperendemic region of turkey. | abstract during june and july 2007, about 3125 adult ticks were collected from humans, animals, and vegetation in a hyperendemic region (sivas and tokat) of turkey. a total of 2193 ticks were pooled in 225 pools and screened for the crimean congo hemorrhagic fever virus (cchfv) presence by antigen-capture enzyme-linked immunosorbent assay. infection rates were calculated as the maximum likelihood estimation with 95% confidence intervals (ci). the dominant tick species was found to be hyalomma ma ... | 2011 | 21736490 |
| identification of a putative crimean-congo hemorrhagic fever virus entry factor. | entry of enveloped viruses into cells is initiated by binding of their envelope glycoproteins (envs) to cell surface-associated receptors. the crimean-congo hemorrhagic fever virus (cchfv) has two envs, gn and gc, with poorly understood role in binding to susceptible cells. we expressed codon optimized gn and gc, and identified independently folded soluble env fragments, one of which (gc residues 180-300) bound cchfv susceptible cells supposedly by interacting with a putative receptor. this rece ... | 2011 | 21723257 |
| evaluation of knowledge about protection against crimean-congo hemorrhagic fever. | this study was conducted in order to evaluate individuals' knowledge about protection against crimean congo hemorrhagic fever (cchf). this descriptive study was carried out among 478 persons, to whom a family health center located within boundaries of erzurum metropolitan municipality, provides health service. a questionnaire form developed by the researchers was used for collecting data. seventy-one point eight percent of individuals who participated in the study stated they had knowledge about ... | 2011 | 21706954 |
| development of a one step real time rt-pcr assay to detect and quantify dugbe virus. | a one-step real time quantitative rt-pcr (qrt-pcr) assay was developed to detect all published dugbe virus (dugv) genomes of the nairovirus genus. primers and probes were designed to detect specific sequences on the most conserved regions of the s segment. the limit of detection of the assay was 10 copies per reaction which is an improvement of 3 log(10)ffu/ml over the sensitivity of conventional rt-pcr. the specificity of the primers and probe was confirmed with the closely related nairoviruses ... | 2011 | 21703306 |
| a nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, sudan. | crimean-congo hemorrhagic fever (cchf), a tick-borne disease caused by crimean-congo hemorrhagic fever virus (cchfv), is a member of the genus nairovirus in the family bunyaviridae. recently, cchfv has been reported as an important emerging infectious viral pathogen in sudan. sporadic cases and multiple cchf outbreaks, associated with nosocomial chain of transmission, have been reported in the kordufan region of sudan. | 2011 | 21672268 |
| multiple crimean-congo hemorrhagic fever virus strains are associated with disease outbreaks in sudan, 2008-2009. | crimean-congo hemorrhagic fever (cchf) activity has recently been detected in the kordufan region of sudan. since 2008, several sporadic cases and nosocomial outbreaks associated with high case-fatality have been reported in villages and rural hospitals in the region. | 2011 | 21655310 |
| crimean-congo hemorrhagic fever virus activates endothelial cells. | crimean-congo hemorrhagic fever virus (cchfv) causes viral hemorrhagic fever with high case fatality rates and is geographically widely distributed. . due to the requirement of a biosafety level 4 (bsl-4) laboratory and the lack of an animal model, the knowledge behind the pathogenesis of this virus is limited. crimean-congo hemorrhagic fever (cchf) is characterized by hemorrhage and vascular permeability indicating the involvement of endothelial cells in pathogenesis. the interplay between ecs ... | 2011 | 21632768 |
| inhibition of hazara nairovirus replication by small interfering rnas and their combination with ribavirin. | abstract: background: the genus nairovirus in the family bunyaviridae contains 34 tick-borne viruses classified into seven serogroups. hazara virus (hazv) belongs to the crimean-congo hemorrhagic fever (cchf) serogroup that also includes cchf virus (cchfv) a major pathogen for humans. hazv is an interesting model to study cchfv due to a close serological and phylogenetical relationship and a classification which allows handling in a bsl2 laboratory. nairoviruses are characterized by a tripartite ... | 2011 | 21600011 |
| a case of crimean-congo haemorrhagic fever successfully treated with therapeutic plasma exchange and ribavirin. | crimean-congo haemorrhagic fever (cchf) has become an important problem in certain parts of the world. cytokine storm plays a critical role in the pathogenesis of cchf. early diagnosis, supportive therapy and invasive monitoring are the cornerstones of the management of cchf. ribavirin is active against cchf virus in vitro, however, evidence of clinical efficacy is still conflicting. here, we describe a patient successfully recovered from cchf with a combination of plasma exchange and ribavirin ... | 2011 | 21565949 |
