Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| sulfated k5 escherichia coli polysaccharide derivatives: a novel class of candidate antiviral microbicides. | antiviral microbicides, topical agents that prevent sexually transmitted infections, mainly work by blocking the interaction between viral proteins and cell surface components. in many instances, virus-cell interaction is mediated by cell surface heparan sulfate proteoglycans (hspgs). hspgs are exploited as attachment receptors by three sexually transmitted viruses: human immunodeficiency virus (hiv), herpes simplex virus (hsv) and human papilloma virus (hpv). since these viruses can either infe ... | 2009 | 19447134 |
| what is your diagnosis? acute herpes simplex virus type 1 stomatitis. | 2009 | 19445307 | |
| synthesis, antiviral activity, and stability of nucleoside analogs containing tricyclic bases. | a series of 3,9-dihydro-9-oxo-5h-imidazo[1,2-a]purine nucleosides (tricylic nucleosides) were synthesized from 9-[4-alpha-(hydroxymethyl)cyclopent-2-ene-1-alpha-yl]guanine (cbv) 5, (-)-beta-d-(2r,4r)-1,3-dioxolane-guanosine (dxg) 6, 3'-azido-3'-deoxy-guanosine (azg) 7, and 2'-c-methylguanosine 8. their in vitro activity against hiv and hcv was evaluated and correlated to their ability to degrade to their purine counterpart. | 2009 | 19433343 |
| the herpes simplex virus-induced demise of keratinocytes is associated with a dysregulated pattern of p63 expression. | p63 plays a pivotal role in the development and maintenance of stratified epithelial tissues. in an effort to gain insight into the pathogenic mechanisms of skin infections caused by hsv-1 and hsv-2, we determined the patterns of p63 expression in primary keratinocytes and in the hacat cell line. the levels of deltanp63alpha and a 50kda p73 isoform were decreased, bax-alpha remained unaffected, while the expressions of the bax-beta, tap63gamma and a 44.5kda p73 isoform were highly increased in b ... | 2009 | 19427396 |
| 7-o-methylkaempferol and -quercetin glycosides from the whole plant of nervilia fordii. | five new 7-o-methylkaempferol and -quercetin glycosides, namely, nervilifordins a-e (1-5), were isolated from the whole plant of nervilia fordii, together with seven known flavonoids (6, 7, and 9-13) and one known coumarin (8). their structures were elucidated on the basis of extensive spectroscopic analyses, including hsqc, hmbc, roesy, and chemical methods. compounds 1-3 and 6-13 were evaluated for their anti-herpes simplex virus 1 (hsv-1) activity and cytotoxicity on african green monkey kidn ... | 2009 | 19422205 |
| comparison of the pseudorabies virus us9 protein with homologs from other veterinary and human alphaherpesviruses. | pseudorabies virus (prv) us9 is a small, tail-anchored (ta) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. we cloned the us9 homologs from two human pathogens, varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1), as well as two veterinary pathogens, equine herpesvirus type 1 (ehv-1) and bovine herpesvirus type 1 (bhv-1), and fused them to enhanced green fluorescent ... | 2009 | 19420087 |
| a case of herpetic epithelial keratitis after triamcinolone acetonide subtenon injection. | we report a case of herpes simplex virus (hsv) epithelial keratitis that developed after a subtenon triamcinolone acetonide (ta) injection. | 2009 | 19411969 |
| reconstitution of uracil dna glycosylase-initiated base excision repair in herpes simplex virus-1. | herpes simplex virus-1 is a large double-stranded dna virus that is self-sufficient in a number of genome transactions. hence, the virus encodes its own dna replication apparatus and is capable of mediating recombination reactions. we recently reported that the catalytic subunit of the hsv-1 dna polymerase (ul30) exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities that are integral to base excision repair. base excision repair is required to maintain genome stability as ... | 2009 | 19411250 |
| induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized echinacea, a potent antiviral herbal extract. | several viruses associated with upper respiratory diseases have been shown to stimulate the secretion of pro-inflammatory cytokines, including chemokines, sometimes in the absence of viral cytopathology. we evaluated the ability of a standardized preparation of the popular herbal medicine echinacea (echinaforce, an ethanol extract of herb and roots of e. purpurea, and containing known concentrations of marker compounds) to inhibit the viral induction of various cytokines in a line of human bronc ... | 2009 | 19409931 |
| synthesis and biological activity of amino acid esters of acyclovir. | 2009 | 19400144 | |
| reactivated herpetic keratitis following laser in situ keratomileusis. | a 40-year-old woman presented with an unusual form of corneal herpetic reactivation 5 weeks after laser in situ keratomileusis. the cornea showed diffuse edema and a stromal ring infiltrate (disciform in appearance). oral antiviral and topical steroids in tapered dosages were administered. the infiltrate disappeared within 6 weeks. progressive improvement was also seen in all the parameters on pentacam scans, including the pachymetry, keratometry readings, and elevation on the anterior float. he ... | 2009 | 19393899 |
| hsv-1 encephalitis complicated by cerebral hemorrhage in an hiv-positive person. | although herpes simplex virus type 1 (hsv-1) is the most common cause of sporadic encephalitis in immunocompetent adults, it is an unusual cause of encephalitis in patients with hiv/aids. we report the case of a 56-year-old man with recently diagnosed hiv infection who presented with subacute mental status changes, fever, and temporal lobe abnormalities evident on brain imaging. results of a polymerase chain reaction assay of the cerebrospinal fluid were positive for hsv-1. his course was compli ... | 2009 | 19388182 |
| pediatric herpes simplex virus keratitis. | to report the clinical characteristics and visual outcomes of pediatric herpes simplex virus (hsv) keratitis. | 2009 | 19387223 |
| [novel mechanism of herpes simplex virus type-i infection]. | 2009 | 19378786 | |
| titration of variant hsv1-tk gene expression to determine the sensitivity of 18f-fhbg pet imaging in a prostate tumor. | because of its high selectivity and specificity for the imaging reporter probe 9-(4-(18)f-fluoro-3-[hydroxymethyl]butyl)guanine ((18)f-fhbg), the herpes simplex virus type 1 thymidine kinase (hsv1-tk) variant sr39tk is actively being studied as a pet reporter gene. we recently demonstrated the capability of using a prostate-specific transcriptional amplification pet reporter vector, adtsta-sr39tk, to target prostate cancer lymph node metastasis. however, one area that warrants further study is t ... | 2009 | 19372484 |
| induction of sialyl-lex expression by herpes simplex virus type 1 is dependent on viral immediate early rna-activated transcription of host fucosyltransferase genes. | we have previously shown that varicella-zoster virus (vzv) and cytomegalovirus (cmv) infection of diploid human fibroblasts (hel) results in neo-expression of lewis antigens sialyl lewis x (sle(x)) and lewis y (le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltransferase genes (fut1, fut3, fut5, and fut6), whose gene products are responsible for the synthesis of le antigens. here, we show that herpes simplex virus type 1 (hsv-1) also induce ... | 2009 | 19369700 |
