Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| influence of fermentable fiber on small intestinal dimensions and transport of glucose and proline in dogs. | to determine whether intestinal dimensions and nutrient absorption are influenced by different types of dietary fiber. | 1999 | 10188819 |
| cdna and genomic cloning of sugar beet v-type h+-atpase subunit a and c isoforms: evidence for coordinate expression during plant development and coordinate induction in response to high salinity. | the plant v-type h+-atpase (v-atpase) does not only serve basic housekeeping functions but is also involved in stress-induced nacl sequestration during salinity stress. to address the question whether the same isoforms conferring housekeeping functions are equally involved in the response to high salinity, we have isolated cdna clones for subunits a and c, as representing the peripheral v1 complex and the membrane-integral v0 complex, respectively, from the halotolerant sugar beet (beta vulgaris ... | 1999 | 10092175 |
| effect of spumol k on the ultrastructure of aspergillus niger strains sensitive or resistant to toxic compounds of beet molasses. | aspergillus niger strains sensitive and resistant to toxic compounds of beet molasses grown in the presence of spumol k were the object of the present studies. the antifoamer used diminished the number of mitochondria in both groups of strains and caused the reduction of their cristae in the sensitive ones. the disturbances in the ultrastructure of nucleus and mitochondria appeared mostly in sensitive strains. on the other hand, spumol k presence in resistant strains not only increased the numbe ... | 1999 | 10091948 |
| complete glycan structure of the s-layer glycoprotein of aneurinibacillus thermoaerophilus gs4-97. | isolate gs4-97 was purified from an extraction juice sample of an austrian beet sugar factory and affiliated to the newly described species aneurinibacillus thermoaerophilus. it is closely related to the type strain of this species, a.thermoaerophilus l420-91(t), and possesses a square surface layer (s-layer) array composed of identical glycoprotein monomers as its outermost cell envelope component. by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified s-layer showed an appa ... | 1999 | 10089215 |
| minor groove rna triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot. | many viruses regulate translation of polycistronic mrna using a -1 ribosomal frameshift induced by an rna pseudoknot. a pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. a 1.6 a crystal structure of the beet western yellow virus (bwyv) pseudoknot reveals rotation and a bend at the junction of the two stems. a loop base is inserted in the major groove of one stem with quadruple-base interactions. the second loop forms a new minor-groove triplex motif with the o ... | 1999 | 10074948 |
| comparison of melibiose utilizing baker's yeast strains produced by genetic engineering and classical breeding. | yeast strains currently used in the baking industry cannot fully utilize the trisaccharide raffinose found in beet molasses due to the absence of melibiase (alpha-galactosidase) activity. to overcome this deficiency, the mel1 gene encoding melibiase enzyme was introduced into baker's yeast by both classical breeding and recombinant dna technology. both types of yeast strains were capable of vigorous fermentation in the presence of high levels of sucrose, making them suitable for the rapidly deve ... | 1999 | 10063644 |
| overexpression of the bacillus thuringiensis (bt) cry2aa2 protein in chloroplasts confers resistance to plants against susceptible and bt-resistant insects. | evolving levels of resistance in insects to the bioinsecticide bacillus thuringiensis (bt) can be dramatically reduced through the genetic engineering of chloroplasts in plants. when transgenic tobacco leaves expressing cry2aa2 protoxin in chloroplasts were fed to susceptible, cry1a-resistant (20,000- to 40,000-fold) and cry2aa2-resistant (330- to 393-fold) tobacco budworm heliothis virescens, cotton bollworm helicoverpa zea, and the beet armyworm spodoptera exigua, 100% mortality was observed a ... | 1999 | 10051556 |
| the structure of plant vacuolar membranes according to infrared spectroscopy. | the structure of the vacuolar membrane (tonoplast) was studied in red beet roots by ir spectroscopy. the vacuolar membrane was shown to be composed of highly ordered lipids which form regions of free liquid lipid bilayer loosely bound to integral proteins. the prevalence of polar lipids in the tonoplast is responsible for the high elasticity and fluidity of the membrane. the presence of alpha-tocopherol in the tonoplast membrane accounts for a high antioxidant activity of the membrane. integral ... | 1998 | 10024971 |
| effects of type of carbohydrate supplementation to lush pasture on microbial fermentation in continuous culture. | eight single-flow continuous culture fermenters were used to study the effects of the type of energy source on ruminal n utilization from high quality pasture. the four dietary treatments included high quality grass and legume pasture alone (50:50; wt/wt), pasture plus soybean hulls, pasture plus beet pulp, and pasture plus corn. diets supplemented with additional sources of energy (soybean hulls, beet pulp, and corn) were isocaloric but differed in the type and rate of carbohydrate fermentation ... | 1999 | 10022017 |
| evaluation of the physical structure of fodder beets, potatoes, pressed beet pulp, brewers grains, and corn cob silage. | the physical structure of fodder beets, raw potatoes, ensiled pressed sugar beet pulp, ensiled brewers grains, and corn cob silage was evaluated in diets containing concentrates and either corn silage or grass silage as the roughage source. in one series of experiments, the chewing activity of eight cows was measured over 4 d. the beet pulp and fodder beets were added to the two roughage sources at two ratios [20:80 and 35:65, dry matter (dm) basis]. potatoes, brewers grains, and corn cob silage ... | 1999 | 10022013 |
| the minor coat protein of beet yellows closterovirus encapsidates the 5' terminus of rna in virions. | filamentous particles of beet yellows closterovirus (byv) are built of two related capsid proteins, of which the minor species, p24, forms a 75 nm tail at one end of the virion. in the present work, we used polyclonal antibodies against p24 for isolating the 'tailed' virion segments from sonicated byv particle preparations. the [gamma-32p]atp-labelled rna obtained from the antibody-selected particle segments consistently showed stronger hybridization with the 5'-terminal byv cdna clones than wit ... | 1999 | 9934711 |
| redox agents regulate ion channel activity in vacuoles from higher plant cells. | the ability of redox agents to modulate certain characteristics of voltage- and calcium-activated channels has been recently investigated in a variety of animal cells. we report here the first evidence that redox agents regulate the activation of ion channels in the tonoplast of higher plants. using the patch-clamp technique, we have demonstrated that, in tonoplasts from the leaves of the marine seagrass posidonia oceanica and the root of the sugar beet, a variety of sulphydryl reducing agents, ... | 1999 | 9928987 |
| the energetic value of nonstarch polysaccharides in relation to physical activity in group-housed, growing pigs. | the dose response effect of dietary nonstarch polysaccharide (nsp) content on physical activity in relation to metabolic rate in pigs was studied. twelve clusters of 14 pigs (50-kg castrated males) were fed one of four diets, similar in composition, except for the starch and nsp content. the difference in starch and nsp ratio was created by exchanging sugar beet pulp silage (sbps) for tapioca. on a dm basis, diets contained 0, 5, 10, or 15% sbps. pigs were housed in groups and fed at 2.5 times t ... | 1998 | 9928606 |
