Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| human cytomegalovirus secretome contains factors that induce angiogenesis and wound healing. | human cytomegalovirus (hcmv) is implicated in the acceleration of a number of vascular diseases including transplant vascular sclerosis (tvs), the lesion associated with chronic rejection (cr) of solid organ transplants. although the virus persists in the allograft throughout the course of disease, few cells are directly infected by cmv. this observation is in contrast to the global effects that cmv has on the acceleration of tvs/cr, suggesting that cmv infection indirectly promotes the vascular ... | 2008 | 18448536 |
| tnf receptor independent activation of the cytomegalovirus major immediate early enhancer in response to transplantation. | reactivation of latent human cytomegalovirus (hcmv) infection is a significant risk factor for long term allograft dysfunction. the molecular pathways involved in reactivation of latent virus have not been identified. previous studies suggested that tumor necrosis factor (tnf) -mediated activation of nuclear factor of kappa light polypeptide gene enhancer in b-cells (nf-kappa b) leading to transcriptional reactivation of viral immediate early (ie) gene expression might be important in transplant ... | 2008 | 18408586 |
| comparison of nested polymerase chain reaction (pcr), real-time pcr and viral culture for the detection of cytomegalovirus in subgingival samples. | the purpose of this study was to compare nested polymerase chain reaction (pcr), real-time pcr, and shell vial for the detection of human cytomegalovirus (hcmv) in subgingival samples in periodontitis patients. | 2008 | 18402611 |
| human cytomegalovirus dnaemia and preemptive treatment of cmv infection in children after hematopoietic stem-cell transplantation: is any question settled? | 2008 | 18398062 | |
| multimerization of tegument protein pp28 within the assembly compartment is required for cytoplasmic envelopment of human cytomegalovirus. | human cytomegalovirus (hcmv) ul99-encoded pp28 is an essential tegument protein required for envelopment and production of infectious virus. nonenveloped virions accumulate in the cytoplasm of cells infected with recombinant viruses with the ul99 gene deleted. previous results have suggested that a key function of pp28 in the envelopment of infectious hcmv is expressed after the protein localizes in the assembly compartment (ac). in this study, we investigated the potential role of pp28 multimer ... | 2008 | 18385241 |
| human cmv infection of endothelial cells induces an angiogenic response through viral binding to egf receptor and beta1 and beta3 integrins. | human cytomegalovirus (hcmv) infection is associated with atherosclerosis, transplant vascular sclerosis, and coronary restenosis. a common theme in these vascular diseases is an increased rate of angiogenesis. angiogenesis is a complex biological process mediated by endothelial cell (ec) proliferation, migration, and morphogenesis. although angiogenesis is a normal process in the host, its dysregulation, after viral infection or injury to the vessel wall, is associated with plaque development i ... | 2008 | 18375753 |
| human cytomegalovirus infection induces a rapid and sustained change in the expression of nk cell receptors on cd8+ t cells. | the cd8(+) t cell compartment of human cmv-seropositive individuals characteristically contains a high proportion of cells that express nk cell receptors (nkrs) which may contribute to the surveillance of virus-infected cells. to test whether this enhanced expression is a direct and immediate result of cmv infection, we used dna microarrays to analyze putative changes in the rna expression level of 39 nkrs in cmv-specific cd8(+) t cells of renal transplant recipients experiencing primary cmv inf ... | 2008 | 18354177 |
| nuclear localization sequences in cytomegalovirus capsid assembly proteins (ul80 proteins) are required for virus production: inactivating nls1, nls2, or both affects replication to strikingly different extents. | scaffolding proteins of spherical prokaryotic and eukaryotic viruses have critical roles in capsid assembly. the primary scaffolding components of cytomegalovirus, called the assembly protein precursor (pap, pul80.5) and the maturational protease precursor (ppr, pul80a), contain two nuclear localization sequences (nls1 and nls2), at least one of which is required in coexpression experiments to translocate the major capsid protein (mcp, pul85) into the nucleus. in the work reported here, we have ... | 2008 | 18353959 |
| induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production. | human cytomegalovirus (cmv) infection is a major cause of morbidity in immunosuppressed individuals, and congenital cmv infection is a leading cause of birth defects in newborns. infection with pathogenic viral strains alters cell-cell and cell-matrix interactions, affecting extracellular matrix remodeling and endothelial cell migration. the multifunctional cytokine transforming growth factor (tgf)-beta1 regulates cell proliferation, differentiation, and extracellular matrix remodeling. secreted ... | 2008 | 18349127 |
| human cytomegalovirus serum neutralizing antibodies block virus infection of endothelial/epithelial cells, but not fibroblasts, early during primary infection. | a panel of human sera exhibited a >or=128-fold higher neutralizing potency against a human cytomegalovirus (hcmv) clinical isolate propagated and tested in endothelial (or epithelial) cells than against the same virus infecting human fibroblasts. in a group of 18 primary infections, the reverse geometric mean titre was in the range of 10-15 in human fibroblasts within the first 3 months after the onset of infection, whereas the endothelial cell infection-neutralizing activity was already present ... | 2008 | 18343825 |
| human cytomegalovirus infection and antiviral immunity in septic patients without canonical immunosuppression. | the human cytomegalovirus (hcmv) is a relevant pathogen in patients with immunosuppressive therapy; however, reactivation and subsequent recurrence occurs also in individuals without canonical immunosuppression as e.g., in patients with septic shock. analyzing the impact of nk- and t-cell immunity on the natural course of hcmv infection in patients with septic shock, it became clear that the presence of hcmv reactive t-helper cells did not prevent the development of reactivation but, the control ... | 2008 | 18327609 |
| functional impairment of cytomegalovirus specific cd8 t cells predicts high-level replication after renal transplantation. | human cytomegalovirus (hcmv) remains an important cause of morbidity after allotransplantation, causing a range of direct effects including hepatitis, pneumonitis, enteritis and retinitis. a dominant risk factor for hcmv disease is high level viral replication in blood but it remains unexplained why only a subset of patients develop such diseases. in this detailed study of 25 renal transplant recipients, we show that functional impairment of hcmv specific cd8 t cells in the production of interfe ... | 2008 | 18325078 |
