Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of the bact/alert automated blood culture system for detecting bacteria and measuring their growth kinetics in leucodepleted and non-leucodepleted platelet concentrates. | to evaluate the bact/alert automated blood culture system for the detection of bacteria in platelet concentrates, and to determine bacterial growth kinetics in leucodepleted and non-leucodepleted units. | 2001 | 11703857 |
| expression of clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium. | the mrna for rvp.1 (rat ventral prostate) increases in abundance before gland involution after androgen deprivation. rvp.1 is homologous to cpe-r, the high-affinity intestinal epithelial receptor for clostridium perfringens enterotoxin (cpe), and is sufficient to mediate cpe binding and trigger subsequent toxin-mediated cytolysis. rvp.1 (claudin-3) and cpe-r (claudin-4) are members of a larger family of transmembrane tissue-specific claudin proteins that are essential components of intercellular ... | 2001 | 11691807 |
| effects of probiotic administration upon the composition and enzymatic activity of human fecal microbiota in patients with irritable bowel syndrome or functional diarrhea. | in a clinical trial, 10 patients suffering from irritable bowel syndrome or functional diarrhea were administered the probiotic preparation vsl-3. preliminary results indicated that administration of vsl-3 improved the clinical picture and changed the composition and biochemistry of fecal microbiota. titer variations of intestinal bacterial groups were evaluated by culture and pcr techniques. a significant increase in lactobacilli, bifidobacteria and streptococcus thermophilus was observed as a ... | 2001 | 11686387 |
| the effects of mesenteric ischemia on ileal colonization, intestinal integrity, and bacterial translocation in newborn piglets. | the effects of mesenteric ischemia on ileal colonization, intestinal integrity, and bacterial translocation (bt) in newborn piglets were investigated in 362-day-old pietrain piglets. group i, controls were not operated upon; group ii underwent a sham laparotomy; and group iii underwent ligation of the mesenteric vessels in the distal ileum. after 3 days, the kidneys, spleens, livers, and ileal segments were harvested for microbial and histologic analyses. two piglets in the ischemic group died; ... | 2001 | 11666048 |
| heat treatment adaptations in clostridium perfringens vegetative cells. | vegetative cells of clostridium perfringens enterotoxigenic strains nctc 8679, nctc 8238. and h6 were grown at 37 degrees c followed by a 60-min exposure to 28 degrees c or 46 degrees c. d10-values, as a measure of thermal resistance at 60 degrees c, were significantly lower for 28 degrees c exposures as compared with cultures given 37 and 46 degrees c exposures. following refrigeration at 4 degrees c for 24 h, d10-values for the 37 and 46 degrees c samples could not be differentiated from 28 de ... | 2001 | 11601701 |
| selective growth inhibitor toward human intestinal bacteria derived from pulsatilla cernua root. | among 21 medicinal plants, the growth-inhibiting activity of pulsatilla cernua root-derived materials toward human intestinal bacteria was examined by using an impregnated paper disk method. the biologically active components of p. cernua roots were characterized as 4-hydroxy-3-methoxycinnamic acid and 3,4-dihydroxycinnamic acid by spectroscopic analysis. the activity was compared with that of six commercially available cinnamic acid derivatives trans-cinnamaldehyde, trans-cinnamic acid, cinnamy ... | 2001 | 11600003 |
| efficacy of in-feed tylosin phosphate for the treatment of necrotic enteritis in broiler chickens. | the efficacy of tylosin phosphate for the treatment of necrotic enteritis (ne) was investigated in a floor pen study of 2,000 broiler chickens. a model in which clostridium perfringens was administered in the feed on days 14 to 16 was used to initiate an outbreak of ne. treatments, allocated at the pen level in a randomized complete block design, consisted of five levels of tylosin phosphate (0, 50, 100, 200, or 300 ppm) administered in the feed on days 15 to 22, following the identification of ... | 2001 | 11599704 |
| naturally occurring clostridium perfringens nontoxic alpha-toxin variant as a potential vaccine candidate against alpha-toxin-associated diseases. | clostridium perfringens mutant strain 121a/91 shows neither enzymatic (phospholipase c) nor hemolytic activity. nevertheless, the cpa gene and the corresponding alpha-toxin variant are detectable. vaccination with this genetically constructed alpha-toxin variant, rat121/91, induces antibodies capable of significantly reducing activities induced by wild-type toxin. thus, rat121/91 could be a useful vaccine candidate. | 2001 | 11598102 |
| clostridium perfringens type a enterotoxin forms mepacrine-sensitive pores in pure phospholipid bilayers in the absence of putative receptor proteins. | clostridium perfringens enterotoxin (cpe) is an important cause of food poisoning with no significant homology to other enterotoxins and its mechanism of action remains uncertain. although cpe has recently been shown to complex with tight junction proteins, we have previously demonstrated that cpe increases ionic permeability in single caco-2 cells using the whole-cell patch-clamp technique, thereby excluding any paracellular permeability. in this paper we demonstrate that cpe forms pores in syn ... | 2001 | 11597350 |
| gastrointestinal disease in the domestic setting: what can we deduce from surveillance data? | 2001 | 11597156 | |
| the complex interactions between clostridium perfringens enterotoxin and epithelial tight junctions. | clostridium perfringens enterotoxin (cpe) is responsible for the diarrheal symptoms of c. perfringens type a food poisoning and antibiotic-associated diarrhea. the cpe protein consists of a single 35 kda polypeptide with a c-terminal receptor-binding region and an n-terminal toxicity domain. under appropriate conditions, cpe can interact with structural components of the epithelial tight junctions, including certain claudins and occludin. those interactions can affect tight junction structure an ... | 2001 | 11595640 |
| mode of action of beta-barrel pore-forming toxins of the staphylococcal alpha-hemolysin family. | staphylococcal alpha-hemolysin is the prototype of a family of bacterial exotoxins with membrane-damaging function, which share sequence and structure homology. these toxins are secreted in a soluble form which finally converts into a transmembrane pore by assembling an oligomeric beta-barrel, with hydrophobic residues facing the lipids and hydrophilic residues facing the lumen of the channel. besides alpha-hemolysin the family includes other single chain toxins forming homo-oligomers, e.g. beta ... | 2001 | 11595629 |
| molecular characterization of a phosphatidylcholine-hydrolyzing phospholipase c. | while searching for a phospholipase c (plc) specific for phosphatidylcholine in mammalian tissues, we came across such an activity originating from a contamination of pseudomonas fluorescens. this psychrophilic bacterium was found to contaminate placental extracts upon processing in the cold. the secreted phosphatidylcholine-hydrolyzing plc was purified by a combination of chromatographic procedures. as substrates, the enzyme preferred dipalmitoyl-phosphatidylcholine and 1-palmitoyl-2-arachidono ... | 2001 | 11589699 |