| antisense-mediated depletion of a potato lipoxygenase reduces wound induction of proteinase inhibitors and increases weight gain of insect pests. | de novo jasmonic acid (ja) synthesis is required for wound-induced expression of proteinase inhibitors and other defense genes in potato and tomato. the first step in ja biosynthesis involves lipoxygenase (lox) introducing molecular oxygen at the c-13 position of linolenic acid. we previously have shown that, in potato, at least two gene families code for 13-lox proteins. we have now produced transgenic potato plants devoid of one specific 13-lox isoform (lox-h3) through antisense-mediated deple ... | 1999 | 9927708 |
| alkylfurans: effects of alkyl side-chain length on insecticidal activity. | the insecticidal activity of five alkylfurans against the generalist insect herbivore beet armyworm, spodoptera exigua, was examined. two naturally occurring compounds, the avocadofurans 2-(pentadecyl)furan (1) and 2-(heptadecyl)furan (2), previously isolated from specialized avocado idioblast oil cells, and three homologues, 2-(tetradecyl)furan (3), 2-(hexadecyl)furan (4), and 2-(octadecyl)furan (5), were synthesized. bioassays of alkylfurans 1-5 using a 9-day diet-incorporation initiated with ... | 1999 | 9917319 |
| inhibition of vacuolar ion channels by polyamines. | in this work, direct effects of cytosolic polyamines on the two principle vacuolar ion channels were studied by means of patch-clamp technique. fast and slow activating vacuolar channels were analyzed on membrane patches isolated from vacuoles of the red beet taproot. the potency of the fast and of the slow vacuolar channel blockage by polyamines decreased with a decrease of the polycation charge, spermine4+ > spermidine3+ > putrescine2+. in contrast to the inhibition of the fast vacuolar channe ... | 1999 | 9916144 |
| elevation of conjugated cis-9, trans-11-octadecadienoic acid in bovine milk because of dietary supplementation. | cows on pasture were fed full fat soybeans (toasted, flaked, and pelleted) or ground full fat rapeseeds to investigate effects on cis-9, trans-11-octadecadienoic acid in milk. three herds of 16 cows each that were on pasture were fed 3.1 kg/d of unmolassed beet pulp (control), 3.0 kg/d of rapeseed concentrate, or 3.1 kg/d of a soybean supplement. the concentration of cis-9, trans-11-octadecadienoic acid in the milk of cows fed the rapeseed and soybean supplements was significantly higher than in ... | 1998 | 9891271 |
| flow injection analysis system for the supervision of industrial chromatographic downstream processing in biotechnology. | sugar beet molasses is a natural resource for various products used in daily life, ranging from sucrose to amino acids for pharmaceutical industry. the separation of molasses into these high value components is performed on a large scale by ion exchange/exclusion chromatography. a biosensor system was set up for the "in time" analysis of serine and sucrose during molasses desugarisation. d-serine was analysed with the multi-enzyme system d-serine dehydratase/lactic dehydrogenase and photometric ... | 1998 | 9883558 |
| isolation and stability of histidine-tagged proteins produced in plants via potyvirus gene vectors. | a system for the expression and purification of histidine-tagged proteins from plants has been developed using a tobacco etch potyvirus (tev)-derived gene vectors. the vectors offered a convenient polylinker and a choice of histidine tagging at the recombinant proteins' n or c termini. these vectors were utilized for expression of proteins encoded by beet yellows closterovirus (byv). approximately 4 micrograms/g of 20-kda byv protein was readily isolated from plants systemically infected by hybr ... | 1998 | 9875335 |
| in situ population dynamics of bacterial viruses in a terrestrial environment | predation by bacteriophages is thought to control bacterial numbers and facilitate gene transfer among bacteria in the biosphere. a thorough understanding of phage population dynamics is therefore necessary if their significance in natural environments is to be fully appreciated. here we describe the in situ population dynamics of three separate phage populations predating on separate bacterial species, living on the surface of field-grown sugar beet (beta vulgaris var. amethyst), as recorded ov ... | 1999 | 9872776 |
| ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus. | antisera to the bacterially expressed nonstructural proteins (nsp) hc-pro, ci, nia, and nib and the coat protein (cp) of plum pox potyvirus (ppv) were used for analysing the composition of virus-induced cytoplasmic and nuclear inclusions by electron microscopy. the antisera reacted with nsp and cp of ppv on immunogold-labelled ultrathin sections. antiserum to cp reacted with virions of seven out of 18 other potyviruses. cp was distributed throughout the cytoplasm of infected cells. antisera to p ... | 1998 | 9856098 |
| [pectin production from burdock (arctium sp.)]. | the pectin complex of different morphological parts of burdock: leaf, leaf stems, main stock have been investigated. high quantity of pectic substances about 1.9% per wet mass, has been detected in the leaf stems of burdock. it has been also established that pressed leaf stems as to pectin content (about 21% of dry mass) do not give in to traditional raw material for pectin production: pressed apple, squeezed sugar-beet, citrus fruit peel, sunflower head, etc. the technological scheme of pectin ... | 2015 | 9848148 |
| physico-chemical properties of the female sex pheromone of heterodera schachtii (nematoda: heteroderidae). | several physico-chemical properties of the female sex pheromone of the beet cyst nematode heterodera schachtii were elucidated. at least one component of the pheromone can be extracted from aqueous solutions with diethyl ether. however, the pheromone has a higher solubility in water, as most of the pheromone activity remained in the water fraction. ion exchange chromatography revealed that the pheromone or at least one of its components is positively charged, whereas another pheromone component ... | 1998 | 9846605 |
| oligosaccharides generated by partial hydrolysis of the borate-rhamnogalacturonan ii complex from sugar beet. | the borate-rhamnogalacturonan ii complex (b-rg-ii), isolated from sugar beet (beta vulgaris), was partially acid-hydrolyzed. the oligosaccharides generated were characterized by glycosyl-composition and glycosyl-linkage analyses, es-mass, and nmr spectroscopy. two disaccharides, alpha-l-rhap-(1-->5)-d-kdo and alpha-l-araf-(1-->5)-dha, an aceric acid-containing oligosaccharide, and a 2-o-me-xyl-containing oligosaccharide, in addition to partially methyl-esterified alpha-(1-->4)-oligogalacturonide ... | 1998 | 9842725 |
| properties of a virus causing mosaic and leaf curl disease of celosia argentea l. in nigeria. | a sap transmissible virus, causing mosaic and leaf curl disease of celosia argentea, was isolated at vegetable farms in amuwo odofin, tejuoso, and abule ado, lagos, nigeria. the virus had a restricted host range confined to a few species of the amaranthaceae, chenopodiaceae and solanaceae families. it failed to infect several other species of the aizoaceae, brassicaceae, cucurbitaceae, fabaceae, lamiaceae, malvaceae, poaceae and tiliaceae families. the virus was transmitted in a non-persistent m ... | 1998 | 9842442 |