| phenoxazine derivatives inactivate human cytomegalovirus, herpes simplex virus-1, and herpes simplex virus-2 in vitro. | we examined whether phenoxazine derivatives, 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3h-phenoxazine-3-one (phx-1), 3-amino-1,4alpha-dihydro-4alpha-8-dimethyl-2h-phenoxazine-2-one (phx-2), and 2-amino-phenoxazine-3-one (phx-3) may have antiviral activity against herpes family viruses: human cytomegalovirus (hcmv), herpes simplex virus type 1 (hsv-1), and herpes simplex virus type 2 (hsv-2). the antiviral activity was evaluated by the selectivity index (si), which is the ratio of 50% cytotoxic ... | 2008 | 18319567 |
| recombinant viruses as tools to study human cytomegalovirus immune modulation. | infections with cytomegaloviruses are characterized by an intricate balance between the expression of immunomodulatory viral proteins and antiviral immune defence. for human cytomegalovirus (hcmv), several proteins have been described that interfere with the recognition of infected cells by cd8 t lymphocytes. although the modes of action of these proteins have been elucidated on the molecular level, thus rendering them useful models to understand mhc class i peptide loading and transport, their ... | 2008 | 18301917 |
| impact of clonal competition for peptide-mhc complexes on the cd8+ t-cell repertoire selection in a persistent viral infection. | cd8(+) t-cell responses to persistent viral infections are characterized by the accumulation of an oligoclonal t-cell repertoire and a reduction in the naive t-cell pool. however, the precise mechanism for this phenomenon remains elusive. here we show that human cytomegalovirus (hcmv)-specific cd8(+) t cells recognizing distinct epitopes from the pp65 protein and restricted through an identical hla class i allele (hla b*3508) exhibited either a highly conserved public t-cell repertoire or a priv ... | 2008 | 18270323 |
| differences between mouse and human cytomegalovirus interactions with their respective hosts at immediate early times of the replication cycle. | the promise of the mouse model of cytomegalovirus (cmv) research lies in a cost effective way to obtain significant data in in vivo settings. keeping that promise requires a high degree of equivalency in the human and mouse virus. while genomic structure and many common proteins suggest that this system is appropriate to develop and test concepts in an organismal context, areas of difference have not been evaluated. here we show that the major immediate early protein 1 (ie1) in mcmv binds the re ... | 2008 | 18264718 |
| [clinical value of human cytomegalovirus phosphoprotein 65 in the diagnosis of cytomegalovirus disease]. | to establish a convenient method for diagnosis of active human cytomegalovirus (hcmv) infection in renal transplant recipients. | 2008 | 18250058 |
| development of recombinant canine adenovirus type-2 expressing the gn glycoprotein of seoul virus. | seoul virus glycoprotein gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. in this study, a replication-competent recombinant canine adenovirus type-2 expressing gn was constructed by the in vitro ligation method. the gn expression cassette, including the human cytomegalovirus (hcmv) promoter/enhancer and the sv40 early mrna polyadenylation signal, was cloned into the sspi site of the e3 region which is not ess ... | 2008 | 18249007 |
| update from the 7th annual meeting of the italian society of virology. | the italian society of virology (siv) held a meeting in orvieto (june 24-26, 2007) aimed at promoting interactions and collaborations between scientists in the field of virology. the meeting had an attendance of about 170 virologists from italy. in accordance with the normal format of the siv national meeting the conference transcended all areas of virology. sessions included invited speakers together with selected oral presentation. covered topics included: general virology and viral genetics, ... | 2008 | 18247363 |
| human cytomegalovirus-specific cd4+ and cd8+ t-cell reconstitution in adult allogeneic hematopoietic stem cell transplant recipients and immune control of viral infection. | human cytomegalovirus infection is the most frequent viral complication in patients undergoing hematopoietic stem cell transplantation. we investigated the development of human cytomegalovirus-specific t cells in adult recipients of hematopoietic stem cell transplants. | 2008 | 18245650 |
| proteasome inhibitor mg132 blocks viral dna replication and assembly of human cytomegalovirus. | this study provides evidence that proteasomal activity is required at multiple steps in human cytomegalovirus replication. electron microscopy revealed that no viral particles were assembled in the presence of proteasome inhibitor mg132. immunofluorescence and western blot analyses using mg132 demonstrated that immediate early gene expression was suppressed at low but not high moi. in contrast, expression of late proteins was completely blocked independent of moi. additionally, pulsed-field gel ... | 2008 | 18242185 |
| the immunological burden of human cytomegalovirus infection. | cytomegalovirus (cmv) is a persistent dna virus that has evolved with humans to establish a finely balanced host-virus relationship. this balance is maintained by host immune surveillance since deficiencies in these processes can result in life-threatening disease, as observed in immunologically immature neonates and pharmacologically immunosuppressed transplant recipients. both t cells and natural killer cells are intimately involved in maintaining asymptomatic infection by specific and non-spe ... | 2007 | 18219760 |
| immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection. | human cytomegalovirus (hcmv) establishes a latent infection in hematopoietic cells, from which it can reactivate to cause significant disease in immunocompromised individuals. hcmv expresses a functional homolog of the immunosuppressive cytokine interleukin-10 (termed cmvil-10), and alternate splicing of the cmvil-10 transcript results in expression of a latency-associated cmvil-10 transcript (lacmvil-10). to determine whether lacmvil-10 encodes immunosuppressive functions, recombinant lacmvil-1 ... | 2008 | 18216121 |
| the full-length protein encoded by human cytomegalovirus gene ul117 is required for the proper maturation of viral replication compartments. | previously, two large-scale mutagenic analyses showed that mutations in the human cytomegalovirus (hcmv) gene ul117 resulted in a defect in virus growth in fibroblasts. early transcriptional analyses have revealed several mrnas from the ul119-ul115 region; however, specific transcripts encoding ul117-related proteins have not been identified. in this study, we identified two novel transcripts arising from the ul117 gene locus, and we reported that the ul117 open reading frame encoded the full-le ... | 2008 | 18216115 |
| validation of a dnaemia cutoff for preemptive therapy of cytomegalovirus infection in adult hematopoietic stem cell transplant recipients. | a randomized trial comparing a dnaemia cutoff of 10 000 copies per ml whole blood and first pp65 antigenemia positivity for initiation of preemptive therapy of human cytomegalovirus (hcmv) infection in adult hematopoietic stem cell transplant recipients was completed. dnaemia was chosen for cutoff definition since it is more automatable and standardizable than antigenemia, and more closely reflects the actual viral replication. the primary end point of the study was to compare the number of pati ... | 2008 | 18209721 |
