Publications

characterization of the structural proteins of hemorrhagic enteritis virus.	the structural proteins of hemorrhagic enteritis (hev), a turkey adenovirus, were analyzed by polyacrylamide gel electrophoresis (page) and western blotting using polyspecific, monospecific and monoclonal antibodies for detection. in purified hev preparations, eleven polypeptides with apparent molecular weights ranging from 96,000 to 9,500 (96k to 9.5k), were specifically recognized by convalescent turkey serum. six of these polypeptides were further characterized by page, western blotting, elis ...	1992	1326260
increased numbers of duodenal mucosal mast cells in turkeys inoculated with hemorrhagic enteritis virus.	the relation between average duodenal mast cell count, duodenal mucosal mast cell numbers, duodenal connective tissue mast cell numbers, circulating basophil numbers, heterophil-to-lymphocyte ratio, and lesion score were studied to gain an understanding of the events that may lead to intestinal lesion formation associated with hemorrhagic enteritis virus (hev) infection. changes in vascular permeability in the duodenum in birds inoculated with hev were examined, using colloidal carbon and ferrit ...	1992	1326244
recent developments in the molecular cloning and characterization of hepatitis c and e viruses.	the molecular properties of the genomes of both hepatitis c virus (hcv) and hepatitis e virus (hev), the major etiologic agents of non-a, non-b hepatitis, are briefly described. the organization of the genome of each of these viruses is discussed and compared to those of other related or distantly related viruses that contain single-stranded, positive-sense rna genomes. in the case of hev, the reactivity of expressed proteins and genetic divergence of geographically distinct isolates are also de ...	1992	1326075
relationship between age of flock seroconversion to hemorrhagic enteritis virus and appearance of adenoviral inclusions in the spleen and renal tubule epithelia of turkeys.	a retrospective study was conducted to evaluate the temporal relationship between flock seroconversion to hemorrhagic enteritis virus (hev) and the appearance of adenoviral inclusions in the spleen and renal tubular epithelium. the study was conducted on samples of turkey poults submitted to the fresno branch of the california veterinary diagnostic laboratory system during may to december 1988. the study included 78 submissions (four to eight poults per submission) of ages ranging from 6 to 15 w ...	1992	1314556
[immunohistochemical study of hepatitis e virus antigen in liver tissues from patients with type e hepatitis].	hepatitis e virus antigen (hev ag) has been identified in liver tissues of 6 out of 14 patients with type e hepatitis by direct immunoperoxidase staining, using anti-hev-igg-hrp. hev ag was found to be diffusive or inclusive in the cytoplasm of hepatocytes were scattered and isolated, but might aggregate in some parts where the liver damage was severe. we found that lymphocyte invaded the hev ag positive hepatocyte. these phenomena suggest that the liver damage is related to immunoreaction. hev ...	1992	1303848
[hepatitis e virus].		1992	1292604
haemorrhagic disease of lagomorphs: evidence for a calicivirus.	studies on the aetiological agents of rabbit haemorrhagic disease (rhd) and european brown hare syndrome show that the viruses responsible for these infections can be placed in the family caliciviridae. established members of this group are vesicular exanthema virus (prototype), san miguel sea lion virus and feline calcivirus. the human hepatitis e virus and the norwalk agent may soon be included. the rhd virus genome consists of a positive stranded rna molecule composed of 7437 nucleotides. a m ...	1992	1282757
detection of norwalk virus in stool specimens by reverse transcriptase-polymerase chain reaction and nonradioactive oligoprobes.	a reverse transcriptase (rt)-polymerase chain reaction (pcr)-oligoprobe (op), or rt-pcr-op, method was developed for the detection of the norwalk virus, which causes acute, epidemic gastroenteritis, in stool specimens. the norwalk virus genome regions encoding the following two proteins were amplified by rt-pcr: the rna polymerase (260-bp product) and a putative immunogenic protein (224-bp product). the resulting dna fragments (amplicons) were hybridized to a digoxigenin-labeled internal op spec ...	1992	1280649
[hemagglutinating encephalomyelitis virus (hev) infections in swine. serologic indication for infectional occurrence in denmark].	in 1956 a disease of nursing pigs previously unreported in denmark, was observed in several swine herds. the disease syndrome appeared identical with that later described by roe and alexander in 1958 and by s. f. cartwright and m. lucas in 1968. since 1956 there have been no reports of such outbreaks in danish herds. to investigate whether the virus is still present, a survey was carried out to detect neutralizing antibodies in sera from a number of boars using a micro technique. in 144 sera fro ...	1975	1144016
