Publications

comparability between insecticide resistance bioassays for mosquito vectors: time to review current methodology?	insecticides play an integral role in the control of mosquito-borne diseases. with resistance to insecticides on the rise, surveillance of the target population for optimal choice of insecticides is a necessity. the centers for disease control and prevention (cdc) bottle assay and the world health organization (who) susceptibility test are the most frequently used methods in insecticide resistance monitoring. however, the two bioassays differ in terms of insecticide delivery and how insecticide ...	2015	26148484
[microgeographic and temporal genetic changes of aedes aegypti from medellín, colombia].	aedes aegypti populations may experience changes in abundance and genetic diversity in addition to changes in their evolutionary capability to respond to vector control. the knowledge on the changes in genetic variation on a spatio-temporal scale improves the epidemiological understanding of dengue and supports the appropriate and timely design of vector control strategies.	2015	26148034
[insecticide susceptibility evaluation in aedes aegypti populations of caldas, colombia, in 2007 and 2011].	continuous use of insecticides for the control of dengue transmission may lead to decreased susceptibility levels in mosquito vector populations. timely monitoring is necessary to ensure detection of any potential resistance problems.	2015	26148033
a stochastic model to study rift valley fever persistence with different seasonal patterns of vector abundance: new insights on the endemicity in the tropical island of mayotte.	rift valley fever (rvf) is a zoonotic vector-borne disease causing abortion storms in cattle and human epidemics in africa. our aim was to evaluate rvf persistence in a seasonal and isolated population and to apply it to mayotte island (indian ocean), where the virus was still silently circulating four years after its last known introduction in 2007. we proposed a stochastic model to estimate rvf persistence over several years and under four seasonal patterns of vector abundance. firstly, the mo ...	2015	26147799
cockroach, tick, storage mite and other arthropod allergies: where do we stand with molecular allergy diagnostics?: part 15 of the series molecular allergology.	arthropods form a broad phylum within the animal kingdom, comprising widely varying members such as insects, arachnids, crabs and centipedes. in addition to common allergies to house dust mites or hymenoptera venom, there are also rarer allergies that can be attributed to three major sources of allergens: cockroaches, ticks and storage mites. other less known allergen sources include spiders, mosquitos, horseflies, red chironomid larvae, silverfish and ladybugs, as well as a variety of storage p ...	2014	26146603
a comparative analysis of reproductive biology of insect vectors of human disease.	studying the reproductive strategies of insect species that transmit diseases to humans can identify new exploitable targets for the development of vector control methods. here we describe shared characteristics and individual features of the reproductive biology of three major disease vectors: anopheles gambiae, aedes aegypti and glossina morsitans. current studies are identifying i) species-specific molecular cascades that determine female monandrous behavior, ii) core aspects of egg developme ...	0	26140265
chikungunya outbreak in garo hills, meghalaya: an epidemiological perspective.	chikungunya (chik) fever is a mosquito-borne disease caused by chikungunya virus (chikv). chikungunya infection was first reported from india in 1963 from kolkata. we report the serological and molecular evidence of an outbreak of chikungunya in northeast india that occurred in tura, a hilly and forested terrain in garo hills district of meghalaya.	0	26139776
emergence of dengue in tribal villages of mandla district, madhya pradesh, india.	dengue (den) is a rapidly spreading arboviral disease transmitted by aedes mosquitoes. although it is endemic in india, dengue virus (denv) infection has not been reported from tribal areas of madhya pradesh. investigations were conducted to establish the aetiology of sudden upsurge of cases with febrile illness in june 2013 from tribal villages of mandla district of madhya pradesh, india.	0	26139775
insecticidal potency of aspergillus terreus against larvae and pupae of three mosquito species anopheles stephensi, culex quinquefasciatus, and aedes aegypti.	microbial control agents offer alternatives to chemical pest control, as they can be more selective than chemical insecticides. the present study evaluates the mosquito larvicidal and pupicidal potential of fungus mycelia using ethyl acetate and methanol solvent extracts produced by aspergillus terreus against anopheles stephensi, culex quinquefasciatus, and aedes aegypti. the a. terreus mycelia were extracted after 15 days from sabouraud dextrose broth medium. the ethyl acetate extracts showed ...	2015	26139412
a fungicide-responsive kinase as a tool for synthetic cell fate regulation.	engineered biological systems that precisely execute defined tasks have major potential for medicine and biotechnology. for instance, gene- or cell-based therapies targeting pathogenic cells may replace time- and resource-intensive drug development. engineering signal transduction systems is a promising, yet presently underexplored approach. here, we exploit a fungicide-responsive heterologous histidine kinase for pathway engineering and synthetic cell fate regulation in the budding yeast saccha ...	2015	26138483
movement of regulatory rna between animal cells.	recent studies suggest that rna can move from one cell to another and regulate genes through specific base-pairing. mechanisms that modify or select rna for secretion from a cell are unclear. secreted rna can be stable enough to be detected in the extracellular environment and can enter the cytosol of distant cells to regulate genes. mechanisms that import rna into the cytosol of an animal cell can enable uptake of rna from many sources including other organisms. this role of rna is akin to that ...	2015	26138457
cloning, expression analysis, and rna interference study of a horma domain containing autophagy-related gene 13 (atg13) from the coleopteran beetle, tenebrio molitor.	autophagy is a process that is necessary during starvation, as it replenishes metabolic precursors by eliminating damaged organelles. autophagy is mediated by more than 35 autophagy-related (atg) proteins that participate in the nucleation, elongation, and curving of the autophagosome membrane. in a pursuit to address the role of autophagy during development and immune resistance of the mealworm beetle, tenebrio molitor, we screened atg gene sequences from the whole-larva transcriptome database. ...	2015	26136688
molecular cloning and mrna expression of heat shock protein genes and their response to cadmium stress in the grasshopper oxya chinensis.	heat shock proteins (hsps) are highly conserved molecular chaperones that are synthesized in response to stress. in this study, we cloned the full-length sequences of the grp78 (glucose-regulated protein 78), hsp70, hsp90, and hsp40 genes from the chinese rice grasshopper oxya chinensis. the full-length cdna sequences of ocgrp78, ochsp70, ochsp90, and ochsp40 contain open reading frames of 1947, 1920, 2172, and 1042 bp that encode proteins of 649, 640, 724, and 347 amino acids, respectively. flu ...	2015	26135744