| the major gangliosides of human peripheral blood monocytes/macrophages: absence of ganglio series structures. | sialoglycosphingolipids (gangliosides) are membrane components of eukaryotic cells that modulate cell signal transduction events. discrepancies exist in the published descriptions of the gangliosides present in the human peripheral monocyte/macrophage. macrophages were isolated from healthy human volunteers by two different methods. their ganglioside fractions were isolated and examined by 2d thin-layer mobility, enzymatic susceptibility, and mass spectral-collision induced dissociation-mass spe ... | 2001 | 11588159 |
| clostridium infection (jisizheng) in yaks in qinghai, china. | since the mid-1980s, outbreaks of a disease characterized by a sudden onset, acute deaths and extensive haemorrhages in the viscera and digestive tract of yaks have been prevalent in qilian, qinghai, china. the disease is known as jisiheng by local people. virulent clostridium perfringens type a and clostridium haemolytica were isolated from yaks that had died of jisizheng. in 1996 and 1997, yaks were immunized with a polyvalent inactivated vaccine against c. perfringens and with an inactivated ... | 2001 | 11583378 |
| functional assembly of two membrane-binding domains in listeriolysin o, the cytolysin of listeria monocytogenes. | listeriolysin o (llo) is a major virulence factor secreted by the pathogenic listeria monocytogenes and acts as pore-forming cytolysin. based on sequence similarities between llo and perfringolysin (pfo), the cytolysin from clostridium perfringens of known crystallographic structure, two truncated llo proteins were produced: llo-d123, comprising the first three predicted domains, and llo-d4, the last c-terminal domain. the two proteins were efficiently secreted into the culture supernatant of l. ... | 2001 | 11577147 |
| evidence for antibiotic induced clostridium perfringens diarrhoea. | clostridium difficile is a well documented cause of antibiotic associated diarrhoea in hospitalised patients, but may account for only approximately 20% of all cases. this leader reviews the current knowledge and understanding of the pathogenesis, epidemiology, and diagnosis of non-food borne clostridium perfringens diarrhoea. although enterotoxigenic c perfringens has been implicated in some c difficile negative cases of antibiotic associated diarrhoea, c perfringens enterotoxin detection metho ... | 2001 | 11577119 |
| toxicosis in dogs exposed to clostridium perfringens type a. | 2001 | 11570797 | |
| enteritis as a cause of mortality in the western bluebird (sialia mexicana). | increased mortalities in adult western bluebirds utilizing nestboxes were noted in western oregon during 1998 and 1999. a necrohemorrhagic enteritis was found in 8 of 10 birds submitted for necropsy. acanthocephalan parasites were present in four of eight birds with enteritis. microscopic changes consistent with necrotic or ulcerative enteritis were commonly present. anaerobic culture of the intestine yielded clostridium perfringens in three of three birds. genotype analysis of two of these isol ... | 2001 | 11569757 |
| a field study of naturally occurring specific antibodies against clostridium perfringens alpha toxin in norwegian broiler flocks. | necrotic enteritis (ne), a disease associated with high numbers of the intestinal bacterium clostridium perfringens, is common in intensive broiler production. antimicrobial feed additives may control the disease, but their use is now being questioned in many countries. a field study was undertaken at the end of 1997 to study the level of naturally occurring specific humoral immunity against phospholipase c (plc; c perfringens alpha toxin) in norwegian broiler flocks. blood samples were collecte ... | 2001 | 11569751 |
| monitoring of microbial hazards at farms, slaughterhouses, and processing lines of swine in korea. | this study was executed to investigate microbiological hazards at swine farms, slaughterhouses, dressing operations, and local markets for the application of the hazard analysis critical control point system in korea by analyzing total aerobic plate count (apc) and presence of pathogens. six integrated pig farms and meat packers were selected from six different provinces, and samples were collected from pig carcasses by swabbing and excision methods at the slaughterhouses, processing rooms, and ... | 2001 | 11563516 |
| unexplained deaths among injection drug users: a case of probable clostridium myonecrosis. | a series of unexplained deaths associated with soft-tissue inflammation and severe systemic sepsis was reported among injection drug users (idus) in the united kingdom and the republic of ireland in 2000. health canada has identified one reported fatality in an idu that matched the case definition. although the cause of the epidemic in the uk and ireland is not fully understood, contributing factors include injecting into muscle or beneath the skin, rather than directly into a vein, and the use ... | 2001 | 11563214 |
| prevalence of beta2 toxin gene of clostridium perfringens type a from diarrhoeic dogs. | 2001 | 11558663 | |
| molecular analysis of tetracycline resistance in pasteurella aerogenes. | tetracycline-resistant pasteurella aerogenes isolates obtained from the intestinal tract of swine were investigated for their tet genes by pcr analysis and hybridization experiments. in contrast to pasteurella isolates from the respiratory tract, tet(h) genes were detected in the chromosomal dna of only 2 of the 24 isolates, one of which also carried two copies of a tet(b) gene. all other p. aerogenes isolates carried tet(b) genes, which are the predominant tet genes among enterobacteriaceae. a ... | 2001 | 11557485 |
| characterization of the enzymatic component of the adp-ribosyltransferase toxin cdta from clostridium difficile. | certain strains of clostridium difficile produce the adp-ribosyltransferase cdt, which is a binary actin adp-ribosylating toxin. the toxin consists of the binding component cdtb, which mediates receptor binding and cellular uptake, and the enzyme component cdta. here we studied the enzyme component (cdta) of the toxin using the binding component of clostridium perfringens iota toxin (ib), which is interchangeable with cdtb as a transport component. ib was used because cdtb was not expressed as a ... | 2001 | 11553537 |
| glycosylation of haemagglutinin and stalk-length of neuraminidase combine to regulate the growth of avian influenza viruses in tissue culture. | the influence on virus replication in culture of the presence and location of glycosylation sites on the haemagglutinin (ha) glycoprotein of avian influenza viruses and differences in length of the stalk region of their neuraminidase (na) glycoprotein was examined using reassortant viruses. plaque size was measured in the presence or absence of bacterial neuraminidase (cpna) and/or an influenza virus na inhibitor, zanamivir, to assess the relative contribution of the na to replication efficiency ... | 2001 | 11551658 |
