Publications

[helicobacter pylori: studies in domestic swine].	in the course of 15 month, 240 stomachs from pigs slaughtered in three different slaughterhouses were examined for the presence of helicobacter pylori. in 52.5% of the stomachs, gram-negative curved or spiral shaped bacteria were found, which during the following biochemical analyses were demonstrated to be urease negative. helicobacter pylori could not be isolated from any of the stomachs. in addition, we conducted four separate experiments on five to eight weeks old piglets in order to determi ...	1993	8306861
high-dose epinephrine results in greater early mortality after resuscitation from prolonged cardiac arrest in pigs: a prospective, randomized study.	to determine whether high-dose epinephrine (0.2 mg/kg) during cardiopulmonary resuscitation (cpr) results in improved outcome, compared with standard-dose epinephrine (0.02 mg/kg).	1994	8306688
myocardial glucose transporters and glycolytic metabolism during ischemia in hyperglycemic diabetic swine.	we assessed the effects of 4 weeks of streptozocin-induced diabetes on regional myocardial glycolytic metabolism during ischemia in anesthetized open-chest domestic swine. diabetic animals were hyperglycemic (12.0 +/- 2.1 v 6.6 +/- .5 mmol/l), and had lower fasting insulin levels (27 +/- 8 v 79 +/- 19 pmol/l). myocardial glycolytic metabolism was studied with coronary flow controlled by an extracorporeal perfusion circuit. left anterior descending coronary artery (lad) flow was decreased by 50% ...	1994	8289677
mustard oil-induced cutaneous inflammation in the pig.	recent findings indicate that chemical stimulation of the porcine skin with capsaicin evokes a flare response similar to that observed in man. the aim of the present study was to elucidate whether chemical stimulation of cutaneous capsaicin-sensitive nerve endings with mustard oil produces neurogenic inflammatory reactions in the pig. the application of mustard oil onto the abdominal skin of domestic pigs resulted in a pronounced flare response. after a previous intravenous injection of a soluti ...	1993	8285137
retroperitoneal endoscopic adrenalectomy: an experimental study.	laparoscopic approaches to adrenalectomy have been limited by the retroperitoneal (rp) location of the adrenal glands and their relative inaccessibility transabdominally. we developed a technique for endoscopic adrenalectomy in a domestic swine model using insufflation of the rp space with co2 and retroperitoneoscopy. the technique for retroperitoneal endoscopic adrenalectomy was first developed in an acute study of three animals. a chronic survival study was then undertaken in six pigs. unilate ...	1993	8269248
international trade in bear gall bladders: forensic source inference.	fresh and desiccated gall bladders of the ursidae family (bears) obtained as criminal evidence were characterized by analysis of the principal biliary components, mainly ursodeoxycholyl-taurine, cholyl-taurine and chenodeoxycholyl-taurine using tlc and hplc. this bile acids profile appears to be an ursidae family characteristic. results show that of the samples from asia only 3% were from the ursidae family and 18% were from "farmed bears." samples seized in the u.s.a. and canada showed that 22. ...	1993	8263479
laparoscopic small bowel resection and anastomosis.	our aim was to assess the feasibility and safety of performing totally intracorporeal laparoscopic small bowel resection and anastomosis using a linear stapling device. laparoscopic small bowel resection and anastomosis were performed in five young domestic pigs. five trocars were used (2-5 mm, 2-11 mm, and 1-12 mm) for video laparoscopic access to the peritoneal cavity. segments of jejunum 5 to 10 cm long were excised using two firings of a 30-mm linear stapler (endo-gia). the bowel was anastom ...	1993	8258075
vasoactivity of isolated coronary arterial microvessels: influence of albumin.	previous investigators have reported the development of spontaneous tone, a spontaneous decrease in diameter in response to application of intraluminal pressure, in isolated microvessels bathed in physiological salt solution (pss) containing albumin. in contrast, myogenic activity and spontaneous tone are rare in microvessels bathed in albumin-free pss. the purpose of this study was to determine whether albumin and/or the development of spontaneous tone altered coronary microvessel responsivenes ...	1993	8246812
the sex-determining region y (sry) gene is mapped to p12-p13 of the y chromosome in pig (sus scrofa domestica) by in situ hybridization.	the sex-determining region y is a gene located in the distal portion of the short arm of human (sry) and mouse (sry) y chromosomes and considered to be the best candidate for the testis determining factor (tdf/tdy). the gene is believed to be the key factor in sex differentiation in mammals and is conserved across mammalian species. we report herein that the sry/sry gene has been assigned to p12-p13 on the short arm of the y chromosome in pig by in situ hybridization. the result confirms intersp ...	1993	8239073
folate sensitive common fragile sites in chromosomes of the domestic pig (sus scrofa).	chromosomal fragile sites were studied in blood lymphocytes of the pig after induction with 0, 5, 10 and 20 micrograms of fluorodeoxyuridine (fudr) ml-1 of culture. the percentage of cells showing fragile sites and total breaks and gaps in 100 cells was significantly higher in all treated cultures than the control (p < 0.001). a concentration of 5 to 10 micrograms of fudr ml-1 of medium is recommended as the optimum for mitotic index and fragile site expression in the pig. localisation of the fr ...	1993	8235092
dobutamine prevents both myocardial stunning and phosphocreatine overshoot without affecting atp level.	catecholamines can overcome myocardial stunning. however, a previous report on energy metabolism in stunned myocardium during catecholamine infusion was based on the conventional biochemical methods which might affect contractile function. twenty farm pigs were anesthetized and underwent 15 min coronary artery occlusion and 2 h reperfusion. ten pigs were given 10 micrograms/kg/min dobutamine from immediately after and throughout the reperfusion (dobutamine group). the other ten pigs were given s ...	1993	8230247
antagonism of antidiuretic hormone in domestic pigs (sus scrofa).	1. morphological and physiological aspects of renal function are shared by humans and swine. sk&f 101926 is a potent antagonist of vasopressin binding to v2 receptors and vasopressin stimulation of adenylate cyclase activity in renomedullary membranes from both species. 2. unexpectedly, sk&f 101926 proved to be an antidiuretic agonist in humans. hence, we evaluated sk&f 101926 for antidiuretic agonist and antagonist activities in conscious domestic pigs. 3. during water diuresis (uosm < 230 mosm ...	1993	8224729