unravelling the evolution of the allatostatin-type a, kiss and galanin peptide-receptor gene families in bilaterians: insights from anopheles mosquitoes.	allatostatin type a receptors (ast-ars) are a group of g-protein coupled receptors activated by members of the fgl-amide (ast-a) peptide family that inhibit food intake and development in arthropods. despite their physiological importance the evolution of the ast-a system is poorly described and relatively few receptors have been isolated and functionally characterised in insects. the present study provides a comprehensive analysis of the origin and comparative evolution of the ast-a system. to ...	2015	26135459
insecticidal potential of ocimum canum plant extracts against anopheles stephensi, aedes aegypti and culex quinquefasciatus larval and adult mosquitoes (diptera: culicidae).	mosquitoes have developed resistance to various synthetic insecticides, making their control increasingly difficult. insecticides of botanical origin may serve as suitable natural control. this study evaluates the toxic potential of ocimum canum (sims) leaf extract and powder against anopheles stephensi (liston), aedes aegypti (lin) and culex quinquefasciatus (say) larval and adult mosquitoes. larval mortality was observed after 24 h recovery period and adult smoke toxicity observed for 40 min d ...	2016	26135241
suppression of a field population of aedes aegypti in brazil by sustained release of transgenic male mosquitoes.	the increasing burden of dengue, and the relative failure of traditional vector control programs highlight the need to develop new control methods. sit using self-limiting genetic technology is one such promising method. a self-limiting strain of aedes aegypti, ox513a, has already reached the stage of field evaluation. sustained releases of ox513a ae. aegypti males led to 80% suppression of a target wild ae. aegypti population in the cayman islands in 2010. here we describe sustained series of f ...	2015	26135160
should symbionts be nice or selfish? antiviral effects of wolbachia are costly but reproductive parasitism is not.	symbionts can have mutualistic effects that increase their host's fitness and/or parasitic effects that reduce it. which of these strategies evolves depends in part on the balance of their costs and benefits to the symbiont. we have examined these questions in wolbachia, a vertically transmitted endosymbiont of insects that can provide protection against viral infection and/or parasitically manipulate its hosts' reproduction. across multiple symbiont strains we find that the parasitic phenotype ...	2015	26132467
involvement of tetraspanin c189 in cell-to-cell spreading of the dengue virus in c6/36 cells.	dengue virus (denv) is naturally transmitted by mosquitoes to humans, infecting cells of both hosts. unlike in mammalian cells, denv usually does not cause extremely deleterious effects on cells of mosquitoes. despite this, clustered progeny virions were found to form infection foci in a high density cell culture. it is thus interesting to know how the virus spreads among cells in tissues such as the midgut within live mosquitoes. this report demonstrates that cell-to-cell spread is one way for ...	2015	26132143
crispr-cas9 genome editing in drosophila.	the crispr-cas9 system has transformed genome engineering of model organisms from possible to practical. crispr-cas9 can be readily programmed to generate sequence-specific double-strand breaks that disrupt targeted loci when repaired by error-prone non-homologous end joining (nhej) or to catalyze precise genome modification through homology-directed repair (hdr). here we describe a streamlined approach for rapid and highly efficient engineering of the drosophila genome via crispr-cas9-mediated ...	2015	26131852
the global distribution of the arbovirus vectors aedes aegypti and ae. albopictus.	dengue and chikungunya are increasing global public health concerns due to their rapid geographical spread and increasing disease burden. knowledge of the contemporary distribution of their shared vectors, aedes aegypti and aedes albopictus remains incomplete and is complicated by an ongoing range expansion fuelled by increased global trade and travel. mapping the global distribution of these vectors and the geographical determinants of their ranges is essential for public health planning. here ...	2015	26126267
an environmentally safe larvicide against aedes aegypti based on in situ gelling nanostructured surfactant systems containing an essential oil.	liquid crystalline precursors, which are in situ gelling nanostructured surfactant systems, can undergo phase transition in aqueous solution and become more structured aggregates, controlling release of larvicides and acting as biotechnology alternatives for dengue control. such systems can contain bioactive substances as citrus sinensis essential oil (cseo) which exhibits biological activity against aedes aegypti (ae. aegypti) larvae.	2015	26125515
current status of invasive mosquito surveillance in the uk.	non-native invasive mosquitoes have for many years made incursions into europe, and are now established in many european countries. the continued european importation of potential vectors and their expansion within europe increases their potential for importation and establishment in the uk. coupled with increasing numbers of returning dengue and chikungunya infected travellers, the potential exists for transmission of vector borne disease in new regions.	2015	26122427
disruption of dengue virus transmission by mosquitoes.	current control efforts for mosquito-borne arboviruses focus on mosquito control involving insecticide applications, which are becoming increasingly ineffective and unsustainable in urban areas. mosquito population replacement is an alternative arbovirus control concept aiming at replacing virus-competent vector populations with laboratory-engineered incompetent vectors. a prerequisite for this strategy is the design of robust anti-pathogen effectors that can ultimately be genetically driven thr ...	0	26120563
different serotypes of dengue virus (denv) imported by polish travellers from dengue endemic areas to poland.	dengue viruses are the most widespread arboviruses (transmitted mainly by aedes aegypti and ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. there are four dengue virus serotypes: denv-1, denv-2, denv-3, and denv-4. subsequent infections increase the risk of developing severe dengue fever.	2015	26119675
susceptibility status of aedes aegypti (l.) (diptera: culicidae) to temephos from three districts of tamil nadu, india.	dengue is the most rapidly expanding arboviral disease in india. aedes aegypti is the primary vector of dengue fever. chemical insecticides have long been used in the vector control programmes along with other control measures. however, continuous use of insecticides targeting ae. aegypti may lead to development of insecticide resistance. though resistance in ae. aegypti has been reported, the mutation in ace-1 gene associated with temephos resistance is not reported in natural populations. the ...	2015	26119549