| immunomodulatory roles of topical dinitrochlorobenzene treatment in herpes simplex virus infection. | epicutaneous application of a contact sensitizer 2, 4-dinitro-1-chlorobenzene (dncb) is known to cause an inflammatory response and a hapten-specific t cell activation. we developed a highly stable gel formulation for dncb which is easy to apply to the skin. the present study focused on whether topical application of dncb gel produced immunomodulation with prophylactic effects against lethal herpes simplex virus infection (hsv) type 1. our results indicated that topical treatment with dncb gel i ... | 2009 | 19362169 |
| prevention of genital herpes simplex virus type 1 and 2 disease in mice immunized with a gd-expressing dominant-negative recombinant hsv-1. | cj9-gd is a novel herpes simplex virus (hsv) type 1 recombinant virus that is completely replication-defective, expresses high-levels of hsv-1 major antigen glycoprotein d (gd), and can function in trans to inhibit replication of wild-type hsv-1 and hsv-2 in co-infected cells. here, we show that immunization with cj9-gd elicits strong and long-lasting humoral and th1-like cellular immune responses against both hsv-1 and hsv-2. mice immunized with cj9-gd exhibited significant reductions in the ex ... | 2009 | 19357711 |
| physiological transgene regulation and functional complementation of a neurological disease gene deficiency in neurons. | the microtubule-associated protein tau (mapt) and alpha-synuclein (snca) genes play central roles in neurodegenerative disorders. mutations in each gene cause familial disease, whereas common genetic variation at both loci contributes to susceptibility to sporadic neurodegenerative disease. here, we demonstrate exquisite gene regulation of the human mapt and snca transgene loci and functional complementation in neuronal cell cultures and organotypic brain slices using the herpes simplex virus ty ... | 2009 | 19352323 |
| stepwise evolution of the herpes simplex virus origin binding protein and origin of replication. | the herpes simplex virus replicon consists of cis-acting sequences, oris and oril, and the origin binding protein (obp) encoded by the ul9 gene. here we identify essential structural features in the initiator protein obp and the replicator sequence oris, and we relate the appearance of these motifs to the evolutionary history of the alphaherpesvirus replicon. our results reveal two conserved sequence elements in herpes simplex virus type 1, obp; the rvknl motif, common to and specific for all al ... | 2009 | 19351883 |
| [effect of deletion mutant ips-1 on ifn-beta induction and its antiviral to hsv-1]. | the deletion mutant gene of ifn-beta promoter stimulator 1(deltaips-1) was constructed by depleting the amino acid region from 300 to 444 loci and the recombinant plasmid pef-bos-flag/deltaips-1 were constructed to study the effect of deletion mutant deltaips-1 to ifn-beta induction and its antiviral to hsv-1. | 2009 | 19351494 |
| cross-presentation of viral and self antigens by skin-derived cd103+ dendritic cells. | skin-derived dendritic cells (dcs) include langerhans cells, classical dermal dcs and a langerin-positive cd103(+) dermal subset. we examined their involvement in the presentation of skin-associated viral and self antigens. only the cd103(+) subset efficiently presented antigens of herpes simplex virus type 1 to naive cd8(+) t cells, although all subsets presented these antigens to cd4(+) t cells. this showed that cd103(+) dcs were the migratory subset most efficient at processing viral antigens ... | 2009 | 19349986 |
| role of chromatin during herpesvirus infections. | dna viruses have long served as model systems to elucidate various aspects of eukaryotic gene regulation, due to their ease of manipulation and relatively low complexity of their genomes. in some cases, these viruses have revealed mechanisms that are subsequently recognized to apply also to cellular genes. in other cases, viruses adopt mechanisms that prove to be exceptions to the more general rules. the double-stranded dna viruses that replicate in the eukaryotic nucleus typically utilize the h ... | 2009 | 19344747 |
| towards fibroid gene therapy: adenovirus-mediated delivery of herpes simplex virus 1 thymidine kinase gene/ganciclovir shrinks uterine leiomyoma in the eker rat model. | the objective of this study was to assess in vivo gene therapy of uterine leiomyomas in the eker rat model using adenovirus (ad)-mediated delivery of herpes simplex virus 1 thymidine kinase gene (hsv1tk) followed by ganciclovir (gcv) treatment. | 2009 | 19325244 |
| regulation of histone deposition on the herpes simplex virus type 1 genome during lytic infection. | during lytic infection by herpes simplex virus type 1 (hsv-1), histones are present at relatively low levels on the viral genome. however, the mechanisms that account for such low levels--how histone deposition on the viral genome is blocked or how histones are removed from the genome--are not yet defined. in this study, we show that histone occupancy on the viral genome gradually increased with time when transcription of the viral immediate-early (ie) genes was inhibited either by deletion of t ... | 2009 | 19321615 |
| arginine methylation of the icp27 rgg box regulates icp27 export and is required for efficient herpes simplex virus 1 replication. | the herpes simplex virus 1 (hsv-1) multifunctional regulatory protein icp27 shuttles between the nucleus and cytoplasm in its role as a viral mrna export factor. arginine methylation on glycine- and arginine-rich motifs has been shown to regulate protein export. icp27 contains an rgg box and has been shown to be methylated during viral infection. we found by mass spectrometric analysis that three arginine residues within the rgg box were methylated. viral mutants with substitutions of lysine for ... | 2009 | 19321610 |
| autophagy enhances the presentation of endogenous viral antigens on mhc class i molecules during hsv-1 infection. | viral proteins are usually processed by the 'classical' major histocompatibility complex (mhc) class i presentation pathway. here we showed that although macrophages infected with herpes simplex virus type 1 (hsv-1) initially stimulated cd8(+) t cells by this pathway, a second pathway involving a vacuolar compartment was triggered later during infection. morphological and functional analyses indicated that distinct forms of autophagy facilitated the presentation of hsv-1 antigens on mhc class i ... | 2009 | 19305394 |
| identification of multiple sites suitable for insertion of foreign genes in herpes simplex virus genomes. | information on sites in hsv genomes at which foreign gene(s) can be inserted without disrupting viral genes or affecting properties of the parental virus are important for basic research on hsv and development of hsv-based vectors for human therapy. the intergenic region between hsv-1 ul3 and ul4 genes has been reported to satisfy the requirements for such an insertion site. the ul3 and ul4 genes are oriented toward the intergenic region and, therefore, insertion of a foreign gene(s) into the re ... | 2009 | 19302526 |
