Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| stimulation of immunity in indian major carp catla catla with herbal feed ingredients. | catla catla, catla (150 +/- 20 g) were fed a diet containing seed of achyranthes aspera (0.5%) and control diet without a. aspera for four weeks prior to and after ip injection with chicken erythrocytes. fish were sampled for four consecutive weeks after immunization. hemagglutination antibody titers were significantly higher in the test group of fishes compared with the control group. serum globulin levels were significantly (p(t-test) < 0.05) higher in the test group than control group on days ... | 2005 | 15561562 |
| [cloning and expression analysis in mature individuals of salmon gonadotropin-releasing hormone (sgnrh) gene in common carp]. | two types of complementary dnas (cdna) encoding the precursor of salmon gonadotropin-releasing hormone (sgnrh, [trp7, leu8] gnrh) are cloned and sequenced from common carp brain using rapid amplification of cdna ends (race). the two cdnas are referred to sgnrh cdna1 and cdna2, and the full-length fragment of cdna1 and cdna2 were 393 and 478 bp, respectively. two sgnrh cdnas contain an open reading frame of 285 bp, which encodes the sgnrh precursor including 94 amino acid residues. the sgnrh prec ... | 2004 | 15552041 |
| coping with cold: an integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate. | how do organisms respond adaptively to environmental stress? although some gene-specific responses have been explored, others remain to be identified, and there is a very poor understanding of the system-wide integration of response, particularly in complex, multitissue animals. here, we adopt a transcript screening approach to explore the mechanisms underpinning a major, whole-body phenotypic transition in a vertebrate animal that naturally experiences extreme environmental stress. carp were ex ... | 2004 | 15550548 |
| temperature and the expression of myogenic regulatory factors (mrfs) and myosin heavy chain isoforms during embryogenesis in the common carp cyprinus carpio l. | embryos of the common carp, cyprinus carpio l., were reared from fertilization of the eggs to inflation of the swim bladder in the larval stage at 18 and 25 degrees c. crna probes were used to detect transcripts of the myogenic regulatory factors myod, myf-5 and myogenin, and five myosin heavy chain (myhc) isoforms during development. the genes encoding myf-5 and myod were switched on first in the unsegmented mesoderm, followed by myogenin as the somites developed. myf-5 and myod transcripts wer ... | 2004 | 15531645 |
| subchronic oral toxicity of microcystin in common carp (cyprinus carpio l.) exposed to microcystis under laboratory conditions. | the subchronic oral toxicity of microcystin in common carp (cyprinus carpio l.) was investigated in this study. the fish (mean body weight of 322+/-36 g, n=10) were orally exposed to microcystis by feeding with bloom scum at a dose of 50 microg microcystins/kg body weight under laboratory conditions for 28 days. growth assay results showed that microcystin could completely inhibit the growth of carp, but failed to change the fish hepatosomatic index. ultrastructural examination by electron micro ... | 2004 | 15530963 |
| comparative and evolutionary analysis of the cytochrome b sequences in cyprinids with different ploidy levels derived from crosses. | the mitochondrial cyt b genes in the allotetraploid and triploid crucian carp as well as triploid common carp were isolated and completely sequenced. their dna sequences were compared with those derived from the cyt b genes of the red crucian carp, japanese crucian carp, and common carp with mega 1.0 software. phylogenetic analysis revealed the sister relationships between allotetraploid and diploid red crucian carp, between the triploid crucian carp and diploid japanese crucian carp, and betwee ... | 2004 | 15521428 |
| concentrations of a koi herpesvirus (khv) in tissues of experimentally infected cyprinus carpio koi as assessed by real-time taqman pcr. | the koi herpesvirus (khv) is a herpes-like virus now recognized as a worldwide cause of mortality among populations of koi cyprinus carpio koi and common carp cyprinus carpio carpio. temperature is a key factor influencing virus replication both in cell culture and in the tissues of experimentally infected fish. genomic dna sequences were used to optimize a rapid real-time taqman pcr assay to detect and quantify khv dna as found in the tissues of virus-exposed fish. the assay allowed analytical ... | 2004 | 15521316 |
| protection against experimental aeromonas salmonicida infection in carp by oral immunisation with bacterial antigen entrapped liposomes. | liposome-entrapped atypical aeromonas salmonicida antigen was prepared to investigate the potential protective efficacy for a. salmonicida infection. carp (cyprinus carpio) were immunised orally with liposome-entrapped a. salmonicida antigen. after immunisation, significantly higher antigen-specific antibodies were detected in serum, intestinal mucus and bile than non-immunised control group. furthermore, immunised carp were challenged by immersion with 1 x 10(6) cfu ml(-1) of a. salmonicida for ... | 2005 | 15519542 |
| disparate opinions regarding indications for coronary artery revascularization before elective vascular surgery. | despite consensus guidelines, the optimal strategy for preoperative cardiac risk management among patients scheduled for major noncardiac surgery remains controversial. this study assesses current opinion about the role of preoperative coronary revascularization for patients with coronary artery disease scheduled for elective vascular surgery. thirty-one practicing cardiologists recruited from 4 different regions reviewed case records, imaging tests, and coronary angiograms of 12 patients with c ... | 2004 | 15518605 |
| determination of a bound musk xylene metabolite in carp hemoglobin as a biomarker of exposure by gas chromatography-mass spectrometry using selected ion monitoring. | musk xylene (mx) is widely used as a fragrance ingredient in commercial toiletries. identification and quantitation of a bound 4-amino-mx (amx) metabolite was carried out by gas chromatography-mass spectrometry (gc-ms) with selected ion monitoring (sim). detection of amx occurred after the cysteine adducts in carp hemoglobin (hb), derived from the nitroso metabolite, were released by alkaline hydrolysis. the released amx metabolite was extracted into n-hexane. the extract was preconcentrated by ... | 2004 | 15516317 |
| [studies on the ploymorphic of sperm of f2 hybrids of red crucian carp x common carp]. | athe ultrastructures of the sperm of f2 hybrids of red crucian carp x common carp were studied by using scanning and transmission electron microscope. the sperm of the f2 hybrids consisted of head, mid-piece and tail. there was no acrosome at the anterior end of the nuclears, whereas there was a vesicle. the results revealed that there existed obviously ploymorphic in the sperm of f2 hybrids. in the water-like semen from males of f2 hybrids, different sizes of the head of the sperm including hap ... | 2004 | 15511069 |
