Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| mode of action of antimalarial drugs. | chloroquine, quinine, quinacrine and related drugs are effective antimalarial agents only against parasites that degrade haemoglobin. this fact prompted an examination of the role of ferriprotoporphyrin ix (fp), a product of haemoglobin degradation, in the mode of action of chloroquine. fp was identified as a high affinity drug receptor of malaria parasites by showing that it has the appropriate affinity for chloroquine, with a dissociation constant on the order of 10(-8) m, and specificity for ... | 1983 | 6341003 |
| protective immunity to malaria and anti-erythrocyte autoimmunity. | the intraerythrocytic development of malaria parasites results in considerable modification and destruction of erythrocytes. this may lead to the breaking of tolerance such that immune recognition of 'self' or 'modified self' erythrocyte antigens by b or t lymphocytes occurs. such recognition may be a vital factor in the induction of protective immunity even though it may also cause immunopathology. serological and immunocytochemical assays have been used to demonstrate, in the serum of plasmodi ... | 1983 | 6340999 |
| antiparasitic activity of natural and semisynthetic monensin urethanes. | a total of 17 monensin urethanes, including two naturally produced phenethylurethanes (streptomyces sp.), were evaluated for antiparasitic activity. all of the compounds had the characteristic properties of the polyether antibiotics including their ability to transport cations across membranes. several of the semisynthetic derivatives demonstrated in vitro and in vivo anticoccidial activity in chickens. in vitro activity was shown utilizing chick kidney cell culture experimental procedures in wh ... | 1983 | 6340991 |
| enhanced expression of h-2k and h-2d antigens on reticulocytes infected with plasmodium yoelii. | the 17xnl strain of plasmodium yoelii induces a highly effective and permanent t-cell dependent immunity in mice of the cba strain; the lethal variant p. yoelii 17xl and p. berghei (anka) fail to activate an effective immune response in the same host. these differences in immunogenicity are unexplained. we recently observed that in cba/caj mice the intracellular blood stages of p. yoelii 17xnl were almost exclusively within reticulocytes whereas lethal p. yoelii 17xl and p. berghei (anka), at co ... | 1983 | 6339952 |
| malaria parasites--discovery of the early liver form. | infections of mammalian malaria parasites start when sporozoites from an infected anopheline mosquito are injected into the bloodstream of the host. the sporozoites enter the hepatocytes and become transformed into exoerythrocytic schizonts. since the discovery of the primate parasite plasmodium cynomolgi in monkey hepatocytes and the rodent parasite plasmodium berghei in hamster hepatocytes, the ultrastructure of these stages has been extensively studied both in primate and rodent plasmodia. th ... | 1983 | 6339945 |
| 2-acetylpyridine thiosemicarbazones. 5. 1-[1-(2-pyridyl)ethyl]-3-thiosemicarbazides as potential antimalarial agents. | reduction of the azomethine bond of 2-acetylpyridine thio- and selenosemicarbazones with sodium borohydride readily afforded the corresponding thio- or selenosemicarbazides when they were n4,n4-disubstituted. this conversion failed, however, when the thio- or selenosemicarbazones were n4-substituted or unsubstituted. a more general route to the desired thio- or selenosemicarbazides consisted of reduction with sodium borohydride of methyl 3-[1-(2-pyridyl)ethylidene]hydrazinecarbodithioate to give ... | 1983 | 6338234 |
| malaria renders mice susceptible to mosquito feeding when gametocytes are most infective. | mice infected with plasmodium berghei, p. chabaudi, or p. yoelii became lethargic and ceased to display normal antimosquito behavior. periods of reduced defensiveness corresponded with maximum mosquito engorgement and with periods of maximum gametocyte infectivity to mosquitoes. increased feeding success of mosquitoes during periods of peak gametocyte infectivity may be important to the natural maintenance of these malaria parasites. | 1983 | 6338190 |
| structural similarities among the protective antigens of sporozoites from different species of malaria parasites. | 1983 | 6338017 | |
| composition and kinetics of immune complexes in mice infected with plasmodium berghei. | 1983 | 6337777 | |
| endomyocardial lesion and endomyocardial fibrosis in experimental malaria (plasmodium berghei) in mice. | 1983 | 6337776 | |
| pathogenesis of cerebral malaria in golden hamsters and inbred mice. | 1983 | 6337774 | |