boric acid ovicidal trap for the management of aedes species.	the use of low concentrations of boric acid as a potential and effective control agent for the eggs and immature stages of aedes aegypti l. and aedes albopictus skuse (diptera: culicidae) is found to be safe and effective as compared to synthetic chemical insecticides. the study aims to determine the ovicidal concentration of boric acid, its effective concentration for oviposition attraction and the larval mortality concentration for ae. aegypti and ae. albopictus.	2015	26119547
detection of dengue virus in individual aedes aegypti mosquitoes in delhi, india.	delhi, the capital city of india, has so far witnessed several outbreaks of dengue fever since 1967 (last one reported in 2013). improved virological and entomological surveillance are the only tools that can help in prevention of dengue as well as in the development of dengue control programmes. the aim of the study was to conduct a prospective field study to detect dengue virus in adult aedes aegypti mosquitoes collected from various localities represented by different socioeconomic groups in ...	2015	26119544
evaluation of a push-pull approach for aedes aegypti (l.) using a novel dispensing system for spatial repellents in the laboratory and in a semi-field environment.	the increase in insecticide resistant mosquito populations necessitates the exploration of novel vector control intervention measures. push-pull strategies for insect control have been successful when used in integrated crop pest management. through the combinatory use of deterring and attracting stimuli, the abundance of insect pests can be changed in a given area. a push-pull strategy might also significantly reduce human-vector contacts and augment existing mosquito control strategies, e.g. t ...	2015	26115365
wolbachia reduces the transmission potential of dengue-infected aedes aegypti.	dengue viruses (denv) are the causative agents of dengue, the world's most prevalent arthropod-borne disease with around 40% of the world's population at risk of infection annually. wolbachia pipientis, an obligate intracellular bacterium, is being developed as a biocontrol strategy against dengue because it limits replication of the virus in the mosquito. the wolbachia strain wmel, which has been introduced into the mosquito vector, aedes aegypti, has been shown to invade and spread to near fix ...	2015	26115104
the dimerization state of the mammalian high mobility group protein at-hook 2 (hmga2).	the mammalian high mobility group protein at-hook 2 (hmga2) is a chromosomal architectural transcription factor involved in cell transformation and oncogenesis. it consists of three positively charged "at-hooks" and a negatively charged c-terminus. sequence analyses, circular dichroism experiments, and gel-filtration studies showed that hmga2, in the native state, does not have a defined secondary or tertiary structure. surprisingly, using combined approaches of 1-ethyl-3-(3-dimethylaminopropyl) ...	2015	26114780
resistance mechanisms of anopheles stephensi (diptera: culicidae) to temephos.	anopheles stephensi is a sub-tropical species and has been considered as one of the most important vector of human malaria throughout the middle east and south asian region including the malarious areas of southern iran. current reports confirmed an. stephensi resistance to temephos in oman and india. however, there is no comprehensive research on mechanisms of temephos resistance in an. stephensi in the literature. this study was designed in order to clarify the enzymatic and molecular mechanis ...	2014	26114145
phyloproteomic analysis of 11780 six-residue-long motifs occurrences.	how is it possible to find good traits for phylogenetic reconstructions? here, we present a new phyloproteomic criterion that is an occurrence of simple motifs which can be imprints of evolution history. we studied the occurrences of 11780 six-residue-long motifs consisting of two randomly located amino acids in 97 eukaryotic and 25 bacterial proteomes. for all eukaryotic proteomes, with the exception of the amoebozoa, stramenopiles, and diplomonadida kingdoms, the number of proteins containing ...	2015	26114101
targeting male mosquito mating behaviour for malaria control.	malaria vector control relies heavily on the use of long-lasting insecticidal nets (llins) and indoor residual spraying (irs). these, together with the combined drug administration efforts to control malaria, have reduced the death toll to less than 700,000 deaths/year. this progress has engendered real excitement but the emergence and spread of insecticide resistance is challenging our ability to sustain and consolidate the substantial gains that have been made. research is required to discover ...	2015	26113015
standard operating procedures for standardized mass rearing of the dengue and chikungunya vectors aedes aegypti and aedes albopictus (diptera: culicidae) - ii - egg storage and hatching.	management of large quantities of eggs will be a crucial aspect of the efficient and sustainable mass production of mosquitoes for programmes with a sterile insect technique component. the efficiency of different hatching media and effectiveness of long term storage methods are presented here.	2015	26112698
potential prepore trimer formation by the bacillus thuringiensis mosquito-specific toxin: molecular insights into a critical prerequisite of membrane-bound monomers.	the insecticidal feature of the three-domain cry δ-endotoxins from bacillus thuringiensis is generally attributed to their capability to form oligomeric pores, causing lysis of target larval midgut cells. however, the molecular description of their oligomerization process has not been clearly defined. here a stable prepore of the 65-kda trypsin-activated cry4ba mosquito-specific toxin was established through membrane-mimetic environments by forming an ∼200-kda octyl-β-d-glucoside micelle-induced ...	2015	26112409
how urbanization affects the epidemiology of emerging infectious diseases.	the world is becoming more urban every day, and the process has been ongoing since the industrial revolution in the 18th century. the united nations now estimates that 3.9 billion people live in urban centres. the rapid influx of residents is however not universal and the developed countries are already urban, but the big rise in urban population in the next 30 years is expected to be in asia and africa. urbanization leads to many challenges for global health and the epidemiology of infectious d ...	2015	26112265
exploring host-pathogen interactions through biological control.		2015	26110272
lucilia cuprina genome unlocks parasitic fly biology to underpin future interventions.	lucilia cuprina is a parasitic fly of major economic importance worldwide. larvae of this fly invade their animal host, feed on tissues and excretions and progressively cause severe skin disease (myiasis). here we report the sequence and annotation of the 458-megabase draft genome of lucilia cuprina. analyses of this genome and the 14,544 predicted protein-encoding genes provide unique insights into the fly's molecular biology, interactions with the host animal and insecticide resistance. these ...	2015	26108605