gene sequence, cdna construction, expression in escherichia coli and genetically approached purification of porcine interleukin-1 beta.	a genomic clone (pil3) covering the 8.8-kb prointerleukin-1 beta ('catabolin') gene of the domesticated swine (sus scrofa domestica) was isolated from a genomic library and characterized by nucleotide sequencing. typical features of the gene include a seven-exon structure, with the highest degree of nucleotide and amino acid conservation among human and porcine genes being found in the receptor-binding portion encoded by exons six and seven. three 250-bp repetitive elements with a > 75% similari ...	1993	8223584
application of a blocking enzyme-linked immunosorbent assay for serological monitoring of hog cholera (classical swine fever) in poland.	between 1990 and 1992, serum samples from 55,478 domestic swine were tested by enzyme-linked immunosorbent assay (elisa) for the presence of hog cholera virus (hcv) antibodies. the amount of antibody in the sera was expressed as the mean percentage inhibition (pi). for diagnosis, the tested sera were diluted 1:2 and considered positive if the pi was less than 25%. sera giving pi values in the range of 25-50% were retested against hcv and bovine virus diarrhoea virus (bvdv), by neutralising perox ...	1993	8219338
identification and cytogenetic analysis of an abnormal pig chromosome for flow cytometry and sorting.	for cytogenetics of pig (sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to establish dna-libraries, or to produce dna-probes. flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. flow sorting of the largest chromosomes of these cells was performed. after sorting the chrom ...	1993	8216615
nimodipine after circulatory arrest: effects on oxygen delivery and consumption.	evaluation of the effects of nimodipine administration during and after cardiopulmonary resuscitation (cpr) on oxygen delivery and consumption was the aim of this study.	1994	8199650
an animal model to study the significance of dermis for grafting cultured keratinocytes on full thickness wounds.	autologous cultured keratinocytes grafted onto full thickness wounds take poorly, and any epidermal cover that is produced is unstable. however, a more stable epidermis has been reported when keratinocytes are grafted onto a dermal surface. we have developed a skin grafting model using a polytetrafluoroethylene (ptfe) skin graft chamber in the domestic pig. the chambers isolate individual wounds and prevent epithelial migration from the wound edge. dermal grafts were prepared by enzymatic separa ...	1994	8198746
additive effect of allopurinol and deferoxamine in the prevention of spinal cord injury caused by aortic crossclamping.	fourteen domestic swine were divided into two groups. group a (n = 7) was the control group, in which no pharmacologic intervention was applied. in group b (n = 7), the ischemic-reperfused spinal cord was treated with the combination of allopurinol (50 mg/kg/day for 3 days before the day of operation) and deferoxamine (desferal, 50 mg/kg administered intravenously over 3 to 4 hours). the administration of deferoxamine was completed 1 hour before crossclamping. the crossclamp was placed on the de ...	1994	8176962
refinement of a technique for thermocholecystectomy in an animal model.	a modification of a thermal ablation system was tested for improved cystic duct occlusion and gallbladder mucosa ablation in an animal model.	1994	8175312
[fetometry in domesticated swine].		1993	8172779
epidemiology of classical swine fever in sardinia: a serological survey of wild boar and comparison with african swine fever.	a serological survey was carried out to establish the distribution of classical swine fever among wild boar in sardinia, where that disease and african swine fever have been endemic in free-ranging domestic pigs and wild boar living in the mountainous areas of the province of nuoro for several years. blood samples were collected from 4752 wild boar shot during the period december 1988 to january 1992. an overall prevalence of 11 per cent was observed and the almost constant rate of about 9.8 per ...	1994	8171792
screening of pig sera for antibodies to japanese encephalitis virus using a dot enzyme immunoassay and igm capture elisa: comparison with the hemagglutination inhibition and plaque reduction neutralization tests.	a dot enzyme immunoassay for determination of antibodies to japanese encephalitis virus was designed for use as a field technique for the surveillance of japanese encephalitis virus activity among domestic pigs. the test was compared with the neutralization test and the hemagglutination inhibition test and found to be more sensitive than the hemagglutination inhibition test and comparable to the neutralization test in sensitivity but more simple to perform than either the neutralization or the h ...	1993	8160055
laparoscopic distal pancreatectomy in the porcine model.	our aim was to assess the feasibility and safety of laparoscopic distal pancreatectomy in an animate model. after developing the technique in acute animal experiments, laparoscopic distal pancreatectomy was performed in five young domestic pigs. five trocars were used (2-10 mm, 2-12 mm, 1-11 mm) for video laparoscopic access to the peritoneal cavity. the operations were performed without complication in 62-95 min (mean +/- sem, 77 +/- 7 min). each animal tolerated oral feedings on the first post ...	1994	8153866
seasonal effects on fertility in gilts and sows.	the ancestral wild pig is a short day length seasonal breeder. the domestic pig appears to have retained some of this seasonality as evidenced by a reduction in fertility during the summer-autumn period. the most important aspect of this seasonality is a reduction in the number of mated sows that farrow. many of these sows conceive and embryos develop normally for 20-25 days before pregnancy is terminated and the sow returns to oestrus (25-35 days after mating). in other species, transduction of ...	1993	8145204
regional vasodilating properties of isoflurane in normal swine myocardium.	studies of the coronary vasodilating properties of isoflurane have produced inconsistent results. isoflurane has been reported to cause minimal or no coronary vasodilation, mild dose-related vasodilation, or even near-maximal coronary vasodilation. the current study was performed to clarify the direct coronary vasodilating potency of isoflurane.	1994	8141453
sodium-taurocholate-induced acute necrotizing pancreatitis does not affect jejunal oxygenation in pigs.	to study the influence of experimentally induced acute necrotizing pancreatitis on jejunal oxygen transport, jejunal oxygen consumption, and mucosal po2.	1994	8124956
