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the continent sigmoid urinary reservoir--an experimental study in pigs.to evaluate the function of a continence mechanism in combination with a colonic urinary reservoir in a pig model.19968944507
a karyotypic analysis of the lesser malay chevrotain, tragulus javanicus (artiodactyla: tragulidae).chevrotains are small forest-dwelling ruminants of the family tragulidae. the chromosome number of the lesser malay chevrotain was determined to be 2n = 32, nf = 64, g- and q-banding allowed the identification of homologous chromosomes, and c-banding demonstrated the presence of pericentromeric, telomeric and interstitial constitutive heterochromatin. q-band comparisons with domestic cattle revealed relatively few monobrachial chromosome band homologies. however, the smallest biarmed autosome of ...19968939367
husbandry and diseases of captive wild swine and peccaries.wild swine and peccaries are popular exhibit animals throughout the world, but the anatomy and behaviour of these species make it difficult to construct housing and enclosures which are suitable from an aesthetic and sanitation standpoint. african wild suids may serve as reservoir hosts for african swine fever. likewise, african suids are susceptible to most of the viral, bacterial and parasitic diseases of domestic swine. wild swine and peccaries are not an important source of zoonotic diseases ...19968924700
neonatal hemodynamic responses to extreme ranges of controlled graded hypoxia.to determine the hemodynamic responses to a wide range of specific, controlled, graded levels of hypoxic hypoxia over 120 mins in a neonatal porcine model and to identify the pao2 threshold for altered hemodynamic homeostasis.19968917041
repair of a mid-diaphyseal femoral fracture in a boar.a simple, oblique fracture of the middle portion of the diaphysis of the right femur was successfully repaired in a 10-month-old 150-kg berkshire boar, using a 4.5-mm 9-hole broad dynamic compression plate applied to the lateral surface of the bone. a lateral surgical approach provided excellent access to the fragment ends. intravenous administration of atracurium besylate facilitated traction along the long axis of the bone and fracture reduction. the plate was luted with 5% gentamicin sulfate- ...19968899028
location of external anal sphincter motoneurons in the sacral cord of the female domestic pig.the location of the striated external anal sphincter motoneurons in the spinal cord was investigated in 12, between 3 and 4 months old, female domestic pigs using the retrograde tracer horseradish peroxidase (hrp). their motoneuronal cell bodies were found in the spinal segments s1-s3, and were not located in the ventral horn, but dorsolateral to the central canal. this location within the spinal gray matter strongly differs from the location of the external and sphincter motoneurons in rat, cat ...19968897493
isolated coronary artery transplantation in pigs: a new model to study transplantation arteriosclerosis and humoral rejection.graft atherosclerosis is a major cause of death after heart transplantation; its causes are multifactorial and poorly understood. to determine whether specific sensitization to coronary artery antigens is a contributing factor, we developed an isolated coronary artery allotransplantation model in pigs.19968889988
effectiveness of fibrin glue in the reduction of postoperative intrapericardial adhesions.the hemostatic properties of fibrin sealant have been well described. previously published reports have attempted to clarify the possible role of fibrin glue in the inhibition of the formation of intrapericardial adhesions following cardiac surgery. earlier work hypothesized that fibrin glue may reduce the severity of postoperative adhesions and that the use of autologous fibrin glue may have similar effects, without the risks that accompany homologous blood products. six juvenile farm pigs were ...19968887071
transorbital approach to the porcine pituitary.a transorbital approach to the pituitary gland is described in domestic swine weighing between 40 and 70 kg. a transpalpebral eye exenteration is performed and the optic canal is enlarged caudally, using a bone drill. an operating microscope is used to improve visualization of the surgical site as the pituitary stalk and anterior pituitary are exposed to the level of the optic chiasm. this approach exposes the pituitary sufficiently to perform either a hypophyseal stalk transection or a hypophys ...19968887068
the prepubertal ontogeny of luteinizing hormone releasing hormone-like immunoreactivity in the diencephalon and telencephalon of the male meishan pig brain.luteinizing hormone releasing hormone (lhrh) is a decapeptide that regulates reproductive function and behaviors in mammalian species. because of the importance of proper reproductive function and efficiency in agricultural species, we have investigated the prepubertal ontogeny of lhrh-like immunoreactivity (ir) in the male meishan pig. the meishan is a chinese breed known for reproductive traits including increased litter size and precocious puberty, but slow growth and obesity. brains of anima ...19968874890
starling resistor effects on pulmonary artery occlusion pressure in endotoxin shock provide inaccuracies in left ventricular compliance assessments.assessment of left ventricular preload and left ventricular compliance changes in septic shock using pulmonary artery occlusion pressure (paop) presumes that this pressure accurately reflects left heart filling pressure. we tested the hypothesis that starling resistor forces render paop inaccurate as an index of left heart filling pressure, resulting in misleading assessments of left ventricular compliance changes.19968874296
the soft tissue cover of the mandibular condyle: age-related changes in high buoyant density proteoglycans, free tissue water and remodelling activity.age-related changes in the relative proportion of high buoyant density proteoglycans of the soft tissue cover of the mandibular condyle of domestic pigs and the chemical composition of these proteoglycans were investigated by biochemical methods. the relative proportion of high buoyant density proteoglycans has been shown to decrease with age, with their chemical composition varying as follows: with age, the relative proportions of protein increased, galactosamine decreased, glucosamine was simi ...19968874176