| accumulation of cadmium from wheat bran, sugar-beet fibre, carrots and cadmium chloride in the liver and kidneys of mice. | the gastrointestinal absorption and organ distribution of cd after exposure for 9 weeks to three fibre-rich foodstuffs (wheat bran, sugar-beet fibre and carrots) were determined in mice. groups of eight mice were given a diet containing 0.05 mg cd/kg from wheat bran, sugar-beet fibre, carrots or cdcl2 mixed in a semi-synthetic, low-cd (< 0.007 mg/kg) feed. a control group was fed on the low-cd semi-synthetic feed. the water consumption, food consumption and the weight of the animals were monitor ... | 1998 | 9828763 |
| relationships among absorbents on the reduction of grass silage effluent and silage quality. | effluent from grass silage is a threat to water quality and a loss of valuable forage nutrients from dairy farms. absorbents potentially reduce effluent loss when weather conditions are not ideal for field wilting. the objective was to determine the effectiveness of various absorbents at reducing silage effluent. first-harvest, direct-cut, perennial grass forage was ensiled with and without absorbents in medium-sized experimental silos for 3 consecutive yr. silos contained 4.54 kg of grass and o ... | 1998 | 9812269 |
| concerted biosynthesis of an insect elicitor of plant volatiles. | a variety of agricultural plant species, including corn, respond to insect herbivore damage by releasing large quantities of volatile compounds and, as a result, become highly attractive to parasitic wasps that attack the herbivores. an elicitor of plant volatiles, n-(17-hydroxylinolenoyl)-l-glutamine, named volicitin and isolated from beet armyworm caterpillars, is a key component in plant recognition of damage from insect herbivory. chemical analysis of the oral secretion from beet armyworms t ... | 1998 | 9811910 |
| positive correlation between recombination rates and levels of genetic variation in natural populations of sea beet (beta vulgaris subsp. maritima). | the relation between the level of genetic variation and the rate of recombination per physical unit was investigated in sea beet (beta vulgaris subsp. maritima). the rate of recombination per physical unit was estimated indirectly through marker density in an rflp linkage map of sugar beet. from this map, we also selected rflp markers covering two of the nine chromosomes in beta. the markers were used to estimate the level of genetic variation in three populations of sea beet, two from italy and ... | 1998 | 9799275 |
| determination of provitamin a of green leafy vegetables by high performance liquid chromatography and open column chromatography. | the purpose of study was to determine the provitamin a value through beta-carotene analysis of five brazilian leafy vegetables: carrot leaves (daucus carota l.), beet leaves (beta vulgaris l.) "serralha" (sonchus oleracea l.), mint (mentha piperita ll.) and celery leaves (aplum graveolens. l.). two analysis methods were used: open-column chromatography (occ) and high performance liquid chromatography (hplc). two analysis methods were used: open-column chromatography (occ) and high performance li ... | 2013 | 9795484 |
| polyhedrin sequence determines the tetrahedral shape of occlusion bodies in thysanoplusia orichalcea single-nucleocapsid nucleopolyhedrovirus. | a nucleopolyhedrovirus (npv) isolated from the looper thysanoplusia orichalcea l. (lepidoptera: noctuidae) (thornpv) is occluded in a tetrahedral protein matrix. the orf of the thornpv polyhedrin gene contains 738 nt which code for 246 amino acids of the putative polyhedrin protein with an estimated molecular mass of 28,778 da. the promoter of this gene is similar in length to the promoter of spodoptera frugiperda npv (sfmnpv), with a 5 nt deletion before the start codon compared to those of oth ... | 1998 | 9780063 |
| specific inclusion bodies are associated with replication of lettuce infectious yellows virus rnas in nicotiana benthamiana protoplasts. | nicotiana benthamiana mesophyll protoplasts, either mock-inoculated or inoculated using in vitro transcripts derived from lettuce infectious yellows virus (liyv) rna 1- and/or rna 2-cloned cdnas were analysed by transmission electron microscopy (tem) and, in some cases, also by immunogold labelling. tem revealed the main cytopathological effects of liyv infections in n. benthamiana protoplasts infected with rnas 1 and 2: (a) typical closterovirus-induced (beet yellows virus-type) accumulations o ... | 1998 | 9780036 |
| the effect of chard (beta vulgaris l. var. cicla) on the skin of streptozotocin induced diabetic rats. | chard (beta vulgaris l. var. cicla) is one of the plants used as hypoglycaemic agent by diabetics in turkey and it has been reported to reduce blood glucose. the purpose of this study was to investigate the effect of feeding chard on diabetes induced impairments in rat skins. uncontrolled induced diabetes caused significant increases in nonenzymatic glycosylation of skin proteins, lipid peroxidation and blood glucose. administration of chard extract inhibited these effects except the increase in ... | 1998 | 9770212 |
| effect of carbon source on production of alpha-l-arabinofuranosidase by aureobasidium pullulans | a color-variant strain of aureobasidium pullulans (nrrl y-12974) produced alpha-l-arabinofuranosidase (alpha-l-afase) when grown in liquid culture on sugar beet arabinan, wheat arabinoxylan, l-arabinose, l-arabitol, xylose, xylitol, oat spelt xylan, corn fiber, or arabinogalactan. l-arabinose was most effective for production of both whole-broth and extracellular alpha-l-afase activity, followed by l-arabitol. oat spelt xylan, sugar beet arabinan, xylose, xylitol, and wheat arabinoxylan were int ... | 1998 | 9767714 |
| a ca2+- and voltage-dependent cation channel in the nuclear envelope of red beet. | the patch-clamp technique was applied to study ion conductances in various configurations of the nuclear envelope of non-enzyme-treated red beet (beta vulgaris l.) nuclei. with excised patches a non-selective cation channel was observed, that was activated by micromolar concentrations of ca2+ on the nucleoplasmic side of the envelope. the channel activity was also voltage-dependent and the voltage threshold of channel activation changed with the nucleoplasmic ca2+ concentration. the most promine ... | 1998 | 9767144 |
| secondary metabolite- and endochitinase-dependent antagonism toward plant-pathogenic microfungi of pseudomonas fluorescens isolates from sugar beet rhizosphere | forty-seven isolates representing all biovars of pseudomonas fluorescens (biovars i to vi) were collected from the rhizosphere of field-grown sugar beet plants to select candidate strains for biological control of preemergence damping-off disease. the isolates were tested for in vitro antagonism toward the plant-pathogenic microfungi pythium ultimum and rhizoctonia solani in three different plate test media. mechanisms of fungal inhibition were elucidated by tracing secondary-metabolite producti ... | 1998 | 9758768 |
| isolation, identification, and biological activity of isopersin, a new compound from avocado idioblast oil cells | a new compound, (12z,15z)-1-hydroxy-4-oxo-heneicosa-12,15-dien-2-yl acetate, isopersin (2), has been isolated from avocado idioblast oil cells. in artificial diet bioassays, 2 showed no effects on either larval survivorship or growth of early-instar beet armyworm spodoptera exigua. in contrast, the isomeric persin (1), (12z, 15z)-1-acetoxy-2-hydroxy-4-oxo-heneicosa-12,15-diene, reduces larval growth at equivalent concentrations (500 &mgr;g g-1). compound 2 is not very stable and isomerizes readi ... | 1998 | 9748393 |