| characterization of an immediate splenic precursor of cd8+ dendritic cells capable of inducing antiviral t cell responses. | mouse spleens contain three major dendritic cell (dc) populations: plasmacytoid dc, conventional cd8(+)cd24(+) dc (cd8(+) dc), and conventional cd8(-)cd24(-) dc (cd8(-) dc). we have previously shown that cd8(+) dc are the major cross-presenting subtype in vivo and are the main inducers of antiviral cytotoxic t lymphocyte responses. here we show that after depletion of cd8(+) dc, the only dc capable of viral ag presentation was a small subset that expresses cd24 but not cd8. this cd8(-)cd24(+) dc ... | 2009 | 19299718 |
| phenotypic similarities and differences between ul37-deleted pseudorabies virus and herpes simplex virus type 1. | in the absence of the tegument protein pul37, virion formation of pseudorabies virus (prv) and herpes simplex virus type 1 (hsv-1) is severely impaired. non-enveloped nucleocapsids accumulate in clusters in the cytoplasm, whereas only a few enveloped particles can be detected. although a contribution of pul37 to nuclear egress of hsv-1 has been suggested, the nuclear stages of morphogenesis are not impaired in prv-deltaul37-infected cells. moreover, hsv-1 pul37 has been described as essential fo ... | 2009 | 19297610 |
| extreme susceptibility of african naked mole rats (heterocephalus glaber) to experimental infection with herpes simplex virus type 1. | herpes simplex virus type 1 (hsv1) is widely used as a gene delivery vector in a variety of laboratory animals. in a recent study, a thymidine-kinase-inactive (replication-conditional) hsv1 used as a delivery vector was lethal in naked mole rats, whereas mice infected with the identical virus showed no adverse effects. this result prompted us to undertake a controlled comparative histologic study of the effect of hsv1 infection on naked mole rats and mice. replication-competent and replication-c ... | 2009 | 19295058 |
| kinetics of primary and memory cytotoxic t lymphocyte responses to herpes simplex virus 1 infection: granzyme b mediated ctl activity. | herpes simplex virus type 1 is one of the most common viruses among human population. studies demonstrate the essential role of cell mediated immunity, especially cd8+ t cells, in prevention and clearance of hsv1. | 2009 | 19293474 |
| isolation and preliminary characterization of herpes simplex virus 1 primary enveloped virions from the perinuclear space. | herpes simplex virus 1 (hsv-1) nucleocapsids exit the nucleus by budding into the inner nuclear membrane, where they exist briefly as primary enveloped virions. these virus particles subsequently fuse their envelopes with the outer nuclear membrane, permitting nucleocapsids to then enter the cytoplasm and complete assembly. we have developed a method to isolate primary enveloped virions from hsv-1-infected cells and subjected the primary enveloped virion preparation to maldi-ms/ms (matrix-assist ... | 2009 | 19279117 |
| virion incorporation of the herpes simplex virus type 1 tegument protein vp22 occurs via glycoprotein e-specific recruitment to the late secretory pathway. | the mechanism by which herpesviruses acquire their tegument is not yet clear. one model is that outer tegument proteins are recruited by the cytoplasmic tails of viral glycoproteins. in the case of herpes simplex virus tegument protein vp22, interactions with the glycoproteins ge and gd have been shown. we have previously shown that the c-terminal half of vp22 contains the necessary signal for assembly into the virus. here, we show that during infection vp22 interacts with ge and gm, as well as ... | 2009 | 19279114 |
| herpes simplex virus type 1 glycoprotein e mediates retrograde spread from epithelial cells to neurites. | in animal models of infection, glycoprotein e (ge) is required for efficient herpes simplex virus type 1 (hsv-1) spread from the inoculation site to the cell bodies of innervating neurons (retrograde direction). retrograde spread in vivo is a multistep process, in that hsv-1 first spreads between epithelial cells at the inoculation site, then infects neurites, and finally travels by retrograde axonal transport to the neuron cell body. to better understand the role of ge in retrograde spread, we ... | 2009 | 19279108 |
| [update on the treatment of genital herpes]. | genital herpes is a chronic infection characterized by periodic reactivation. it can produce symptomatic disease in the host although asymptomatic viral excretion can also occur. it is currently the main cause of genital ulceration and an important public health problem that has substantial clinical, psychological, and economic repercussions. this review analyzes the currently available therapeutic options and regimens, which are based mainly on systemic use of antiviral agents such as aciclovir ... | 2009 | 19268108 |
| herpes simplex virus type 1 regulatory protein icp0 aids infection in cells with a preinduced interferon response but does not impede interferon-induced gene induction. | several independent lines of evidence indicate that interferon-mediated innate responses are involved in controlling herpes simplex virus type 1 (hsv-1) infection and that the viral immediate-early regulatory protein icp0 augments hsv-1 replication in interferon-treated cells. however, this is a complex situation in which the experimental outcome is determined by the choice of multiplicity of infection and cell type and by whether cultured cells or animal models are used. it is now known that ne ... | 2009 | 19264774 |
| the epstein-barr virus alkaline exonuclease bglf5 serves pleiotropic functions in virus replication. | the epstein-barr virus (ebv) alkaline exonuclease bglf5 has previously been recognized to contribute to immune evasion by downregulating production of hla molecules during virus replication. we have constructed a bglf5-null virus mutant to determine bglf5's functions during ebv viral replication. quantification of virus production in permissive 293 cells carrying a deltabglf5 genome identified a 17- to 21-fold reduction relative to complemented or wild-type controls. detailed monitoring of delta ... | 2009 | 19264771 |
| [fullevir: in vitro and in vivo antiherpetic activity]. | the antiherpetic properties of a fullerene derivative with aminocaproic acid (manufactured by intelfarm co. as fullevir) were studied in in vitro (in sensitive cell cultures) and in vivo (on a murine model of experimental herpetic encephalitis) experiments. fullevir was found to protect tissue culture cells from the cytodestructive action of herpes simplex virus type 1. it was estimated that ed50 = 5.3 microg/ml and ed90 = 29.1 microg/ml. the agent was most effective when it was administered bef ... | 2009 | 19253725 |
