Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| plasmacytoid dendritic cells promote host defense against acute pneumovirus infection via the tlr7-myd88-dependent signaling pathway. | human respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract infection in infants. in human infants, plasmacytoid dendritic cells (pdc) are recruited to the nasal compartment during infection and initiate host defense through the secretion of type i ifn, il-12, and il-6. however, rsv-infected pdc are refractory to tlr7-mediated activation. in this study, we used the rodent-specific pathogen, pneumonia virus of mice (pvm), to determine the contribution of pdc and tlr7 s ... | 2011 | 21482736 |
| expression analysis of the nucleocytoplasmic lectin 'orysata' from rice in pichia pastoris. | the oryza sativa lectin, abbreviated orysata, is a mannose-specific, jacalin-related lectin expressed in rice plants after exposure to certain stress conditions. expression of a fusion construct containing the rice lectin sequence linked to enhanced green fluorescent protein in bright yellow 2 tobacco cells revealed that orysata is located in the nucleus and the cytoplasm of the plant cell, indicating that it belongs to the class of nucleocytoplasmic jacalin-related lectins. since the expression ... | 2011 | 21481190 |
| safety profile of decellularized, cryopreserved pulmonary allografts when used in the aortic position for neonatal arch reconstruction. | cryopreserved pulmonary allograft is frequently used to reconstruct the hypoplastic aortic arch. a decellularized graft preparation is available as an alternative reconstructive material. this case series profiles our experience using this material for neonatal aortic arch reconstruction. data from 14 patients who received decellularized pulmonary allograft (dcpa) from 2001 to 2003 included the following: diagnosis; age at implantation; perioperative complications (graft bleeding or dehiscence); ... | 2011 | 21479906 |
| double-stranded rna induces similar pulmonary dysfunction to respiratory syncytial virus in balb/c mice. | both respiratory syncytial virus (rsv) and influenza a virus induce nucleotide/p2y purinergic receptor-mediated impairment of alveolar fluid clearance (afc), which contributes to formation of lung edema. although genetically dissimilar, both viruses generate double-stranded rna replication intermediates, which act as toll-like receptor (tlr)-3 ligands. we hypothesized that double-stranded rna/tlr-3 signaling underlies nucleotide-mediated inhibition of amiloride-sensitive afc in both infections. ... | 2011 | 21478252 |
| cost-effectiveness analysis of palivizumab as respiratory syncytial virus prophylaxis in preterm infants in sweden. | aim: to investigate the cost-effectiveness of palivizumab vs. no prophylaxis for respiratory syncytial virus (rsv) infection in preterm infants in sweden. methods: a probabilistic markov model was populated using a nationwide register linkage and data from the literature. cost-effectiveness was investigated from a societal perspective over a lifetime for infants born at <29 weeks of gestation. palivizumab was modelled using assumptions for its direct effect on rsv hospitalization risk and an i ... | 2011 | 21477089 |
| modeling competing infectious pathogens from a bayesian perspective: application to influenza studies with incomplete laboratory results. | in seasonal influenza epidemics, pathogens such as respiratory syncytial virus (rsv) often co-circulate with influenza and cause influenza-like illness (ili) in human hosts. however, it is often impractical to test for each potential pathogen or to collect specimens for each observed ili episode, making inference about influenza transmission difficult. in the setting of infectious diseases, missing outcomes impose a particular challenge because of the dependence among individuals. we propose a b ... | 2010 | 21472041 |
| differential pathogenesis of respiratory syncytial virus clinical isolates in balb/c mice. | airway mucus is a hallmark of respiratory syncytial virus (rsv) lower respiratory tract illness. laboratory rsv strains differentially induce airway mucus production in mice. here, we tested the hypothesis that rsv strains differ in pathogenesis by screening six low-passage rsv clinical isolates for mucogenicity and virulence in balb/cj mice. the rsv clinical isolates induced variable disease severity, lung interleukin-13 (il-13) levels, and gob-5 levels in balb/cj mice. we chose two of these cl ... | 2011 | 21471228 |
| viral-mediated inhibition of antioxidant enzymes contributes to the pathogenesis of severe respiratory syncytial virus bronchiolitis. | rationale: respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infections in children, for which no specific treatment or vaccine is currently available. we have previously shown that rsv induces reactive oxygen species in cultured cells and oxidative injury in the lungs of experimentally infected mice. the mechanism(s) of rsv-induced oxidative stress in vivo is not known. objectives: to measure changes of lung antioxidant enzymes expression/activity and activation of n ... | 2011 | 21471094 |
| detection of microorganisms in exhaled breath condensate during acute exacerbations of copd. | summary at a glance: exhaled breath condensate (ebc) is a noninvasive method to assess airway biology. the aim of the present study was to evaluate ebc for the detection of microbial nucleic acids during acute exacerbations of copd (aecopd). bacterial nucleic acids can be identified in ebcs of copd patients with exacerbations, however, the results differed significantly from those of sputum. abstract: background and objective: one hallmark of chronic obstructive pulmonary disease (copd) is colo ... | 2011 | 21470340 |
| detection of human metapneumovirus in infants with acute respiratory tract infection. | both respiratory syncytial virus (rsv) and human metapneumovirus (hmpv), a recently discovered respiratory pathogen classified in the paramyxoviridae family, have been associated with acute respiratory tract infections (arti) in infants. the aim of our study was to determine the incidence and clinical features of hmpv infection in infants presenting with arti at a tertiary children's hospital in greece during the 2007-2008 and 2008-2009 winter seasons. routine nasal swabs were obtained from 127 ... | 2011 | 21468562 |
| acute lower respiratory tract infection due to respiratory syncytial virus in a group of egyptian children under 5 years of age. | respiratory syncytial virus (rsv) is one of the most important causes of acute lower respiratory tract infections (alrti) in infants and young children. this study was conducted to describe the epidemiology of alrti associated with rsv among children = 5 years old in egypt. | 2011 | 21466713 |