| high level fructan accumulation in a transgenic sugar beet. | we have transformed sugar beet into a crop that produces fructans. the gene encoding 1-sucrose:sucrose fructosyl transferase (1-sst), which was isolated from helianthus tuberosus, was introduced into sugar beet. in h. tuberosus, 1-sst mediates the first steps in fructan synthesis through the conversion of sucrose (gf) into low molecular weight fructans gf2, gf3, and gf4. in the taproot of sugar beet transformed with the 1-sst gene, the stored sucrose is almost totally converted into low molecula ... | 1998 | 9743117 |
| a convert to fructans in sugar beet. | 1998 | 9743111 | |
| characterization of a red beet protein homologous to the essential 36-kilodalton subunit of the yeast v-type atpase. | v-type proton-translocating atpases (v-atpases) (ec 3.6.1.3) are electrogenic proton pumps involved in acidification of endomembrane compartments in all eukaryotic cells. v-atpases from various species consist of 8 to 12 polypeptide subunits arranged into an integral membrane proton pore sector (vo) and a peripherally associated catalytic sector (v1). several v-atpase subunits are functionally and structurally conserved among all species examined. in yeast, a 36-kd peripheral subunit encoded by ... | 1998 | 9742042 |
| protective effects of fruits and vegetables against in vivo clastogenicity of cyclosphosphamide or benzo[a]pyrene in mice. | seven fruits and 10 vegetables commonly consumed in germany were investigated for their anticlastogenic potencies against cyclophosphamide (cp) and benzo[a]pyrene (bap) in the in vivo mouse bone marrow micronucleus assay. we detected protective effects in 76.5% and 70.6% of the samples, respectively, and more or less distinct quantitative differences between the various plant materials and the two clastogens investigated. with respect to cp, moderate activities were exerted by sweet cherries, st ... | 1998 | 9734714 |
| [studies for measuring nitrates and nitrites in food served to patients in hospitals from the province of białystok]. | the aim of this study was determination of daily level intake of nitrates and nitrites in the individual meals in the daily food rations of patients from the hospitals from the province of białystok. the studied material were meals and total diets, with regard to selected food products, which were taken to preparing the dishes. the investigation materials were taken from february to june 1996. analysis of nitrates and nitrites contents was realized by the commonly method based on the reaction of ... | 1998 | 9734230 |
| distinct biochemical and topological properties of the 31- and 27-kilodalton plasma membrane intrinsic protein subgroups from red beet. | plasma membrane vesicles from red beet (beta vulgaris l.) storage tissue contain two prominent major intrinsic protein species of 31 and 27 kd (x. qi, c.y tai, b.p. wasserman [1995] plant physiol 108: 387-392). in this study affinity-purified antibodies were used to investigate their localization and biochemical properties. both plasma membrane intrinsic protein (pmip) subgroups partitioned identically in sucrose gradients; however, each exhibited distinct properties when probed for multimer for ... | 1998 | 9733551 |
| serological characterization of the 3'-proximal encoded proteins of beet yellows closterovirus. | the 3'-proximal open reading frames (orfs) of beet yellows closterovirus, california isolate (byv-ca), were sequenced and the expression of the corresponding proteins analyzed. the nucleotide sequence of orf 5 (coding for p24) was most conserved compared with orf 7 (coding for p20) and orf 8 (coding for p21) among the isolates analyzed. polyclonal antisera were produced to gst fusion proteins of p24, p20, and p21. accumulation of p24, cp, p20 and p21 was studied in infected tetragonia expansa pl ... | 1998 | 9722879 |
| the genome organization of the broad bean necrosis virus (bbnv). | the genome of the broad bean necrosis virus oita-isolate (bbnv-o) [rna1 (6.0 kb), rna2 (2.8 kb) and rna3 (2.4 kb)] was cloned and sequenced. computer analysis indicates that methyltransferase, helicase and rna-dependent rna polymerase (rdrp) motifs are present in rna1. the viral capsid protein (cp) cistron is located at the 5' terminal end of rna2 and the mr of cp (20 k) is close to that determined by sds-page analysis. an ochre codon (uaa) in the cp cistron is thought to be partially suppressed ... | 1998 | 9722878 |
| genome properties of beet virus q, a new furo-like virus from sugarbeet, determined from unpurified virus. | based solely on the information that beet virus q (bvq) contains tubular particles, the entire nucleotide sequence of its tripartite genome was determined from unpurified virus in ca. 40 ml crude sap from locally infected chenopodium quinoa. a starting sequence for rna 1 was generated using primers corresponding to highly conserved helicase domains in the respective rnas of furo-, pomo-, peclu-, hordei- and tobraviruses, and was extended by a walking random-primed cdna approach. the similarity o ... | 1998 | 9714254 |
| analysis of genetic structure and dispersal patterns in a population of sea beet. | a model of the migration pattern in a metapopulation of sea beet (beta vulgaris l. ssp. maritima), based on the continuous distributions of seed and pollen movements, is fitted to gene frequency data at 12 isozyme and rflp loci by maximum likelihood by using an approximation of the simultaneous equilibrium distribution of the gene frequencies generated by the underlying multivariate stochastic process of genetic drift in the population. several alternative restrictions of the general model are f ... | 1998 | 9691051 |
| dietary carbohydrates alter the fecal composition and ph and the ammonia emission from slurry of growing pigs. | we investigated the effects of dietary carbohydrates on the composition and ph of fecal material and on the ammonia emission from the slurry of growing pigs. thirty-four barrows (bw approximately 40 kg) were randomly allotted to 1 of 10 diets. a basal diet was formulated to meet all requirements for protein, amino acids, minerals, and vitamins. the control diet was composed of the basal diet plus heat-treated cornstarch. in the other diets, the cornstarch in the control diet was replaced with th ... | 1998 | 9690645 |
| determination of copper and arsenic in refined beet sugar by stripping voltammetry without sample pretreatment. | copper and arsenic have been analysed in refined beet sugar at the microgram kg-1 level by anodic stripping voltammetry (copper) and cathodic stripping voltammetry (arsenic) in the differential-pulse mode (dpasv and dpcsv) at a hanging mercury drop electrode (hmde). dpcsv measurements of arsenic are based on its accumulation onto the hmde as an intermetallic cu-as compound followed by the reduction of as0 to arsine in hydrochloric acid medium. measurements were directly carried out on untreated ... | 1998 | 9684407 |
| the synthesis of hsps in sugar beet suspension culture cells under hyperthermia exhibits differential sensitivity to calcium. | experiments aimed at testing the effect of ca2+ on heat shock-induced changes in protein synthesis of cultured sugar beet cells were performed. heat shock inhibits the synthesis of non-heat shock proteins (non-hsps) and promotes the synthesis of a set of hsps. extracellular ca2+ appeared to be strictly required for the synthesis of non-hsps. calcium was found to differentially exert its effect on the hsp synthesis: calcium induced (96 and 76 kda), stimulated (94, 67, 58, 52, 32, 30, 26 and 22 kd ... | 1998 | 9678248 |