collagens in the injured porcine intervertebral disc.	spinal pain often is thought to be due to degeneration and mechanical failure of the intervertebral disc. since the mechanical strength of the tissue depends on collagen fibers, the present study was designed to investigate the reactions in collagen metabolism after an experimentally induced disc injury. five domestic pigs underwent an incision in the anterior part of the annulus fibrosus of disc l4-l5 through a retroperitoneal approach. the animals were killed 3 months postoperatively, and the ...	1994	8113947
inosine monophosphate dehydrogenase from porcine (sus scrofa domestica) thymus: purification and properties.	1. imp dehydrogenase (ec 1.1.1.205) from porcine thymus glands has been purified to homogeneity. 2. the enzyme has a subunit mw of 57 kda and an amino acid composition similar to those obtained from other normal and cancerous mammalian cells. 3. the apparent km values at ph 8.0 for imp and nad+ are 7 and 16 microm, respectively. 4. gmp, xmp and amp are competitive inhibitors towards imp and ki values of 50, 85 and 282 microm, respectively. 5. the effectiveness of nucleotides to protect inactivat ...	1993	8102965
distribution of hydroxylated vitamin d metabolites [25ohd3 and 1,25(oh)2d3] in domestic pigs: evidence that 1,25(oh)2d3 is stored outside the blood circulation?	1. the distribution of 25-hydroxycholecalciferol (25ohd3) and 1,25-dihydroxycholecalciferol [1,25(oh)2d3] in various organs from domestic pigs was examined by hplc. 2. plasma levels of both metabolites corresponded to those found in healthy human subjects. 3. tissue concentrations of 25ohd3 in fat, kidney, liver, and intestinal mucosa were low (< 1/3 of plasma levels), whereas tissue concentrations of 1,25(oh)2d3 exceeded plasma levels by factors 3-7, adipose tissue concentrations being the high ...	1993	8097149
the origin and genetic differentiation of native breeds of pigs in southwest china: an approach from mitochondrial dna polymorphism.	mitochondrial dna (mtdna) of six breeds of native domestic pigs from yunnan province, southwest china, and two wild boars obtained from sichuan, china, and vietnam was analyzed using 20 restriction endonucleases that recognize six nucleotides. restriction maps were made by double-digestion methods and polymorphic sites were located on the map. according to their mtdna restriction types, all the breeds were classified into six groups. genetic distances among groups were calculated to define their ...	1993	8097084
isolated in-vitro perfusion of pig hearts obtained from the abattoir: an alternative to animal experiments?	isolated pig hearts (german farm pigs) were characterized after global in-vivo ischaemia as a potential alternative to in-vivo animal studies. hearts were harvested from adult farm swine at the abattoir 10.3 +/- 2.1 min after incision of the carotid artery. they were immediately perfused and thereafter stored in ice-cold cardioplegic (st thomas's) solution. after 38 +/- 3 min, retrograde perfusion was started with oxygenated pig blood (37 degrees c; 5000 u heparin.l-1; ph 7.38 +/- 0.1; 11 mmol g ...	1994	8088275
variation in size of ag-nors and fluorescent rdna in situ hybridization signals in six breeds of domestic pig.	variation of the size of silver-stained nucleolar organizer regions (nors) of chromosomes 10 and 8 was studied in pigs of six breeds (sus scrofa l.). the silver deposits were quantified by image analysis and the results were normalized for each ag-nor chromosome. in general, normalized values for chromosomes 10 were higher than those for chromosomes 8, suggesting that the nor activity of chromosomes 10 is dominant as compared to that of chromosomes 8. however, high values for chromosomes 8 were ...	1994	8083060
enzyme activities of glycogen metabolism and mitochondrial characteristics in muscles of rn- carrier pigs (sus scrofa domesticus).	high glycogen content and abnormal mitochondria have been seen in muscles from rn- carrier pigs in a previous work. glycogen synthase, branching enzyme, phosphorylase and debranching enzyme activities, and mitochondrial characteristics were studied in normal and rn- carrier pigs. branching enzyme activity was higher (p < 0.01) and glycogen synthase activity tended to be higher in longissimus dorsi muscle from rn- carrier pigs compared to normal pigs. there were no differences in the activities o ...	1994	8081656
[synaptonemal complex analysis of 13/17 robertsonian translocation in the domestic pig].	g and c-banding study of the chromosomes of several male pigs, indicate that all of these pigs carry rob(13;17). the karyotype is 2n = 37, xy, rob (13;17). in this paper, silverstained synaptonemal complexes (scs) in surface-spread pachytene nuclei from these pigs are analysed by electron microscope. the result is as follows: robertsonian translocation metacentrics rob (13;17) paired with homologous acrocentrics (13,17), forming a trivalent. all trivalents are in a cis configuration and there is ...	1994	8080664
the prevalence of intestinal protozoa in wild and domestic pigs.	we examined faecal samples from 842 domestic and 91 wild pigs in order to record the prevalence of intestinal protozoa. we used staining of faecal smears by noller-westphal-gönnert method, cultivation in modified les broth and microscopical examination of native preparations using small magnification (for detection of balantidium coli). moreover, the faeces from wild pigs were examined by flotation in sheather's sugar solution for detection of coccidia. we found 8 coccidian species in the wild p ...	1994	8073586
regional vasodilating properties of isoflurane in stunned swine myocardium.	studies about the coronary vasodilating properties of isoflurane indicate that this drug induces coronary vasodilation. no work has examined isoflurane-induced vasodilation in known stunned myocardium. this study was conducted to determine isoflurane's coronary vasodilation potency in stunned myocardium and to compare the results obtained with normal myocardium. we determined the vasodilating properties of isoflurane in regionally perfused swine myocardium. six domestic swine were anesthetized w ...	1994	8069032
use of the domestic pig as a model for oral bioavailability and pharmacokinetic studies.		1994	8068871
hippocampus of the domestic pig: a stereological study of subdivisional volumes and neuron numbers.	with the aim of establishing a quantitative structural basis for comparative and experimental studies, the volumes of the hippocampal subdivisions and the total number of neurons in each subdivision were estimated in domestic pig brains using modern stereological techniques. in addition to a detailed description of the stereological methods used in the analysis, comprehensive descriptions of the architectonic boundaries of the subdivisions are included. the absolute and relative volumes of the s ...	1994	8061750