the impact of selection with diflubenzuron, a chitin synthesis inhibitor, on the fitness of two brazilian aedes aegypti field populations.	several aedes aegypti field populations are resistant to neurotoxic insecticides, mainly organophoshates and pyrethroids, which are extensively used as larvicides and adulticides, respectively. diflubenzuron (dfb), a chitin synthesis inhibitor (csi), was recently approved for use in drinking water, and is presently employed in brazil for ae. aegypti control, against populations resistant to the organophosphate temephos. however, tests of dfb efficacy against field ae. aegypti populations are lac ...	2015	26107715
phylogeography of the coastal mosquito aedes togoi across climatic zones: testing an anthropogenic dispersal hypothesis.	the coastal mosquito aedes togoi occurs more or less continuously from subarctic to subtropic zones along the coasts of the japanese islands and the east asian mainland. it occurs also in tropical southeast asia and the north american pacific coast, and the populations there are thought to have been introduced from japan by ship. to test this hypothesis, the genetic divergence among geographic populations of a. togoi was studied using one mitochondrial and three nuclear gene sequences. we detect ...	2015	26107619
early origin and adaptive evolution of the gw182 protein family, the key component of rna silencing in animals.	the gw182 proteins are a key component of the mirna-dependent post-transcriptional silencing pathway in animals. they function as scaffold proteins to mediate the interaction of argonaute (ago)-containing complexes with cytoplasmic poly(a)-binding proteins (pabp) and pan2-pan3 and ccr4-not deadenylases. the ago-gw182 complexes mediate silencing of the target mrna through induction of translational repression and/or mrna degradation. although the gw182 proteins are a subject of extensive experime ...	0	26106978
the insecticide susceptibility status of aedes aegypti (diptera: culicidae) in farm and nonfarm sites of lagos state, nigeria.	nigeria is one of the malaria-endemic countries. in lagos state, nigeria, various malaria vector control programs including the use of chemical insecticides are currently being implemented. this study was designed to provide information on the susceptibility status of some nontargeted vectors such as aedes aegypti. adult ae. aegypti mosquitoes from two farm sites and a nonfarm site were exposed to world health organization test papers impregnated with deltamethrin (0.05%), permethrin (0.75%), an ...	2015	26106087
the epidemiology of dengue fever in saudi arabia: a systematic review.	dengue fever (df) is the most serious mosquito-borne viral disease worldwide. df is an acute febrile illness caused by aedes aegypti and aedes albopictus, which are endemic in certain cities of saudi arabia, such as jeddah and makkah (mecca). an online literature search was conducted using relevant keywords to retrieve df studies conducted in saudi arabia. forty-five articles were identified initially. after screening for exclusion and retrieving full texts, a total of 10 articles were used for ...	2016	26106040
modified mosquito landing boxes dispensing transfluthrin provide effective protection against anopheles arabiensis mosquitoes under simulated outdoor conditions in a semi-field system.	efforts to control malaria vectors have primarily focused on scaling-up of long-lasting insecticidal nets (llins) and indoor residual spraying. although highly efficient against indoor-biting and indoor-resting vectors, these interventions have lower impact on outdoor-biting mosquitoes. innovative vector control tools are required to prevent outdoor human-mosquito contacts. in this work, the potential of spatial repellents, delivered in an active system that requires minimal user compliance, to ...	2015	26104719
paratransgenesis: a promising new strategy for mosquito vector control.	the three main mosquito genera, anopheles, aedes and culex, transmit respectively malaria, dengue and lymphatic filariasis. current mosquito control strategies have proved unsuccessful, and there still is a substantial number of morbidity and mortality from these diseases. genetic control methods have now arisen as promising alternative strategies, based on two approaches: the replacement of a vector population by disease-refractory mosquitoes and the release of mosquitoes carrying a lethal gene ...	2015	26104575
saliva from nymph and adult females of haemaphysalis longicornis: a proteomic study.	haemaphysalis longicornis is a major vector of theileria spp., anaplasma phagocytophilum, babesia spp. and coxiella burnetti in east asian countries. all life stages of ixodid ticks have a destructive pool-feeding style in which they create a pool-feeding site by lacerating host tissue and secreting a variety of biologically active compounds that allows the tick to evade host responses, enabling the uptake of a blood meal. the identification and functional characterization of tick saliva protein ...	2015	26104117
[vertical transmission of dengue virus in aedes aegypti, peru].		2015	26102125
[lessons learned in the control of aedes aegypti to address dengue and the emergency of chikungunya in iquitos, peru].	dengue has affected iquitos since 1990 causing outbreaks of major impact on public health and for this reason great efforts have been made for its temporal control. currently, with the expansion of the chikungunya virus in the americas and the threat of the emergence of the virus in iquitos, we reflect on lessons learned by way of the activities undertaken in the area of vector control; epidemiological surveillance, diagnosis and clinical management during periods of outbreaks of dengue, in a wa ...	2015	26102121
[dengue in peru: a quarter century after its reemergence].	a health problem each time more frequent and dispersed in tropical and subtropical areas of the world, including peru where it entered in 1990, is dengue. it is produced by the dengue virus with four serotypes and transmitted by aedes aegypti, a vector that coexists with humans and whose presence is favored by deficient sanitary, social and economic conditions. manifestations of severe forms of the disease such as shock and bleeding, are related to the frequent co-circulation of the four serotyp ...	2015	26102118
contributions of temporal segregation, oviposition choice, and non-additive effects of competitors to invasion success of aedes japonicus (diptera: culicidae) in north america.	the mosquito aedes japonicus (diptera: culicidae) has spread rapidly through north america since its introduction in the 1990s. the mechanisms underlying its establishment in container communities occupied by competitors aedes triseriatus and aedes albopictus are unclear. possibilities include (a) temporal separation of a. japonicus from other aedes, (b) oviposition avoidance by a. japonicus of sites containing heterospecific aedes larvae, and (c) non-additive competitive effects in assemblages ...	0	26101466