a comparison of the pathophysiologic effects of carbon dioxide, nitrous oxide, and helium pneumoperitoneum on intracranial pressure.previous studies have suggested that diagnostic laparoscopy may be contraindicated in multiple trauma patients with closed head injuries because of the detrimental effects of carbon dioxide (co2) pneumoperitoneum on intracranial pressure (icp). in this study we compared the effects of two alternative inflation gases, helium (he) and nitrous oxide (n2o), against the standard agent used in most hospitals, co2. icp was monitored in experimental animals both with and without a space occupying intrac ...19968862077
protection of non-murine mammals against encephalomyocarditis virus using a genetically engineered mengo virus.genetically engineered mengo viruses with artificial deletions in the 5' noncoding poly(c) tracts are highly attenuated for pathogenicity when introduced as live vaccines into the natural murine host. inoculation produces lifelong protective immunity without disease or viral persistence. this report extends the vaccination studies to non-murine hosts, including baboons, macaques and domestic pigs, all of which are susceptible to severe cardiovirus epizootics. all animals of these species that we ...19968852413
characterisation of bradykinin receptors from juvenile pig coronary artery.coronary artery rings from juvenile male farm pigs were incubated for 6 h and precontracted with u46619. the rings relaxed in response to des-arg9-bradykinin (pd2, 7.78 +/- 0.13; emax, 87.4 +/- 4.3%) and to bradykinin (pd2, 8.69 +/- 0.30; emax, 104.2 +/- 4.4%). these responses were abolished by endothelium removal and unaffected by indomethacin whilst ng-nitro-l-arginine reduced the relaxation due to des-arg9-bradykinin only. preincubation with cycloheximide or actinomycin had no effect against ...19968851166
serum concentrations of melatonin in prepubertal gilts exposed to either constant or stepwise biweekly alteration in scotophase.melatonin (mel), a hormone known to mediate photoperiodic cues, is secreted from the pineal gland in a circadian fashion in numerous species. the transduction of photoperiodic information into the secretion of mel, however, remains controversial in the pig. to determine whether domestic pigs have a nocturnal increase in serum melatonin when exposed to equatorial photoperiods only, 24 prepubertal gilts (38.7 +/- 0.7 kg; 104.5 +/- 0.8 d) and 12 mature ewes, serving as positive controls, were rando ...19968839625
an outbreak of japanese encephalitis in the torres strait, australia, 1995.to determine the distribution of virus infection during an outbreak of japanese encephalitis (je) in the torres strait, and to describe the environmental factors facilitating the outbreak.19968816682
geographic population structure and sequence divergence in the mitochondrial dna control region of the japanese wild boar (sus scrofa leucomystax), with reference to those of domestic pigs.mitochondrial dna (mtdna) control regions from 40 japanese wild boars were examined by direct sequencing after amplification by pcr. from the dna sequences obtained, we found eight haplotypes, whose differences arose via transitions. the geographical distribution of these different haplotypes indicated that wild boar populations inhabited limited areas and that there was some restricted gene flow between local populations. eight mtdna haplotypes from eastern and western domestic pigs and the ryu ...19968813051
the immunogenic and pathogenic potential of short poly(c) tract mengo viruses.we have shown previously that genetically engineered mengo viruses with artificial deletions in their 5' noncoding polyribocytidylic acid (poly(c)) tracts are highly attenuated for the natural murine host and also for other animals such as baboons, macaques, and domestic pigs. the present report further characterizes select short poly(c) tract mengo viruses in the natural murine host. a positive correlation was found between the length of the poly(c) tract and murine virulence, as measured by vi ...19968806569
heritability estimate of hypertrophic cardiomyopathy in pigs (sus scrofa domestica).the purpose of this study was to evaluate the heritability of hypertrophic cardiomyopathy (hcm) in pigs and the relation between hcm and heart measurements, pathologic features, and growth to provide references for hcm line development. a total of 353 on-farm tested gilts (females) and boars (males) from 74 sire families were randomly selected from a single breeding farm where hcm was prevalent. hearts were collected after animals were slaughtered. heart length, width, and weight, heart-to-body ...19968799938
increased concentrations of endothelin-1 messenger rna in tissues and endothelin-1 peptide in plasma in septic pigs: modulation by betamethasone.to study the expression of preproendothelin-1 messenger rna (mrna) in tissue after escherichia coli lipopolysaccharide challenge and to evaluate the possible effects of betamethasone both regarding endothelin-1 production as well as hemodynamic and vascular effects during e. coli lipopolysaccharide infusion in pigs in vivo.19968797627
effects of intracoronary carbon dioxide on left ventricular function in swine.carbon dioxide is currently used as an arterial and venous contrast agent; however, little is known of its effects on left ventricular function. this study was undertaken to investigate those effects.19968790949
immunocytochemical distribution of catecholamine-synthesizing neurons in the hypothalamus and pituitary gland of pigs: tyrosine hydroxylase and dopamine-beta-hydroxylase.this study describes the distribution of catecholaminergic neurons in the hypothalamus and the pituitary gland of the domestic pig, sus scrofa, an animal that is widely used as an experimental model of human physiology in addition to its worldwide agricultural importance. hypothalamic catecholamine neurons were identified by immunocytochemical staining for the presence of the catecholamine synthesizing enzymes, tyrosine hydroxylase and dopamine-beta-hydroxylase. tyrosine hydroxylase-immunoreacti ...19968789282
sus scrofa domestica endoparasitic resistance in the amazonas.the domestic pig (sus scrofa domestica l.) has been wild breeding in the varzea amazonian estuary area. without dietary supplement or medical prevention, the pigs survive, bringing economic benefits to local peasants. endoparasitic diseases generate economic losses because of neonatal death and clinical disease. we assessed the nematoda (o. nematoda) and coccidia (o. eucoccidia) parasitic level and tolerance in 75 pigs (0-18 months) from 35 families using the following methods: (i) larvae cultur ...19968784532