| effect of clostridium perfringens epsilon toxin on mdck cells. | epsilon toxin is one of the major lethal toxins produced by clostridium perfringens type d and b. it is responsible for a rapidly fatal disease in sheep and other farm animals. many facts have been published about the physical properties and the biological activities of the toxin, but the molecular mechanism of the action inside the cells remains unclear. we have found that the c. perfringens epsilon toxin caused a significant decrease of the cell numbers and a significant enlargement of the mea ... | 2001 | 11549414 |
| modulation of genotoxic enzyme activities by non-digestible oligosaccharide metabolism in in-vitro human gut bacterial ecosystems. | supplementation of the human diet with prebiotic substances such as inulin and non-digestible oligosaccharides (ndo), e.g., galacto-oligosaccharides (gos), has been associated with various health benefits. however, little information is available regarding the spatial location of their metabolism in human gut bacterial ecosystems. therefore, the present study investigated the metabolism of inulin and gos with respect to bacterial growth, bifidobacterial stimulatory properties and anti-mutagenici ... | 2001 | 11549186 |
| evaluation of microbiological quality of medicinal plants used in natural infusions. | consumption of preparations of medicinal plants has been increasing during the last decades in occidental societies. however, there are no effective sanitary controls of these products. to evaluate the nature and content of microbiological contamination, 62 samples of seven medicinal plants (chamomile, leaves of orange tree, flowers of linden, corn silk, marine alga, pennyroyal mint and garden sage) were studied, using conventional microbiological methods. practically all samples (96.8%) were co ... | 2001 | 11545215 |
| prevalence of staphylococcus aureus and staphylococcal enterotoxins in raw pork and uncooked smoked ham--a comparison of classical culturing detection and rflp-pcr. | in many countries staphylococcus aureus is considered to be the second or third most common pathogen causing outbreaks of food poisoning, only outnumbered by salmonella spp. and in competition with clostridium perfringens. often the consumption of ham or meat containing staphylococcal enterotoxins (se) is identified as cause of the illness. thus, to gain an insight into the prevalence of s. aureus and its emetic enterotoxins in raw pork and uncooked smoked ham and to investigate how the prevalen ... | 2001 | 11545209 |
| nutritional and bacteriological properties of some game duck carcasses. | the aim of this work was to evaluate the body composition, bacteriological quality, proximate composition, amino acids content, total lipids fractionation, as well as fatty acids profile in breast and thigh meat (with skin) of males and females of two species of game ducks namely: pintail (anas acuta) and garganey (anas querquedula). the obtained results are as follows. the live weight of pentail and garganey females constituted 59.0 and 86.0% of male's weight in pintail and garganey, respective ... | 2001 | 11534470 |
| group g beta-hemolytic streptococcal bacteremia characterized by 16s ribosomal rna gene sequencing. | little is known about the relative importance of the four species of lancefield group g beta-hemolytic streptococci in causing bacteremia and the factors that determine the outcome for patients with group g beta-hemolytic streptococcal bacteremia. from 1997 to 2000, 75 group g beta-hemolytic streptococcal strains were isolated from the blood cultures of 66 patients. sequencing of the 16s rrna genes of the group g beta-hemolytic streptococci showed that all 75 isolates were streptococcus dysgalac ... | 2001 | 11526143 |
| rapid confirmation of clostridium perfringens by using chromogenic and fluorogenic substrates. | the use of 4-methylumbelliferyl phosphate (mup) and ortho-nitrophenyl-beta-d-galactopyranoside (onpg) for the identification of clostridium perfringens was investigated. a liquid assay containing both mup and onpg was a highly specific alternative method for c. perfringens confirmation, reducing incubation time from 48 to only 4 h. the assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life. | 2001 | 11526053 |
| tetracycline-resistance genes of clostridium perfringens, clostridium septicum and clostridium sordellii isolated from cattle affected with malignant edema. | the minimal inhibitory concentrations (mics) of 10 antimicrobial agents against a total of 33 isolates of clostridium perfringens, clostridium septicum and clostridium sordellii from cattle affected with malignant edema in japan was determined. the low mic activities of benzylpenicillin confirm the place of benzylpenicillin as the antibiotics of choice for treatment of malignant edema. five (22%) of 23 c. septicum strains, five (71%) of seven c. perfringens strains and all strains of c. sordelli ... | 2001 | 11524166 |
| effect of global system for mobile communication (gsm)-like radiofrequency fields on vascular permeability in mouse brain. | the effect of global system for mobile communication (gsm) radiofrequency fields on vascular permeability in the brain was studied using a purpose-designed exposure system at 898.4 mhz. mice (n= 30) were given a single far field, whole body exposure for 60 minutes at a specific absorption rate of 4 w/kg. control mice were also sham-exposed (n = 10) or permitted free movement in a cage (n = 10) to exclude any stress-related effects. vascular permeability changes were detected using albumin immuno ... | 2001 | 11523936 |
| expression of bacterial beta-glucuronidase in human bile: an in vitro study. | bacterial beta-glucuronidase causes deconjugation of bilirubin diglucuronide resulting in the precipitation of calcium bilirubinate, which contributes to biliary sludge and stone formation. this process is attributed to enzyme activity produced by the aerobic enterobacteriaceae such as escherichia coli and klebsiella sp. the presence of clostridium sp. was detected in 48 of 56 intrahepatic stones by using polymerase chain reaction techniques and cultured clostridium perfringens from 14 of 18 unb ... | 2001 | 11522976 |
| claudin-4: a new target for pancreatic cancer treatment using clostridium perfringens enterotoxin. | recently, several members of the claudin family have been identified as integral constituents of tight junctions. using expression profiling, we previously found claudin-4 to be overexpressed in pancreatic cancer. because claudin-4 has been described as a receptor for the cytotoxic clostridium perfringens enterotoxin (cpe), we investigated the effect of cpe on pancreatic cancer cells. | 2001 | 11522752 |
| in vitro study of prebiotic properties of levan-type exopolysaccharides from lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis. | batch cultures inoculated with human faeces were used to study the prebiotic properties of levan-type exopolysaccharides (eps) from lactobacillus sanfranciscensis as well as levan, inulin, and fructooligosaccharide (fos). denaturing gradient gel electrophoresis of 16s rdna fragments generated by pcr with universal primers was used to analyse the cultures. characteristic changes were revealed in the composition of the gut bacteria during fermentation of the carbohydrates. an enrichment of bifidob ... | 2001 | 11518326 |