| entry of herpes simplex virus 1 and other alphaherpesviruses via the paired immunoglobulin-like type 2 receptor alpha. | herpes simplex virus 1 (hsv-1) enters cells either via fusion of the virion envelope and host cell plasma membrane or via endocytosis, depending on the cell type. in the study reported here, we investigated a viral entry pathway dependent on the paired immunoglobulin-like type 2 receptor alpha (pilralpha), a recently identified entry coreceptor for hsv-1 that associates with viral envelope glycoprotein b (gb). experiments using inhibitors of endocytic pathways and ultrastructural analyses of chi ... | 2009 | 19244335 |
| detection of herpes viruses in children with acute appendicitis. | this study aimed to investigate the incidence of herpes simplex virus (hsv) types-1 and -2, varicella-zoster virus (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv), human herpes virus 6 (hhv-6) and human herpes virus 7 (hhv-7) in childhood acute appendicitis. | 2009 | 19233720 |
| viral supraglottitis in an adult. | 2009 | 19224474 | |
| molecular-genetic pet imaging using an hsv1-tk mutant reporter gene with enhanced specificity to acycloguanosine nucleoside analogs. | imaging 2 different molecular-genetic events in a single subject by pet is essential in a variety of in vivo applications. using herpes simplex virus-1 thymidine kinase (hsv1-tk) mutants with narrower substrate specificities in combination with wild-type hsv1-tk (wthsv1-tk) would enable differential imaging with corresponding radiotracers, namely 2'-deoxy-2'-(18)f-fluoro-5-ethyl-1-beta-d-arabinofuranosyl-uracil ((18)f-feau) and the acycloguanosine derivative 9-(4-(18)f-fluoro-3-[hydroxymethyl]bu ... | 2009 | 19223410 |
| in vitro antioxidant, antimicrobial and anti-herpes simplex virus type 1 activity of phellodendron amurense rupr. from china. | phellodendron amurense rupr. bark extracts were examined for antioxidant activity, antimicrobial activity and antiviral activity on herpes simplex virus type 1 (hsv-1). ethanol extract showed higher content of both total phenolic and flavonoid than aqueous extract. in dpph (2, 2-diphenyl-1-picrylhydrazyl) assay, the concentration providing 50% inhibition (ic(50)) values were 6.73 +/- 0.87 mg/ml and 4.26 +/- 0.59 mg/ml for aqueous and ethanol extracts respectively. ethanol extract had a much high ... | 2009 | 19222122 |
| alzheimer's disease-specific tau phosphorylation is induced by herpes simplex virus type 1. | neurofibrillary tangles are one of the main neuropathological features of alzheimer's disease (ad) and are composed of abnormally phosphorylated forms of a microtubule-associated protein called tau. what causes this abnormal phosphorylation is unknown. our previous studies have implicated herpes simplex virus type 1 (hsv1) as an etiological agent in ad, and so we investigated whether infection with this virus induces ad-like tau phosphorylation. here we demonstrate that hsv1 causes tau phosphory ... | 2009 | 19221424 |
| fewer latent herpes simplex virus type 1 and cytotoxic t cells occur in the ophthalmic division than in the maxillary and mandibular divisions of the human trigeminal ganglion and nerve. | following primary infection of the mouth, herpes simplex virus type 1 (hsv-1) travels retrogradely along the maxillary (v2) or mandibular (v3) nerve to the trigeminal ganglion (tg), where it establishes lifelong latency. symptomatic hsv-1 reactivations frequently manifest as herpes labialis, while ocular hsv-1 disease is rare. we investigated whether these clinical observations are mirrored by the distribution of latent hsv-1 as well as cytotoxic t-cell infiltration around the nerve cell bodies ... | 2009 | 19211753 |
| effects of herpes simplex virus amplicon transduction on murine dendritic cells. | the herpes simplex virus (hsv)-based amplicon is a versatile vaccine platform that has been preclinically vetted as a gene-based immunotherapeutic for cancer, hiv, and neurodegenerative disorders. although it is well known that injection of dendritic cells (dcs) transduced ex vivo with helper virus-free hsv amplicon vectors expressing disease-relevant antigens induces antigen-specific immune responses, the cellular receptor(s) by which the amplicon virion gains entry into dcs, as well as the eff ... | 2009 | 19199821 |
| [bell's palsy: from viral aetiology to diagnostic reality]. | peripheral injury of the facial nerve is a frequent disorder. it is a stressful situation for the patient and it is functionally hazardous for the cornea. facial palsy is due to a lesion involving the facial pontine nucleus or the nerve trunk in its route from the pontocerebellar angle to the parotid. the idiopathic facial paralysis or bell's palsy (bp) is the most common cause but acute facial palsy can also be due to tumors. a rigorous clinical history and examination must be performed to guid ... | 2009 | 19195745 |
| influence of antisense oligonucleotides targeting tumor necrosis factor-alpha on experimental herpetic-induced chorioretinitis of mouse eye. | to investigate the effect on the pathological process of topical treatment with antisense oligonucleotides (ason) targeting tumor necrosis factor-alpha (tnf-alpha) in experimental herpes simplex virus type-1 (hsv-1)-induced chorioretinitis of mouse eye. | 2009 | 19188750 |
| gsk983: a novel compound with broad-spectrum antiviral activity. | gsk983, a novel tetrahydrocarbazole, inhibits the replication of a variety of unrelated viruses in vitro with ec(50) values of 5-20 nm. both replication of the adenovirus ad-5 and the polyoma virus sv-40, and episomal maintenance of human papillomaviruses (hpv) and epstein-barr virus (ebv) are susceptible to gsk983. the compound does not inhibit all viruses; herpes simplex virus (hsv-1), human immunodeficiency virus (hiv), and lytic replication of ebv were not susceptible at concentrations below ... | 2009 | 19187793 |
| herpes simplex virus utilizes the large secretory vesicle pathway for anterograde transport of tegument and envelope proteins and for viral exocytosis from growth cones of human fetal axons. | axonal transport of herpes simplex virus (hsv-1) is essential for viral infection and spread in the peripheral nervous system of the host. therefore, the virus probably utilizes existing active transport and targeting mechanisms in neurons for virus assembly and spread from neurons to skin. in the present study, we used transmission immunoelectron microscopy to investigate the nature and origin of vesicles involved in the anterograde axonal transport of hsv-1 tegument and envelope proteins and o ... | 2009 | 19176621 |
| herpesvirus gb-induced fusion between the virion envelope and outer nuclear membrane during virus egress is regulated by the viral us3 kinase. | herpesvirus capsids collect along the inner surface of the nuclear envelope and bud into the perinuclear space. enveloped virions then fuse with the outer nuclear membrane (nm). we previously showed that herpes simplex virus (hsv) glycoproteins gb and gh act in a redundant fashion to promote fusion between the virion envelope and the outer nm. hsv mutants lacking both gb and gh accumulate enveloped virions in herniations, vesicles that bulge into the nucleoplasm. earlier studies had shown that h ... | 2009 | 19158241 |