| fumonisin b1 causes multiple lesions in common carp (cyprinus carpio). | year-1 carp were fed ratios containing 100mg/kg and 10 mg/kg of added fumonisin b1 for 42 days. the experimental and control fish were examined clinically during the experiment and at the end all fish were necropsied and histological changes recorded. blood vessels, liver, exocrine and endocrine pancreas, excretory and haematopoietic kidney, heart and brain were sensitive both to 100 and 10mg/kg of fb1 in the diet and the rodlet cell (rc) frequency was considerably increased in and around damage ... | 2004 | 15503536 |
| mycobacterium marinum strains can be divided into two distinct types based on genetic diversity and virulence. | mycobacterium marinum causes a systemic tuberculosis-like disease in a large number of poikilothermic animals and is used as a model for mycobacterial pathogenesis. in the present study, we infected zebra fish (danio rerio) with different strains of m. marinum to determine the variation in pathogenicity. depending on the m. marinum isolate, the fish developed an acute or chronic disease. acute disease was characterized by uncontrolled growth of the pathogen and death of all animals within 16 day ... | 2004 | 15501758 |
| carbonic anhydrases: current state of the art, therapeutic applications and future prospects. | carbonic anhydrases (cas, ec 4.2.1.1) are wide-spread enzymes, present in mammals in at least 14 different isoforms. some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii, ca xiii), others are membrane-bound (ca iv, ca ix, ca xii and ca xiv), ca v is mitochondrial and ca vi is secreted in the saliva and milk. three cytosolic acatalytic forms are also known (carp viii, carp x and carp xi). the catalytically active isoforms, which play important physiological and patho-physiological fu ... | 2004 | 15499993 |
| impact of some chlorinated pesticides on the haematology of the fish cyprinus carpio and puntius ticto. | the effects of pesticides on blood characteristics and histological changes in erythrocytes of the fish species cyprinus carpio and puntius ticto were studied. the fishes were exposed to sub lethal concentrations of different chlorinated pesticides namely aldrin, dieldrin, ddt, bhc and chlordane for 10, 20 and 30 d in continuous flow-through test. the lc50 values were calculated based on acute toxicity tests and the sublethal doses were arrived at for chronic bioassay studies. results showed an ... | 2004 | 15495970 |
| cloning and expression analysis in mature individuals of two chicken type-ii gnrh (cgnrh-ii) genes in common carp (cyprinus carpio). | gonadotropin-releasing hormone (gnrh) is a conservative neurodecapeptide family, which plays a crucial role in regulating the gonad development and in controlling the final sexual maturation in vertebrate. two differing cgnrh-ii cdnas of common carp, namely cgnrh-ii cdna1 and cdna2, were firstly cloned from the brain by rapid amplification of cdna end (race) and reverse transcription- polymerase chain reaction (rt-pcr). the length of cgnrh-ii cdna1 and cdna2 was 622 and 578 base pairs (bp), resp ... | 2004 | 15493476 |
| [polymorphism in intron 2 of crucian carp gh i gene]. | high length and nucleotide polymorphisms in intron2 of gh i gene were detected in 162 individuals,which were from seven wild crucian carp colonies, two goldfish colonies and one fangzheng crucian carp colony. using denaturating polyareylamide gel electrophoresis (dpage) and single-strand conformation polymorphism (sscp), seven length variants and 15 haplotypes were identified in these fishes. the length types and haplotypes diversity was 4.32% and 9.26%, respectively. sequence analysis of the 15 ... | 2004 | 15490876 |
| detection of koi herpesvirus in common carp, cyprinus carpio l., by loop-mediated isothermal amplification. | loop-mediated isothermal amplification (lamp) is a novel method that amplifies dna with high specificity and rapidity under isothermal conditions. in this study, using the lamp method, a protocol for koi herpes virus (khv) detection in common carp was designed. a set of four primers, two inner and two outer, were designed based on the sequence of the thymidine kinase (tk) gene of khv. time and temperature conditions for detection of khv were optimized for 60 min at 65 degrees c. the detection li ... | 2004 | 15482423 |
| [karyotypic analysis from two clones of gynogenetic pengze crucian carp (carassius auratus of pengze)]. | pengze crucian carp (carassius auratus of pengze), one of the most popular cultural fishes in china, is an endemic bisexual population with natural gynogenetic reproduction mode. just like silver crucian carp, investigation on the karyotype of pengze crucian carp is important to understanding the diversity and the evolution of the unisexual vertebrates. two distinguishable gynogenetic clones of pengze crucian carp, named clone h and clone l, respectively,were detected recently. in this study, th ... | 2004 | 15481531 |
| three novel carp cxc chemokines are expressed early in ontogeny and at nonimmune sites. | three novel cxc chemokines were identified in common carp (cyprinus carpio l.) through homology cloning. phylogenetic analyses show that one of the three cxc chemokines is an unambiguous orthologue of cxcl14, whereas both others are orthologues of cxcl12, and were named cxcl12a and cxcl12b. percentages of amino acid identity between each of these carp chemokines and their human and mouse orthologues are markedly higher than those reported previously for other carp cxc chemokines, suggestive of i ... | 2004 | 15479238 |
| use of a complex approach for assessment of metamizole sodium and acetylsalicylic acid toxicity, genotoxicity and cytotoxicity. | a complex approach based on the use of test organisms belonging to different systematic groups (plants, invertebrates and vertebrates), as well as the nucleolar biomarker and the micronucleus test on their cells, was applied to assess the toxicity, cytotoxicity and genotoxicity of two pharmaceutical substances (metamizole sodium and acetylsalicylic acid) applied at ic(50) concentrations for mammalian cells. the compound acetylsalicylic acid was evaluated at a concentration (1.6 x 10(3) mg l(-1)) ... | 2015 | 15478170 |
| analyses of impact of metal ion contamination on carp (cyprinus carpio l.) gill cell suspensions. | the decline in fish population because of water contamination is problem. as a result of direct exposure in water, it has been readily accepted that the gills are the main site of water contamination and toxicity (e.g., metal ions). in the present study, we investigated metal ion contamination on the functional capacity of carp gill cells with antioxidant interactions in an in vitro study. the extent of cellular membrane damage, lipid peroxidation (lpo) (as tbars levels), and glutathione (gsh) c ... | 2004 | 15475620 |
| cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp (cyprinus carpio) nuclei and goldfish (carassius auratus) enucleated eggs. | in previous studies of nuclear transplantation, most cloned animals were obtained by intraspecies nuclear transfer and are phenotypically identical to their nuclear donors; furthermore, there was no further report on successful fish cloning since the report of cloned zebrafish. here we report the production of seven cross-genus cloned fish by transferring nuclei from transgenic common carp into enucleated eggs of goldfish. nuclear genomes of the cloned fish were exclusively derived from the nucl ... | 2005 | 15469998 |