| sheng-ma-ge-gen-tang (shoma-kakkon-to) inhibited cytopathic effect of human respiratory syncytial virus in cell lines of human respiratory tract. | sheng-ma-ge-gen-tang (smggt; shoma-kakkon-to) has been used against pediatric viral infection for thousands of year in ancient china. however, it is unknown whether smggt is effective against human respiratory syncytial virus (hrsv). | 2011 | 21463671 |
| including viral infection data supports an association between particulate pollution and respiratory admissions. | to refine and revise previous air pollution, climate and health time series analysis in christchurch, new zealand, introducing viral identification data (positive identification count and outbreak, defined as two of more positive tests). | 2011 | 21463414 |
| human respiratory syncytial virus in children hospitalized for acute lower respiratory infection. | to evaluate the prevalence and seasonality of human respiratory syncytial virus (hrsv) in children aged 0 to 6 years, hospitalized with acute lower respiratory infection (alri) in são josé do rio preto, sp, brazil, and the association between age, diagnosis, and hrsv. | 2011 | 21461452 |
| dexamethasone in children mechanically ventilated for lower respiratory tract infection caused by respiratory syncytial virus: a randomized controlled trial. | to determine the efficacy of dexamethasone in the treatment of mechanically ventilated children with respiratory syncytial virus-severe lower respiratory tract infection. | 2011 | 21460709 |
| viral infections of the lower respiratory tract: old viruses, new viruses, and the role of diagnosis. | viral infections of the lower respiratory tract cause an enormous disease burden in children, and the role of respiratory viruses in serious lower respiratory tract infections (lrtis) in older adults is increasingly appreciated. although viruses are responsible for a large proportion lrtis, antibiotics are often prescribed. new diagnostic platforms have the potential to detect a wider range of established and newly discovered viruses with greater sensitivity. this will create additional challeng ... | 2011 | 21460286 |
| evaluation of the calu-3 cell line as a model of in vitro respiratory syncytial virus infection. | respiratory syncytial virus (rsv) replication is primarily limited to the upper respiratory tract epithelium and primary, differentiated normal human bronchial epithelial cells (nhbe) have, therefore, been considered a good system for in vitro analysis of lung tissue response to respiratory virus infection and virus-host interactions. however, nhbe cells are expensive, difficult to culture, and vary with the source patient. an alternate approach is to use a continuous cell line that has features ... | 2011 | 21458491 |
| neurological complications of extracorporeal membrane oxygenation in children. | extracorporeal membrane oxygenation (ecmo) is a potentially life-saving treatment for patients in refractory cardiorespiratory failure. neurological complications that result from ecmo treatment are known to significantly impact patient survival and quality of life. the purpose of this study was to review the incidence of neurological complications of ecmo in the pediatric population and the role of neurosurgery in the treatment of these patients. | 2011 | 21456903 |
| viral etiologies of acute respiratory infections among hospitalized vietnamese children in ho chi minh city, 2004-2008. | the dominant viral etiologies responsible for acute respiratory infections (aris) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by aris are highest. improved etiological insight is needed to improve clinical management and prevention. | 2011 | 21455313 |
| [acute bronchiolitis: diagnosis and management]. | acute bronchiolitis is a viral infection of the lower respiratory tract. the infection is frequent among young children and is most commonly caused by the respiratory syncytial virus. the infection causes inflammation and narrowing of the bronchioles which leads to obstructive breathing and respiratory difficulties. the diagnosis is primarily made by clinical examination; laboratory and radiological studies are of little value. treatment is principally supportive and symptomatic. the prognosis i ... | 2011 | 21451194 |
| detection of respiratory viruses by pcr assay of nasopharyngeal swabs stored in skim milk-tryptone-glucose-glycerol transport medium. | we analyzed 129 paired nasopharyngeal aspirates (stored in viral transport medium [vtm]) and nasopharyngeal swabs (stored in skim milk-tryptone-glucose-glycerol [stgg] bacterial transport and storage medium) using pcrs to detect adenoviruses, influenza virus a or b, and respiratory syncytial virus (rsv). overall, swabs stored in stgg medium without antimicrobials were found to be an acceptable alternative to aspirates stored in antimicrobial-containing vtm, with pcr agreement of 90.2% (kappa of ... | 2011 | 21450959 |
| comparison of human metapneumovirus and respiratory syncytial virus in children admitted to a paediatric intensive care unit. | aim: to describe the clinical presentation and course of children admitted to the paediatric intensive care unit (picu) with human metapneumovirus (hmpv) infection, and compare them with children admitted to the picu with respiratory syncytial virus (rsv) infection. methods: hmpv was identified by immunofluorescence in 22 children admitted to the picu over a 16-month period. the medical records of these children were reviewed retrospectively, and their clinical and laboratory data were compare ... | 2011 | 21449904 |
| lung function prior to viral lower respiratory tract infections in prematurely born infants. | prematurely born infants who develop respiratory syncytial virus (rsv) lower respiratory tract infections (lrtis) have lung function abnormalities at follow-up. the aim of this study was to determine whether prematurely born infants who developed symptomatic rsv, or other viral lrti(s), had poorer premorbid lung function than infants who did not develop lrtis during the rsv season. | 2011 | 21447496 |
| part 4. interaction between air pollution and respiratory viruses: time-series study of daily mortality and hospital admissions in hong kong. | background: populations in asia are not only at risk of harm to their health through environmental degradation as a result of worsening pollution problems but also constantly threatened by recurring and emerging influenza epidemics and. pandemics. situated in the area with the world's fastest growing economy and close to hypothetical epicenters of influenza transmission, hong kong offers a special opportunity for testing environmental management and public health surveillance in the region. in t ... | 2010 | 21446214 |