alternative methods of exposure minimize cardiopulmonary risk in experimental animals during minimally invasive surgery.alternative methods of exposure are needed for minimally invasive surgery to avoid hypercarbia and acidosis associated with carbon dioxide (co2) abdominal insufflation. the goals of this study were to determine the pulmonary and hemodynamic effects of both helium (he) abdominal insufflation and placement of a mechanical abdominal wall-lifting device (lifter) during laparoscopy.19968779063
effects of hypercholesterolaemia on physiological recruitment of coronary vascular reserve in swine.1. the endothelium participates in the regulation of coronary vascular tone. as evidence exists from studies performed on epicardial vessels that hypercholesterolaemia impairs endothelial function, we tested the hypothesis that hypercholesterolaemia impairs coronary vascular reserve in an intact animal. 2. domestic swine, maintained on a regular (n = 9) or a 2% high-cholesterol (n = 9) diet for 3 months were instrumented with a catheter in the left atrium for microsphere injection, a catheter in ...19968777832
electromyographic and mechanomyographic characteristics of neuromuscular block by magnesium sulphate in the pig.in spite of its well known propensity to cause accidental paralysis by a specific mechanism of action, mg(2+)-induced neuromuscular block has not been examined systematically for its characteristics of muscle response to nerve stimulation. we examined in seven anaesthetized domestic pigs the mechanomyographic (mmg) and neurally evoked compound electromyographic (ncemg, emg) responses of the tibialis anterior muscle to stimulation of its motor nerve, at baseline and during three levels of neuromu ...19968777111
a porcine model of chronic peripheral arterial occlusion.to create a simple and reproducible model of chromic thrombosis for the evaluation of thrombolytic agents and devices.19968773977
myocardial adaptation during and after sustained, demand-induced ischemia. observations in closed-chest, domestic swine.we tested the hypotheses that prolonged, demand-induced myocardial ischemia plateaus and that on relief of stress, myocardial function remains depressed, with proportionate reductions in blood flow and oxygen consumption indicative of hibernation.19968772699
age dependence of cerebrovascular response mechanisms in domestic pigs.hypercapnia-induced cerebral vasodilation in the newborn pig is a prostanoid-associated response. in some adult models, hypercapnic cerebral vasodilation is associated with the generation of nitric oxide (no). acetylcholine (ach) produces a no-dependent cerebral vasodilation in many adult models, but topical ach is a prostanoid-associated cerebral vasoconstrictor in the newborn pig. we hypothesized that mediators influencing cerebral response can be age dependent. juvenile domestic pigs were com ...19968770094
an epidemiological study of influenza viruses among chinese farm families with household ducks and pigs.to examine the possibility of interspecies transmission and genetic reassortment of influenza viruses on farms in southern china, we surveyed 20 farm families living outside the city of nanchang who raised pigs and ducks in their homes. weekly interviews of family members and virus isolation studies of throat swabs and faecal samples, collected from september 1992 to september 1993, established the seasonal pattern of respiratory tract infections in these families and identified 11 influenza vir ...19968760967
myocardial dysfunction after resuscitation from cardiac arrest: an example of global myocardial stunning.this study investigated the effect of prolonged cardiac arrest and subsequent cardiopulmonary resuscitation on left ventricular systolic and diastolic function.19968752819
four alginate dressings in the treatment of partial thickness wounds: a comparative experimental study.alginate-based absorption materials for wound treatment are meeting with increasing clinical acceptance. the purpose of this study was to compare four different calcium alginate dressings (algosteril, comfeel alginate, kaltostat and sorbsan) with respect to wound fluid retaining ability, adherence, dressing residues, epithelialisation and inflammatory cell infiltration using a standardised partial-thickness wound model in domestic pigs. wound fluid spread laterally onto surrounding normal skin b ...19968733355
effects of graded doses of vasopressin on median fibrillation frequency in a porcine model of cardiopulmonary resuscitation: results of a prospective, randomized, controlled trial.to assess the effects of graded doses of vasopressin vs. saline on median fibrillation frequency and defibrillation success in a porcine model of cardiopulmonary resuscitation.19968706492
endotoxic shock alters distribution of blood flow within the intestinal wall.to investigate whether a redistribution of blood flow from the mucosa to the muscular layer of the intestinal wall contributes to the observed increased arterial-mucosal pco2 gradient and the decreased mucosal tonometric ph during endotoxic shock.19968706490
evaluation of the effects of autotransfusion of unprocessed blood on hemodynamics and oxygen transport in anesthetized pigs.to examine the hemodynamic effects and the oxygen transport pattern of autotransfusion of unprocessed blood on hemodynamics and oxygen transportation.19968706465
[observational and experimental studies on relationship between domestic pigs and epidemic hemorrhagic fever investigation].observational and experimental studies on the relationship between domestic pigs and epidemic hemorrhagic fever (ehf) were carried out during 1986 to 1994 with reverse passive hemagglutination (rpha), reverse passive hemagglutination inhibition (rphi), horse-radish peroxidase-staphylococcal protein a (hrp-spa), indirect immunofluorescence assay (ifa) and reverse transcript polymerase chain reaction (rt-pcr) to understand the roles of domestic pigs played in the epidemic areas. results showed dom ...19958697938
recent fusion events during evolution of pig chromosomes 3 and 6 identified by comparison with the babirusa karyotype.the chromosomes of the babirusa, a species considered to have diverged from an ancestor of the pig during the miocene epoch, about 12-26 million years ago, were studied to determine the sites of recent rearrangements during evolution of the domestic pig. it is shown that there is a pericentric inversion of the entire short arm on pig chromosome 1, compared to its counterpart in the babirusa (chromosome 15). we also present evidence suggesting that pig chromosome 3 was derived by a telomere-centr ...19968697808