| [triploid crucain carp-allotetraploid hybrids (male) x goldfish( hermaphrodite)]. | a new type of triploid crucian carp was generated by mating the males of the allotetraploid hybrids of red crucian carp (carassius auratus red var.) (female) x common carp (cyprinus carpio l.) (male), with the females the goldfish( carassius auratus red var.) which was red in color and possessed the biforked tails. the biological characteristics of one-year old triploid crucian carp including the number and karotype of chromosome, gonadosomatic indices and gonadal structures, appearance and grow ... | 2004 | 15468916 |
| characterization and comparison of fatty acyl delta6 desaturase cdnas from freshwater and marine teleost fish species. | fish are the most important dietary source of the n-3 highly unsaturated fatty acids (hufa), eicosapentaenoic (epa) and docosahexaenoic acid (dha), that have particularly important roles in human nutrition reflecting their roles in critical physiological processes. the objective of the study described here was to clone, functionally characterize and compare expressed fatty acid desaturase genes involved in the production of epa and dha in freshwater and marine teleost fish species. putative fatt ... | 2004 | 15465674 |
| myxosporea: a new look at long-known parasites of fish. | with the identification of myxosporea as agents of swimbladder inflammation (sbi) in carp, and proliferative kidney disease (pkd) in salmonids, this group of over 1000 protistan species has been receiving renewed attention. almost all species are parasites offish, and some can cause serious losses in commercial fish farms. yet adequate control measures cannot be planned without clarification of basic features of the parasites' life cycles and modes of transmission. in this article, jiri lom revi ... | 1987 | 15462878 |
| [contamination source identification study of organochlorines pesticides in 2000 chinese total diet study]. | the third chinese total diet study to estimate dietary intake of contaminants was carried out in 2000. the levels of hch and ddt residues were less significant in most of foodstuffs, but the level of hch residues of aquatic foods in south 2 and in north 1 is respectively 75.99microg/kg and 170.72microg/kg, and the ratio of gamma-hch is 90% of the total hchs residues. to identify the contamination source of gamma-hch, the individual province composite samples and the individual samples of aquatic ... | 2004 | 15461282 |
| uptake of sediment-bound bioavailable polychlorobiphenyls by benthivorous carp (cyprinus carpio). | it is unclear whether accumulation of sediment-bound chemicals in benthivorous fish depends on the degree of sequestration in the sediment like it does for invertebrates. here, we report on the potential of slow and fast desorbing sediment-bound polychlorobiphenyl (pcb) fractions for accumulation in carp (cyprinus carpio) in lake enclosures treated with different nutrient doses. routes of pcb uptake were quantitatively evaluated for 15 pcbs (log kow range 5.6-7.8) using model analysis. fast-deso ... | 2004 | 15461156 |
| response of hexokinase enzymes and the insulin system to dietary carbohydrates in the common carp, cyprinus carpio. | the response of the common carp to diets with varying amounts of digestible starch, provided either as pea meal (lp, hp, 30 and 46% peas, respectively) or as cereal (lw, hw, 30 and 46% wheat, respectively), was studied and compared with the response to a carbohydrate-free protein-rich diet (cf). here we focused on the utilisation of dietary carbohydrates by examining the relationship between dietary starch intake, hepatic hexokinase activities, circulating insulin and muscle insulin receptor sys ... | 2011 | 15460162 |
| maintained cardiac pumping in anoxic crucian carp. | like most vertebrates, humans die within minutes when deprived of molecular oxygen (anoxia), in part because of cardiac failure. in contrast, some freshwater turtles can survive anoxia for months at low temperatures, but to do so, they drastically suppress cardiac activity and autonomic cardiovascular control. although carassius carassius, the crucian carp, shares this anoxia tolerance, we show that it has a unique ability among vertebrates to retain normal cardiac performance and autonomic card ... | 2004 | 15459381 |
| first record of contracaecum spp. (nematoda: anisakidae) in fish-eating birds from zimbabwe. | endoparasites of fish-eating birds, phalacrocorax africanus, p. carbo, anhinga melanogaster and ardea cinerea collected from lake chivero near harare, zimbabwe, were investigated. adult contracaecum spp. were found in the gastrointestinal tract (prevalence 100 % in p. africanus, p. carbo and a. melanogaster; 25 % in a. cinerea). parasite intensity was 11-24 (mean 19) in p. africanus, 4-10 (mean 7) in p. carbo, 4-56 (mean 30) in a. melanogaster and 2 (mean 0.5) in a. cinerea. the cormorants fed m ... | 2004 | 15456162 |
| carbonic anhydrase-related protein viii: autoantigen in paraneoplastic cerebellar degeneration. | serum of a patient with paraneoplastic cerebellar degeneration (pcd) and malignant melanoma had a unique reactivity with purkinje cells that was not observed in serum of patients with pcd and other tumors, or with malignant melanoma without paraneoplastic syndromes. the screening of a human cerebellar complementary dna expression library with the patient's serum resulted in the isolation of the ca8 gene. ca8 encodes carbonic anhydrase-related protein (carp) viii, preferentially expressed in purk ... | 2004 | 15389893 |
| calcium-binding protein caldendrin and camkii are localized in spinules of the carp retina. | calcium-binding proteins translate the influx of ca(2+) at excitatory synapses into spatiotemporal signals that regulate a variety of processes underlying synaptic plasticity. in the fish retina, the synaptic connectivity between photoreceptors and horizontal cells undergoes a remarkable plasticity, triggered by the ambient light conditions. with increasing light, the synaptic dendrites of horizontal cells form numerous spinules that are dissolved during dark adaptation. the dynamic regulation o ... | 2004 | 15389610 |
| loss of filament-forming ability of myosin by non-enzymatic glycosylation and its molecular mechanism. | carp and scallop myosin and their subfragments (s-1 and rod) were reacted with glucose to investigate the effect of non-enzymatic glycosylation on the functionality of myosin. the filament-forming ability of the myosin rod diminished with the progress of non-enzymatic glycosylation and myosin became soluble in 0.1 m nacl. the inhibition of the self-assembly of myosin molecules occurred chemically as a result of the increase in negative charge repulsion among myosin molecules and, further, physic ... | 2004 | 15388325 |
| immunology-related perturbations induced by copper and chitosan in carp (cyprinus carpio l.). | copper is used in treatment mixtures to control fungal diseases in vineyards. its concentrations are relatively high in some aquatic ecosystems, and the main problem observed in this study was the antioxidant stress induced by this heavy metal. copper toxicologic effects in aquatic organisms have prompted the demand for alternative use of low-toxicity molecules in culture treatments. chitosan is a polymer with antifungal property similar to copper and may be an interesting biopesticide. thus, it ... | 2004 | 15386131 |