| circulating human antibody-secreting cells during vaccinations and respiratory viral infections are characterized by high specificity and lack of bystander effect. | surges of serum abs after immunization and infection are highly specific for the offending ag, and recent studies demonstrate that vaccines induce transient increases in circulating ab-secreting cells (ascs). these ascs are highly enriched but not universally specific for the immunizing ag, suggesting that a fraction of these ascs could arise from polyclonal bystander stimulation of preexisting memory cells to unrelated ags. this model is proposed to explain maintenance of long-lived serological ... | 2011 | 21441455 |
| enhancement of respiratory mucosal anti-viral defenses by iodide oxidation. | recent reports postulate that the dual oxidase (duox) proteins function as part of a multi-component oxidative pathway used by the respiratory mucosa to kill bacteria. the other components include epithelial ion transporters, which mediate the secretion of the oxidizable anion thiocyanate (scn-) into airway surface liquid, and lactoperoxidase (lpo), which catalyzes the h2o2-dependent oxidation of the pseudohalide scn- to yield the antimicrobial molecule hypothiocyanite (oscn-). we hypothesized t ... | 2011 | 21441383 |
| intrinsic apoptotic pathway is subverted in mouse macrophages persistently infected by rsv. | to persist, a virus must co-exist with the host that it infects, thus allowing the virus to survive and to subvert the programmed cell death of the host. in this study, we investigated whether the intrinsic pathway of the apoptotic process is suppressed in a previously reported macrophage cell line persistently infected with respiratory syncytial virus (rsv). to this end, after using staurosporine to induce apoptosis, we determined cell viability and the degree of annexin staining and dna fragme ... | 2011 | 21440589 |
| [isolation precautions in a tertiary paediatric hospital]. | isolation is one of the measures to prevent the spread of nosocomial infections. however, in children's hospitals, given the type of patients and infections, and the architectural features of hospital wards, isolation precautions are sometimes difficult to implement and enforce. | 2011 | 21439920 |
| atypical bacterial pathogen infection in children with acute bronchiolitis in northeast thailand. | atypical bacterial pathogens--including mycoplasma pneumoniae, chlamydophila pneumoniae, and chlamydia trachomatis--are important infectious agents of the respiratory system. most current information pertains to adults and little is known about the role of these organisms in lower respiratory tract infections among young children with acute bronchiolitis. | 2011 | 21439510 |
| therapeutic combination of sildenafil and iloprost in a preterm neonate with pulmonary hypertension. | while new pharmacological approaches have been demonstrated to effectively manage ph in adults, few reports have addressed ph treatment in neonates and infants. this case report describes the successful management of severe ph secondary to bronchopulmonary dysplasia, respiratory syncytial virus infection, and hypoxia in a preterm 4-month-old with the long-term use of orally administered sildenafil and inhaled iloprost. | 2011 | 21438177 |
| respiratory syncytial virus prevention and therapy: past, present, and future. | respiratory syncytial virus (rsv) is the most common respiratory pathogen in infants and young children worldwide. more than 50 years after its discovery, and despite relentless attempts to identify pharmacological therapies to improve the clinical course and outcomes of this disease, the most effective therapy remains supportive care. although the quest for a safe and effective vaccine remains unsuccessful, pediatricians practicing during the past decade have been able to protect at least the m ... | 2011 | 21438168 |
| viral pneumonia. | about 200 million cases of viral community-acquired pneumonia occur every year-100 million in children and 100 million in adults. molecular diagnostic tests have greatly increased our understanding of the role of viruses in pneumonia, and findings indicate that the incidence of viral pneumonia has been underestimated. in children, respiratory syncytial virus, rhinovirus, human metapneumovirus, human bocavirus, and parainfluenza viruses are the agents identified most frequently in both developed ... | 2011 | 21435708 |
| cigarette smoke suppresses tlr-7 stimulation in response to virus infection in plasmacytoid dendritic cells. | exposure to environmental tobacco smoke (ets) is associated with an increase in the frequency and severity of respiratory infections, including bronchiolitis, a clinical syndrome of infancy caused by viruses such as respiratory syncytial virus (rsv). the mechanisms by which ets increases the risk of viral respiratory infections are largely unknown. a major effector integrating early antiviral and immunostimulatory activities is interferon-+¦ (ifn-+¦), which is highly produced by plasmacytoid den ... | 2011 | 21435390 |
| hot topics in the prevention of respiratory syncytial virus disease. | the 7th international respiratory syncytial virus symposium took place in hotel blijdorp, rotterdam, the netherlands. the series has been running since 1996; this meeting took place after a 3-year gap, and was attended by approximately 200 clinicians, scientists and industry representatives from all over the world. the conference covered all aspects of respiratory syncytial virus disease, including virology, cell biology, pathogenesis, clinical presentation, diagnosis, immunology, vaccines, anti ... | 2011 | 21434796 |
| molecular epidemiology of human respiratory syncytial virus in iran. | 2011 | 21434709 | |
| intranasal delivery of antiviral sirna. | intranasal administration of synthetic sirna is an effective modality of rnai delivery for the prevention and therapy of respiratory diseases, including pulmonary infections. vehicles used for nasal sirna delivery include established as well as novel reagents, many of which have been recently optimized. in general, they all promote significant uptake of sirna into the lower respiratory tract, including the lung. when properly designed and optimized, these sirnas offer significant protection agai ... | 2011 | 21431695 |