experimental application of controlled limb reperfusion after incomplete ischaemia.severe local and systemic complications may occur after revascularization of extremities exposed to prolonged complete or incomplete ischaemia. these complications may be reduced by controlling the reperfusate and the circumstances of the reperfusion period. ten adult german domestic pigs were exposed to 6 h of incomplete limb ischaemia by occlusion of the left iliac artery. to simulate the clinical situation of embolectomy, the occlusive snares were released after the ischaemic period in five p ...19968696746
myocardial dysfunction after successful resuscitation from cardiac arrest.to investigate the functional and metabolic changes in the myocardium after successful resuscitation from cardiac arrest.19968681604
splanchnic and peripheral tissue perfusion in experimental fat embolism.to investigate the acute effects of experimental fat embolism on splanchnic and peripheral perfusion and oxygenation in pigs.19968681568
radiolabeled iododeoxyuridine: safety evaluation.the emphasis of radiolabeled iododeoxyuridine (*iudr) research at our institution to date has been to assess its safety as a potential therapeutic agent. toward this goal, we have performed preclinical and clinical studies, using various routes of administration, to detect adverse changes in normal tissues in both humans and animals. as iudr is rapidly dehalogenated by the liver, the intravenous route is unlikely to be successful in therapeutic efforts. we have therefore focused our attention on ...19968676196
controlled trial of laparoscopic-assisted vs open colon resection in a porcine model.several series of laparoscopic colon resection have been reported in the literature with varied results; however, no controlled series of laparoscopic vs open colon resection has been reported. the purpose of this study was to determine the relative safety and adequacy of laparoscopic colon resection in a controlled trial using a porcine model.19968662429
comparison of self-expanding polyethylene terephthalate and metallic stents implanted in porcine iliac arteries.comparison of the biocompatibility of self-expanding polyethylene terephthalate (pet) stents with self-expanding metallic stents (wallstents).19968661640
[zoo-anthroponoses in vojvodina. iv. epidemiologic characteristics of trichinosis in vojvodina].epidemiological characteristics of trichinellosis in vojvodina have been analyzed on the basis of registered cases of the disease, registration of epidemics and also epidemiological investigation of exposed persons. in the period 1984-1993, 1802 diseased persons were registered, 958 male and 844 female. death was registered in one case. average morbidity was 8.9/10(5). trichinellosis affects all age groups. at younger age the disease affects both sexes, but in the group over 20 years of age the ...19958657070
iced temperature injectate for thermodilution cardiac output determination causes minimal effects on cardiodynamics.controversy exists regarding the ideal injectate temperature for measuring cardiac output. iced temperature injectate gives a higher signal/noise ratio and less variability in the measured cardiac output. thus, less volume and fewer measurements are required. advocates of room temperature injectate have suggested that iced temperature injectate may perturb cardiodynamics. this concern has remained largely untested. to help resolve this controversy, we examined the effects of 5 ml iced injectate ...19968625640
hyperglycemia results in an increase in myocardial interstitial glucose and glucose uptake during ischemia.the purpose of this investigation was to assess the effects of hyperglycemia, in the absence of changes in plasma insulin and arterial free fatty acid (ffa) levels, on interstitial glucose levels and glucose uptake across the left ventricular wall during ischemia in domestic swine. insulin secretion was suppressed with a continuous infusion of somatostatin. arterial ffa levels remained stable due to the suppression of insulin. microdialysis probes were used to estimate changes in interstitial gl ...19968622595
structure and organization of pig mhc class ii drb genes: evidence for genetic exchange between loci.the pig major histocompatibility complex drb genes were studied by polymerase chain reaction (pcr) amplification of exon 2 from eight domestic pigs and two european wild boars. sequence comparisons together with a phylogenetic analysis showed the existence of at least three drb genes of which only one appears to be expressed. the two putative drb pseudogenes contained deletions in exon 2, making it possible to confirm the presence of three non-allelic drb genes by analyzing the length polymorphi ...19968613138
myocardial cell death in reperfused and nonreperfused myocardial infarctions. mr imaging with dysprosioum-dtpa-bma in the pig.to investigate whether dy-dtpa-bma-enhanced mr imagining would permit identification of myocardial cell death, myocardial infarction was induced in 12 domestic pigs.19968611317
interferon, the oxytocin receptor and the maternal recognition of pregnancy in ruminants and non-ruminants: a comparative approach.ruminant and non-ruminant members of the artiodactyla appear to use different mechanisms for the maternal recognition of pregnancy. in ruminants, the trophoblast of the developing blastocyst produces a characteristic type i interferon of the interferon-tau family; this acts by blocking endometrial synthesis of the oxytocin receptor. a similar mechanism applies to domestic ruminants (sheep, cow, goat) and deer. in roe deer, which have embryonic diapause and are monoestrus, there is no requirement ...19958606939
immunocytochemical studies on endocrine cells of alimentary tract of the pig in the embryonic and fetal period of life.the study aimed at immunocytochemical analysis of alimentary tract endocrine cells between 20th day of embryonal life and 105th day of fetal life of domestic pig. in the pancreas, presence of endocrine cells was detected already in 20th day and, at the time, the cells comprised around 3/4 all cells in primordia of the organ. starting at that time, numerous endocrine cells produced insulin and glucagon and individual cells synthesized somatostatin and pancreatic polypeptide. in the 20th day, stom ...19958606027
the effect of prolonged pneumoperitoneum on renal function in an animal model.prolonged, increased intra-abdominal pressure (iap) during laparoscopic surgery has been associated with oliguria and anuria.19968605555
spontaneous agonal respiration in a swine model of out-of-hospital cardiac arrest.to describe ventilatory dynamics following the onset of ventricular fibrillation (vf) in an experimental swine model.19958597915