| serological reactivity of in vitro cultured exoerythrocytic stages of plasmodium berghei in indirect immunofluorescent or immunoperoxidase antibody tests. | exoerythrocytic (ee) stages of plasmodium berghei were cultivated in vitro in wi38 cells inoculated with sporozoites, and examined for serological reactivity by indirect immunofluorescent or immunoperoxidase tests. at 24 hours post-inoculation, sporozoite and red blood cell (rbc) stage antigens were equally distributed, but by 48 hours, rbc stage antigens predominated. merozoites, produced by 72 hours, reacted strongly with anti-rbc stage sera, but were weakly reactive with anti-sporozoite sera. ... | 1983 | 6337518 |
| plasmodium berghei: electron spin resonance and lipid analysis of infected mouse erythrocyte membranes. | red blood cells from mice infected with plasmodium berghei and from uninfected mice were labeled with stable, free radical derivatives of stearic acid. electron spin resonance spectra of these samples showed that the degree of molecular order in these membranes decreased, and the rate of motion of the probe increased, with increasing levels of parasitemia. parasitemia increased the ratio of unsaturated to saturated 18-carbon fatty acids, and decreased the percentage of arachidonic acid and of ch ... | 1984 | 6325227 |
| ketoconazole and other potent antimycotic azoles exhibit pronounced activity against trypanosoma cruzi, plasmodium berghei and entamoeba histolytica in vivo. | 1984 | 6320547 | |
| [higher microsomal monooxygenase activity in chloroquine-resistant strains of malarial plasmodium as a possible cause of drug stability]. | ability to hydroxylate benz(alpha)pyrene--a substrate of arylhydrocarbone hydroxylase (ahh) was distinctly increased in lysates of erythrocytes containing malarial plasmodium. hydroxylation of benz(alpha)pyrene was inhibited by methyrapone--an inhibitor of microsomal monooxygenases. activity of ahh was increased from 2- to 3-fold in chloroquine-resistant plasmodium strains as compared with the drug-sensitive strains. resistance of plasmodium berghei to chloroquine appears to involve an activatio ... | 1983 | 6316663 |
| further studies and electron microscopic characterization of plasmodium berghei dna. | the average length and the interspersion pattern of repetitive dna sequences in the plasmodium berghei genome have been studied by electron microscopy. within the limitations posed by the relatively high genome complexity, analysis of partially renatured total dna indicates that repetitive sequences do not occupy preferential positions along the genome, but are widely dispersed (one in approx. 8000 base pairs of unique dna). structures appearing as loops flanked by inverted repeats are present. ... | 1983 | 6314137 |
| the enzymes of pyrimidine biosynthesis in babesia bovis and babesia bigemina. | all six enzymes of de novo pyrimidine biosynthesis leading to the formation of ump have been demonstrated in whole homogenates from two bovine babesia species, b. bovis and b. bigemina. the specific activities of the respective enzymes were of the same order of magnitude as observed for the related parasite, plasmodium berghei. the results indicate that both these parasites have the potential of obtaining their pyrimidine requirements by de novo synthesis. subcellular fractionation established t ... | 1983 | 6309129 |
| membrane-associated phosphoproteins in plasmodium berghei-infected murine erythrocytes. | normal and plasmodium berghei (nyu-2 strain)-infected murine erythrocytes display substantially different patterns of plasma membrane phosphoproteins phosphorylation. intact erythrocytes (normal and parasite infected) incubated with 32pi and isolated washed erythrocyte plasma membranes incubated with gamma-32p-atp were analyzed for phosphoproteins by sds page and autoradiography. two new phosphoproteins of molecular weight 45,000 (pp45) and 68,000 (pp68), which are absent in normal erythrocyte m ... | 1983 | 6306014 |
| the four ribosomal dna units of the malaria parasite plasmodium berghei. identification, restriction map, and copy number analysis. | the four ribosomal rna genes (rdna units) of the rodent malaria parasite, plasmodium berghei, were identified and mapped by restriction enzyme analysis and southern blot hybridization of genomic dna. although the four genes share common characteristics, they appear to be internally different from each other in expanse and sequence. one hindiii site near the 3' end of the coding region for the large rrna is the only restriction site which we have detected that is conserved in all of the genes. th ... | 1983 | 6304071 |
| increased fluidity of plasmodium berghei-infected mouse red blood cell membranes detected by electron spin resonance spectroscopy. | 1983 | 6302505 | |