| acute-phase proteins: as diagnostic tool. | the varied reactions of the host to infection, inflammation, or trauma are collectively known as the acute-phase response and encompass a wide range of pathophysiological responses such as pyrexia, leukocytosis, hormone alterations, and muscle protein depletion combining to minimize tissue damage while enhancing the repair process. the mechanism for stimulation of hepatic production of acute-phase proteins is by proinflammatory cytokines. the functions of positive acute-phase proteins (app) are ... | 2011 | 21430962 |
| bimodal effects of obesity ratio on disease duration of respiratory syncytial virus infection in children. | background: morbid obesity may be associated with hospitalization and possibly death from the 2009 pandemic h1n1 infection, suggesting a yet unknown association between obesity and the severity of viral infections. thus, we examined association between obesity ratios and duration of disease in children with respiratory syncytial virus (rsv) infection. methods: a retrospective survey of 243 children admitted for bronchitis, bronchiolitis, pneumonia, and those who tested positive for a rsv test, w ... | 2011 | 21430434 |
| [information of the center for ecology and epidemiology of influenza, d. i. ivanovsky research institute of virology, russian academy of medical sciences, on the results of the 2009-2010 influenza and acute respiratory viral infection epidemic season (at week 40 of 2009 to week 22 of 2010) in the world and russia]. | the paper describes the specific features of the 2009-2010 epidemic season in russia and the world, which are due to the wide spread of a new pandemic strain of influenza a(h1n1)v virus. there is an unusual early upsurge in the incidence of influenza and acute respiratory viral infection (arvi) (in october-november 2009) with its peak at weeks 45 to 48 of the year with a succeeding reduction to the seasonal values by its end. the circulation of influenza b virus strains was recorded in february- ... | 2011 | 21427956 |
| [clinical features of 3 cases with acute interstitial pneumonia in children]. | acute interstitial pneumonia (aip) is a rare lung interstitial disease in children. this study was conducted to understand the clinical features of the aip in children. | 2011 | 21426685 |
| respiratory syncytial virus: a prioritized or neglected target? | 2010 | 21426146 | |
| nunavut government assailed over outbreak. | 2011 | 21422122 | |
| [effect of polyi: c on secretion of thymic stromal lymphopoietin and airway inflammation in mice with respiratory syncytial virus-induced asthma exacerbation]. | to investigate the effects of polyinosinic-polycytidylic acid (polyi:c) on the production of thymic stromal lymphopoietin (tslp) and airway inflammation in mice with exacerbated asthma induced by respiratory syncytial virus (rsv). | 2011 | 21421477 |
| adult outpatient experience of the 2009 h1n1 pandemic: clinical course, pathogens, and evaluation of case definitions. | the aim was to describe causative agents and clinical characteristics in adult outpatients with upper airway symptoms during the 2009 h1n1 pandemic and to evaluate case definitions that are used in clinical practice. | 2011 | 21420427 |
| inhaled ribavirin therapy in adult respiratory syncytial virus-induced acute respiratory distress syndrome. | respiratory syncytial virus (rsv) infection-induced acute respiratory distress syndrome (ards) in previously healthy adults is rare, but the overall mortality rate is 40-60%. inhaled ribavirin is approved for the treatment of hospitalized infants and young children with severe lower respiratory tract infections due to rsv. we present the case of an adult female with rsv pneumonia-induced ards who was successfully treated with inhaled ribavirin and whose pulmonary function was restored to near no ... | 2011 | 21420217 |
| respiratory syncytial virus load and disease severity in the community. | 2011 | 21412798 | |
| validation of statistical models for estimating hospitalization associated with influenza and other respiratory viruses. | reliable estimates of disease burden associated with respiratory viruses are keys to deployment of preventive strategies such as vaccination and resource allocation. such estimates are particularly needed in tropical and subtropical regions where some methods commonly used in temperate regions are not applicable. while a number of alternative approaches to assess the influenza associated disease burden have been recently reported, none of these models have been validated with virologically confi ... | 2011 | 21412433 |
| comparison of the luminex xtag respiratory viral panel with xtag respiratory viral panel fast for diagnosis of respiratory virus infections. | nucleic acid tests are sensitive and specific and provide a rapid diagnosis, making them invaluable for patient and outbreak management. multiplex pcr assays have additional advantages in providing an economical and comprehensive panel for many common respiratory viruses. previous reports have shown the utility of the xtag respiratory viral panel (rvp) assay manufactured by luminex molecular diagnostics for this purpose. a newer generation of this kit, released in canada in early 2010, is design ... | 2011 | 21411570 |
| real-time pcr-based detection of bordetella pertussis and bordetella parapertussis in an irish paediatric population. | novel real-time pcr assays targeting the bordetella pertussis insertion sequence is481, the toxin promoter region and bordetella parapertussis insertion sequence is1001 were designed. pcr assays were capable of detecting ôëñ10 copies of target dna per reaction, with an amplification efficiency of ôëñ90ôçè%. from september 2003 to december 2009, per-nasal swabs and nasopharyngeal aspirates submitted for b. pertussis culture from patients ôëñ1 month to >15 years of age were examined by real-time p ... | 2011 | 21393459 |
| molecular epidemiology and evolution of human respiratory syncytial virus and human metapneumovirus. | human respiratory syncytial virus (hrsv) and human metapneumovirus (hmpv) are ubiquitous respiratory pathogens of the pneumovirinae subfamily of the paramyxoviridae. two major surface antigens are expressed by both viruses; the highly conserved fusion (f) protein, and the extremely diverse attachment (g) glycoprotein. both viruses comprise two genetic groups, a and b. circulation frequencies of the two genetic groups fluctuate for both viruses, giving rise to frequently observed switching of the ... | 2011 | 21390255 |