serological study of pigs for antibody against african swine fever virus in two areas of southern malawi.a serological survey was conducted in july 1991 on domestic pigs in two areas of southern malawi which were severely affected by the african swine fever (asf) epizootic in 1989-1991. sixty-six of the 216 owners questioned reported having witnessed asf in their pigs. forty-seven owners had pigs with antibodies against asf virus, and the overall prevalence of pigs with anti-asf virus antibodies was found to be 12.4%, in 445 pigs sampled in 35 villages. spread of asf was thought to occur principall ...19958593400
[basic values of blood coagulation parameters in pigs (sus scrofa domesticus)].on 23 clinical healthy pigs (2-4 months of age, body weight 13-42 kg) under ketamin-pentobarbital anaesthesia blood plasma coagulation parameters have been investigated. to obtain basic values 26 parameters were measured: number of thrombocytes, parameters of thrombelastogram and resonance-thrombogram, prothrombin time, activated partial thromboplastin time, thrombin time, reptilase time, factors i, ii, v, vii, viii, x, antithrombin iii, plasminogen, alpha 1-antitrypsin, alpha 2-antiplasmin, alp ...19968593155
[an outbreak of brucellosis in pigs and conclusions derived on the epidemiology of this animal disease].the examination of an outbreak of brucellosis in domestic pigs showed unambiguously the existence of a natural focus of infection in the population of wild boars. contrary to former assumptions persistence of br. suis, biovar 2, to occur mainly in the population of hares, evidently a change of hosts has taken place. possible cause might be a contrary development of the density of these wild animal populations. health control of wild animals should not be neglected, as it is an important part of ...19958593137
microscopical and ultrastructural studies of the renal artery in domestic swine.both renal arteries from six, 8-month old, camborow hybrid pigs were examined in histological, semithin and ultrathin sections. it was established that the membrana elastica interna showed an expressed degree of wrinkliness. in certain sections, sometimes occupying 20% of the diameter of across-section, its curves were considerably higher and wider than those in the remaining part. the inner elastic membrane in these sections was cleaved in two layers. estates of lengthwise-situated smooth muscl ...19958592976
peculiarities of the connection between mesangial and glomerular endothelial cells in the domestic-swine kidney.the peculiarities of the connection between mesangial and endothelial cells are investigated in material from non-perfused swine kidneys. it is established that cytoplasm fragments of the endothelial cells, located between the mesangial angles, abruptly extend their size, and borders between them are difficult to discern. they possess single microvilli on their lumenal surface, some of which are highly corrugated, branched and fenestrated. in those parts, the mesangial-cell excrescences are tigh ...19958592974
morphological investigations of the glandulae profundae plicae semilunares conjuctivae in the domestic swine (sus scrofa domesticus) and the wild hog (sus scrofa ferus).samples of glandulae profundae plicae semilunares (harderian glands) from five domestic swine and five wild hogs were used for this research. the gland samples were fixed in bouin solution and mounted in paraffin. the paraffin slices were stained with haematoxylin and eosin, according to the periodic-acid-schiff (pas) method, with alcian blue (ph 2.5), with toluidine blue (ph 4.0), and applying a combination of staining with alcian blue (ph 2.5) and the pas method. in domestic swine and wild hog ...19958592973
classical swine fever in wild boar (sus scrofa)--experimental infections and viral persistence.a pregnant wild boar and two wild boar weaners were inoculated intranasally with a field isolate of classical swine fever virus (csfv) recently derived from a diseased domestic pig. the clinical, pathological and haematological findings noted in the young wild boars were comparable to those in domestic weaner pigs inoculated with the same virus isolate. both wild boars showed the acute haemorrhagic form of csf, one animal died 18 days post inoculation (p. i.) and the second one had to be euthani ...19958591736
consequences of selection on muscle composition. a comparative study on gracilis muscle in wild and domestic pigs.european domestic pigs are derived from the european wild pig by genetic selection and differ in their muscular-growth ability. thus, it was the aim of this study to investigate the consequences of genetic selection on muscle composition and fibre size in the gracilis muscle. fibre typing based on the atpase reaction revealed that this muscle in wild pigs is composed mainly of type-i and -iia fibres, whereas, in domestic pigs, type-iib fibres were predominant. for all fibre types, domestic pigs ...19958588708
isolation of encephalomyocarditis virus from dormice (myoxus glis) in italy.two isolates of encephalomyocarditis (emc) virus (zrc 276ra/90 and zrc 292ra/90) were isolated from two dormice (myoxus glis) in tuscany, italy. the two isolates were lethal for laboratory mice and caused a rapid cytopathic effect characterized by rounded and wrinkled cells in both baby hamster kidney cells (bhk21) and african green monkey kidney cells (vero). we found neutralizing antibodies against emc virus in 408 (77%) of 529 domestic pigs (sus scrofa scrofa) and in 165 (49%) of 338 wild boa ...19958583644
sustained-release local hirulog therapy decreases early thrombosis but not neointimal thickening after arterial stenting.adventitial heparin delivery has been shown to inhibit thrombosis and neointimal thickening in a rat carotid injury model. to determine whether sustained, local delivery of hirulog, a potent antithrombin agent, inhibits thrombus formation and neointimal thickening after arterial stenting, silicone polymers containing hirulog were formulated at a concentration of 5.8% by weight and were tested in vitro to determine the rate of drug release. an oversized metallic stent was implanted in the carotid ...19968579010
a closed-chest porcine model of coronary dissection and thrombosis.coronary dissection and thrombosis are important as pathophysiological mechanisms in acute coronary syndromes and as consequences of various interventional procedures. the present study was designed to develop and characterize a simple percutaneous method to produce coronary dissection and thrombosis in the pig.19958574461