| epidemiological survey of crimean-congo hemorrhagic fever virus in yunnan, china, 2008. | objectives: we aimed to determine the seroprevalence of crimean-congo hemorrhagic fever virus (cchfv) infection in yunnan province, china. materials and methods: one thousand six hundred and fifty-seven human serum samples and 1280 ticks (hyalomma asiaticum) were collected from five counties (menglian, menghai, lancang, mengla, and ximeng). serum samples were analyzed independently by indirect immunofluorescence assay and western blotting to detected cchfv antibody. the ticks were examined by re ... | 2011 | 21546303 |
| [crimean-congo hemorrhagic fever: basics for general practitioners]. | crimean-congo hemorrhagic fever (cchf) is a tick-borne disease described in more than 30 countries in europe, asia and africa. the causative agent is the crimean-congo hemorrhagic fever virus (cchfv) that is a member of the genus nairovirus of the family bunyaviridae. cchfv that is characterized by a high genetic variability is transmitted to humans by tick bites or contact with fluids from an infected individual or animal. the initial symptoms of cchf are nonspecific and gradually progress to a ... | 2010 | 21520641 |
| crimean-congo hemorrhagic fever disease due to tick bite with very long incubation periods. | background: crimean-congo hemorrhagic fever (cchf) is a zoonotic viral disease with a high mortality rate, and is one of the viral hemorrhagic fever syndromes. the average mortality rate of cchf is 3-30%. research indicates that the longest incubation period after a tick bite is 12 days in cchf disease. however, in clinical practice, we encounter patients with cchf as a result of tick bites with much longer incubation periods (max. 53 days) than those reported in the literature. we present herei ... | 2011 | 21511509 |
| structural characterization of the crimean-congo hemorrhagic fever virus gn tail provides insight into virus assembly. | the rna virus that causes the crimean congo hemorrhagic fever (cchf) is a tick-borne pathogen of the nairovirus genus, family bunyaviridae. unlike many zoonotic viruses that are only passed between animals and humans, the cchf virus can also be transmitted from human to human with an overall mortality rate approaching 30%. currently, there are no atomic structures for any cchf virus proteins nor for any nairovirus proteins. a critical component of the virus is the envelope gn glycoprotein, which ... | 2011 | 21507948 |
| [differentiation of genetic variants of crimean-congo hemorrhagic fever virus]. | the paper describes a simple, rapid screening of samples potentially containing crimean-congo hemorrhagic fever (cchf) virus strains, by applying the restriction analysis of amplicones, for the differentiation of cchf virus genotypes that are characteristic of europe from virus biovariants uncharacteristic of this area, this technique requiring no sequence at the first stage. for this screening, the authors propose to use the pcr fragment of cchf l segment that comprises a variable region, as we ... | 2011 | 21427952 |
| crimean-congo hemorrhagic fever: current and future prospects of vaccines and therapies. | crimean-congo hemorrhagic fever (cchf) is a tick-borne disease caused by cchf virus (cchfv), a nairovirus in the family bunyaviridae. cchf occurs sporadically in a number of countries in asia, the middle east, southeastern europe and africa. patients may develop subclinical to severe hemorrhagic disease, with fatal outcomes in a substantial percentage of cases. transmission usually occurs through contact with viremic livestock or patients or bites by infected ticks. the number of reported cases ... | 2011 | 21362441 |
| quantitative analysis of particles, genomes and infectious particles in supernatants of haemorrhagic fever virus cell cultures. | information on the replication of viral haemorrhagic fever viruses is not readily available and has never been analysed in a comparative approach. here, we compared the cell culture growth characteristics of haemorrhagic fever viruses (hfv), of the arenaviridae, filoviridae, bunyaviridae, and flavivridae virus families by performing quantitative analysis of cell culture supernatants by (i) electron microscopy for the quantification of virus particles, (ii) quantitative real time pcr for the quan ... | 2011 | 21349180 |
| species diversity of ixodid ticks feeding on humans in amasya, turkey: seasonal abundance and presence of crimean-congo hemorrhagic fever virus. | ticks (acari:ixodidae) are important pests transmitting tick-borne diseases such as crimean-congo hemorrhagic fever (cchf) to humans. between 2002 and 2009, numerous cchf cases were reported in turkey, including amasya province. in the current study, species diversity, seasonal abundance of ticks, and presence of cchf virus (cchfv) in ticks infesting humans in several districts of amasya province were determined. in the survey, a total of 2,528 ixodid ticks were collected from humans with tick b ... | 2011 | 21337953 |
| newly recognized mosquito-associated viruses in mainland china, in the last two decades. | there are four principal arboviruses in mainland china. two kinds of them are mosquito-borne viruses, namely japanese encephalitis virus and dengue virus, which lead to japanese encephalitis, and dengue fever/dengue hemorrhagic fever respectively; the other two are tick-borne viruses, namely tick-borne encephalitis virus and crimean-congo hemorrhagic fever virus (also known as xinjiang hemorrhagic fever virus), which contribute to tick-borne encephalitis and xinjiang hemorrhagic fever respective ... | 2011 | 21314994 |