| a respiratory syncytial virus replicon that is noncytotoxic and capable of long-term foreign gene expression. | respiratory syncytial virus (rsv) infection of most cultured cell lines causes cell-cell fusion and death. cell fusion is caused by the fusion (f) glycoprotein and is clearly cytopathic, but other aspects of rsv infection may also contribute to cytopathology. to investigate this possibility, we generated an rsv replicon that lacks all three of its glycoprotein genes and so cannot cause cell-cell fusion or virus spread. this replicon includes a green fluorescent protein gene and an antibiotic res ... | 2011 | 21389127 |
| respiratory syncytial virus- and influenza virus-associated hospitalizations in infants less than 12 months of age. | infants hospitalized because of respiratory syncytial virus (rsv) infection (n = 388) were significantly younger, had longer hospital stays, had a more severe course of disease, and required supplemental oxygen more often with longer duration of treatment as compared with those with influenza virus (n = 37) infection. seasonal distribution varied, with rsv-associated hospitalizations peaking in january and influenza virus-associated hospitalizations in february. congenital heart disease was more ... | 2011 | 21386747 |
| reduced dicer expression in the cord blood of infants admitted with severe respiratory syncytial virus disease. | respiratory syncytial virus (rsv) is one of the most important causes of pediatric hospital admissions in the developed world. the ribonuclease dicer is an important regulator of gene expression and cellular function via rna interference, and may also have anti-viral functions. a previous microarray analysis of the cord blood of 5 patients with rsv disease suggested downregulation of dicer. in order to further investigate whether reduced dicer expression can predispose newborns to rsv disease, w ... | 2011 | 21385408 |
| [a single-center prospective study of viral infection in community-acquired pneumonia in adults]. | from july, 2008 to march, 2009, 125 adults with community-acquired pneumonia (cap) who were admitted to our hospital were retrospectively investigated to elucidate the characteristics of viral infection in adult cap in japan. nasopharyngeal swabs for real-time polymerase chain reaction for 7 types of influenza virus, rhinovirus, respiratory synctial virus, human metapneumovirus, parainfluenza virus, coronavirus, and enterovirus were obtained. diagnoses of viral infections were established accord ... | 2011 | 21384676 |
| neutralization of hiv-1 infection of human peripheral blood mononuclear cells (pbmc) : antibody dilution method. | antibodies elicited by passive or active immunization protect against numerous virus diseases. this in vivo protective immunity is often associated with in vitro detection of neutralizing antibodies (nab) (e.g., polio, measles, influenza, respiratory syncytial virus, yellow fever, dengue, rabies, varicella, hepatitis a, hepatitis b) (1-7). since human protection from hiv-1-associated disease has not yet been achieved, the role of nab in protective immunity is not defined. nonetheless, based on e ... | 1999 | 21380682 |
| first principles modeling of nonlinear incidence rates in seasonal epidemics. | in this paper we used a general stochastic processes framework to derive from first principles the incidence rate function that characterizes epidemic models. we investigate a particular case, the liu-hethcote-van den driessche's (lhd) incidence rate function, which results from modeling the number of successful transmission encounters as a pure birth process. this derivation also takes into account heterogeneity in the population with regard to the per individual transmission probability. we ad ... | 2011 | 21379320 |
| pediatric respiratory diseases: 2011 update for the rogers' textbook of pediatric intensive care. | to review articles relevant to the field of pediatric respiratory disease that were published after the 2008 rogers' textbook of pediatric intensive care. | 2011 | 21378592 |
| activation of protein kinase r is required for induction of stress granules by respiratory syncytial virus but dispensable for viral replication. | we performed experiments to determine the effect of pkr activation on respiratory syncytial virus (rsv) replication. we first determined that rsv infection activates pkr which induces the phosphorylation of eif2a, resulting in the formation of host stress granules. we used rna interference to decrease endogenous pkr levels. rsv replication was not altered in cells deficient for pkr expression. however, rsv-mediated stress granule formation was significantly reduced in pkr-knockdown cells. as an ... | 2011 | 21377708 |
| the probability of extinction in a bovine respiratory syncytial virus epidemic model. | backward bifurcation is a relatively recent yet well-studied phenomenon associated with deterministic epidemic models. it allows for the presence of multiple subcritical endemic equilibria, and is generally found only in models possessing a reasonable degree of complexity. one particular aspect of backward bifurcation that appears to have been virtually overlooked in the literature is the potential influence its presence might have on the behaviour of any analogous stochastic model. indeed, the ... | 2011 | 21376734 |
| [severe bronchiolitis. changes in epidemiology and respiratory support]. | bronchiolitis generates lots of admissions during the cold months in the paediatric intensive care units (picu). new forms of respiratory support are being used and could improve the care of these patients. | 2011 | 21376684 |
| exhaled breath condensate sampling is not a new method for detection of respiratory viruses. | exhaled breath condensate (ebc) sampling has been considered an inventive and novel method for the isolation of respiratory viruses. | 2011 | 21375748 |
| use of lectins for characterization of o-linked glycans of herpes simplex virus glycoproteins. | the existence of o-linked glycans in viral glycoproteins was described in the early 1980s for enveloped viruses such as herpes simplex virus type 1 (hsv-1), vaccinia virus, and mouse hepatitis virus (1-4). glycoprotein c of hsv-1 (designated gc-1) was demonstrated to contain domains, in which numerous o-linked glycans were concentrated to pronase-resistant clusters (5-7), thereby resembling the organization of mucins (8). thus glycoprotein, containing nine sites for n-linked glycosylation in add ... | 1998 | 21374459 |