augmentation of coronary blood flow by ace inhibition: role of angiotensin and bradykinin.inhibition of the angiotensin converting enzyme (ace) is known to enhance coronary blood flow via partial suppression of angiotensin ii and potentiation of bradykinin. the purpose of these experiments was to evaluate the contribution of each of these mechanisms to the ace inhibition induced changes in blood flow in myocardial regions perfused by intact or stenotic coronary arteries. seven domestic swine were submitted to an 82% stenosis of the left anterior descending artery with the circumflex ...19958556004
effects of dexmedetomidine on coronary hemodynamics and myocardial oxygen balance.alpha 2-adrenergic agonists decrease central sympathetic outflow and maintain normal transmural myocardial blood flow distribution, but intravenous bolus doses of these agents can also induce excessive coronary vasoconstriction and myocardial ischemia. the hypothesis of the present study was that a rapid intravenous bolus of dexmedetomidine, a specific alpha 2-adrenergic agonist, will cause coronary vasoconstriction and accompanying myocardial ischemia in young pigs.19958547552
histomorphologic and histochemical characteristics of carpal glands (glandulae carpeae) in domestic swine (sus scrofa domesticus) and wild swine (sus scrofa ferus).investigations were carried out on samples from carpal glands of domestic and wild swine of different ages. the anatomic position of carpal glands is identical in domestic and wild swine. glandular acini in domestic swine are bigger and more scarce than in wild swine. ldh activity is more intense in wild swine, while the activity of the investigated diaphorases is almost even. the activity alkaline and acid phosphatase is stronger in domestic swine, while the activity of non-specific esterases i ...19958546329
morphological studies of the valves of the kidney vein in domestic swine.it was established macroscopically that 37.5% of the renal veins investigated possess valves. valves were observed on both sides, but predominantly on the left side of the kidneys (14.58%). some valves were also observed in the main branches of the renal veins. the structure of the valves was studied with the use of light and electron microscopy. the most important finding was the observation of mast cells in the valves. this is probably a common biological phenomenon, since mast cells have been ...19958546321
renal venous architectonics in domestic swine.renal venous architectonics were investigated in 240 kidneys from 120 swines at an age of 1-8 months. the methods used were: preparation, corrosion and x-ray examination. it was established that, in 75% of the cases, v. renalis cranialis and v. renalis caudalis were present. in the rest of the cases (25%), v. renalis intermedia was present. it was also observed that right and left renal veins formed distentions, which enveloped the caudal medial part of each adrenal gland. v. adrenalis (supraren ...19958546320
genetic differences in recombination frequency in the pig (sus scrofa).a comparison has been performed on 3 recently published linkage maps of the pig, hereafter designated as the american (a), european (e), and swedish (s) maps. the cumulated distances between common markers in these 3 maps were in the ratio 1.00 (a):0.88 (e):0.77 (s), in keeping with the ratio of the percentages of domestic genome in the reference families used to build the corresponding maps, i.e., 1.00 (a):0.81 (e):0.50 (s). from further recombination frequencies reported in wild boars (in the ...19958536996
pathogenicity of artyfechinostomum oraoni in naturally infected pigs.in an attempt to establish the diarrhoeogenic potential of the newly identified artyfechinostomum oraoni, which was associated with human diarrhoea in a tribal community near calcutta, india, two naturally infected domestic pigs of the locality were followed in captivity. both pigs developed fatal diarrhoea after 5 months. the autopsy revealed a massive infection with the echinostome on a haemorrhagic and oedematous mucosa of the jejunum and duodenum extending up to pyloric end of the stomach. i ...19958525286
mr imaging of gadolinium-dtpa-bma-enhanced reperfused and nonreperfused porcine myocardial infarction.to investigate whether gd-dtpa-bma-enhanced mr imaging permits differentiation between reperfused and nonreperfused myocardial infarction, myocardial infarction was induced in 12 domestic pigs. in 6 pigs, gd-dtpa-bma, 0.3 mmol/kg b.w. was administered i.v. 60 min after the occlusion. in 6 other pigs, the infarctions were reperfused 80 min after the occlusion, followed by injection of gd-dtpa-bma after 20 min of reperfusion. radiolabeled microspheres were used to confirm zero-flow during the occl ...19958519575
detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure.a total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid dna and specific tetracycline resistance determinants. more than 84% of the isolates harbored plasmid dna, which ranged in size from 2.1 to 186 kb. a total of 78 isolates (68.4%) were resistant to tetracycline at concentrations greater than 4 micrograms/ml. plasmid dnas from about 56% of the tetracycline-resistant isolates hybr ...19938517740
[the distribution and biology of capillaria garfiai from sus scrofa].the infestation extent of wild boars (sus scrofa) with capillaria garfiai gallégo & mas-coma, 1975 in east-austria and the localisation of the nematodes in the tongue (n = 159) were examined. the infestation extent (prevalence) was between 66.6 and 90.9 percent. concerning the location of the parasites in the tongue there was a striking difference between c. garfiai males and females. so, the number of c. garfiai females continuously decreased from the first to the fourth quarter of the tongue, ...19938508219
intramyocardial hypercarbic acidosis during cardiac arrest and resuscitation.to define changes in intramyocardial ph and pco2 during cardiac arrest and resuscitation.19938504660
zygotene-pachytene substaging and synaptonemal complex karyotyping of boar spermatocytes.synaptonemal complex analysis, by electron microscopy of spread spermatocytes after phosphotungstic acid staining, made possible description of the chromosomal synaptic patterns and the synaptonemal complex karyotype of the pig (sus scrofa domestica). the autosomal synaptic pattern in conjunction with the sex chromosome morphology and pairing behaviour can serve as a reference for the meiotic cell progression from the zygotene stage to the pachytene. the autosomes started terminal synapsis at ea ...19938478210