| sequence heterogeneity of the ten rrna operons in clostridium perfringens. | we have cloned and sequenced rrna operons of clostridium perfringens strain 13 and analyzed the sequence structure in view of the phylogenesis. the organism had ten copies of rrna operons all of that comprised of 16s, 23s and 5s rdnas except for one operon. the operons clustered around the origin of replication, ranging within one-third of the whole genome sequence as it is arranged in a circle. seven operons were transcribed in clockwise direction, and the remaining three were transcribed in co ... | 2001 | 11518317 |
| occurrence of anaerobic bacterial, clostridial, and clostridium perfringens spores in raw goose livers from a poultry processing plant in hungary. | anaerobic bacterial, clostridial, and clostridium perfringens spores were enumerated in raw goose liver samples taken after evisceration of the birds (eb) in the slaughterhouse and after removal of blood vessels from the liver (rbvl) in the cannery. the samples taken after rbvl had significantly higher (p < 0.05) spore counts than did those taken after eb, indicating contamination of livers during processing. the number of c. perfringens spores was one log cycle higher in the samples taken after ... | 2001 | 11510671 |
| microbiological quality of the bivalve pinctada imbricata commercialized in cumaná, venezuela. | the habitual consumption of oysters in the city of cumaná, venezuela, is made in poor sanitary conditions, therefore a study was carried out to determine the sanitary quality of the product. for this, 120 samples of oyster pinctada imbricata were collected directly from: a) fishing boats (unhandled samples) and b) after being shucked by the salesman (handled samples). unhandled samples showed the following mpn/g: faecal coliforms < 3 - 2.2 x 10, aeromonas sp. < 3 - 1.1 x 10(3), staphylococcus au ... | 2001 | 11510429 |
| development of duplex pcr assay for rapid detection of enterotoxigenic isolates of clostridium perfringens. | a duplex pcr assay was developed for the rapid and specific amplification of the alpha-toxin (phospholipase c, plc) gene and the enterotoxin (cpe) gene from clostridium perfringens. two pairs of primers were newly designed for the species identification and also for the differentiation between enterotoxin-positive and enterotoxin-negative c. perfringens strains in a single reaction. the detection by agarose gel electrophoresis yielded 2 bands of 280-bp of plc and 420-bp of cpe for all four enter ... | 2001 | 11485070 |
| alpha 2,3-sialylation of terminal galnac beta 1-3gal determinants by st3gal ii reveals the multifunctionality of the enzyme. the resulting neu5ac alpha 2-3galnac linkage is resistant to sialidases from newcastle disease virus and streptococcus pneumoniae. | enzymatic alpha 2,3-sialylation of galnac has not been described previously, although some glycoconjugates containing alpha 2,3-sialylated galnac residues have been reported. in the present experiments, recombinant soluble alpha 2,3-sialyltransferase st3gal ii efficiently sialylated the x(2) pentasaccharide galnac beta 1-3gal beta 1-4glcnac beta 1-3gal beta 1-4glc, globo-n-tetraose galnac beta 1-3gal alpha 1-4gal beta 1-4glc, and the disaccharide galnac beta 1-3gal in vitro. the purified product ... | 2001 | 11479313 |
| bile leak from duct of luschka after liver transplantation. | we report a case of bile leak from an accessory duct of luschka during cholecystectomy during liver transplantation. | 2001 | 11477364 |
| probiotic impact on microbial flora, inflammation and tumour development in il-10 knockout mice. | the enteric bacterial flora has been implicated in the pathogenesis of enterocolitis and colon cancer in c57bl/6 il-10 knockout mice. probiotic lactobacilli modify the enteric flora and are thought to have a beneficial effect on enterocolitis. we conducted a controlled feeding trial in il-10 knockout mice using the probiotic lactobacillus salivarius ssp. salivarius ucc118. | 2001 | 11472326 |
| bacterial cholangitis. | the treatment of acute bacterial cholangitis requires broad-spectrum antibiotics to cover against gram-negative aerobic enteric organisms (escherichia coli, klebsiella species, and enterobacter species), gram-positive enterococcus and anaerobic bacteria (bacteroides fragilis and clostridium perfringens). approximately 20% of patients with acute cholangitis fail to respond to conservative treatment with antibiotic therapy and require urgent biliary decompression, which is the mainstay of therapy. ... | 2001 | 11469972 |
| a prospective study of the roles of clostridium difficile and enterotoxigenic clostridium perfringens in equine diarrhoea. | faecal samples from adult horses and from foals with diarrhoea or with normal faeces were evaluated for the presence of clostridium difficile, c. difficile toxins, c. perfringens enterotoxin (cpe) and c. perfringens spore counts. clostridium difficile was isolated from 7/55 horses (12.7%) and 11/31 foals (35.5%) with colitis, but from 1/255 normal adults (0.4%) and 0/47 normal foals (p<0.001). clostridium difficile toxins a and/or b were detected in 12/55 diarrhoeic adults (21.8%) and 5/30 diarr ... | 2001 | 11469775 |
| the roles of clostridium difficile and enterotoxigenic clostridium perfringens in diarrhea in dogs. | in this prospective study, feces of dogs with diarrhea were compared with feces of normal dogs for the presence of clostridium difficile, c difficile toxins a and b, c perfringens, and c perfingens enterotoxin (cpe). c difficile toxins a, b, or both were present in feces of 18 of 87 (21%) dogs with diarrhea and 4 of 55 (7%) normal dogs (p = 0.03), whereas cpe was present in the feces of 24 of 87 (28%) dogs with diarrhea and 3 of 55 (5%) normal dogs (p = 0.01). c difficile was isolated from 2 of ... | 2001 | 11467596 |
| evaluation of fluorogenic tsc agar for recovering clostridium perfringens in groundwater samples. | clostridium perfringens is widely recognised as a reliable water pollution indicator. since several media can be employed for the membrane filtration enumeration of this microorganism, the main aim of this work was to investigate the ability of fluorocult-supplemented tsc-agar (merck) for recovering cl. perfringens from public springs used for direct human consumption. cl. perfringens recovery was also performed on mcp agar (cultimed) according to directive 98/83 as well as on tsc-agar (merck), ... | 2001 | 11464756 |
| sphenoid sinusitis caused by clostridium perfringens. | anaerobic bacterial infections in chronic sinusitis are well described in literature. we present what is believed to be the first reported case of clostridium perfringens presenting as the causative pathogen in paranasal sinusitis. this patient presented with severe headaches and, with ct and mri findings of unilateral sphenoid sinus opacification, with bone demineralization and intrasinus calcification. this patient responded to endoscopic debridement and long-term antibiotics without sequelae. | 2001 | 11458224 |
| clostridium bacteremia: emphasis on the poor prognosis in cirrhotic patients. | bacteremic episodes caused by anaerobes are unusual and the clinical importance of clostridium bacteremia remains unclear. this retrospective case study examined the risk factors among a group of patients who developed clostridium bacteremia. medical records from 73 episodes of clostridial bacteremia in 73 patients treated in a medical center during an 11-year period were reviewed. of all episodes, 96% were community-acquired. twelve percent of patients had polymicrobial bacteremia, with escheri ... | 2001 | 11456356 |