| synthesis and biological properties of 2'-deoxy-5-vinyluridine and 2'deoxy-5-vinylcytidine. | rapid and efficient syntheses for the preparation of 2'-deoxy-5-vinyluridine and 2'-deoxy-5-vinylcytidine are described starting from nucleoside precursors. contrary to some previous reports, 2-deoxy-5-vinyluridine has be found to be quite stable under normal laboratory conditions but when tested in animals shows neither toxicity nor anti-leukemic (l1210 cells) or anti-parasitic (plasmodium berghei) activity. 2'-deoxy-5-vinylcytidine appears to polymerise readily. it is much less toxic to cell c ... | 1982 | 6292837 |
| myristate-induced release of superoxide and hydrogen peroxide from peritoneal macrophages in mice immunized to toxoplasma gondii and plasmodium berghei. | 1982 | 6290731 | |
| mechanism of diethyl-dithiocarbamate induced elevation of parasitemia in plasmodium berghei infection. | 1982 | 6282744 | |
| approaches to assessing host resistance. | there is increasing evidence that chronic, subclinical exposure to certain environmental pollutants may upset immune responsiveness and alter susceptibility of animals to infectious agents. environmental chemicals or drugs may affect diverse aspects of the immune system, leading to immunosuppression, immunopotentiation, hypersensitivity or perturbed innate host resistance. a variety of infectious models is available that involves relatively well defined target organs and host defense mechanisms; ... | 1982 | 6277617 |
| comparative studies on dihydroorotate dehydrogenase from p. berghei and the mouse reticulocyte. | kinetic parameters on dihydroorotate dehydrogenase (dho-dhase) from the rodent malarial parasite, plasmodium berghei, have been determined. this enzyme, the fourth in de novo pyrimidine biosynthesis, is particulate and is absent in the mature mammalian red cell. the km of the substrate, dihydroorotate, was determined to be 23 microm and the ki values for a number of substrate analogues have been determined. the most potent inhibitor was dihydroazaorotate (ki, 3 microm), 5-azaorotate (ki, 20 micr ... | 1981 | 6271112 |
| cyclic amp metabolism in p. berghei infected murine red cells. | 1981 | 6270696 | |
| a simple method for separation of uninfected erythrocytes from those infected with plasmodium berghei and for isolation of artificially released parasites. | rat erythrocytes infected with plasmodium berghei were disrupted by gentle passage of concanavalin a (con a) agglutinated cells through a 100 mesh stainless steel grid. the free parasites were separated from cell debris, unbroken infected cells, and from uninfected rat erythrocytes on a percoll gradient. the parasites remained morphologically intact, metabolically active, and infective to mice. the parasites were observed by light and electron microscopy. the incorporation of 3h-isoleucine and 3 ... | 1981 | 6261470 |
| pyrimidine biosynthesis in plasmodium berghei. | 1981 | 6260538 | |
| plasmodium berghei: modification of sialic acid on red cells from infected mouse blood. | 1981 | 6257538 | |
| plasmodium yoelii and plasmodium berghei: isolation of infected erythrocytes from blood by colloidal silica gradient centrifugation. | 1980 | 6248355 | |
| long-acting, repository antimalarial agents. duration of protection in mice and monkeys following administration of pyrimethamine pamoate. | the duration of protection from blood-stage malarial challenge following single injections of pyrimethamine pamoate was assessed in mice and monkeys. this duration was dose-related and ranged from several weeks in mice to over 4 months in the monkeys. comparisons with the previously reported repository drugs, cycloguanil pamoate and acedapsone (diacetyldiaminodiphenyl sulfone), in mice demonstrated that pyrimethamine pamoate provides an equal or greater duration of protection. studies with mixtu ... | 1984 | 6236702 |
| [antimalarial activities of hydroxypiperaquine and its phosphate against plasmodium berghei and plasmodium cynomolgi]. | 1984 | 6232823 | |
| changes in the capacity of macrophages and t cells to produce interleukins during murine malaria infection. | interleukin 1 (i1-1) produced by activated macrophages and interleukin 2 (i1-2) released by a subset of t lymphocytes upon antigen or mitogen stimulation are the soluble mediators involved in the mechanism of t-cell activation, proliferation, and differentiation. since these t-cell responses are depressed during malaria infection, the capacity of macrophages to produce i1-1 following lipopolysaccharide (lps) stimulation and that of lymphocytes to release i1-2 upon stimulation with concanavalin a ... | 1984 | 6231107 |
| [7 antimalarials in the treatment of mice experimentally infected with pyronaridine-resistant plasmodium berghei]. | 1983 | 6230874 | |