enhancement effects of a hepatocyte receptor-specific mr contrast agent in an animal model.	the enhancement characteristics of the liver and spleen produced by a hepatocyte-specific magnetic resonance imaging agent, an arabinogalactan-coated ultrasmall superparamagnetic iron oxide derivative, bms 180550, were evaluated. both heavily t1- and t2-weighted sequences were used. imaging was performed in the farm pig model, as a function of contrast agent concentration (5, 10, and 20 mumol of iron per kilogram) and delay (immediate, 0.5, 2.5, 5.0, 7.5, and 9.0 hours) after bolus injection of ...	1994	8061429
skeletal muscle oedema and muscle fibre necrosis during septic shock. observations with a porcine septic shock model.	in domestic pigs, intermitted application of escherichia coli-endotoxin was used to create an animal model for a prolonged hypo- and hyperdynamic septic shock-like state and to investigate mechanisms of multiple organ failure. here, we describe the changes in skeletal muscle after 18 h (2 animals) and 48 h (6 animals) of septic shock. two pigs for each observation period that received physiologic saline solutions instead of endotoxin served as controls. the earliest lesions were endothelial cell ...	1994	8055160
proopiomelanocortin gene expression during pig pituitary and brain development.	the expression of proopiomelanocortin (pomc) mrna, which plays an important role in neural, endocrine, neuroendocrine and immune systems, was studied by in situ hybridization during the development of pituitary in the domestic pig. the pomc gene was activated as early as fetal day 30 (e30). the signal for pomc mrna in the anterior lobe progressively increased from e30 to e80 and then remained at relatively constant level. in contrast, pomc transcripts in the intermediate lobe first appeared at e ...	1994	8049719
collagenase during burn wound healing: influence of a hydrogel dressing and pulsed electrical stimulation.	epithelialization of second-degree burn wounds is known to be accelerated by topical treatment with hydrogel dressings and further enhanced by pulsed electrical stimulation compared with no treatment (air exposure). tissue collagenase has been proposed to be involved during the process of epithelialization. in the present study collagenase levels were examined in partial-thickness burn wounds in the skin of four domestic pigs. collagenase levels, assayed on postburn days 1 to 10, were substantia ...	1994	8047605
expression of type i, iii, and vi collagen mrnas in experimentally injured porcine intervertebral disc.	young domestic pigs were incised with a scalpel blade into the anterior part of annulus fibrosus of lumbar discs in order to study the reparative processes in the annulus fibrosus and the secondary reactions in the nucleus pulposus. northern and slot-blot hybridizations were used to investigate type i, iii, and vi collagen gene expression in the disc tissue. for this purpose a method for rna isolation was modified so as to be applicable to the intervertebral disc, which has a low cell density an ...	1994	8039387
the impact of gelatin-resorcinol glue on aortic tissue: a histomorphologic evaluation.	although gelatin-resorcinol-formaldehyde glue has been used to treat acute aortic dissections for some time, concerns about formaldehyde's mutagenicity and carcinogenicity made it imperative to develop a new glue compound. gelatin-dialdehyde glue was produced by omitting the formaldehyde component and replacing it with two less toxic aldehydes, glutaraldehyde and glyoxal. this study evaluated the histomorphologic effects of the new glue through in vivo use on the aortic tissue of domestic pigs.	1994	8028087
a quantitative histochemical study of the spatial distribution of intrafascicular fibre types in the porcine masseter and soleus muscles.	the distribution of intrafascicular type i and ii muscle fibres together with proportions of edge- and centrally located type i and ii fibres within whole fascicles were analysed by myosin atp-ase histochemistry in 241 porcine masseter fascicles (six masseter muscles) and compared with result from 63 pig soleus fascicles (five soleus muscles). all fascicles were from 11 domestic pigs (1 yr old, 70-90 kg body weight, all female). the proportions of type i fibres (slow) and type ii fibres (fast) o ...	1994	8024493
[myocyte transformation in autologous transplantation of colonic smooth muscle in domestic pigs].	autologous gut smooth muscle graft of two piglets was used for creating a ring in sigmoidostomy. the graft was investigated several times by histology and electron microscopy. a sort of myofibroblasts were observed the 7th day in the smooth muscle. three months later the smooth muscle was completely transformed into a cellular connective tissue comprising myofibroblasts and fibroblasts. the experiment points to possible relation of smooth muscle cell, myofibroblast and fibroblast.	1994	8020118
classical swine fever: genetic detection and analysis of differences between virus isolates.	two pairs of oligonucleotide primers were designed that specifically amplified regions of the classical swine fever virus genome. these products, corresponding to a 671 bp portion of the genes encoding the e1 and e2 (gp33 and gp55) proteins and a 1090 bp portion of the putative polymerase gene, were amplified from eight virus isolates which had been responsible for a series of classical swine fever outbreaks in italy involving both domestic pigs and wild boar. for each virus the fragments were p ...	1994	7996138
[intrachromosomal mapping of the system e locus of blood groups in wild and domestic pigs].		1994	7987233
efficacy of topical cooling in lung preservation: is a reappraisal due?	the aim of the present study was to test the efficacy of topical cooling as the only viable lung preservation method using the most challenging evaluation method, namely single-lung transplantation followed by immediate contralateral pneumonectomy. ten domestic pigs (5 donors and 5 recipients) with a mean body weight of 57 kg (range, 53 to 59 kg) were used. after we administered systemic heparin (4 mg/kg), the lungs were harvested and placed in an atelectatic state under cold (8 degrees to 9 deg ...	1994	7979731
[trichinellosis in slaughtered and wild animals in switzerland using a digestion method and a serologic method (e/s-elisa)].	for many decades trichinellosis has not been reported among swiss domestic pigs. considering the fact that trichinella occurs in a sylvatic cycle in switzerland, a study was designed to reevaluate the present epidemiologic situation by investigating 10,904 fattening pigs, 218 pigs with free access to pasturage or being kept on an alp, 104 domestic boars, 106 horses, 44 wild boars and 538 foxes using a direct and an indirect diagnostic technique (digestion method and serology with elisa and an ex ...	1994	7973563