| toxin-induced vasogenic cerebral oedema in a rat model. | vasogenic cerebral oedema (vco) was induced in hooded wistar rats by intraperitoneal injection of clostridium perfringens type d epsilon prototoxin. animals were killed, 1 h to 14 d postinjection, by perfusion fixation under general anaesthesia. vco was detected by the presence of endogenous albumin in the brain, visualised by immunocytochemistry. as early as 1 h postinjection, albumin was detected in the walls of cerebral microvessels. maximal diffuse leakage within the neural parenchyma was se ... | 2000 | 11450014 |
| comparative biochemical and immunocytochemical studies reveal differences in the effects of clostridium perfringens enterotoxin on polarized caco-2 cells versus vero cells. | since most in vitro studies exploring the action of clostridium perfringens enterotoxin (cpe) utilize either vero or caco-2 cells, the current study directly compared the cpe responsiveness of those two cell lines. when cpe-treated in suspension, both caco-2 and vero cells formed sds-resistant, cpe-containing complexes of approximately 135, approximately 155, and approximately 200 kda. however, confluent transwell cultures of either cell line cpe-treated for 20 min formed only the approximately ... | 2001 | 11445574 |
| cooling rate effect on outgrowth of clostridium perfringens in cooked, ready-to-eat turkey breast roasts. | the potential for clostridium perfringens spores to germinate and grow in cooked, ready-to-eat turkey products was evaluated to determine a safe cooling rate within the critical temperatures of 48.9 c (120 f) through 12.8 c (55 f). raw turkey deli breast roasts were inoculated with a cocktail of c. perfringens spores (nctc 8238, nctc 8239, and nctc 10388) and cooked in a steam oven to an internal temperature of 72 c. the sample roasts were then cooled through the critical cooling range at rates ... | 2001 | 11441851 |
| clinical features of clostridial bacteremia: a review from a rural area. | blood samples, which were obtained from patients who lived in a rural area with approximately 500 acute-care hospital beds, were cultured from 1990 through 1997. we retrospectively reviewed the blood cultures that yielded clostridium species (74 [0.12%] of 63,296 cultures). these were obtained from 46 different hospitalized patients (incidents per hospital, 0.03%). the source of the clostridium species was a gastrointestinal site in 24 patients (52.2%). the most frequently identified clostridium ... | 2001 | 11438901 |
| characterization and mutagenesis of the recombinant n-acetylneuraminate lyase from clostridium perfringens: insights into the reaction mechanism. | the n-acetylneuraminate lyase from clostridium perfringens was expressed in escherichia coli as a fusion protein with a his-tag and purified to homogeneity using metal chelate affinity and anion exchange chromatography. the purified enzyme has a ph optimum of 7.6 and a temperature optimum of 65-70 degrees c. in kinetic studies the lyase exhibits a km of 3.2 mm for neu5ac and a vmax of 27.5 u x mg(-1). to clarify the functional role of some putative active site residues, site-directed mutagenesis ... | 2001 | 11432751 |
| physical and genetic map of the clostridium saccharobutylicum (formerly clostridium acetobutylicum) ncp 262 chromosome. | a physical and genetic map of the clostridium saccharobutylicum ncp 262 chromosome was constructed. the order of macrorestriction fragments was determined by analysing fragments generated after single and double digestion with the restriction enzymes bsshii, i-ceui, sse8387i, rsrii and sfii and separation by pfge. the i-ceui backbone of c. saccharobutylicum was constructed by indirect end-labelling with rrs- and 3' rrl-specific probes located on either side of the i-ceui site in the rrn operon, ... | 2001 | 11429467 |
| pcr detection and prevalence of alpha-, beta-, beta 2-, epsilon-, iota- and enterotoxin genes in clostridium perfringens isolated from lambs with clostridial dysentery. | clostridium perfringens isolated from lambs with dysentery (n=117) were analysed by a dna amplification technique, the polymerase chain reaction (pcr), in order to determine the prevalence of the alpha-, beta-, beta 2-, epsilon-, iota- and enterotoxin genes. the most prevalent toxin type of c. perfringens found was type b, containing the alpha-, beta-, and epsilon-toxin genes, representing 46% of the cases with clostridial dysentery. c. perfringens type c containing the alpha-, and beta-toxin ge ... | 2001 | 11423193 |
| clostridium perfringens beta toxin and clostridium septicum alpha toxin: their mechanisms and possible role in pathogenesis. | the clostridium septicum alpha toxin and the clostridium perfringens beta toxin are examples of pore-forming toxins that exhibit several different features. the cell types that are targeted by these toxins reflect the effect these toxins have on the host during infection with either organism. alpha toxin elicits a rapid shock-like syndrome, whereas beta toxin appears to induce a variety of neurological effects. the effects of the purified toxins appear to mimic some of the features of the animal ... | 2001 | 11423190 |
| the cholesterol-dependent cytolysins. | in view of the recent studies on the cdcs, a reasonable schematic of the stages leading to membrane insertion of the cdcs can be assembled. as shown in fig. 3, we propose that the cdc first binds to the membrane as a monomer. these monomers then diffuse laterally on the membrane surface to encounter other monomers or incomplete oligomeric complexes. presumably, once the requisite oligomer size is reached, the prepore complex is converted into the pore complex and a large membrane channel is form ... | 2001 | 11417120 |
| the effects of subminimal inhibitory concentrations of beta-lactam antibiotics against clostridium perfringens. | the effects of subminimal inhibitory concentrations (sub-mic) of four beta-lactam antibiotics [penicillin-g (pcg), ampicillin (amp), cephaloridine (cer), cephalothin (cet)] were tested against clostridium perfringens type a pb6k, after determining the minimum inhibitory concentrations (mic) of 29 different clostridium strains. the majority of the strains were sensitive to all beta-lactam antibiotics. morphological changes, such as filamentous development and lysis, occurred at concentrations con ... | 2001 | 11414501 |
| cholangiohepatitis in broiler chickens in japan: histopathological, immunohistochemical and microbiological studies of spontaneous disease. | forty-five broiler carcasses from 6 different flocks were condemned due to liver lesions at processing meat inspection, and collected for pathological and bacterial examinations. all affected chickens showed liver enlargement with discolouration and an apparent acinar pattern. the enlarged gallbladder and the extrahepatic bile ducts contained yellow inspissated cream-coloured material. histopathologically, extensive proliferation of bile ductules with fibrosis was observed in interlobular connec ... | 2000 | 11402676 |