| a pathogenicity gene isolated from the ppath plasmid of erwinia herbicola pv. gypsophilae determines host specificity. | the host range of the gall-forming bacterium erwinia herbicola pv. gypsophilae (ehg) is restricted to the gypsophila plant whereas e. herbicola pv. betae (ehb) incites galls on beet as well as gypsophila. the pathogenicity of ehg and ehb was previously shown to be dependent on a plasmid (ppath). transposon mutagenesis was used to generate mutants on the cosmid pla150 of the ppath from ehg824-1. a cluster of nonpathogenic mutations flanked by two is1327 elements was identified on a 3.2-kb ndei dn ... | 1998 | 9675891 |
| generation of monoclonal antibody specific to (1-->5)-alpha-l-arabinan. | a neoglycoprotein (a heptasaccharide of (1-->5)-alpha-l-linked-arabinosyl residues linked to bovine serum albumin) has been used to generate a rat monoclonal antibody specific to a linear chain of (1-->5)-alpha-l-arabinan which is a structural feature of the side chains of pectins. the antibody, designated lm6, detected 100 ng of debranched sugar beet arabinan in an immunodot binding assay and 1 microgram of commercial citrus pectin in a similar assay. hapten inhibition studies indicated that th ... | 1998 | 9675359 |
| nicotiana benthamiana plants expressing beet necrotic yellow vein virus (bnyvv) coat protein-specific scfv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection. | transgenic plants of nicotiana benthamiana expressing single chain antibody fragments (scfv) specific for the coat protein of beet necrotic yellow vein virus (bnyvv) and non-expressing control plants were inoculated with bnyvv mechanically and by means of the vector polymyxa betae. the scfv were presumably expressed in the endoplasmic reticulum (er). the average time needed for infections to become detectable was longer in the scfv-expressing plants than in the non-expressing control plants. in ... | 1997 | 9672643 |
| transfer rna mimicry in a new group of positive-strand rna plant viruses, the furoviruses: differential aminoacylation between the rna components of one genome. | recent sequencing of the genomes of several furoviruses--fungus-transmitted rod-shaped positive-strand plant viruses--has suggested the presence of trna-like structures (tlss) at the 3' ends of the genomic rnas. we show here that the genomic rnas of soil-borne wheat mosaic virus (sbwmv), beet soil-borne virus (bsbv), potato mop-top virus (pmtv), peanut clump virus (pcv), and indian peanut clump virus (ipcv) all possess functional tlss that are capable of high-efficiency valylation. while the sbw ... | 1998 | 9657004 |
| cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial dna analysis. | mitochondrial (mt) dna restriction fragment length polymorphisms are convenient markers for identifying cytoplasmic variation among plant cultivars. in an attempt to detect new cytoplasmic genotypes useful for sugarbeet breeding, we have compared the hybridization patterns of mtdna from three groups of cultivated beets, viz. leaf beet, garden beet, and fodder beet. utilized as probes were the two sugarbeet mtdna clones that were capable of distinguishing normal fertile and different sources of m ... | 1998 | 9652232 |
| cell-to-cell movement of beet necrotic yellow vein virus: i. heterologous complementation experiments provide evidence for specific interactions among the triple gene block proteins. | cell-to-cell movement of beet necrotic yellow vein virus (bnyvv) requires three proteins encoded by a triple gene block (tgb) on viral rna 2. a bnyvv rna 3-derived replicon was used to express movement proteins to functionally substitute for the bnyvv tgb proteins was tested by coinoculation of tgb-defective bnyvv with the various replicons to chenopodium quinoa. trans-heterocomplementation was successful with the movement protein (p30) of tobacco mosaic virus but not with the tubule-forming mov ... | 1998 | 9650295 |
| genetic relationships within the genus beta determined using both pcr-based marker and dna sequencing techniques. | the sequences of its1 of the internal transcribed spacer regions of nuclear ribosomal dna from 11 species or subspecies in four sections of the genus beta were compared. phylogeny of these wild beet taxa was inferred from the sequence data using phenetic and phylogenetic analyses. multiple accessions from the same 11 taxa were subjected to random amplified polymorphic dna (rapd) analysis, and the data were analysed phenetically. with both molecular techniques and each analysis, three distinctive ... | 1998 | 9650280 |
| a high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells. | an optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (beta vulgaris l.). a polyethylene glycol-mediated dna transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. the majority of plants were obtained within ... | 1996 | 9631066 |
| the influence of sugar-beet fibre, guar gum and inulin on nutrient digestibility, water consumption and plasma metabolites in healthy beagle dogs. | the aim of the present study was to evaluate the effects of three fibres (sugar-beet fibre, guar gum and inulin) incorporated in the basal diet of healthy dogs at 7 per cent of dry matter (dm). parameters examined included stool output, water consumption, nutrient digestibility and fasting and postprandial plasma metabolites. all fibres increased wet faecal output; an increase in faecal dm output being observed with sugar-beet fibre only. sugar-beet fibre and inulin increased daily water consump ... | 1998 | 9625462 |
| genes required for replication of the 15.5-kilobase rna genome of a plant closterovirus. | a full-length cdna clone of beet yellows closterovirus (byv) was engineered and used to map functions involved in the replication of the viral rna genome and subgenomic rna formation. among 10 open reading frames (orfs) present in byv, orfs 1a and 1b suffice for rna replication and transcription. the proteins encoded in these orfs harbor putative methyltransferase, rna helicase, and rna polymerase domains common to sindbis virus-like viruses and a large interdomain region that is unique to clost ... | 1998 | 9621048 |
| analysis of plastid dna-like sequences within the nuclear genomes of higher plants. | a wide-ranging examination of plastid (pt)dna sequence homologies within higher plant nuclear genomes (promiscuous dna) was undertaken. digestion with methylation-sensitive restriction enzymes and southern analysis was used to distinguish plastid and nuclear dna in order to assess the extent of variability of promiscuous sequences within and between plant species. some species, such as gossypium hirsutum (cotton), nicotiana tabacum (tobacco), and chenopodium quinoa, showed homogenity of these se ... | 1998 | 9615455 |
| nucleic acid and protein elimination during the sugar manufacturing process of conventional and transgenic sugar beets. | the fate of cellular dna during the standard purification steps of the sugar manufacturing process from conventional and transgenic sugar beets was determined. indigenous nucleases of sugar beet cells were found to be active during the first extraction step (raw juice production) which was carried out at 70 degrees c. this and the consecutive steps of the manufacturing process were validated in terms of dna degradation by competitive pcr of added external dna. each step of the process proved to ... | 1998 | 9608751 |