| anti-lymphocyte antibodies in lethal mouse malaria. ii. induction of an autoantibody specific suppressor t cell by non-lethal p. yoelii. | the anti-lymphocyte autoantibody response to irradiated lethal plasmodium berghei malaria parasites in normal mice was significantly reduced when recipients were pre-treated with splenic t cells from mice recovered from a non-lethal plasmodium yoelii infection. suppression was specific for the autoantibody and did not affect the antibody response to the parasite. experiments involving sequential p. yoelii-p. berghei infections in situ revealed that recovery from p. berghei was possible when the ... | 1983 | 6225580 |
| prevention of cerebral malaria by adoptive transfer of malaria-specific cultured t cells into mice infected with plasmodium berghei. | murine t cell populations specific for plasmodium berghei parasites were generated in vitro from balb/c immune lymph node cells. the malaria-specific t lymphocytes were shown: a) to proliferate specifically in vitro in response to stimulation with p. berghei-infected red blood cells; b) to exhibit the thy-1+, lyt-1+2- cell surface phenotype; c) to provide specific helper activity for an in vitro anti-hapten (tnp) plaque-forming cell antibody response; and d) to protect p. berghei-infected mice f ... | 1983 | 6224859 |
| plasmodium berghei: a mouse model for the "sudden death" and "malarial lung" syndromes. | a mouse model for the "sudden death" and "malarial lung" syndromes is described. mice of the c3h/z strain succumb suddenly approximately 7 days after an infection with plasmodium berghei becomes patent, at a time when parasitemia is still moderate (6 to 8%). death could be shown to be due to anaphylactoid shock, probably induced by soluble immune complexes. increased vascular permeability caused transudation and leakage of serum proteins into the interstitium and the alveoli. the lungs were foun ... | 1983 | 6223835 |
| [synthesis, toxicity and antimalarial effects of bispyroquine]. | 1983 | 6223506 | |
| kinetics of immunosuppression of sporozoite-induced immunity by mycobacterium bovis bcg. | the data reported in this study demonstrate that the vaccination of nih/nmri mice with viable mycobacterium bovis bcg organisms induces a state of immunosuppression that renders the recipient animals incapable of a protective immune response to the malaria sporozoite vaccine. the expression of this altered protective immune response is dependent upon the dosage of the two live vaccines, as well as upon the sequence of their administration. data presented here show that the skin test responses (a ... | 1982 | 6215354 |
| interactions between the intestinal flagellates giardia muris and spironucleus muris and the blood parasites babesia microti, plasmodium yoelii and plasmodium berghei in mice. | in mice infected with the intestinal flagellates giardia muris or spironucleus muris, together with the blood parasites babesia microti or plasmodium yoelii, there is a temporary decrease of flagellate cyst output coincident with the peak of the blood parasite infections, followed by a rapid return to normal levels. this decrease in cyst output is correlated with decreased numbers of trophozoites in the small intestine. the effect on s. muris is more marked than that on g. muris. neither blood p ... | 1982 | 6214756 |
| evolutionary conservation of histidine-rich protein genes and rnas in malaria parasites. | 1984 | 6206515 | |
| determination of nuclear dna of five eucoccidian parasites, isospora (toxoplasma) gondii, sarcocystis cruzi, eimeria tenella, e. acervulina and plasmodium berghei, with special reference to gamontogenesis and meiosis in i. (t.) gondii. | dna contents of individual stages of isospora (toxoplasma) gondii and other eucoccida were measured after feulgen-pararosaniline (so2) staining either by direct microfluorometry or by scanning of microphotographic negatives. frequency distributions were analysed using a computer program based on a mathematical model describing cell division. all stages of i. (t.) gondii, except fertilized macrogametes (2c), contained a haploid amount of dna (1c), indicating that meiosis in i. (t.) gondii occurs ... | 1984 | 6204268 |
| cytochemical studies on nuclear dna of four eucoccidian parasites, isospora (toxoplasma) gondii, eimeria tenella, sarcocystis cruzi and plasmodium berghei. | feulgen-pararosaniline (so2) staining was performed on stages in the life-cycle of isospora (toxoplasma) gondii, eimeria tenella, sarcocystis cruzi and plasmodium berghei. the fluorescence emission of the stained dna in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm (green light) of the same specimens. accurate identification of single cells, and especially discrimination between young schizonts and young gamonts was difficult after feulgen sta ... | 1984 | 6200820 |