modulation of transforming growth factor-beta 1 stimulated elastin and collagen production and proliferation in porcine vascular smooth muscle cells and skin fibroblasts by basic fibroblast growth factor, transforming growth factor-alpha, and insulin-like growth factor-i.during tissue repair and development, matrix accumulation is modulated as multiple signals impinge on target cells. we have investigated the effects of combinations of the mitogenic cytokines, basic fibroblast growth factor (bfgf), transforming growth factor alpha (tgf-alpha), and insulin-like growth factor-1 (igf-1) with transforming growth factor-beta 1 (tgf-beta 1) with respect to the production of two matrix components, elastin and type i collagen. using specific enzyme-linked immunoassays f ...19938468360
characterization of the apolipoprotein ai and ciii genes in the domestic pig.the apolipoproteins (apo) ai and ciii are important constituents of triglyceride-rich lipoproteins and high-density lipoproteins. in humans, apo ai is believed to play an important protective role in the pathogenesis of arteriosclerosis, whereas apo ciii might be involved in the development of hypertriglyceridemia. both human genes are located within a gene cluster on chromosome 11. although the domestic pig has been widely used as an animal model in arteriosclerosis and lipid research, the porc ...19938468059
coronary pressure-flow regulation in the stunned myocardium.in this study, we used a swine model to study coronary autoregulation in the stunned myocardium. in 18 domestic swine, the left anterior descending coronary artery was cannulated and flow to this artery controlled via an extracorporeal perfusion circuit. stunning was induced by reducing pump flow to approximately 25% of the baseline value for 15 minutes followed by 1 hour of reperfusion. this ischemia/reperfusion protocol reduced systolic shortening to approximately 50% of control, at 1 hour of ...19938461520
effects of r-pia, a selective a1 adenosine agonist, on haemodynamics and ischaemic arrhythmias in pigs.adenosine has previously been shown to protect against ischaemia induced ventricular arrhythmias. the aim of this study was to determine whether stimulation of a1 adenosine receptors with a selective agonist also protects against arrhythmias, and to attempt to elucidate the underlying mechanisms.19938458036
ligation of the renal pedicle during laparoscopic nephrectomy: a comparison of staples, clips, and sutures.evaluation of the role of staples, clips, and sutures for laparoscopic ligation of the renal artery revealed that occluding the renal artery with three, 9 mm titanium clips is as secure as occluding the renal artery with standard 2-0 and 0-silk ligatures. however, a triple staggered line of 2.5 mm staples placed across the renal artery was not as secure as either clips or silk sutures. the authors also studied eight female farm pigs who underwent laparoscopic nephroureterectomy with en masse sta ...19938453134
plasma levels of several androgens and estrogens from birth to puberty in male domestic pigs.plasma concentrations for several androgens and estrogens were determined in male domestic pigs from birth to eight months of age. samples (n = 6) of blood were collected from yorkshire males at weekly intervals from birth to four weeks, and thereafter at monthly intervals to eight months. radioimmunoassays were done without extraction from plasma for dehydroepiandrosterone sulphate, androstenedione and estrone sulphate. other steroids were measured after solid-phase extraction, separate elution ...19938451912
changes in levels of mrna encoding myosin heavy chain in porcine trachealis during ontogenesis.we determined the steady-state level of mrna for myosin heavy chain (mhc) from airway smooth muscle during maturation in domestic swine. tissues were excised, and airway smooth muscle was dissected from three neonatal (neo), three 2-wk-old swine (2ws), three 10-wk-old swine (10ws), and three adult swine. total rna was isolated, fractionated, and transferred to a nitrocellulose membrane (northern blot). a single-stranded oligonucleotide of 63-nt was synthesized corresponding to the 3' coding regi ...19938448016
laparoscopic gastrointestinal anastomoses.in a preliminary study of laparoscopic gastrointestinal anastomosis, a gastric outlet obstruction was created under laparoscopic control in six domestic pigs, weighing an average of 25 kg. a simultaneous gastroenterostomy was formed with an end-stapling device. all animals recovered clinically, and good anastomotic healing was documented 1 week postoperatively. the authors suggest that an end-stapling device could be one method of creating a gastrointestinal anastomosis for humans that is safe a ...19938443722
effects of graded doses of epinephrine on both noninvasive and invasive measures of myocardial perfusion and blood flow during cardiopulmonary resuscitation.epinephrine administered during cardiopulmonary resuscitation (cpr) is known to increase aortic diastolic and myocardial perfusion pressures, while enhancing myocardial blood flow. optimal dosing of epinephrine during cpr is less certain. interest in high-dose epinephrine use under such circumstances is increasing. the effect of different doses of epinephrine on simultaneously measured perfusion pressures, myocardial blood flow, cardiac output, and end-tidal co2 (pco2) (used as an indirect measu ...19938440112
myocardial hypercarbic acidosis reduces cardiac resuscitability.the severity of spontaneous myocardial hypercarbic acidosis during cardiac arrest previously has been predictive of the likelihood of restoring spontaneous circulation. the present study investigated whether hypercarbia itself impairs cardiac resuscitation. since coronary perfusion pressure is the overriding determinant of cardiac resuscitability, we used a porcine model of cardiac arrest in which coronary perfusion pressure was controlled.19938439030
analysis of aphidicolin-induced chromosome fragility in the domestic pig (sus scrofa).aphidicolin (apc)-sensitive fragile sites were identified in chromosome preparations from peripheral lymphocyte cultures of 12 four-way crossbred pigs. a chi 2 analysis demonstrated that aphidicolin-induced breakage events and previously reported in vivo chromosome rearrangement events were not independent. comparison of expected poisson and negative binomial distributions to observed breakage patterns indicated that the negative binomial distribution provided a better fit to experimental data. ...19938428508
augmented efficacy of external cpr by intermittent occlusion of the ascending aorta.after prolonged cardiac arrest, conventional methods of closed-chest cardiac compression are ineffective. this is primarily because of failure to generate minimal threshold levels of coronary perfusion pressure for cardiac resuscitation. this report introduces a new option for cardiac resuscitation by use of a combination of intermittent ascending aortic balloon occlusion, aortic infusion, and precordial compression to increase the pressure gradient for coronary perfusion.19938403337