| effect of adding sugar beet fibre and wheat bran to a starch diet on the absorption kinetics of glucose, amino-nitrogen and volatile fatty acids in the pig. | the purpose of this study with the pig was to analyse the influence of the type of dietary fibre on quantitative kinetics of the absorption of nutrients deriving from enzymatic digestion in the small intestine and that of volatile fatty acids (vfa) deriving from microbial digestion in the hindgut influenced by the length of adaptation to the diet. two groups of four pigs were fitted with a device for measuring absorption by simultaneous analysis of the differences in the porto-arterial concentra ... | 1998 | 9606749 |
| detection of nptii (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. | we have developed a novel system for the sensitive detection of nptii genes (kanamycin resistance determinants) including those present in transgenic plant genomes. the assay is based on the recombinational repair of an nptii gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter. uptake of an nptii gene by transformation restores kanamycin resistance. in escherichia coli, promoterless nptii genes provided by electroporation were rescued with high efficiency ... | 1998 | 9604883 |
| nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus. | the rna genome of grapevine leafroll-associated closterovirus-3 (glrav-3) was cloned as a cdna generated from glrav-3-specific dsrna, and a partial genome sequence of 13154 nucleotides (nt) including the 3' terminus was determined. the sequenced portion contained 13 open reading frames (orfs) potentially encoding, in the 5'-3' direction, proteins of > 77 kda (orf1a; helicase, hel), 61 kda (orf1b; rna-dependent rna polymerase, rdrp), 6 kda (orf2), 5 kda (orf3, small transmembrane protein), 59 kda ... | 1998 | 9603346 |
| nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 are similar to beet yellows virus, the closterovirus type member. | the entire genome of grapevine leafroll-associated closterovirus-2 (glrav-2), except the exact 5' terminus, was cloned and sequenced. the sequence encompasses nine open reading frames (orfs) which include, in the 5' to 3' direction, an incomplete orf1a encoding a putative viral polyprotein and eight orfs that encode proteins of 52 kda (orf1b), 6 kda (orf2), 65 kda (orf3), 63 kda (orf4), 25 kda (orf5), 22 kda (orf6), 19 kda (orf7) and 24 kda (orf8) respectively, and 216 nucleotides of the 3' untr ... | 1998 | 9603345 |
| glutamine synthetase isoenzymes, oligomers and subunits from hairy roots of beta vulgaris l. var. lutea. | a cytosolic and a plastidic isoenzyme of glutamine synthetase (gs; ec 6.3.1.2) were separated from hairy roots of beta vulgaris l. var. lutea. the predominant activity was that of cytosolic gs 1; the relative proportion of plastidic gs 2 activity changed, however, depending on the growth conditions. maximum activity of both isoenzymes was measured after growth with no3- as the major n-source. growth with nh4+ as the sole n-source or growth in constant darkness resulted in a significant decrease ... | 1998 | 9599808 |
| [alternative foods: a critical analysis of a proposal for nutritional intervention] | the nationwide use of a 'multimixture' a formula based on alternative foods such as rice and/or wheat bran, sesame and squash seeds, cassava, beet and carrot leaves, several indigenous leafy vegetables, and ground egg shells has been proposed by the national institute of food and nutrition (inan) as an official solution to fight hunger among poor brazilians. the fragile nutritional state of the target population may make technical or ethical questions appear purely academic, yet nutritional, tox ... | 1998 | 9592227 |
| anti-nociceptive and anti-inflammatory effects of some jordanian medicinal plant extracts. | the anti-nociceptive effect of ethanolic extract of 11 traditionally used jordanian plants was studied by using the acetic acid-induced writhing and hot-plate test in mice. the anti-inflammatory effect of these plants was determined by xylene-induced ear oedema in mice and cotton pellet granuloma test in rats. mentha piperita, cinnamomum zeylanicum, apium graveolens, eucalyptus camaldulentis, and ruta graveolens possess an anti-nociceptive effect against both acetic acid-induced writhing and hot ... | 1998 | 9582001 |
| influence of dietary factors on the ph and ammonia emission of slurry from growing-finishing pigs. | we investigated the effects of dietary factors on the ph and the ammonia emission from slurry of growing-finishing pigs. sixteen male hybrid pigs (80 to 90 kg bw) were allotted to one of four diets based on barley-wheat, tapioca, barley-tapioca, and sugar beet pulp. diets were formulated to have similar ne and cp contents and a similar lysine:ne ratio. diets differed in nonstarch polysaccharide content (nsp) and dietary electrolyte balance (deb). urine and feces were daily collected quantitative ... | 1998 | 9581936 |
| isolation and structural characterisation of rhamnogalacturonan oligomers generated by controlled acid hydrolysis of sugar-beet pulp. | controlled acid hydrolysis was applied to a deesterified beet pulp and the resulting soluble fraction was fractionated on a biorad ag 1x8 column eluted by ammonium acetate ph 6 from 0.05 to 2 m. eight retained fractions were obtained, containing almost exclusively gala and rha. three types of oligomers could be identified: homogalacturonans, of which mono-,di- and tri-gala were isolated as individual components, and two series of rhamnogalacturonan (rg) oligomers. one rg oligomer, isolated after ... | 1997 | 9581279 |
| beet yellows closterovirus hsp70-like protein mediates the cell-to-cell movement of a potexvirus transport-deficient mutant and a hordeivirus-based chimeric virus. | it has been suggested that the beet yellows closterovirus (byv)-encoded p65 protein, a homologue of hsp70 cell chaperones, plays a role as a virus movement protein (mp). to test this hypothesis, we used two types of complementation experiments with plant viruses containing the triple gene block (tgb) of mp genes. in one, the byv p65 gene was cloned into a 35s promoter plasmid and introduced into nicotiana benthamiana plants by microprojectile bombardment along with the 35s promoter-driven gus ge ... | 1998 | 9568985 |
| characterization of galactosidases from aspergillus niger: purification of a novel alpha-galactosidase activity. | an enzyme with beta-galactosidase activity and three proteins exhibiting alpha-galactosidase activity were purified from a culture filtrate of aspergillus niger grown on arabinoxylan. beta-galactosidase, optimally active at ph 4 and 60-65 degrees c, was active against p-nitrophenyl-beta-d-galactopyranoside, lactose, and pectic galactan. it was not able to release galactose from sugar beet pectin or lemon pectin. its action on pectic galactan was increased by the presence of beta-galactanase. the ... | 1998 | 9549105 |
| characterization of monoclonal antibodies against cell wall epitopes of the insect pathogenic fungus, nomuraea rileyi: differential binding to fungal surfaces and cross-reactivity with host hemocytes and basement membrane components. | monoclonal antibodies (mabs) were generated against epitopes on yeast-like hyphal bodies and hyphae of the entomopathogenic hyphomycete, nomuraea rileyi. two mabs (4c10, 2h4) bind to epitopes common to both hyphal bodies and hyphae, whereas mab 4e9 binds only to hyphal surfaces. 4c10 and 2h4 appear to be directed towards carbohydrate portions of cell surface mannoproteins, as evidenced by similarities in staining patterns between these mabs and concanavalin a on western blots of n. rileyi cell w ... | 1998 | 9548369 |