| [effect of formate on mouse erythrocytes in vivo and in vitro]. | the effect of formiat was tested in erythrocytes of mice damaged by oxidisation. decrease of heinz's bodies, stabilisation of reduced glutathion, and increase of enzyme activities by referring to an example of superoxidismutase could be identified as a positive effect. possibilities of formiat reactions in the metabolism of red blood cells are discussed. | 1983 | 6200395 |
| [oxidative damage of mice erythrocytes infected with plasmodium berghei]. | by using the model of infection with plasmodium berghei in white mice the attempt was made to explain the oxidative damages of red blood cells as a cause for haemolysis. in addition to a diminished new formation of erythrocytes there was an increased cell lysis under the impact of infection. without any changes of the met-hb-percentage and of heinz bodies an increase of gsh and gssg could be measured. the conclusion was drawn that a damage of red blood cells caused by oxidation may occur by chan ... | 1983 | 6196273 |
| anti-red blood cell autoantibodies induced in rat by plasmodium berghei infection bind to a cross-reacting determinant present also in other cell types. | it has been previously shown that rats injected with red blood cells (rbc) infected with plasmodium berghei make autoantibodies reacting with normal rat rbc as demonstrated by a staphylococcal protein a binding assay. in this study, the serum of the infected rats was found to contain also antibodies to several other types of rat cells (lymphoid, liver, and kidney cells and brain tissue) as well as against rbc and lymphoid cells of mice and sheep, but not of man. rat, mouse, and sheep rbc and rat ... | 1983 | 6191899 |
| ultrastructural studies of a vesicle system associated with endoplasmic reticulum in exo-erythrocytic forms of plasmodium berghei. | fine structural studies of a specialized vesicle system associated with the endoplasmic reticulum (er) of exo-erythrocytic plasmodium berghei suggest that this system may be the equivalent of a golgi apparatus. patches of er, randomly distributed in the cytoplasm of developing parasites, are formed of smooth and ribosome-studded cisternae intermingled with each other. the vesicle systems are located between as well as at the edges of er aggregates and appear to be in different stages of budding ... | 1983 | 6191028 |
| the influence of dietary protein on the development of malaria. | in the course of malarial infection in an animal model, the dietary level of protein proved to be important. synthetic diets were used, identical in every respect other than the type and amount of protein. reducing the protein content of the diet led to a decrease in the level of infection and a protein-free diet almost totally suppressed the disease. these findings were obtained in rats infected with either plasmodium berghei or plasmodium vinckei; when the dietary protein was in the form of ca ... | 1981 | 6185057 |
| [protective action of specific gamma globulin against malaria caused by plasmodium berghei]. | 1982 | 6179147 | |
| the use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of plasmodium berghei. | intraerythrocytic parasites of plasmodium vinckei and plasmodium berghei were separated according to their developmental stages using discontinuous percoll gradients. contaminating nucleated blood cells such as leukocytes were removed by elutriation centrifugation. the stages were unequivocally identified in smears using a newly developed dna-specific staining procedure with mithramycin and fluorescence microscopy. this stain can also be used to detect parasites in human blood of very low parasi ... | 1982 | 6177116 |
| plasmodium berghei: immunologically active proteins on the sporozoite surface. | 1982 | 6174361 | |
| [study of the glycolysis of erythrocytes by the method of automatic titration of lactic acid]. | 1981 | 6172639 | |
| protective reaction against malaria infection in mice sensitized with frozen-thawed toxoplasma tachyzoites. | mice sensitized with frozen-thawed toxoplasma antigen emulsified in freund's incomplete adjuvant (fia) showed high resistance against plasmodium berghei infection, but not in mice sensitized with paraformaldehyde-fixed. toxoplasma or saline plus fia. however, mice which survived from malaria infection did not show any protective reaction against the rh strain inoculation. furthermore, an immune interferon activity was detected in the supernatant of the spleen cells collected from the mice sensit ... | 1981 | 6171952 |