| immunotoxicity of pentachlorophenol on macrophage immunity and igm secretion of the crucian carp (carassius auratus). | 2004 | 15386086 | |
| characterization of retinoic acid neuromodulation in the carp retina. | visual sensation in vertebrates starts with the isomerization of 11-cis retinaldehyde into all-trans retinaldehyde. aldehyde dehydrogenases, present in the pigment epithelium and some retinal cells, convert all-trans retinaldehyde into all-trans retinoic acid (at-ra). evidence in the retina and the hippocampus has accumulated, showing that at-ra, besides being a morphogenetic factor, also acts as a neuromodulator. in mature retina, at-ra affects visual processing by acting on gap junctional cond ... | 2004 | 15378613 |
| determination of polychlorinated dibenzo-p-dioxins and dibenzofurans and comparison of extraction methods for edible freshwater fish and frogs in south korea by using a high-resolution gc/ms. | three different extraction methods were evaluated: soxhlet extraction, accelerated solvent extractor extraction and alkali digestion with high-resolution gas chromatography/high-resolution mass spectrometry, for the analysis of edible freshwater fish and frogs. the recovery efficiencies for the different extraction methods were also compared. using these extraction methods, the concentrations and spatial distributions of these compounds in freshwater fish and frogs (n = 99), collected from 31 lo ... | 2004 | 15370843 |
| homing of gyrodactylus salaris and g. derjavini (monogenea) on different hosts and response post-attachment. | in natural european waters, the congeneric monogeneans gyrodactylus derjavini mikailov, 1975 and g. salaris malmberg, 1957 are primarily found on brown trout salmo trutta l. and atlantic salmon salmo salar l., respectively. interestingly, rainbow trout, oncorhynchus mykiss (walbaum), originating from north america, is as susceptible as brown trout to g. derjavini. however, the mechanisms involved in this host specificity are poorly understood but may include behavioural, mechanical and chemical ... | 2004 | 15357405 |
| some immune parameters in carp cyprinus carpio susceptible and resistant to the haemoflagellate trypanoplasma borreli. | the present study addresses aspects of the (specific) immune response of carp to the haemoflagellate trypanoplasma borreli. sera of resistant carp contained antibodies, which agglutinated the flagellates in vitro. when flagellates were incubated in fish sera from resistant carp, binding of antibodies to flagellates could be demonstrated by flow cytometry, and t. borreli were effectively killed. heat-treatment of the sera prevented killing, indicating that complement activation is important for t ... | 2004 | 15352523 |
| dietary accumulation and metabolism of polybrominated diphenyl ethers by juvenile carp (cyprinus carpio). | polybrominated diphenyl ethers (pbdes) are hydrophobic organic contaminants with properties and nomenclature similar to polychlorinated biphenyls (pcbs). while much information is available on the bioaccumulation and pharamcokinetics of pcbs, little information is available on pbdes. in this study, juvenile carp were exposed to a diet spiked with a cocktail of four bde congeners (2,4,4'-tribromode [bde 28], 2,2',4,4'-tetrabromode [bde 47], 2,2',4,4',5-pentabromode [bde 99], and 2,2',4,4',5,5'hex ... | 2004 | 15352483 |
| xenobiotic substances such as pcb mixtures (aroclor 1254) and tbt can influence swimming behavior and biotransformation activity (gst) of carp (cyprinus carpio). | different groups of carp were treated with polychlorinated biphenyl (pcb) or tributyltin (tbt), and possible effects of the chemicals on the swimming behavior of the carp were examined using the behavioquant system. by evaluating quantitative behavioral parameters of the animals, it became evident that exposure to high concentrations of chemicals (organotin, 7 microg l(-1), or polychlorinated biphenyl, 22 microg l(-1)) severely affected the carp, causing a significant change in their swimming sp ... | 2004 | 15352262 |
| the distribution of osteocalcin, degree of mineralization, and mechanical properties along the length of cyprinus carpio rib bone. | this study examined the spatial distribution of selected biochemical and mechanical properties along the length of carp rib bone. carp rib bone was chosen because of its unusually high osteocalcin content relative to other extractable proteins. the amount of osteocalcin was significantly lower (p<0.01) at the most distal section, relative to all other sections. the amount of phosphate (p<0.05) and the elastic modulus in the longitudinal plane (p<0.0001) were found to be significantly higher in t ... | 2001 | 15348241 |
| [genetic analysis on diversity of reproductive modes of silver crucian carp inferred by transferrin and isozyme markers]. | silver crucian carp (carassius auratus gibelio) has been known for its gynogenetic reproduction. in this paper, eggs from clone f of gynogenetic silver crucian carp were inseminated respectively by sperms of clone d, clone a and common carp, and polymorphic patterns of transferrin and isozymes of the produced three offspring fd, fa and fl were studied comparatively to explore the diversity in reproductive modes of silver crucian carp. as control, zymograms of fl progenies exhibited a maternally ... | 2002 | 15346982 |
| evolution of vertebrate genes related to prion and shadoo proteins--clues from comparative genomic analysis. | recent findings of new genes in fish related to the prion protein (prp) gene prnp, including our recent report of sprn coding for shadoo (sho) protein found also in mammals, raise issues of their function and evolution. here we report additional novel fish genes found in public databases, including a duplicated sprn gene, sprnb, in fugu, tetraodon, carp, and zebrafish encoding the sho2 protein, and we use comparative genomic analysis to analyze the evolutionary relationships and to infer evoluti ... | 2004 | 15342797 |
| dietary requirement of tryptophan for growth and survival of the indian major carp, cirrhinus mrigala (hamilton-buchanan) fry. | the experimental diets (40% protein) contained graded levels of tryptophan (0.60, 0.75, 1.00, 1.25, 1.50, 1.75%) of dietary protein. each test diet was fed to triplicate groups of mrigal fry twice daily at 10% of the total biomass for 8 weeks. the dietary tryptophan requirement was estimated by plotting weight gain against dietary levels of tryptophan using two separate regression equations, the point of intersection of two equations was taken as optimum level, which occurred at 0.48% of the die ... | 2003 | 15332510 |
| designing of novel carbonic anhydrase inhibitors and activators. | carbonic anhydrases (cas, ec 4.2.1.1) are wide spread enzymes, present in mammals in at least 14 different isoforms: some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), while others are membrane-bound (ca iv, ca ix, ca xii and ca xiv); ca v is mitochondrial, and ca vi is secreted in the saliva. three acatalytic forms are also known (carp viii, carp x and carp xi). several important physiological and physio-pathological functions are played by many ca isozymes, which are strongly ... | 2004 | 15328829 |
