Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| effect of dietary lipoic acid on metabolic hormones and acute-phase proteins during challenge with infectious bovine rhinotracheitis virus in cattle. | to determine the effect of dietary supplemental lipoic acid (la) on serum concentrations of metabolic hormones and acute-phase proteins of steers challenged with infectious bovine rhinotracheitis virus (ibrv). | 2006 | 16817742 |
| induction of antigen-specific t-cell subset activation to bovine respiratory disease viruses by a modified-live virus vaccine. | to determine the efficacy of a modified-live virus vaccine containing bovine herpes virus 1 (bhv-1), bovine respiratory syncytial virus (brsv), parainfluenza virus 3, and bovine viral diarrhea virus (bvdv) types 1 and 2 to induce neutralizing antibodies and cell-mediated immunity in naïve cattle and protect against bhv-1 challenge. | 2006 | 16817740 |
| bovine herpesvirus 1 immediate-early protein (bicp0) interacts with the histone acetyltransferase p300, which stimulates productive infection and gc promoter activity. | the immediate-early protein, bicp0, of bovine herpesvirus 1 (bhv-1) transactivates viral promoters and stimulates productive infection. bicp0 is expressed constitutively during productive infection, as its gene contains an immediate-early and an early promoter. like other icp0 homologues encoded by members of the subfamily alphaherpesvirinae, bicp0 contains a zinc ring finger located near its n terminus. mutations that disrupt the bicp0 zinc ring finger impair its ability to activate transcripti ... | 2006 | 16760386 |
| antiviral effect of guazuma ulmifolia and stryphnodendron adstringens on poliovirus and bovine herpesvirus. | crude extract (ce) and aqueous (aqf) and ethyl acetate (etoacf) fractions of guazuma ulmifolia lam., sterculiaceae and the corresponding aqf, etoacf of stryphnodendron adstringens (mart.) coville, leguminosae were tested for their antiviral activity against poliovirus 1 (p-1) and bovine herpesvirus 1 (bhv-1) in hep-2 cultured cells. the antiviral activity was monitored by plaque assay and immunofluorescence assay (ifa) under virucidal and therapeutic protocols. the therapeutic protocol demonstra ... | 2006 | 16754999 |
| the in vitro antiviral activity of an aliphatic nitro compound from heteropteris aphrodisiaca. | we investigated the antiviral activity of an aliphatic nitro compound (nc) isolated from heteropteris aphrodisiaca o. mach. (malpighiaceae), a brazilian medicinal plant. the nc was tested for its antiviral activity against poliovirus type 1 (pv-1) and bovine herpes virus type 1 (bhv-1) by plaque reduction assay in cell culture. the nc showed a moderate antiviral activity against pv-1 and bhv-1 in hep-2 cells, and the 50% inhibitory concentration (ic50) were 22.01 microg/ml (selectivity index (si ... | 2008 | 16735108 |
| evaluation of a live trivalent vaccine for infectious bovine rhinotracheitis virus, para-influenza type-3 virus and pasteurella multocida in calves. | a live trivalent vaccine for infectious bovine rhinotracheitis virus (ibr), para-influenza type-3 virus (pi3) and pasteurella multocida type b vaccine was prepared. the preparation contained 10(7) tcid50 of ibr, 10(7) tcid50 of pi3 & 10(9) cfu of p. multocida type b per 3 ml. ten calves (5-7 days old) were vaccinated intranasally while five unvaccinated calves of the same age were used as control. vaccinated calves were examined daily for disease manifestation. serum and nasal secretion specimen ... | 2004 | 16734122 |
| bovine herpesvirus 1 ul49.5 protein inhibits the transporter associated with antigen processing despite complex formation with glycoprotein m. | bovine herpesvirus 1 (bhv-1) interferes with peptide translocation by the transporter associated with antigen processing (tap). recently, the ul49.5 gene product of bhv-1 was identified as the protein responsible for the observed inhibition of tap. in bhv-1-infected cells and virions, the ul49.5 protein forms a complex with glycoprotein m (gm). hence, it was investigated whether ul49.5 can combine the interactions with gm and the tap complex. in cell lines constitutively expressing both ul49.5 a ... | 2006 | 16731921 |
| susceptibility of zona-intact and zona-free in vitro-produced bovine embryos at different stages of development to infection with bovine herpesvirus-1. | the aim of the present study was to determine if bhv-1 is able to replicate within in vitro produced embryos and to investigate the degree to which the zona pellucida (zp) is able to protect in vitro produced embryos against infection with bhv-1. both zp-intact and zp-free matured oocytes, zygotes (1 d post insemination; 1dpi), 8-cell stage embryos (3 dpi), morulae (6 dpi) were incubated for 1 h in 1 ml of mem containing 10(7.7) tcid(50)/ml bhv-1 (cooper strain). three titers (10(5.7), 10(6.7) a ... | 1997 | 16728085 |
| in vitro fertilization and culture of ova from heifers infected with bovine herpesvirus-1 (bhv-1). | oocytes collected from heifers infected experimentally with bovine herpesvirus-1 (bhv-1, 10(8) tcid(50)/ml) and from dexamethasone-treated (stressed) bhv-1 seropositive animals were matured, fertilized and co-cultured in vitro for 7 d prior to being tested for the presence of the virus. nineteen of the 21 infected donors yielded embryos and follicular fluids that were bhv-1 positive. oviductal cells (17 21 ) and uterine fluids (14 21 ) were also positive. titers for the positive samples ranged 1 ... | 1994 | 16727474 |
| distribution of bhv1 in fractions of semen from a naturally infected bull. | the aim of the present study was to investigate the distribution of bhv1 (bovine herpesvirus 1) in the semen from a naturally infected bull and more particularly, to determine if there were viral particles associated with sperm that could interfere with in-vivo or in-vitro fertilization. frozen semen from a single ejaculate that had been previously identified as being contaminated with bhv1 was used. the contents of 12 ministraws were mixed. two aliquotes served as controls, the first was held a ... | 1993 | 16727382 |
| isolation of bovine herpesvirus-1 (bhv-1) and bovine viral diarrhea virus (bvdv) in association with the in vitro production of bovine embryos. | the purpose of this study was to determine whether oocytes obtained from bovine ovaries collected at commercial abattoirs for use in in vitro fertilization programs would be contaminated with bovine herpesvirus-1 (bhv-1) and/or bovine viral diarrhea virus (bvdv). in total, of 85 samples tested containing 759 embryos produced by in vitro fertilization, 2 (2.4%) were positive for bhv-1 while none were positive for bvdv. the follicular fluid collected during oocyte aspiration tested positive in 11. ... | 1993 | 16727336 |
| inactivation of bovine herpesvirus-1 and bovine viral diarrhea virus in association with preimplantation bovine embryos using photosensitive agents. | hematoporphyrin (hp), hematoporphyrin derivative (hpd), and thiopyronine (tp) are photosensitive agents (psa) that have a germicidal effect when they are activated by light: helium neon laser (he/ne) light (hp, hpd), white light (hp, hpd), and yellow-green light (tp). experiments were conducted with appropriate controls to determine the effect of photosensitive agents a) for inactivating bovine herpesvirus-1 (bhv-1; titre 10(6) tcid50/ml) and bovine viral diarrhea virus (bvdv; titre 10(6) tcid50 ... | 1992 | 16727166 |