| immunodepression of thymus-independent response to dextran in mouse malaria. | the thymus-independent antibody response to the alpha 1-6 epitope of dextran b512 was depressed strongly during acute non-lethal plasmodium yoelii yoelii malaria, but not during low-grade chronic plasmodium berghei infection. in the acute infection, which is self-limiting, the duration of severe immunodepression was short and was seen only in mice immunized at or around the time of peak parasitaemia. mice primed at this time responded normally to challenge 20 days later: thus the primary exposur ... | 1981 | 6167386 |
| monoclonal antibodies to stage-specific, species-specific, and cross-reactive antigens of the rodent malarial parasite, plasmodium yoelii. | eighteen hybridoma cell lines were used to study species-specific, stage-specific, and serological cross-reactive antigens of the rodent malarial parasite, plasmodium yoelii. specificity and location of plasmodial antigens were determined by indirect fluorescent-antibody analysis. results showed that a minimum of 12 distinct plasmodial antigens could be distinguished by the 18 hybridomas. antigens were found on the surface or within the cytoplasm of the parasite, but not on the surface of erythr ... | 1981 | 6166558 |
| inhibition of p. falciparum growth in human erythrocytes by monoclonal antibodies. | malaria is increasing in incidence and prevalence in most tropical areas and is a major problem for both individuals and communities. current malaria research is aimed at developing vaccines and, for this, it may be useful to define plasmodium antigen(s) related to the development of a protective immune response in the host. monoclonal antibodies have recently been shown to interfere with rodent malaria infection (plasmodium berghei) at the sporozoite or merozoite stage. we have now raised monoc ... | 1981 | 6161311 |
| the human materno-foetal relationship in malaria: i. identification of pigment and parasites in the placenta. | to facilitate investigations of the consequences of malarial infection during human pregnancy, several methods for the recognition of pigment and parasites in the placenta were evaluated. pigment was visualized in infected blood smears and placental tissue using both white light and modified fluorescence microscopy. however, the characteristic pigment dots observed with fluorescent light were also apparent in unstained cryostat and deparaffinized placental sections, and following reaction with i ... | 1980 | 6159702 |
| the dual role of macrophages in the sporozoite-induced malaria infection. a hypothesis. | 1980 | 6156920 | |
| effects of photosensitive chemicals on malaria parasite cells. | 1980 | 6155821 | |
| towards tumor therapy with interferons, part ii. interferons: in vivo effects. | 1980 | 6155160 | |
| [long-term preservation of malarial parasites at the temperature of liquid nitrogen: infectivity and the capacity for gamont formation]. | 1984 | 6148688 | |
| identification of circumsporozoite proteins in individual malaria-infected mosquitoes by western blot analysis. | circumsporozoite (cs) proteins of rodent (plasmodium berghei), simian (p. knowlesi), and human (p. falciparum) malaria parasites extracted from dead and dried mosquitoes have been identified by the western blot (immunoblot) technique. dried mosquitoes which were laboratory-reared and infected with plasmodium or freshly dissected sporozoites were triturated in sample reducing buffer and the extracts electrophoresed in a 10% sds-polyacrylamide gel. after transferring the proteins to nitrocellulose ... | 1984 | 6145742 |
| the importance of disease induced changes in mammalian body temperature to mosquito blood feeding. | laboratory mice infected with rodent malaria (plasmodium berghei or p. chabaudi) or st. louis encephalitis virus (sle) were not hyperthermic during the infection period. however, all infected animals displayed pathogen-specific periods of hypothermia. hamsters infected with p. berghei were hyperthermic on day 7 postinfection (pi) but became hypothermic on day 8 pi and remained so until death, approximately 20 days pi. body temperatures of mice infected with p. yoelii were not significantly diffe ... | 1984 | 6142803 |
| specific identification of plasmodium sporozoites using an indirect fluorescent antibody method. | the specific identification of plasmodial sporozoites is not possible on morphological grounds. this study presents a serological method for the identification of sporozoite species, indicating the suitability of this approach for detection and determination of sporozoites in wild vectors collected from malaria endemic areas. specific antisera and monoclonal antibodies prepared against each of two species of rodent malaria (plasmodium berghei or p. yoelii) were evaluated for their ability to dis ... | 1983 | 6137889 |