proteomic changes occurring in the malaria mosquitoes anopheles gambiae and anopheles stephensi during aging.	the age of mosquitoes is a crucial determinant of their ability to transmit pathogens and their resistance to insecticides. we investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes anopheles gambiae and anopheles stephensi as they aged. protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption i ...	2015	26100052
characterization and bioassay for larvicidal activity of anacardium occidentale (cashew) shell waste fractions against dengue vector aedes aegypti.	recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against aedes aegypti larvae. thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of anacardium occidentale shell wastes against the third and fourth instar larvae of a. aegypti and to compare the results with the commercial larvicide product. the shell wastes were e ...	2015	26099240
heterochromatin, histone modifications, and nuclear architecture in disease vectors.	interactions between a pathogen and a vector are plastic and dynamic. such interactions can be more rapidly accommodated by epigenetic changes than by genetic mutations. gene expression can be affected by the proximity to the heterochromatin, by local histone modifications, and by the three-dimensional position within the nucleus. recent studies of disease vectors indicate that gene regulation by these factors can be important for susceptibility to pathogens, reproduction, immunity, development, ...	0	26097808
surveillance of adult aedes mosquitoes in selangor, malaysia.	to determine the effectiveness of using sticky traps and the ns1 dengue antigen kit for the surveillance of aedes mosquitoes for dengue control.	2015	26094839
predation by asian bullfrog tadpoles, hoplobatrachus tigerinus, against the dengue vector, aedes aegypti, in an aquatic environment treated with mosquitocidal nanoparticles.	aedes aegypti is a primary vector of dengue and chikungunya. the use of synthetic insecticides to control aedes populations often leads to high operational costs and adverse non-target effects. botanical extracts have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles, but their impact against predators of mosquito larvae has not been well studied. we propose a single-step method for the biosynthesis of silver nanoparticles (agnp) using the extract of artemisia vulgar ...	2015	26091763
phytochemical profile and larvicidal properties of seed essential oil from nigella sativa l. (ranunculaceae), against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).	the present study deals with investigation of larvicidal activity and their chemical constituents of the essential oil from the seeds of nigella sativa l. (ranunculaceae). totally, 18 chemical compounds were identified by gc and gc-ms analysis. thymol (19.13 %) and α-phellandrene (14.9 %) were identified as major chemical components followed by camphor (12.14 %), borneol (11.31 %), and carvacrol (8.65 %). the larval mortality was observed after 12 and 24 h of exposure period. the results reveale ...	2015	26091760
proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein.	numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ...	2015	26091699
the role of dmcatd, a cathepsin d-like peptidase, and acid phosphatase in the process of follicular atresia in dipetalogaster maxima (hemiptera: reduviidae), a vector of chagas' disease.	in this work, we have investigated the involvement of dmcatd, a cathepsin d-like peptidase, and acid phosphatase in the process of follicular atresia of dipetalogaster maxima, a hematophagous insect vector of chagas' disease. for the studies, fat bodies, ovaries and hemolymph were sampled from anautogenous females at representative days of the reproductive cycle: pre-vitellogenesis, vitellogenesis as well as early and late atresia. real time pcr (qpcr) and western blot assays showed that dmcatd ...	2015	26091289
knowledge and beliefs about dengue transmission and their relationship with prevention practices in hermosillo, sonora.	dengue is an emerging threat in the u.s.-mexico border region. transmission has regularly occurred in sonora, mx since 1982 but it was not until 2014 that cities directly on the arizona-sonora border had local transmission. one of the closest urban areas to have regular seasonal transmission is hermosillo, sn, mx. developing a better understanding of the knowledge and perceptions of dengue in close geographic proximity to the border can identify areas to target for prevention and control measure ...	2015	26090357
analysing the oviposition behaviour of malaria mosquitoes: design considerations for improving two-choice egg count experiments.	choice egg-count bioassays are a popular tool for analysing oviposition substrate preferences of gravid mosquitoes. this study aimed at improving the design of two-choice experiments for measuring oviposition substrates preferences of the malaria vector anopheles gambiae senso lato, a mosquito that lays single eggs.	2015	26088669
thiosemicarbazones as aedes aegypti larvicidal.	a set of aryl- and phenoxymethyl-(thio)semicarbazones were synthetized, characterized and biologically evaluated against the larvae of aedes aegypti (a. aegypti), the vector responsible for diseases like dengue and yellow fever. (q)sar studies were useful for predicting the activities of the compounds not included to create the qsar model as well as to predict the features of a new compound with improved activity. docking studies corroborated experimental evidence of aescp-2 as a potential targe ...	2015	26087027
climate change and spatiotemporal distributions of vector-borne diseases in nepal--a systematic synthesis of literature.	despite its largely mountainous terrain for which this himalayan country is a popular tourist destination, nepal is now endemic for five major vector-borne diseases (vbds), namely malaria, lymphatic filariasis, japanese encephalitis, visceral leishmaniasis and dengue fever. there is increasing evidence about the impacts of climate change on vbds especially in tropical highlands and temperate regions. our aim is to explore whether the observed spatiotemporal distributions of vbds in nepal can be ...	2015	26086887
the potential use of wolbachia-based mosquito biocontrol strategies for japanese encephalitis.	japanese encephalitis virus (jev) is a zoonotic pathogen transmitted by the infectious bite of culex mosquitoes. the virus causes the development of the disease japanese encephalitis (je) in a small proportion of those infected, predominantly affecting children in eastern and southern asia. annual je incidence estimates range from 50,000-175,000, with 25%-30% of cases resulting in mortality. it is estimated that 3 billion people live in countries in which jev is endemic. the virus exists in an e ...	2015	26086337
a sodium channel variant in aedes aegypti as a candidate pathogen sensor for viral-associated molecular patterns.	recent work demonstrated that a splice variant of a human macrophage voltage-gated sodium channel expressed on endosomes acts as an intracellular sensor for dsrna, a viral-associated molecular pattern. here our goal was to identify a candidate gene in a clinically relevant invertebrate model with related cellular and pattern recognition properties. the para gene in drosophila and other insects encodes voltage-gated sodium channels with similar electrophysiological properties to those found in ve ...	2015	26086103