comparison of retrograde endo-pyelotomy and endo-balloon rupture of the ureteropelvic junction in a porcine model.	excellent results and durable success have been achieved with antegrade and retrograde endo-pyelotomy for treating primary and secondary ureteropelvic junction obstruction. recently, a 30f dilating balloon was used to rupture the ureteropelvic junction (endobrst) with encouraging results. while balloon distention of the ureteropelvic junction is a technically simpler procedure than endo-pyelotomy, clinical and laboratory data comparing the 2 methods are lacking. in an acute and chronic animal st ...	1994	7966717
imaging of exocrine pancreatic function. investigation of the bioavailability of weak organic acids as potential pancreatic contrast agents for computed tomography.	the feasibility of targeting iodinated contrast agents to the exocrine pancreas was investigated. iodinated weak organic acids including succinic acid-mono-3-amino-2,4,6-triiodo- n-ethylanilide (compound i), the ethanolamine salt of n-ethylsuccinic acid-(2,4,6-triiodo-3-methylamino anilide) (compound ii), and the sodium salt of 2,4,6-triiodo-3-n-ethylacetylamino-phenylpropionic acid (compound iii) were studied as potential contrast agents for computed tomography (ct) of the pancreas.	1994	7960615
effect of ventilation on acid-base balance and oxygenation in low blood-flow states.	to investigate how minute ventilation affects the partial pressure of end-tidal co2 and arterial and mixed venous ph, pco2, po2, and the concentration of bicarbonate during low blood-flow states. we tested the null hypothesis that acid-base conditions during low rates of blood flow are not significantly different when minute ventilation is doubled or halved.	1994	7956288
effect of vest cardiopulmonary resuscitation on cerebral and coronary perfusion in an infant porcine model.	to determine cerebral and myocardial blood flow rates during vest cardiopulmonary resuscitation (cpr) without direct cardiac compression in an infant porcine model. also, to determine if circumferential chest compression without the chest deformity ordinarily associated with precordial compression maintains cerebral and myocardial blood flow rates during prolonged cpr. finally, to establish the effect of compression rate and duty cycle on cerebral and myocardial blood flow rates during vest cpr ...	1994	7956287
[experimental studies of laparoscopically-assisted rectum resection with colo-anal or colorectal anastomosis].	a minimally invasive procedure for radical resection of tumors in the deep rectum without laparoscopic anastomosis was developed in seven domestic pigs. the length of resected rectosigmoid and mesenterium was adequate on all oncologic counts.	1994	7934583
morphologic characteristics of lesion formation and time course of smooth muscle cell proliferation in a porcine proliferative restenosis model.	this study was performed to define the evolution of lesion morphology and its relation to thrombus formation and smooth muscle cell proliferation after experimental coronary stent placement.	1994	7930266
removal of the preputial diverticulum in swine.	a new technique for removal of the preputial diverticulum in swine was performed on 33 sexually intact male potbellied pigs, 12 domestic boar cadavers, and 4 domestic swine (2 sexually intact males and 2 barrows). with each pig under general anesthesia, the body and horns of the diverticulum were everted through the preputial orifice and excised. in some cases, the stump of the diverticulum was sutured. remaining tissues were allowed to retract through the preputial orifice. the technique was mo ...	1994	7928559
site-specific dexamethasone delivery for the prevention of neointimal thickening after vascular stent implantation.	site-directed pharmacologic therapy using drug-impregnated polymers may achieve high local tissue levels at sites of arterial injury without systemic side effects. the aim of this study was to determine whether sustained, local administration of the synthetic glucocorticoid dexamethasone reduces the severity of intimal hyperplasia induced by arterial stenting in a porcine model of restenosis.	1994	7921375
topical zinc oxide treatment increases endogenous gene expression of insulin-like growth factor-1 in granulation tissue from porcine wounds.	application of zinc oxide has been shown to accelerate the healing of both chronic and acute wounds, but the mechanisms are unknown. we quantified the gene expression (mrna) for one important growth factor, insulin-like growth factor-1 (igf-1) in 12 full-thickness wounds in each of three domestic pigs treated with or without topical zinc oxide. we used a rnaase protection/solution hybridisation technique to measure igf-1 mrna concentrations, which were 50% higher in the granulation tissue in wou ...	1994	7899834
a comparative study of open, laparoscopic intracorporeal, and laparoscopic assisted low anterior resection and anastomosis in pigs.	this study was done to investigate whether laparoscopic intracorporeal (li) or laparoscopic assisted (la) colon resection results in improved anastomotic healing compared with open colon resection (or). thirty-six domestic swine were randomly assigned to undergo either li, la, or or of the rectosigmoid. for or cases, the sigmoid was resected through a midline incision, and a transanal end-to-end stapled anastomosis was constructed with an ils device. for la and li cases, the sigmoid was laparosc ...	1995	7887528
heat and social stress effects on pig immune measures.	forty-eight domestic pigs were used to evaluate the effects of heat and social stress on immune indices. pigs were brought together in groups of three per pen and video-taped for the first 72 h. video tapes were viewed to determine time spent in aggressive and submissive behaviors. social status of each pig was determined from outcomes of agonistic interactions. pens of pigs were housed in either a thermoneural (control, 24 degrees c) or heat-stress (33 degrees c) air temperature. immune measure ...	1994	7883617
5-ht3 receptor antagonism by anpirtoline, a mixed 5-ht1 receptor agonist/5-ht3 receptor antagonist.	1. the aim of this study was to provide evidence that anpirtoline, which is an agonist at 5-ht1b and 5-ht1d receptors and also displays submicromolar affinity for 5-ht1a recognition sites, in addition, acts as an antagonist at 5-ht3 receptors. 2. in radioligand binding studies on rat brain cortical membranes, anpirtoline inhibited specific binding of [3h]-(s)-zacopride to 5-ht3 receptor recognition sites (pki: 7.53). 3. in n1e-115 neuroblastoma cells in which [14c]-guanidinium was used as a tool ...	1995	7881726