| molecular basis for ubiquitin and isg15 cross-reactivity in viral ovarian tumor domains. | crimean congo hemorrhagic fever virus (cchfv) is a deadly human pathogen that evades innate immune responses by efficiently interfering with antiviral signaling pathways mediated by nf-?b, irf3, and ifna/ß. these pathways rely on protein ubiquitination for their activation, and one outcome is the modification of proteins with the ubiquitin (ub)-like modifier interferon-stimulated gene (isg)15. cchfv and related viruses encode a deubiquitinase (dub) of the ovarian tumor (otu) family, which unlike ... | 2011 | 21266548 |
| structural basis for the removal of ubiquitin and interferon-stimulated gene 15 by a viral ovarian tumor domain-containing protease. | the attachment of ubiquitin (ub) and the ub-like (ubl) molecule interferon-stimulated gene 15 (isg15) to cellular proteins mediates important innate antiviral responses. ovarian tumor (otu) domain proteases from nairoviruses and arteriviruses were recently found to remove these molecules from host proteins, which inhibits ub and isg15-dependent antiviral pathways. this contrasts with the ub-specific activity of known eukaryotic otu-domain proteases. here we describe crystal structures of a viral ... | 2011 | 21245344 |
| structural analysis of a viral ovarian tumor domain protease from the crimean-congo hemorrhagic fever virus in complex with covalently bonded ubiquitin. | crimean-congo hemorrhagic fever (cchf) virus is a tick-borne, negative-sense, single-stranded rna [ssrna(-)] nairovirus that produces fever, prostration, and severe hemorrhages in humans. with fatality rates for cchf ranging up to 70% based on several factors, cchf is considered a dangerous emerging disease. originally identified in the former soviet union and the congo, cchf has rapidly spread across large sections of europe, asia, and africa. recent reports have identified a viral homologue of ... | 2011 | 21228232 |
| crimean-congo hemorrhagic fever virus, northeastern greece. | 2011 | 21192882 | |
| molecular epidemiology of crimean- congo hemorrhagic fever virus genome isolated from ticks of hamadan province of iran. | crimean-congo hemorrhagic fever (cchf) virus is a tick-borne member of the genus nairovirus, family bunyaviridae. cchfv has been isolated from at least 31 different tick species. the virus is transmitted through the bite of an infected tick, or by direct contact with cchfv-infected patients or the products of infected livestock. this study was undertaken to study the genetic relationship and distribution of cchfv in the tick population of hamadan province of iran. | 2010 | 21178213 |
| phylogenetic analysis in a recent controlled outbreak of crimean-congo haemorrhagic fever in the south of iran, december 2008. | crimean-congo haemorrhagic fever (cchf) is a viral zoonotic disease with a high mortality rate in humans. the cchf virus is transmitted to humans through the bite of ixodid ticks or contact with blood or tissues of cchf patients or infected livestock. in december 2008, a re-emerging outbreak of cchf occurred in the southern part of iran. five people were hospitalised with sudden fever and haemorrhaging, and cchf was confirmed by rt-pcr and serological assays. one of the cases had a fulminant cou ... | 2010 | 21144440 |
| the bulgarian vaccine crimean-congo haemorrhagic fever virus strain. | the crimean-congo haemorrhagic fever virus (cchfv) is a 3-segmented rna virus, which causes disease with a high fatality rate in humans. an inactivated suckling mouse brain-derived vaccine is used in bulgaria for protection against cchf. strain v42/81 is currently used for the vaccine preparation. as the m-rna segment plays a major role in the immune response, the full-length m segment sequence of the v42/81 strain was characterized. a great genetic diversity was observed among cchfv strains. in ... | 2010 | 21142621 |
| induction of caspase activation and cleavage of the viral nucleocapsid protein in different cell types during crimean-congo hemorrhagic fever virus infection. | regulation of apoptosis during infection has been observed for several viral pathogens. programmed cell death and regulation of apoptosis in response to a viral infection are important factors for host or virus survival. it is not known whether crimean-congo hemorrhagic fever virus (cchfv) infection regulates the apoptosis process in vitro. this study for the first time suggests that cchfv induces apoptosis, which may be dependent on caspase-3 activation. this study also shows that the coding se ... | 2010 | 21123175 |
| detection of crimean-congo hemorrhagic fever, hanta, and sandfly fever viruses by real-time rt-pcr. | the development of sensitive and specific nucleic acid diagnostic assays for viral pathogens is essential for proper medical intervention. this chapter describes four fluorescence-based pcr assays to detect the crimean-congo hemorrhagic fever (cchfv), andes (andv), hantaan (hanv), and sandfly fever sicilian (sfsv) viruses. these assays are based on species-specific hydrolysis probes targeting the nucleocapsid protein gene for cchfv and sfsv and the glycoprotein gene for andv and hanv. all four a ... | 2011 | 21116810 |