dengue hemorrhagic fever at 60 years: early evolution of concepts of causation and treatment.	during the decade of the 1960s, the epidemiology of a new dengue disease, dengue hemorrhagic fever and dengue shock syndrome (dhf/dss), was described by collaborative research performed by thai scientists from many institutions and by workers at the u.s. army's seato medical research laboratory in bangkok, thailand. careful clinical and physiological studies provided the initial description of dss. dss cases were caused by each of the four dengue viruses (denv) and not chikungunya (chik) virus o ...	0	26085471
larvicidal activity of magnolia denudata seed hydrodistillate constituents and related compounds and liquid formulations towards two susceptible and two wild mosquito species.	anopheles sinensis, aedes aegypti and aedes albopictus and culex pipiens pallens mosquitoes transmit malaria, dengue fever and west nile virus diseases respectively. this study was conducted to determine the toxicity of 17 constituents from magnolia denudata seed hydrodistillate (md-shd) and four experimental md-shd liquid formulations (10-50 mg l(-1) liquid) to third-instar larvae from insecticide-susceptible cx. p. pallens and ae. aegypti as well as wild ae. albopictus and an. sinensis.	2016	26085316
biology of zika virus infection in human skin cells.	zika virus (zikv) is an emerging arbovirus of the flaviviridae family, which includes dengue, west nile, yellow fever, and japanese encephalitis viruses, that causes a mosquito-borne disease transmitted by the aedes genus, with recent outbreaks in the south pacific. here we examine the importance of human skin in the entry of zikv and its contribution to the induction of antiviral immune responses. we show that human dermal fibroblasts, epidermal keratinocytes, and immature dendritic cells are p ...	2015	26085147
overexpression of tyrosine hydroxylase and dopa decarboxylase associated with pupal melanization in spodoptera exigua.	melanism has been found in a wide range of species, but the molecular mechanisms involved remain largely elusive. in this study, we studied the molecular mechanisms of the pupal melanism in spodoptera exigua. the full length cdna sequences of tyrosine hydroxylase (th) and dopa decarboxylase (ddc), two key enzymes in the biosynthesis pathway of melanin, were cloned, and their temporal expression patterns in the integument were compared during the larval-pupal metamorphosis process of the s. exigu ...	2015	26084938
rna-seq based phylogeny recapitulates previous phylogeny of the genus flaveria (asteraceae) with some modifications.	the genus flaveria has been extensively used as a model to study the evolution of c4 photosynthesis as it contains c3 and c4 species as well as a number of species that exhibit intermediate types of photosynthesis. the current phylogenetic tree of the genus flaveria contains 21 of the 23 known flaveria species and has been previously constructed using a combination of morphological data and three non-coding dna sequences (nuclear encoded ets, its and chloroplast encoded trnl-f).	2015	26084484
cross-sectional community-based study of the socio-demographic factors associated with the prevalence of dengue in the eastern part of sudan in 2011.	dengue is caused by an arthropod-borne flavivirus. infection can be either primary or secondary based on serology, with each stage of the disease characterized by specific serological conversion and antibody formation. further study is needed to fully identify the factors associated with and predisposing to dengue infection. the objective of this study was to identify socio-demographic factors associated with the prevalence of dengue serotypes in kassala state in the eastern part of sudan in 201 ...	2015	26084275
impacts of climate change on vector borne diseases in the mediterranean basin - implications for preparedness and adaptation policy.	the mediterranean region is vulnerable to climatic changes. a warming trend exists in the basin with changes in rainfall patterns. it is expected that vector-borne diseases (vbd) in the region will be influenced by climate change since weather conditions influence their emergence. for some diseases (i.e., west nile virus) the linkage between emergence andclimate change was recently proved; for others (such as dengue) the risk for local transmission is real. consequently, adaptation and preparati ...	2015	26084000
infection of an insect vector with a bacterial plant pathogen increases its propensity for dispersal.	the spread of vector-transmitted pathogens relies on complex interactions between host, vector and pathogen. in sessile plant pathosystems, the spread of a pathogen highly depends on the movement and mobility of the vector. however, questions remain as to whether and how pathogen-induced vector manipulations may affect the spread of a plant pathogen. here we report for the first time that infection with a bacterial plant pathogen increases the probability of vector dispersal, and that such movem ...	2015	26083763
changes in the gene expression profiles of the hypopharyngeal gland of worker honeybees in association with worker behavior and hormonal factors.	the hypopharyngeal glands (hpgs) of worker honeybees undergo physiological changes along with the age-dependent role change from nursing to foraging: nurse bee hpgs secrete mainly major royal jelly proteins, whereas forager hpgs secrete mainly α-glucosidase iii, which converts the sucrose in the nectar into glucose and fructose. we previously identified two other genes, apis mellifera buffy (ambuffy) and apis mellifera matrix metalloproteinase 1 (ammmp1), with enriched expression in nurse bee an ...	2015	26083737
differential analysis of the secretome of wrl68 cells infected with the chikungunya virus.	the chikungunya virus (chikv) is an arthropod borne virus. in the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. there is limited understanding regarding the underlying molecular mechanisms involved in chikv replication and how the virus interacts with its host. in the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early chikv infection. two-dimensional gel electr ...	2015	26083627
circumsporozoite protein as a potential target for antimalarials.	since the discovery of circumsporozoite protein (csp), a major sporozoite surface antigen, by ruth nussenzweig and victor nussenzweig in the early 1980s, the role of csp in protection against malaria has been extensively investigated. several monoclonal antibodies against csp have been generated to date, with some of them mediating antimalarial protection upon passive transfer into animals. genetically engineered transgenic mosquitoes producing the anti-csp antibody have recently been generated ...	2015	26081442
localization and role of inward rectifier k(+) channels in malpighian tubules of the yellow fever mosquito aedes aegypti.	malpighian tubules of adult female yellow fever mosquitoes aedes aegypti express three inward rectifier k(+) (kir) channel subunits: aekir1, aekir2b and aekir3. here we 1) elucidate the cellular and membrane localization of these three channels in the malpighian tubules, and 2) characterize the effects of small molecule inhibitors of aekir1 and aekir2b channels (vu compounds) on the transepithelial secretion of fluid and electrolytes and the electrophysiology of isolated malpighian tubules. usin ...	2015	26079629