| transformation of acinetobacter sp. strain bd413 by transgenic sugar beet dna. | the ability of acinetobacter sp. strain bd413(pfg4 delta nptii) to take up and integrate transgenic plant dna based on homologous recombination was studied under optimized laboratory conditions. restoration of nptii, resulting in kanamycin-resistant transformants, was observed with plasmid dna, plant dna, and homogenates carrying the gene nptii. molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional dna. | 1998 | 9546192 |
| regulation of a plant plasma membrane sucrose transporter by phosphorylation. | the protein phosphatase inhibitor okadaic acid (oa) either provided directly to sugar beet (beta vulgaris l.) leaf discs or infiltrated in the leaf blade rapidly inhibited sucrose uptake. methyl okadaic acid, a biologically inactive analogue of oa, had only a marginal effect on uptake. oa inhibited proton-motive force-driven uptake of sucrose into plasma membrane vesicles, without affecting their proton permeability. oa did not significantly affect the amount of sucrose transporters present in t ... | 1998 | 9539143 |
| the plasma-membrane h(+)-atpase from beet root is inhibited by a calcium-dependent phosphorylation. | several plasma-membrane proteins from beet root (beta vulgaris l.) have been functionally incorporated into reconstituted proteoliposomes. these showed h(+)-atpase activity, measured both as atp hydrolysis and h+ transport. the proton-transport specific activity was 10 times higher than in plasma membranes, and was greatly stimulated by potassium and valinomycin. these proteoliposomes also showed calcium-regulated protein kinase activity. this kinase activity is probably due to a calmodulin-like ... | 1998 | 9530879 |
| fermentation products of sugar-beet fiber by cecal bacteria lower plasma cholesterol concentration in rats. | plasma cholesterol concentration is reduced by feeding some dietary fibers but the mechanism is not fully understood. we examined whether cecal fermentation products are involved in lowering plasma cholesterol by feeding rats a highly fermentable sugar-beet fiber (sbf) in four separate experiments. these were designed to investigate the effects on plasma cholesterol of oral ingestion of fermentation products on plasma cholesterol, the effects of the products in comparison with that of a short-ch ... | 1998 | 9521629 |
| the genomic organization of non-ltr retrotransposons (lines) from three beta species and five other angiosperms. | we have isolated and characterized conserved regions of the reverse transcriptase gene from non-ltr retrotransposons, also called long interspersed nuclear elements (lines), from beta vulgaris, b. lomatogona and b. nana. the novel elements show strong homology to other non-ltr retrotransposons from plants, man and animals. lines are present in all species of the genus beta tested, but there was variation in copy number. analysis by southern hybridization and fluorescent in situ hybridization rev ... | 1998 | 9520275 |
| effect of inclusion of sugar beet pulp, pelleting and season on laying hen performance. | 1. the effect of three metabolisable energy (me) concentrations 12.2, 11.3 and 10.5 mj me/kg and two forms of diet (mash and pellet) on egg production in summer and winter were studied. the different me values were obtained by diluting the 12.2 mj me/kg diet with sugar beet pulp of 2.88 mj/kg dm. in a 3rd summer experiment the water intake of hens on these diets was examined. data were analysed by factorial analysis of variance, and the relationship between performance and dietary energy concent ... | 1997 | 9510998 |
| sugar beet fiber in formula diet reduces postprandial blood glucose, serum insulin and serum hydroxyproline. | the object of the study was to investigate the effects of sugar beet fiber in formula diet on blood glucose, serum insulin and serum hydroxyproline in humans. | 1998 | 9505164 |
| investigation with chitosan-oxalate oxidase-catalase conjugate for degrading oxalate from hyperoxaluric rat chyme. | enteric hyperoxaluria manifests due to hyperabsorption of dietary oxalate, secondary to a variety of chronic gastrointestinal disorders. the potential use of chitosan immobilized oxalate oxidase-catalase conjugate to deplete the oxalate content of food materials, while they are in the digestive tract has been evaluated by treating rat stomach chyme with such an enzyme preparation. oxalate oxidase, obtained from beet stem, was adsorbed on chitosan along with catalase and then cross linked with gl ... | 1997 | 9491647 |
| osmotic stress induces expression of choline monooxygenase in sugar beet and amaranth. | choline monooxygenase (cmo) catalyzes the committing step in the synthesis of glycine betaine, an osmoprotectant accumulated by many plants in response to salinity and drought. to investigate how these stresses affect cmo expression, a spinach (spinacia oleracea l., chenopodiaceae) probe was used to isolate cmo cdnas from sugar beet (beta vulgaris l., chenopodiaceae), a salt- and drought-tolerant crop. the deduced beet cmo amino acid sequence comprised a transit peptide and a 381-residue mature ... | 1998 | 9489025 |
| ecbi and ecbr: homologs of luxi and luxr affecting antibiotic and exoenzyme production by erwinia carotovora subsp. betavasculorum. | erwinia carotovora subsp. betavasculorum ecb168 causes vascular necrosis and root rot of sugar beet and produces an antibiotic(s) that is antagonistic against other erwinia spp. ecbi- mutants of ecb168, each containing a single transposon insertion in the ecbi gene (for erwinia carotovora subsp. betavasculorum inducer), do not produce detectable levels of extracellular protease or antibiotic(s), and express less pectate lyase activity and virulence than the wild-type strain. a plasmid containing ... | 1997 | 9476353 |
| vascular movement of beet necrotic yellow vein virus in beta macrocarpa is probably dependent on an rna 3 sequence domain rather than a gene product. | rnas 1 and 2 of beet necrotic yellow vein virus (bnyvv) carry the functions enabling viral rna replication, cell-to-cell movement, virus assembly and vascular movement of the virus in the systemic host spinacea oleracea. in beta macrocarpa, on the other hand, bnyvv rna 3 is required for vascular movement. replication-competent rna 3 transcripts carrying various point mutations and deletions were coinnoculated with rnas 1 and 2 to young leaves of b. macrocarpa and the ability of the virus to mult ... | 1998 | 9472625 |
| agelastatins c and d, new pentacyclic bromopyrroles from the sponge cymbastela sp., and potent arthropod toxicity of (-)-agelastatin a. | two new agelastatins, c (3) and d (4), along with the known compound (-)-agelastatin a (1), were isolated from the indian ocean sponge cymbastela sp. the structures were determined by analysis of spectroscopic data and chemical correlation of 4 with a common derivative 5, also prepared from 1. agelastatin a (1) exhibited potent activity against brine shrimp (lc50 1.7 ppm) in addition to insecticidal activity against larvae of beet army worm, spodoptera exigua, and corn rootworm, diabrotica undec ... | 1998 | 9461668 |
| suppression of the number of aberrant crypt foci of rat colorectum by ingestion of sugar beet fiber regardless of administration of anti-asialo gm1. | anti-asialo gm1 serum (agm1) reduces natural killer (nk) activity in vitro and in vivo. we investigated the effect of ingestion of sugar beet fiber (sbf) on 1,2-dimethylhydrazine (dmh)-induced aberrant crypt foci (acf) and whether the effect was maintained under nk-reducing conditions by agm1 injection. the ingestion of sbf decreased the number of acf in the colorectum at 4 weeks after treatment with dmh. dietary sbf had a suppressive effect on the formation of acf regardless of the administrati ... | 1997 | 9459172 |