| erythema nodosum-like lesions in treated whipple's disease: signs of immune reconstitution inflammatory syndrome. | treatment of systemic infections due to mycobacteria and hiv infection can lead to paradoxical worsening, the immune reconstitution inflammatory syndrome, in a minority of patients. herein we describe a patient with whipple's disease, a chronic systemic inflammatory disease caused by tropheryma whipplei, who developed cutaneous and later ocular disease after initiation of antibiotic therapy. a 42-year-old man with a 12-year history of arthralgias presented with deteriorating health, including we ... | 2009 | 19150271 |
| the bipolar filaments formed by herpes simplex virus type 1 ssb/recombination protein (icp8) suggest a mechanism for dna annealing. | herpes simplex virus type 1 encodes a multifunctional protein, icp8, which serves both as a single-strand binding protein and as a recombinase, catalyzing reactions involved in replication and recombination of the viral genome. in the presence of divalent ions and at low temperature, previous electron microscopic studies showed that icp8 will form long left-handed helical filaments. here, electron microscopic image reconstruction reveals that the filaments are bipolar, with an asymmetric unit co ... | 2009 | 19138689 |
| cell cycle inhibitor enhances the resolution of hsv-1-induced proinflammatory response in murine microglial cells. | herpes simplex encephalitis remains one of the most devastating intracranial infections despite available antiviral treatment, with sequelae secondary to a persistent inflammatory response. recently, cyclin-dependent kinases (cdks) have been found to act as cellular targets for antiviral drugs. however, the pharmacological effects of cdk inhibitors on glial cell function in herpes simplex encephalitis have not been elucidated. the aim of this work was to determine the influence of olomoucine on ... | 2009 | 19138471 |
| a case of maternal herpes simplex virus encephalitis during late pregnancy. | a pregnant 25-year-old woman at 32 weeks' gestation was admitted to an emergency unit after her husband had found her drowsy and with her tongue bitten. the day before admission, the patient had developed a fever of 39 degrees c, was suffering from headaches, was nauseated and had vomited. on admission, she had anterograde and retrograde amnesia, but no somatic neurological deficits were detected. | 2009 | 19129790 |
| kinetics of major histocompatibility class i antigen presentation in acute infection. | ag presentation within the regional lymph node is crucial for the initiation of cd8+ t cell responses following viral infection. the magnitude and quality of the cd8+ t cell response are regulated by the interplay between the size of the apc population and duration of ag presentation. to understand how these parameters are finely regulated during an immune response, we have investigated the dynamics of ag presentation in influenza a virus and hsv-1 infection. in both infections, apc production w ... | 2009 | 19124733 |
| hsv-1 infection through inhibitory receptor, pilralpha. | paired receptors that consist of highly related activating and inhibitory receptors are widely involved in the regulation of immune response. several viruses that persistently infect hosts possess genes that encode ligands for inhibitory receptors in order to escape from host immune system. herpes simplex virus type 1 (hsv-1) is one of the viruses that cause persistent infection. here, we found that hsv-1-infected cells express a ligand for paired immunoglobulin like-type 2 receptor (pilr)alpha, ... | 2008 | 19122386 |
| hsv immune complex (hsv-igg: ic) and hsv-dna elicit the production of angiogenic factor vegf and mmp-9. | angiogenesis and inflammatory mediators are critical pathogenic factors in herpetic stromal keratitis (hsk). since disease progresses without infectious virus, hsv-dna and hsv-igg complexes (hsv-ic) may contribute to hsk by triggering these factors. production of vegf and mmp-9 was studied in vitro using corneal epithelial cells (hce), fibroblasts (hcrf) and macrophages (thp-1). vegf was elevated in hcrf and thp-1 following treatment with hsv-dna and hsv-ic. mmp-9 was elevated in thp-1 but not i ... | 2009 | 19115032 |
| american college health association annual pap test and sexually transmitted infection survey: 2006. | the authors describe the cervical cytology and sexually transmitted infection (sti) testing patterns of us college health centers. | 2009 | 19114378 |
| host responses to wild-type and attenuated herpes simplex virus infection in the absence of stat1. | humans and mice lacking the interferon signaling molecule stat1 are sensitive to a variety of pathogens due to their presumed inability to mount a strong innate immune response. the herpes simplex virus type 1 (hsv-1) virion host shutoff (vhs) protein is a multifunctional immunomodulator that counteracts the innate immune response and viruses lacking vhs are attenuated and effective live vaccines in animal models. to investigate the interplay of viruses with an immunocompromised host, we perform ... | 2009 | 19109391 |
| acute retinal necrosis in primary herpes simplex virus type i infection. | here we report the case of an immunocompetent 8-year-old child who developed acute retinal necrosis concomitant with a primary herpes simplex virus type i infection. ocular inflammation changed along with the development of a specific antibody titer in the serum. this evidence suggests that the immune response of the host can significantly modulate the clinical aspect of the ocular infection. | 2009 | 19106778 |
| [analgesic effect of intrathecal injection of herpes simplex virus type i amplicon vector-mediated human preproenkephalin gene on chronic neuropathic pain: experiment with rats]. | to investigate the antinociceptive effect of intrathecal (it) injection of herpes simplex virus type i (hsv-1) amplicon vector-mediated hppe on chronic neuropathic pain. | 2008 | 19080437 |
| herpes simplex virus type 1 thymidine kinase sequence fused to the lacz gene increases levels of {beta}-galactosidase activity per genome of high-capacity but not first-generation adenoviral vectors in vitro and in vivo. | increased transgene expression per vector genome is an important goal in the optimization of viral vectors for gene therapy. herein we demonstrate that herpes simplex virus type 1 (hsv1) thymidine kinase (tk) gene sequences (1,131 bp) fused to the 3' end of lacz increase transgene expression from high-capacity adenoviral vectors (hcad), but not from first-generation (ad) vectors. the woodchuck hepatitis virus posttranscriptional regulatory element (wpre), in contrast, increased transgene express ... | 2009 | 19073729 |
| a serological survey of herpes simplex virus type 1 and 2 immunity in pregnant women at labor stage in tehran, iran. | this study was carried out to determine the prevalence of neutralizing antibodies to herpes simplex virus type 1 (hsv-i) and type 2 (hsv-2) in pregnant women at labor stage. blood samples from umbilical cord of four hundred women aging 16 to 40 years at labor stage were collected. after sera separation quantity of anti hsv-1 and hsv-2 antibodies were measured by serum neutralization test. antibody quantification was assayed by two fold dilution of sera (from 1/2 to 1/256) with 500 tissue culture ... | 2007 | 19070003 |