| trypsin treatment of bovine embryos after in vitro exposure to infectious bovine rhinotracheitis virus or bovine herpesvirus-4. | the objectives of this study were to evaluate the efficacy of trypsin treatment for the removal/inactivation of infectious bovine rhinotracheitis virus (ibrv) adhering to zona pellucida-intact (zp-i) bovine embryos and to determine if bovine herpesvirus-4 (bhv-4) adheres to zp-i bovine embryos. when adherence of bhv-4 was demonstrated, an additional objective was to determine whether trypsin treatment removes or inactivates this virus. a total of 139 zp-i embryos was collected from superovulated ... | 1990 | 16726850 |
| effects of hormone-induced superovulation on latent infectious bovine rhinotracheitis virus in cattle. | twelve nulliparous, sexually mature heifers free of antibodies to infectious bovine rhinotracheitis (ibr) virus were exposed intranasally to colorado strain ibr virus. after 3 mos, when the postexposure antibody titers had stabilized, the heifers were divided into three groups. individuals in each group were treated with either saline, dexamethasone or follicle stimulating hormone (fsh) for five consecutive days. blood samples were taken at predetermined intervals for isolation of virus, and for ... | 1989 | 16726552 |
| inactivation of bovine herpesvirus-1 (bhv-i) from in vitro infected bovine semen. | frozen-thawed bovine semen, experimentally infected with bovine herpesvirus-1 (bhv-1) at levels of 10(3) tcid(50)/ml and 10(4) tcid(50)/ml, was treated with a 0.3% trypsin solution to determine the effect of trypsin on the virus and on fertilization using superovulated animals. virus was not isolated from any trypsin-treated samples using a cell culture assay system. nor did two calves develop antibodies to bhv-1 following inoculation with trypsin-treated semen pooled from six bulls. nonsurgical ... | 1988 | 16726507 |
| the in vitro exposure to bovine rhinotracheitis virus of zona pellucida-micromanipulated bovine embryos with the zona pellucida damaged or removed. | one hundred and eighty-five embryos were collected from 29 superovulated donors 6 to 8 d post estrus. the zona pellucida (zp) of these embryos was either cracked, removed mechanically or removed with acidified tyrode's solution, or left intact. forty-eight of 103 (47%) zp-cracked and zp-free embryos, exposed for 24 h to infectious bovine rhinotracheitis virus (ibrv), survived. no significant difference was found in the embryonic survival of the zp-cracked embryos exposed to ibrv and control embr ... | 1987 | 16726332 |
| prevalence of antibodies to specific infectious agents in bovine fetuses from a slaughterhouse in minnesota. | sera from 486 bovine fetuses, approximately 60 to 270 days of gestation, were collected at slaughter and tested for the presence of immunoglobulins (ig). one hundred ten (27%) of the sera were positive for igg and/or igm. the earliest age at which fetuses tested positive for igm and igg was estimated to be 100 and 120 days, respectively. ig concentration increased with increased age of the fetus. sera that were positive for ig were tested for the presence of specific antibodies to five different ... | 1985 | 16726037 |
| embryo transfer as a means of controlling the transmission of viral infections. ii. the in vitro exposure of preimplantation bovine embryos to infectious bovine rhinotracheitis virus. | bovine embryos, at the 16-cell to the blastocyst stage of development, were exposed to infectious bovine rhinotracheitis virus (ibrv) for either one or 24 hours. these embryos were then washed and incubated for 24 or 48 hours before being assayed for ibrv. under these conditions, infectious virus at the level of 0-10(2.2) tcid(50)/ml was isolated from 57-64% of the embryos exposed to ibrv. trypsin and ibrv-antiserum were found to be capable of removing and/or inactivating the ibrv from exposed e ... | 1982 | 16725735 |
| a method to test large numbers of bovine semen samples for viral contamination and results of a study using this method. | a procedure to test large numbers of semen samples for viral contamination is described. the procedure has the advantages of being simple to perform, sensitive and a relatively inexpensive method to test pooled semen samples, but it has the disadvantage of requiring serologically negative calves and sheep, isolation facilities to prevent environmental contamination during the testing procedure and relatively long times to obtain results. using this in vivo procedure called the "cornell semen tes ... | 1982 | 16725672 |
| a clinical, evidence-based approach to infectious causes of infertility in beef cattle. | infertility is the diminished or absent capacity to produce viable offspring. infections that reduce ovulation rates, fertilization rates, embryonic survival rates, fetal survival rates or perinatal survival rates result in observed infertility in beef cows. reproductive pathogens include leptospira, campylobacter, hemophilus, brucella, bovine herpesvirus-1, bovine viral diarrhea virus, tritrichomonas foetus, and neospora caninum. infectious infertility can be prevented or controlled with approp ... | 2006 | 16725187 |
| the cell-mediated immune response induced by plasmid encoding bovine herpesvirus 1 glycoprotein b is enhanced by plasmid encoding il-12 when delivered intramuscularly or by gene gun, but not after intradermal injection. | bovine herpesvirus 1 (bhv-1) causes respiratory and genital infections in cattle. previously we demonstrated that a dna vaccine encoding a truncated, secreted form of bhv-1 glycoprotein b (tgb) induces cytotoxic t lymphocyte (ctl) responses in c3h mice. in this study we investigated the potential of interleukin 12 (il-12) to further enhance the ctl response. c3h mice were immunized with a plasmid encoding tgb or with plasmids encoding tgb and murine il-12. when the plasmid encoding tgb was deliv ... | 2006 | 16714071 |
| detection of bovine herpesvirus-1 infection in breeding bull semen by virus isolation and polymerase chain reaction. | serum samples from 51 apparently healthy breeding bulls were screened for bovine herpesvirus-1 (bhv-1) antibodies using an avidin-biotin enzyme-linked immunosorbent assay, revealing a sero-positive prevalence rate of 45.09%. semen samples were then collected from 12 of the sero-positive and 12 of the sero-negative bulls and tested for bhv-1 antigen using both a virus isolation assay and a polymerase chain reaction (pcr) assay; pcr was applied to detect bhv-1 deoxyribonucleic acid by using primer ... | 2005 | 16642777 |
| molecular detection of bhv-1 in artificially inoculated semen and in the semen of a latently infected bull treated with dexamethasone. | two polymerase chain reaction (pcr) assays specific for glycoprotein b (gb) and glycoprotein e (ge) gene detection, respectively, were adopted for the detection of bovine herpesvirus-1 (bhv-1) in naturally infected bulls. the methods were tested on bovine semen artificially inoculated with bhv-1 and were compared with an optimised virus isolation method. raw and extended semen samples were diluted in minimal essential medium (mem) and spiked with equal dose of bhv-1. the extended semen was found ... | 2006 | 16624722 |
| interferon-alpha genes from bos and bubalus bubalus. | interferon-a genes were cloned from six breeds of three species of two genera (three chinese native cattle breeds of yellow cattle, wild yak and huanhu domestic yak, one european breed of holstein cow, and two water buffalo breeds of fuan water buffalo and fuzhong water buffalo) by direct pcr. the pcr products were directly inserted into the expression vector to be sequenced and expressed. sequence analysis showed that ifn-a genes of six clones were composed of 498 nucleotides, encoding a mature ... | 2006 | 16621760 |