| monitoring human cytomegalovirus infection in transplant recipients. | human cytomegalovirus (hcmv) infection remains one of the most challenging infectious complications in both solid organ transplant (sot) and hemopoietic stem cell transplant (hsct) recipients. in the last two decades advances have been made in the diagnosis and monitoring of hcmv infection in sot and hsct recipients following introduction of quantitative assays such as rapid virus isolation in blood (viremia), quantitation of pp65 in peripheral blood leukocytes (antigenemia), and quantitation of ... | 2008 | 18203657 |
| murine gammaherpesvirus 68 serum antibodies in general human population. | previous studies using elisa and virus neutralization test (vnt) have proved the presence of murine gammaherpesvirus 68 (mhv-68) serum antibodies in sera of laboratory staff working with mhv-68, as well as in the general population. in this study, we investigated the incidence of serum antibodies to mhv-68 and to human herpesviruses presumably antigenically similar to mhv-68, as herpes simplex virus 1 (hsv-1), human cytomegalovirus (hcmv), and epstein-barr virus (ebv), in general population usin ... | 2007 | 18197737 |
| dissecting the structural determinants of the interaction between the human cytomegalovirus ul18 protein and the cd85j immune receptor. | ul18 is a glycoprotein encoded by the human cytomegalovirus genome and is thought to play a pivotal role during human cytomegalovirus infection, although its exact function is still a matter of debate. ul18 shares structural similarity with mhc class i and binds the receptor cd85j on immune cells. besides ul18, cd85j binds mhc class i molecules. the binding properties of cd85j to mhc class i molecules have been thoroughly studied. conversely, very little information is available on the cd85j/ul1 ... | 2008 | 18178836 |
| evaluation of two set of primers for detection of immediate early gene ul123 of human cytomegalovirus (hcmv). | human cytomegalovirus (hcmv) is a widespread agent causing a life-long persistent and generally asymptomatic infection in immunocompetent individuals. in contrast, immunocompromised subjects are the most susceptible group to experience hcmv disease. first genes to be expressed during the replication cycle are the immediate early (ie) genes, the products of which have pleiotropic effects on host cell metabolism. aim of this study was to compare two set of primers in the optimization and standardi ... | 2008 | 18095191 |
| [polymorphism of human cytomegalovirus ul134 gene in low-passage clinical isolates]. | human cytomegalovirus (hcmv) displays genetic polymorphisms. nineteen open reading frames (orfs, ul133-ul151) found in the toledo strain of hcmv and other low-passage clinical isolates may be essential for viral infection. this study aimed to analyze the polymorphism of hcmv ul134 gene in clinical isolates and explore the relationship between the polymorphism and hcmv infection. | 2007 | 18082047 |
| the essential human cytomegalovirus gene ul52 is required for cleavage-packaging of the viral genome. | replication of human cytomegalovirus (hcmv) produces large dna concatemers of head-to-tail-linked viral genomes that upon packaging into capsids are cut into unit-length genomes. the mechanisms underlying cleavage-packaging and the subsequent steps prior to nuclear egress of dna-filled capsids are incompletely understood. the hitherto uncharacterized product of the essential hcmv ul52 gene was proposed to participate in these processes. to investigate the function of pul52, we constructed a delt ... | 2008 | 18077717 |
| modulation of natural killer cells by human cytomegalovirus. | human cytomegalovirus (hcmv) causes lifelong, persistent infections and its survival is under intense, continuous selective pressure from the immune system. a key aspect of hcmv's capacity for survival lies in immune avoidance. in this context, cells undergoing productive infection exhibit remarkable resistance to natural killer (nk) cell-mediated cytolysis in vitro. to date, six genes encoding proteins (ul16, ul18, ul40, ul83, ul141 and ul142) and one encoding a microrna (mir-ul112) have been i ... | 2008 | 18069056 |
| protein coding content of the ul)b' region of wild-type rhesus cytomegalovirus. | a recent comparison of two rhesus cytomegalovirus (rhcmv) genomes revealed that the region at the right end of the u(l) genome component (u(l)b') undergoes genetic alterations similar to those observed in serially passaged human cytomegalovirus (hcmv). to determine the coding content of authentic wild-type rhcmv in this region, the u(l)b' sequence was amplified from virus obtained from naturally infected rhesus macaques without passage in vitro. a total of 24 open reading frames (orfs) potential ... | 2008 | 18068749 |
| a fingerprint left by cytomegalovirus infection in the human t cell compartment. | latent infection with human cytomegalovirus (hcmv) is accompanied by a strong increase in the number of resting, effector-type cd4+ and cd8+ t cells with constitutive cytolytic activity in the circulation. longitudinal studies in kidney transplant recipients revealed that effector cells emerge early after the initial viral burst and acquire their stable phenotype in the months following primary infection. although it is yet unsettled whether these cells are all specific for cmv encoded or induce ... | 2008 | 18061537 |
| impairment of cytomegalovirus-specific cellular immune response as a risk factor for cytomegalovirus disease in transplant recipients. | human cytomegalovirus (cmv) infection is common in most people but nearly asymptomatic in immunocompetent individuals. after primary infection the virus persists throughout life in a latent form in a variety of tissues, particularly in precursor cells of the monocytic lineage. cmv reinfection and occurrence of disease are associated with immunosuppressive conditions. solid organ and bone marrow transplant patients are at high risk for cmv disease as they undergo immunosuppression. antiviral trea ... | 2008 | 18060322 |
| is combined pretransplantation seropositivity of kidney transplant recipients for cytomegalovirus antigens (pp150 and pp28) a predictor for protection against infection? | this study was aimed at detecting antibodies to the antigens which may contribute to protection against cytomegalovirus (cmv) infection after organ transplantation. | 2008 | 18059104 |
| differential inhibition of human cytomegalovirus (hcmv) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue. | human cytomegalovirus (hcmv) can be acquired sexually and is shed from the genital tract. cross-sectional studies in women show that changes in genital tract microbial flora affect hcmv infection and/or shedding. since genital microbial flora may affect hcmv infection or replication by stimulating cells through toll-like receptors (tlr), we assessed the effects of defined tlr-ligands on hcmv replication in foreskin fibroblasts and ectocervical tissue. poly i:c (a tlr3-ligand) and lipopolysacchar ... | 2007 | 18053251 |