| effect of oral ribavirin treatment on the viral load and disease progression in crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) is a lethal hemorrhagic disease. there is currently no specific antiviral therapy for cchf approved for use in humans. in this study we aimed to investigate the effect of oral ribavirin treatment on the viral load and disease progression in cchf. | 2010 | 21106428 |
| complete sequence and phylogenetic characterisation of crimean-congo hemorrhagic fever virus from afghanistan. | 2010 | 21035389 | |
| crimean-congo hemorrhagic fever in european part of turkey: genetic analysis of the virus strains from ticks and a seroepidemiological study in humans. | abstract a survey of ticks from domestic ruminants, together with a serosurvey in humans was conducted in thrace (northwestern turkey) to evaluate the prevalence of crimean-congo hemorrhagic fever virus (cchfv) in ticks and humans. more prevalent ticks were hyalomma marginatum, hyalomma aegyptium, rhipicephalus bursa, and rhipicephalus (boophilus) annulatus, with low numbers of dermacentor marginatus, rhipicephalus sanguineus group, and ixodes ricinus. no differences in the tick faunal compositi ... | 2010 | 21028961 |
| serial expression of the truncated fragments of the nucleocapsid protein of cchfv and identification of the epitope region. | the crimean-congo hemorrhagic fever virus (cchfv) is a geographically widespread fatal pathogen. identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of cchfv infections. in this study, expression vectors carrying series truncated fragments of the np (nucleocapsid protein) gene from the s fragment of cchfv strain yl04057 were constructed. the recombinant proteins were expressed in e.coli and purified for detection. the antigenic of the tru ... | 2010 | 20960283 |
| the first crimean-congo hemorrhagic fever case in the winter season from turkey. | turkey is one of the countries in which crimean-congo hemorrhagic fever is frequently seen and most of the cases are reported between april and august. we describe the first case of crimean-congo hemorrhagic fever in the winter season, when hyalomma tick activity is absent, from turkey. | 2010 | 20948224 |
| [a case of crimean-congo hemorrhagic fever in the anapa district, krasnodar territory]. | the paper gives information on the first case of crimean-congo hemorrhagic fever detected in a female resident of the anapa district, krasnodar territory, in 2005 in the past 57 years. | 2010 | 20886712 |
| virus and infections 2010 - bit's first world congress. | the world congress of virus and infections, held in busan, south korea, included topics reviewing the field of zoonoses. this conference report highlights selected presentations on surveillance, epidemiology and measures for the control and prevention of zoonotic diseases. topics discussed include human factors influencing zoonoses, the molecular epidemiology of crimean-congo hemorrhagic fever, the emerging nipah virus, and the re-emergence of cowpox virus. | 2010 | 20878585 |
| interview with dr. anthony r. fooks. interview by vicki glaser. | 2010 | 20854027 | |
| interventions against west nile virus, rift valley fever virus, and crimean-congo hemorrhagic fever virus: where are we? | arbo-zoonet is an international network financed by the european commission's seventh framework program. the major goal of this initiative is capacity building for the control of emerging viral vector-borne zoonotic diseases, with a clear focus on west nile virus, rift valley fever virus, and crimean-congo hemorrhagic fever virus. to evaluate the status quo of control measures against these viruses, an arbo-zoonet meeting was held in istanbul, turkey, from 19 to 20 november 2009. the symposium c ... | 2010 | 20854026 |
| crimean-congo hemorrhagic fever in a dengue-endemic region: lessons for the future. | crimean-congo hemorrhagic fever and dengue hemorrhagic fever are endemic in pakistan. however, the overlap of geographic distribution and early clinical features between the two conditions make a reliable diagnosis difficult in the initial stage of illness. a 16-year-old boy presented with a history of hematemesis and high-grade fever. a preliminary diagnosis of dengue hemorrhagic fever was made and supportive treatment was instituted; however, the patient continued to deteriorate clinically. de ... | 2010 | 20818095 |
| crimean-congo haemorrhagic fever in eurasia. | crimean-congo haemorrhagic fever (cchf) is a tick-borne infectious disease characterized by fever, malaise, headache, nausea, vomiting, diarrhoea, sore throat, muscle aches, haemorrhage and thrombocytopenia. it is a challenge for the population of endemic rural areas, and for healthcare workers, and carries a considerable mortality. the disease is widely distributed in africa, europe and asia, and has become a serious threat to public health in eurasia. the intention of this review is to summari ... | 2010 | 20810253 |