| production of monoclonal antibodies by hybridomas sensitized to sporozoites of plasmodium berghei. | hybridoma cell lines, which secreted antibodies directed against either the surface of plasmodium berghei sporozoites or mosquito debris, were produced by fusion of spleen cells of p. berghei sporozoite-immunized mice with p3-x63-ag8 myeloma cells. four cloned antibody-secreting cell lines were successfully established. two of these clones (f9 and g10) were obtained from the fusion of spleen cells from mice that had undergone two immunizations. these clones produced an igm antibody that did not ... | 1982 | 6129292 |
| retention of plasmodium berghei sporozoites within perfused mouse livers. | a mouse liver perfusion model was adapted to evaluate the efficiency of the liver in retaining plasmodium berghei sporozoites. specific numbers of sporozoites were perfused into each liver via a portal vein cannula. the numbers of sporozoites in the perfusate effluent were counted and the percent sporozoite retention calculated. over 95% of sporozoites suspended in medium with plasma were retained in a normal liver following a single passage. sporozoites were seen in sinusoids of perfused livers ... | 1982 | 6122362 |
| inhibition of idiotype--anti-idiotype interaction for detection of a parasite antigen: a new immunoassay. | described in this report is an immunoradiometric assay of general applicability that is based on a new principle: the inhibition of the interaction between monoclonal antibodies by an antigen. the advantages of this assay are that it measures concentrations of single epitopes, purified antigen is not required, and the reagents can be obtained in unlimited amounts and are homogeneous. its features are particularly attractive when the antigen has not been purified and is a minor component of a com ... | 1982 | 6122269 |
| rat malarial glomerulonephritis. an experimental model of post-infectious glomerular injury. | this paper describes the immunopathologic findings in acute malaria-associated glomerulonephritis in the rat. young sprague-dawley rats were infected with plasmodium berghei berghei. the subsequent parasitemia and elevation of circulating clq-reactive immune complexes were transient while the appearance of anti-plasmodial antibody in the serum was persistent. sequential examination of renal tissue and urine revealed the following glomerular alterations: (a) granular, predominantly mesangial depo ... | 1981 | 6117979 |
| the enzymes of pyrimidine biosynthesis in a range of parasitic protozoa and helminths. | the activities of carbamoylphosphate synthase, aspartate transcarbamoylase, dihydroorotase, orotate phosphoribosyl transferase and orotidine-5'-phosphate decarboxylase, five of the six enzymes of pyrimidine biosynthesis, have been measured in crithidia fasciculata, trypanosoma cruzi, leishmania major, trichomonas vaginalis, eimeria tenella, toxoplasma gondii, plasmodium berghei, fasciola gigantica, schistosoma mansoni, hymenolepis diminuta, nippostrongylus brasiliensis and trichuris muris. the m ... | 1981 | 6111750 |
| antimalarial aminoalcohol alternatives to mefloquine. | 1980 | 6106359 | |
| studies on the resistance to single and combined antimalarials in the plasmodium berghei mouse model. | 1980 | 6106358 | |
| inhibition of plasmodium berghei sporozoite invasion of cultured hepatoma cells. | monoclonal antibody to a plasmodium berghei surface antigen blocked sporozoite invasion of cultured human hepatoma cells. such antibodies were of igg1 class. two monoclonals of the igm class, probably reactive with the same antigen, did not neutralize invasion. it appears that the sporozoite surface antigen mediates invasion of hepatoma cells. | 1984 | 6095496 |
| ultrastructure of in vitro cultured exoerythrocytic stage of plasmodium berghei in a hepatoma cell line. | because of difficulties in cultivation of the exoerythrocytic (ee) stages of mammalian malaria parasites, investigation of the development of the ee stages has been hindered as compared to that of the other stages. recently, human hepatoma cells (hepg2-a16) have been shown to be useful for the complete developmental cycle of the ee stage of plasmodium berghei. in order to define the morphological events during this process, we evaluated the ee stages developing from sporozoites in these human he ... | 1984 | 6091467 |