| detection of herpesviruses and periodontal pathogens in subgingival plaque of patients with chronic periodontitis, generalized aggressive periodontitis, or gingivitis. | herpesviruses may be related to the etiology of aggressive periodontitis (agp) and chronic periodontitis (cp) by triggering periodontal destruction or by increasing the risk for bacterial infection. this case-control study evaluated the presence of herpes simplex virus type i (hsv-1), epstein-barr virus type i (ebv-1), human cytomegalovirus (hcmv), aggregatibacter actinomycetemcomitans (previously actinobacillus actinomycetemcomitans), porphyromonas gingivalis, prevotella intermedia, and tannere ... | 2008 | 19053922 |
| nuclear import of hsv-1 dna polymerase processivity factor ul42 is mediated by a c-terminally located bipartite nuclear localization signal. | the polymerase accessory protein of the human herpes simplex virus type 1 (hsv-1) dna polymerase ul42 plays an essential role in viral replication, conferring processivity to the catalytic subunit ul30. we show here that ul42 is imported to the nucleus of living cells in a ran- and energy-dependent fashion, through a process that requires a c-terminally located bipartite nuclear localization signal (ul42-nlsbip; pttkrgrsggedaradalkkpk(413)). moreover cytoplasmic mutant derivatives of ul42 lackin ... | 2008 | 19053255 |
| dynamic of migration of hsv-1 from a medullary pronociceptive centre: antinociception by overexpression of the preproenkephalin transgene. | herpes simplex virus type 1 (hsv-1) vectors are known to inhibit nociceptive transmission at the spinal cord after peripheral applications. similar approaches may also be useful when applied at the supraspinal pain control system as the system includes pronociceptive (facilitatory) components. we performed a study aimed to analyse the migration of hsv-1 along with the inhibition of pronociception from the medullary dorsal reticular nucleus (drt), a major facilitatory component of the supraspinal ... | 2008 | 19046388 |
| delivery of interferon-gamma by an adenovirus vector blocks herpes simplex virus type 1 reactivation in vitro and in vivo independent of rnase l and double-stranded rna-dependent protein kinase pathways. | hsv-1 is a significant human pathogen that can result in the loss of sight as a result of episodic reactivation of latent virus from sensory ganglion neurons. in this study the potential efficacy of anti-viral cytokine expression in preventing latent virus reactivation was investigated. both type i (ifn-beta) and type ii (ifn-gamma) ifn transgene expression following transduction of trigeminal ganglion explant cultures significantly reduced the incident of hsv-1 reactivation that in the case of ... | 2009 | 19042034 |
| [an experimental study on targeting suicide gene therapy for lung cancer with hsv-tk driven by htert promoter]. | to study the approach of targeting expression of suicide gene hsv-tk driven by human telomerase catalytic subunit (htert) promoter in lung cancer cells, and to investigate inhibitory effect of hsv-tk/gcv driven by htert promoter on proliferation of lung cancer cell line a549 in vitro and in vivo. | 2008 | 19024294 |
| efficient nuclear export of herpes simplex virus 1 transcripts requires both rna binding by icp27 and icp27 interaction with tap/nxf1. | herpes simplex virus 1 (hsv-1) regulatory protein icp27 has been reported to bind viral rna and to interact with the nuclear export adaptor aly/ref and the major cellular mrna nuclear export receptor tap/nxf1. using in situ hybridization and in vitro export assays, we show here that poly(a)(+) rna was retained in the nucleus of cells infected with viral icp27 mutants that either cannot bind rna or that do not interact with tap/nxf1. microarray analysis of nuclear and cytoplasmic rna fractions de ... | 2009 | 19019956 |
| loop ii of dna polymerase beta is important for discrimination during substrate binding. | loop ii of dna polymerase beta (pol beta) consists of 14 amino acid residues and is highly flexible and solvent exposed. previous research from our laboratory has shown that this loop is important for polymerase activity and fidelity. in the study presented here, we demonstrate that a shortened five amino acid residue loop compromises the fidelity of pol beta. this five-residue loop, termed eneyp, induces one base frameshift errors and a-c transversions within a specific sequence context. we dem ... | 2009 | 19013261 |
| high-level expression of glycoprotein d by a dominant-negative hsv-1 virus augments its efficacy as a vaccine against hsv-1 infection. | using the t-rex (invitrogen, carlsbad, ca) gene switch technology, we previously generated a dominant-negative herpes simplex virus (hsv)-1 recombinant, cj83193, capable of inhibiting its own replication as well as that of wild-type hsv-1 and hsv-2. it has been further demonstrated that cj83193 is an effective vaccine against hsv-1 infection in a mouse ocular model. to ensure its safety and augment its efficacy, we generated an improved cj83193-like hsv-1 recombinant, cj9-gd, which contains a de ... | 2009 | 19005489 |
| [specific cellular immune system and a human herpes virus infection in various forms of tubercular process]. | tuberculosis (tb) is one of the most common infective diseases in history. in industrialized nations tb was well on its way to becoming extinct, however, tb rates started increasing again in the majority of developed countries today. there is a need to further understanding the role of a human herpes virus (hhv) in development and prognosis of tb. the goal of research was to study a condition of the specific immune system, frequency of activization of a hhv infection of 1 type and presence of in ... | 2008 | 18997252 |
| ras signaling influences permissiveness of malignant peripheral nerve sheath tumor cells to oncolytic herpes. | lack of expression of neurofibromin in neurofibromatosis 1 and its lethal derivative, malignant peripheral nerve sheath tumors (mpnsts), is thought to result in the overactivation of the ras signaling pathway. our previous studies have shown that cells with overactivation in the ras pathway are more permissive to infection with herpes simplex virus 1 and its mutant version r3616. in this study, we show that among five different mouse mpnst cell lines, only the ones with elevated levels of ras si ... | 2008 | 18988803 |
| magnetic resonance and diffusion-weighted imaging findings of herpes simplex encephalitis. | herpes simplex encephalitis (hse) is a rare, life-threatening disease. this paper draws attention to the role of imaging in early hse diagnosis. five consecutive patients diagnosed with hse (type 1) between june 2005 and june 2006 (three males, two females, mean age 44 [range 16-68] years) were included in this retrospective study. computed tomography, conventional magnetic resonance imaging (mri) sequences and diffusion-weighted imaging (dwi) were obtained for each patient. apparent diffusion c ... | 2008 | 18983763 |
| [facial nerve paresis]. | 2008 | 18983050 | |