| the us9 gene of bovine herpesvirus 1 (bhv-1) effectively complements a us9-null strain of bhv-5 for anterograde transport, neurovirulence, and neuroinvasiveness in a rabbit model. | the alphaherpesvirus envelope protein us9 is a type ii viral membrane protein that is required for anterograde spread of bovine herpesvirus 5 (bhv-5) infection from the olfactory receptor neurons to the brain. in a rabbit seizure model, us9-deleted bhv-5 failed to invade the central nervous system (cns) following intranasal infection. however, when injected directly into the olfactory bulb, retrograde-spread infection from the olfactory bulb (ob) to the piriform cortex and other areas connected ... | 2006 | 16611899 |
| genetic characterisation of bovine herpesvirus 1 in new zealand. | to genotype bovine herpesvirus type 1 (bhv-1) isolates from cattle in new zealand. | 2006 | 16596156 |
| neuronal pathways of viral invasion in mice after intranasal inoculation of pseudorabies virus prv-9112c2 expressing bovine herpesvirus 1 glycoprotein b. | in contrast to wild-type pseudorabies virus (prv), which infects the central nervous system mainly via fibres of the trigeminal and autonomous nerves, the prv mutant prv-9112c2, deleted in glycoprotein b but expressing its bovine herpesvirus 1 (bhv-1) homologue, was shown to infect the swine central nervous system (cns) via the olfactory route. in this study application of prv-9112c2 into the nose of mice resulted in cns infection as described for wild-type prv. these findings indicate that gb(( ... | 2006 | 16595375 |
| stimulation of innate immune responses by cpg oligodeoxynucleotide in newborn lambs can reduce bovine herpesvirus-1 shedding. | stimulation of the innate immune system is potentially very important in neonates who have an immature adaptive immune system and vaccination cannot be used to reduce the risk of infection. cpg oligodeoxynucleotide (odn) can stimulate innate immune responses in newborn chickens and mice, but similar studies are lacking in other mammalian species. we have shown previously that cpg odn can both stimulate an acute-phase immune response and induce the antiviral effector molecule, 2'5'-a synthetase, ... | 2006 | 16584295 |
| the first immunoglobulin-like domain of porcine nectin-1 is sufficient to confer resistance to pseudorabies virus infection in transgenic mice. | nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein d (gd). porcine nectin-1 mediates entry of pseudorabies virus (prv), herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), and bovine herpesvirus type 1 (bhv-1). the gd-binding domain of nectin-1 is the first or n-terminal immunoglobulin (ig)-like domain of the entire ectodomain. here, we generated three transgenic mouse lines expressing a fusion protein consisting of the first ig-like domain of porcine nectin-1 and the f ... | 2006 | 16583156 |
| diseases and pathogens associated with mortality in ontario beef feedlots. | this study determined the prevalence of diseases and pathogens associated with mortality or severe morbidity in 72 ontario beef feedlots in calves that died or were euthanized within 60 days after arrival. routine pathologic and microbiologic investigations, as well as immunohistochemical staining for detection of bovine viral diarrhea virus (bvdv) antigen, were performed on 99 calves that died or were euthanized within 60 days after arrival. major disease conditions identified included fibrinos ... | 2006 | 16566254 |
| bhv-1 vaccine induces cross-protection against bhv-5 disease in cattle. | protection against bhv-5 disease induced by inactivated bhv-1 or bhv-5 based vaccines was analysed. two groups of calves were subcutaneously immunized with an inactivated bhv-1 or bhv-5 based vaccine. a third group was not vaccinated and used as control. in the post-vaccination period, we studied the humoral and cellular immune response resulting similar to both groups. the efficacy of the vaccines was tested after intranasal challenge of the calves with a virulent argentinean bhv-5 isolate (a-6 ... | 2006 | 16540133 |
| vaccination with a ge-negative bovine herpesvirus type 1 vaccine confers insufficient protection to a bovine herpesvirus type 5 challenge. | in the present study, cross-protection to bovine herpesvirus type 5 (bhv-5) induced by bovine herpesvirus type 1 (bhv-1) vaccination was examined following inoculation of rabbits and calves with a glycoprotein e (ge)-negative bhv-1 vaccine and subsequent challenge with bhv-5. rabbits (n=5) and calves (n=8) were vaccinated [five rabbits intranasally (in), four calves in and four intramuscularly (im)] with 7.1 log(10)median tissue culture infective dose (tcid(50)) of the bhv-1 vaccine. rabbits and ... | 2006 | 16480790 |
| ruminant alphaherpesviruses related to bovine herpesvirus 1. | herpesviruses have mainly co-evolved with their hosts for millions of years. consequently, different related host species may have been infected by various genetically related herpesviruses. illustrating this concept, several ruminant alphaherpesviruses have been shown to form a cluster of viruses closely related to bovine herpesvirus 1 (bohv-1): namely bovine herpesvirus 5, bubaline herpesvirus 1, caprine herpesvirus 1, cervid herpesviruses 1 and 2 and elk herpesvirus 1. these viruses share com ... | 2006 | 16472518 |
| herpesvirus infection in woodland caribou in alberta, canada. | sera and genital swabs collected from 121 adult woodland caribou (rangifer tarandus caribou) in five subpopulations in northern alberta, canada, between december 1997 and october 1999, were examined for evidence of infection with herpesviruses or pestiviruses. no virus was isolated from sera or swabs, and no antibodies against bovine viral diarrhea virus were detected. however, 63 (52%) of the 121 animals had neutralizing antibody titers against bovine herpesvirus 1. there was sufficient serum f ... | 2005 | 16456173 |
| the why's of y-based motifs in alphaherpesvirus envelope proteins. | the alphaherpesvirinae are large dna viruses and represent the largest subfamily of the herpesviridae with closely related members of man and animal, including herpes simplex virus, varicella-zoster virus, pseudorabies virus, bovine herpesvirus 1, and many others. the viral envelope proteins of alphaherpesviruses are remarkably diverse and are incorporated in the er, golgi, and plasma membrane of infected cells. the cytoplasmic domain of many of these envelope proteins contain specific tyrosine- ... | 2006 | 16417939 |
| nucleocytoplasmic shuttling of bovine herpesvirus 1 ul47 protein in infected cells. | previous studies with transfected cells have shown that the herpes simplex virus type 1 (hsv-1) and bovine herpesvirus 1 (bhv-1) ul47 proteins shuttle between the nucleus and the cytoplasm. hsv-1 ul47 has also been shown to bind rna. here we examine the bhv-1 ul47 protein in infected cells using a green fluorescent protein-ul47-expressing virus. we show that ul47 is detected in the nucleus early in infection. we use fluorescence loss in photobleaching to show that nuclear ul47 undergoes rapid nu ... | 2006 | 16379009 |
| intercellular trafficking of the major tegument protein vp22 of bovine herpesvirus-1 and its application to improve a dna vaccine. | intercellular spread of bovine herpesvirus-1 (bhv-1) vp22 was demonstrated in living cos-7 cells transfected with a plasmid expressing vp22-yfp (yellow fluorescence protein) and cfp (cyan fluorescence protein) bicistronically. the intercellular trafficking property of vp22 was localized to the c-terminal portion of the molecule (amino acids 121-258; vp22-c). plasmids encoding a truncated form of bhv-1 glycoprotein d (tgd) fused to vp22, vp22-c, or the n-terminal portion of vp22 (amino acids 1-12 ... | 2006 | 16374718 |