| in vitro infectivity of irradiated plasmodium berghei sporozoites to cultured hepatoma cells. | the invasion of gamma-irradiated plasmodium berghei sporozoites into cultured hepatoma cells and their transformation into trophozoites was similar to invasion and transformation of non-irradiated sporozoites. however, trophozoites from irradiated sporozoites did not further develop into schizonts, but persisted within the cells for up to 3 days. sporozoite surface protective antigen was present in trophozoites from irradiated and non-irradiated sporozoites, suggesting that hepatocyte antigen pr ... | 1984 | 6089599 |
| erythrocyte membrane-bound enzymes in mastomys natalensis during plasmodium berghei infection. | the pattern of activity of certain membrane-associated enzymes was followed in the erythrocytes of plasmodium berghei-infected mastomys natalensis. parasitized erythrocytes were separated from non-parasitized populations by percoll-density gradient centrifugation. the activity of adenylate cyclase was markedly increased while those of atpase, acid phosphatase, beta-glucuronidase and n-acetyl-beta-d-glucosaminidase were considerably decreased in the membrane preparations of parasitized erythrocyt ... | 1984 | 6087778 |
| histochemical observations on the exoerythrocytic malaria parasite plasmodium berghei in rat liver. | enzyme histochemical methods were performed on sporozoite infected liver tissue of rats in order to gain insight into the nutrition and metabolism of exoerythrocytic forms of plasmodium berghei. the following enzymes were demonstrated in the hepatocytic stages of the parasites, obtained 41 and 48 h after inoculation of sporozoites: acid phosphatase, cytochrome oxidase, nadh-tetrazolium reductase, succinate dehydrogenase, nad+ and nadp+ dependent isocitrate dehydrogenase, nadp+-dependent malate d ... | 1984 | 6083994 |
| growth of the pre-erythrocytic tissue stages of plasmodium berghei in isolated, perfused tree rat liver. | 1967 | 6066549 | |
| loss of chloroquine resistance on transfer of plasmodium berghei from mouse to hamster. | 1967 | 6062068 | |
| protective immunity produced by the injection of x-irradiated sporozoites of plasmodium berghei. | 1967 | 6057225 | |
| chemotherapy of sporozoite- and blood-induced plasmodium berghei infections with selected antimalarial agents. | 1967 | 6053524 | |
| sequestration of schizonts in the deep tissues of mice infected with chloroquine-resistant plasmodium berghei. | 1967 | 6047763 | |
| differentiation of nuclear and cytoplasmic fine structure during sporogonic development of plasmodium berghei. | 1967 | 6034491 | |
| exotoxin in malaria infection (plasmodium berghei). | 1967 | 6031550 | |
| pathologic physiology and chemotherapy of plasmodium berghei. i. suppression of parasitemia by sulfones and sulfonamides in mice. | 1967 | 6026226 | |
| relations among antimalarial drugs: results of studies with cycloguanil-, sulfone-, or chloroquine-resistant plasmodium berghei in mice. | 1967 | 6021733 | |
| hepatic-lipid changes in mice infected with plasmodium berghei. | 1967 | 6021724 | |
| activity of chloroquine against plasmodium berghei in mice both with and without previous exposure to chloroquine. | 1967 | 6021722 | |
| [does the sex of mice play a role on the influence of temperature on plasmodium berghei infections treated by nivaquine?]. | 1966 | 6014282 | |
| further investigations on resistance and immunity in mice against an infection with plasmodium berghei. | 1966 | 6014217 | |
| [duration of parasitemia (plasmodium berghei) and grade of immunity in swiss mice]. | 1966 | 5999763 | |
| [study of the cycle of plasmodium berghei yoelii in view of the massive production of viable sporozoites and exo-erythrocytary forms]. | 1966 | 5991608 | |
| [the importance of p-aminobenzoic acid to the course and immunity of malaria in animals (plasmodium berghei) and in man (pl. falciparum). i. studies on nmri mice]. | 1966 | 5991298 | |
| [further studies on the significance of the complement fixing antibodies in plasmodium berghei infection of mice]. | 1966 | 5991297 | |
| [cytological aspects in the course of the immunogenetic process in white mice infected by plasmodium berghei]. | 1966 | 5985363 | |
| some observations on the liver cell mitochondria in plasmodium berghei infection. | 1966 | 5981463 | |
| [evaluation of the immunogenic process in plasmodium berghei infections by means of the serum protection test]. | 1966 | 5973712 | |
| eradication of eperythrozoon coccoides with oxophenarsine in normal and drug-resistant lines of plasmodium berghei in mice. | 1966 | 5969107 | |
| drug responses of mepacrine- and primaquine-resistant strains of plasmodium berghei vincke and lips, 1948. | 1966 | 5960105 | |
| fatty acid composition of lipid classes in plasmodium lophurae and plasmodium berghei. | 1966 | 5959096 | |
| effect of agents simulating the abnormalities of the glucose-6-phosphate dehydrogenase-deficient red cell on plasmodium berghei malaria. | 1966 | 5956347 |