isolation of batai virus from sentinel domestic pig from kolar district in karnataka state, india.	batai virus was isolated from the blood sample of one sentinel piglet out of 34 used to monitor the activity of the japanese encephalitis (je) virus at madikere village in kolar district, karnataka state, india. this is the first report of the isolation of batai virus from a mammal.	1994	7879716
persistence of haemagglutination-inhibition antibodies to je and wn viruses in naturally infected domestic pigs in karnataka state, india.	domestic pigs were used as sentinels to monitor the prevalence of japanese encephalitis (je) and west nile (wn) viruses in kolar district of karnataka state, india. the study revealed that the pigs once seroconverted to je and wn viruses, remained immune as long as they were available for observation, up to three years.	1994	7879715
[effects of pollution with fluoride on bone dynamics of periosteum in iliac of domestic pigs].	studies on bone dynamic parameters of iliac periosteum and endosteum in domestic pigs with fluorosis caused by coal-burning contamination were conducted. bone dynamics of 12 pigs with experimental fluorosis and nine controls both labelled with double tetracycline was investigated. results showed the distance between two labels on iliac periosteum of domestic pigs with fluorosis shortened and, rates of mineralization and bone matrix formation slowed. it suggested fluorosis played significantly in ...	1994	7867457
long term bradycardia by electrical pacing: a new method for studying heart rate reduction.	the aim was to develop a method of inducing bradycardia on a chronic basis by electrical pacing and to study its effect upon myocardial capillarity in pigs.	1994	7867029
emesis induced in domestic pigs: a new experimental tool for detection of antiemetic drugs and for evaluation of emetogenic potential of new anticancer agents.	the domestic pig was used to develop a new model for evaluating the emetogenic potential of anticancer drugs and determining the antiemetic activity of drugs. emesis was characterized by expulsion of solid or liquid material. in each animal, the number of vomits after infusion of the emetogenic drug (infusion in ketamine and xylazine anesthesia) was recorded in 1-hr periods during the first 4 hr and then in a 4- and a 16-hr period. intravenous infusion of cisplatin caused a concentration-depende ...	1994	7865862
light microscopic distribution of some cholinergic markers in the rat and rabbit locus coeruleus and the nucleus angularis grisea periventricularis of the domestic pig (sus scrofa): a correlative electron microscopic investigation of cholinergic receptor proteins in the rabbit.	cholinergic modulation of locus coeruleus (lc) neurons evokes a variety of neuronal and behavioural effects. in an attempt to understand the lc cholinergic circuit, several markers has been investigated and compared. (immuno)-histochemical and autoradiographic methods have been used on rat, rabbit, and pig tissue. to identify the boundaries of the lc in each of these species, sections through the entire brainstem have been stained for tyrosine hydroxylase. the results indicate that the pig does ...	1994	7849322
dissociation between anti-infarct effect and anti-edema effect of ischemic preconditioning.	this study tested the hypothesis that preconditioning, by reducing catabolite accumulation during ischemia, reduces osmotic swelling and myocardial necrosis during subsequent reperfusion. farm pigs were randomly allocated to one of three groups of treatment: a control group undergoing a 48-min coronary occlusion (co) of the middle left anterior descending artery, a preconditioned group (2 cycles of 5-min co and 5-min reperfusion before the 48-min co), or an intracoronary perfusion group receivin ...	1995	7840267
coronary blood flow autoregulation and flow heterogeneity in the stunned heart.	we used an anesthetized swine model of regionally "stunned" myocardium to determine the effect of stunning on coronary autoregulation and blood flow heterogeneity. in 18 domestic swine, stunning was accomplished by reducing blood flow to the left anterior descending coronary artery (lad) by approximately 75% of baseline for 15 min and restoring it to normal for 1 hour. we quantified coronary autoregulation using both the slope of coronary pressure-flow curves and an autoregulation index. we quan ...	1994	7830329
glycogen depletion according to muscle and fibre types in response to dyadic encounters in pigs (sus scrofa domesticus)--relationships with plasma epinephrine and aggressive behaviour.	changes in glycogen content according to fibre type were assessed in a predominantly white (longissimus) and a predominantly red (semispinalis) pig muscle, in response to dyadic encounters involving aggressive interactions. tested animals showed significantly lower glycogen levels than the control in the semispinalis, but not in the longissimus muscle. histological treatment of muscle serial cuts followed by computerized image analysis showed that the observed decrease in muscle semispinalis gly ...	1994	7828029
agonistic behavior, plasma stress hormones, and metabolites in response to dyadic encounters in domestic pigs: interrelationships and effect of dominance status.	agonistic behavior, neuroendocrine, and plasma metabolite changes were studied in 16 domestic large white pigs (100 +/- 5 kg) submitted to dyadic encounters (30 min) in a neutral environment. the animals had been housed individually for 2 months prior to the experiment. aggressive and submissive behaviors were recorded for each animal during the encounter. surgically implanted catheters allowed collection of blood samples at selected times. plasma levels of cortisol, catecholamines, and metaboli ...	1994	7824582
experimental studies on the susceptibility of domestic pigs to west nile virus followed by japanese encephalitis virus infection and vice versa.	a study on the susceptibility of domestic pigs to west nile virus (wnv) and japanese encephalitis virus (jev) infection was carried out. one batch of pigs was inoculated with wnv followed by jev and another batch was inoculated vice versa. the first batch developed low level of viraemia and haemagglutination-inhibition (hi) antibodies to both viruses. there was a booster effect on the already existing wnv antibodies after challenging with jev. in the second batch the animals developed high level ...	1994	7817897