| selective apoptotic behaviour of bovine herpesvirus 1 in an epithelial-like microenvironment. | apoptosis seems to play an important role in the pathogenic profile of bovine herpesvirus 1 (bhv-1) infection. nitric oxide (no) is also important as a signal molecule. in this study, apoptosis was selectively induced in hep-2 cells in the early stage [1-3 h postinfection (pi)] of bhv-1 multiplication, and this apoptotic process was realised through the caspase-8, and partially through the caspase-3, pathway. bhv-1 infection inhibited staurosporine- (ss-) induced apoptosis only if the ss was add ... | 2005 | 16363149 |
| a homologous in vitro model to study interactions between alphaherpesviruses and trigeminal ganglion neurons. | a key aspect in the life cycle of alphaherpesviruses is their neurotropic behaviour. sensory neurons of the trigeminal ganglion (tg) are important target cells for many alphaherpesviruses (including herpes simplex virus 1, pseudorabies virus (prv), bovine herpesvirus 1) and constitute major sites for latent infections. the aim of this study was to develop an in vitro model that simulates the in vivo infection pattern of tg neurons by alphaherpesviruses. to this end, we developed a homologous in ... | 2006 | 16359830 |
| host cell targets of immediate-early protein bicp22 of bovine herpesvirus 1. | the immediate-early (ie) protein bicp22 of bovine herpesvirus 1 (bhv-1) acts as transrepressor protein on viral promoters of different kinetic classes. in the present work, we looked for host cell targets of bicp22 using a yeast two-hybrid system and identified seven candidates: (1) jik, a serine/threonine kinase of the sterile 20 protein (ste20) family that inhibits stress-related pathways; (2) camp response element binding protein-like 2 (crebl2), which in its bzip domain shares homology with ... | 2006 | 16352405 |
| functional analysis of bovine herpesvirus 1 (bhv-1) genes expressed during latency. | bovine herpes virus 1 (bhv-1) establishes latency in sensory neurons of trigeminal ganglia (tg), and germinal centers of pharyngeal tonsil. periodically bhv-1 reactivates from latency, virus is shed, and consequently virus transmission occurs. two transcripts, the latency related (lr) rna and orf-e, are abundantly expressed in tg of latently infected cattle. a lr mutant strain of bhv-1 was constructed that contains stop codons near the beginning of the lr-rna. the lr mutant virus does not expres ... | 2006 | 16352404 |
| recombination in the alphaherpesvirus bovine herpesvirus 1. | herpesviruses are dna viruses characterized by a low rate of nucleotide substitution. therefore, other mechanisms must be involved to their evolution, like recombination that can be seen as an essential evolutionary driving force of these viruses. recombination contributes to the long-term evolution of alphaherpesviruses. it acts also to continuously create new alphaherpesvirus strains. we have used bovine herpesvirus 1 to investigate recombination both within dna concatemers in infected cells a ... | 2006 | 16343820 |
| localization of sequences within the latency-related gene of bovine herpesvirus 1 that inhibit mammalian cell growth. | the latency-related (lr) rna of bovine herpes virus 1 (bhv-1) is abundantly expressed in sensory neurons of latently infected cattle. wild-type expression of lr gene products is required for the latency-reactivation cycle. lr gene products inhibit apoptosis, bicp0 expression, and mammalian cell growth. the cell growth inhibitory function of the lr gene maps to a 463-bp xbai-psti fragment. introduction of stop codons into the xbai-psti fragment had no effect on inhibiting growth. expression of a ... | 2005 | 16338750 |
| pro and contra ibr-eradication. | bovine herpesvirus type 1 (bohv-1) is the causative agent of respiratory and genital tract infections such as infectious rhinotracheitis (ibr), infectious pustular vulvovaginitis (ipv, balanoposthitis (ibp), and abortion. despite of a pronounced immune response, the virus is never eliminated from an infected host but establishes life-long latency and may be reactivated at intervals. europe has a long history of fighting against bohv-1 infections, yet, only a small number of countries has achieve ... | 2006 | 16337098 |
| a live attenuated glycoprotein e negative bovine herpesvirus 1 vaccine induces a partial cross-protection against caprine herpesvirus 1 infection in goats. | taking into account the close antigenic relationship between bovine herpesvirus 1 (bohv-1) and caprine herpesvirus 1 (cphv-1), a live attenuated glycoprotein e (ge) negative bohv-1 vaccine was assessed in goats with the aim to protect against cphv-1 infection. vaccine safety was evaluated by intranasal inoculation of two groups of goats with either a ge-negative bohv-1 vaccine or a virulent bohv-1. the length of viral excretion and the peak viral titre were reduced with the ge-negative vaccine. ... | 2006 | 16330168 |
| rationale and perspectives on the success of vaccination against bovine herpesvirus-1. | several characteristics of bhv-1 have contributed to the successful development of both conventional and marker vaccines. bhv-1 is a stable virus, which grows to high titers in vitro, has a limited host range and causes acute viremic infections. furthermore, the protective antigens, as well as the antigens that are suitable as marker, are present in the predominant virus isolates and induce significant and long-lasting immune responses, both in naïve and in previously vaccinated animals. in many ... | 2006 | 16330163 |
| bovine herpesvirus type 1 (bhv-1) up-regulates telomerase activity in mdbk cells. | the proliferative capacity of mammalian cells is regulated by telomerase, an enzyme uniquely specialised for telomeric dna synthesis. the critical role of telomerase activation in tumor progression and maintenance has been well established in studies of cancer and of oncogenic transformation in cell culture. experimental data suggest that telomerase activation has an important role in normal somatic cells, and that failure to activate sufficient telomerase also promotes disease. evidence regardi ... | 2006 | 16330162 |
| biological characterization of bovine herpesvirus 1 recombinants possessing the vaccine glycoprotein e negative phenotype. | intramolecular recombination is a frequent event during the replication cycle of bovine herpesvirus 1 (bohv-1). recombinant viruses frequently arise and survive in cattle after concomitant nasal infections with two bohv-1 mutants. the consequences of this process, related to herpesvirus evolution, have to be assessed in the context of large use of live marker vaccines based on glycoprotein e (ge) gene deletion. in natural conditions, double nasal infections by vaccine and wild-type strains are l ... | 2006 | 16321480 |
| [immunogenicity of dna vaccine expressing gp5 of porcine reproductive and respiratory syndrome virus fused with vp22 of bovine herpesvirus 1]. | to enhance the immuogenicity of dna vaccines expressing the gp5 protein of porcine reproductive and respiratory syndrome virus (prrsv), the tegument protein vp22 (encoded by vp22 gene) of bovine herpesvirus 1 (bhv-1), which has been demonstrated to exhibit the unusual protein transduction property, was fused to n-terminus of gp5 of dna vaccine construct pci-orf5m, resulting in pci-vp22-orf5m expressing vp22-gp5 fusion protein. the expression of vp22-gp5 fusion protein was confirmed by both indir ... | 2005 | 16285512 |
| pcr detection of bovine herpesviruses from nonbovine ruminants in hungary. | polymerase chain reaction (pcr) was used to test six different nonbovine ruminant species for five bovine herpesviruses including infectious bovine rhinotracheitis virus (bohv-1), bovine herpes mammillitis virus (bohv-2), movar-type herpesvirus (bohv-4), bovine herpesvirus type 5 (bohv-5), and alcelaphine herpesvirus 1 (alhv-1). species tested included 56 roe deer (capreolus capreolus), 66 red deer (cervus elaphus), 20 fallow deer (dama dama), 16 mouflon (ovis musimon), 34 domestic sheep, and 44 ... | 2005 | 16244057 |