| regulatory sequences of arabidopsis drive reporter gene expression in nematode feeding structures. | in the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. a large collection of transgenic arabidopsis plants was generated. they were transformed with a beta-glucuronidase gene functioning as a promoter tag. three t-dna constructs, pgv1047, p delta gusbin19, and pmog553, were used. early responses to nematode invasion were of primary interest. six lines exhibiting beta-glucu ... | 1997 | 9437858 |
| purification and properties of arfi, an alpha-l-arabinofuranosidase from cytophaga xylanolytica. | an alpha-l-arabinofuranosidase (alpha-l-arabinofuranoside arabinofuranohydrolase [ec 3.2.1.55]; referred to below as arfi) from cytophaga xylanolytica xm3 was purified 85-fold by anion-exchange and hydrophobic interaction column chromatography. the native enzyme had a pi of 6.1 and an apparent molecular mass of 160 to 210 kda, and it appeared to be a trimer or tetramer consisting of 56-kda subunits. with p-nitrophenyl-alpha-l-arabinofuranoside as the substrate, the enzyme exhibited a k(m) of 0.5 ... | 1998 | 9435061 |
| highly efficient induction of sugar beet plant regeneration. | a method for high-frequency in vitro regeneration of adventitious shoots from petiole explants of a tetraploid breeding line 405 of beta vulgaris l. was established. plant regeneration was obtained by direct bud formation from petioles. explants were precultured in a medium containing ms inorganic salts supplemented with gamborg's vitamins, named msb. adventitious shoots were developed when petiole explants excised from 20-30-day-old seedlings grown on msb containing 0.3 mg/l naphthalene acetic ... | 1997 | 9429780 |
| effect of spumol k on cell wall of aspergillus niger strains characterized by different tolerance to toxic compounds of beet molasses. | cell walls in the mycelium of aspergillus niger strains sensitive or resistant to toxic molasses compounds growing in the presence of spumol k were the object of the present studies. although the inhibitory effect of spumol k on the cell wall was noticed in studied mycelia the sensitive strains reacted more strongly. in sensitive strains in spumol k presence lower content of cell wall was accomplished by higher amount of proteins but lower of lipids as well as structural polymers i.e. of glucans ... | 1997 | 9429292 |
| oxidative cross-linking of plasma membrane arabinogalactan proteins. | monoclonal antibodies which recognize carbohydrate in arabinogalactan proteins (agps) have revealed that certain carbohydrate epitopes at the outer plasma membrane surface are developmentally regulated. some epitopes are expressed according to cell position, and agps are thought to play a role in cell-cell interaction during development. this study demonstrates that sugar beet plasma membranes contain two subfamilies of agps, with apparent molecular masses of 82 and 97 kda, and that each subfami ... | 1997 | 9418056 |
| cutting, ageing and expression of plant membrane transporters. | the activity and the expression of sucrose, hexose and amino acid transporters were studied with fresh, cut or aged tissues and plasma membrane vesicles (pmv) of mature sugar beet (beta vulgaris l.) leaves. cutting and ageing both induced an increase of the transcripts coding for sucrose transporters and hexose transporters. no significant effect could be detected on the amino acid transporter transcripts with the probe used (aap1). a polyclonal serum directed against the arabidopsis thaliana su ... | 1997 | 9408174 |
| the faea genes from aspergillus niger and aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides. | we report the cloning and characterization of a gene encoding a ferulic acid esterase, faea, from aspergillus niger and aspergillus tubingensis. the a. niger and a. tubingensis genes have a high degree of sequence identity and contain one conserved intron. the gene product, faea, was overexpressed in wild-type a. tubingensis and a protease-deficient a. niger mutant. overexpression of both genes in wild-type a. tubingensis and an a. niger protease-deficient mutant showed that the a. tubingensis g ... | 1997 | 9406381 |
| beet soil-borne virus rna 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains. | the complete sequence of the 5834 nucleotides of rna 1 of beet soil-borne furovirus (bsbv, ahlum isolate) was determined using a pcr product obtained with primers to highly conserved coding regions for helicase-like proteins in rna 1 of furo-, hordei- and tobraviruses as a starting sequence. unknown parts of the sequence upstream and downstream of this starting sequence were amplified by means of rt-pcr techniques using combinations of specific and random primers. bsbv rna 1 contains one large o ... | 1997 | 9400965 |
| characterization of a putative spodoptera exigua multicapsid nucleopolyhedrovirus helicase gene. | putative baculovirus helicases have been implicated as playing an important role in viral dna replication and host specificity. the spodoptera exigua multicapsid nucleopolyhedrovirus (semnpv) helicase is therefore of interest since the virus only infects the beet army worm. sequence analysis of the semnpv lef5-p39 (mu 46.5-55.1) region, which is collinear with the 39k-lef5 area in autographa californica mnpv (acmnpv), revealed an open reading frame (orf) of 3666 bp potentially encoding a protein ... | 1997 | 9400958 |
| comparison of elisa and rt-pcr for the detection of beet yellows closterovirus in plants and aphids. | a reverse-transcription polymerase chain reaction (rt-pcr) was developed to detect beet yellows closterovirus (byv) in plants and single aphids using primers spanning the conserved regions of the published sequences of the coat protein gene and open reading frames 7 and 8. three total rna extraction procedures were examined and all were found to produce rna of sufficient quality for rt-pcr, although the rna extraction kit supplied by flowgen was found to be the most versatile for the extraction ... | 1997 | 9395136 |
| mercury-induced conformational changes and identification of conserved surface loops in plasma membrane aquaporins from higher plants. topology of pmip31 from beta vulgaris l. | aquaporins are integral membrane proteins occurring in mammals, plants, and microorganisms, which serve as channels that permit the bidirectional passage of water through cellular membranes. higher plants contain abundant levels of aquaporins in both the tonoplast and plasma membrane. aquaporins contain six transmembrane segments with three surface loops located at the apoplastic face of the membrane and two loops at the cytosolic side. in this study, we probed the topology of plasma membrane aq ... | 1997 | 9388202 |
| conformation of the 3'-end of beet necrotic yellow vein benevirus rna 3 analysed by chemical and enzymatic probing and mutagenesis. | secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus rna 3. the structure consists of two stem-loops separated by a single-stranded region. rna 3-derived transcripts were produced containing mutations which either disrupted base pairing in the helices or maintained the helices but with alterations in the base pairing scheme. other mutants contained s ... | 1997 | 9365250 |
| influence of a blend of fructo-oligosaccharides and sugar beet fiber on nutrient digestibility and plasma metabolite concentrations in healthy beagles. | to evaluate effects of a blend of fructo-oligosaccharides and sugar beet fiber (4:1) at 3 incorporation rates on nutrient digestibility and plasma glucose, insulin, alpha-aminonitrogen, urea, cholesterol, and triglycerides concentrations measured weekly in nonfed dogs and during a 360-minute period after a meal. | 1997 | 9361885 |