[the specificity of blastocysts (rhizopoda: lobosea)].	the analysis of specificity of blastocysts was based on the data on host association and on experimental data. in experiments we failed to infect the geese (anser anser) with blastocystis galli taken from the fowl (gallus gallus) and also failed to infect the fowl with b. suis taken from the pigs (sus scrofa domestica). experimental data and field observations of blastocysts distribution among different groups of hosts point out that the same species of blastocysts can not parasitize in hosts be ...	1994	7816501
melatonin and farm animals: endogenous rhythms and exogenous applications.	studies on farm animals have contributed significantly to our increased understanding of basic melatonin-related physiological mechanisms, as well as to the regulation of reproduction and pelage in individual domestic species. this review concentrates on recent work on the role of melatonin in the regulation of porcine reproduction, cervine endocrine, and behavioral cycles and wool and cashmere production which has added to this knowledge base. early studies of the domestic pig indicated that me ...	1994	7807370
a closed-chest pig model of sustained ventricular tachycardia.	the goal of this study was to develop and explore a closed-chest animal model of sustained vt. seven of 11 domestic pigs had successful induction of myocardial infarction by injection of agarose gel microbeads into the left anterior descending coronary artery through an inflated balloon angioplasty catheter. four of the first five pigs died and seem to represent a "learning experience." during a 3- to 50-day follow-up period, each pig underwent 1-3 electrophysiological studies. sustained, monomo ...	1994	7800561
laparoscopic partial splenic resection.	twenty domestic pigs with an average weight of 30 kg were subjected to laparoscopic partial splenic resection with the aim of determining the feasibility, reliability, and safety of this procedure. unlike the human spleen, the pig spleen is perpendicular to the body's long axis, and it is long and slender. the parenchyma was severed through the middle third, where the organ is thickest. an 18-mm trocar with a 60-mm endopath linear cutter was used for the resection. the tissue was removed with a ...	1995	7773460
pig trypanosomosis: prevalence and significance in the endemic middle belt zone of southern nigeria.	abattoir and field/market surveys of 1,954 crossbred pigs aged 6 to 30 months, for trypanosomosis in the middle belt zone of southern nigeria, revealed a 26.8 infection rate. of those infected, 66.5, 23.9 and 8.2 % were due to mixed, single trypanosoma brucei and t. congolense infections respectively. although 1.5 % of the infections were unidentified, there was no evidence of t simiae. the infection rate was significantly higher (p < 0.05) among the abattoir pigs (37.8 %) than among the farm pi ...	1994	7770662
effect of testosterone treatment on vasoconstrictor response of left anterior descending coronary artery in male and female pigs.	androgens may be risk factors in the pathogenesis of coronary artery disease by promoting coronary vasoconstriction. we studied the effect of testosterone treatment on coronary vascular reactivity of male and female domestic pigs treated for 2 weeks with either placebo or testosterone 10 mg/kg subcutaneous (s.c.) pellets. vascular reactivity was studied in ring segments (4-5 mm) isolated from the left anterior descending coronary artery (lad). no significant sex difference was noted in the respo ...	1995	7769819
myocardial technetium-99m-teboroxime activity in acute coronary artery occlusion and reperfusion: relation to myocardial blood flow and viability.	the purpose of this study was to test the hypothesis that 99mtc-teboroxime retention within the heart depends at least in part on the presence of viable myocytes.	1995	7769429
the pattern of coronary arteriolar bifurcations and the uniform shear hypothesis.	by minimizing the cost function, which is the sum of the friction power loss and the metabolic energy proportional to blood volume, murray derived an optimal condition for a vascular bifurcation. murray's law states that the cube of the radius of a parent vessel equals the sum of the cubes of the radii of the daughters. we tested murray's law against our data of pig's maximally vasodilated coronary arteriolar blood vessels at bifurcation points in control and hypertensive ventricles. data were o ...	1995	7762878
combination of vitamins c and e alters the response to coronary balloon injury in the pig.	restenosis is the major limitation of the long-term success of percutaneous transluminal coronary angioplasty. the process of restenosis involves repair of vascular injury and remodeling of vessel architecture. therapeutic interventions that improve vascular function may therefore be beneficial in the treatment of restenosis. antioxidants such as probucol and vitamins c and e have proved effective in improving endothelial function in hypercholesterolemia, inhibiting lipid accumulation in animal ...	1995	7749812
the pigmap consortium cytogenetic map of the domestic pig (sus scrofa domestica).		1995	7749224
olfactory sensitivity to the pheromone, androstenone, is sexually dimorphic in the pig.	sexually dimorphic pheromone pathways have been used successfully to study insect olfactory coding. as one of the few mammalian species with an identified sex pheromone, the domestic pig (sus scrofa) may be an ideal vertebrate species in which to examine sex differences in olfactory processing of a specific stimulus. in this experiment, androstenone and control odor detection thresholds were measured in adult male, female, and castrated male pigs. in an operant task, pigs were tested with descen ...	1995	7716200
sex differences in the distribution of estrogen receptors in the septal area and hypothalamus of the domestic pig (sus scrofa).	recently in the pig hypothalamus a vasopressin- and oxytocin-containing nucleus was identified which, like the supraoptic nucleus, becomes sexually dimorphic after puberty. following the increase in circulating steroids at puberty, the vasopressin- and oxytocin-containing nucleus becomes twice as large in both males and females. in adulthood, the vasopressin- and oxytocin-containing nucleus of females is approximately twice as large as that in males. because these alterations are possibly due to ...	1995	7708211
cholecystokinin-, enkephalin-, and substance p-like immunoreactivity in the dentate area, hippocampus, and subiculum of the domestic pig.	the distribution of cholecystokinin-like, enkephalin-like, and substance p-like immunoreactivities is described in the dentate area, hippocampus, and subiculum of the domestic pig (sus scrofa domesticus) as a baseline for future experimental studies. the distributions in the pig are compared with previous observations in other species. cholecystokinin-like immunoreactive nerve cell bodies were intensely stained and present in large numbers in all subfields studied. cholecystokinin-like immunorea ...	1993	7685777