| nosocomial outbreak of crimean-congo haemorrhagic fever. | summarywe report a nosocomial outbreak of crimean-congo haemorrhagic fever (cchf) that affected six patients in june 2009 in ghaem hospital, mashhad, iran, apparently related to one index case. the last four cases were healthcare workers. infection was spread by percutaneous exposure to two cases, and probably by direct contact with blood, clothes and sheets, to three others. the diagnosis in the two fatal cases was not confirmed virologically. the diagnosis in four cases who survived was confir ... | 2011 | 20800007 |
| pathogenesis and immune response of crimean-congo hemorrhagic fever virus in a stat-1 knockout mouse model. | tick-borne crimean-congo hemorrhagic fever virus (cchfv) causes a severe hemorrhagic syndrome in humans but not in its vertebrate animal hosts. the pathogenesis of the disease is largely not understood due to the lack of an animal model. laboratory animals typically show no overt signs of disease. here, we describe a new small-animal model to study cchfv pathogenesis that manifests clinical disease, similar to that seen in humans, without adaptation of the virus to the host. our studies revealed ... | 2010 | 20739514 |
| current treatment of crimean-congo hemorrhagic fever in children. | crimean-congo hemorrhagic fever (cchf) is a fatal viral hemorrhagic fever and is usually transmitted to humans by tick bite, or exposure to infected blood or tissues of infected livestock or humans. although children can be infected with the cchf virus, infection is unusual in the younger age group. early diagnosis and treatment of cchf infection is critical to the survival of patients and the control of the disease. in this article, we underline current therapeutic approaches to cchf infection ... | 2010 | 20695747 |
| crimean-congo hemorrhagic fever in man, republic of georgia, 2009. | 2010 | 20678341 | |
| determination of serum adenosine deaminase and xanthine oxidase levels in patients with crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever is an acute viral hemorrhagic fever with a high mortality rate. despite increasing knowledge about hemorrhagic fever viruses, little is known about the pathogenesis of crimean-congo hemorrhagic fever. in this study, we measured serum adenosine deaminase and xanthine oxidase levels in crimean-congo hemorrhagic fever patients. | 2010 | 20668627 |
| evidence of vascular endothelial damage in crimean-congo hemorrhagic fever. | endothelial infection has an important role in the pathogenesis of crimean-congo hemorrhagic fever (cchf). in this study, we investigated the causes of vascular endothelial damage in patients with cchf. | 2010 | 20627646 |
| ixodid ticks (acari: ixodidae) infesting humans in tokat province of turkey: species diversity and seasonal activity. | ixodid ticks (acari: ixodidae) are the major vectors of pathogens threatening animal and human health. tokat province, turkey, is a suitable habitat for extended tick activity with its moderate climate and vegetation. in the present study, we surveyed humans visiting health centers to determine the species diversity, geographical distribution, and seasonal activity of ixodid ticks infesting them. out of 5,999 adult ticks collected from humans from april to september, 2008, 800 ticks were identif ... | 2010 | 20618665 |
| a simple-probe real-time pcr assay for genotyping reassorted and non-reassorted isolates of crimean-congo hemorrhagic fever virus in southern africa. | crimean-congo hemorrhagic fever (cchf) is a tick-borne viral zoonosis distributed widely in africa, asia, russia and the balkans. occurrence of segment reassortment has been established and may be associated with pathogenicity. the ability to distinguish between an infection with a reassortant and a non-reassortant variant may have prognostic value. in this study the use of simple-probe technology and real-time pcr was investigated to detect rapidly cchf viral nucleic acid and to distinguish bet ... | 2010 | 20599559 |
| crimean-congo haemorrhagic fever presenting as epididymo-orchitis. | crimean-congo haemorrhagic fever (cchf) is a potentially fatal disease caused by a tick-borne virus. | 2010 | 20598630 |
| early serodiagnosis of acute human crimean-congo hemorrhagic fever virus infections by novel capture assays. | 2010 | 20541967 | |
| crimean-congo hemorrhagic fever: risk for emergence of new endemic foci in europe? | during recent years, new foci of crimean-congo hemorrhagic fever (cchf) have emerged in several balkan countries, southwest russia, and turkey. starting in 2002, turkey experiences the largest ever recorded outbreak with more than 2500 cases. potential reasons for the emergence or re-emergence of cchf include climate changes which may have a significant impact on the reproduction rate of the vector hyalomma ticks, as well as anthropogenic factors (e.g. changes in agricultural and hunting activit ... | 2010 | 20541133 |
| comparison of antiviral activity of recombinant and natural interferons against crimean-congo hemorrhagic fever virus. | as a first line of defence against a virus infection, mammalian cells elicit an innate immune response, characterized by secretion of type i interferons (ifn) and up-regulation of interferon stimulated genes (isgs). we have previously included crimean congo hemorrhagic fever virus (cchfv) in the list of type i ifn-sensitive viruses. in this in vitro study, we have compared the antiviral activity of two recombinant ifn-alpha preparations (roferon a and intron a) with a natural ifn-alpha produced ... | 2010 | 20517491 |