| acute necrotizing bacterial tonsillitis with clostridium perfringens. | bacterial infection with clostridium perfringens in children less than 2 years of age is frequently associated with meningitis, necrotizing gastrointestinal infection, and postoperative infections. however, a review of the literature reveals no reports of these bacteria infecting the tonsils. a 9-month old black female was found unresponsive at the baby-sitter's and was rushed to the hospital. shortly after admission to the emergency department death was pronounced. an autopsy performed on this ... | 2001 | 11394754 |
| clostridium perfringens as a potential indicator for the presence of sewage solids in marine sediments. | marine sediment cores collected from several depths of water and distances from a california sewage outfall were tested to see if sediments influenced by sewage solids were a reservoir of enteric pathogens, and if concentrations of indicator bacteria were related to the presence of sewage solids. vertical distributions of microorganisms in marine sediments were determined; there was a decrease of indicator bacteria with increasing sediment depth. aeromonas was randomly isolated, but none of the ... | 2001 | 11382981 |
| a novel endo-beta-galactosidase from clostridium perfringens that liberates the disaccharide glcnacalpha 1-->gal from glycans specifically expressed in the gastric gland mucous cell-type mucin. | we found that commercially available sialidases prepared from clostridium perfringens atcc10543 were contaminated with an endoglycosidase capable of releasing the disaccharide glcnacalpha1-->4gal from glycans expressed in the gastric gland mucous cell-type mucin. we have isolated this enzyme in electrophoretically homogeneous form from the culture supernatant of this organism by ammonium sulfate precipitation followed by affinity chromatography using a sephacryl s-200 hr column. the enzyme was s ... | 2001 | 11382776 |
| assessment and impact of microbial fecal pollution and human enteric pathogens in a coastal community. | the goals of this study were to assess watersheds impacted by high densities of osds (onsite sewage disposal systems) for evidence of fecal contamination and evaluate the occurrence of human pathogens in coastal waters off west florida. eleven stations (representing six watersheds) were intensively sampled for microbial indicators of fecal pollution (fecal coliform bacteria, enterococci, clostridium perfringens and coliphage) and the human enteric pathogens, cryptosporidium, giardia, and enterov ... | 2001 | 11381749 |
| differentiation of clostridium perfringens and clostridium botulinum from non-toxigenic clostridia, isolated from prepared and frozen foods by pcr-dan based methods. | during the elaboration process of prepared and frozen foods, clostridium sp. have been reported. from those microorganisms, c. perfringens and c. botulinum may pose a high risk for the consumers. to avoid these pathogenic organisms an haccp program should be implemented, but in addition sensitive and moderately time consuming microbiological methods for monitoring c. perfringens and c. boulinum should be established. in this work, an rflp analysis of the 16s rdna will be developed to differentia ... | 2001 | 11379285 |
| molecular evidence that the capacity for endosporulation is universal among phototrophic heliobacteria. | although enrichment cultures for anoxygenic phototrophic heliobacteria commonly contain sporulating cells, once strains of heliobacteria are obtained in pure culture, they all but cease to sporulate. in fact, some species of heliobacteria have never been observed to sporulate. thus, despite their phylogenetic connection to endospore-forming bacteria, the question of sporulation capacity in heliobacteria remains open. we have investigated this problem using pcr and southern hybridization as tools ... | 2001 | 11377866 |
| partial characterization of an enzyme fraction with protease activity which converts the spore peptidoglycan hydrolase (slec) precursor to an active enzyme during germination of clostridium perfringens s40 spores and analysis of a gene cluster involved in the activity. | a spore cortex-lytic enzyme of clostridium perfringens s40 which is encoded by slec is synthesized at an early stage of sporulation as a precursor consisting of four domains. after cleavage of an n-terminal presequence and a c-terminal prosequence during spore maturation, inactive proenzyme is converted to active enzyme by processing of an n-terminal prosequence with germination-specific protease (gsp) during germination. the present study was undertaken to characterize gsp. in the presence of 3 ... | 2001 | 11371539 |
| intestinal epithelial damage in sids babies and its similarity to that caused by bacterial toxins in the rabbit. | sections of the duodenum, jejunum, ileum, caecum and large intestine from 14 sudden infant death syndrome (sids) babies were examined by scanning (sem) and transmission electron microscopy (tem). the type and amount of damage was characterised and quantitated and compared with the presence of clostridium perfringens, clostridium difficile, escherichia coli and staphylococcus aureus in faecal samples from the babies and toxins from the bacteria in faecal samples and serum from the babies. the dat ... | 2001 | 11358053 |
| the effect of bacterial enterotoxins implicated in sids on the rabbit intestine. | the aim of this project was to characterise the type of damage caused to the intestine of the infant rabbit by bacterial enterotoxins implicated in sudden infant death syndrome (sids). samples of the duodenum, jejunum, ileum, caecum and large intestine exposed to the toxins for up to 6 hours were examined by scanning (sem) and transmission electron microscopy (tem). the damage was quantitatively assessed (% villi damaged) by sem and qualitatively by sem and tem. clostridium perfringens enterotox ... | 2001 | 11358052 |
| role of residues constituting the 2beta1 strand of domain ii in the biological activity of anthrax protective antigen. | anthrax toxin consists of three proteins, protective antigen, lethal factor and oedema factor. a proteolytically activated 63-kda fragment of protective antigen binds lethal factor/oedema factor and translocates them into the cytosol. domain ii of protective antigen has been implicated in membrane insertion and channel formation. in the present study, alanine substitutions in 14 consecutive residues of the 2beta1 strand that are highly homologous to the putative membrane interacting segment of c ... | 2001 | 11356563 |
| bacterial intestinal flora associated with enterotoxaemia in belgian blue calves. | the enterotoxaemia syndrome in belgian blue calves is characterised by a high case fatality rate, sudden death, lesions of haemorrhagic enteritis of the small intestine and, quite often an absence of other clinical signs but its cause has not been yet identified. as a first step in this identification, the aerobic and anaerobic intestinal flora of a population of 78 calves, originating from farms located in southern belgium and that died in circumstances defined as "calf enterotoxaemia" (study p ... | 2001 | 11356315 |
| weakly beta-haemolytic human intestinal spirochaetes antagonize the haemolytic activity of clostridium perfringens alpha-toxin producer. | the production of haemolytic antagonism between weakly beta-haemolytic human intestinal spirochaetes (wbetahis) related to human intestinal spirochaetosis and clostridium perfringens alpha-toxin producer was investigated. a reduction of the clostridial haemolytic activity and a distortion of the haemolytic halo of clostridial alpha-toxin surrounded by a small zone of poorly cooperative haemolysis was clearly observed on the level of the spirochaetal growth area when 40 out of 41 wbetahis were cu ... | 2001 | 11346295 |