| tumor-specific activity of cellular regulatory elements is down-regulated upon insertion into the herpes simplex virus genome. | transcriptional targeting of viral genes is a promising strategy to achieve tumor-specific replication of oncolytic viruses. due to its natural tropism, herpes simplex virus type 1 (hsv-1) may be an ideal tool for oncolytic therapy of brain tumors such as malignant glioblastoma. to study whether glioma-specific gene expression can be accomplished within the hsv-1 genome, four cellular regulatory elements were exemplarily studied. whereas the human telomerase reverse transcriptase (htert) and sur ... | 2008 | 18979278 |
| herpes simplex virus type 1 dna is located within alzheimer's disease amyloid plaques. | the brains of alzheimer's disease sufferers are characterized by amyloid plaques and neurofibrillary tangles. however, the cause(s) of these features and those of the disease are unknown, in sporadic cases. we previously showed that herpes simplex virus type 1 is a strong risk factor for alzheimer's disease when in the brains of possessors of the type 4 allele of the apolipoprotein e gene (apoe-epsilon4), and that beta-amyloid, the main component of plaques, accumulates in herpes simplex virus t ... | 2009 | 18973185 |
| differing roles of inner tegument proteins pul36 and pul37 during entry of herpes simplex virus type 1. | studies with herpes simplex virus type 1 (hsv-1) have shown that secondary envelopment and virus release are blocked in mutants deleted for the tegument protein gene ul36 or ul37, leading to the accumulation of dna-containing capsids in the cytoplasm of infected cells. the failure to assemble infectious virions has meant that the roles of these genes in the initial stages of infection could not be investigated. to circumvent this, cells infected at a low multiplicity were fused to form syncytia, ... | 2009 | 18971278 |
| structure-function analysis of herpes simplex virus glycoprotein b with fusion-from-without activity. | fusion-from-without (ffwo) is the rapid induction of cell fusion by virions in the absence of viral protein synthesis. the combination of two amino acid mutations in envelope glycoprotein b (gb), one in the ectodomain and one in the cytoplasmic tail, can confer ffwo activity to wild type herpes simplex virus (hsv). in this report, we analyzed the entry and cell fusion phenotypes of hsv that contains ffwo gb, with emphasis on the cellular receptors for hsv, nectin-1, nectin-2 and hvem. the abilit ... | 2008 | 18950828 |
| the role of herpes simplex virus-1 thymidine kinase alanine 168 in substrate specificity. | herpes simplex virus type 1 (hsv) thymidine kinase (tk) has been widely used in suicide gene therapy for the treatment of cancer due to its broad substrate specificity and the inability of the endogenous human tk to phosphorylate guanosine analogs such as ganciclovir (gcv). the basis of suicide gene therapy is the introduction of a gene that encodes a prodrug-activating enzyme into tumor cells. after administration, the prodrug is selectively converted to a toxic drug by the suicide gene product ... | 2008 | 18949076 |
| the effects of trichostatin a on the oncolytic ability of herpes simplex virus for oral squamous cell carcinoma cells. | combining the use of a chemotherapeutic agent with oncolytic virotherapy is a useful way to increase the efficiency of the treatment of cancer. the effect of the histone diacetylase (hdac) inhibitor trichostatin a (tsa) on the antitumor activity of a herpes simplex virus type-1 (hsv-1) mutant was examined in oral squamous cell carcinoma (scc) cells. immunoblotting analysis and immunoflourescence staining revealed that a cytoplasmic nuclear factor-kappab (nf-kappab) component, p65, translocated i ... | 2009 | 18949013 |
| the two functions of herpes simplex virus 1 icp0, inhibition of silencing by the corest/rest/hdac complex and degradation of pml, are executed in tandem. | icp0, an alpha (immediate-early) protein of herpes simplex virus 1, performs at least two key functions. it blocks inhibition of viral-gene expression by interferon, a function dependent on the degradation of the nd10 components pml and sp100 by the ubiquitin ligase expressed by the ring finger (rf), and it blocks silencing of viral dna mediated by the hdac1/2-corest-rest complex. in the latter case, a mutant corest lacking the hdac1 binding site compensates totally or in part for the absence of ... | 2009 | 18945770 |
| [antiviral and immunomodulating activity of polyprenyl phosphates in viral infections]. | expressed antiviral activity of fortepren (fp) and gamapren (gp), polyprenyl phosphate (ppp)-contaning agents, was demonstrated in experiments on mice infected with the human herpes simplex virus, type 1 (hsv1) or the vernal encephalitis virus (vev). since both the viral infections are of great social significance, the ppp-containing agents should be considered prospective for the medical practice. the experimental data suggested that both the drugs considerably inhibited the vev infectiousness ... | 2008 | 18942418 |
| cutting edge: local recall responses by memory t cells newly recruited to peripheral nonlymphoid tissues. | infection results in the formation of a circulating effector memory t cell population able to enter peripheral tissues either in the steady state or in response to localized infection. as a consequence, recall is thought to result from a phased response first involving those t cells already at the site of infection followed by the infiltration of memory cells from the wider circulation. we have recently reported that tissue-resident t cells can undergo stimulation and proliferation in response t ... | 2008 | 18941171 |
| the product of the herpes simplex virus 1 ul7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2. | the herpes simplex virus 1 (hsv-1) ul7 gene is highly conserved among herpesviridae. since the construction of recombinant hsv-1 with a mutation in the ul7 gene has not been reported, the involvement of hsv-1 ul7 in viral replication has been unclear. in this study, we succeeded in generating a ul7 null hsv-1 mutant virus, mt102, and characterized it. our results were as follows. (i) in vero cells, mt102 was replication-competent, but formed smaller plaques and yielded 10- to 100-fold fewer prog ... | 2008 | 18940012 |
| the essential and non-essential genes of bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) is an economically important pathogen of cattle associated with respiratory and reproductive disease. to further develop bohv-1 as a vaccine vector, a study was conducted to identify the essential and non-essential genes required for in vitro viability. random-insertion mutagenesis utilizing a tn5 transposition system and targeted gene deletion were employed to construct gene disruption and gene deletion libraries, respectively, of an infectious clone of bohv-1. tra ... | 2008 | 18931083 |