preretinal and optic nerve head neovascularization induced by photodynamic venous thrombosis in domestic pigs.	to develop a reliable model of ischemic preretinal and optic nerve head neovascularization in young male domestic pigs.	1993	7682408
mechanisms of distamycin a/dapi chromosome staining. i. competition binding effects of nonintercalative dna groove-binding agents in situ and in vitro.	the molecular mechanism underlying distamycin a-induced differential dapi fluorescent staining of metaphase chromosomes was studied in sus scrofa domestica both cytologically, using, besides dapi, two isomeric derivatives of dapi (d288.45 and d288.48), and molecularly, by in vitro competitive-binding studies using s. scrofa satellite dna and synthetic dna polymers. significant differences in heterochromatin staining were observed between d288.45 and d288.48. distinct distamycin a/dapi bands were ...	1993	7678548
dynamics of myocardial metabolism in the preconditioned porcine heart studied using continuous microdialysis.	a microdialysis technique was used to measure myocardial catabolism of adenine nucleotides during preconditioning. following thoracotomy in domestic pigs, a probe was inserted into the ischaemic myocardium and two probes into normal myocardium. preconditioning was instituted by occluding a diagonal branch of the left anterior descending coronary artery four times over a 10 min period (interspersed with 20 min of reperfusion each time), followed by index ischaemia for 40 min and terminated by 20 ...	1995	7671904
dopamine-1-receptor stimulation and mucosal tissue oxygenation in the porcine jejunum.	to evaluate the effects of dopamine-1-receptor stimulation on intestinal mucosal tissue oxygenation.	1995	7664559
dysregulation of the veno-arterial response in the superior mesenteric artery during endotoxic shock.	to investigate whether the vascular dysfunction in endotoxic shock is associated with inhibition of the veno-arterial response of the superior mesenteric artery.	1995	7664554
the need for ventilatory support during bystander cpr.	to compare cpr with chest compressions plus ventilatory support (cc+v) and chest compressions alone (cc).	1995	7661426
mechanical ventilation may not be essential for initial cardiopulmonary resuscitation.	in a rodent model of cardiac arrest and resuscitation in which the inspired gas mixture was enriched with oxygen, resuscitability and survival were unaffected by positive pressure ventilation. in the present study, in a larger animal model, tidal volumes generated during precordial compression and with spontaneous gasping were quantitated.	1995	7656640
functional characteristics of a new coronary perfusion balloon: comparison with the flowtrack 40 in closed chest swine.	this study was designed to compare a new investigational coronary perfusion balloon (wave, scimed life systems inc.) with that of an approved, widely used, current generation device (flowtrack 40, advanced cardiovascular systems). domestic swine were anesthetized with pentobarbital and instrumented using standard percutaneous technique. left ventricular contractile function (dp/dt) was monitored using a micromanometer while intracoronary and surface ecgs were also recorded. eight target vessels ...	1995	7656316
allopurinol improves myocardial reperfusion injury in a xanthine oxidase-free model.	the ability of allopurinol to protect against reperfusion injury in the heart has usually been attributed to its xanthine oxidase (xo)-inhibiting properties. human myocardium however, has exhibited low levels of xo activity. to investigate the effects of allopurinol in an xo-free model and determine whether pretreatment is necessary, 12 domestic pigs (15 kg to 20 kg) underwent occlusion of the left circumflex for 8 minutes followed by reperfusion for 4 hours. one group received allopurinol infus ...	1995	7636893
does hypoxia or hypercarbia independently affect resuscitation from cardiac arrest?	in a previous cardiopulmonary resuscitation (cpr) study in swine, ventilation was associated with improved rate of return of spontaneous circulation (rosc) compared with nonventilated animals, which had greater hypoxia and hypercarbic acidosis. we used the same model to determine the independent effect of hypoxia and hypercarbic acidosis on rosc after cardiac arrest.	1995	7634893
chronic effects of misoprostol in combination with the nsaid, diclofenac, on gastrointestinal tract of pigs. relation to diarrheagenic activity, leukocyte infiltration, and mucosal leukotrienes.	to determine the mode of protective effects of misoprostol against the chronic gastrointestinal ulceration from the nsaid, diclofenac, studies were undertaken in domestic pigs, a model of human gastrointestinal ulceration, to determine (1) the effects of repeated daily dosing for 10 days of diclofenac 5 mg/kg/day twice a day (as voltaren tablets) on the gastrointestinal morphology, 59fe-red blood loss, mucosal myeloperoxidase (mpo) activity (as an indicator of leukocyte infiltration), and mucosa ...	1995	7628265
swine model of haemophilus ducreyi infection.	haemophilus ducreyi is a strict human pathogen that causes sexually transmitted genital ulcer disease. we infected domestic swine with h. ducreyi 35000, resulting in the development of cutaneous ulcers histologically resembling human chancroid lesions. intraepidermal lesions progressed from pustules to ulcers containing polymorphonuclear leukocytes and were accompanied by a dermal inflammatory infiltrate containing t cells and macrophages. h. ducreyi was recovered from lesions up to 17 days afte ...	1995	7622236
percutaneous serial catheterization in swine: a practical approach.	in a model designed for experimental cryoablation of the his bundle in swine, a study that required multiple and follow-up cardiac catheterizations, femoral percutaneous cannulations were successfully performed in 14 of 15 swine: 6/6 yucatan miniature, 5/6 hanford miniature, and 3/3 yorkshire farm pigs, weight range 15-50 kg. a follow-up cardiac catheterization was performed in 10 of these animals: 5 hanford and 5 yucatan. femoral percutaneous cannulation was successful in 6. a cutdown approach ...	1995	7619782
lower esophageal sphincter pressure during prolonged cardiac arrest and resuscitation.	unprotected airway ventilation models have been based on a lower esophageal sphincter (les) pressure found in human beings under general anesthesia. whether this assumption is applicable during cardiac arrest in human beings is unknown. we attempted to determine the effects of prolonged ventricular fibrillation (vf) on the tension of the les in a swine model of cardiac arrest.	1995	7618786