| degenerate pcr method for identification of an antiapoptotic gene in bhv-1. | to investigate on the hypothetical presence of an antiapoptotic gene, we utilized the codehop (consensus-degenerate hybrid oligonucleotide primers) strategy amplifying unknown sequences from a background of genomic (bovine herpesvirus type-1) bhv-1 dna. an alignment of carboxyl-terminal domains belonging to three proteins encoded by gamma34.5, myd116 and gadd34 genes, was carried out to design degenerate pcr primers in highly conserved regions. this allowed the amplification of a 110 bp fragment ... | 2006 | 16237705 |
| quantification of the transmission of bovine herpesvirus 1 among red deer (cervus elaphus) under experimental conditions. | bovine herpesvirus 1 (bhv1) is endemically present in a cattle population that lives in a nature reserve in the netherlands. red deer (cervus elaphus), living in the same nature reserve, can come into contact with the bhv1-infected cattle and could then become infected with bhv1. for the eradication of bhv1 in cattle, it is, therefore, important to know whether red deer alone can play a role in the transmission of bhv1. for that reason, we quantified the transmission of bhv1 among farmed red dee ... | 2005 | 16226408 |
| [experiences with the eradication of the bovine herpes virus 1 in lower saxony]. | in this paper first the general cascade of animal disease eradication from the voluntary control programme up to culling of infected animals is described. afterwards the development of the eradication of bovine herpes virus in lower saxony is illustrated. obvious the number of farms which are not eradicating decreases since the testing on bhv1 is obligate in germany. in 2004 60% of the cattle holdings were free from bhv1. at least the measures which will be taken in lower saxony to increase the ... | 2005 | 16218185 |
| the bovine herpesvirus 1 gene encoding infected cell protein 0 (bicp0) can inhibit interferon-dependent transcription in the absence of other viral genes. | the infected cell protein 0 (bicp0) encoded by bovine herpesvirus 1 (bhv-1) stimulates viral gene expression and productive infection. as bicp0 is expressed constitutively during productive infection, it is considered to be the major viral regulatory protein. like other alphaherpesvirus icp0 homologues, bicp0 contains a zinc ring finger near its n terminus that activates transcription and regulates subcellular localization. in this study, evidence is provided that bicp0 represses the human beta ... | 2005 | 16186222 |
| high-level expression of biologically active bovine alpha interferon by bovine herpesvirus 1 interferes only marginally with recombinant virus replication in vitro. | an artificial open reading frame (orf) for bovine alpha interferon (boifn-alpha) with the codon preference of bovine herpesvirus 1 (bhv-1) glycoprotein b was constructed to assess the effect of expression of boifn-alpha by bhv-1 from an expression cassette. transient expression of the orf revealed that transfected cells secreted substantial amounts of biologically active boifn-alpha, which moderately inhibited replication of bhv-1 after stimulation of bovine cells with 10(4) u ml(-1). the boifn- ... | 2005 | 16186221 |
| the physiological and production effects of increased dietary intake of vitamins e and c in feedlot cattle challenged with bovine herpesvirus 1. | the physiological and production effects of feeding additional vitamin e and ruminally protected vitamin c were examined in cattle challenged with bovine herpesvirus 1 (bhv 1). forty-eight individually penned 6-mo-old angus and angus crossbred heifer calves with a mean bw of 151 kg were allocated randomly to four diets in a 2 x 2 factorial arrangement of treatments. pelleted diets provided either 15 or 185 iu/kg of dm of vitamin e, with or without 3.7 g of ruminally protected vitamin c/kg of dm. ... | 2005 | 16160055 |
| immunization with plasmid dna encoding a truncated, secreted form of the bovine viral diarrhea virus e2 protein elicits strong humoral and cellular immune responses. | the major protective antigen of bovine viral diarrhea virus (bvdv), the e2 protein, is cell-associated and not expressed on the cell surface. in this study we evaluated a dna vaccine encoding various secreted versions of e2. in vitro analysis demonstrated that deletion of the transmembrane anchor and addition of the signal sequence of bovine herpesvirus-1 (bhv-1) (gdsdeltae2) resulted in efficient secretion of e2 into the culture medium. in contrast, full-length e2, either without or with gds (g ... | 2005 | 16154245 |
| effect of different nuclear localization sequences on the immune responses induced by a midge vector encoding bovine herpesvirus-1 glycoprotein d. | to optimize the efficiency of a minimalistic immunogenically defined gene expression (midge) vector, peptides containing proven (sv40 t-antigen and bovine herpesvirus-1 vp8) or putative (herpes simplex virus-1 vp22) nuclear localization signals (nlss) were linked to a midge vector encoding a truncated, secreted form of bhv-1 glycoprotein d (tgd) (midge-tgd). conjugation of an nls to the midge-tgd vector improved the tgd expression in vitro and the humoral and cellular immune responses induced in ... | 2006 | 16154243 |
| the amino-terminal residue of glycoprotein b is critical for neutralization of bovine herpesvirus 1. | in order to address the neutralization epitope on bovine herpesvirus 1 (bhv1) glycoprotein b (gb), a panel of monoclonal antibodies (mabs), a series of truncation forms of the glycoprotein and an mab-escape mutant were used in this study. immunocytochemistry on the truncations using mabs against the glycoprotein revealed that the neutralization epitopes recognized by the mabs lay between residues 1 and 52 of mature gb. comparison of the sequences among the mutant, parent, and revertant viruses d ... | 2006 | 16153736 |
| characterization of the nuclear localization and nuclear export signals of bovine herpesvirus 1 vp22. | the bovine herpesvirus 1 (bhv-1) tegument protein vp22 is predominantly localized in the nucleus after viral infection. to analyze subcellular localization in the absence of other viral proteins, a plasmid expressing bhv-1 vp22 fused to enhanced yellow fluorescent protein (eyfp) was constructed. the transient expression of vp22 fused to eyfp in cos-7 cells confirmed the predominant nuclear localization of vp22. analysis of the amino acid sequence of vp22 revealed that it does not have a classica ... | 2005 | 16140763 |
| characterization of caprine herpesvirus 1 glycoprotein d gene and its translation product. | caprine herpesvirus 1 (cphv-1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. this virus is closely related to bovine herpesvirus 1 (bohv-1) which causes infectious bovine rhinotracheitis. glycoprotein d (gd) mediates important functions in alphaherpesviruses and is also a main immunogen. the sequence of cphv-1 gd gene and the biochemical properties of its translation product were analyzed and compared to those of bohv-1 and othe ... | 2006 | 16140410 |
| detection of equine herpesvirus type 1 using a real-time polymerase chain reaction. | equid herpesvirus 1 (ehv1) is a major disease of equids worldwide causing considerable losses to the horse industry. a variety of techniques, including pcr have been used to diagnose ehv1. some of these pcrs were used in combination with other techniques such as restriction enzyme analysis (rea) or hybridisation, making them cumbersome for routine diagnostic testing and increasing the chances of cross-contamination. furthermore, they involve the use of suspected carcinogens such as ethidium brom ... | 2006 | 16137772 |