| the p59 oligoadenylate synthetase-like protein possesses antiviral activity that requires the c-terminal ubiquitin-like domain. | viral infection of mammalian cells prompts the innate immune system to initiate an antiviral response. the recognition of the virus triggers several antiviral signalling pathways, which among others include the family of 2'-5' oligoadenylate synthetase (oas) proteins. the p59 protein encoded by the oas-like (oasl) gene is an atypical member of the oas family in the sense that it lacks the characteristic 2'-5' oligoadenylate synthetase activity. we decided to investigate the putative antiviral ac ... | 2008 | 18931074 |
| noninvasive molecular imaging of hypoxia in human xenografts: comparing hypoxia-induced gene expression with endogenous and exogenous hypoxia markers. | tumor hypoxia is important in the development and treatment of human cancers. we have developed a novel xenograft model for studying and imaging of hypoxia-induced gene expression. a hypoxia-inducible dual reporter herpes simplex virus type 1 thymidine kinase and enhanced green fluorescence protein (hsv1-tkegfp), under the control of hypoxia response element (9hre), was stably transfected into human colorectal ht29 cancer cells. selected clones were further enriched by repeated live cell sorting ... | 2008 | 18922936 |
| synthesis, antioxidative and antiviral activity of hydroxycinnamic acid amides of thiazole containing amino acid. | the synthesis and the biological (antioxidant and antiviral) activities of novel hydroxycinnamic acid amides of a thiazole containing tfa.valine-4-carboxylic acid ethyl ester are reported. the amides have been synthesized from p-coumaric, ferulic and sinapic acids with the corresponding tfa.valine-thiazole-4-carboxylic acid ethyl ester using the coupling reagent n-ethyl-n'-(3-dimethylaminopropyl) carbodiimide hydrochloride (edc) and 4-(dimethylamino) pyridine (dmap) as a catalyst. the antioxidan ... | 2009 | 18853101 |
| primary target cells of herpes simplex virus type 1 in the hippocampus. | herpes simplex virus type 1 (hsv-1) causes fatal and sporadic encephalitis in human. the encephalitis-survivors frequently suffer from symptoms of memory deficits. it remains unclear how hsv-1 induces tissue damages in memory formation-associated brain tissues such as the hippocampus. in this study, we examined hsv-1 infection in the hippocampus using a rat hsv-1 infection model. we found profound pathological changes in the hippocampus and large numbers of hsv-1 antigen-positive cells in the de ... | 2008 | 18852062 |
| structure-activity relationships for dipeptide prodrugs of acyclovir: implications for prodrug design. | a series of water-soluble dipeptide ester prodrugs of the antiviral acyclovir (acv) were evaluated for their chemical stability, cytotoxicity, and antiviral activity against several strains of herpes simplex-1 and -2, vaccinia, vesicular stomatitis, cytomegalovirus and varicella zoster viruses. acv dipeptide esters were very active against herpetic viruses, independently of the rate at which they liberate the parent drug. their minimum cytotoxic concentrations were above 100 microm and the resul ... | 2009 | 18848738 |
| herpes simplex-like infection in a bottlenose dolphin stranded in the canary islands. | a bottlenose dolphin, stranded in the canary islands in 2001 exhibited non-suppurative encephalitis. no molecular detection of cetacean morbillivirus (cemv) was found, but a herpesviral-specific band of 250 bp was detected in the lung and brain. the sequenced herpesviral pcr product was compared with genbank sequences, obtaining 98% homology (p-distance of 0.02) with human herpesvirus 1 (herpes simplex virus 1 or hsv-1). this is the first report of a herpes simplex-like infection in a stranded d ... | 2008 | 18828564 |
| [investigation of viral nucleic acids in middle-ear effusion specimens from children with acute otitis media]. | acute otitis media with effusion (ome) is one of the major causes of antibiotic use, indication for operation and hearing loss in children. in two third of the cases the etiologic agents are bacteria. nonetheless, increasing numbers of reports have implicated viruses as etiologic agents that may have some effect on prognosis of ome. the aim of this study was to investigate the presence of nucleic acids of respiratory syncytial virus (rsv) type a and b, influenza type a virus, adenovirus, cytomeg ... | 2008 | 18822887 |
| [investigation of herpes simplex virus in viral meningoencephalitis suspected cases using molecular and serological methods]. | herpes simplex virus (hsv) meningoencephalitis has a high mortality rate if proper antiviral therapy is not applied. thus rapid diagnosis is of peculiar importance in such cases. in this study we aimed to evaluate the use of polymerase chain reaction (pcr) and detection of intrathecally synthesized antibodies by serological methods in viral meningoencephalitis suspected cases to determine hsv as the causative agent. seventeen cases with cerebrospinal fluid (csf) samples with microscopical and bi ... | 2008 | 18822885 |
| role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells. | herpes simplex virus 1 (hsv-1) demonstrates a unique ability to infect a variety of host cell types. retinal pigment epithelial (rpe) cells form the outermost layer of the retina and provide a potential target for viral invasion and permanent vision impairment. here we examine the initial cellular and molecular mechanisms that facilitate hsv-1 invasion of human rpe cells. high-resolution confocal microscopy demonstrated initial interaction of green fluorescent protein (gfp)-tagged virions with f ... | 2008 | 18803666 |
| synthesis and in vitro evaluation of 5-[(18)f]fluoroalkyl pyrimidine nucleosides for molecular imaging of herpes simplex virus type 1 thymidine kinase reporter gene expression. | two novel series of 5-fluoroalkyl-2'-deoxyuridines (fprdu, fbudu, fpedu) and 2'-fluoro-2'-deoxy-5-fluoroalkylarabinouridines (ffprau, ffbuau, ffpeau) that have three, four, or five methylene units (propyl, butyl, or pentyl) at c-5 were prepared and tested as reporter probes for imaging herpes simplex virus type 1 thymidine kinase (hsv1- tk) gene expression. the negishi coupling methodology was employed in efficiently synthesizing the radiolabeling precursors. all six 5-[(18)f]fluoroalkyl pyrimid ... | 2008 | 18800764 |
| anti-hsv-1 and anti-hiv-1 activity of gallic acid and pentyl gallate. | the synthetic n-alkyl esters of gallic acid (ga), also known as gallates, especially propyl, octyl and dodecyl gallates, are widely employed as antioxidants by food and pharmaceutical industries. the inhibitory effects of ga and 15 gallates on herpes simplex virus type 1 (hsv-1) and human immunodeficiency virus (hiv-1) replication were investigated here. after a preliminary screening of these compounds, ga and pentyl gallate (pg) seemed to be the most active compounds against hsv-1 replication a ... | 2008 | 18797755 |
| [construction of herpes simplex virus-1 virion protein 22-mediated microdystrophin gene recombinant adenovirus and study on the property of protein transduction]. | to construct the recombinant adenovirus containing herpes simplex virus-1 virion protein (vp) 22 and human microdystrophin gene, then the adenovirus was transfected into c2c12 myoblast and studied on the property of protein transduction with vp22-mediated microdystrophin in c2c12 myoblast. | 2008 | 18795628 |