| stabilisation of scoliosis in two koi (cyprinus carpio). | two koi (cyprinus carpio) from the same pond developed similar lesions of scoliosis. radiographic examinations showed that their spines had become malaligned as a result of vertebral compression fractures involving t14 to t16. the vertebrae in both fish were stabilised with screws, k-wire and polymethylmethacrylate. they both appeared to improve after surgery, but they began to decline and died within three months. a postmortem examination revealed multi-organ inflammation that was not associate ... | 2004 | 15328741 |
| designing of novel carbonic anhydrase inhibitors and activators. | carbonic anhydrases (cas, ec 4.2.1.1) are wide spread enzymes, present in mammals in at least 14 different isoforms: some of these isozymes are cytosolic (ca i, ca ii, ca iii, ca vii), while others are membrane-bound (ca iv, ca ix, ca xii and ca xiv); ca v is mitochondrial, and ca vi is secreted in the saliva. three acatalytic forms are also known (carp viii, carp x and carp xi). several important physiological and physio-pathological functions are played by many ca isozymes, which are strongly ... | 2004 | 15320807 |
| visible light affects chemiluminescence of carp (cyprinus carpio) blood leukocytes. | visible light irradiations at doses of 5 and 12 j/cm(2) were applied to carp buffy coat leukocytes. | 2004 | 15315734 |
| nitrite toxicity in indian major carps: sublethal effect on selected enzymes in fingerlings of catla catla, labeo rohita and cirrhinus mrigala. | the effects of 96-h sublethal exposure of nitrite (1, 2, 4, 8 and 10.4 mg l(-1)) on selected enzymatic activities in serum and tissues of fingerlings of catla (catla catla), rohu (labeo rohita) and mrigal (cirrhinus mrigala) were studied for the first time in these species. all three species responded almost identically to nitrite exposure. with increasing nitrite concentration, reduction in activities was observed in acetylcholinesterase (ache) in brain and liver; alkaline phosphatase (alp) in ... | 2004 | 15313441 |
| in situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems. | effluents are a main source of direct and often continuous input of pollutants into aquatic ecosystems with long-term implications on ecosystem functioning. therefore, the study of the effects of effluent exposure on organisms, populations or communities within the framework of impact assessment has a high ecological relevance. the aim of this study was to assess the toxicological impact of two effluents, one household wastewater treatment effluent (effluent 1) and one industrial effluent (efflu ... | 2004 | 15312937 |
| cloning and characterisation of natural resistance associated macrophage protein (nramp) cdna from red sea bream (pagrus major). | nramp (natural resistance associated macrophage protein) controls aspects of innate resistance to intracellular parasites. its function is to enhance the ability of macrophages to kill pathogens. however, little is known about the structure and function of nramp in lower vertebrates such as teleosts. we have recently isolated a cdna encoding nramp from spleen of red sea bream (pagrus major). the full-length cdna of the nramp is 4709 bp in length, including 197 bp 5'-terminal untranslated region ... | 2004 | 15312657 |
| effect of naproxen on tail fin regeneration in teleost. | in the present investigation we studied the effect of naproxen, a non-steroidal anti-inflammatory drug used for the treatment of rheumatic disease, on the synthesis of the lepidotrichial matrix of the tail fin of the teleost fish cyprinus carpio (carp) during the regeneration process. the lepidotrichial synthesis was observed by standard and polarized light microscopy and by transmission electron microscopy. in general, naproxen at the dose used in the present study did not affect the organizati ... | 2004 | 15311675 |
| trends in two year risk of repeat revascularisation or death from cardiovascular disease after coronary artery bypass grafting or percutaneous coronary intervention in western australia, 1980-2001. | to investigate whether, over the 21 year period 1980-2001, there had been a reduction in the risk of repeat revascularisation or death from cardiovascular disease in the cohort of all patients who were treated by coronary revascularisation in western australia. | 2004 | 15310696 |
| trends in coronary artery revascularisation procedures in western australia, 1980-2001. | to describe trends in the use of coronary artery revascularisation procedures (carps) and to determine whether or when carp rates will stabilise. | 2004 | 15310694 |
| pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus. | a lethal disease of koi and common carp (species cyprinus carpio) has afflicted many fish farms worldwide since 1998, causing severe financial losses. morbidity and mortality are restricted to common carp and koi and appear in spring and autumn, when water temperatures are 18 to 28 degrees c. we have isolated the virus causing the disease from sick fish, propagated it in koi fin cell culture, and shown that virus from a single clone causes lethal disease in carp and koi upon infection. intraperi ... | 2004 | 15308746 |
| hypoxic survival strategies in two fishes: extreme anoxia tolerance in the north european crucian carp and natural hypoxic preconditioning in a coral-reef shark. | especially in aquatic habitats, hypoxia can be an important evolutionary driving force resulting in both convergent and divergent physiological strategies for hypoxic survival. examining adaptations to anoxic/hypoxic survival in hypoxia-tolerant animals may offer fresh ideas for the treatment of hypoxia-related diseases. here, we summarise our present knowledge of two fishes that have evolved to survive hypoxia under very different circumstances. the crucian carp (carassius carassius) is of part ... | 2004 | 15299034 |
| effects of four chlorobenzenes on serum sex steroids and hepatic microsome enzyme activities in crucian carp, carassius auratus. | four chlorobenzenes (chlorobenzene, 1,3-dichlorobenzene, 1,4-dichlorobenzene, p-chloro-methylbenzene) were administrated to the crucian carps (carassius auratus) by peritoneal injections in the laboratory for 30 days. serum testosterone and 17 beta-estradiol concentrations were detected using radioimmunology assay (ria), and the activities of two hepatic microsome enzymes, glutathione s-transferase (gst) and udp-glucuronosyltransferase (udpgt), were measured using the modified methods as describ ... | 2004 | 15294436 |
| vertebrate brains at the pilot light. | while the brains of most vertebrates are unable to tolerate more than a few minutes of anoxia, some freshwater turtles (trachemys and chrysemys), crucian carp (carassius carassius) and frogs (rana pipens and rana temporaria) can survive anoxia for hours to months. obviously, anoxia tolerance has evolved separately several times and this is also reflected in the divergent strategies these animals utilize to survive without oxygen. the turtles and crucian carp defend their brain atp levels and avo ... | 2004 | 15288600 |