| subgingival human cytomegalovirus correlates with increased clinical periodontal parameters and bacterial coinfection in periodontitis. | viruses from the herpesviridae family may be implicated in the pathogenesis of periodontal disease. the aim of this investigation was to compare the subgingival frequency of human cytomegalovirus (hcmv) in subjects affected by periodontitis to periodontally healthy subjects and to assess the correlation of hcmv with periodontal clinical parameters and periodontopathic bacteria. | 2007 | 18052702 |
| infection of human endothelium in vitro by cytomegalovirus causes enhanced expression of purinergic receptors: a potential virus escape mechanism? | human cytomegalovirus (cmv) uses different strategies to escape from human host defense reactions. previously we have observed that infection of endothelial cells with cmv in vitro leads to enhanced activity of endothelial ectonucleotidases. these ectoenzymes are responsible for hydrolysis of extracellular adenine nucleotides, resulting in the formation of adenosine. infection with cmv in vivo therefore may result in local increase of adenosine production, providing an anti-inflammatory and anti ... | 2007 | 18049120 |
| immunoassay with cytomegalovirus early antigens from gene products p52 and cm2 (ul44 and ul57) detects active infection in patients with chronic fatigue syndrome. | to investigate whether the use of recombinant early antigens for detection of antibodies to human cytomegalovirus (hcmv) gene products cm(2) (ul44, ul57) and p52 (ul44) is specific in the diagnosis and differentiation of active hcmv infection in a subset of patients with chronic fatigue syndrome (cfs), a diagnosis which is often missed by the current elisa assay that uses crude viral lysate antigen. | 2008 | 18037660 |
| human cytomegalovirus dna replication: antiviral targets and drugs. | human cytomegalovirus (hcmv) infection is associated with severe morbidity and mortality in immunocompromised individuals, in particular transplant recipients and aids patients, and is the most frequent congenital viral infection in humans. there are currently five drugs approved for hcmv treatment: ganciclovir and its prodrug valganciclovir, foscarnet, cidofovir and fomivirsen. these drugs have provided a major advance in hcmv disease management, but they suffer from poor bioavailability, signi ... | 2008 | 18027349 |
| comments on "cytomegalovirus (cmv)-encoded ul144 (truncated tumor necrosis factor receptor) and outcome of congenital cmv infection". | 2007 | 18008260 | |
| valproic acid interferes with antiviral treatment in human cytomegalovirus-infected endothelial cells. | the endothelium represents a natural site of human cytomegalovirus (hcmv) infection involved in viral spreading and persistence. moreover, hcmv infection of endothelial cells has been associated with different pathological conditions of the cardiovascular system. here, the influence of the antiepileptic drug valproic acid (vpa) was investigated on hcmv replication in human umbilical vein endothelial cells alone or in combination with the antiviral drugs ganciclovir, cidofovir or foscarnet. | 2008 | 18006438 |
| [capture and detection of human cytomegalovirus (hcmv) igm by a recombinant multi-epitope chimeric antigen]. | to establish a sensitive and specific antibody-capture enzyme-linked immunosorbent assay (ac-elisa) method to detect serum igm against human cytomegalovirus (hcmv) by expressing a recombinant hcmv multi-epitope cheimeric antigen through genetic engineering. the dominant epitopes of hcmv were analyzed and selected by computer software a recombinant multi-epitope chimeric antigen expression vector including hcmv dna was constructed, and transformed into e. coli bl21(de3). the antigen was abundantl ... | 2007 | 17989044 |
| cd4+ t cells specific for glycoprotein b from cytomegalovirus exhibit extreme conservation of t-cell receptor usage between different individuals. | antigen-specific cd8(+) cytotoxic t cells often demonstrate extreme conservation of t-cell receptor (tcr) usage between different individuals, but similar characteristics have not been documented for cd4(+) t cells. cd4(+) t cells predominantly have a helper immune role, but a cytotoxic cd4(+) t-cell subset has been characterized, and we have studied the cytotoxic cd4(+) t-cell response to a peptide from human cytomegalovirus glycoprotein b presented through hla-drb*0701. we show that this pepti ... | 2008 | 17986665 |
| high prevalence of an active cytomegalovirus infection in the appendix of immunocompetent patients with acute appendicitis. | appendicitis is a very common surgical diagnosis with unclear pathology. human cytomegalovirus (hcmv) can modulate our immune system and has been associated with inflammatory bowel disease (ibd) and various other inflammatory diseases. | 2008 | 17973298 |
| the infection of human primary cells and cell lines by human cytomegalovirus: new tropism and new reservoirs for hcmv. | human cytomegalovirus (hcmv) infection is asymptomatic in common persons and could reactive in immunosuppression groups. hcmv was considered as endothelial cells (ec) tropism and leukocyte tropism. we hypothesized that hcmv will infect other cell types from human which have not been reported yet. the hcmv released from human mrc-5 was inoculated into eight human primary cells and cell lines, including human dermal fibroblasts (hdf), human embryo-chondrocytes (hec), human embryo-myoblasts (hem), ... | 2008 | 17963941 |
| evaluation of the presence of selected viral and bacterial nucleic acids in pericardial samples from dogs with or without idiopathic pericardial effusion. | many viruses have been identified in pericardial fluid and in tissue samples from humans with pericarditis by means of molecular diagnostics. in canine idiopathic pericardial effusion there is as yet no conclusive evidence to support the involvement of an infectious agent. this study was designed to investigate a possible relationship between idiopathic pericardial effusion in dogs and viruses most commonly encountered in humans affected with viral pericarditis. coxsackievirus b3 rna, influenza ... | 2009 | 17959400 |
| human cytomegalovirus ul145 gene is highly conserved among clinical strains. | human cytomegalovirus (hcmv), a ubiquitous human pathogen, is the leading cause of birth defects in newborns. a region (referred to as ul/b') present in the toledo strain of hcmv and low-passage clinical isolates) contains 22 additional genes,which are absent in the highly passaged laboratory strain ad169. one of these genes,ul145 open reading frame (orf), is located between the highly variable genes ul144 and ul146. to assess the structure of the ul145 gene,the ul145 orf was amplified by pcr an ... | 2007 | 17954963 |
| knockdown of hdaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression. | the cellular protein human daxx (hdaxx), a component of nuclear domain 10 structures, is known to mediate transcriptional repression of human cytomegalovirus immediate-early (ie) gene expression upon infection of permissive cell types, at least in part, by regulation of chromatin structure around the major ie promoter (miep). as it is now clear that differentiation-dependent regulation of the miep also plays a pivotal role in the control of latency and reactivation, we asked whether hdaxx-mediat ... | 2007 | 17947514 |