| epidemiological, serological and herd immunity of crimean-congo haemorrhagic fever in kosovo. | crimean-congo haemorrhagic fever (cchf) is primarily a zoonotic disease, mostly present as sporadic cases, but outbreaks also occur, especially in the family. disease as endemic form is presents in some countries of africa, europe and asia. in 2001, outbreak of cchf was registered in kosova, albania, pakistan, iran, and south africa. goal of the research was to establish a pattern of the disease, its natural flow and herd immunity. for this purpose we used epidemiological methods, laboratory con ... | 2010 | 20514773 |
| interacting roles of immune mechanisms and viral load in the pathogenesis of crimean-congo hemorrhagic fever. | until now, the pathogenesis of crimean-congo hemorrhagic fever (cchf) has not been well described. however, it has been hypothesized that it could be a result of the direct injury of virus-infected tissues in combination with the indirect effects of host immune responses, including cytokines. to shed more light on the role of viral load and cytokines, differential influences of cchf virus (cchfv) rna load, antibody response, and cytokine production on severity and outcome of the disease were stu ... | 2010 | 20484568 |
| [phosphan microplate technology-based microarray for the determination of igg antibodies against west nile and crimean-congo hemorrhagic fever viruses]. | the paper demonstrates it possible to work out a phosphorescence analysis (phosphan) microplate technology-based microarray for concurrently examining human sera and detection of their specific igg antibodies against two heterological west nile and crimean-congo hemorrhagic fever viruses. the sensitivity and specificity of the microarray were comparable with those of enzyme immunoassay with separate sample testing. the advantages of phosphan were associated with the microplate format of an immun ... | 2010 | 20455465 |
| [evaluation of pcr and elisa-igm results in the laboratory diagnosis of crimean-congo haemorrhagic fever cases in 2008 in turkey]. | crimean-congo hemorrhagic fever (cchf) is a fatal zoonotic viral haemorrhagic infection described in africa, asia, eastern europe, and the middle east. cchf virus (cchfv) classified in bunyaviridae family and nairovirus genus, is transmitted to humans by tick (hyalomma and ixodid) bites and human to human transmission may occur by direct contact with blood or other infected tissues. the disease became endemic and a public health problem since 2002 outbreak in turkey. the specific laboratory diag ... | 2010 | 20455399 |
| crimean-congo hemorrhagic fever in children. | crimean-congo hemorrhagic fever (cchf) virus causes a severe disease in humans with a mortality up to 30%. in turkey there has been an increase in the number of cases during years since 2002. humans of all ages living in endemic areas,especially those who are working as shepherds and toddlers, have high risk of acquiring cchf. | 2010 | 20444644 |
| nosocomial outbreak of crimean-congo hemorrhagic fever, sudan. | to confirm the presence of crimean-congo hemorrhagic fever in sudan, we tested serum of 8 patients with hemorrhagic fever in a rural hospital in 2008. reverse transcription-pcr identified crimean-congo hemorrhagic fever virus. its identification as group iii lineage indicated links to virus strains from south africa, mauritania, and nigeria. | 2010 | 20409377 |
| cutaneous manifestations of crimean-congo haemorrhagic fever: morbilliform eruptions may reflect a favorable outcome and not low platelet levels. | 2010 | 20406726 | |
| a serological survey of crimean-congo haemorrhagic fever in animals in the sharkia governorate of egypt. | a surveillance survey was conducted over a period of 12 months between september 2004 and august 2005 by the tropical medicine department of zagazig university in collaboration with naval medical research unit no. 3 (namru-3), egypt, to evaluate the role of ruminants as a reservoir host for crimean-congo haemorrhagic fever virus (cchfv). a total of 1,022 serum samples from 313 cattle, 264 water buffalo (bubalus bubalis), 270 sheep and 175 goats were included in the survey. all samples were colle ... | 2008 | 20405447 |
| a perspective on emerging mosquito and phlebotomine-borne diseases in europe. | 2010 | 20403312 | |
| an antigenic investigation of crimean-congo hemorrhagic fever virus (cchfv) in hard ticks from provinces in northern turkey. | crimean-congo hemorrhagic fever virus threatens human health. exposure of the infected tick is a strong risk factor for human disease. in this study, the hard ticks collected from a variety of mammalian species (cattle, sheep, goat, and buffalo) and a turtle in either coastal or inland black sea region of turkey were surveyed for the presence of antigen from crimean-congo hemorrhagic fever virus (cchfv). cchfv antigen was found in 46 of 421 tick pools (10.92%). positivity rates for the provinces ... | 2010 | 20401757 |