| immunohistochemical distribution of neuropeptide y in the mesencephalon and rhombencephalon of carp, cyprinus carpio l. (cyprinidae: teleostei). | the localization of neuropeptide y (npy)-immunoreactive elements was investigated in the mesencephalon and rhombencephalon of carp, cyprinus carpio, by using antisera raised against porcine npy and the immunoperoxidase technique. concurrently, to identify the distribution of npy-immunoreactivity, we developed an atlas of the studied areas based on nissl-stained sections. the npy-immunoreactive (npy-ir) elements were located in many zones of the mesencephalon and rhombencephalon. in the mesenceph ... | 2004 | 15275652 |
| acute toxicity of lake water to fishes. | the present study investigates the acute toxicity of hussainsagar lake water to fishes. in this context, experiments were conducted on three species of fishes (cyprinus carpio, tilapia mozambica and lebistus reticularis), using 5, 10 and 20% volume of jeedimetla nallah wastewater and were observed for the signs of survival within the specified period of time. the tlm value for 96 hours test period was found to be 17%. similarly toxicity studies were carried out on hussainsagar lake water with di ... | 2003 | 15270345 |
| characterization of a new c-type lectin from common carp cyprinus carpio. | a new c-type lectin with putative mannose specificity was identified from the expressed sequence tag (est) analysis of cdna library from common carp head kidney (hk), stimulated with concanavalin a and lipopolysaccharide (lps) during a routine est analysis. the full sequence of 627 bp was identified by 5'-rapid amplification of cdna ends. the gene is composed of 146 amino acid residues, including an 18-residue signal peptide for secretion and a single carbohydrate-recognition domain of approxima ... | 2004 | 15261461 |
| a 1-mb critical region in six patients with 9q34.3 terminal deletion syndrome. | patients with 9q34.3 terminal deletion usually show a clinically recognizable phenotype characterized by specific facial features (microcephaly, flat face, arched eyebrows, hypertelorism, short nose, anteverted nostrils, carp mouth and protruding tongue) in combination with severe mental retardation, hypotonia, and other anomalies. we analyzed six unrelated patients with a various 9q34.3 terminal deletion. while having different-sized 9q34.3 deletions, all of these patients shared several distin ... | 2004 | 15258833 |
| identification of a novel c1q family member in color crucian carp (carassius auratus) ovary. | potential roles of c1q/tumor necrosis factor (tnf) superfamily proteins have been observed in vertebrate oogenesis and oocyte maturation, but no ovary-specific member has been identified so far. in this study, we have cloned and identified a novel member of c1q family with a c1q domain in the c-terminal from fully grown oocyte cdna library of color crucian carp and demonstrated that the gene might be specifically expressed in ovary and therefore designated as carassius auratus ovary-specific c1q ... | 2004 | 15253877 |
| seasonality of dihydropyridine receptor binding in the heart of an anoxia-tolerant vertebrate, the crucian carp (carassius carassius l.). | prolonged anoxia tolerance of facultative anaerobes is based on metabolic depression and thus on controlled reduction of energy-utilizing processes. one proposed survival mechanism is the closing of ion channels to decrease energetic cost of ion pumping (hochachka pw. science 231: 234-241, 1986). to test this hypothesis, the involvement of l-type ca2+ channels in seasonal anoxia tolerance of the vertebrate heart was examined by determining the number of [methyl-3h]pn200-110 (a ligand of l-type c ... | 2004 | 15242827 |
| molecular cloning and expression of preproadrenomedullin gene from common carp cyprinus carpio l. | a preproadrenomedullin (preproam) gene was isolated from common carp cyprinus carpio l. by pcr mediated homology cloning. the isolated gene was composed of 516bp, which translated to a protein having 171 amino acid residues. the signal peptide was composed of 23 amino acid residues and predicted adrenomedullin (am) was conserved in this sequence and made up of 49 amino acid residues. am gene from carp shared identities of 64.0, 44.2, and 46.0% to takifugu (am-1), human, and rat am orthologues, r ... | 2004 | 15242754 |
| decontamination effect of electrolysed nacl solutions on carp. | to evaluate the efficacy of electrolysed nacl solutions (ew) for disinfecting bacterial isolates from carp, and the potential application of ew to reducing the bacterial load in whole carp and carp fillets. | 2004 | 15242456 |
| temperature acclimation modifies na+ current in fish cardiac myocytes. | the present study was designed to test the hypothesis that temperature acclimation modifies sarcolemmal na+ current (ina) of the fish cardiac myocytes differently depending on the animal's lifestyle in the cold. two eurythermal fish species with different physiological strategies for surviving in the cold, a cold-dormant crucian carp (carassius carassius l.) and a cold-active rainbow trout (oncorhynchus mykiss), were used in acclimation experiments. the ina of carp and trout were also compared w ... | 2004 | 15235011 |
| apolipoproteins a-i and a-ii are potentially important effectors of innate immunity in the teleost fish cyprinus carpio. | we have previously shown that high density lipoprotein is the most abundant protein in the carp plasma and displays bactericidal activity in vitro. therefore the aim of this study was to analyze the contribution of its principal apolipoproteins, apoa-i and apoa-ii, in defense. both apolipoproteins were isolated by a two step procedure involving affinity and gel filtration chromatography and were shown to display bactericidal and/or bacteriostatic activity in the micromolar range against gram-pos ... | 2004 | 15233794 |
| modulative influence of lysozyme dimer on defence mechanisms in the carp (cyprinus carpio) and european sheatfish (silurus glanis) after suppression induced by herbicide roundup. | immunomodulation is a commonly used method of prophylaxis in humans and animals. lysozyme dimer (klp-602) was used at a dose of 50 ug/kg b.w. in order to correct the immunosuppression caused by the action of herbicide glyphosate (roundup- monsanto), which was used in a single bath for 10 minutes in a concentration of 100 mg/l of water. the investigations were carried out on 2 species of fish: the carp (cyprinus carpio l.) and european catfish (silurus glanis l.). herbicide glyphosate caused a de ... | 2004 | 15230544 |
| modulation in hepatic and head kidney parameters of carp (cyprinus carpio l.) induced by copper and chitosan. | copper is used in treatment mixtures to control fungal diseases in vineyards plants. high concentrations of copper are inducing antioxidant stress in some aquatic ecosystems, and potential bioaccumulation in aquatic organisms has prompted the demand for alternative use of low toxic molecules in culture treatments. chitosan is a biomolecule with antifungal and heavy metal ion chelating properties that may be used as a biopesticide. in this study, we investigate the potential toxicity of chitosan ... | 2004 | 15228950 |