| characterization of the human cytomegalovirus gh/gl/ul128-131 complex that mediates entry into epithelial and endothelial cells. | the entry of human cytomegalovirus (hcmv) into biologically relevant epithelial and endothelial cells involves endocytosis followed by low-ph-dependent fusion. this entry pathway is facilitated by the hcmv ul128, ul130, and ul131 proteins, which form one or more complexes with the virion envelope glycoprotein gh/gl. gh/gl/ul128-131 complexes appear to be distinct from the gh/gl/go complex, which likely facilitates entry into fibroblasts. in order to better understand the assembly and protein-pro ... | 2008 | 17942555 |
| accumulation of substrates of the anaphase-promoting complex (apc) during human cytomegalovirus infection is associated with the phosphorylation of cdh1 and the dissociation and relocalization of apc subunits. | cell cycle dysregulation upon human cytomegalovirus (hcmv) infection of human fibroblasts is associated with the inactivation of the anaphase-promoting complex (apc), a multisubunit e3 ubiquitin ligase, and accumulation of its substrates. here, we have further elucidated the mechanism(s) by which hcmv-induced inactivation of the apc occurs. our results show that cdh1 accumulates in a phosphorylated form that may prevent its association with and activation of the apc. the accumulation of cdh1, bu ... | 2008 | 17942546 |
| inhibition of the cyclin-dependent kinases at the beginning of human cytomegalovirus infection specifically alters the levels and localization of the rna polymerase ii carboxyl-terminal domain kinases cdk9 and cdk7 at the viral transcriptosome. | we previously reported that defined components of the host transcription machinery are recruited to human cytomegalovirus immediate-early (ie) transcription sites, including cdk9 and cdk7 (s. tamrakar, a. j. kapasi, and d. h. spector, j. virol. 79:15477-15493, 2005). in this report, we further document the complexity of this site, referred to as the transcriptosome, through identification of additional resident proteins, including viral ul69 and cellular cyclin t1, brd4, histone deacetylase 1 (h ... | 2008 | 17942543 |
| expression of a human cytomegalovirus latency-associated homolog of interleukin-10 during the productive phase of infection. | the human cytomegalovirus ul111a region is active during both productive and latent phases of infection. during productive infection, the virus expresses orf79, a protein with oncogenic properties, and cmvil-10, a functional homolog of human il-10. during latent infection of myeloid progenitor cells, an alternately spliced variant of cmvil-10, termed latency-associated (la) cmvil-10 has previously been identified. to determine whether lacmvil-10 transcription occurs during productive infection, ... | 2008 | 17942134 |
| regulated nucleocytoplasmic trafficking of viral gene products: a therapeutic target? | the study of viral proteins and host cell factors that interact with them has represented an invaluable contribution to understanding of the physiology as well as associated pathology of key eukaryotic cell processes such as cell cycle regulation, signal transduction and transformation. similarly, knowledge of nucleocytoplasmic transport is based largely on pioneering studies performed on viral proteins that enabled the first sequences responsible for the facilitated transport through the nuclea ... | 2008 | 17933596 |
| monitoring of human cytomegalovirus infection in immunosuppressed patients using real-time pcr on whole blood. | quantitative monitoring of human cytomegalovirus (hcmv) is currently used in the follow-up of immunosuppressed patients. | 2007 | 17904901 |
| accurate identification of paraprotein antigen targets by epitope reconstruction. | we describe the first successful clinical application of a new discovery technology, epitope-mediated antigen prediction (e-map), to the investigation of multiple myeloma. until now, there has been no reliable, systematic method to identify the cognate antigens of paraproteins. e-map is a variation of previous efforts to reconstruct the epitopes of paraproteins, with the significant difference that it provides enough epitope sequence data so as to enable successful protein database searches. we ... | 2008 | 17878398 |
| differential effects of prednisolone and azathioprine on the development of human cytomegalovirus replication post liver transplantation. | we sought to investigate the impact of different immunosuppressive regimens on human cytomegalovirus (hcmv) incidence and replication dynamics in a cohort of 256 patients after liver transplantation. | 2007 | 17876273 |
| the varicella-zoster virus dna encapsidation genes: identification and characterization of the putative terminase subunits. | the putative dna encapsidation genes encoded by open reading frames (orfs) 25, 26, 30, 34, 43, 45/42 and 54 were cloned from varicella-zoster virus (vzv) strain ellen. sequencing revealed that the ellen orfs were highly conserved at the amino acid level when compared to those of 19 previously published vzv isolates. additionally, rt-pcr provided the first evidence that orf45/42 was expressed as a spliced transcript in vzv-infected cells. all seven orfs were expressed in vitro and full length pro ... | 2007 | 17868947 |
| human cytomegalovirus is present in odontogenic cysts. | recent studies suggest that some viruses, including human cytomegalovirus (cmv), may be involved in the pathogenesis of periapical lesions. since periapical cysts (pcs) represent the next stage in the evolution of periapical granuloma, it seemed reasonable to investigate the presence of cmv in pcs and any possible relationship between its presence and the clinical features of those cysts, as well as to compare the results obtained with corresponding findings in non-inflammatory lesions, like odo ... | 2007 | 17803633 |
| acceleration of allograft failure by cytomegalovirus. | a number of human herpesviruses are important opportunistic pathogens that have been associated with increased morbidity and mortality in transplant recipients including human cytomegalovirus (hcmv), hhv6, hhv7, hhv8 as well as hsv-1, vzv. however, hcmv has been linked both epidemiologically and through the use of animal models to the acceleration of acute and chronic allograft rejection. this review will cover the pathophysiology, epidemiology, and mechanisms of cmv-associated disease in the se ... | 2007 | 17716883 |
| serum proteomics with seldi-tof-ms in congenital human cytomegalovirus hepatitis. | human cytomegalovirus (hcmv) is a widespread pathogen, the most common congenital viral infection, and the leading cause of infant hepatitis syndrome. in this study, serum samples were collected from 20 hcmv-infected infants with hepatitis and 25 controls. of the 25 infants in the control group, 5 were infected with hcmv but without hepatitis, 10 had hepatitis but no hcmv infection, and 10 were healthy. proteomic expression in the serum was detected by wcx2 chips and surface-enhanced laser desor ... | 2007 | 17705191 |