| seasonal variations in the microbial population density present in biological sludge. | sludge produced during the treatment of wastewater is being used as fertilizer in several gulf countries. the water and sewage authority of saudi arabia has targeted the reuse of the total amount of sludge in the future. however, these sludges should be properly treated before reuse as they contain a large number of pathogens and parasites. little information is available on the microbial characteristics of sludge produced in wastewater treatment plants operating in this region. variations in th ... | 2001 | 11346282 |
| microbiological status of commercially available medicinal herbal drugs--a screening study. | one hundred and thirty-eight medicinal herbal drugs obtained from different suppliers were examined for microbial contaminants and for the detectability of pathogenic microorganisms. for this purpose, several microbiological standard parameters (total aerobic mesophilic count, enterobacteria, coliforms, aerobic sporeformers, yeasts and moulds, enterococci, lactobacilli, pseudomonades and aeromades) and selective methods for the detection of indicator microorganisms and pathogens (e. coli, entero ... | 2001 | 11345700 |
| tyrosine 331 and phenylalanine 334 in clostridium perfringens alpha-toxin are essential for cytotoxic activity. | differences in the biological properties of the clostridium perfringens phospholipase c (alpha-toxin) and the c. bifermentans phospholipase c (cbp) have been attributed to differences in their carboxy-terminal domains. three residues in the carboxy-terminal domain of alpha-toxin, which have been proposed to play a role in membrane recognition (d269, y331 and f334), are not conserved in cbp (y, l and i respectively). we have characterised d269y, y331l and f334i variant forms of alpha-toxin. varia ... | 2001 | 11334886 |
| efficacy of narasin in the prevention of necrotic enteritis in broiler chickens. | the efficacy of narasin in the control of necrotic enteritis (ne) was investigated in a floor pen study of 2000 broiler chickens using a clostridium perfringens feed inoculum challenge model. treatments were 1) nonmedicated, nonchallenged; 2) nonmedicated, challenged; 3) narasin, nonchallenged; 4) narasin, challenged. narasin was administered at 70 ppm in the feed from day 0 to trial termination on day 41. challenge inoculum contained approximately 1 x 10(8) colony-forming units cp/ml and was ad ... | 2001 | 11332485 |
| reduced incidence of clostridium perfringens-associated lesions and improved performance in broiler chickens treated with normal intestinal bacteria from adult fowl. | the dosing of young chicks with cultures of normal gut flora has been termed "competitive exclusion" (ce). this study was undertaken to examine, under field conditions, the effect of ce treatment on counts of intestinal clostridium perfringens (cp) and on the occurrence of cp-associated disease in broiler chickens. a farm having recurrent cp-associated health problems was selected as study site. the study comprised four broiler houses, with one treated and one untreated flock per house. treated ... | 2001 | 11332476 |
| case of the season. emphysematous cholecystitis. | 2001 | 11329659 | |
| recurrent diarrhea associated with enterotoxigenic clostridium perfringens in 2 dogs. | two dogs were diagnosed with enterotoxigenic clostridium perfringens-associated diarrhea. diarrhea was responsive to antimicrobial therapy, but recurred after treatment was ceased. clostridium perfringens enterotoxin was present in feces during diarrheic episodes but not when feces were normal. both dogs responded to a prolonged course of oral cephalexin and dietary modification. | 2001 | 11326633 |
| linezolid: a review of its use in the management of serious gram-positive infections. | linezolid is the first of a new class of antibacterial drugs, the oxazolidinones. it has inhibitory activity against a broad range of gram-positive bacteria, including methicillin-resistant staphylococcus aureus (mrsa), glycopeptide-intermediate s. aureus (gisa), vancomycin-resistant enterococci (vre) and penicillin-resistant streptococcus pneumoniae. the drug also shows activity against certain anaerobes, including clostridium perfringens, c. difficile, peptostreptococcus spp. and bacteroidesfr ... | 2001 | 11324682 |
| foodborne diseases in kenya. | to determine the occurrence of foodborne disease outbreaks in kenya and the efforts employed to combat them. | 2001 | 11320765 |
| comparison of tn5397 from clostridium difficile, tn916 from enterococcus faecalis and the cw459tet(m) element from clostridium perfringens shows that they have similar conjugation regions but different insertion and excision modules. | comparative analysis of the conjugative transposons tn5397 from clostridium difficile and tn916 from enterococcus faecalis, and the cw459tet(m) element from clostridium perfringens, has revealed that these tetracycline-resistance elements are closely related. all three elements contain the tet(m) resistance gene and have sequence similarity throughout their central region. however, they have very different integration/excision modules. instead of the int and xis genes that are found in tn916, tn ... | 2001 | 11320127 |
| evaluation of an automated culture system for detecting bacterial contamination of platelets: an analysis with 15 contaminating organisms. | approximately 1 in 2000 platelet components are bacterially contaminated. the time to detection of 15 seeded organisms in platelets recovered from an automated culture system was studied. | 2001 | 11316897 |
| removal of indicator bacteria, human enteric viruses, giardia cysts, and cryptosporidium oocysts at a large wastewater primary treatment facility. | pathogens and fecal indicator bacteria occurrence and removal were studied for a period of 6 months at the montreal urban community wastewater treatment facility. with a capacity of about 7.6 million cubic metres per day (two billion u.s. gallons per day), it is the largest primary physico-chemical treatment plant in america. the plant discharges a nondisinfected effluent containing about 20 mg/l of suspended matter and 0.5 mg/l of total phosphorus on the basis of average annual concentrations. ... | 2001 | 11315109 |
| clostridium perfringens prototoxin-induced alteration of endothelial barrier antigen (eba) immunoreactivity at the blood-brain barrier (bbb). | it has been reported that the severe cerebral edema produced in experimental animals by clostridium perfringens (cl p) type d epsilon toxin can be prevented by prior treatment with its precursor prototoxin due to competitive binding to endothelial cells (ecs) at the blood-brain barrier (bbb). in this study we investigate the effects of the prototoxin on the bbb, without added toxin. the integrity of the bbb was assessed by its ability to prevent leakage of endogenous albumin. ecs at the bbb were ... | 2001 | 11312560 |