| phylogeny and antigenic relationships of three cervid herpesviruses. | elk herpesvirus (elkhv) from north american elk (wapiti, cervus elaphus nelsoni) is a recently identified alphaherpesvirus related to bovine herpesvirus-1 (bhv-1). in this study, we determined its relationship with european cervid herpesviruses: cervid herpesvirus-1 (cerhv-1) from red deer and rangiferine herpesvirus (ranhv) from reindeer. for phylogenetic analysis, genes for the gc and gd proteins of these viruses were sequenced. these genes demonstrated an extremely high gc content (76-79%). g ... | 2005 | 16061301 |
| inhibition of bovine sperm-zona binding by bovine herpesvirus-1. | the purpose of the present study was to identify a potential interference of bovine herpesvirus-1 (bohv-1) with sperm-oocyte interactions during bovine in vitro fertilization. an inhibition of almost 70% of sperm-zona binding was observed when bovine cumulus-denuded oocytes were inseminated in the presence of 10(7) 50% tissue culture infective dose/ml bohv-1. the inhibitory effect of bohv-1 on sperm-zona binding was mediated by an interaction of the virus with spermatozoa, but not with oocytes. ... | 2005 | 16049163 |
| a monoclonal antibody-based elisa allows discrimination between responses induced by bovine herpesvirus subtypes 1 (bohv-1.1) and 2 (bohv-1.2). | bovine herpesvirus type 1 (bohv-1) has distinct subtypes according to genomic characterization. immune responses induced by bohv-1 subtype 1 (bohv-1.1) are not distinguishable from those induced by bohv-1 subtype 2 (bohv-1.2) through conventional serological methods. in the present report, an enzyme linked immunosorbent assay is described that allows discrimination between immune responses in cattle immunized with either subtype, based on a monoclonal antibody that recognizes specifically the am ... | 2005 | 16046004 |
| isolation and characterisation of cervine herpesvirus-1 from red deer semen. | this communication describes the isolation of herpesvirus during routine export examination of semen collected from red deer stags in new zealand. | 2001 | 16032173 |
| evaluation of an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of infectious bovine rhinotracheitis infection, with results of a preliminary survey. | an indirect enzyme linked immunosorbent assay (elisa) procedure was evaluated against the serum neutralisation test (snt) for the detection of antibodies to infectious bovine rhinotracheitis virus (bovine herpesvirus type l), using 2028 sera from 166 dairy and 172 beef cattle herds. the results showed the elisa to give high levels of agreement with the snt in classifying positive and negative sera (98% and 97% respectively). such disagreements as did occur involved weakly reactive sera with snt ... | 1986 | 16031249 |
| an outbreak of vulvovaginitis in goats caused by a caprine herpesvirus. | a caprine herpesvirus related to infectious bovine rhinotracheitis virus but immunologically distinct from that virus was isolated from an outbreak of vulvovaginitis in a herd of saanen goats. the morbidity rate was 52.5%, with 21 of 40 does showing clinical signs. the lesions healed rapidly with only two goats showing lesions two weeks after the disease was first detected. no effect on subsequent reproductive performance was observed. the mode of transmission of the virus was believed to be ven ... | 1982 | 16030825 |
| prime-boost strategies combining dna and inactivated vaccines confer high immunity and protection in cattle against bovine herpesvirus-1. | dna vaccines have frequently been associated with poor efficacy in large animals. in the present study, one administration of an inactivated marker vaccine to cattle considerably boosted both humoral and cellular arms of the immune response primed with bovine herpesvirus-1 (bohv-1) dna vaccines encoding glycoprotein d (gd) or gc+gd. calves vaccinated according to the dna prime-inactivated boost also showed significantly enhanced virological protection as compared to controls. the 4-logarithms re ... | 2005 | 16024138 |
| longevity of protective immunity to experimental bovine herpesvirus-1 infection following inoculation with a combination modified-live virus vaccine in beef calves. | to determine whether a combination viral vaccine containing modified-live bovine herpesvirus-1 (bhv-1) would protect calves from infection with a recent field isolate of bhv-1. | 2005 | 16013547 |
| evaluation of risks of viral transmission to recipients of bovine embryos arising from fertilisation with virus-infected semen. | this scientific review was prompted by recent legislation to curtail the use of semen from potentially virus-infected bulls to produce embryos for import into the european union. from studies in laboratory animals, humans and horses, it is apparent that viruses may sometimes attach to, or be integrated into, spermatozoa, although in domestic livestock, including cattle, this seems to be a rare phenomenon, and carriage of virus through the zona pellucida into the oocyte by fertilising sperm has n ... | 2006 | 16005506 |
| complexities of the pathogenesis of mannheimia haemolytica and haemophilus somnus infections: challenges and potential opportunities for prevention? | progress in producing improved vaccines against bacterial diseases of cattle is limited by an incomplete understanding of the pathogenesis of these agents. our group has been involved in investigations of two members of the family pasteurellaceae, mannheimia haemolytica and haemophilus somnus, which illustrate some of the complexities that must be confronted. susceptibility to m. haemolytica is greatly increased during active viral respiratory infection, resulting in rapid onset of a severe and ... | 2004 | 15984339 |
| analysis of a bovine herpesvirus 1 recombinant virus that does not express the bicp0 protein. | bovine herpesvirus 1 (bhv-1) infected-cell protein 0 (bicp0) stimulates productive infection by activating viral gene expression. in this study, an attempt was made to construct a recombinant virus with point mutations in the c(3)hc(4) zinc ring finger of bicp0, as this domain is necessary for activating viral transcription and productive infection. a virus was identified in bovine cells that induced small clusters of infected cells resembling a small plaque. instead of the expected mutations wi ... | 2005 | 15958678 |
| genetic immunisation of cattle against bovine herpesvirus 1: glycoprotein gd confers higher protection than glycoprotein gc or tegument protein vp8. | bovine herpesvirus 1 (bohv-1) has frequently been used as a model for testing parameters affecting dna immunisation in large animals like cattle. however, the selection of target antigens has been poorly studied, and most of the experiments have been conducted in mice. in the present study, we demonstrated in cattle that a dna vaccine encoding bohv-1 glycoprotein gd induces higher neutralising antibody titres than vaccines encoding bohv-1 gc. additionally, we show that a dna vaccine encoding a s ... | 2005 | 15955279 |
| relative efficacy of inactivated bovine herpesvirus-1 (bhv-1) vaccines. | the relative efficacy of four commercially available inactivated bovine herpesvirus-1 (bhv-1) vaccines was directly assessed in controlled studies in 5-6-month-old, bhv-1 naïve calves. the sero-response due to the basic course of two intramuscular vaccinations was closely similar for three of four vaccines whereas one vaccine did not induce seroconversion in five of six calves. at the level of challenge virus shedding in nasal mucus, all four vaccines were significantly protective but to a marke ... | 2005 | 15951068 |
| rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein c gene in clinical specimens by multiplex-pcr. | a multiplex polymerase chain reaction (multiplex-pcr) to detect and differentiate bovine herpesvirus 1 (bohv-1) and 5 (bohv-5) was developed using primers for the gene sequence that encodes the glycoprotein c. the technique was assessed against the bohv-1 and bohv-5 cell culture adapted strains, and clinical samples collected from animals with clinical signs of bohv-1 (n = 10) or bohv-5 (n = 7) infection and with diagnosis confirmed by virus isolation in cell culture and semi-nested pcr. fifteen ... | 2005 | 15939490 |
| modification of active immunization with live bovine herpesvirus 1 vaccine by passive viral antibody. | the study in bhv-1 naïve calves investigated the effect of intramuscularly (im) administered bhv-1 neutralising bovine immunoglobulin on the efficacy of a live intranasally (in) administered bhv-1 vaccine. overall on daily basis there was between 40- and 5000-fold less vaccine virus shed by the passively immune calves compared with that shed by the naïve counterparts. the latter seroconverted to the vaccine whereas the virus neutralising (vn) antibody titres in the passively immune calves decrea ... | 2005 | 15916839 |
| risk factors for bovine herpesvirus-1 seropositivity. | this paper reports the investigation of risk factors for bovine herpesvirus-1-seropositivity, based on a cluster-sample survey of the belgian cattle population. this serosurvey was carried out in 1998 in 309 randomly selected unvaccinated herds of all types (dairy, mixed and beef) were all bovids (n = 11,284) were sampled. older and male cattle had higher seroprevalence. origin (homebred or purchased) and herd size interacted; for smaller herds (< or = 50 cattle on the premises), purchase status ... | 2005 | 15907575 |
| bovine herpesvirus type 1 abortions detected by a semi-nested pcr in brazilian cattle herds. | bovine herpesvirus type 1 (bhv-1) was investigated by a semi-nested polymerase chain reaction (sn-pcr) and by mdbk cell culture virus isolation in organ fragments from 55 aborted fetuses collected from beef and dairy cattle herds with history of reproductive problems in the north of paraná state, brazil. a 425 bp amplicon of the bhv-1 glycoprotein d gene was detected in 14 (25.4%) aborted fetuses. bhv-1 was isolated in mdbk cells from the tissue of 5 (9.1%) fetuses. the specificity of positive r ... | 2004 | 15894030 |
| serological status for n. caninum, bovine viral diarrhea virus, and infectious bovine rhinotracheitis virus at pregnancy testing and reproductive performance in beef herds. | neospora caninum, infectious bovine rhinotracheitis (ibr), and bovine viral diarrhea virus (bvdv) are important differentials for the diagnosis of infectious reproductive loss in beef herds. the objective of this study was to describe the serological status of both pregnant and non-pregnant beef cows from herds with varying levels of reproductive success. the study provided an opportunity to examine whether there were any associations between serological status for bvdv, ibr, and n. caninum and ... | 2005 | 15893892 |
| seroprevalence of infectious bovine rhinotracheitis in mithun (bos frontalis) in india. | a preliminary study on the seroprevalence of infectious bovine rhinotracheitis (ibr) in different strains of mithun maintained atthe national research centre on mithun, nagaland, india, revealed that the overall prevalence of ibr in these mithun was 19%. this paper examines the results of that preliminary study. to the knowledge of the authors, this is the first report on the seroprevalence of ibr in mithun in india. the highest prevalence of ibr was observed in mithun found in arunachal pradesh ... | 2004 | 15861877 |
| enhancement of antigen acquisition by dendritic cells and mhc class ii-restricted epitope presentation to cd4+ t cells using vp22 dna vaccine vectors that promote intercellular spreading following initial transfection. | induction of immune responses against microbial antigens using dna is an attractive strategy to mimic the immunity induced by live vaccines. although dna vaccines are efficacious in murine models, the requirement for multiple immunizations using high doses in outbred animals and humans has hindered deployment. this requirement is, in part, a result of poor vaccine spreading and suboptimal dc transfection efficiency. incorporation of a signal that directs intercellular spreading of a dna-encoded ... | 2005 | 15857936 |
| characteristics of live bovine herpesvirus-1 vaccines. | the common disease caused by bovine herpes virus 1 infection is febrile rhinotracheitis (frt) and under certain conditions the virus is strongly implicated in pre-disposing cattle to pneumonic pasteurellosis. these illnesses account for a significant economic loss in the cattle industry worldwide and vaccination is widely applied. in naive cattle, and for a short period of time, old and new vaccines significantly reduce clinical signs of frt and also virus shedding. a single intranasal vaccinati ... | 2005 | 15848783 |
| experimental exposure of pigs to infectious bovine rhinotracheitis (ibr) virus. | thirteen specific-pathogen-free pigs were used in an experiment to determine the pathogenicity of ibr virus for pigs. three pigs were used as controls, eight were exposed to ibr virus by either the intravenous, subcutaneous, intratracheal, or intranasal route, and two pigs served as contact controls. the clinical response consisted of a temperature rise, depression and variable lymphocytosis. the major tissue alterations were interstitial pneumonitis, and peribronchiolitis with round cell aggreg ... | 1968 | 15846897 |
| evaluation of promoters for foreign gene expression in the e3 region of bovine adenovirus type-3. | in order to optimize foreign gene expression in the e3 region of badv-3, we constructed full-length badv-3 genomic dna clones containing a reporter gene (truncated glycoprotein gd of bovine herpesvirus 1, gdt), under the control of exogenous promoters inserted in either direction in the e3 region. irrespective of exogenous transcriptional elements, viable recombinant badv-3 viruses could only be isolated when the gdt expression cassettes were inserted in the e3 region parallel to the direction o ... | 2005 | 15845268 |
| non-transmission of bacterial and viral microbes to embryos and semen stored in the vapour phase of liquid nitrogen in dry shippers. | cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (vpln) to embryos and semen in dewars designed for short-term storage and transportation of biological specimens. in this study transmission of pseudomonas aeruginosa, escherichia coli, staphylococcus ... | 2005 | 15843010 |
| serologic surveillance for selected viral agents in captive and free-ranging populations of arabian oryx (oryx leucoryx) from saudi arabia and the united arab emirates. | a total of 294 sera collected between 1999 and 2001 from eight captive and one free-ranging herds of arabian oryx (oryx leucoryx) distributed in saudi arabia (sa) and the united arab emirates (uae) were assayed for antibodies against 13 selected viral agents. arabian oryx have been exposed to bluetongue virus (btv), epizootic hemorrhagic disease virus (ehdv), rinderpest virus (rpv), bovine respiratory syncytial virus (brsv), bovine adenovirus 3 (bav-3), cervid herpesvirus-1, foot-and-mouth disea ... | 2005 | 15827212 |