| cytomegalovirus ul131-128 products promote gb conformational transition and gb-gh interaction during entry into endothelial cells. | herpesviruses use gb and gh-gl glycoproteins to execute fusion. other virus-specific glycoproteins are required for receptor binding and fusion activation. the human cytomegalovirus (hcmv) ul131-128 proteins are essential for the infection of leukocytes, endothelial cells (ecs), and many epithelial cell lines. here we show that ul131-128 play a role in a chain of events involving gb and gh during hcmv entry into ecs. an hcmv strain bearing the wild-type (wt) ul131-128 locus exhibited a gb transi ... | 2007 | 17686875 |
| antisense transcription in the human cytomegalovirus transcriptome. | human cytomegalovirus (hcmv) infections are prevalent in human populations and can cause serious diseases, especially in those with compromised or immature immune systems. the hcmv genome of 230 kb is among the largest of the herpesvirus genomes. although the entire sequence of the laboratory-adapted ad169 strain of hcmv has been available for 18 years, the precise number of viral genes is still in question. we undertook an analysis of the hcmv transcriptome as an approach to enumerate and analy ... | 2007 | 17686857 |
| the s phase of the cell cycle and its perturbation by human cytomegalovirus. | human cytomegalovirus (hcmv) is a complex human herpesvirus that is known to productively infect a wide range of cell types. in addition, it has been suggested to contribute to some proliferative disorders, particularly atherosclerosis. consistent with this, a number of studies have shown that hcmv profoundly affects normal cell cycle control. specifically, the virus can stimulate early entry into s phase thus ensuring adequate resources for viral dna replication. importantly, however, the virus ... | 2007 | 17676653 |
| usefulness of interleukin-10 detection in lung transplant patients with human cytomegalovirus infection with respect to virus persistence. | human cytomegalovirus (hcmv) infection in lung transplant patients induces an inflammatory response, including local production of cytokines involved in viral clearance. the aim of this study was to evaluate the potential value of monitoring interleukin (il)-10 with respect to hcmv persistence in blood and/or bronchoalveolar lavage (bal). | 2007 | 17667821 |
| a model of human cytomegalovirus infection in severe combined immunodeficient mice. | animal models for the evaluation of therapies against human cytomegalovirus (hcmv) are limited due to the species-specific replication of cmv. models utilizing human fetal tissues implanted into scid mice have been used but tend to be labor intensive and require human tissues. we therefore developed a model using hcmv-infected human foreskin fibroblasts (hff) seeded onto a biodegradable gelatin matrix (gelfoam). infected hffs are then implanted subcutaneously into scid mice. we next evaluated tw ... | 2007 | 17658624 |
| dislocation of an endoplasmic reticulum membrane glycoprotein involves the formation of partially dislocated ubiquitinated polypeptides. | accumulation of improperly folded polypeptides in the endoplasmic reticulum (er) can trigger a stress response that leads to the export of aberrant proteins into the cytosol and their ultimate proteasomal degradation. human cytomegalovirus encodes a type i glycoprotein, us11, that binds to nascent mhc class i heavy chain molecules and causes their dislocation from the er to the cytosol where they are degraded by the proteasome. examination of us11-mediated class i degradation has identified a ho ... | 2007 | 17650499 |
| host immune system gene targeting by a viral mirna. | virally encoded micrornas (mirnas) have recently been discovered in herpesviruses. however, their biological roles are mostly unknown. we developed an algorithm for the prediction of mirna targets and applied it to human cytomegalovirus mirnas, resulting in the identification of the major histocompatibility complex class i-related chain b (micb) gene as a top candidate target of hcmv-mir-ul112. micb is a stress-induced ligand of the natural killer (nk) cell activating receptor nkg2d and is criti ... | 2007 | 17641203 |
| an importin alpha/beta-recognized bipartite nuclear localization signal mediates targeting of the human herpes simplex virus type 1 dna polymerase catalytic subunit pul30 to the nucleus. | although the 1235 amino acids human herpes simplex virus type 1 (hsv-1) dna polymerase catalytic subunit, pul30, is essential for hsv-1 replication in the nucleus of host cells, little information is available regarding its nuclear import mechanism. the present study addresses this issue directly, characterizing pul30's nuclear import pathway for the first time using quantitative confocal laser scanning microscopy (clsm) on living cells, and fluorescent binding assays. in addition to a previousl ... | 2007 | 17640102 |
| recruitment of human cytomegalovirus immediate-early 2 protein onto parental viral genomes in association with nd10 in live-infected cells. | the human cytomegalovirus (hcmv) immediate-early 2 (ie2) transactivator has previously been shown to form intranuclear, dot-like accumulations in association with subnuclear structures known as promyelocytic leukemia protein (pml) nuclear bodies or nd10. we recently observed that ie2 can form dot-like structures even after infection of pml knockdown cells, which lack genuine nd10. to further analyze the determinants of ie2 subnuclear localization, a recombinant hcmv expressing ie2 fused to the e ... | 2007 | 17626080 |
| human cytomegalovirus glycoprotein b genotypes in brazilian mothers and their congenitally infected infants. | a case-control study design was used in order to compare the distribution of human cytomegalovirus (hcmv) glycoprotein b (gb) genotypes in 48 mothers of 49 congenitally infected infants with that observed in 144 mothers of 146 uninfected infants to study genetic variation of hcmv strains and maternal-fetal transmission. congenital infection with hcmv was characterized by dna detection and virus isolation from two urine or saliva samples collected prior to the third week of life. genotyping of hc ... | 2007 | 17597488 |
| human cytomegalovirus gene expression is silenced by daxx-mediated intrinsic immune defense in model latent infections established in vitro. | in addition to productive lytic infections, herpesviruses such as human cytomegalovirus (hcmv) establish a reservoir of latently infected cells that permit lifelong colonization of the host. when latency is established, the viral immediate-early (ie) genes that initiate the lytic replication cycle are not expressed. hcmv ie gene expression at the start of a lytic infection is facilitated by the viral pp71 protein, which is delivered to cells by infectious viral particles. pp71 neutralizes the da ... | 2007 | 17596307 |
| comparison of two methods used for monitoring low-copy cytomegalovirus infection in a patient with chronic myeloid leukemia after unrelated umbilical cord blood transplantation. | detection of human cytomegalovirus (cmv, hhv-5) dna in clinical specimens is considered a cornerstone in the diagnosis of hhv-5 disease. the present study compared two quantitative methods used for diagnosing cytomegalovirus infection in a 21-year-old woman with chronic myeloid leukemia after an unrelated umbilical cord blood transplantation. | 2007 | 17557148 |