| selective binding of perfringolysin o derivative to cholesterol-rich membrane microdomains (rafts). | there is increasing evidence that sphingolipid- and cholesterol-rich microdomains (rafts) exist in the plasma membrane. specific proteins assemble in these membrane domains and play a role in signal transduction and many other cellular events. cholesterol depletion causes disassembly of the raft-associated proteins, suggesting an essential role of cholesterol in the structural maintenance and function of rafts. however, no tool has been available for the detection and monitoring of raft choleste ... | 2001 | 11309501 |
| elucidation of the role of functional amino acid residues of the small sialidase from clostridium perfringens by site-directed mutagenesis. | bacterial sialidases represent important colonization or virulence factors. the development of a rational basis for the design of antimicrobials targeted to sialidases requires the knowledge of the exact roles of their conserved amino acids. a recombinant enzyme of the 'small' (43 kda) sialidase of clostridium perfringens was used as a model in our study. several conserved amino acids, identified by alignment of known sialidase sequences, were altered by site-directed mutagenesis. all recombinan ... | 2001 | 11308029 |
| infections in patients with immunodeficiency with thymoma (good syndrome). report of 5 cases and review of the literature. | immunodeficiency with thymoma (good syndrome, gs) is a rare, adult-onset condition that is characterized by thymoma, hypogammaglobulinemia, and low numbers of peripheral b cells. cd4+ t lymphopenia and an inverted cd4:cd8+ t-cell ratio may be present. here we report 5 patients with gs and infectious complications who were seen at 3 institutions between 1983 and 1999. three patients had recurrent sinopulmonary infections, 3 had severe cytomegalovirus (cmv) disease, and 1 had pneumocystis carinii ... | 2001 | 11307588 |
| bactericidal properties of the chloroform fraction from rhizomes of aristolochia paucinervis pomel. | the deffated chloroform fraction (aprc) obtained from the rhizomes of aristolochia paucinervis pomel (aristolochiaceae) has a high bacteriostatic activity against bacterial strains like clostridium perfringens atcc 13124 and enterococcus faecalis atcc 29212. here, we report the bactericidal activity of aprc against both strains which was evaluated by using time-to kill assays. the results showed that aprc produced an intense time-dependent bactericidal effect against c. perfringens, achieving ov ... | 2001 | 11297853 |
| genomic map of clostridium perfringens strain 13. | a physical and genetic map of clostridium perfringens strain 13 was constructed. c. perfringens strain 13 was found to have a 3.1-mb chromosome and a large 50-kb plasmid, indicating that strain 13 has a relatively small genome among c. perfringens strains. a total of 313 genetic markers were mapped on the chromosome of strain 13. compared with the physical and genetic map of c. perfringens cpn50, strain 13 had a quite similar genome organization, but with a large deletion (approximately 400 kb) ... | 2001 | 11293486 |
| enterotoxin plasmid from clostridium perfringens is conjugative. | clostridium perfringens enterotoxin is the major virulence factor involved in the pathogenesis of c. perfringens type a food poisoning and several non-food-borne human gastrointestinal illnesses. the enterotoxin gene, cpe, is located on the chromosome of food-poisoning isolates but is found on a large plasmid in non-food-borne gastrointestinal disease isolates and in veterinary isolates. to evaluate whether the cpe plasmid encodes its own conjugative transfer, a c. perfringens strain carrying pm ... | 2001 | 11292780 |
| cellular uptake of the clostridium perfringens binary iota-toxin. | the binary iota-toxin is produced by clostridium perfringens type e strains and consists of two separate proteins, the binding component iota b (98 kda) and an actin-adp-ribosylating enzyme component iota a (47 kda). iota b binds to the cell surface receptor and mediates the translocation of iota a into the cytosol. here we studied the cellular uptake of iota-toxin into vero cells. bafilomycin a1, but not brefeldin a or nocodazole, inhibited the cytotoxic effects of iota-toxin, indicating that t ... | 2001 | 11292715 |
| hyperbaric oxygen therapy in acute necrotizing infections. with a special reference to the effects on tissue gas tensions. | clostridial gas gangrene and perineal necrotizing fasciitis or fournier's gangrene are rare but serious infections with an acute onset, rapid progression, systemic toxemia and a high mortality rate. the aim of this study was to investigate the efficacy of surgery, antibiotic treatment, surgical intensive care and in particular the role of hyperbaric oxygen (hbo) in the management of these infections. an experimental rat model was used to investigate the possibilities for measuring tissue oxygen ... | 2000 | 11288993 |
| a survey of ethnic foods for microbial quality and aflatoxin content. | a range of ethnic foods was examined for their microbiological content in relation to total viable counts (tvc) of aerobic bacteria, counts of presumptive coliforms, yeast and mould counts; presence of salmonella spp., listeria monocytogenes, escherichia coli o157:h7 and campylobacter spp.; total enumeration of clostridium perfringens, staphylococcus aureus and bacillus spp.; identification of moulds and the presence of total aflatoxins. samples, which included cereals, nuts, dried fruits, herbs ... | 2001 | 11288910 |
| [intestinal flora of patients with suspected antibiotic associated diarrhea (aad). i. clostridium perfringens]. | stool samples of 158 patients suspected of antibiotic-associated diarrhoea (aad) were studied. toxin a of c. difficile and enterotoxin of c. perfringens were detected in stool samples by immunoenzymatic assays and pcr. in 35 stool samples toxin a of c. difficile was detected and in 48 cases (30%) c. difficile strains were cultured from 21 stool samples (13%). the presence of the cpe gene of c. perfringens, enabling the production of enterotoxin, could not be detected by pcr, both in stool sample ... | 2000 | 11286179 |
| detection of phospholipase c in nontuberculous mycobacteria and its possible role in hemolytic activity. | phospholipase c plays a key role in the pathogenesis of several bacterial infections, for example, those caused by clostridium perfringens and listeria monocytogenes. previous studies have reported multiple copies of plc genes homologous to pseudomonas aeruginosa plch and plcn genes encoding the hemolytic and nonhemolytic phospholipase c enzymes in the genomes of mycobacterium tuberculosis, m. marinum, m. bovis, and m. ulcerans. in this study we analyzed the possible relationship between phospho ... | 2001 | 11283062 |
| [collaboration between laboratories with regard to instances of food poisoning]. | 2001 | 11282532 | |
| cleavage of a c-terminal peptide is essential for heptamerization of clostridium perfringens epsilon-toxin in the synaptosomal membrane. | activation of clostridium perfringens epsilon-protoxin by tryptic digestion is accompanied by removal of the 13 n-terminal and 22 c-terminal amino acid residues. in this study, we examined the toxicity of four constructs: an epsilon-protoxin derivative (pd), in which a factor xa cleavage site was generated at the c-terminal trypsin-sensitive site; pd without the 13 n-terminal residues (deltan-pd); pd without the 23 c-terminal residues (deltac-pd); and pd without either the n- or c-terminal resid ... | 2001 | 11278924 |