| localization, expression and control of adrenocorticotropic hormone in the nucleus preopticus and pituitary gland of common carp (cyprinus carpio l.). | adrenocorticotropic hormone (acth) takes a central role in the hypothalamo-pituitary-interrenal axis (hpi axis), which is activated during stress. acth is produced by the corticotrope cells of the pituitary pars distalis (pd) and is under control of factors from the nucleus preopticus (npo). the distribution of acth in the hypothalamo-pituitary system in common carp (cyprinus carpio l.) was assessed by immunohistochemistry. acth and beta-endorphin immunoreactivity was observed in the acth cells ... | 2004 | 15225128 |
| msap, the meichroacidin homolog of carp (cyprinus carpio), differs from the rodent counterpart in germline expression and involves flagellar differentiation. | to gain access to the molecular mechanisms of spermatogenesis, the genes from a subtractive screen of the carp testis cdna library were investigated. in this study, a male-specific homolog of the meichroacidin gene, called msap (morn motif-containing sperm-specific axonemal protein), was isolated and further characterized. database search and zoo-western blot analyses revealed that msap homologs might be widespread in a variety of phyla but divergent in their c-terminal length and sequences. car ... | 2004 | 15215198 |
| specific cytotoxic activity generated by mixed leucocyte culture in ginbuna crucian carp. | 2004 | 15212738 | |
| in vitro effects of steroid hormones on igm-secreting cells and igm secretion in common carp (cyprinus carpio). | the effects of steroid hormones on in vitro igm-secreting cells (igmsc) and igm secretion by lymphocytes of the lymphoid organs in common carp, cyprinus carpio were examined by elispot and elisa assay, respectively. cells isolated from peripheral blood (pb), spleen and head kidney were cultured for 12, 24 and 48 h either in the absence or in the presence of steroid hormones, i.e. cortisol, testosterone, 11-ketotestosterone (11-kt), and estradiol-17beta (e(2)) at doses of 1, 10 and 100 ng/ml. cor ... | 2004 | 15212735 |
| molecular phylogeny of congeneric monogenean parasites (dactylogyrus): a case of intrahost speciation. | dactylogyrus species (dactylogyridae: monogenea) are a group of monogenean gill parasites that are highly specific to freshwater fish of the family cyprinidae. dactylogyrus species were sampled from 19 cyprinids and one percid collected in europe. using partial 18s rdna and its1 sequences, a phylogeny of 51 dactylogyrus species was reconstructed to investigate the patterns of parasite speciation and diversification. three main dactylogyrus lineages were recognized from all phylogenetic trees, th ... | 2004 | 15212381 |
| hydrodynamics of burst swimming fish larvae; a conceptual model approach. | burst swimming of fish larvae is analysed from a hydrodynamic point of view. a picture of the expected flow pattern is presented based on information in literature on unsteady-flow patterns around obstacles in the intermediate reynolds number region. it is shown that the acceleration stage of burst swimming under restricted conditions can be treated as a frictionless impulsive motion. the stream pattern resulting from this motion is presented and the efficiency of locomotion during the accelerat ... | 2004 | 15207478 |
| growth and development of catla (catla catla) fed with different levels of diet containing spirogyra sp. | the effect of spirogyra sp. incorporated into diet formulations on the growth and body composition of indian major carp, catla (catla catla) was investigated in a 45 days feeding trial. spirogyra dry powder was mixed with different feed ingredients in different amounts (0%, 10%, 25%, 37% and 40% of the total feed). carps fed with spirogyra demonstrated higher feed conversion ratio. the study also revealed a direct relationship between the amount of spirogyra in the diet, and muscle protein and f ... | 2004 | 15207298 |
| molecular cloning of proopiomelanocortin cdna and multi-tissue mrna expression in channel catfish. | channel catfish (ictalurus punctatus) proopiomelanocortin (pomc) cdna was cloned to investigate its structure, evolution, and expression in different tissues. pomc is an important gene in the hypothalamus-pituitary-adrenal axis, the main mediator of the stress response. pomc gene was isolated from a pituitary cdna library and nucleotide sequence was determined. pomc cdna is composed of 1164 nucleotides with a 639 nucleotide open reading frame encoding a protein of 212 amino acids. catfish pomc p ... | 2004 | 15201069 |
| effect of cadmium on blood plasma biochemistry in carp (cyprinus carpio l.). | 2004 | 15199987 | |
| effect of cadmium on hematological indices of common carp (cyprinus carpio l.). | 2004 | 15199986 | |
| sequences of transgene insertion sites in transgenic f4 common carp. | 2004 | 15198197 | |
| hematologic reference intervals for koi (cyprinus carpio), including blood cell morphology, cytochemistry, and ultrastructure. | hematologic data are used routinely in the health care of humans and domestic mammals. similar data for fish are largely fragmentary or have not been collected. | 2004 | 15195266 |
| metabolic responses to low temperature in fish muscle. | for most fish, body temperature is very close to that of the habitat. the diversity of thermal habitats exploited by fish as well as their capacity to adapt to thermal change makes them excellent organisms in which to examine the evolutionary and phenotypic responses to temperature. an extensive literature links cold temperatures with enhanced oxidative capacities in fish tissues, particularly skeletal muscle. closer examination of inter-species comparisons (i.e. the evolutionary perspective) in ... | 2004 | 15191230 |
| epithelio-reticular cell thymoma in carp, cyprinus carpio l: an ultrastructural study. | 2004 | 15189377 | |
| response of cyprinus carpio (linn) to sublethal concentration of cypermethrin: alterations in protein metabolic profiles. | freshwater fish, cyprinus carpio were exposed to sublethal concentration (1.2 microg l(-1)) of cypermethrin for 6, 12, 24 and 48 h to analyze various parameters of protein metabolism in functionally different tissues. total, structural and soluble proteins showed decrement; where as free amino acids and the activities of protease, aspartate aminotransferase and alanine aminotransferase significantly increased in cypermethrin exposed fish. interestingly, ammonia content decreased but urea and glu ... | 2004 | 15183996 |
| purification and functional assessment of c3a, c4a and c5a of the common carp (cyprinus carpio) complement. | promotion of inflammatory response is an important role of the complement system, but this kind of function is poorly documented for the lower vertebrates. here we report chemotactic activity of purified anaphylactic fragments derived from the complement components c3, c4 and c5 of the common carp. the purified anaphylatoxins are two c5a-desarg peptides derived from the c5-i isotype, an intact form and a desarg form of c4a from c4-2 isotype, and an intact form and a desarg form of c3a from c3-h1 ... | 2004 | 15183031 |
| cell culture of clonal ginbuna crucian carp hematopoietic cells: differentiation of cultured cells into erythrocytes in vivo. | we cultivated kidney hematopoietic cells from clonal triploid ginbuna crucian carp, carassius auratus langsdorfii. proliferating cells from hematopoietic cell cultures were harvested and injected into tetraploid hybrids (clonal triploid ginbuna and goldfish hybrid) which possess three sets of chromosomes from a triploid clone and a haploid set of chromosomes from goldfish (carassius auratus). after injection of cultured triploid cells (donor cells), blood samples were obtained from tetraploid hy ... | 2004 | 15183027 |