| vaccine strategies against human cytomegalovirus infection. | human cytomegalovirus (hcmv) disease is a major cause of morbidity and mortality in neonates and immunocompromised populations, such as transplant recipients and hiv-infected patients. the development of a vaccine to prevent hcmv infection or disease has been assigned the highest priority by the us institute of medicine. although, after 30 years of intensive study, a clinically licensed vaccine is still not available, significant progress has been made in the field of hcmv vaccine development, a ... | 2007 | 17547509 |
| [correlation between mhc class i-related chain a gene *008 allele and human cytomegalovirus infection]. | to investigate the correlation between mhc class i-related chain a (mica) gene *008 allele and human cytomegalovirus (hcmv) infection. | 2007 | 17545046 |
| endothelial cells' activation and apoptosis induced by a subset of antibodies against human cytomegalovirus: relevance to the pathogenesis of atherosclerosis. | human cytomegalovirus (hcmv) is involved in the pathogenesis of atherosclerosis. we have previously shown in patients with atherosclerosis that antibodies directed against the hcmv-derived proteins us28 and ul122 are able to induce endothelial cell damage and apoptosis of non-stressed endothelial cells through cross-rection with normally expressed surface molecules. our aim was to dissect the molecular basis of such interaction and to investigate mechanisms linking innate immunity to atheroscler ... | 2007 | 17534423 |
| cytomegalovirus destruction of focal adhesions revealed in a high-throughput western blot analysis of cellular protein expression. | human cytomegalovirus (hcmv) systematically manages the expression of cellular functions, rather than exerting the global shutoff of host cell protein synthesis commonly observed with other herpesviruses during the lytic cycle. while microarray technology has provided remarkable insights into viral control of the cellular transcriptome, hcmv is known to encode multiple mechanisms for posttranscriptional and post-translation regulation of cellular gene expression. high-throughput western blotting ... | 2007 | 17522202 |
| herpesvirus latency confers symbiotic protection from bacterial infection. | all humans become infected with multiple herpesviruses during childhood. after clearance of acute infection, herpesviruses enter a dormant state known as latency. latency persists for the life of the host and is presumed to be parasitic, as it leaves the individual at risk for subsequent viral reactivation and disease. here we show that herpesvirus latency also confers a surprising benefit to the host. mice latently infected with either murine gammaherpesvirus 68 or murine cytomegalovirus, which ... | 2007 | 17507983 |
| a prospective assessment of cytomegalovirus immune evasion gene transcription profiles in transplant patients with cytomegalovirus infection. | the cytomegalovirus (cmv) immune evasion genes us3, us6, and us11 may disrupt the host immune response via downregulation of major histocompatibility complex molecules. transplant recipients with cmv infection were prospectively assessed for immune evasion gene expression. | 2007 | 17496536 |
| synthesis and biological evaluation of some alpha-[6-(1'-carbamoylalkylthio)-1 h-pyrazolo[3,4-d]pyrimidin-4-yl]thioalkylcarboxamide acyclonucleosides. | the reaction of 1h-pyrazolo[3,4-d]pyrimidin-4,6-dithione 11 with compounds 12a-c produces ethyl alpha-[6-(1'-carboethoxyalkylthio)-1 h-pyrazolo[3,4-d]pyrimidin-4-yl]thioalkylates 13a-c, respectively. these heterocycles were alkylated, separately, with alkylating agents 14, 15, and 16 to afford, predominately, the n(1)-acyclic nucleosides (17-19)a-c, which were deprotected to give the desired products (20-22)a-c. all synthetic compounds were characterized on the basis of their physical and spectr ... | 2007 | 17479430 |
| exploiting the yeast l-a viral capsid for the in vivo assembly of chimeric vlps as platform in vaccine development and foreign protein expression. | a novel expression system based on engineered variants of the yeast (saccharomyces cerevisiae) dsrna virus l-a was developed allowing the in vivo assembly of chimeric virus-like particles (vlps) as a unique platform for a wide range of applications. we show that polypeptides fused to the viral capsid protein gag self-assemble into isometric vlp chimeras carrying their cargo inside the capsid, thereby not only effectively preventing proteolytic degradation in the host cell cytosol, but also allow ... | 2007 | 17476337 |
| [mechanism of drug-resistance in human cytomegalovirus]. | 2007 | 17455666 | |
| [human cytomegalovirus infection]. | 2007 | 17455606 | |
| functional role of mglur1 and mglur4 in pilocarpine-induced temporal lobe epilepsy. | altered expression and distribution of neurotransmitter receptors, including metabotropic glutamate receptors (mglurs), constitute key aspects in epileptogenesis, impaired hippocampal excitability and neuronal degeneration. mglur1 mediates predominantly excitatory effects, whereas mglur4 acts as inhibitory presynaptic receptor. increased hippocampal expression of mglur1 and mglur4 has been observed in human temporal lobe epilepsy (tle). in this study, we address whether genetic mglur1 upregulati ... | 2007 | 17446080 |
| pathogenesis and antibody response to a cytomegalovirus infection in newborn rats. | the present study described the kinetics of rat cytomegalovirus (rcmv) infection in newborn rats by monitoring infectious virus and viral antigens in various organs, viral dna in the blood (dnaemia) and antibody response. these parameters were evaluated quantitatively using double-antibody sandwich elisa (das-elisa), real-time pcr, indirect elisa and virus infectivity assay. for the first time das-elisa was used for detection of rcmv antigen directly from organ samples. the relationships between ... | 2007 | 17432941 |
| an intrastrain variation of a 189-bp repeat region in the human cytomegalovirus replication origin. | a 189-bp repeat has been reported previously to be present in the replication origin (ori) of human cytomegalovirus (hcmv) towne strain and to contain essential and supporting sequences (chen et al., arch. virol. 141, 13301 (1996)). in this study, clones of hcmv strain towne were isolated and analyzed for the copy number of the 189-bp repeat within its ori (orilyt). southern blot analysis revealed that out of 14 clones, two contained a dimer, two contained a tetramer, and the remaining contained ... | 2007 | 17432940 |
| the late promoter of the human cytomegalovirus viral dna polymerase processivity factor has an impact on delayed early and late viral gene products but not on viral dna synthesis. | transcription of the dna polymerase processivity factor gene (ul44) of human cytomegalovirus initiates at three distinct start sites, which are differentially regulated during productive infection. two of these start sites, the distal and proximal sites, are active at early times, and the middle start site is active at only late times after infection (f. leach and e. s. mocarski, j. virol. 63:1783-1791, 1989). compared to the wild type, ul44 gene expression was lower for recombinant viruses with ... | 2007 | 17409154 |