assessment of arbovirus surveillance 13 years after introduction of west nile virus, united states.	before 1999, the united states had no appropriated funding for arboviral surveillance, and many states conducted no such surveillance. after emergence of west nile virus (wnv), federal funding was distributed to state and selected local health departments to build wnv surveillance systems. the council of state and territorial epidemiologists conducted assessments of surveillance capacity of resulting systems in 2004 and in 2012; the assessment in 2012 was conducted after a 61% decrease in federa ...	0	26079471
identification and knockdown of the olfactory receptor (orco) in gypsy moth, lymantria dispar.	the gypsy moth, lymantria dispar, is an important economic pest that causes large-scale damage to forests worldwide. because of its important role in initiating and controlling insect behavior, olfaction-and olfaction-based pest management-has drawn increasing attention from entomologists. in this study, we identified the gene that encodes the olfactory receptor co-receptor (orco). through amino acid sequence alignment, we found that ldisorco shares high identity with other orco proteins from di ...	2015	26078719
characterization of anopheles gambiae (african malaria mosquito) ferritin and the effect of iron on intracellular localization in mosquito cells.	ferritin is a 24-subunit molecule, made up of heavy chain (hc) and light chain (lc) subunits, which stores and controls the release of dietary iron in mammals, plants, and insects. in mosquitoes, dietary iron taken in a bloodmeal is stored inside ferritin. our previous work has demonstrated the transport of dietary iron to the ovaries via ferritin during oogenesis. we evaluated the localization of ferritin subunits inside ccl-125 [aedes aegypti linnaeus (diptera: culicidae), yellow fever mosquit ...	2015	26078302
complete dosage compensation in anopheles stephensi and the evolution of sex-biased genes in mosquitoes.	complete dosage compensation refers to hyperexpression of the entire x or z chromosome in organisms with heterogametic sex chromosomes (xy male or zw female) in order to compensate for having only one copy of the x or z chromosome. recent analyses suggest that complete dosage compensation, as in drosophila melanogaster, may not be the norm. there has been no systematic study focusing on dosage compensation in mosquitoes. however, analysis of dosage compensation in anopheles mosquitoes provides o ...	2015	26078263
asymmetric effects of native and exotic invasive shrubs on ecology of the west nile virus vector culex pipiens (diptera: culicidae).	exotic invasive plants alter the structure and function of native ecosystems and may influence the distribution and abundance of arthropod disease vectors by modifying habitat quality. this study investigated how invasive plants alter the ecology of culex pipiens, an important vector of west nile virus (wnv) in northeastern and midwestern regions of the united states.	2015	26076589
function and evolution of micrornas in eusocial hymenoptera.	the emergence of eusociality ("true sociality") in several insect lineages represents one of the most successful evolutionary adaptations in the animal kingdom in terms of species richness and global biomass. in contrast to solitary insects, eusocial insects evolved a set of unique behavioral and physiological traits such as reproductive division of labor and cooperative brood care, which likely played a major role in their ecological success. the molecular mechanisms that support the social reg ...	2015	26074950
rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant.	most plant viruses that seriously damage agricultural crops are transmitted by insects. however, the mechanisms enabling virus transmission by insect vectors are poorly understood. the brown planthopper (nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including rice ragged stunt virus (rrsv). in this study, we investigated the mechanisms of rrsv transmission from its insect vector to the rice p ...	2015	26073458
evidence of two mechanisms involved in bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: genome dynamic and toxins stability.	biopesticides based on bacillus thuringiensis israelensis are the most used and most successful around the world. this bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a decrease in its effectiveness. the detection of such phenomena is of great importance to monitor the stability of b. thuringiensis strains in industrial production processes of biopesticides. new local b. thuringiensis israelensis isolates were investigated. they present variabl ...	2015	26070692
development of a hamster model for chikungunya virus infection and pathogenesis.	chikungunya virus is transmitted by mosquitoes and causes severe, debilitating infectious arthritis in humans. the need for an animal model to study the disease process and evaluate potential treatments is imminent as the virus continues its spread into novel geographic locations. golden hamsters (mesocricetus auratus) are often used as outbred laboratory animal models for arboviral diseases. here we demonstrate that hamsters inoculated with chikungunya virus developed viremia and histopathologi ...	2015	26070211
relationship between insecticide resistance and kdr mutations in the dengue vector aedes aegypti in southern china.	aedes aegypti is an important vector for dengue virus and thus has been targeted with pyrethroid insecticides in many areas of the world. as such, resistance has been detected to several of these insecticides, including in china, but the mechanisms of the resistance are not well understood in this country.	2015	26068925
eilat virus induces both homologous and heterologous interference.	most alphaviruses are mosquito-borne and exhibit a broad host range, infecting many different vertebrates including birds, rodents, equids, and humans. occasionally, alphaviruses can spill over into the human population and cause disease characterized by debilitating arthralgia or fatal encephalitis. recently, a unique alphavirus, eilat virus (eilv), was described that readily infects mosquito but not vertebrate cell lines. here, we investigated the ability of eilv to induce superinfection exclu ...	2015	26068885
distinct sets of piwi proteins produce arbovirus and transposon-derived pirnas in aedes aegypti mosquito cells.	the piwi-interacting rna (pirna) pathway is essential for transposon silencing in many model organisms. its remarkable efficiency relies on a sophisticated amplification mechanism known as the ping-pong loop. in alphavirus-infected aedes mosquitoes, pirnas with sequence features that suggest ping-pong-dependent biogenesis are produced from viral rna. the piwi family in aedes mosquitoes is expanded when compared to other model organisms, raising the possibility that individual piwi proteins have ...	2015	26068474