| liquid chromatographic resolution of 3-amino-1,4-benzodiazepin-2-one derivatives on various pirkle-type chiral stationary phases. | the two enantiomers of n-acyl amide and n-ureide derivatives of 3-amino-5-phenyl-1,4-benzodiazepin-2-ones, which have been known to show anti-respiratory syncytial virus (rsv) activity, were resolved on seven different pirkle-type chiral stationary phases (csps) with the use of 10% isopropyl alcohol in hexane as a mobile phase. among the seven pirkle-type csps, the one based on (s)-leucine derivative named as n-phe-l-leu was found to be most successful, the separation factors (a) and the resolut ... | 2011 | 21365690 |
| emerging antibody products and nicotiana manufacturing. | antibody based products are not widely available to address multiple global health challenges due to high costs, limited manufacturing capacity, and long manufacturing lead times. nicotiana-based manufacturing of antibody products may now begin to address these challenges as a result of revolutionary advances in transient expression and altered glycosylation pathways. this review provides examples of emerging antibody-based products (mucosal and systemic) that could be competitive and commercial ... | 2011 | 21358287 |
| serious early childhood wheezing after respiratory syncytial virus lower respiratory tract illness in preterm infants. | respiratory syncytial virus (rsv) lower respiratory tract infection (lri) in early life has been associated with sustained airway hyperreactivity during childhood; however, corresponding data in premature infants are sparse. | 2010 | 21353110 |
| evaluation of an alternative chest physiotherapy method in infants with respiratory syncytial virus bronchiolitis. | we proposed a new chest physiotherapy (cpt) secretion clearance method to treat respiratory syncytial virus bronchiolitis in infants. our new cpt method consists of 15 prolonged slow expirations, then 5 provoked cough maneuvers. | 2011 | 21352671 |
| impact of pollution, climate, and sociodemographic factors on spatiotemporal dynamics of seasonal respiratory viruses. | seasonal viruses present a major cause of morbidity and mortality in temperate climates. through major pandemics and smaller annual epidemics, viruses such as influenza, respiratory syncytial virus (rsv) and human rhinovirus (hrv) result in lost school and work days for most that are infected and more serious complications for the immunocompromised. the reasons for these viruses showing strict seasonality include but are not limited to the influence of cold weather and humidity on virus particle ... | 2011 | 21348956 |
| caress: the canadian registry of palivizumab. | palivizumab is indicated for respiratory syncytial virus (rsv) prophylaxis in high-risk children. however, relatively little is known about the current use, compliance, and outcomes associated with this medication. | 2011 | 21343842 |
| the burden of infections by parainfluenza virus in hospitalized children in spain. | we designed a prospective study to describe the clinical impact of the parainfluenza viruse (piv) types detected in hospitalized children with respiratory tract infections from september 2008 to august 2010 in spain. piv infections were a significant proportion of viral respiratory detections (11.8% of cases). piv types 3 and 4 were most commonly detected. there were clinical differences between piv and respiratory syncytial virus infections. | 2011 | 21343837 |
| afebrile seizures associated with respiratory syncytial virus infection: a situation-related seizure disorder in early infancy. | 2011 | 21342339 | |
| viral etiology among the elderly presenting acute respiratory infection during the influenza season. | acute respiratory tract infections are the most common illness in all individuals. rhinoviruses have been reported as the etiology of more than 50% of respiratory tract infections worldwide. the study prospectively evaluated 47 elderly individuals from a group of 384 randomly assigned for acute respiratory viral infections (cold or flu) and assessed the occurrence of human rhinovirus (hrv), influenza a and b, respiratory syncytial virus and metapneumovirus (hmpv) in botucatu, state of são paulo, ... | 2011 | 21340401 |
| detection of human bocavirus and human metapneumovirus by real-time pcr from patients with respiratory symptoms in southern brazil. | the introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hmpv) and human bocavirus (hbov), in respiratory tract specimens. we have studied the occurrence of hmpv and hbov in the porto alegre (pa) metropolitan area, one of the southernmost cities of brazil, evaluating children with suspected lower respiratory tract infection from may 2007-june 2008. a real-time polymerase chain reaction method was used for amplification and de ... | 2011 | 21340356 |
| viral infections after kidney transplantation. | chronic immunosuppression, required to maintain allograft function postorgan transplant, predisposes transplant patients to a variety of viral infections. these can occur at every stage of post-transplantation. some infections, however, such as cytomegalovirus (cmv), epstein barr virus (ebv), or bk virus (bkv), tend to occur within months after transplantation. cmv infections can be easily prevented by prophylaxis therapy whereas evb or bkv infections can be prevented by lowering (when possible) ... | 2011 | 21336246 |
| synthesis and in vitro anti-hsv-1 activity of a novel hsp90 inhibitor bj-b11. | in this study, a novel hsp90 inhibitor bj-b11, was synthesized and evaluated for in vitro antiviral activity against several viruses. possible anti-hsv-1 mechanisms were also investigated. bj-b11 displayed no antiviral activity against coxsackievirus b(3) (cvb(3)), human respiratory syncytial virus (rsv) and influenza virus (h1n1), but exhibited potent anti-hsv-1 and hsv-2 activity with ec(50) values of 0.42±0.18 µm and 0.60±0.21 µm, respectively. additionally, the inhibitory effects of bj-b11 a ... | 2011 | 21334891 |
| use of palivizumab and infection control measures to control an outbreak of respiratory syncytial virus in a neonatal intensive care unit confirmed by real-time polymerase chain reaction. | respiratory syncytial virus (rsv) is a potentially life-threatening infection in premature infants. we report an outbreak involving four infants in the neonatal intensive care unit (nicu) of our hospital that occurred in february 2010. rsv a infection was confirmed by real-time polymerase chain reaction. palivizumab was administered to all infants in the nicu. there were no additional symptomatic cases and repeat rsv surveillance confirmed that there was no further cross-transmission within the ... | 2011 | 21330007 |