| cloning of a chub metallothionein cdna and development of competitive rt-pcr of chub metallothionein mrna as a potential biomarker of heavy metal exposure. | metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. we sequenced chub (leuciscus cephalus) metallothionein cdna which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. we then used the extended primer method to develop an accurate quantitative competitive rt-pcr assay for metallothionein mrna. rt-pcr was used to measure metallothionein mrna in feral chub from a ra ... | 2015 | 15178096 |
| an in vitro procedure for evaluation of early stage oxidative stress in an established fish cell line applied to investigation of phah and pesticide toxicity. | oxidative stress by increased production of reactive oxygen species such as superoxide has been implicated in the toxicity of pcb's and non-target toxicity of many pesticides. we report the development of a microplate-based method for determination of early stage oxidative stress using an established cell line (epc) from a skin tumour of carp cyprinus carpio l. and 2',7'-dichlorodihydrofluorescein diacetate (h(2)-dcfda) as a fluorescent probe for detection of reactive oxygen species (ros) format ... | 2014 | 15178091 |
| identification of the multidrug resistance-associated protein (mrp) related gene in red mullet (mullus barbatus). | multixenobiotic resistance mechanism (mxr) in aquatic organisms is mediated by the activity of the p-glycoprotein (pgp) transporter that binds and actively effluxes different chemicals out of cell. in addition to the pgp, several other, non-pgp transport proteins have been recently identified in different human and animal tissues. given their characteristics and tissue distribution we hypothesized that members of the so-called multidrug resistance-associated protein (mrp) family may be expressed ... | 2004 | 15178032 |
| humoral immune factors modulated by copper and chitosan in healthy or parasitised carp (cyprinus carpio l.) by ptychobothrium sp. (cestoda). | as an environmental protection point of view, the potential toxicity of chitosan on aquatic animal health, alone or associated with copper must be investigated. fish possess defence mechanisms to counteract the impact of toxics. the non-cellular and non-specific immune defences (total immunoglobulin, ceruloplasmin, lysozyme and potential killing activity of phagocytic cells) can be modulated by the potential environmental pollutants but also by natural stimulants such as bacteria, viruses or par ... | 2004 | 15177950 |
| endocrine disruption and altered gonadal development in white perch (morone americana) from the lower great lakes region. | high prevalences of gonadal intersex have been observed in wild fish populations in areas affected by domestic and industrial effluents. for this study, fish were collected in 1998 from the cootes paradise region of hamilton harbour in western lake ontario, canada, to determine whether gonadal abnormalities, including intersex, were present in young of the year (yoy) fish. no gonadal abnormalities were observed in goldfish (carassius auratus), common carp (cyprinus carpio), gizzard shad (dorosom ... | 2004 | 15175179 |
| levels of synthetic musk compounds in municipal wastewater for potential estimation of biota exposure in receiving waters. | we analyzed water samples from the confluence of three municipal sewage treatment effluent streams, surface water, and whole carp (cyprinus carpio) for synthetic musks for a period of 7-12 months. the lipid content of each fish was determined and compared with the concentration of musks in the whole fish tissue. enhanced methods were used for water sampling and musk extraction. the data presented here provide insight as to the relationship between concentrations of synthetic musks in the municip ... | 2004 | 15173905 |
| vitellogenin in rare minnow (gobiocypris rarus): identification and induction by waterborne diethylstilbestrol. | rare minnow (gobiocypris rarus) is a tiny chinese carp that has a short life cycle and is easily cultured in the laboratory. in this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (des) at 0.05, 0.5 and 5 microg/l in laboratory aquaria. after exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (vtg) was measured in whole body homogenates. native and sds electrophoresis followed by western blotting were performed for vtg identification, and ... | 2004 | 15171953 |
| structural characterisation of a cyprinid (cyprinus carpio l.) crh, crh-bp and crh-r1, and the role of these proteins in the acute stress response. | we elucidated the structure of the principle factors regulating the initiation of the acute stress response in common carp: corticotrophin-releasing hormone (crh), crh-receptor 1 (crh-r1) and crh-binding protein (crh-bp). phylogenetic analyses reveal that these proteins are evolutionarily well conserved in vertebrates. crh and crh-bp expression are not co-localised in the same hypothalamic perikarya. on the contrary, crh-bp expression is limited to the perimeter of the nucleus preopticus (npo), ... | 2004 | 15171705 |
| tracking of the hormonally induced maturation of female eels by computed tomography. | female european eels were kept in artificial seawater for a trial period of 14 weeks. three fish were injected intra-abdominally with carp pituitary suspension (twice a week) and human chorionic gonadotropin (every 2nd week), with the aim to induce artificial maturation. three further fish were not treated (control). fish were not fed during the trial. the treated fish were scanned by computed tomography (ct) every second week (the controls only at the start and at the end of the trial) to follo ... | 2004 | 15168755 |
| the fish fauna in tropical rivers: the case of the sorocaba river basin, são paulo, brazil. | a survey was carried out on the fish species in the sorocaba river basin, the main tributary of the left margin of the tietê river, located in the state of são paulo, brazil. the species were collected with gill nets. after identification of the specimens, their relative abundance, weight and standard length were determined. up to the present moment there are not any studies that focus this subject in this hydrographic basin. fifty-three species, distributed in eighteen families and six orders w ... | 2003 | 15162785 |
| effects of natural and synthetic estrogens and various environmental contaminants on vitellogenesis in fish primary hepatocytes: comparison of bream (abramis brama) and carp (cyprinus carpio). | interaction of environmental estrogens with the estrogen receptor (er) has been shown in various fish species. our objective was to compare the sensitivity of bream (abramis brama) to (xeno-)estrogens with that of the carp (cyprinus carpio), by measuring the effects of 17beta-estradiol (e2), estrone (e1), ethynylestradiol (ee2), bisphenol a (bpa), nonylphenol (np), methoxychlor (mxcl), and halogenated aromatic hydrocarbons (hahs) such as polychlorinated biphenyls (pcb126, pcb118), 2,3,7,8-tetrac ... | 2004 | 15159526 |