conidia of the insect pathogenic fungus, metarhizium anisopliae, fail to adhere to mosquito larval cuticle.	adhesion of conidia of the insect pathogenic fungus, metarhizium anisopliae, to the arthropod host cuticle initially involves hydrophobic forces followed by consolidation facilitated by the action of extracellular enzymes and secretion of mucilage. gene expression analysis and atomic force microscopy were used to directly quantify recognition and adhesion between single conidia of m. anisopliae and the cuticle of the aquatic larval stage of aedes aegypti and a representative terrestrial host, te ...	2014	26064542
intricate predatory decisions by a mosquito-specialist spider from malaysia.	paracyrba wanlessi is a southeast asian jumping spider (salticidae) that lives in the hollow internodes of fallen bamboo and preys on the larvae, pupae and adults of mosquitoes. in contrast to evarcha culicivora, an east african salticid that is also known for actively targeting mosquitoes as preferred prey, there was no evidence of p. wanlessi choosing mosquitoes on the basis of species, sex or diet. however, our findings show that p. wanlessi chooses mosquitoes significantly more often than a ...	2014	26064534
entomological indicators during transmission season of dengue in silvassa (india).	the entomological surveillance was conducted in urban, semi-urban/slum, industrial and residential areas during main transmission period from june to november 2012. in residential sites house index was 41.7-35.0, breteau index 71.7-136.7 and container index 11.6-20.2. during transmission period all the values ware much higher than the threshold level. the causes of high values of entomological indicator appeared to be rapid industrialization, unawareness of the conditions or factors that can exa ...	2013	26064033
an unusual role for doublesex in sex determination in the dipteran sciara.	the gene doublesex, which is placed at the bottom of the sex-determination gene cascade, plays the ultimate discriminatory role for sex determination in insects. in all insects where this gene has been characterized, the dsx premessenger rna (pre-mrna) follows a sex-specific splicing pattern, producing male- and female-specific mrnas encoding the male-dsxm and female-dsxf proteins, which determine male and female development, respectively. this article reports the isolation and characterization ...	2015	26063659
green-synthesized silver nanoparticles as a novel control tool against dengue virus (den-2) and its primary vector aedes aegypti.	dengue is an arthropod-borne viral infection mainly vectored through the bite of aedes mosquitoes. recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depends on effective vector control measures. in this study, we proposed the green-synthesis of silver nanoparticles (agnp) as a novel and eff ...	2015	26063530
electrophysiological and behavioral characterization of bioactive compounds of the thymus vulgaris, cymbopogon winterianus, cuminum cyminum and cinnamomum zeylanicum essential oils against anopheles gambiae and prospects for their use as bednet treatments.	laboratory and field studies showed that repellent, irritant and toxic actions of common public health insecticides reduce human-vector contact and thereby interrupt disease transmission. one of the more effective strategies to reduce disease risk involves the use of long-lasting treated bednets. however, development of insecticide resistance in mosquito populations makes it imperative to find alternatives to these insecticides. our previous study identified four essential oils as alternatives t ...	2015	26063119
evaluation of the toxicity and repellence of an organic fatty acids mixture (c8910) against insecticide susceptible and resistant strains of the major malaria vector anopheles funestus giles (diptera: culicidae).	malaria vector control relies principally on the use of insecticides, especially pyrethroids. because of the increasing occurrence of insecticide resistance in target vector populations, the development of new insecticides, particularly those with novel modes of action, is particularly important, especially in terms of managing insecticide resistance. the c8910 formulation is a patented mixture of compounds comprising straight-chain octanoic, nonanoic and decanoic saturated fatty acids. this com ...	2015	26062763
a defensin from the model beetle tribolium castaneum acts synergistically with telavancin and daptomycin against multidrug resistant staphylococcus aureus.	the red flour beetle tribolium castaneum is a common insect pest and has been established as a model beetle to study insect development and immunity. this study demonstrates that defensin 1 from t. castaneum displays in vitro and in vivo antimicrobial activity against drug resistant staphylococcus aureus strains. the minimum inhibitory concentration (mic) of defensin 1 against 11 reference and clinical staphylococcal isolates was between 16-64 μg/ml. the putative mode of action of the defensin p ...	2015	26062137
the chikungunya virus capsid protein contains linear b cell epitopes in the n- and c-terminal regions that are dependent on an intact c-terminus for antibody recognition.	chikungunya virus (chikv) is an arthropod-borne agent that causes severe arthritic disease in humans and is considered a serious health threat in areas where competent mosquito vectors are prevalent. chikv has recently been responsible for several millions of cases of disease, involving over 40 countries. the recent re-emergence of chikv and its potential threat to human health has stimulated interest in better understanding of the biology and pathogenesis of the virus, and requirement for impro ...	2015	26061335
the spatiotemporal trajectory of a dengue epidemic in a medium-sized city.	understanding the transmission dynamics of infectious diseases is important to allow for improvements of control measures. to investigate the spatiotemporal pattern of an epidemic dengue occurred at a medium-sized city in the northeast region of brazil in 2009, we conducted an ecological study of the notified dengue cases georeferenced according to epidemiological week (ew) and home address. kernel density estimation and space-time interaction were analysed using the knox method. the evolution o ...	2015	26061236
recent advances in dengue: relevance to puerto rico.	dengue represents an increasingly important public health challenge in puerto rico, with recent epidemics in 2007, 2010, and 2012-2013. although recent advances in dengue vaccine development offer hope for primary prevention, the role of health professionals in the diagnosis and management of dengue patients is paramount. case definitions for dengue, dengue with warning signs, and severe dengue provide a framework to guide clinical decision-making. furthermore, the differentiation between dengue ...	0	26061055
abundant aedes (stegomyia) aegypti aegypti mosquitoes in the 2014 dengue outbreak area of mozambique.	in early 2014, dengue cases were reported from northern mozambique, 30 years after the last outbreak. we identified potential dengue vector species in three northern towns, pemba, nampula and nacala, and one southern town, maputo, during the outbreak in april 2014. a major dengue vector species, aedes (stegomyia) aegypti, was found in all these towns. the dominant vector subspecies in the northern towns was aedes aegypti aegypti, while ae. aegypti formosus was dominant in maputo. considering the ...	2015	26060423