| monitoring epidemic viral respiratory infections using one-step real-time triplex rt-pcr targeting influenza a and b viruses and respiratory syncytial virus. | rapid and specific diagnosis of influenza a/b and respiratory syncytial virus (rsv) viruses is needed for optimal management of patients with acute respiratory infections. in this study, a one-step triplex real-time rt-pcr assay was developed for rapid diagnosis of influenza a/b and rsv infections to optimize diagnosis efficiency of acute respiratory infections. cell-culture supernatants and clinical samples were used to evaluate specificity and sensitivity of the assay. the assay was used routi ... | 2011 | 21328385 |
| trends in chronologic age and infant respiratory syncytial virus hospitalization: an 8-year cohort study. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infections in infants and young children and the leading cause of hospitalization in infants aged <1 year. | 2011 | 21327753 |
| early induction of autophagy in human fibroblasts after infection with human cytomegalovirus or herpes simplex virus 1. | the infection of human fetal foreskin fibroblasts (hfff2) with human cytomegalovirus (hcmv) resulted in the induction of autophagy. this was demonstrated by the increased lipidation of microtubule-associated protein 1 light chain 3 (lc3), a hallmark of autophagy, and by the visualization of characteristic vesicles within infected cells. the response was detected first at 2 h postinfection and persisted for at least 3 days. de novo protein synthesis was not required for the effect, since hcmv tha ... | 2011 | 21325419 |
| a novel capillary electrophoresis-based multiplex pcr assay for detection of respiratory pathogens. | the field of infectious disease testing has recently experienced rapid expansion in the number of multiplexed pcr-based assays available for detecting respiratory pathogens. this study provides a preliminary evaluation of a multiplex assay from seegene that uses capillary electrophoresis as the detection platform for viral and bacterial respiratory pathogens. we compared this technology to a real-time pcr assay for 3 viral targets. thirty respiratory samples were collected that had previously te ... | 2011 | 21325252 |
| rna interference, rsv, and lung transplantation: a promising future for sirna therapeutics. | 2011 | 21325081 | |
| prevention of serious respiratory syncytial virus-related illness. i: disease pathogenesis and early attempts at prevention. | respiratory syncytial virus (rsv) was first described 160 years ago but was not officially recognized as a cause of serious illness in children until the late 1950s. it has been estimated that virtually all children have had at least one rsv infection by their second birthday. rsv is responsible for annual disease outbreaks, usually during a defined winter seasonal period that can vary by community and year. rsv is recognized as the leading cause of hospitalization among young children worldwide ... | 2011 | 21318606 |
| prevention of serious respiratory syncytial virus-related illness. ii: immunoprophylaxis. | respiratory syncytial virus (rsv) causes significant morbidity in very young children, preterm infants with and without chronic lung disease, and children with hemodynamically significant congenital heart disease. in the absence of a safe and effective vaccine, alternative means of protecting high-risk infants and young children from serious rsv illness have been studied. clinical observations and animal model data over the past 30 years suggested that rsv immunoglobulin g (igg) neutralizing ant ... | 2011 | 21318605 |
| respiratory syncytial virus testing during bronchiolitis episodes of care in an integrated health care delivery system: a retrospective cohort study. | bronchiolitis has the highest incidence rate of any lower respiratory infection among infants and children <2 years of age. respiratory syncytial virus (rsv) is the most common etiology of bronchiolitis. the american academy of pediatrics does not recommend routine rsv testing for infants and children with bronchiolitis. the clinical predictors of rsv testing are unknown. objectives: the aims of this study were to identify the rates and predictors of rsv testing during bronchiolitis and to explo ... | 2010 | 21316538 |
| local innate and adaptive immune responses regulate inflammatory cell influx into the lungs after vaccination with formalin inactivated rsv. | inactivated respiratory syncytial virus (rsv) vaccines tend to predispose for immune mediated enhanced disease, characterized by th2 responses and airway hypersensitivity reactions. we show in a c57bl/6 mouse model that the early innate response elicited by the challenge virus (rsv versus influenza virus) influences the outcome of the th1/th2 balance in the lung after intramuscular priming with inactivated vaccine. priming of cd4(+)/ifn-?(+) t cells by mature dendritic cells administered intrave ... | 2011 | 21316502 |
| health care-acquired viral respiratory diseases. | health care-associated viral respiratory infections, common among hospitalized children, also occur among adults and institutionalized persons and result in increased patient morbidity, mortality, and health care costs. approximately 20% of patients with healthcare-associated pneumonia have viral respiratory infections, with 70% of these infections caused by adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus (rsv). these infections typically reflect the level of vi ... | 2011 | 21316002 |
| [paroxysmal supraventricular tachycardia and severe respiratory syncytial virus infection in the neonatal period]. | 2011 | 21315670 | |
| large-scale seroprevalence analysis of human metapneumovirus and human respiratory syncytial virus infections in beijing, china. | human metapneumovirus (hmpv), a recently identified virus, causes acute respiratory tract infections (artis) in infants and children. however, studies on the seroepidemeology of hmpv are very limited in china. to assess the seroprevalence of hmpv infection in china, we tested a total of 1,156 serum specimens for the presence of anti-hmpv igg antibody in children and adults free of acute respiratory illness in beijing, china by using hmpv nucleocapsid (n) protein as an antigen. as a control, we u ... | 2011 | 21310026 |
| exogenous administration of vascular endothelial growth factor prior to human respiratory syncytial virus a2 infection reduces pulmonary pathology in neonatal lambs and alters epithelial innate immune responses. | human respiratory syncytial virus (rsv) affects thousands of children every year. vascular endothelial growth factor (vegf) is a regulator of vasculogenesis, pulmonary maturation, and immunity. in order to test the extent to which vegf may alter rsv infection, 4 groups of lambs received either human recombinant vegf (rhvegf) or phosphate-buffered saline (pbs) pretreatment followed by inoculation with human rsv strain a2 or sterile medium. lambs in each group were sacrificed at 2, 4, and 6 days p ... | 2011 | 21309731 |