| erythema nodosum in the course of crimean-congo haemorrhagic fever. | crimean-congo haemorrhagic fever is an emerging zoonosis with a wide geographic distribution and protean clinical manifestations. the main pathogenesis of this infection has not been elucidated. erythema nodosum is an immunologically-mediated dermatologic disease and the most common form of panniculitis that has been associated with many infections and inflammatory disorders. we describe a case of crimean-congo haemorrhagic fever with severe bleeding diathesis and hepatic failure that was succes ... | 2010 | 20305115 |
| evaluation of clinical and laboratory predictors of fatality in patients with crimean-congo haemorrhagic fever in a tertiary care hospital in turkey. | the fatality rate of crimean-congo haemorrhagic fever (cchf) disease has been reported as 5.4-80%. in this prospective study our aim was to evaluate the clinical and laboratory predictors of fatality in patients with cchf. among probable cchf patients admitted to our clinic between 2005 and 2008, patients with positive igm antibodies and/or polymerase chain reaction for cchf virus were included in the study. to determine the predictors of fatality, we compared epidemiological, clinical and labor ... | 2010 | 20166862 |
| crimean-congo hemorrhagic fever virus infection is lethal for adult type i interferon receptor-knockout mice. | crimean-congo hemorrhagic fever virus (cchfv) poses a great threat to public health due to its high mortality, transmission and geographical distribution. to date, there is no vaccine or specific treatment available and the knowledge regarding its pathogenesis is highly limited. using a small-animal model system, this study showed that adult mice missing the type i interferon (ifn) receptor (ifnar(-/-)) were susceptible to cchfv and developed an acute disease with fatal outcome. in contrast, inf ... | 2010 | 20164263 |
| molecular detection of crimean-congo haemorrhagic fever virus (cchfv) but not west nile virus (wnv) in hard ticks from provinces in northern turkey. | in this study, the hard ticks collected from a variety of mammalian species (cattle, sheep, goat, buffalo) and a turtle in either coastal or inland black sea region of turkey were surveyed for the presence of rna from crimean-congo haemorrhagic fever virus (cchfv) and west nile virus (wnv). no wnv genomic rna was detected in any tick sample. however, cchfv rna was found in 29 of 421 tick pools (6.88%). positivity rates for the provinces varied and were as follows: samsun 4.38%; ordu 4.34%; gires ... | 2010 | 20163578 |
| serum nitric oxide levels in patients with crimean-congo haemorrhagic fever. | crimean-congo haemorrhagic fever (cchf) is an acute disease affecting multiple organ systems and is characterized by fever and haemorrhages. the pathogenesis of cchf has not been well described. nitric oxide (no) is an important regulator of a number of different biological processes and can participate in antimicrobial defence. in this study, we measured the level of no in the serum of patients with cchf and healthy controls to define the possible role of no in the control of infection. sixty-t ... | 2010 | 20095935 |
| [epidemic situation on kongo-crimean hemorrhagic fever in south federal district of russia]. | 2009 | 20095423 | |
| [two crimean-congo hemorrhagic fever cases without history of tick contact from ankara region]. | crimean-congo hemorrhagic fever (cchf) is a tick-borne viral disease presenting with flu-like symptoms, fever, hemorrhage and petechia. the virus (cchfv) is a member of the nairovirus genera of bunyaviridae family and can be transmitted to humans by hyalomma tick-bite, by exposure to infected blood and fomites of patient with cchf or contact with animal tissue in viremic phase. in this study we present two cases with cchf but without history of tick bite or exposure to infected fomites, even not ... | 2009 | 20084924 |
| molecular epidemiology of crimean-congo hemorrhagic fever virus in turkey: occurrence of local topotype. | the goal of this study was to investigate the molecular epidemiology of crimean-congo hemorrhagic fever virus (cchfv) in turkey. the study was performed on a total of 48 confirmed human cchf cases from 2006 to 2008. the majority of the cchf viral strains in turkey were found to belong to the european lineage. local cchf viral strains are grouped into two main clusters, which can be further divided into two sub-groups. we also identified an ap92-like virus causing clinical disease in corum (a mid ... | 2010 | 20079776 |
| high-dose methylprednisolone in children with crimean-congo haemorrhagic fever. | treatment options for crimean-congo haemorrhagic fever (cchf) are limited and based on general supportive managements. thrombocytopenia is the major risk factor of cchf. we report our experience with high-dose methylprednisolone (hdmp). this study included five patients with cchf. patients were given hdmp if there were findings compatible with virus-associated haemophagocytic syndrome and the effects of hdmp were evaluated. following this, hdmp fever subsided and platelet counts increased within ... | 2010 | 20075424 |
| ancient common ancestry of crimean-congo hemorrhagic fever virus. | crimean-congo hemorrhagic fever (cchf) is a tick-borne rna virus responsible for outbreaks of severe hemorrhagic fever in humans. although cchf was first detected in the 1940s, high levels of genomic diversity argue against a recent origin. here, bayesian coalescent analyses were used to estimate the rate of evolution and relative age of the virus. a total of 43 s, 34 m, and 23 l segment sequences from samples collected between 1956 and 2005 were analyzed from across the broad geographic range o ... | 2010 | 20074652 |