incidence of antibody for hemagglutinating encephalomyelitis virus in serums from swine in the united states.	serums prepared from blood collected from swine in arizona, florida, georgia, hawaii, iowa, maine, new hampshire, oregon, vermont, and washington were examined by the hemagglutination-inhibition (hi) test for antibody to hemagglutinating encephalomyelitis virus (hev). antibody was detected in serums from swine in each of the states except maine, new hampshire, and vermont. the frequency of antibody ranged from 10.9% (6 of 55) with serums from swine in oregon, to 98.6% (71 of 72) with serums from ...	1975	807134
effect of sulphydryl reagents on the biological activities, polypeptide composition and morphology of haemagglutinating encephalomyelitis virus.	the effect of sulphydryl reagents on haemagglutinating encephalomyelitis virus (hev), a coronavirus of pigs, was investigated. using increasing concentrations of dithiothreitol (dtt), 50% of the virus infectivity and haemagglutination (ha) activity could be removed by i.5 mm and 4 to 5 mm respectively. the effect of dtt concentrations on the polypeptide composition was also examined. of the three external glycoproteins gp 125 was found to be the most susceptible, 50% being removed by incubation ...	1978	567674
a seroepizootiologic study of five viruses in a swine-evaluation station.	this serologic study was done to gain information on the spread, maintenance, and effect upon performance of five porcine viruses. blood samples were taken from two groups of 8- to 11-week-old pigs from a large number of indiana swine herds in a performance-testing station 1 week after entry, 7 weeks after entry (one group only), and at slaughter. the sera were tested by indirect fluorescent antibody tests for antibodies to transmissible gastroenteritis virus (tgev), swine influenza virus (siv), ...	1979	230762
experimental infection of chickens with hemorrhagic enteritis virus.	chickens were experimentally infected with hemorrhagic enteritis virus, causing lesions similar to those observed in turkeys. lesions were induced by intraperitoneal and oral infection. virus particles with the morphology of adenovirus were demonstrated in the spleens of infected chickens. splenic extracts from infected chickens caused lesions of hemorrhagic enteritis in turkeys. recovery from the infection in chickens was followed by the production of antibodies to hev, demonstrated by agar-gel ...	1978	206247
an electron microscopic study of experimentally-induced hev encephalitis.	a gnobiotic piglet, was inoculated intracerebrally with hemagglutinating encephalomyelitis virus (strain vw572). mononuclear cells formed vascular cuffs and were disseminated in the brain parenchyma. a few neurons were surrounded by the same kind of cells. virus particles morphologically similar to coronavirus particles were found in the cytoplasm of both chromatolytic light neurons and hyperchromic dark neurons. the particles were in vesicles of distended endoplasmic reticulum and in the hypert ...	1978	204096
porcine parvovirus: frequency of naturally occurring transplacental infection and viral contamination of fetal porcine kidney cell cultures.	the frequency of naturally occurring transplacental infection of swine with porcine parvovirus (ppv) and one of the possible consequences of such infection--the presence of ppv in cell cultures prepared from fetal tissues--were investigated. transplacental infection was indicated by the presence of high titers of hemagglutination inhibiting (hi) antibody for ppv in serums of 0-day-old, hysterectomy-derived, colostrum-deprived pigs of 3 of 82 litters. all letters were farm-raised dams. moreover, ...	1975	163603
antigenic relationship of the feline infections peritonitis virus to coronaviruses of other species.	utilizing the direct and indirect fluorescent antibody procedure, the antigenic relationship of the feline infectious peritonitis virus (fipv) to 7 other human and animal coronaviruses was studied. fipv was found to be closely related to transmissible gastroenteritis virus (tgev) of swine. transmissible gastroenteritis virus and fipv were in turn antigenically related to human coronavirus 229e (hcv-229e) and canine coronavirus (ccv). an interesting finding in the study was that the 8 coronavirus ...	1978	81044
antigenic relationship between human coronavirus strain oc 43 and hemagglutinating encephalomyelitis virus strain 67n of swine: antibody responses in human and animal sera.	hemagglutinating encephalomyelitis virus of swine (hev) was adapted to growth in suckling mouse brain. electron micrographs of hev-infected suckling mouse brain, prepared by negative staining and thin-section techniques, exhibited typical morphological characteristics shared with other members of the coronaviridae. the adaptation of hev to suckling mouse brain facilitated serologic testing by the use of common host reagents and compatible animal systems. with hemagglutination inhibition, complem ...	1977	65439