| soluble respiratory syncytial virus fusion protein in the fully cleaved, pretriggered state is triggered by exposure to low-molarity buffer. | the paramyxovirus fusion (f) glycoprotein is anchored in the virion membrane in a metastable, pretriggered form. once triggered, the f protein undergoes a dramatic conformational extension that inserts its hydrophobic fusion peptide into the target cell membrane, then folds back on itself to bring the membranes together and initiate fusion. unlike most other paramyxoviruses, the respiratory syncytial virus (rsv) f protein alone is sufficient to mediate membrane fusion and virus infection. to stu ... | 2011 | 21307202 |
| both nonstructural proteins ns1 and ns2 of pneumonia virus of mice are inhibitors of the interferon type i and type iii responses in vivo. | infection of mice with pneumonia virus of mice (pvm) provides a convenient experimental pathogenesis model in a natural host for a human respiratory syncytial virus-related virus. extending our previous work showing that the pvm nonstructural (ns) proteins were pathogenicity factors in mice, we identify both the ns1 and ns2 proteins as antagonists of alpha/beta interferon (ifn-a/ß) and ifn-? by use of recombinant pvm (rpvm) with single and combined deletions of the ns proteins (?ns1, ?ns2, and ? ... | 2011 | 21307191 |
| influenza and other respiratory viruses in three central american countries. | despite the disease burden imposed by respiratory diseases on children in central america, there is a paucity of data describing the etiologic agents of the disease. | 2010 | 21306576 |
| using the full spectral capacity (six channels) of a real-time pcr instrument can simplify diagnostic laboratory screening and typing protocols for pandemic h1n1 influenza. | timely reporting of influenza a virus subtype affects patient management. real-time pcr is a rapid and sensitive method routinely used to characterise viral nucleic acid, but the full spectral capability of the instruments is not employed. | 2010 | 21306574 |
| mortality attributable to pandemic influenza a (h1n1) 2009 in san luis potosí, mexico. | acute respiratory infections are a leading cause of morbidity and mortality worldwide. starting in 2009, pandemic influenza a(h1n1) 2009 virus has become one of the leading respiratory pathogens worldwide. however, the overall impact of this virus as a cause of mortality has not been clearly defined. | 2010 | 21306570 |
| cost-effectiveness of respiratory syncytial virus prophylaxis in various indications. | to evaluate the cost-effectiveness of immunoprophylaxis against respiratory syncytial virus (rsv) infections with palivizumab based on actual cost and observed incidence rates in various pediatric risk groups. | 2011 | 21300647 |
| twelve years' detection of respiratory viruses by immunofluorescence in hospitalised children: impact of the introduction of a new respiratory picornavirus assay. | direct immunofluorescence assays (dfa) are a rapid and inexpensive method for the detection of respiratory viruses and may therefore be used for surveillance. few epidemiological studies have been published based solely on dfa and none included respiratory picornaviruses and human metapneumovirus (hmpv). we wished to evaluate the use of dfa for epidemiological studies with a long-term observation of respiratory viruses that includes both respiratory picornaviruses and hmpv. | 2011 | 21299840 |
| respiratory infections by hmpv and rsv are clinically indistinguishable but induce different host response in aged individuals. | human metapneumovirus and respiratory syncytial virus can cause severe respiratory diseases, especially in infants, young children, and the elderly. so far it remains unclear why infections in the elderly become life threatening despite the presence of neutralizing antibodies in the serum, and to which extent double infections worsen the clinical course. | 2011 | 21298115 |
| primary human mdc1, mdc2, and pdc dendritic cells are differentially infected and activated by respiratory syncytial virus. | respiratory syncytial virus (rsv) causes recurrent infections throughout life. vaccine development may depend upon understanding the molecular basis for induction of ineffective immunity. because dendritic cells (dcs) are critically involved in early responses to infection, their interaction with rsv may determine the immunological outcome of rsv infection. therefore, we investigated the ability of rsv to infect and activate primary mdcs and pdcs using recombinant rsv expressing green fluorescen ... | 2011 | 21297989 |
| the relationship between rsv bronchiolitis and recurrent wheeze: the chicken and the egg. | respiratory syncytial virus bronchiolitis is the most frequent cause of infant hospitalization. rsv bronchiolitis is often followed by recurrent episodes of wheeze. pathogenesis of rsv bronchiolitis as well as post-bronchiolitis wheeze are incompletely understood. the aim of this review is to provide a brief overview of our current understanding of the complex pathogenesis of rsv bronchiolitis and post-bronchiolitis wheeze. two non-exclusive hypotheses exist, which are paraphrased for this revie ... | 2011 | 21295416 |
| viruses and asthma. | background: viral respiratory infection has long been known to influence the occurrence of asthma exacerbations. over the last 20years much effort has been put into clarifying the role that viral respiratory infections play in the eventual development of asthma. scope of review: in this review we give a general background of the role of viruses in the processes of asthma exacerbation and asthma induction. we review recent additions to the literature in the last 3years with particular focus on cl ... | 2011 | 21291960 |
| pandemic influenza a(h1n1) 2009: molecular characterisation and duration of viral shedding in intensive care patients in bordeaux, south-west france, may 2009 to january 2010. | from may 2009 to january 2010, the virology laboratory at the university hospital of bordeaux received more than 4,000 nasopharyngeal samples from the aquitaine region (south-west france) for the diagnosis of pandemic influenza a(h1n1)2009. eighty-three infected patients deteriorated and were admitted to intensive care units. our study focused on 24 of these patients. positivity for influenza a(h1n1)2009 was monitored by realtime pcr and duration of viral shedding was determined. the first avail ... | 2011 | 21284922 |