| [novel diagnostic kit on the basis of stained polymeric carriers for epidemiologic surveillance in natural foci of crimean-congo hemorrhagic fever]. | to study diagnostic value of developed antigenic polymeric diagnostic kit for epidemiologic surveillance in natural foci of crimean-congo hemorrhagic fever (cchf) in south federal district. | 2009 | 20066777 |
| the feasibility of developing a risk assessment for the impact of climate change on the emergence of crimean-congo haemorrhagic fever in livestock in europe: a review. | crimean-congo haemorrhagic fever virus (cchfv) is one of the most widespread of all medically important arboviruses with ticks of the hyalomma spp. serving as the main vectors. infection of livestock by cchfv serves as a route of exposure to humans, as a reservoir of disease and as a route of importation. this study discusses the pathways and data requirements for a qualitative risk assessment for the emergence of cchfv in livestock in europe. a risk map approach is proposed based on layers that ... | 2010 | 20015209 |
| a clear and present danger: tick-borne diseases in europe. | ticks can transmit a variety of viruses, bacteria or parasites that can cause serious infections or conditions in humans and animals. while tick-borne diseases are becoming an increasing and serious problem in europe, tick-borne diseases are also responsible for major depressions in livestock production and mortality in sub-saharan africa, latin america and asia. this review will focus on the most important circulating tick-transmitted pathogens in europe (borrelia spp., anaplasma phagocytophilu ... | 2010 | 20014900 |
| crimean-congo hemorrhagic fever in iran and neighboring countries. | crimean-congo hemorrhagic fever (cchf) is a zoonotic viral disease that is asymptomatic in infected livestock, but a serious threat to humans. human infections begin with nonspecific febrile symptoms, but progress to a serious hemorrhagic syndrome with a case fatality rate of 2-50%. although the causative virus is often transmitted by ticks, livestock-to-human and human-to-human transmissions also occur. the disease is one of the most widely distributed viral hemorrhagic fevers occurring in afri ... | 2010 | 20006541 |
| a newly identified crimean-congo hemorrhagic fever virus strain in turkey. | crimean-congo hemorrhagic fever (cchf) is a fatal viral disease that occurs in approximately 30 countries. it has the most extensive geographic range among the tick-borne viruses that affect human health. recently, a 6-year-old boy presented with complaints of fever, fatigue, and loss of appetite. he revealed a history of tick bite in rural istanbul three days prior to presentation. a hyperemia was detected at the site of the tick bite. laboratory tests showed that alanine aminotransferase, aspa ... | 2010 | 20005760 |
| the efficacy of ribavirin in the treatment of crimean-congo hemorrhagic fever in eastern black sea region in turkey. | the efficiency of ribavirin for treatment of crimean-congo hemorrhagic fever (cchf) is unknown. in the literature, prospective randomized studies investigating the efficacy of ribavirin are not found. | 2010 | 19962342 |
| a survey of crimean-congo haemorrhagic fever in livestock and ticks in ardabil province, iran during 2004-2005. | crimean-congo haemorrhagic fever (cchf) is a viral haemorrhagic fever caused by the cchf virus. it is mainly transmitted to humans and animals by ticks. in recent y, large numbers of livestock have been transported across the border areas of ardabil province resulting in an outbreak of cchf in the adjacent districts. a comprehensive study was carried out to assess the epidemiological aspects of the disease in this province. in the study area, 130 ticks were collected from randomly selected villa ... | 2010 | 19958240 |
| the complete genome analysis of crimean-congo hemorrhagic fever virus isolated in turkey. | crimean-congo hemorrhagic fever virus (cchfv) is the causative agent of a tick-borne disease with high mortality rates in humans. the distribution of cchfv includes over 30 countries in asia, the middle east, southeastern europe, and africa. it was first recognized in turkey in 2002, with an increasing number of cases reported between 2002 and 2009. recent analysis of complete genome sequences of cchfv isolates has revealed that the genomic plasticity of the virus is surprisingly high for an art ... | 2010 | 19941917 |
| contact tracing and serosurvey among healthcare workers exposed to crimean-congo haemorrhagic fever in greece. | we investigated the probability of nosocomial transmission of crimean-congo haemorrhagic fever (cchf) virus among healthcare workers (hcws) who cared for the first cchf case in greece. specific igm and/or igg antibodies against cchf virus were not detected in the 21 hcws studied. although person-to-person transmission did not take place, education of hcws about the modes of cchf virus transmission and appropriate infection control measures is needed in order to avoid future nosocomial cases. | 2009 | 19922073 |