application of human minisatellite probes to the development of informative dna fingerprints and the isolation of locus-specific markers in animals.in this study, the alpha-globin 3' hvr (jarman et al., 1986), the rna transcripts of 33.15 and 33.6 (carter et al., 1989), and the human locus-specific minisatellites ms1, ms8, ms31, ms32 (wong et al., 1987), ms51, ms228a (armour et al., 1989) and g3 (wong et al., 1986) were applied to domestic pigs, common marmoset monkeys (callithrix jacchus jacchus) and waldrapp ibises (geronticus eremita) and evaluated for their suitability firstly for isolating polymorphic vntr markers from genomic librarie ...19938400710
prevalence of pseudorabies (aujeszky's disease) virus antibodies in feral swine in florida.serum samples collected from feral swine (sus scrofa) throughout florida (usa) from 1980 to 1989 were tested for antibodies to pseudorabies virus (prv) by the serum neutralization test, the latex agglutination test, or by the enzyme-linked immunosorbent assay. seropositive swine were detected at 11 of 13 sites with a composite seroprevalence of 34.8% (579 of 1,662 samples; range = 5.9% to 58.2%) for sites with seropositive swine. data on age and sex of the swine were available from three sites. ...19938394943
proof of alkaline phosphodiesterase i as a phosphatidylinositol-anchor enzyme.1. ectoenzyme release from kidney brush border membranes of rattus norvegicus and sus scrofa domesticus by phosphatidylinositol-specific phospholipase c (piplc) of bacillus thuringiensis was studied. 2. the levels of specific activities of ectoenzymes in r. norvegicus kidney brush border membranes were higher than those in s. scrofa domesticus. about 10-fold higher values were found for specific activities of alkaline phosphatase and gamma-glutamyl transpeptidase in r. norvegicus. 3. alkaline ph ...19938394252
reduction of systemic drug exposure after hepatic arterial infusion of doxorubicin with complete hepatic venous isolation and extracorporeal chemofiltration.hepatic arterial infusion of doxorubicin has produced tumor response in hepatic malignancies; however, the limited success of these treatments is related in part to dose-limiting systemic toxicities. the purpose of this study was to determine whether a novel venous isolation-chemofiltration system could limit systemic exposure to doxorubicin after hepatic arterial infusion.19938367814
the pathophysiologic changes following bile aspiration in a porcine lung model.aspiration of bile is an underpublicized aspiration syndrome. using a porcine lung model, the physiologic response and the histopathology of lung tissue were evaluated after the intratracheal instillation of sublethal doses of bile. twenty-one domestic swine (11 to 19 kg) were the studied population. three groups of five swine were evaluated: a control group received intratracheal physiologic saline (ph 7.45); study group 1 received strained gastric contents (ph 2.24); and study group 2 received ...19938365309
protective effect of ribcys following high-dose irradiation of the rectosigmoid.ribose-cysteine (ribcys) is a prodrug of l-cysteine that stimulates glutathione biosynthesis. increased glutathione levels have been shown to have a protective effect against radiation-induced injury and oxidative stress. surface oximetry has previously been used successfully to predict anastomotic leakage.19938348853
dual contrast enhancement of both t1- and t2-weighted sequences using ultrasmall superparamagnetic iron oxide.bms 180549 (previously ami-227), an ultrasmall superparamagnetic iron particulate agent, was investigated to determine its utility as a contrast agent on t1-weighted, as well as t2-weighted sequences, as a function of route of administration, (intravenous versus selective arterial) and concentration. twelve farm pigs were divided into three groups of four each by route of administration (intravenous, selective superior mesenteric, or selective hepatic arterial injection). 10 mumol/kg and 20 mumo ...19938345779
microsatellite repeats in pig (sus domestica) and chicken (gallus domesticus) genomes.separate databases were made for all pig and chicken sequences in the genbank database, which were then systematically searched for all possible mononucleotide, dinucleotide, trinucleotide, and tetranucleotide repeats of about 20 bp or greater in length. a total of 13% of pig genes and 10% of chicken genes contained one or more of these microsatellite-like repeats. there was a considerable difference in the frequency of dinucleotide repeats between pigs, where they comprised 30% of the repeats f ...19938340616
probucol decreases neointimal formation in a swine model of coronary artery balloon injury. a possible role for antioxidants in restenosis.restenosis after percutaneous transluminal coronary angioplasty is the major limitation of the long-term success of this procedure. the process of restenosis is similar to an accelerated form of atherosclerosis. thus, therapeutic interventions that limit the progression and initiation of atherosclerosis may be beneficial in the treatment of restenosis. one such intervention is the antioxidant drug probucol, which has demonstrated benefit in animal models of atherosclerosis.19938339427
effects on wound healing of zinc oxide in a hydrocolloid dressing.zinc oxide incorporated in gauze enhances healing of chronic wounds in humans and experimental pig wounds.19938335742
secretion of 19-hydroxyandrostenedione and 19-hydroxytestosterone by porcine leydig cells in vitro and in vivo.19-hydroxytestosterone and 19-hydroxyandrostenedione have been identified as secretory products of the testes in the mature male domestic pig. their isolation and identification were made by reverse-phase high-performance liquid chromatography and capillary gas chromatography-mass spectrometry (cgc-ms) of extracts from testicular vein blood and media of incubations with leydig cells. blood was collected from veins on the surface of the testes of anaesthetized boars. collagenase-dispersed percoll ...19938326254
preconditioning improves energy metabolism during reperfusion but does not attenuate myocardial stunning in porcine hearts.it has been reported that a brief period of coronary occlusion and reperfusion slows the rate of atp depletion during subsequent sustained ischemia as well as limiting infarct size. however, it has not yet been determined whether ischemic preconditioning also has an effect on the functional and metabolic recovery of stunned myocardium. our study was designed to address this problem.19938319337
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