| expression of gdnf transgene in astrocytes improves cognitive deficits in aged rats. | glial cell line-derived neurotrophic factor (gdnf) was assayed for its neurotrophic effects against the neuronal atrophy that causes cognitive deficits in old age. aged fisher 344 rats with impairment in the morris water maze received intrahippocampal injections at the dorsal ca1 area of either a lentiviral vector encoding human gdnf or the same vector encoding human green fluorescent protein as a control. recombinant lentiviral vectors constructed with human cytomegalovirus promotor and pseudot ... | 2008 | 17399854 |
| cytoplasmic envelopment of human cytomegalovirus requires the postlocalization function of tegument protein pp28 within the assembly compartment. | the assembly of herpesvirus remains incompletely defined due to the structural complexity of these viruses. although the assembly of the capsid of these large dna viruses is well studied and reasonably well conserved for all members of this diverse family of viruses, the cytoplasmic processes of tegumentation and envelopment are not well understood. the virion of the largest human herpesvirus, human cytomegalovirus (hcmv), contains over 70 virus-encoded proteins that are incorporated during a nu ... | 2007 | 17392372 |
| the human cytomegalovirus mhc class i homolog ul18 inhibits lir-1+ but activates lir-1- nk cells. | the inhibitory leukocyte ig-like receptor 1 (lir-1, also known as ilt2, cd85j, or lilrb1) was identified by its high affinity for the human cmv (hcmv) mhc class i homolog gpul18. the role of this lir-1-gpul18 interaction in modulating nk recognition during hcmv infection has previously not been clearly defined. in this study, lir-1(+) nkl cell-mediated cytotoxicity was shown to be inhibited by transduction of targets with a replication-deficient adenovirus vector encoding ul18 (rad-ul18). fibrob ... | 2007 | 17372005 |
| synthesis and antiviral evaluation of alkoxyalkyl-phosphate conjugates of cidofovir and adefovir. | esterification of cidofovir (cdv), an antiviral nucleoside phosphonate, with alkyl or alkoxyalkyl groups increases antiviral activity by enhancing cell uptake and conversion to cdv diphosphate. hexadecyloxypropyl-cdv (hdp-cdv) has been shown to be 40-100 times more active than cdv in vitro in cells infected with herpes group viruses, variola, cowpox, vaccinia or ectromelia viruses. since the first phosphorylation of cdv may be rate limiting, we synthesized the hexadecyloxypropyl-phosphate (hdp-p ... | 2007 | 17367874 |
| effects of jinye baidu granule on fetal growth and development with maternal active human cytomegalovirus infection. | to evaluate the effects of jinye baidu granule ( jybdg), a traditional chinese medicine compound prescription, on fetal growth and development with maternal active human cytomegalovirus infection. | 2006 | 17361519 |
| human cytomegalovirus glycoprotein b genotype correlates with different symptoms of infected infants. | human cytomegalovirus (hcmv) is the pathogen of cytomegalic inclusion disease of infants. hcmv strains can be classified into four genotypes of glycoprotein b (gb). there are limited data concerning links between clinical symptoms and hcmv genotypes. the aims of the present study were to determine the genotype of hcmv isolates from pediatric patients who have different symptoms on the assumption that the gb genotype may influence the outcomes of congenital and prenatal hcmv infection. | 2007 | 17356299 |
| development of human cytomegalovirus-specific t cell immunity during primary infection of pregnant women and its correlation with virus transmission to the fetus. | we sought to study the development of human cytomegalovirus (hcmv)-specific t cell-mediated immune responses during primary hcmv infection in pregnancy. | 2007 | 17330798 |
| extensive sequence variation exists among isolates of murine cytomegalovirus within members of the m02 family of genes. | murine cytomegalovirus (mcmv) is a widely used model for human cytomegalovirus (hcmv) and has facilitated many important discoveries about the biology of cmvs. most of these studies are conducted using the laboratory mcmv strains smith and k181. however, wild-derived isolates of mcmv, like hcmv clinical isolates, exhibit genetic variation from laboratory strains, particularly at the ends of their genomes in areas containing known or putative immune-evasion and tropism genes. this study analysed ... | 2007 | 17325348 |
| dna vaccines expressing glycoprotein complex ii antigens gm and gn elicited neutralizing antibodies against multiple human cytomegalovirus (hcmv) isolates. | human cytomegalovirus (hcmv) glycoprotein complex ii (gcii) consists of two glycoproteins, gm and gn. although gcii specific igg purified from hcmv positive patient sera can neutralize hcmv, there has been no report describing the generation of virus-neutralizing antibodies by immunization with individual recombinant gm or gn antigens. in the current study, gm and gn antigens were expressed by the mammalian expression vector pjw4303 and used as dna vaccines to determine the immunogenicity of the ... | 2007 | 17287056 |
| multimerization of human cytomegalovirus regulatory protein ul69 via a domain that is conserved within its herpesvirus homologues. | the ul69 protein of human cytomegalovirus is a multifunctional regulatory protein that has counterparts in all herpesviruses. some of these proteins have been shown to function primarily at the post-transcriptional level in promoting nuclear export of viral transcripts. consistently, this group has reported recently that pul69 is an rna-binding, nucleocytoplasmic shuttling protein that facilitates the cytoplasmic accumulation of unspliced mrna via its interaction with the cellular mrna export fa ... | 2007 | 17251556 |
| human cytomegalovirus enhances a. actinomycetemcomitans adherence to cells. | adherence of actinobacillus actinomycetemcomitans to epithelial cells is an important step in periodontal disease pathogenesis. recent publications describe the subgingival presence of a wide array of viruses [e.g., human cytomegalo-virus (hcmv)]. since viruses can increase cellular susceptibility for bacterial adherence, we investigated whether hcmv renders epithelial cells more prone to adherence by actinobacillus actinomycetemcomitans. cultivated hela and primary epithelial cells were shown t ... | 2007 | 17251519 |
| antiviral activity of berberine and related compounds against human cytomegalovirus. | berberine chloride (1) and the structurally related compounds were assessed for the anti-human cytomegalovirus (hcmv) activity using the plaque assay. the anti-hcmv activity (ic(50) 0.68 microm) of 1 was equivalent to that (ic(50) 0.91 microm) of ganciclovir (gcv). the mechanism of action by which 1 inhibits the replication of hcmv is presumed to be different from that of gcv; 1 would interfere with intracellular events after virus penetration into the host cells and before viral dna synthesis. | 2007 | 17239594 |