| experimental microbial contamination and disinfection of dry (vapour) shipper dewars designed for short-term storage and transportation of cryopreserved germplasm and other biological specimens. | cryopreservation, storage and transport of cryopreserved germplasm without the risk of disease transmission is of great concern to animal and human health authorities. here we report on the efficacy of microbial decontamination of the liquid nitrogen (ln) dry (vapour) shippers used for short-term storage and transportation of germplasm and other biological specimens. dry shippers containing either a hydrophobic or a non-hydrophobic ln absorbent were experimentally contaminated with high titers o ... | 2005 | 15823351 |
| varicelloviruses avoid t cell recognition by ul49.5-mediated inactivation of the transporter associated with antigen processing. | detection and elimination of virus-infected cells by cytotoxic t lymphocytes depends on recognition of virus-derived peptides presented by mhc class i molecules. a critical step in this process is the translocation of peptides from the cytoplasm into the endoplasmic reticulum by the transporter associated with antigen processing (tap). here, we identified the bovine herpesvirus 1-encoded ul49.5 protein as a potent inhibitor of tap. the expression of ul49.5 results in down-regulation of mhc class ... | 2005 | 15793001 |
| immunization with a bovine herpesvirus 1 glycoprotein b dna vaccine induces cytotoxic t-lymphocyte responses in mice and cattle. | virus-specific cytotoxic t lymphocytes (ctls) are considered to be important in protection against and recovery from viral infections. in this study, several approaches to induce cytotoxicity against bovine herpesvirus 1 (bhv-1) were evaluated. vaccination of c57bl/6 mice with bhv-1 induced a strong humoral, but no ctl, response, which may be due to downregulation of major histocompatibility complex class i molecules. in contrast, vaccinia virus expressing glycoprotein b (gb) elicited a weaker a ... | 2005 | 15784883 |
| identification of functional domains within the bicp0 protein encoded by bovine herpesvirus 1. | it is believed that the bicp0 protein encoded by bovine herpesvirus 1 (bohv-1) stimulates productive infection by activating viral gene expression. like the other icp0-like proteins encoded by alphaherpesvirinae subfamily members, bicp0 contains a zinc ring finger near its amino terminus. the zinc ring finger of bicp0 activates viral transcription, stimulates productive infection, and is toxic to certain cell types. apart from the zinc ring finger, bicp0 possesses little similarity to the herpes ... | 2005 | 15784882 |
| prolonged persistence of bovine herpesvirus in small cattle herds: a model-based analysis. | herpesviruses can remain dormant in once-infected hosts and, upon reactivation, cause such hosts to become infectious. this phenomenon of latency and reactivation may enable herpesviruses to persist for a long time in small host populations. to quantify the effect of reactivation on persistence, the time to extinction of bovine herpesvirus type 1 (bhv-1) in small cattle populations was calculated. for realistic parameter values the mean time to extinction is already more than 100 years in a popu ... | 2005 | 15724721 |
| nuclear and mitochondrial localization signals overlap within bovine herpesvirus 1 tegument protein vp22. | vp22, a tegument protein of bovine herpesvirus 1, accumulates in the nucleus of infected and transiently transfected cells. previous studies indicated a possible regulatory function of vp22 within nuclei, but how vp22 enters nuclei is unknown. despite the abundance of basic residues within this protein, no classic nuclear localization signal (nls) motif has been identified. to identify the signal directing nuclear accumulation, a series of truncations, internal deletions, and point mutations wer ... | 2005 | 15705574 |
| bovine herpesvirus vp22 induces apoptosis in neuroblastoma cells by upregulating the expression ratio of bax to bcl-2. | herpesvirus tegument protein vp22 has been shown to have biotherapeutic potential in tumor gene therapy. some studies indicate that vp22 may enhance the transfer efficiency of therapeutic proteins by delivering them to more cells while trafficking. our previous study showed that bovine herpesvirus vp22 (bvp22) enhanced equine herpesvirus thymidine kinase-ganciclovir (etk-gcv) suicide gene therapy by an unknown intracellular effect. in this study, the interaction between bvp22 and host tumor cell ... | 2005 | 15703493 |
| synthesis, in vitro cytotoxic and antiviral activity of cis-[pt(r(-) and s(+)-2-alpha-hydroxybenzylbenzimidazole)2cl2] complexes. | a pair of enantiomeric platinum(ii) complexes of cis-[pt(r(-) and s(+)-hbb)2cl2] (hbb=2-alpha-hydroxybenzylbenzimidazole) was synthesized and evaluated for its preliminary in vitro cytotoxic activity on the human mcf-7 breast cancer and hela cervix cancer cell lines and antiherpes virus activity against bovine herpesvirus type 1 (bhv-1). in general, it was found that pt(ii) complexes were less cytotoxic on both cell lines than cisplatin and were comparable to carboplatin. there was no significan ... | 2005 | 15694648 |
| formulation with cpg oligodeoxynucleotides prevents induction of pulmonary immunopathology following priming with formalin-inactivated or commercial killed bovine respiratory syncytial virus vaccine. | commercial killed bovine respiratory syncytial virus (k-brsv) and formalin-inactivated brsv (fi-brsv) tend to induce th2-type immune responses, which may not be protective and may even be detrimental during subsequent exposure to the virus. in this study we assessed the ability of cpg oligodeoxynucleotides (odns) to aid in the generation of effective and protective brsv-specific immune responses. mice were immunized subcutaneously with fi-brsv formulated with cpg odn, emulsigen (em), cpg odn and ... | 2005 | 15681404 |
| observations on bovine herpesvirus type 1 infection in cattle in the uk. | 2005 | 15675530 | |
| bvdv and bhv-1 infections in dairy herds in northern and northeastern thailand. | bulk milk samples from 220 dairy herds were collected at 9 public milk collection centres in the northeastern and northern thailand, and a subset of 11 herds was selected for individual testing. the samples were tested for presence of antibodies to bvdv and bhv-1 using an indirect elisa. the results from the bulk milk testing demonstrated a moderate level of exposure to bvdv and bhv-1 (73% and 67%, respectively). however, the low proportion of herds with high bvdv antibody-levels (13%) and the l ... | 2004 | 15663078 |
| bovine herpesvirus 1 vp22 enhances the efficacy of a dna vaccine in cattle. | for this study, the intercellular trafficking ability of bovine herpesvirus 1 (bhv-1) vp22 was applied to improve the efficacy of a dna vaccine in calves. a plasmid encoding a truncated version of glycoprotein d (tgd) fused to vp22 was constructed. the plasmid encoding tgd-vp22 elicited significantly enhanced and more balanced immune responses than those induced by a plasmid encoding tgd. furthermore, protection against a bhv-1 challenge was obtained in calves immunized with the plasmid encoding ... | 2005 | 15650221 |
| latency-related gene encoded by bovine herpesvirus 1 promotes virus growth and reactivation from latency in tonsils of infected calves. | infection of calves with bovine herpesvirus 1 (bhv-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. although sensory neurons in the trigeminal ganglia (tg) are the primary site of bhv-1 latency, viral genomes are detected in the tonsils of latently infected calves. dexamethasone (dex) consistently induces reactivation from latency, and viral gene expression is detected in tg and tonsils. in sensory neurons of latently infected calve ... | 2005 | 15635000 |